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Elena García-Martín, María I Ramos, José A Cornejo-García, Segismundo Galván, James R Perkins, Laura Rodríguez-Santos, Hortensia Alonso-Navarro, Félix J Jiménez-Jiménez, José A G Agúndez
Background: The Gamma-aminobutyric acid type A receptor (GABA-A receptor) is affected by ethanol concentrations equivalent to those reached during social drinking. At these concentrations, ethanol usually causes impairment in reaction and motor times in most, but not all, individuals. Objectives: To study the effect of GABA-A receptor variability in motor and reaction times, and the effect of low ethanol doses. Methods: Two hundred and fifty healthy subjects received one single dose of 0.5 g/Kg ethanol per os ...
2018: Frontiers in Cellular Neuroscience
Hongyan Ren, Lijie Guan, Liansheng Zhao, Yin Lin, Yincheng Wang, Zhenxing Yang, Xuan Li, Xiaohong Ma, Xiongchao Cheng, Wenhao Deng, Katherine J Aitchison, Liping Cao, Tao Li
In this study, we investigated the association between bipolar I disorder (BDI) and between cognitive deficits therein and SNPs in GABAergic receptor genes. The sample comprised 477 patients with BDI and 438 healthy controls, with three neurocognitive tests being administered in 123 patients and 164 controls. For three SNPs, rs505474, rs1398175, and rs4868029 in the GABRA2, GABRA4, and GABRP genes, respectively, their allele frequencies were significantly different between patients and controls (Bonferroni-adjusted p = values 3...
November 14, 2017: American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics
Hye Youn Sung, San-Duk Yang, Woong Ju, Jung-Hyuck Ahn
Metastasis is a major cause of therapeutic failure in ovarian cancer. To elucidate molecular mechanisms of ovarian cancer metastasis, we previously established a metastatic xenograft mouse model using human ovarian carcinoma SK-OV-3 cells. Using gene expression profiling, we found that γ-aminobutyric acid (GABA)A receptor π subunit (GABRP) expression was upregulated (>4-fold) in metastatic tissues from our xenograft mice compared with SK-OV-3 cells. Importantly, GABRP knockdown diminished the migration and invasion of SK-OV-3 cells, and reduced extracellular signal-regulated kinase (ERK) activation while overexpression of GABRP exhibited significantly increased cell migration, invasion and ERK activation...
May 19, 2017: Experimental & Molecular Medicine
Alessio Soggiu, Cristian Piras, Viviana Greco, Paola Devoto, Andrea Urbani, Luigino Calzetta, Marco Bortolato, Paola Roncada
The enzyme 5α-reductase (5αR) catalyzes the conversion of progesterone and testosterone into neuroactive steroids implicated in a wide array of behavioral functions. The prototypical 5αR inhibitor, finasteride (FIN), is clinically approved for the treatment of androgenic alopecia and benign prostatic hyperplasia. Recent evidence has shown that FIN, albeit generally well tolerated, can induce untoward psychological effects in a subset of patients; furthermore, this drug may have therapeutic efficacy for a number of different neuropsychiatric conditions, ranging from Tourette syndrome to schizophrenia...
December 2016: Psychoneuroendocrinology
Jing Ma, Yan Zhang, Jun Wang, Tianyu Zhao, Ping Ji, Jinlin Song, Hongmei Zhang, Wenping Luo
Gamma-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the adult mammalian central nervous system, has been reported to play an important physiological role in peripheral non-neuronal tissues, such as tumors. However, whether deregulated GABA is associated with oral squamous cell carcinoma (OSCC) is currently unknown. In this study, we investigated the effects of GABA on the proliferation of the OSCC cell line, Tca8113. Immunohistochemical analyses were performed to examine the expression of GABA A type receptor pi subunit (GABRP) in human OSCC tissues, and reverse transcription polymerase chain reaction, immunofluorescence staining and western blot analysis were performed to examine the expression of GABRP in Tca8113 cells...
July 2016: International Journal of Molecular Medicine
Junjie Lu, Qian Zhang, Dongmei Tan, Wenping Luo, Hai Zhao, Jing Ma, Hao Liang, Yi Tan
Gamma-aminobutyric acid (GABA) functions primarily as an inhibitory neurotransmitter through its receptors in the mature central nervous system. The GABA type A receptor π subunit (GABRP) has been identified in the tissues of the reproductive system, particularly in the uterus. In addition, we have previously detected GABRP expression in both human and mouse placentas. To examine the role of GABRP in trophoblastic cell invasion, we constructed a pIRES2-GABRP-EGFP plasmid which was used for the transfection of a human placental cell line derived from first trimester extravillous trophoblasts (HTR-8/SVneo)...
