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https://www.readbyqxmd.com/read/28931369/acute-hiv-infection-presenting-as-hemophagocytic-lymphohistiocytosis-case-report-and-review-of-the-literature
#1
Farheen Manji, Evan Wilson, Etienne Mahe, John Gill, John Conly
BACKGROUND: Hemophagocytic lymphohistiocytosis (HLH) is an uncommon systemic inflammatory condition that can result from infections, autoimmune diseases and malignancies. It is a rarely reported life threatening complication of an acute HIV infection, with only ten documented case reports per our literature search. We present a case of HLH secondary to acute HIV infection with a negative HIV antibody-based assay and high plasma viral load. CASE PRESENTATION: A 45 year old male with a past medical history of well controlled hypertension presented with fever, dizziness and non-bloody diarrhea...
September 20, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28929011/in-rats-fed-high-energy-diets-taste-rather-than-fat-content-is-the-key-factor-increasing-food-intake-a-comparison-of-a-cafeteria-and-a-lipid-supplemented-standard-diet
#2
Laia Oliva, Tània Aranda, Giada Caviola, Anna Fernández-Bernal, Marià Alemany, José Antonio Fernández-López, Xavier Remesar
BACKGROUND: Food selection and ingestion both in humans and rodents, often is a critical factor in determining excess energy intake and its related disorders. METHODS: Two different concepts of high-fat diets were tested for their obesogenic effects in rats; in both cases, lipids constituted about 40% of their energy intake. The main difference with controls fed standard lab chow, was, precisely, the lipid content. Cafeteria diets (K) were self-selected diets devised to be desirable to the rats, mainly because of its diverse mix of tastes, particularly salty and sweet...
2017: PeerJ
https://www.readbyqxmd.com/read/28924725/hiv-viral-suppression-37-states-and-the-district-of-columbia-2014
#3
Kristen L Hess, H Irene Hall
Achieving viral suppression among HIV-positive persons is a critical component of HIV treatment and prevention, because it leads to improved health outcomes for the individual and reduced risk of HIV transmission. There is wide variation in viral suppression across jurisdictions, races/ethnicities, age groups, and transmission risk groups. This analysis uses HIV surveillance data to examine rates of viral suppression among people living with diagnosed HIV (PLWDH) in 38 jurisdictions with complete lab reporting...
September 18, 2017: Journal of Community Health
https://www.readbyqxmd.com/read/28924562/assessment-of-analytical-performance-of-glucose-meter-in-pediatric-age-group-at-tertiary-care-referral-hospital
#4
Mohit Vijay Rojekar, Vandana Kumawat, Jayesh Panot, Surekha Khedkar, Arati Adhe-Rojekar
BACKGROUND: Glucometers are the excellent tools for self-monitoring of blood glucose (SMBG). They are important especially in the circumstances where continuous monitoring is mandatory and at decision making levels. Tight glycemic control protocols are important for preventing the ill effects of fluctuating glucose levels. This increases the use of glucometers in various healthcare settings. As technology advances, glucometers are getting better in terms of quality of results. But still some lacunae are there...
2017: Journal of Diabetes and Metabolic Disorders
https://www.readbyqxmd.com/read/28919476/current-status-of-the-pilot-scale-anaerobic-membrane-bioreactor-treatments-of-domestic-wastewaters-a-critical-review
#5
REVIEW
Chungheon Shin, Jaeho Bae
This review presented the performances of the pilot-scale anaerobic membrane bioreactors (AnMBRs) treating domestic wastewater. High COD removal efficiencies and low biosolids productions were achieved at HRTs comparable to conventional aerobic processes under ambient temperatures. The energy demands for fouling control in the pilot-scale AnMBRs ranged from 0.04 to 1.35kWh/m(3), which is lower than those of lab-scale AnMBRs and aerobic MBRs. The energy demands for fouling control were in the order of gas sparging>particle sparging>rotating membrane AnMBR...
September 5, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28919404/emergency-in-the-clinic-a-simulation-curriculum-to-improve-outpatient-safety
#6
Eve Espey, Gillian Baty, John Rask, Michelle Chungtuyco, Brenda Pereda, Lawrence Leeman
BACKGROUND: Emergency response skills are essential when events such as seizure, anaphylaxis or hemorrhage occur in the outpatient setting. As services and procedures increasingly move outside the hospital, training to manage complications may improve outcomes. OBJECTIVE: The objective of this study was to evaluate a simulation-based curriculum in outpatient emergency management skills with the outcome measures of graded objective performance and learner self-efficacy...
September 14, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28918967/accuracy-of-bedside-point-of-care-testing-in-critical-emergency-department-patients
#7
Braden W McIntosh, Jerina Vasek, Maria Taylor, Deborah Le Blanc, Henry C Thode, Adam J Singer
BACKGROUND: Point-of-care (POC) testing reduces laboratory turn-around having the potential to improve timely diagnosis and management. We compared the accuracy of nurse performed POC and core laboratory testing and determined whether deviations between the two were clinically meaningful. METHODS: We performed a prospective, observational study on a convenience sample of 50 critical care ED patients in whom a POC chemistry and hematocrit was ordered. Blood samples were divided into 2 aliquots; one sample was tested by the treating nurse using a handheld POC device and the other sample was tested in the core laboratory...
