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https://www.readbyqxmd.com/read/29781423/in-vitro-ovicidal-and-larvicidal-activities-of-some-saponins-and-flavonoids-against-parasitic-nematodes-of-goats
#1
Acidália Carine Vieira Santos, Francianne Oliveira Santos, Hélimar Gonçalves Lima, Gisele Dias Da Silva, Rosangela Soares Uzêda, Êuder Reis Dias, Alexsandro Branco, Klauber Viana Cardoso, Jorge Mauricio David, Mariana Borges Botura, Silvia Lima Costa, Maria José Moreira Batatinha
This study assessed the anthelmintic activity of plant-derived compounds against gastrointestinal nematodes of goats using the egg hatch and larval motility assays. The compounds tested were saponins (digitonin and aescin) and their respective sapogenins (aglycones), hecogenin acetate and flavonoids (catechin, hesperidin, isocordoin and a mixture of isocordoin and cordoin). Additionally, cytotoxicity of active substances was analysed on Vero cell through 3-4,5-dimethylthiazol-2-yl,2,5diphenyltetrazolium bromide (MTT) and propidium iodide (PI) tests...
May 21, 2018: Parasitology
https://www.readbyqxmd.com/read/29780682/-nitraria-schoberi-l-hairy-root-culture-as-a-source-of-compounds-with-antiviral-activity-against-influenza-virus-subtypes-%C3%B0-h5n1-and-%C3%B0-h3n2
#2
Tatiana Zheleznichenko, Evgenii Banaev, Sergey Asbaganov, Maria Voronkova, Tatiana Kukushkina, Ekaterina Filippova, Natalia Mazurkova, Larisa Shishkina, Tatyana Novikova
The hairy root culture of halophyte Nitraria schoberi was obtained by transforming seedlings' primary leaves with a wild strain of Agrobacterium rhizogenes 15834 SWISS (transformation frequency was 78.7%). The roots' transgenic status was confirmed by PCR analysis using rolB -, rolC -, virC -, virD1 -specific primers. There was a significant increase in the biosynthesis of flavonoids, hydroxycinnamic acids, pectins, protopectins and saponins in hairy roots as compared to intact plant roots. The сatechin content in ethanol extracts of transformed roots' exceeded this factor 3...
June 2018: 3 Biotech
https://www.readbyqxmd.com/read/29775211/proanthocyanidins-will-they-effectively-restrain-conspicuous-bacterial-strains-devolving-on-urinary-tract-infection
#3
REVIEW
Venkataseshan Jagannathan, Pragasam Viswanathan
Struvite or infection stones are one of the major clinical burdens among urinary tract infection, which occur due to the interaction between microbes and urine mineral components. Numerous urinary tract infection (UTI) causing microbes regulate through biofilm formation for survival from host defense, it is often found difficult in its eradication with simple anti-microbial agents and also the chance of recurrence and resistance development is significantly high. Cranberry consumption and maintenance of urinary tract health have been supported by clinical, epidemiological, and mechanistic studies...
May 18, 2018: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/29772774/selected-plant-metabolites-involved-in-oxidation-reduction-processes-during-bud-dormancy-and-ontogenetic-development-in-sweet-cherry-buds-prunus-avium-l
#4
Susanne Baldermann, Thomas Homann, Susanne Neugart, Frank-M Chmielewski, Klaus-Peter Götz, Kristin Gödeke, Gerd Huschek, Getrud E Morlock, Harshadrai M Rawel
Many biochemical processes are involved in regulating the consecutive transition of different phases of dormancy in sweet cherry buds. An evaluation based on a metabolic approach has, as yet, only been partly addressed. The aim of this work, therefore, was to determine which plant metabolites could serve as biomarkers for the different transitions in sweet cherry buds. The focus here was on those metabolites involved in oxidation-reduction processes during bud dormancy, as determined by targeted and untargeted mass spectrometry-based methods...
May 17, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29763441/chemical-composition-of-barks-from-quercus-faginea-trees-and-characterization-of-their-lipophilic-and-polar-extracts
#5
Joana P A Ferreira, Isabel Miranda, Vicelina B Sousa, Helena Pereira
The bark from Quercus faginea mature trees from two sites was chemically characterized for the first time. The barks showed the following composition: ash 14.6%, total extractives 13.2%, suberin 2.9% and lignin 28.2%. The polysaccharides were composed mainly of glucose and xylose (50.3% and 35.1% of all monosaccharides respectively) with 4.8% of uronic acids. The suberin composition was: ω-hydroxyacids 46.3% of total compounds, ɑ,ω-alkanoic diacids 22.3%, alkanoic acids 5.9%, alkanols 6.7% and aromatics 6...
