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https://www.readbyqxmd.com/read/29780175/rapid-identification-of-a-cooling-tower-associated-legionnaires-disease-outbreak-supported-by-polymerase-chain-reaction-testing-of-environmental-samples-new-york-city-2014-2015
#1
Isaac Benowitz, Robert Fitzhenry, Christopher Boyd, Michelle Dickinson, Michael Levy, Ying Lin, Elizabeth Nazarian, Belinda Ostrowsky, Teresa Passaretti, Jennifer Rakeman, Amy Saylors, Elena Shamoonian, Terry-Ann Smith, Sharon Balter
We investigated an outbreak of eight Legionnaires' disease cases among persons living in an urban residential community of 60,000 people. Possible environmental sources included two active cooling towers (air-conditioning units for large buildings) <1 km from patient residences, a market misting system, a community-wide water system used for heating and cooling, and potable water. To support a timely public health response, we used real-time polymerase chain reaction (PCR) to identify Legionella DNA in environmental samples within hours of specimen collection...
April 2018: Journal of Environmental Health
https://www.readbyqxmd.com/read/29779890/high-throughput-screening-enhances-kidney-organoid-differentiation-from-human-pluripotent-stem-cells-and-enables-automated-multidimensional-phenotyping
#2
Stefan M Czerniecki, Nelly M Cruz, Jennifer L Harder, Rajasree Menon, James Annis, Edgar A Otto, Ramila E Gulieva, Laura V Islas, Yong Kyun Kim, Linh M Tran, Timothy J Martins, Jeffrey W Pippin, Hongxia Fu, Matthias Kretzler, Stuart J Shankland, Jonathan Himmelfarb, Randall T Moon, Neal Paragas, Benjamin S Freedman
Organoids derived from human pluripotent stem cells are a potentially powerful tool for high-throughput screening (HTS), but the complexity of organoid cultures poses a significant challenge for miniaturization and automation. Here, we present a fully automated, HTS-compatible platform for enhanced differentiation and phenotyping of human kidney organoids. The entire 21-day protocol, from plating to differentiation to analysis, can be performed automatically by liquid-handling robots, or alternatively by manual pipetting...
May 15, 2018: Cell Stem Cell
https://www.readbyqxmd.com/read/29776031/regularized-lattice-boltzmann-model-for-immiscible-two-phase-flows-with-power-law-rheology
#3
Yan Ba, Ningning Wang, Haihu Liu, Qiang Li, Guoqiang He
In this work, a regularized lattice Boltzmann color-gradient model is developed for the simulation of immiscible two-phase flows with power-law rheology. This model is as simple as the Bhatnagar-Gross-Krook (BGK) color-gradient model except that an additional regularization step is introduced prior to the collision step. In the regularization step, the pseudo-inverse method is adopted as an alternative solution for the nonequilibrium part of the total distribution function, and it can be easily extended to other discrete velocity models no matter whether a forcing term is considered or not...
March 2018: Physical Review. E
https://www.readbyqxmd.com/read/29774899/smartphone-based-rapid-quantification-of-viable-bacteria-by-single-cell-microdroplet-turbidity-imaging
#4
Xiaonan Cui, Lihui Ren, Yufei Shan, Xixian Wang, Zhenlong Yang, Chunyu Li, Jian Xu, Bo Ma
Standard plate count (SPC) has been recognized as the golden standard for the quantification of viable bacteria. However, SPC usually takes one to several days to grow individual cells into a visible colony, which greatly hampers its application in rapid bacteria enumeration. Here we present a microdroplet turbidity imaging based digital standard plate count (dSPC) method to overcome this hurdle. Instead of cultivating on agar plates, bacteria are encapsulated in monodisperse microdroplets for single-cell cultivation...
May 18, 2018: Analyst
https://www.readbyqxmd.com/read/29769587/explorations-on-the-ecological-role-of-toxin-secretion-and-delivery-in-jawless-predatory-polychaeta
#5
N Cuevas, M Martins, A P Rodrigo, C Martins, P M Costa
Motivated by biotechnological prospects, there is increasing evidence that we may just be scraping the tip of the iceberg of poisonous marine invertebrates, among which the Polychaeta are promising candidates for bioprospecting. Here we show that an inconspicuous phyllodocid uses toxins in its uncanny feeding strategy. The worm, a jawless active predator characterised by its bright green colour, preys on larger invertebrates (including conspecifics) by extracting tissue portions with its powerful proboscis through suction...
