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https://www.readbyqxmd.com/read/29149992/a-separable-surface-enhanced-raman-scattering-substrate-modified-with-mil-101-for-detection-of-overlapping-and-invisible-compounds-after-thin-layer-chromatography-development
#1
Bin Bin Zhang, Yi Shi, Hui Chen, Qing Xia Zhu, Feng Lu, Ying Wei Li
By coupling surface-enhanced Raman spectroscopy (SERS) with thin-layer chromatography (TLC), a powerful method for detecting complex samples was successfully developed. However, in the TLC-SERS method, metal nanoparticles serving as the SERS-active substrate are likely to disturb the detection of target compounds, particularly in overlapping compounds after TLC development. In addition, the SERS detection of compounds that are invisible under both visible light and UV254/365 after TLC development is still a significant challenge...
January 2, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29140977/low-cost-and-open-source-multi-fluorescence-imaging-system-for-teaching-and-research-in-biology-and-bioengineering
#2
Isaac Nuñez, Tamara Matute, Roberto Herrera, Juan Keymer, Timothy Marzullo, Timothy Rudge, Fernán Federici
The advent of easy-to-use open source microcontrollers, off-the-shelf electronics and customizable manufacturing technologies has facilitated the development of inexpensive scientific devices and laboratory equipment. In this study, we describe an imaging system that integrates low-cost and open-source hardware, software and genetic resources. The multi-fluorescence imaging system consists of readily available 470 nm LEDs, a Raspberry Pi camera and a set of filters made with low cost acrylics. This device allows imaging in scales ranging from single colonies to entire plates...
2017: PloS One
https://www.readbyqxmd.com/read/29137740/morphology-dependent-sensing-performance-of-dihydro-tetrazine-functionalized-mof-toward-al-iii
#3
Sayed Ali Akbar Razavi, Mohammad Yaser Masoomi, Ali Morsali
A pillared MOF, [Zn(OBA)(H2DPT)0.5].DMF (TMU-34), based on dihydro tetrazine functionalized pillar spacer (H2DPT=3,6-di(pyridin-4-yl)-1,4-dihydro-1,2,4,5-tetrazine) and V-shape dicarboxylate linker (H2OBA=4,4'-oxybis(benzoic acid)) was synthesized by reflux and ultrasonic methods. The effects of sonication time, initial concentration of reagents and sonication power on size and morphology have been optimized. This MOF has been characterized by scanning electron microscopy, FT-IR spectra, X-ray powder diffraction and N2 adsorption at 77K...
March 2018: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/29136892/green-synthesis-of-monolithic-column-incorporated-with-graphene-oxide-using-room-temperature-ionic-liquid-and-eutectic-solvents-for-capillary-electrochromatography
#4
Xin-Xin Li, Li-Shun Zhang, Chao Wang, Yan-Ping Huang, Zhao-Sheng Liu
In this work, a hybrid monolith incorporated with graphene oxide (GO) was prepared in the first time with binary green porogens of deep eutectic solvents (DESs) and room temperature ionic liquids (RTILs). GO was modified with 3-(trimethoxysilyl) propylmethacrylate (γ-MPS), and the resultant GO-MPS can be incorporated into poly (methacrylic acid-co-butylmethacrylate-co-ethylene glycol dimethacrylate) monoliths covalently. A hybrid monolithic column with high permeability and homogeneity can be achieved due to good dispersion of GO-MPS in the green solvents...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29135470/planar-screening-by-charge-polydisperse-counterions
#5
Martin Trulsson, Emmanuel Trizac, Ladislav Samaj
We study how a neutralising cloud of counterions screens the electric field of a uniformly charged planar membrane (plate), when the counterions are characterised by a distribution of charges (or valence), n(q). We work out analytically the one-plate and two-plate cases, at the level of non-linear Poisson-Boltzmann theory. The (essentially asymptotic) predictions are successfully compared to numerical solutions of the full Poisson-Boltzmann theory, but also to Monte Carlo simulations...
November 14, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/29129132/reliability-of-force-velocity-relationships-during-deadlift-high-pull
#6
Wei Lu, Sébastien Boyas, Marc Jubeau, Abderrahmane Rahmani
This study aimed to evaluate the within- and between-session reliability of force, velocity and power performances and to assess the force-velocity relationship during the deadlift high pull (DHP). Nine participants performed two identical sessions of DHP with loads ranging from 30 to 70% of body mass. The force was measured by a force plate under the participants' feet. The velocity of the 'body + lifted mass' system was calculated by integrating the acceleration and the power was calculated as the product of force and velocity...
November 13, 2017: Sports Biomechanics
https://www.readbyqxmd.com/read/29127057/quantitative-analysis-of-clonidine-and-ephedrine-by-a-microfluidic-system-on-chip-electromembrane-extraction-followed-by-high-performance-liquid-chromatography
#7
Mahroo Baharfar, Yadollah Yamini, Shahram Seidi, Monireh Karami
In this work, a microfluidic device was developed for on-chip electromembrane extraction of trace amounts of ephedrine (EPH) and clonidine (CLO) in human urine and plasma samples followed by HPLC-UV analysis. Two polymethylmethacrylate plates were used as substrates and a microchannel was carved in each plate. The microchannel channel on the underneath plate provided the flow pass of the sample solution and the one on the upper plate dedicated to a compartment for the stagnant acceptor phase. A piece of polypropylene sheet was impregnated by an organic solvent and mounted between the two parts of the chip device...