2018: PloS One
https://www.readbyqxmd.com/read/29759184/application-of-a-webcam-camera-as-a-cost-effective-sensor-with-image-processing-for-dual-electrochemical-colorimetric-detection-system
#6
Kajorngai Thajee, Pathinan Paengnakorn, Wasin Wongwilai, Kate Grudpan
A webcam camera as an alternative cost-effective colorimetric sensor for the simple dual electrochemical - colorimetric detection system is proposed. Performance test of the system was investigated through electrochemical and colorimetric behaviors of redox reactions of potassium ferrocyanide complex. Image processing with two color systems, RGB and HSV, was applied together with various electrochemical techniques. Color responses obtained from the webcam correlated with electrochemical signals in various electrochemical techniques...
August 1, 2018: Talanta
https://www.readbyqxmd.com/read/29759183/evaluation-of-thrombin-inhibitory-activity-of-catechins-by-online-capillary-electrophoresis-based-immobilized-enzyme-microreactor-and-molecular-docking
#7
Qiao-Qiao Li, Feng-Qing Yang, Yin-Zhen Wang, Zhao-Yu Wu, Zhi-Ning Xia, Hua Chen
An online capillary electrophoresis (CE)-based thrombin (THR) immobilized enzyme microreactor (IMER) method was established to screen THR inhibitors in this study. S-2366 was used as chromogenic substrate for determination of THR activity and other kinetic constants. After continuously run for 50 times, the prepared IMER could still remain 89% of the initial immobilized enzyme activity. The Michaelis-Menten constant (Km ) of immobilized THR was measured as 0.514 mmol/L and the half-maximal inhibitory concentration (IC50 ) and inhibition constant (Ki ) of argatroban on THR were determined as 78...
August 1, 2018: Talanta
https://www.readbyqxmd.com/read/29758984/the-inhibition-of-methylglyoxal-induced-histone-h1-n%C3%AE%C2%B5-carboxymethyllysine-formation-by-catechin
#8
Lijun Yang, Xinping Li, Zhaozhen Wu, Cuixia Feng, Tianyu Zhang, Shaohua Dai, Qiang Dong
Reactive dicarbonyl species (RCS) such as methylglyoxal (MGO) and glyoxal (GO) are common intermediates in protein damage, leading to the formation of advanced glycation end products (AGEs) through nonenzymatic glycation. (+)-Catechin, a natural plant extract from tea, has been evaluated for its ability in trapping GO and MGO. However, (+)-catechin also is reported to have both anti-oxidant ability and pro-oxidant properties. Till now, whether (+)-catechin can inhibit the formation and its mechanism in nucleoprotein nonenzymatic glycation is still unclear...
May 14, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29758009/a-review-on-the-weight-loss-effects-of-oxidized-tea-polyphenols
#9
REVIEW
Dylan O'Neill Rothenberg, Caibi Zhou, Lingyun Zhang
The mechanistic systems in the body through which tea causes weight loss are complex and multi-dimensional. Additionally, the bioactive components in tea such as catechins, caffeine, and products of tea polyphenol oxidation vary greatly from one major tea type to the next. Green tea has been the primary subject of consideration for investigation into the preventative health effects of tea because it contains the highest levels of phenolic compounds and retains the highest antioxidant capabilities of any major tea type...
May 14, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29749738/secondary-metabolite-accumulation-associates-with-ecological-succession-of-endophytic-fungi-in-cynomorium-songaricum-rupr
#10
Jin-Long Cui, Yan-Yan Zhang, Vinod Vijayakumar, Gang Zhang, Meng-Liang Wang, Jun-Hong Wang
Cynomorium songaricum Rupr. is a rare root-parasitic plant distributed in the desert ecosystem. Little is known about the role of endophytes in accumulation of metabolites in C. songaricum. Here, the correlationship between the seven active components i.e., total sugars, flavonoids, protocatechuic acid, catechins and tannins, gallic and ursolic acids and the endophytic fungi of C. songaricum were investigated, and their causal relationships are discussed further. The results showed that the accumulation of seven components and the assembly of endophytic fungi changed with different plant developmental stages...
May 11, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29748675/catechin-supplemented-in-a-fos-diet-induces-weight-loss-by-altering-cecal-microbiota-and-gene-expression-of-colonic-epithelial-cells
#11
Jianming Luo, Lulu Han, Liu Liu, Lijuan Gao, Bin Xue, Yong Wang, Shiyi Ou, Michael Miller, Xichun Peng
Our previous study showed that catechin controlled rats' body weights and changed gut microbiota composition when supplemented into a high-fructo-oligosaccharide (FOS) diet. This experiment is devised to further confirm the relationship between specific bacteria in the colon and body weight gain, and to investigate how specific bacteria impact body weight by changing the expression of colonic epithelial cells. Forty obese rats were divided into four groups: three catechin-supplemented groups with a high-FOS diet (100, 400, and 700 mg kg-1 d-1 catechin, orally administered) and one group with a high-FOS diet only...