July 2016: International Journal of Molecular Medicine
Hun-Soo Kim, Eun-Heui Jin, Ji-Su Mo, Hyeok Shim, Shin-Seok Lee, Soo-Cheon Chae
Gamma-aminobutyric acid receptor subunit pi (GABRP) is involved in inhibitory synaptic transmission in the central nervous system. This gene encodes multisubunit chloride channels and is also expressed in numerous nonneuronal tissues such as the uterus and the ovaries. This study was aimed to validate whether the polymorphisms in the GABRP gene are associated with the susceptibility to systematic lupus erythematosus (SLE). The genotype frequencies of the rs929763, rs732157, and rs3805455 of the GABRP gene in SLE patients were significantly different from those of the control group (P < 0...
2015: Journal of Immunology Research
Xiaguang Chen, Cunshuan Xu
OBJECTIVE: To investigate the transdifferentiation relationship between eight types of liver cell during rat liver regeneration (LR). MATERIALS AND METHODS: 114 healthy Sprague-Dawley (SD) rats were used in this experimental study. Eight types of liver cell were isolated and purified with percoll density gradient centrifugation and immunomagentic bead methods. Marker genes for eight types of cell were obtained by retrieving the relevant references and databases...
2015: Cell Journal
Pavol Zubor, Jozef Hatok, Petra Moricova, Karol Kajo, Ivana Kapustova, Andrea Mendelova, Peter Racay, Jan Danko
The gene expression profile of breast cancer has been described as a great breakthrough on the way to comprehend differences in cancer origin, behavior and therapy. However, gene expression profile in histologically normal epithelium (HNEpi) which could harbor genetic abnormalities predisposing breast tissue to develop malignancy was minor scope for scientists in the past. Thus, we aimed to analyze gene expressions in HNEpi and breast cancer tissue (BCTis) in order to establish its value as potential diagnostic marker for cancer development...
May 2015: Molecular Biology Reports
Pavol Zubor, Jozef Hatok, Petra Moricova, Ivana Kapustova, Karol Kajo, Andrea Mendelova, Monika Kmetova Sivonova, Jan Danko
Gene expression profile‑based taxonomy of breast cancer (BC) has been described as a significant breakthrough in comprehending the differences in the origin and behavior of cancer to allow individually tailored therapeutic approaches. In line with this, we hypothesized that the gene expression profile of histologically normal epithelium (HNEpi) could harbor certain genetic abnormalities predisposing breast tissue cells to develop human epidermal growth factor receptor 2 (HER2)‑positive BC. Thus, the aim of the present study was to assess gene expression in normal and BC tissue (BCTis) from patients with BC in order to establish its value as a potential diagnostic marker for cancer development...
February 2015: Molecular Medicine Reports
Gina M Sizemore, Steven T Sizemore, Darcie D Seachrist, Ruth A Keri
Breast cancer is a heterogeneous disease comprised of distinct subtypes predictive of patient outcome. Tumors of the basal-like subtype have a poor prognosis due to inherent aggressiveness and the lack of targeted therapeutics. Basal-like tumors typically lack estrogen receptor-α, progesterone receptor and HER2/ERBB2, or in other words they are triple negative (TN). Continued evaluation of basal-like breast cancer (BLBC) biology is essential to identify novel therapeutic targets. Expression of the pi subunit of the GABA(A) receptor (GABRP) is associated with the BLBC/TN subtype, and herein, we reveal its expression also correlates with metastases to the brain and poorer patient outcome...
August 29, 2014: Journal of Biological Chemistry
María Ángeles Castilla, María Ángeles López-García, María Reina Atienza, Juan Manuel Rosa-Rosa, Juan Díaz-Martín, María Luisa Pecero, Begoña Vieites, Laura Romero-Pérez, Javier Benítez, Annarica Calcabrini, José Palacios
Vestigial-like 1 (VGLL1) is a poorly characterized gene encoding a transcriptional co-activator structurally homologous to TAZ and YAP that modulates the Hippo pathway in Drosophila. In this study, we examined the expression of VGLL1 and its intronic miRNA, miR-934, in breast cancer. VGLL1 and miR-934 expression miRNA profiling was carried out on frozen samples of grade 3 invasive ductal carcinomas. VGLL1 protein was also examined in 433 sporadic and BRCA1-associated breast carcinomas on tissue microarrays...
August 2014: Endocrine-related Cancer
Arjun Guha, Michelle Vasconcelos, Rui Zhao, Adam C Gower, Jayaraj Rajagopal, Wellington V Cardoso
Clara cells (CCs) are a morphologically and operationally heterogeneous population of Secretoglobin Scgb1a1-expressing secretory cells that are crucial for airway homeostasis and post-injury repair. Analysis of the extent and origin of CC diversity are limited by knowledge of genes expressed in these cells and their precursors. To identify novel putative markers of CCs and explore the origins of CC diversity, we characterized global changes in gene expression in embryonic lungs in which CCs do not form due to conditional disruption of Notch signaling (Rbpjk(CNULL))...
