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Dan-Dan Wang, Qing-Xi Wu, Wen-Juan Pan, Sajid Hussain, Shomaila Mehmood, Yan Chen
Background: Polysaccharides extracted from fungus that have been used widely in the food and drugs industries due to biological activities. Objective: The objective of the present study was to investigate the tumor-suppressive activity and mechanism of a novel polysaccharide (SAP) extracted from Sarcodon aspratus . Methods: The SAP was extracted and purified using Sepharose CL-4B gel from S. aspratus . The cytotoxicity of SAP on cell lines was determined by MTT method...
2018: Food & Nutrition Research
Dustin A Bruening, Kota Z Takahashi
BACKGROUND: Kinematic multi-segment foot models have been increasingly used to study foot function. The addition of kinetics to these models may enhance their utility; however, this been hindered by limitations in measuring ground reaction forces (GRFs) under individual foot segments. PURPOSE: To determine the accuracy of partitioning segment GRFs from a single force platform on foot joint kinetics. METHODS: Two potential partitioning methods were applied to a previously published three-segment kinetic foot model...
March 6, 2018: Gait & Posture
Darika Saitawee, Aroon Teerakapong, Noppawan Phumala Morales, Paiboon Jitprasertwong, Doosadee Hormdee
BACKGROUND AND OBJECTIVE: Curcumin, one of an established curcuminoid substances extracted from Curcuma longa, has been used as a photosensitizer (PS) in photodynamic therapy (PDT). Curcuminoid substances has been reported to have benefits in treating dental chronic infection and inflammation diseases, such as chronic periodontitis. The purpose of this study was to find the optimum concentration of Curcuma longa (CL) extract, containing all curcuminoid substances, and the power density of blue light (BL) in photodynamic therapy against periodontally pathogenic bacteria, A...
March 10, 2018: Photodiagnosis and Photodynamic Therapy
Hongcai Gao, Leigang Xue, Sen Xin, John B Goodenough
A safe potassium rechargeable battery of high energy density and excellent cycling stability is demonstrated. The cell contained a polyaniline cathode into which the anion component of the electrolyte salt is inserted on charge, extracted on discharge; but the K+ ion of a KPF6 salt is plated/stripped on the potassium-metal anode. The use of a p-type polymer cathode increases the cell voltage. By replacing the organic-liquid electrolyte in a glass-fiber separator by a polymer-gel electrolyte of cross-linked poly(methyl methacrylate), a dendrite-free potassium anode can be plated/stripped and the electrode/electrolyte interface stabilized...
March 13, 2018: Angewandte Chemie
Maryam Maleki, Samaneh Badri, Hamed Shayestehepour, Mokhtar Arazpour, Farzam Farahmand, Mohamad Ebrahim Mousavi, Ehsan Abdolahi, Hasan Farkhondeh, John S Head, Navid Golchin, Mohammad Ali Mardani
BACKGROUND: The aim of this study was to assess the performance of an original powered foot clearance creator (PFCC) mechanism worn in conjunction with an isocentric reciprocal gait orthosis (IRGO) and evaluate its effect on trunk compensatory movements and spatiotemporal parameters in nine healthy subjects. METHOD: A PFCC motorized mechanism was designed that incorporated twin sole plates, the movements of which enabled increased toe to floor clearance during swing phase...
March 12, 2018: Disability and Rehabilitation. Assistive Technology
Hongze Wang, Motoki Nakanishi, Yosuke Kawahito
An experimental system to observe the inner phenomenon of keyhole and molten pool through glass plate by high speed camera and spectrometer in high power density laser welding was set up. Two circular flows in the molten pool were observed by high speed camera, which transferred the mass from the front to the rear of the keyhole to keep the mass balance. Temperature distribution in the molten pool was firstly detected by spectrometer, which indicated that the circular flows acted as the cooling system to take heat away from the keyhole...
March 5, 2018: Optics Express
Sandro Bartolomei, Federico Nigro, Sandro Ruggeri, Ivan Malagoli Lanzoni, Simone Ciacci, Franco Merni, Eliahu Sadres, Jay R Hoffman, Gabriele Semprini
The purpose of the present study was to validate the ballistic push-up test performed with hands on a force plate (BPU) as a method to measure upper-body power. Twenty-eight experienced resistance trained men (age = 25.4 ± 5.2 y; body mass = 78.5 ± 9.0 kg; body height = 179.6 ± 7.8 cm) performed, two days apart, a bench press 1RM test and upper-body power tests. Mean power and peak power were assessed using the bench press throw test (BT) and the BPU test performed in randomized order. The area under the force/power curve (AUC) obtained at BT was also calculated...