May 11, 2018: Food & Function
https://www.readbyqxmd.com/read/29747456/intestinal-absorption-and-antioxidant-activity-of-grape-pomace-polyphenols
#12
Veronica Sanda Chedea, Laurentiu Mihai Palade, Daniela Eliza Marin, Rodica Stefania Pelmus, Mihaela Habeanu, Mircea Catalin Rotar, Mihail Alexandru Gras, Gina Cecilia Pistol, Ionelia Taranu
The absorption and antioxidant activity of polyphenols from grape pomace (GP) are important aspects of its valorization as a feed additive in the diet of weaned piglets. This study aimed to evaluate the presence of polyphenols from GP both in vitro in IPEC cells and in vivo in the duodenum and colon of piglets fed with diets containing or not 5% GP and also to compare and correlate the aspects of their in vitro and in vivo absorption. Total polyphenolic content (TPC) and antioxidant status (TAS, CAT, SOD and GPx enzyme activity, and lipid peroxidation-TBARS level) were assessed in duodenum and colon of piglets fed or not a diet with 5% GP...
May 9, 2018: Nutrients
https://www.readbyqxmd.com/read/29747413/binding-of-catechins-to-staphylococcal-enterotoxin-a
#13
Yuko Shimamura, Mio Utsumi, Chikako Hirai, Shogo Nakano, Sohei Ito, Ai Tsuji, Takeshi Ishii, Takahiro Hosoya, Toshiyuki Kan, Norio Ohashi, Shuichi Masuda
Staphylococcal enterotoxin A (SEA) is a toxin protein, and is the most common cause of staphylococcal food poisoning. Polyphenols, such as catechins, are known to interact with proteins. In this study, we investigated the binding of catechins to SEA using SPR (Biacore), Fourier transform infrared spectroscopy (FT-IR), isothermal titration calorimetry (ITC), and protein-ligand docking. We found that (−)-epigallocatechin gallate (EGCG) could strongly bind to SEA. According to thermodynamic parameters, a negative Δ G indicated that the interaction between EGCG and SEA was spontaneous, and the electrostatic force accompanied by hydrophobic binding forces may play a major role in the binding...
May 9, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29744232/investigation-of-interactions-between-thrombin-and-ten-phenolic-compounds-by-affinity-capillary-electrophoresis-and-molecular-docking
#14
Qiao-Qiao Li, Yu-Xiu Yang, Jing-Wen Qv, Guang Hu, Yuan-Jia Hu, Zhi-Ning Xia, Feng-Qing Yang
Thrombin plays a vital role in blood coagulation, which is a key process involved in thrombosis by promoting platelet aggregation and converting fibrinogen to form the fibrin clot. In the receptor concept, drugs produce their therapeutic effects via interactions with the targets. Therefore, investigation of interaction between thrombin and small molecules is important to find out the potential thrombin inhibitor. In this study, affinity capillary electrophoresis (ACE) and in silico molecular docking methods were developed to study the interaction between thrombin and ten phenolic compounds ( p -hydroxybenzoic acid, protocatechuic acid, vanillic acid, gallic acid, catechin, epicatechin, dihydroquercetin, naringenin, apigenin, and baicalein)...
2018: Journal of Analytical Methods in Chemistry
https://www.readbyqxmd.com/read/29742305/red-grape-vitis-vinifera-l-flavonoids-down-regulate-collagen-type-iii-expression-after-uv-a-in-primary-human-dermal-blood-endothelial-cells
#15
Serena Di Francesco, Monica Savio, Nora Bloise, Giovanni Borroni, Lucia Anna Stivala, Riccardo G Borroni
Red grape (Vitis vinifera L.) flavonoids including flavan-3-ols (e.g., catechin and epicatechin), flavonols (e.g., quercetin) and anthocyanins (e.g., malvidin) exert anti-inflammatory and antioxidant activities. In the skin they also have a photoprotective action, and their effects have been extensively investigated in keratinocytes, melanocytes and fibroblasts. Despite their known effects also on blood vasculature, little is known on their activities on human dermal blood endothelial cells (HDBECs), which are critically involved in skin homeostasis as well as in the pathogenesis of neoplastic and inflammatory skin diseases...