2014: PloS One
Hřibová Petra, Honsová Eva, Brabcová Irena, Hrubá Petra, Viklický Ondřej
Both antibody mediated (AMR) and T-cell mediated (TCMR) rejections either acute or chronic represent the main reason for late graft dysfunction. In this study we aimed to evaluate differences in the intrarenal expression patterns of immune system related genes in acute and chronic rejections. Graft biopsies were performed and evaluated according to Banff classification. Using the TaqMan Low Density Array, the intrarenal expressions of 376 genes relating to immune response (B-cell activation, T-cell activation, chemokines, growth factors, immune regulators, and apoptosis) were analyzed in the four rejection categories: chronic AMR, chronic TCMR, acute AMR, and acute TCMR...
2013: Clinical & Developmental Immunology
Rongrong Zhao, Ruiling Zhang, Wenqiang Li, Yanhui Liao, Jinsong Tang, Qin Miao, Wei Hao
INTRODUCTION: Alcohol dependence is a complex disease caused by a confluence of environmental and genetic factors. Epigenetic mechanisms have been shown to play an important role in the pathogenesis of alcohol dependence. METHODS: To determine if alterations in gene-specific methylation were associated with alcohol dependence, a genome-wide DNA methylation analysis was performed on peripheral blood mononuclear cells from alcohol-dependent patients and siblings without alcohol dependence as controls...
March 2013: Asia-Pacific Psychiatry: Official Journal of the Pacific Rim College of Psychiatrists
Sarah A Andres, Guy N Brock, James L Wittliff
BACKGROUND: Genomics provides opportunities to develop precise tests for diagnostics, therapy selection and monitoring. From analyses of our studies and those of published results, 32 candidate genes were identified, whose expression appears related to clinical outcome of breast cancer. Expression of these genes was validated by qPCR and correlated with clinical follow-up to identify a gene subset for development of a prognostic test. METHODS: RNA was isolated from 225 frozen invasive ductal carcinomas,and qRT-PCR was performed...
2013: BMC Cancer
Mengjia Pu, Zhijun Zhang, Zhi Xu, Yanyan Shi, Leiyu Geng, Yonggui Yuan, Xiangrong Zhang, Gavin P Reynolds
AIM: To investigate the role of genetic polymorphisms in glutamatergic and GABAergic genes and their interactions with environmental stressors in antidepressant response. METHODS: A set of 114 SNPs of 34 glutamatergic and GABAergic genes, mainly in promoter and coding regions, were genotyped in 281 Chinese Han major depressive disorder patients. The 17-item Hamilton Depression Rating Scale was used to evaluate the symptom severity and therapeutic efficacy. Childhood Trauma Questionnaire and Life Events Scale were used for assessing early-onset and recent stressful life events, respectively...
February 2013: Pharmacogenomics
Wenping Luo, Zhenzhen Liu, Dongmei Tan, Qian Zhang, Hongying Peng, Yingxiong Wang, Yi Tan
Uterine decidualization, characterized by stromal cell proliferation and differentiation into polyploid decidual cells, is critical to the establishment of pregnancy in mice, although the mechanism underlying this process remains poorly understood. This study is the first to investigate the expression of gamma-amino butyric acid (GABA) and the GABA A-type receptor π subunit (GABPR) in the early-pregnancy mouse uterus and their roles in decidualization. The expression of GABRP was detected from Day 4 to 8 of pregnancy...
January 2013: Molecular Reproduction and Development
Enrique Baca-Garcia, Concepción Vaquero-Lorenzo, M Mercedes Perez-Rodriguez, Mònica Gratacòs, Mònica Bayés, Ricardo Santiago-Mozos, Jose Miguel Leiva-Murillo, Mario de Prado-Cumplido, Antonio Artes-Rodriguez, Antonio Ceverino, Carmen Diaz-Sastre, Pablo Fernandez-Navarro, Javier Costas, José Fernandez-Piqueras, Montserrat Diaz-Hernandez, Jose de Leon, Enrique Baca-Baldomero, Jeronimo Saiz-Ruiz, J John Mann, Ramin V Parsey, Angel Carracedo, Xavier Estivill, Maria A Oquendo
Despite marked morbidity and mortality associated with suicidal behavior, accurate identification of individuals at risk remains elusive. The goal of this study is to identify a model based on single nucleotide polymorphisms (SNPs) that discriminates between suicide attempters and non-attempters using data mining strategies. We examined functional SNPs (n = 840) of 312 brain function and development genes using data mining techniques. Two hundred seventy-seven male psychiatric patients aged 18 years or older were recruited at a University hospital psychiatric emergency room or psychiatric short stay unit...
January 5, 2010: American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics
Undine E Lang, Imke Puls, Daniel J Muller, Nathalie Strutz-Seebohm, Jurgen Gallinat
Schizophrenia is a complex disorder, where family, twin and adoption studies have been demonstrating a high heritability of the disease and that this disease is not simply defined by several major genes but rather evolves from addition or potentiation of a specific cluster of genes, which subsequently determines the genetic vulnerability of an individual. Linkage and association studies suggest that a genetic vulnerablility, is not forcefully leading to the disease since triggering factors and environmental influences, i...
2007: Cellular Physiology and Biochemistry
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