May 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29764717/mitochondrial-damage-and-cytoskeleton-reorganization-in-human-dermal-fibroblasts-exposed-to-artificial-visible-light-similar-to-screen-emitted-light
#6
Adeline Rascalou, Jérôme Lamartine, Pauline Poydenot, Frédéric Demarne, Nicolas Bechetoille
BACKGROUND: Artificial visible light is everywhere in modern life. Social communication confronts us with screens of all kinds, and their use is on the rise. We are therefore increasingly exposed to artificial visible light, the effects of which on skin are poorly known. OBJECTIVE: The purpose of this study was to model the artificial visible light emitted by electronic devices and assess its effect on normal human fibroblasts. METHODS: The spectral irradiance emitted by electronic devices was optically measured and equipment was developed to accurately reproduce such artificial visible light...
May 5, 2018: Journal of Dermatological Science
https://www.readbyqxmd.com/read/29761864/high-throughput-detection-and-screening-of-plantsmodified-by-geneediting-using-quantitative-real-time-pcr
#7
Cheng Peng, Hua Wang, Xiaoli Xu, Xiaofu Wang, Xiaoyun Chen, Wei Wei, Yongmin Lai, Guoquan Liu, Godwin Ian, Jieqin Li, Ling Zhang, Junfeng Xu
Gene editing techniques are becoming powerful tools for modifying target genes in organisms. Although several methods have been developed to detect gene-edited organisms, these techniques are time- and labour-intensive. Meanwhile, few studies have investigated high-throughput detection and screening strategies for plants modified by gene editing. In this study, we developed a simple, sensitive and high-throughput quantitative real-time(qPCR)-based method. The qPCR-based methodexploits two differently labelled probes that are placed within one amplicon at the gene editing target site to simultaneously detect the wild type and a gene-edited mutant...
May 15, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29756447/electrochemical-methodologies-for-the-detection-of-pathogens
#8
Mandana Amiri, Abolfazl Bezaatpour, Hamed Jafari, Rabah Boukherroub, Sabine Szunerits
Bacterial infections remain of the principal causes of morbidity and mortality worldwide. The number of death due to infections is declining every year by only 1% with a forecast of 13 million deaths in 2050. Among the 1400 recognized human pathogens, the majority of infectious diseases is caused by just a few, about 20 pathogens only. While the development of vaccinations and novel anti-bacterial drugs and treatments are at the forefront of research, and strongly financially supported by policy makers, another manner to limit and control infectious outbreaks is targeting the development and implementation of early warning systems, which indicate qualitatively and quantitatively the presence of a pathogen...
May 14, 2018: ACS Sensors
https://www.readbyqxmd.com/read/29752245/modeling-and-characterization-of-capacitive-elements-with-tissue-as-dielectric-material-for-wireless-powering-of-neural-implants
#9
Reza Erfani, Fatemeh Marefat, Amir M Sodagar, Pedram Mohseni
This paper reports on the modeling and characterization of capacitive elements with tissue as the dielectric material, representing the core building block of a capacitive link for wireless power transfer to neural implants. Each capacitive element consists of two parallel plates that are aligned around the tissue layer and incorporate a grounded, guarded, capacitive pad to mitigate the adverse effect of stray capacitances and shield the plates from external interfering electric fields. The plates are also coated with a biocompatible, insulating, coating layer on the inner side of each plate in contact with the tissue...
May 2018: IEEE Transactions on Neural Systems and Rehabilitation Engineering
https://www.readbyqxmd.com/read/29746794/an-accurate-estimation-of-the-horizontal-acceleration-of-a-rower-s-centre-of-mass-using-inertial-sensors-a-validation
#10
Lotte L Lintmeijer, Gert S Faber, Hessel R Kruk, A J Knoek van Soest, Mathijs J Hofmijster
For a valid determination of a rower's mechanical power output, the anterior-posterior (AP) acceleration of a rower's centre of mass (CoM) is required. The current study was designed to evaluate the accuracy of the determination of this acceleration using a full-body inertial measurement units (IMUs) suit in combination with a mass distribution model. Three methods were evaluated. In the first two methods, IMU data were combined with either a subject-specific mass distribution or a standard mass distribution model for athletes...