September 14, 2017: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28916246/pre-clinical-toxicology-considerations-for-vaccine-development
#8
REVIEW
Nabil Al-Humadi
Vaccine development requires pre-clinical toxicology studies, following good laboratory practice (GLP), before first in human (phase I) use. Many factors are critical in the final outcome of any pre-clinical toxicology study. The study design is one of these critical factors and should be carefully planned to avoid any false negative and/or false positive results. Preparation is another most critical factor in a successful study. Major changes in any procedure during the course of study should be avoided by all means...
September 12, 2017: Vaccine
https://www.readbyqxmd.com/read/28912156/neutrophils-are-critical-for-myelin-removal-in-a-peripheral-nerve-injury-model-of-wallerian-degeneration
#9
Jane A Lindborg, Matthias Mack, Richard E Zigmond
Wallerian degeneration (WD) is considered an essential preparatory stage to the process of axonal regeneration. In the peripheral nervous system, infiltrating monocyte-derived macrophages that utilize the chemokine receptor CCR2 to gain entry to injured tissues from the bloodstream, are purportedly necessary for efficient WD. However, our lab has previously reported that myelin clearance in the injured sciatic nerve proceeds unhindered in the Ccr2(-/-) mouse model. Here, we extensively characterize WD in male Ccr2(-/-) mice and identify a compensatory mechanism of WD that is facilitated primarily by neutrophils...
September 14, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28904485/temporary-left-ventricular-pacing-a-desperate-life-saving-measure-in-emergency-situation
#10
Ajaz Ahamad Lone, Mohd Iqbal Dar, Fayaz Ahamad Rather, Mohd Sultan Alai, Imran Hafiz, Jahangir Rashid Beigh
Transcutaneous or transvenous pacing of the right ventricle is performed as a routine practice for patients received with symptomatic bradycardia or complete heart block with relative ease in cath lab. However, more and more patients are received with multiple comorbidities, critical condition, and difficult vascular access. In this article, we describe a patient with difficult venous access with tricuspid regurgitation and displaced the right ventricular pacemaker temporary lead undergoing coronary angiography who was managed with emergent nonconventional left ventricular pacing...
August 2017: Indian Journal of Critical Care Medicine
https://www.readbyqxmd.com/read/28903896/the-regulatory-functions-of-pirna-piwi-in-spermatogenesis
#11
Zhi-Heng Yuan, Yan-Mei Zhao
A class of 24-32 nt PIWI-binding small noncoding RNAs (sncRNAs) termed as PIWI-interacting RNAs (piRNAs) have been identified in animal germline. Recent studies suggest that piRNA/PIWI pathway plays a critical role in both silencing of transposons and posttranscriptional regulation of mRNAs in animal germline. A study from Dr. Mofang Liu's lab in Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, reveals the physiological and pathological importance of PIWI metabolism (mouse PIWI as known as MIWI; human PIWI as HIWI) in mammalian spermatogenesis...
August 20, 2017: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/28871543/respiration-traits-as-novel-markers-for-plant-robustness-under-the-threat-of-climate-change-a-protocol-for-validation
#12
Birgit Arnholdt-Schmitt
Respiration traits allow calculating temperature-dependent carbon use efficiency and prediction of growth rates. This protocol aims (1) to enable validation of respiration traits as non-DNA biomarkers for breeding on robust plants in support of sustainable and healthy plant production; (2) to provide an efficient, novel way to identify and predict functionality of DNA-based markers (genes, polymorphisms, edited genes, transgenes, genomes, and hologenomes), and (3) to directly help farmers select robust material appropriate for a specified region...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28861134/introducing-mammalian-cell-culture-and-cell-viability-techniques-in-the-undergraduate-biology-laboratory
#13
Kristen Bowey-Dellinger, Luke Dixon, Kristin Ackerman, Cynthia Vigueira, Yewseok K Suh, Todd Lyda, Kelli Sapp, Michael Grider, Dinene Crater, Travis Russell, Michael Elias, V McNeil Coffield, Verónica A Segarra
Undergraduate students learn about mammalian cell culture applications in introductory biology courses. However, laboratory modules are rarely designed to provide hands-on experience with mammalian cells or teach cell culture techniques, such as trypsinization and cell counting. Students are more likely to learn about cell culture using bacteria or yeast, as they are typically easier to grow, culture, and manipulate given the equipment, tools, and environment of most undergraduate biology laboratories. In contrast, the utilization of mammalian cells requires a dedicated biological safety cabinet and rigorous antiseptic techniques...