May 9, 2018: Experimental Dermatology
https://www.readbyqxmd.com/read/29740891/effects-of-gut-microbiota-and-time-of-treatment-on-tissue-levels-of-green-tea-polyphenols-in-mice
#16
Anna B Liu, Siyao Tao, Mao-Jung Lee, Qi Hu, Xiaofeng Meng, Yong Lin, Chung S Yang
The previous studies have shown that tea polyphenols are metabolized by gut microbiota. This study investigated the effect of gut microbiota on the bioavailability, tissue levels, and degradation of tea polyphenols. Mice were treated with antibiotics (ampicillin/sulfamethoxazole/trimethoprim) in drinking water and the control mice received water for 11 days, and they were given an AIN93M diet enriched with 0.32% of Polyphenon E. The levels of catechins and their metabolites (if present) in the serum, liver, urine, and fecal samples were determined by high-performance liquid chromatography...
May 8, 2018: BioFactors
https://www.readbyqxmd.com/read/29739608/phenolic-compounds-as-beneficial-phytochemicals-in-pomegranate-punica-granatum-l-peel-a-review
#17
Balwinder Singh, Jatinder Pal Singh, Amritpal Kaur, Narpinder Singh
Pomegranate peel (PoP), a juice byproduct often considered as a waste, comprises nearly around 30-40% portion of the fruit. Phenolic compounds (one class of bioactive phytochemicals) are primarily concentrated in the peel portion of pomegranate fruit. In PoP, the main phenolic compounds reported in the literature include flavonoids (anthocyanins such as pelargonidin, delphinidin, cyanidin along with their derivatives and anthoxanthins such as catechin, epicatechin and quercetin), tannins (ellagitannins and ellagic acid derivatives such as punicalagin, punicalin and pedunculagin) and phenolic acids (such as chlorogenic, caffeic, syringic, sinapic, p-coumaric, ferulic, ellagic, gallic and cinnamic acid)...
September 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29739591/in-vitro-health-promoting-properties-of-antioxidant-dietary-fiber-extracted-from-spent-coffee-coffee-arabica-l-grounds
#18
Kenia Vázquez-Sánchez, Nuria Martinez-Saez, Miguel Rebollo-Hernanz, Maria Dolores Del Castillo, Marcela Gaytán-Martínez, Rocio Campos-Vega
Antioxidant dietary fiber extracted from spent coffee grounds (FSCG) was evaluated as a potential functional food ingredient when incorporated in a food model (biscuits), and digested in vitro under simulated human gastrointestinal conditions. FSCG added to biscuits increased its total dietary fiber, antioxidant capacity after in vitro digestion, bioaccessibility of phenolic compounds (gallic acid and catechin) and amino acids. Furthermore, advanced glycation end products (AGEs), involved in chronic diseases, decreased up to 6-folds in the biscuits containing FSCG when compared with the traditional biscuit...
September 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29739590/quantification-of-ascorbyl-adducts-of-epigallocatechin-gallate-and-gallocatechin-gallate-in-bottled-tea-beverages
#19
Wei-Lun Hung, Siyu Wang, Shengmin Sang, Xiaochun Wan, Yu Wang, Chi-Tang Ho
Catechins are the major bioactive compounds existing in tea leaves (Camellia sinensis). Dehydroascorbic acid is (DHAA) a reactive dicarbonyl species and previous studies have demonstrated that catechins could effectively trap DHAA to form ascorbyl adducts of catechins, especially epigallocatechin gallate (EGCG). Since catechins in the aqueous solution are unstable due to their structural features, ascorbic acid (AA) is usually added to bottled tea beverages to protect catechins. However, whether ascorbyl adducts of catechins are formed in bottled tea beverages remains unclear...
September 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29735073/phenolic-compounds-are-highly-correlated-to-the-antioxidant-capacity-of-genotypes-of-oenocarpus-distichus-mart-fruits
#20
Sérgio Henrique Brabo de Sousa, Rafaella de Andrade Mattietto, Renan Campos Chisté, Ana Vânia Carvalho
This research aimed to evaluate 32 genotypes of Oenocarpus distichus fruits regarding the contents of total phenolic compounds, flavonoids, flavanols, monomeric anthocyanins, antioxidant capacity (ABTS and DPPH assays), and the phenolic compound profiles of the five genotypes that presented the highest yields of bioactive compounds. The genotypes were harvested in three different locations in Pará State, Northern Brazil, (Belém, São João do Araguaia and Marabá). Among the 32 genotypes, the highest bioactive compound contents and antioxidant capacity were found for three genotypes harvested in Belém (B-3, B-7 and B-8) and two harvested in São João do Araguaia (SJ-1 and SJ-4), and the total phenolic compounds varied from 131...
June 2018: Food Research International
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