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https://www.readbyqxmd.com/read/29770140/seventeen-year-journey-working-with-a-master
#1
Jinfang Zhu
It had been a great honor for me to work with the late Dr. William E. Paul for 17 years in the Laboratory of Immunology (LI) from 1998 until his passing in 2015. He was such a master in the immunology field. Under his outstanding guidance, my research has been focusing on transcriptional regulation of T helper (Th) cell differentiation, especially, on the role of a master transcription factor GATA3 during Th2 cell differentiation. Just as enormous scientific contributions of Dr. Paul (we all call him Bill) to the immunology community are far beyond his serving as the Chief of the LI, GATA3 also plays important roles in different lymphocytes at various developmental stages besides its critical functions in Th2 cells...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29767357/a-guide-for-designing-and-analyzing-rna-seq-data
#2
Aniruddha Chatterjee, Antonio Ahn, Euan J Rodger, Peter A Stockwell, Michael R Eccles
The identity of a cell or an organism is at least in part defined by its gene expression and therefore analyzing gene expression remains one of the most frequently performed experimental techniques in molecular biology. The development of the RNA-Sequencing (RNA-Seq) method allows an unprecedented opportunity to analyze expression of protein-coding, noncoding RNA and also de novo transcript assembly of a new species or organism. However, the planning and design of RNA-Seq experiments has important implications for addressing the desired biological question and maximizing the value of the data obtained...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29765800/surface-characterization-of-nanoparticles-using-near-field-light-scattering
#3
Eunsoo Yoo, Yizhong Liu, Chukwuazam A Nwasike, Sebastian R Freeman, Brian C DiPaolo, Bernardo Cordovez, Amber L Doiron
The effect of nanoparticle surface coating characteristics on colloidal stability in solution is a critical parameter in understanding the potential applications of nanoparticles, especially in biomedicine. Here we explored the modification of the surface of poly(ethylene glycol)-coated superparamagnetic iron oxide nanoparticles (PEG-SPIOs) with the synthetic pseudotannin polygallol via interpolymer complexation (IPC). Changes in particle size and zeta potential were indirectly assessed via differences between PEG-SPIOs and IPC-SPIOs in particle velocity and scattering intensity using near-field light scattering...
2018: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/29764453/ampa-ergic-regulation-of-amyloid-%C3%AE-levels-in-an-alzheimer-s-disease-mouse-model
#4
Jane C Hettinger, Hyo Lee, Guojun Bu, David M Holtzman, John R Cirrito
BACKGROUND: Extracellular aggregation of the amyloid-β (Aβ) peptide into toxic multimers is a key event in Alzheimer's disease (AD) pathogenesis. Aβ aggregation is concentration-dependent, with higher concentrations of Aβ much more likely to form toxic species. The processes that regulate extracellular levels of Aβ therefore stand to directly affect AD pathology onset. Studies from our lab and others have demonstrated that synaptic activity is a critical regulator of Aβ production through both presynaptic and postsynaptic mechanisms...
May 15, 2018: Molecular Neurodegeneration
https://www.readbyqxmd.com/read/29758024/hatchling-survival-to-breeding-age-in-northern-pine-snakes-pituophis-melanoleucus-in-the-new-jersey-pine-barrens-human-effects-on-recruitment-from-1986-to-2017
#5
Joanna Burger, Robert T Zappalorti, Michael Gochfeld
To conserve threatened/endangered species, we need to understand the factors contributing to reproductive success and recruitment to reproductive stage. Obtaining this information is difficult for snakes because they are secretive, are not easy to locate at the same stage each year, and are sometimes sparsely distributed. We determined nest fate, hatchling growth and survival to age 5 years, and recruitment to breeding age of Northern Pine Snakes (Pituophis melanoleucus) in New Jersey Pine Barrens from 1986 to 2017...