March 6, 2018: Journal of Strength and Conditioning Research
Núria Daranas, Anna Bonaterra, Jesús Francés, Jordi Cabrefiga, Emilio Montesinos, Esther Badosa
A viability qPCR (v-qPCR) assay was developed for the unambiguous detection and quantification of Lactobacillus plantarum PM411 viable cells in aerial plant surfaces. A 972 bp region of a PM411 predicted prophage with mosaic architecture enabled the identification of a PM411 strain-specific molecular marker. Three primer sets, with different amplicon lengths (92, 188, and 317 bp), and one TaqMan probe were designed. All the qPCR assays showed good linearity over a 4-log range and good efficiencies, but differed in sensitivity...
March 9, 2018: Applied and Environmental Microbiology
Qiang Qiu, James D P Moore, Sylvain Barbot, Lujia Feng, Emma M Hill
Understanding the rheological properties of the upper mantle is essential to develop a consistent model of mantle dynamics and plate tectonics. However, the spatial distribution and temporal evolution of these properties remain unclear. Here, we infer the rheological properties of the asthenosphere across multiple great megathrust earthquakes between 2004 and 2014 along the Sumatran subduction zone, taking advantage of decade-long continuous GPS and tide-gauge measurements. We observe transient mantle wedge flow following these earthquakes, and infer the temporal evolution of the effective viscosity...
March 8, 2018: Nature Communications
Catarina Moreirinha, Nádia Osório, Carla Pereira, Sara Simões, Ivonne Delgadillo, Adelaide Almeida
The occurrence of infections by pathogenic bacteria is one of the main sources of financial loss for the aquaculture industry. This problem often cannot be solved with antibiotic treatment or vaccination. Phage therapy seems to be an alternative environmentally-friendly strategy to control infections. Recognizing the cellular modifications that bacteriophage therapy may cause to the host is essential in order to confirm microbial inactivation, while understanding the mechanisms that drive the development of phage-resistant strains...
March 8, 2018: Antibiotics
Ting Huang, Zaichun Liu, Yunlong Li, Yanqiu Li, Long Chao, Chao Chen, Yueming Tan, Qingji Xie, Shouzhuo Yao, Yuping Wu
Poly(5-hydroxytryptamine) (poly(5-HT)) is exploited as a new and efficient enzyme-immobilization matrix for amperometric and biofuel cell (BFC)-based biosensing. A GOx-poly(5-HT)-Pd nanoparticles (PdNPs) bionanocomposite is prepared by Na2 PdCl4 -initiated oxidized polymerization of 5-hydroxytryptamine (5-HT) in a neutral aqueous solution containing glucose oxidase (GOx), and this bionanocomposite and then chitosan (CS) are cast-coated on a Pd-plated Au electrode to yield a CS/GOx-poly(5-HT)-PdNPs/Pdplate /Au enzyme electrode...
July 12, 2018: Analytica Chimica Acta
Soram Oh, Su-Mi Shin, Hyun-Jung Kim, Janghyun Paek, Sung-Joon Kim, Tai Hyun Yoon, Sun-Young Kim
The purpose of this study was to assess the influence of the types and thicknesses of glass ceramic plates on light transmittance and compare the degrees of conversion (DC) of resin cement under the ceramic materials. Three ceramic plates with thicknesses of 0.5, 1.0, 2.0, and 4.0 mm were fabricated from each of five commercial ceramic blocks in shade A2: high-translucency and low-translucency IPS Empress CAD (Emp_HT and Emp_LT); high-translucency and low-translucency IPS e.max CAD (Emx_HT and Emx_LT); and Vita Mark II (Vita)...
March 2, 2018: International Journal of Oral Science
A Jonas, T Meurer, B Kanngießer, I Mantouvalou
The resolving power and relative efficiency of two off-axis reflection zone plates (RZPs) in the soft X-ray range between 1 nm and 5 nm were investigated. RZPs focus only a very narrow bandwidth around the design wavelength. By misaligning the RZP, the focused wavelength can be tuned through a much wider spectral range. Using a laser-produced plasma source, we demonstrate that a single RZP can be efficiently used for spectroscopy at arbitrary wavelengths in the investigated soft X-ray range.
February 2018: Review of Scientific Instruments
Akiyoshi Hirayama, Hiroshi Abe, Nozomi Yamaguchi, Sho Tabata, Masaru Tomita, Tomoyoshi Soga
A sheath-flow interface is the most common ionization technique in CE-ESI-MS. However, this interface dilutes the analytes with the sheath liquid and decreases the sensitivity. In this study, we developed a sheathless CE-MS interface to improve sensitivity. The interface was fabricated by making small crack approximately 2 cm from the end of a capillary column fixed on a plastic plate, and then covering the crack with a dialysis membrane to prevent metabolite loss during separation. A voltage for CE separation was applied between the capillary inlet and the buffer reservoir...
