keyword
MENU ▼
Read by QxMD icon Read
search

Critical labs

keyword
https://www.readbyqxmd.com/read/28440912/performance-of-in-silico-tools-for-the-evaluation-of-p16ink4a-cdkn2a-variants-in-cagi
#1
Marco Carraro, Giovanni Minervini, Manuel Giollo, Yana Bromberg, Emidio Capriotti, Rita Casadio, Roland Dunbrack, Lisa Elefanti, Pietro Fariselli, Carlo Ferrari, Julian Gough, Panagiotis Katsonis, Emanuela Leonardi, Olivier Lichtarge, Chiara Menin, Pier Luigi Martelli, Abhishek Niroula, Lipika R Pal, Susanna Repo, Maria Chiara Scaini, Mauno Vihinen, Qiong Wei, Qifang Xu, Yuedong Yang, Yizhou Yin, Jan Zaucha, Huiying Zhao, Yaoqi Zhou, Steven E Brenner, John Moult, Silvio C E Tosatto
Correct phenotypic interpretation of variants of unknown significance for cancer-associated genes is a diagnostic challenge as genetic screenings gain in popularity in the next-generation sequencing era. The Critical Assessment of Genome Interpretation (CAGI) experiment aims to test and define the state of the art of genotype-phenotype interpretation. Here, we present the assessment of the CAGI p16INK4a challenge. Participants were asked to predict the effect on cellular proliferation of ten variants for the p16INK4a tumor suppressor, a cyclin-dependent kinase inhibitor encoded by the CDKN2A gene...
April 25, 2017: Human Mutation
https://www.readbyqxmd.com/read/28425974/on-the-edge-of-research-and-technological-application-a-critical-review-of-electromethanogenesis
#2
REVIEW
Ramiro Blasco-Gómez, Pau Batlle-Vilanova, Marianna Villano, Maria Dolors Balaguer, Jesús Colprim, Sebastià Puig
The conversion of electrical current into methane (electromethanogenesis) by microbes represents one of the most promising applications of bioelectrochemical systems (BES). Electromethanogenesis provides a novel approach to waste treatment, carbon dioxide fixation and renewable energy storage into a chemically stable compound, such as methane. This has become an important area of research since it was first described, attracting different research groups worldwide. Basics of the process such as microorganisms involved and main reactions are now much better understood, and recent advances in BES configuration and electrode materials in lab-scale enhance the interest in this technology...
April 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28416420/identification-of-endogenous-biomarkers-to-predict-the-propensity-of-drug-candidates-to-cause-hepatic-or-renal-transporter-mediated-ddis
#3
REVIEW
Xiaoyan Chu, Grace Hoyee Chan, Raymond Evers
Drug transporters expressed in liver and kidney play a critical role in the elimination of a wide range of drugs and xenobiotics and inhibition of these transporters may therefore cause clinically significant drug-drug interactions (DDIs). Currently, in vitro transporter inhibition data are used to assess the risk that a drug candidate may act as an inhibitor of a transporter in patients at clinically relevant exposures. However, this approach is hampered by low confidence in in vitro to in vivo extrapolations, and large inter-system and inter-lab variability in in vitro data...
April 14, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28414907/cell-isolation-and-recovery-using-hollow-glass-microspheres-coated-with-nanolayered-films-for-applications-in-resource-limited-settings
#4
Ziye Dong, Caroline C Ahrens, Dan Yu, Zhenya Ding, HyunTaek Lim, Wei Li
Established cell isolation and purification techniques such as fluorescence-activated cell sorting (FACS), isolation through magnetic micro/nanoparticles, and recovery via microfluidic devices have limited application as disposable technologies appropriate for point-of-care use in remote areas where lab equipment as well as electrical, magnetic, and optical sources are restricted. We report a simple yet effective method for cell isolation and recovery that requires neither specialized lab equipment nor any form of power source...