2018: PloS One
https://www.readbyqxmd.com/read/29753036/explaining-the-amount-and-consistency-of-medical-care-and-self-management-support-in-asthma-a-survey-of-primary-care-providers-in-france-and-the-united-kingdom
#6
Marijn de Bruin, Alexandra L Dima, Nathalie Texier, Eric van Ganse
BACKGROUND: The quality of asthma primary care may vary between countries, health care practices, and health care professionals (HCPs). Identifying and explaining such differences is critical for health services improvement. OBJECTIVES: To examine the quality of asthma primary care in France and United Kingdom, and identify within-country and between-country predictors amenable to intervention. METHODS: An online questionnaire to capture asthma medical care and self-management support, practice characteristics, and psychosocial determinants, was completed by 276 HCPs...
May 9, 2018: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/29748188/factors-influencing-the-decision-to-proceed-to-firmware-upgrades-to-implanted-pacemakers-for-cybersecurity-risk-mitigation
#7
Leslie A Saxon, Niraj Varma, Laurence M Epstein, Leonard I Ganz, Andrew E Epstein
In August 2017, the first major recall for cybersecurity vulnerabilities in pacemakers capable of remote connectivity was released that impacted 465,000 United States patients.1,2 The FDA approved a firmware update designed by the manufacturer of the devices as a remediation (Abbott , formally St. Jude Medical). The recall was in response to the public disclosure of vulnerability by an investment firm and produced in a lab environment that could allow an unauthorized party in close proximity to a patient to impact the performance of the device or modify device settings via radio frequency communication...
May 10, 2018: Circulation
https://www.readbyqxmd.com/read/29744657/transcatheter-intervention-of-coronary-obstructions-in-infants-children-and-young-adults
#8
Ryan Callahan, James E Lock, Pinak B Shah, Audrey C Marshall
This study assesses the characteristics of coronary obstructions that underwent transcatheter intervention in a pediatric catheterization laboratory, the procedural details, and patient outcomes. Acute cardiac failure due to coronary obstructions in children is rare. The role of catheter based intervention is largely unreported. Single center retrospective review between January 2000 and December 2016. Thirty-three patients (median age 2y/o [0-38], weighing 9.6 kg [2.2-91]) underwent 39 transcatheter interventions on 39 lesions, mainly left main coronary (16/39; 39%) and right coronary (9/39; 23%) arteries...
May 9, 2018: Pediatric Cardiology
https://www.readbyqxmd.com/read/29741141/cardiac-arrest-in-the-catheterization-laboratory
#9
Kapil Yadav, Huu Tam Truong
Cardiac arrest in the Catheterization Lab is a rare and unique scenario that is often logistically challenging. It often has dire prognosis especially in patients suffering from severe pre-existing illnesses (high risk patient) such as acute myocardial infarction with cardiogenic shock, or patients undergoing high risk procedures. As the number of complex interventional procedures increases, cardiac arrest in the cath lab will become more common and optimal management of this scenario is critical for both the patient and operator...
May 9, 2018: Current Cardiology Reviews
https://www.readbyqxmd.com/read/29729613/effect-of-lateral-wedge-length-on-ambulatory-knee-kinetics
#10
Arielle G Fischer, Baptiste Ulrich, Laurent Hoffmann, Brigitte M Jolles, Julien Favre
BACKGROUND: Lateral wedge insoles (LWI) were proposed to treat medial knee osteoarthritis through reductions of the ambulatory knee adduction moment (KAM). Limited attention was however paid to the LWI length, resulting in unclear understanding of its effect on KAM reductions. The knee flexion moment (KFM) was also shown to be important in knee osteoarthritis, but little is known about the effect of LWI length on it. RESEARCH QUESTION: This study aimed to compare the KAM and KFM of healthy subjects walking with four different lengths of LWI, explicitly without LWI and with LWI below the hindfoot (HF), below the hindfoot and forefoot (HF + FF) and below the hindfoot, forefoot and hallux (HF + FF + HX) segments...
April 27, 2018: Gait & Posture
https://www.readbyqxmd.com/read/29721914/echocardiography-in-arterial-hypertension
#11
REVIEW
Giovanni de Simone, Costantino Mancusi, Roberta Esposito, Nicola De Luca, Maurizio Galderisi
Hypertension is a condition characterized by pressure and/or volume overloads and echocardiography is helpful and feasible to understand hemodynamic mechanisms. Echocardiographic information is sometimes critical and susceptible of modifying decision making. In this review, we provide detailed descriptions of the parameters that can be derived from a standard transthoracic echocardiogram, including some more recent techniques. We will also explain how each parameter might have impact in the evaluation of the hypertensive patient and give indications on when to refer patients to echo-labs, which parameters are critical and which ones might be redundant, and how to use the information obtained in the report...