March 1, 2018: Electrophoresis
Yingji Jin, Seung Cheol Kim, Hyoung Jin Kim, Woong Ju, Yun Hwan Kim, Hong-Jin Kim
There has been no attempt to apply protein-based markers of exfoliated cervical cells (ECCs) for primary screening of cervical cancer. In the present study, the levels of six tumor-associated protein [TAPs: Sialyl Lewis A (SLeA), Cancer Antigen 15-3 (CA 15-3), p53, heat shock protein (Hsp)70, Hsp27 and squamous cervical carcinoma antigen (SCCA)]and of two human papillomavirus (HPV) viral proteins (HPV16 E7 and HPV16 L1) of ECCs lysates were evaluated by enzyme-linked immunosorbent assays (ELISAs).The wells of 96-well plates were coated with the ECCs lysates from normal, cervical intraepithelial neoplasia (CIN) I, CIN II, CIN III and cancer groups, and candidate proteins were detected by relevant antibodies...
February 28, 2018: Archives of Pharmacal Research
Toby Edwards, Tania Spiteri, Benjamin Piggott, Joshua Bonhotal, G Gregory Haff, Christopher Joyce
The purpose of this study was to establish the reliability and sensitivity of a countermovement jump (CMJ) and perceptual wellness questionnaire in NCAA Division I men's basketball players. Twelve male collegiate basketball athletes completed 3 maximal CMJ's on a portable force plate and a customized perceptual wellness questionnaire prior to training on non-consecutive days. CMJ variables were derived from computer software and used in analysis. All CMJ variables expressed acceptable trial-to-trial reliability (CV < 8%) except eccentric duration (CV = 8...
February 27, 2018: Journal of Strength and Conditioning Research
Wei Li, Xiaoxue Xu, Wenjiang Li, Yun Zhao, Minfang Chen
A novel, cost-effective biomedical triboelectric nanogenerator (TENG) has been fabricated based on biocompatible medical 317L stainless steel (317L SS) and polylactic acid (PLLA) films as the friction layers, whose surface roughness designed by simple and low-cost laser etching and hot-embossing template methods, respectively. With the increase of the TENG's tribo-interface roughness and the molecular weight of the PLLA, the power output of TENG was greatly increased owing to more charges produced and excellent mechanical properties of PLLA possessed...
February 27, 2018: Nanotechnology
Wei Yu, Zheng Duan, Guang Zhang, Changhong Liu, Shoushan Fan
Carbon nanotubes (CNTs) and other related CNT-based materials with high thermal conductivity can be used as promising heat dissipation materials. Meanwhile, the miniaturization and high functionality of portable electronics, such as laptops and mobile phones, are achieved at the cost of overheating the high power-density components. The heat removal for hot spots occurring in a relatively narrow space requires simple and effective cooling methods. Here, an auxiliary passive cooling approach by the aid of a flat plate (aluminum-magnesium alloy) is investigated to accommodate heat dissipation in a narrow space...
February 26, 2018: Nano Letters
Kenneth E McCarson, Michelle K Winter, Dale R Abrahamson, Nancy E Berman, Peter G Smith
Behavioral phenotyping is a crucial step in validating animal models of human disease. Most traditional behavioral analyses rely on investigator observation of animal subjects, which can be confounded by inter-observer variability, scoring consistency, and the ability to observe extremely rapid, small, or repetitive movements. Force-Plate Actimeter (FPA)-based assessments can quantify locomotor activity and detailed motor activity with an incredibly rich data stream that can reveal details of movement unobservable by the naked eye...
February 21, 2018: Neurobiology of Learning and Memory
Virginia E Glazier, Thomas Murante, Kristy Koselny, Daniel Murante, Marisol Esqueda, Gina A Wall, Melanie Wellington, Chiung-Yu Hung, Anuj Kumar, Damian J Krysan
Genetic interaction analysis is a powerful approach to the study of complex biological processes that are dependent on multiple genes. Because of the largely diploid nature of the human fungal pathogen Candida albicans , genetic interaction analysis has been limited to a small number of large-scale screens and a handful for gene-by-gene studies. Complex haploinsufficiency, which occurs when a strain containing two heterozygous mutations at distinct loci shows a phenotype that is distinct from either of the corresponding single heterozygous mutants, is an expedient approach to genetic interactions analysis in diploid organisms...
February 22, 2018: G3: Genes—Genomes—Genetics
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