April 26, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28409998/suitability-of-various-dna-extraction-methods-for-a-traditional-chinese-paocai-system
#5
Nan Zhao, Jialiang Cai, Chuchu Zhang, Zhuang Guo, Wenwei Lu, Bo Yang, Feng-Wei Tian, Xiao-Ming Liu, Hao Zhang, Wei Chen
Traditional paocai brine (PB), which is continuously propagated by back-slopping and contains various species of lactic acid bacteria (LAB), is critical for the flavor of paocai. Culture-independent approaches are commonly used to investigate the microbial communities of fermented food. To evaluate the influence of different DNA (DNA) extraction methods on estimates of bacterial community profiles from 4 PBs, the lysis efficiency, DNA yield, purity and denaturing gradient gel electrophoresis (DGGE) profiles of V3 region of a 16S ribosomal ribonucleic acid gene were acquired...
April 14, 2017: Bioengineered
https://www.readbyqxmd.com/read/28407781/early-identification-of-patients-requiring-massive-transfusion-embolization-or-hemostatic-surgery-for-traumatic-hemorrhage-a-systematic-review-protocol
#6
Alexandre Tran, Maher Matar, Ewout W Steyerberg, Jacinthe Lampron, Monica Taljaard, Christian Vaillancourt
BACKGROUND: Hemorrhage is a major cause of early mortality following a traumatic injury. The progression and consequences of significant blood loss occur quickly as death from hemorrhagic shock or exsanguination often occurs within the first few hours. The mainstay of treatment therefore involves early identification of patients at risk for hemorrhagic shock in order to provide blood products and control of the bleeding source if necessary. The intended scope of this review is to identify and assess combinations of predictors informing therapeutic decision-making for clinicians during the initial trauma assessment...
April 13, 2017: Systematic Reviews
https://www.readbyqxmd.com/read/28398525/a-longitudinal-analysis-of-data-quality-in-a-large-pediatric-data-research-network
#7
Ritu Khare, Levon Utidjian, Byron J Ruth, Michael G Kahn, Evanette Burrows, Keith Marsolo, Nandan Patibandla, Hanieh Razzaghi, Ryan Colvin, Daksha Ranade, Melody Kitzmiller, Daniel Eckrich, L Charles Bailey
Objective: PEDSnet is a clinical data research network (CDRN) that aggregates electronic health record data from multiple children's hospitals to enable large-scale research. Assessing data quality to ensure suitability for conducting research is a key requirement in PEDSnet. This study presents a range of data quality issues identified over a period of 18 months and interprets them to evaluate the research capacity of PEDSnet. Materials and Methods: Results were generated by a semiautomated data quality assessment workflow...
April 8, 2017: Journal of the American Medical Informatics Association: JAMIA
https://www.readbyqxmd.com/read/28394617/understanding-the-impact-of-water-on-the-miscibility-and-microstructure-of-amorphous-solid-dispersions-an-afm-lcr-and-tem-edx-study
#8
Na Li, Christopher J Gilpin, Lynne S Taylor
Miscibility is critical for amorphous solid dispersions (ASDs). Phase-separated ASDs are more prone to crystallization, and thus can lose their solubility advantage leading to product failure. Additionally, dissolution performance can be diminished as a result of phase separation in the ASD matrix. Water is known to induce phase separation during storage for some ASDs. However, the impact of water introduced during preparation has not been as thoroughly investigated to date. The purpose of this study was to develop a mechanistic understanding of the effect of water on the phase behavior and microstructure of ASDs...
April 19, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28390801/the-gap-junction-protein-innexin3-is-required-for-eye-disc-growth-in-drosophila
#9
Mélisande Richard, Reinhard Bauer, Gaia Tavosanis, Michael Hoch
The Drosophila compound eye develops from a bilayered epithelial sac composed of an upper peripodial epithelium layer and a lower disc proper, the latter giving rise to the eye itself. During larval stages, complex signalling events between the layers contribute to the control of cell proliferation and differentiation in the disc. Previous work in our lab established the gap junction protein Innexin2 (Inx2) as crucial for early larval eye disc growth. By analysing the contribution of other Innexins to eye size control, we have identified Innexin3 (Inx3) as an important growth regulator...
April 5, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28389681/-adna-research-from-a%C3%A2-historical-perspective
#10
Elsbeth Bösl
aDNA studies are a cooperative field of research with a broad range of applications including evolutionary biology, genetics, anthropology and archaeology. Scientists are using ancient molecules as source material for historical questions. Colleagues from the humanities are observing this with both interest and concern because aDNA research is affecting academic identities and both concepts of history and historiography. aDNA research developed in a way that can be described as a Hype Cycle (Chackie Fenn)...
