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https://www.readbyqxmd.com/read/27930821/assessment-of-asymmetrical-dimethylarginine-metabolism-in-patients-with-critical-illness
#1
Rawia A Ghashut, Scott Blackwell, Sylvia Ryan, Laura Willox, Donald C McMillan, John Kinsella, Dinesh Talwar
BACKGROUND: Critically ill patients experience metabolic disorders including hypercatabolic state and hyperglycaemia and these are associated with poor outcome. Hyperglycaemia and asymmetric dimethylarginine (ADMA) are reported to have significant influences on endothelial dysfunction. The aim of the present study was to examine the relationship between plasma asymmetric dimethylarginine (ADMA) and related arginine metabolism in patients with critical illness. MATERIALS AND METHODS: Two venous blood samples (EDTA) (104 patients), on admission and follow up sample in the last day in ICU (died or discharge sample median 7, IQR 6-8, range 5-15)...
December 8, 2016: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/27922179/a-carbon-dioxide-stripping-model-for-mammalian-cell-culture-in-manufacturing-scale-bioreactors
#2
Zizhuo Xing, Amanda M Lewis, Michael C Borys, Zheng Jian Li
Control of carbon dioxide within the optimum range is important in mammalian bioprocesses at the manufacturing scale in order to ensure robust cell growth, high protein yields, and consistent quality attributes. The majority of bioprocess development work is done in laboratory bioreactors, in which carbon dioxide levels are more easily controlled. Some challenges in carbon dioxide control can present themselves when cell culture processes are scaled up, because carbon dioxide accumulation is a common feature due to longer gas-residence time of mammalian cell culture in large scale bioreactors...
December 6, 2016: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/27919225/comparison-of-the-first-three-waves-of-avian-influenza-a-h7n9-virus-circulation-in-the-mainland-of-the-people-s-republic-of-china
#3
Nijuan Xiang, A Danielle Iuliano, Yanping Zhang, Ruiqi Ren, Xingyi Geng, Bili Ye, Wenxiao Tu, Ch Ao Li, Yong Lv, Ming Yang, Jian Zhao, Yali Wang, Fuqiang Yang, Lei Zhou, Bo Liu, Yuelong Shu, Daxin Ni, Zijian Feng, Qun Li
BACKGROUND: H7N9 human cases were first detected in mainland China in March 2013. Circulation of this virus has continued each year shifting to typical winter months. We compared the clinical and epidemiologic characteristics for the first three waves of virus circulation. METHODS: The first wave was defined as reported cases with onset dates between March 31-September 30, 2013, the second wave was defined as October 1, 2013-September 30, 2014 and the third wave was defined as October 1, 2014-September 30, 2015...
December 5, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27918253/placental-growth-factor-alone-or-in-combination-with-soluble-fms-like-tyrosine-kinase-1-as-an-aid-to-the-assessment-of-women-with-suspected-pre-eclampsia-systematic-review-and-economic-analysis
#4
Geoff K Frampton, Jeremy Jones, Micah Rose, Liz Payne
BACKGROUND: Pre-eclampsia (PE) prediction based on blood pressure, presence of protein in the urine, symptoms and laboratory test abnormalities can result in false-positive diagnoses. This may lead to unnecessary antenatal admissions and preterm delivery. Blood tests that measure placental growth factor (PlGF) or the ratio of soluble fms-like tyrosine kinase 1 (sFlt-1) to PlGF could aid prediction of PE if either were added to routine clinical assessment or used as a replacement for proteinuria testing...
November 2016: Health Technology Assessment: HTA
https://www.readbyqxmd.com/read/27911122/an-update-on-the-use-of-cerebrospinal-fluid-analysis-as-a-diagnostic-tool-in-multiple-sclerosis
#5
Matteo Gastaldi, Elisabetta Zardini, Diego Franciotta
Intrathecal B-lymphocyte activation is a hallmark of multiple sclerosis (MS), a multi-factorial inflammatory-demyelinating disease of the central nervous system. Such activation has a counterpart in the cerebrospinal fluid (CSF) oligoclonal IgG bands (OCB), whose diagnostic role in MS has been downgraded within the current McDonald's criteria. With a theoretico-practical approach, the authors review the physiopathological basis of the CSF dynamics, and the state-of-the-art of routine CSF analysis and CSF biomarkers in MS...