May 10, 2018: European Journal of Sport Science
https://www.readbyqxmd.com/read/29746537/kinetic-and-kinematic-variables-affecting-trunk-flexion-during-level-walking-in-patients-with-lumbar-spinal-stenosis
#11
Tatsuya Igawa, Junji Katsuhira, Akira Hosaka, Kenta Uchikoshi, Shinichi Ishihara, Ko Matsudaira
Lumbar spinal stenosis causes cauda equina and nerve root compression, resulting in neurological symptoms. Although trunk flexion during level walking may alleviate these symptoms by enabling spinal canal decompression, some patients do not use this strategy. We aimed to identify the kinetic and kinematic variables that affect trunk flexion in patients during level walking. Gait was recorded in 111 patients using a three-dimensional motion capture system and six force plates. From the data recorded, walking velocity, bilateral step length, cycle time, maximum trunk flexion angle, forward pelvic tilt angle, pelvic rotation angle, maximum and minimum joint angles, and moment and power of the lower limb were calculated...
2018: PloS One
https://www.readbyqxmd.com/read/29738917/analysis-and-comparison-of-focused-ion-beam-milling-and-vibratory-polishing-sample-surface-preparation-methods-for-porosity-study-of-u-mo-plate-fuel-for-research-and-test-reactors
#12
Bjorn Westman, Brandon Miller, Jan-Fong Jue, Assel Aitkaliyeva, Dennis Keiser, James Madden, Julie D Tucker
Uranium-Molybdenum (U-Mo) low enriched uranium (LEU) fuels are a promising candidate for the replacement of high enriched uranium (HEU) fuels currently in use in a high power research and test reactors around the world. Contemporary U-Mo fuel sample preparation uses focused ion beam (FIB) methods for analysis of fission gas porosity. However, FIB possess several drawbacks, including reduced area of analysis, curtaining effects, and increased FIB operation time and cost. Vibratory polishing is a well understood method for preparing large sample surfaces with very high surface quality...
May 2, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29734911/three-year-review-of-dorsal-plating-for-complex-intra-articular-fractures-of-the-distal-radius
#13
Michelle Spiteri, Wayne Ng, Jamie Matthews, Dominic Power, Mark Brewster
BACKGROUND: The volar approach is commonly used for plating intra-articular fractures of the distal radius. Despite this, certain fracture configurations are more suitable for dorsal plate fixation. This technique has not gained favour due to the reported high incidence of extensor tendon irritation and attrition ruptures. With the advent of lower profile plates this risk has decreased. METHODS: We report on forty-six cases performed in a tertiary hand centre between January 2011 and May 2014...
June 2018: Journal of Hand Surgery Asian-Pacific Volume
https://www.readbyqxmd.com/read/29734124/ankle-joint-function-during-walking-in-tophaceous-gout-a-biomechanical-gait-analysis-study
#14
Matthew Carroll, Mark Boocock, Nicola Dalbeth, Sarah Stewart, Christopher Frampton, Keith Rome
BACKGROUND: The foot and ankle are frequently affected in tophaceous gout, yet kinematic and kinetic changes in this region during gait are unknown. The aim of the study was to evaluate ankle biomechanical characteristics in people with tophaceous gout using three-dimensional gait analysis. METHODS: Twenty-four participants with tophaceous gout were compared with 24 age-and sex-matched control participants. A 9-camera motion analysis system and two floor-mounted force plates were used to calculate kinematic and kinetic parameters...
April 17, 2018: Gait & Posture
https://www.readbyqxmd.com/read/29716596/assessment-of-static-posturography-and-pedobarography-for-the-detection-of-unilateral-forelimb-lameness-in-ponies
#15
Lidia Pitti, Maarten Oosterlinck, Maria L Díaz-Bertrana, José M Carrillo, Mónica Rubio, Joaquin Sopena, Angelo Santana, José M Vilar
BACKGROUND: Static posturography and pedobarography are based on the detection of postural imbalance and, consequently, the pressure redistribution between limbs in lame subjects. These techniques have proven to be useful for the detection of lameness in humans and dogs. The main objective of this study was to test the suitability of static posturography and pedobarography in diagnosing lameness in ponies. A pressure platform was used to obtain postural data (statokinesiograms, mean X and Y, length, LFS ratio, and mean velocity) from 10 sound ponies and 7 ponies with unilateral forelimb lameness...