April 7, 2017: NTM
https://www.readbyqxmd.com/read/28386054/acute-myocardial-infarction-due-to-coronary-artery-embolism-in-a-22-year-old-woman-with-mitral-stenosis-with-atrial-fibrillation-under-warfarinization-successful-management-with-anticoagulation
#11
Santosh Kumar Sinha, Mukesh Jitendra Jha, Mahmadula Razi, Vikash Chaturvedi, Yatish Besthenahalli Erappa, Shravan Singh, Vikas Mishra, Dibbendhu Khanra, Karandeep Singh
BACKGROUND Coronary artery embolization is an exceedingly rare cause of myocardial infarction, but a few cases in association with prosthetic mechanical valves have been reported. We report a case of embolic myocardial infarction caused by a thrombus in the left atrium with deranged coagulation profile in a patient with critical mitral stenosis under warfarinization. CASE REPORT A 22-year-old woman was taken to the catheterization lab for early coronary intervention in lieu of non-ST elevation myocardial infarction...
April 7, 2017: American Journal of Case Reports
https://www.readbyqxmd.com/read/28382769/the-blindfolded-code-training-exercise
#12
Rami Ahmed, Kate Hughes, Patrick Hughes
BACKGROUND: Communication errors are the leading cause of preventable patient harm in medicine. There is little description in the literature of advanced techniques to teach crisis resource management (CRM) communication strategies, or how to improve real-time ineffective behaviour or habits that may persist despite appropriate behaviour and leadership witnessed in the simulation lab. CONTEXT: This article presents an advanced teaching technique to force the adoption of closed-loop communication strategies...
April 5, 2017: Clinical Teacher
https://www.readbyqxmd.com/read/28379956/detecting-and-enumerating-soil-transmitted-helminth-eggs-in-soil-new-method-development-and-results-from-field-testing-in-kenya-and-bangladesh
#13
Lauren Steinbaum, Laura H Kwong, Ayse Ercumen, Makeda S Negash, Amira J Lovely, Sammy M Njenga, Alexandria B Boehm, Amy J Pickering, Kara L Nelson
Globally, about 1.5 billion people are infected with at least one species of soil-transmitted helminth (STH). Soil is a critical environmental reservoir of STH, yet there is no standard method for detecting STH eggs in soil. We developed a field method for enumerating STH eggs in soil and tested the method in Bangladesh and Kenya. The US Environmental Protection Agency (EPA) method for enumerating Ascaris eggs in biosolids was modified through a series of recovery efficiency experiments; we seeded soil samples with a known number of Ascaris suum eggs and assessed the effect of protocol modifications on egg recovery...
April 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28377708/increased-evoked-potentials-to-arousing-auditory-stimuli-during-sleep-implication-for-the-understanding-of-dream-recall
#14
Raphael Vallat, Tarek Lajnef, Jean-Baptiste Eichenlaub, Christian Berthomier, Karim Jerbi, Dominique Morlet, Perrine M Ruby
High dream recallers (HR) show a larger brain reactivity to auditory stimuli during wakefulness and sleep as compared to low dream recallers (LR) and also more intra-sleep wakefulness (ISW), but no other modification of the sleep macrostructure. To further understand the possible causal link between brain responses, ISW and dream recall, we investigated the sleep microstructure of HR and LR, and tested whether the amplitude of auditory evoked potentials (AEPs) was predictive of arousing reactions during sleep...
2017: Frontiers in Human Neuroscience
https://www.readbyqxmd.com/read/28376609/simulation-training-in-upper-tract-endourology-myth-or-reality-critical-analysis-of-the-literature
#15
Catherine E Lovegrove, Takashige Abe, Abdullatif Aydin, Domenico Veneziano, Kemal Sarica, M Shamim Khan, Prokar Dasgupta, Kamran Ahmed
Surgeons training in the twenty-first century are subject to a myriad of pressures, as has always been the case within surgical training. These include reduced hours available for training and increased threat of litigation against their operating practice. The Halstedian approach of "see one, do one, teach one" has been replaced within surgical training and simulation has become established to enable urology trainees to develop technical and non-technical skills away from the operating room. With the primary focus as patient safety and increasing operating skill, "simulation training" encompasses several modalities...