May 2, 2018: High Blood Pressure & Cardiovascular Prevention: the Official Journal of the Italian Society of Hypertension
https://www.readbyqxmd.com/read/29705309/modeling-of-sonochemistry-in-water-in-the-presence-of-dissolved-carbon-dioxide
#12
Olivier Authier, Hind Ouhabaz, Stefano Bedogni
CO2 capture and utilization (CCU) is a process that captures CO2 emissions from sources such as fossil fuel power plants and reuses them so that they will not enter the atmosphere. Among the various ways of recycling CO2 , reduction reactions are extensively studied at lab-scale. However, CO2 reduction by standard methods is difficult. Sonochemistry may be used in CO2 gas mixtures bubbled through water subjected to ultrasound waves. Indeed, the sonochemical reduction of CO2 in water has been already investigated by some authors, showing that fuel species (CO and H2 ) are obtained in the final products...
July 2018: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/29704977/outcomes-of-an-innovative-evidence-based-practice-project-building-a-difficult-access-team-in-the-emergency-department
#13
Madeleine Whalen, Barbara Maliszewski, Rebecca Sheinfeld, Heather Gardner, Diana Baptiste
PROBLEM: Difficult venous access is a common problem in health care-especially in the emergency setting-that relies on quick diagnostics to differentiate patient acuities and administer critical medications. The creation of a dedicated team to address difficult venous access (DVA) is a possible solution to the problems of delayed venous access, yet no studies have been published on implementing such a team in the emergency department. METHODS: This was a quasi-experimental study in an urban emergency department...
April 25, 2018: Journal of Emergency Nursing: JEN: Official Publication of the Emergency Department Nurses Association
https://www.readbyqxmd.com/read/29689132/alcohol-delays-the-onset-of-puberty-in-the-female-rat-by-altering-key-hypothalamic-events
#14
Vinod K Srivastava, Jill K Hiney, William L Dees
BACKGROUND: Because alcohol (ALC) delays signs of pubertal development, we assessed the time course of events associated with the synthesis of critical hypothalamic peptides that regulate secretion of luteinizing hormone-releasing hormone (LHRH), the peptide that drives the pubertal process. METHODS: Immature female rats were administered either lab chow or Bio-Serve isocaloric control or ALC liquid diets from 27 through 33 days of age. On days 28, 29, 31 and 33 animals were killed by decapitation and tissue blocks containing the medial basal hypothalamus (MBH) and the rostral hypothalamic area (RHA) were isolated and stored frozen until assessed by Western blot analysis...
April 24, 2018: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/29688636/-critical-evaluation-of-the-ability-to-diagnose-periprosthetic-infection-in-a-medical-system-in-israel
#15
Raviv Allon, Nofar Ben Basat, Alexander Puhov, Yaron Zaulan, Ronit Wollstein
INTRODUCTION: Prosthetic joint infection is a dangerous complication of joint replacement arthroplasty. Besides its catastrophic consequences, differentiating a deep infection from other complications is not always trivial due to lack of clinical signs of infection, inaccurate tests, lab-dependent analysis, variable normal values and ongoing dispute in the literature regarding the recommended diagnosis algorithm. AIMS: The purpose of this study was to examine the diagnosis algorithm in our medical center...