December 2, 2016: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/27910336/high-intensity-laser-accelerated-ion-beam-produced-from-cryogenic-micro-jet-target
#6
M Gauthier, J B Kim, C B Curry, B Aurand, E J Gamboa, S Göde, C Goyon, A Hazi, S Kerr, A Pak, A Propp, B Ramakrishna, J Ruby, O Willi, G J Williams, C Rödel, S H Glenzer
We report on the successful operation of a newly developed cryogenic jet target at high intensity laser-irradiation. Using the frequency-doubled Titan short pulse laser system at Jupiter Laser Facility, Lawrence Livermore National Laboratory, we demonstrate the generation of a pure proton beam a with maximum energy of 2 MeV. Furthermore, we record a quasi-monoenergetic peak at 1.1 MeV in the proton spectrum emitted in the laser forward direction suggesting an alternative acceleration mechanism. Using a solid-density mixed hydrogen-deuterium target, we are also able to produce pure proton-deuteron ion beams...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27910032/cytogenetic-nomenclature-and-reporting
#7
Marian Stevens-Kroef, Annet Simons, Katrina Rack, Rosalind J Hastings
A standardized nomenclature is critical for the accurate and consistent description of genomic changes as identified by karyotyping, fluorescence in situ hybridization and microarray. The International System for Human Cytogenomic Nomenclature (ISCN) is the central reference for the description of karyotyping, FISH, and microarray results, and provides rules for describing cytogenetic and molecular cytogenetic findings in laboratory reports. These laboratory reports are documents to the referring clinician, and should be clear, accurate and contain all information relevant for good interpretation of the cytogenetic findings...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27906976/a-laboratory-critical-incident-and-error-reporting-system-for-experimental-biomedicine
#8
Ulrich Dirnagl, Ingo Przesdzing, Claudia Kurreck, Sebastian Major
We here propose the implementation of a simple and effective method to enhance the quality of basic and preclinical academic research: critical incident reporting (CIR). CIR has become a standard in clinical medicine but to our knowledge has never been implemented in the context of academic basic research. We provide a simple, free, open-source software tool for implementing a CIR system in research groups, laboratories, or large institutions (LabCIRS). LabCIRS was developed, tested, and implemented in our multidisciplinary and multiprofessional neuroscience research department...
December 2016: PLoS Biology
https://www.readbyqxmd.com/read/27904162/impact-of-catheterization-lab-computer-software-settings-on-hemodynamic-assessment-of-aortic-stenosis
#9
Zaher Fanari, Anitha Rajamanickam, Mathew Grove, Sumaya Hammami, Cassie Walls, Paul Kolm, William Weintraub, Andrew J Doorey
BACKGROUND: Accurate assessment of Cardiac Output (CO) is a critical measurement in the calculation of aortic valve area (AVA). Due to the known inaccuracy of estimated Fick calculations, many measure thermodilution (TD) CO as well due to previous studies showing better correlation with the gold standard direct CO. Previous studies showed suboptimal correlation between both methods. Most physicians assume that the TD CO is chosen by catheterization laboratory software for AVA evaluation...
July 2016: Delaware Medical Journal
https://www.readbyqxmd.com/read/27898218/global-health-security-building-capacities-for-early-event-detection-epidemiologic-workforce-and-laboratory-response
#10
S Arunmozhi Balajee, Ray Arthur, Anthony W Mounts
The Global Health Security Agenda (GHSA) was launched in February 2014 to bring countries with limited capacity into compliance with the International Health Regulations (IHR) (2005). Recent international public health events, such as the appearance of Middle Eastern respiratory syndrome coronavirus and the reappearance of Ebola in West Africa, have highlighted the importance of early detection of disease events and the interconnectedness of countries. Surveillance systems that allow early detection and recognition of signal events, a public health infrastructure that allows rapid notification and information sharing within countries and across borders, a trained epidemiologic workforce, and a laboratory network that can respond appropriately and rapidly are emerging as critical components of an early warning and response system...