March 30, 2017: Minerva Urologica e Nefrologica, the Italian Journal of Urology and Nephrology
https://www.readbyqxmd.com/read/28352491/acquired-factor-viii-deficiency-two-case-reports-and-a-review-of-literature
#16
Lan Mo, George C Bao
BACKGROUND: Acquired factor VIII (FVIII) deficiency, or acquired hemophilia A (AHA), is a rare autoimmune disorder involving antibody-mediated depletion of coagulation FVIII, leading to severe, life-threatening bleeding. The condition is often associated with other autoimmune disorders, and its treatment involves replacement of FVIII and various modes of immunosuppression. Recently, a few noteworthy therapeutic advances have been made. We present two cases of severe AHA in Chinese women...
2017: Experimental Hematology & Oncology
https://www.readbyqxmd.com/read/28351359/conveying-practical-clinical-skills-with-the-help-of-teaching-associates-a-randomised-trial-with-focus-on-the-long-term-learning-retention
#17
Sebastian H Hoefer, Jasmina Sterz, Bernd Bender, Maria-Christina Stefanescu, Marius Theis, Felix Walcher, Robert Sader, Miriam Ruesseler
BACKGROUND: Ensuring that all medical students achieve adequate clinical skills remains a challenge, yet the correct performance of clinical skills is critical for all fields of medicine. This study analyzes the influence of receiving feedback by teaching associates in the context of achieving and maintaining a level of expertise in complex head and skull examination. METHODS: All third year students at a German university who completed the obligatory surgical skills lab training and surgical clerkship participated in this study...
March 28, 2017: BMC Medical Education
https://www.readbyqxmd.com/read/28347948/laser-microfabrication-of-a-microheater-chip-for-cell-culture-outside-a-cell-incubator
#18
Daniel Nieto, Peter McGlynn, María de la Fuente, Rafael Lopez-Lopez, Gerard M O'connor
Microfluidic chips have demonstrated their significant application potentials in microbiological processing and chemical reactions, with the goal of developing monolithic and compact chip-sized multifunctional systems. Heat generation and thermal control are critical in some of the biochemical processes. The paper presents a laser direct-write technique for rapid prototyping and manufacturing of microheater chips and its applicability for lab-on-a-chip cell culture outside a cell incubator. The aim of the microheater is to take the role of conventional incubators for cell culture for facilitating microscopic observation and/or other online monitoring activities during cell culture and provides portability of cell culture operation...
March 22, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28345437/clinical-profile-dosing-and-quality-of-life-outcomes-in-primary-immune-deficiency-patients-treated-at-home-with-immunoglobulin-g-data-from-the-ideal-patient-registry
#19
Sean Kearns, Loretta Kristofek, William Bolgar, Luqman Seidu, Samantha Kile
BACKGROUND: Patients with primary immune deficiency (PID) often require immunoglobulin G (IgG, commonly referred to as Ig) replacement therapy to prevent infections and associated comorbidities. Ig therapy can be given either through intravenous or subcutaneous routes, and both can be done in the home setting. There is limited information available on the real-world diagnosis, management, and outcomes of this patient population, given the variable disease presentation and treatment options...
April 2017: Journal of Managed Care & Specialty Pharmacy
https://www.readbyqxmd.com/read/28328885/introducing-therioepistemology-the-study-of-how-knowledge-is-gained-from-animal-research
#20
REVIEW
Joseph P Garner, Brianna N Gaskill, Elin M Weber, Jamie Ahloy-Dallaire, Kathleen R Pritchett-Corning
This focus issue of Lab Animal coincides with a tipping point in biomedical research. For the first time, the scale of the reproducibility and translatability crisis is widely understood beyond the small cadre of researchers who have been studying it and the pharmaceutical and biotech companies who have been living it. Here we argue that an emerging literature, including the papers in this focus issue, has begun to congeal around a set of recurring themes, which themselves represent a paradigm shift. This paradigm shift can be characterized at the micro level as a shift from asking "what have we controlled for in this model?" to asking "what have we chosen to ignore in this model, and at what cost?" At the macro level, it is a shift from viewing animals as tools (the furry test tube), to viewing them as patients in an equivalent human medical study...
March 22, 2017: Lab Animal
keyword
keyword
93874
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"