April 2018: Harefuah
https://www.readbyqxmd.com/read/29674079/a-comparison-study-on-the-use-of-dowex-1-and-teva-resin-in-determination-of-99-tc-in-environmental-and-nuclear-coolant-samples-in-a-sia-system-with-icp-ms-detection
#16
Kamila Kołacińska, Zbigniew Samczyński, Jakub Dudek, Anna Bojanowska-Czajka, Marek Trojanowicz
This work refers to a comparative study of sorbents widely used in determinations of 99 Tc such as TEVA resin and Dowex 1. Despite having a similar functional group of quaternary amines, both materials represent different chromatographic methods-extraction (TEVA resin) and anion exchange (Dowex 1)-which provides a diverse range of their properties significant in determination of 99 Tc in flow conditions. The comparative tests, carried out in a SIA-LOV (Sequential Injection Analysis-Lab-on-Valve) system combined with mass spectrometric (ICP-MS) detection, considered several factors that are crucial from the standpoint of resin´s utility such as sorption capacity, durability, or selectivity, critical in 99 Tc separation from interferences...
July 1, 2018: Talanta
https://www.readbyqxmd.com/read/29674029/direct-immersion-single-drop-microextraction-and-in-drop-stirring-microextraction-for-the-determination-of-nanomolar-concentrations-of-lead-using-automated-lab-in-syringe-technique
#17
Ivana H Šrámková, Burkhard Horstkotte, Kateřina Fikarová, Hana Sklenářová, Petr Solich
Two operational modes for Lab-In-Syringe automation of direct-immersion single-drop microextraction have been developed and critically compared using lead in drinking water as the model analyte. Dithizone was used in the presence of masking additives as a sensitive chromogenic complexing reagent. The analytical procedure was carried out inside the void of an automatic syringe pump. Normal pump orientation was used to study extraction in a floating drop of a toluene-hexanol mixture. Placing the syringe upside-down allowed the use of a denser-than-water drop of chloroform for the extraction...
July 1, 2018: Talanta
https://www.readbyqxmd.com/read/29673624/an-efficient-method-to-control-high-mannose-and-core-fucose-levels-in-glycosylated-antibody-production-using-deoxymannojirimycin
#18
Sagit Shalel Levanon, Orit Aharonovitz, Ayelet Maor-Shoshani, Gita Abraham, Dan Kenett, Yehoshua Aloni
Glycosylation on the Fc region of recombinant Immunoglobulin G (IgG) therapeutic antibodies is a critical protein quality attribute which may affect the efficacy and safety of the molecule. During the development of biosimilar therapeutics, adjustment of the glycosylation profile is required in order to match the reference innovator profile. Deoxymannojirimycin (DMJ), a known inhibitor of mannosidase, was used in this study to modulate the glycosylation pattern of antibodies. The effect of DMJ, at concentrations of 5 μM - 500 μM, on non-fucosylated glycoform levels was tested in the biosynthesis processes of two different IgG1 (IgG1 #A and IgG1 #B) using two Chinese hamster ovary (CHO) cell lines (CHO-DXB-11 and CHOK1SV, respectively) in Erlenmeyer flasks and in lab scale bioreactors...
April 16, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29672232/a-multimethodological-study-of-preschoolers-preferences-for-aggressive-television-and-video-games
#19
Matthew R Jamnik, Lisabeth F DiLalla
The association between aggressive media and related behavior is complicated, and the role of underlying genetics has not been adequately explored. A better understanding of the role of genetics on the relationship between aggressive media and behavior, especially in young children, is critical. Using a twin/triplets sample (N = 184 children), the authors investigated the association between preschoolers' preferred media choices and their aggressive behaviors. A multimeasure methodology was utilized, examining children's reports of their preferred media games and shows, observed child negativity and aggression in the lab, and parent reports of their own and their children's aggressive behaviors...
April 19, 2018: Journal of Genetic Psychology
https://www.readbyqxmd.com/read/29669161/latest-advances-in-cardiac-valvular-replacement
#20
Ruggero De Paulis, Salvatore D'Aleo, Ilaria Chirichilli, Luca Paolo Weltert
The original monograph on valvular prostheses in Surgical Technology International was published in 1993. It represents a milestone and a reference point for critically organizing information on a complex and rapidly evolving topic. The last update was published in 2010. Since then, there have been significant developments regarding both "traditional" surgical prosthesis and valves for transcatheter implantation. Both bioprostheses and mechanical prostheses continue to evolve with respect to both their design and materials to further optimize hemodynamics and prevent a patient-prosthesis mismatch...
April 18, 2018: Surgical Technology International
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