November 29, 2016: Health Security
https://www.readbyqxmd.com/read/27882067/bioinformatic-identification-of-rare-codon-clusters-rccs-in-hbv-genome-and-evaluation-of-rccs-in-proteins-structure-of-hepatitis-b-virus
#11
Mojtaba Mortazavi, Mohammad Zarenezhad, Saeid Gholamzadeh, Seyed Moayed Alavian, Mohammad Ghorbani, Reza Dehghani, Abdorrasoul Malekpour, Mohammadhasan Meshkibaf, Ali Fakhrzad
BACKGROUND: Hepatitis B virus (HBV) as an infectious disease that has nine genotypes (A - I) and a 'putative' genotype J. OBJECTIVES: The aim of this study was to identify the rare codon clusters (RCC) in the HBV genome and to evaluate these RCCs in the HBV proteins structure. METHODS: For detection of protein family accession numbers (Pfam) in HBV proteins, the UniProt database and Pfam search tool were used. Protein family accession numbers is a comprehensive and accurate collection of protein domains and families...
October 2016: Hepatitis Monthly
https://www.readbyqxmd.com/read/27881445/fostering-21st-century-evolutionary-reasoning-teaching-tree-thinking-to-introductory-biology-students
#12
Laura R Novick, Kefyn M Catley
The ability to interpret and reason from Tree of Life (ToL) diagrams has become a vital component of science literacy in the 21st century. This article reports on the effectiveness of a research-based curriculum, including an instructional booklet, laboratory, and lectures, to teach the fundamentals of such tree thinking in an introductory biology class for science majors. We present the results of a study involving 117 undergraduates who received either our new research-based tree-thinking curriculum or business-as-usual instruction...
2016: CBE Life Sciences Education
https://www.readbyqxmd.com/read/27873009/systematic-review-and-need-assessment-of-pediatric-trauma-outcome-benchmarking-tools-for-low-resource-settings
#13
REVIEW
Etienne St-Louis, Jade Séguin, Daniel Roizblatt, Dan Leon Deckelbaum, Robert Baird, Tarek Razek
INTRODUCTION: Trauma is a leading cause of mortality and disability in children worldwide. The World Health Organization reports that 95% of all childhood injury deaths occur in Low-Middle-Income Countries (LMIC). Injury scores have been developed to facilitate risk stratification, clinical decision making, and research. Trauma registries in LMIC depend on adapted trauma scores that do not rely on investigations that require unavailable material or human resources. We sought to review and assess the existing trauma scores used in pediatric patients...
November 21, 2016: Pediatric Surgery International
https://www.readbyqxmd.com/read/27869364/assessing-the-reliability-of-ecotoxicological-studies-an-overview-of-current-needs-and-approaches
#14
Cta Moermond, A Beasley, R Breton, M Junghans, R Laskowski, K Solomon, H Zahner
In general, reliable studies are well designed, performed, and enough details on study design and performance are reported to assess the study. For hazard and risk assessment in various legal frameworks, many different types of ecotoxicity studies need to be evaluated for reliability. These studies vary in study design, methodology, quality, and in the level of detail reported (e.g., reviews, peer-reviewed research papers, or industry-sponsored studies documented under Good Laboratory Practice (GLP) guidelines)...
November 21, 2016: Integrated Environmental Assessment and Management
https://www.readbyqxmd.com/read/27864887/ethical-use-of-animal-models-in-musculoskeletal-research
#15
Matthew J Allen Vet, Kurt D Hankenson, Laurie Goodrich, Gregory P Boivin, Brigitte von Rechenberg
The use of animals in research is under increasing scrutiny from the general public, funding agencies and regulatory authorities. Our ability to continue to perform in-vivo studies in laboratory animals will be critically determined by how researchers respond to this new reality. This Perspectives article summarizes recent and ongoing initiatives within ORS and allied organizations to ensure that musculoskeletal research is performed to the highest ethical standards. It goes on to present an overview of the practical application of the 3Rs (reduction, refinement and replacement) into experimental design and execution, and discusses recent guidance with regard to improvements in the way in which animal data are reported in publications...
November 19, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/27864449/what-are-the-critical-steps-in-processing-blood-cultures-a-prospective-audit-evaluating-current-practice-of-reporting-blood-cultures-in-a-centralised-laboratory-serving-secondary-care-hospitals
#16
Manjula Meda, James Clayton, Reela Varghese, Jayakeerthi Rangaiah, Clive Grundy, Farnaz Dashti, David Garner, Katherine Groves, Karen Fitzmaurice, E Hutley
AIMS: To assess current procedures of processing positive blood cultures against national standards with an aim to evaluate its clinical impact and to determine the utility of currently available rapid identification and susceptibility tests in processing of blood cultures. METHODS: Blood cultures from three secondary care hospitals, processed at a centralised laboratory, were prospectively audited. Data regarding processing times, communication with prescribers, changes to patient management and mortality within 30 days of a significant blood culture were collected in a preplanned pro forma for a 4-week period...