May 2, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29716368/absolute-ion-detection-efficiencies-of-microchannel-plates-and-funnel-microchannel-plates-for-multi-coincidence-detection
#16
K Fehre, D Trojanowskaja, J Gatzke, M Kunitski, F Trinter, S Zeller, L Ph H Schmidt, J Stohner, R Berger, A Czasch, O Jagutzki, T Jahnke, R Dörner, M S Schöffler
Modern momentum imaging techniques allow for the investigation of complex molecules in the gas phase by detection of several fragment ions in coincidence. For these studies, it is of great importance that the single-particle detection efficiency ε is as high as possible, as the overall efficiency scales with εn , i.e., the power of the number of detected particles. Here we present measured absolute detection efficiencies for protons of several micro-channel plates (MCPs), including efficiency enhanced "funnel MCPs...
April 2018: Review of Scientific Instruments
https://www.readbyqxmd.com/read/29715854/sub-4-fs-laser-pulses-at-high-average-power-and-high-repetition-rate-from-an-all-solid-state-setup
#17
Chih-Hsuan Lu, Tobias Witting, Anton Husakou, Marc J J Vrakking, A H Kung, Federico J Furch
The generation of high average power, carrier-envelope phase (CEP) stable, near-single-cycle pulses at a repetition rate of 100 kHz is demonstrated using an all solid-state setup. By exploiting self-phase modulation in thin quartz plates and air, the spectrum of intense pulses from a high-power, high repetition rate non-collinear optical parametric chirped pulse amplifier (NOPCPA) is extended to beyond one octave, and pulse compression down to 3.7 fs is achieved. The octave-spanning spectrum furthermore allows performing straightforward f-to-2f interferometry by frequency-doubling the long-wavelength part of the spectrum...
April 2, 2018: Optics Express
https://www.readbyqxmd.com/read/29712566/powiff-prospective-study-of-wrist-internal-fixation-of-fracture-a-protocol-for-a-single-centre-superiority-randomised-controlled-trial-to-study-the-efficacy-of-the-vrp-2-0-distal-radius-plate-austofix-versus-the-va-lcp-depuy-synthes-for-distal-radius-fractures
#18
V D Varghese, Peter Smitham, Stuart Howell, Suzanne Edwards, Mark Rickman
BACKGROUND: Distal radial fractures are one of the most common orthopaedic cases that present to the A&E department. Surgical intervention is warranted in displaced intraarticular fractures and fractures with more than the recommended angulation or shortening, and is most commonly treated with volarly placed fixed angle locking plates. The aim of this study is to determine and compare the efficacy of two different plates for surgical treatment of distal radius fractures. The VRP 2...
April 30, 2018: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/29709875/a-variable-frequency-bidirectional-shear-horizontal-sh-wave-transducer-based-on-dual-face-shear-d-24-piezoelectric-wafers
#19
Hongchen Miao, Qiang Huan, Faxin Li, Guozheng Kang
Focusing the incident wave beam along a given direction is very useful in guided wave based structural health monitoring (SHM), as it will not only save input power but also simplify the interpretation of signals. Although the fundamental shear horizontal (SH0 ) wave is of practical importance in SHM due to its non-dispersive characteristics so far there have been very limited transducers which can control the radiation patterns of SH0 wave. In this work, a variable-frequency bidirectional SH0 wave piezoelectric transducer (BSH-PT) is proposed, which consists of two rectangular face-shear (d24 ) PZT wafers...
April 24, 2018: Ultrasonics
https://www.readbyqxmd.com/read/29692955/the-reliability-of-vertical-jump-tests-between-the-vertec-and-my-jump-phone-application
#20
Vanessa R Yingling, Dimitri A Castro, Justin T Duong, Fiorella J Malpartida, Justin R Usher, Jenny O
Background: The vertical jump is used to estimate sports performance capabilities and physical fitness in children, elderly, non-athletic and injured individuals. Different jump techniques and measurement tools are available to assess vertical jump height and peak power; however, their use is limited by access to laboratory settings, excessive cost and/or time constraints thus making these tools oftentimes unsuitable for field assessment. A popular field test uses the Vertec and the Sargent vertical jump with countermovement; however, new low cost, easy to use tools are becoming available, including the My Jump iOS mobile application (app)...
2018: PeerJ
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