2017: Journal of Microbiology & Biology Education: JMBE
https://www.readbyqxmd.com/read/28861133/microbial-murders-crime-scene-investigation-an-active-team-based-learning-project-that-enhances-student-enthusiasm-and-comprehension-of-clinical-microbial-pathogens
#14
J Jordan Steel
Microbial disease knowledge is a critical component of microbiology courses and is beneficial for many students' future careers. Microbiology courses traditionally cover core concepts through lectures and labs, but specific instruction on microbial diseases varies greatly depending on the instructor and course. A common project involves students researching and presenting a disease to the class. This method alone is not very effective, and course evaluations have consistently indicated that students felt they lacked adequate disease knowledge; therefore, a more hands-on and interactive disease project was developed called Microbial Murders...
2017: Journal of Microbiology & Biology Education: JMBE
https://www.readbyqxmd.com/read/28858763/electrochemical-biosensors-for-salmonella-state-of-the-art-and-challenges-in-food-safety-assessment
#15
REVIEW
Nádia F D Silva, Júlia M C S Magalhães, Cristina Freire, Cristina Delerue-Matos
According to the recent statistics, Salmonella is still an important public health issue in the whole world. Legislated reference methods, based on counting plate methods, are sensitive enough but are inadequate as an effective emergency response tool, and are far from a rapid device, simple to use out of lab. An overview of the commercially available rapid methods for Salmonella detection is provided along with a critical discussion of their limitations, benefits and potential use in a real context. The distinguished potentialities of electrochemical biosensors for the development of rapid devices are highlighted...
August 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28830772/covalent-and-non-covalent-chemical-engineering-of-actin-for-biotechnological-applications
#16
REVIEW
Saroj Kumar, Alf Mansson
The cytoskeletal filaments are self-assembled protein polymers with 8-25nm diameters and up to several tens of micrometres length. They have a range of pivotal roles in eukaryotic cells, including transportation of intracellular cargoes (primarily microtubules with dynein and kinesin motors) and cell motility (primarily actin and myosin) where muscle contraction is one example. For two decades, the cytoskeletal filaments and their associated motor systems have been explored for nanotechnological applications including miniaturized sensor systems and lab-on-a-chip devices...
August 19, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28830087/polysaccharide-production-by-lactic-acid-bacteria-from-genes-to-industrial-applications
#17
Ahmad A Zeidan, Vera Kuzina Poulsen, Thomas Janzen, Patrizia Buldo, Patrick M F Derkx, Gunnar Øregaard, Ana Rute Neves
The ability to produce polysaccharides with diverse biological functions is widespread in bacteria. In lactic acid bacteria (LAB), production of polysaccharides has long been associated with the technological, functional and health-promoting benefits of these microorganisms. In particular, the capsular polysaccharides and exopolysaccharides have been implicated in modulation of the rheological properties of fermented products. For this reason, screening and selection of exocellular polysaccharide-producing LAB has been extensively carried out by academia and industry...
August 1, 2017: FEMS Microbiology Reviews
https://www.readbyqxmd.com/read/28821648/bilingual-language-switching-in-the-lab-vs-in-the-wild-the-spatio-temporal-dynamics-of-adaptive-language-control
#18
Esti Blanco-Elorrieta, Liina Pylkkänen
For a bilingual human, every utterance requires a choice about which language to use. This choice is commonly regarded as part of general executive control, engaging prefrontal and anterior cingulate cortices similarly to many types of effortful task-switching. However, while language control within artificial switching paradigms has been heavily studied, the neurobiology of natural switching within socially cued situations has not been characterized. Additionally, although theoretical models address how language control mechanisms adapt to the distinct demands of different interactional contexts, these predictions have not been empirically tested...
August 16, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28813857/kinematic-effects-of-inertia-and-friction-added-by-a-robotic-knee-exoskeleton-after-prolonged-walking
#19
C Shirota, M R Tucker, O Lambercy, R Gassert
The capabilities of robotic gait assistive devices are ever increasing; however, their adoption outside of the lab is still limited. A critical barrier for the functionality of these devices are the still unknown mechanical properties of the human leg during dynamic conditions such as walking. We built a robotic knee exoskeleton to address this problem. Here, we present the effects of our device on the walking pattern of four subjects. We assessed the effects after a short period of acclimation as well as after a 1...
July 2017: IEEE ... International Conference on Rehabilitation Robotics: [proceedings]
https://www.readbyqxmd.com/read/28797909/smelling-themselves-dogs-investigate-their-own-odours-longer-when-modified-in-an-olfactory-mirror-test
#20
Alexandra Horowitz
While domestic dogs, Canis familiaris, have been found to be skillful at social cognitive tasks and even some meta-cognitive tasks, they have not passed the test of mirror self-recognition (MSR). Acknowledging the motivational and sensory challenges that might hinder performance, even before the question of self-recognition is broached, this study creates and enacts a novel design extrapolated from the species' natural behaviour. Given dogs' use of olfactory signals in communication, this experiment presents dogs with various canisters for approach and investigation...
October 2017: Behavioural Processes
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