November 18, 2016: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/27863758/determination-of-reference-ranges-for-immature-platelet-and-reticulocyte-fractions-and-reticulocyte-hemoglobin-equivalent
#17
Iuri Vicente Camargo Morkis, Mariela Granero Farias, Luciana Scotti
INTRODUCTION: The immature platelet and immature reticulocyte fractions represent the ratios of platelets and reticulocytes recently released into the circulation and thus with higher RNA content. They are considered early indicators of bone marrow recovery. OBJECTIVE: The aim of this study was to determine the reference ranges for the immature platelet and reticulocyte fractions of hematologically normal individuals in a university hospital. METHODS: Venous blood samples collected in ethylenediaminetetraacetic acid K3 were analyzed using a Sysmex XE-5000™ analyzer...
October 2016: Revista Brasileira de Hematologia e Hemoterapia
https://www.readbyqxmd.com/read/27863513/epidemiology-of-rubella-virus-cases-in-the-pre-vaccination-era-of-ethiopia-2009-2015
#18
Mekonen Getahun, Berhane Beyene, Kathleen Gallagher, Ayesheshem Ademe, Birke Teshome, Mesfin Tefera, Anjelo Asha, Aklog Afework, Esete Assefa, Yoseph HaileMariam, Yonas HaileGiorgis, Hiwot Ketema, Dejenie Shiferaw, Ayenachew Bekele, Daddi Jima, Amha Kebede
BACKGROUND: Rubella is a common mild rash illness caused by rubella virus. The majority of infections occur in children and young adults. The infection is the cause of a serious birth defect known as Congenital Rubella Syndrome (CRS) when a woman acquires infection early in pregnancy. Ethiopia has not yet established rubella virus surveillance and has not yet introduced rubella vaccine into the routine immunization program. We characterize the epidemiology of laboratory confirmed rubella virus cases collected through measles surveillance from 2009 to 2015 to better understand the burden of the disease in the country...
November 18, 2016: BMC Public Health
https://www.readbyqxmd.com/read/27861622/rapid-and-reliable-hplc-method-for-the-simultaneous-determination-of-dihydroxyacetone-methylglyoxal-and-5-hydroxymethylfurfural-in-leptospermum-honeys
#19
Matthew Pappalardo, Linda Pappalardo, Peter Brooks
A reliable determination of dihydroxyacetone, methylglyoxal and 5-hydroxymethylfurfural is essential to establishing the commercial value and antimicrobial potential of honeys derived from the Leptospermum species endemic to Australia and New Zealand. We report a robust method for quantitation of all three compounds in a single HPLC run. Honey samples (n = 6) that are derivatized with o-(2,3,4,5,6-Pentafluorobenzyl) hydroxylamine were quantitated against a stable anisole internal standard. Linear regression analysis was performed using calibration standards for each compound (n = 6) and results indicated a high degree of accuracy (R2 = 0...
2016: PloS One
https://www.readbyqxmd.com/read/27836355/exacerbation-of-hepatitis-e-virus-infection-during-anti-tnf%C3%AE-treatment
#20
Patrick Behrendt, Stefan Lüth, Werner Dammermann, Svenja Drave, Richard J P Brown, Daniel Todt, Ulrike Schnoor, Eike Steinmann, Heiner Wedemeyer, Sven Pischke, Christof Iking-Konert
Chronic hepatitis E virus (HEV) infection may occur in immunocompromised patients. Previous studies report that different immunosuppressive agents interfere with viral replication. However, the role of TNFα in HEV infection is currently unknown. Here, we describe a case of severe exacerbation of a chronic HEV infection in a patient undergoing treatment with a TNFα-inhibitor for psoriatic arthritis despite potent anti-HEV T-cell responses. We used state-of-the-art HEV cell culture methods to test antiviral effects of different drugs and a cytokine release assay to assess HEV specific T cell immunity...
November 8, 2016: Joint, Bone, Spine: Revue du Rhumatisme
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