November 2, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29126605/a-novel-patterned-magnetic-micropillar-array-substrate-for-analysis-of-cellular-mechanical-responses
#8
Kazuaki Nagayama, Takuya Inoue, Yasuhiro Hamada, Takeo Matsumoto
Traction forces generated at cellular focal adhesions (FAs) play an essential role in regulating various cellular functions. These forces (1-100 nN) can be measured by observing the local displacement of a flexible substrate upon which cells have been plated. Approaches employing this method include using microfabricated arrays of poly(dimethylsiloxane) (PDMS) micropillars that bend by cellular traction forces. A tool capable of applying a force to FAs independently, by actively moving the micropillars, should become a powerful tool to delineate the cellular mechanotransduction mechanisms...
October 25, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/29116118/phononic-crystal-waveguide-transducers-for-nonlinear-elastic-wave-sensing
#9
Francesco Ciampa, Akash Mankar, Andrea Marini
Second harmonic generation is one of the most sensitive and reliable nonlinear elastic signatures for micro-damage assessment. However, its detection requires powerful amplification systems generating fictitious harmonics that are difficult to discern from pure nonlinear elastic effects. Current state-of-the-art nonlinear ultrasonic methods still involve impractical solutions such as cumbersome signal calibration processes and substantial modifications of the test component in order to create material-based tunable harmonic filters...
November 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29112910/biomechanical-characterization-of-ex-vivo-human-brain-using-ultrasound-shear-wave-spectroscopy
#10
Emmanuel Nicolas, Samuel Callé, Stéphane Nicolle, David Mitton, Jean-Pierre Remenieras
The characterization of brain tissue is crucial to better understand neurological disorders. Mechanical characterization is an emerging tool in that field. The purpose of this work was to validate a transient ultrasound technique aimed at measuring dispersion of mechanical parameters of the brain tissue. The first part of this work was dedicated to the validation of that technique by comparing it with two proven rheology methods: a rotating plate rheometer, and a viscoelastic spectroscopy apparatus. Experiments were done on tissue mimicking gels...
November 4, 2017: Ultrasonics
https://www.readbyqxmd.com/read/29107205/photodynamic-damage-predominates-on-different-targets-depending-on-cell-growth-phase-of-candida-albicans
#11
Alessandra Baptista, Caetano P Sabino, Silvia C Núñez, Walter Miyakawa, Airton A Martin, Martha S Ribeiro
Photodynamic inactivation (PDI) has been reported to be effective to eradicate a wide variety of pathogens, including antimicrobial-resistant microorganisms. The aim of this study was to identify the potential molecular targets of PDI depending on growth phase of Candida albicans. Fungal cells in lag (6h) and stationary (48h) phases were submitted to PDI mediated by methylene blue (MB) combined with a (662±21) nm-LED, at 360mW of optical power. Pre-irradiation time was 10min and exposure times were 12min, 15min and 18min delivering radiant exposures of 129...
October 10, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29102856/validity-and-reliability-of-an-iphone-app-to-assess-time-velocity-and-leg-power-during-a-sit-to-stand-functional-performance-test
#12
Juan Diego Ruiz-Cárdenas, Juan José Rodríguez-Juan, Rowan R Smart, Jennifer M Jakobi, Gareth R Jones
The purposes of this study were: (i) Analyze the concurrent validity and reliability of an iPhone App for measuring time, velocity and power during a single sit-to-stand (STS) test compared with measurements recorded from a force plate; and (ii) Evaluate the relationship between the iPhone App measures with age and functional performance. Forty-eight healthy individuals (age range: 26-81 years) were recruited. All participants completed a STS test on a force plate with the movement recorded on an iPhone 6 at 240 frames-per-second...
October 31, 2017: Gait & Posture
https://www.readbyqxmd.com/read/29097687/genome-scale-single-cell-mechanical-phenotyping-reveals-disease-related-genes-involved-in-mitotic-rounding
#13
Yusuke Toyoda, Cedric J Cattin, Martin P Stewart, Ina Poser, Mirko Theis, Teymuras V Kurzchalia, Frank Buchholz, Anthony A Hyman, Daniel J Müller
To divide, most animal cells drastically change shape and round up against extracellular confinement. Mitotic cells facilitate this process by generating intracellular pressure, which the contractile actomyosin cortex directs into shape. Here, we introduce a genome-scale microcantilever- and RNAi-based approach to phenotype the contribution of > 1000 genes to the rounding of single mitotic cells against confinement. Our screen analyzes the rounding force, pressure and volume of mitotic cells and localizes selected proteins...
November 2, 2017: Nature Communications
https://www.readbyqxmd.com/read/29093652/semi-functional-quantitative-flow-cytometry-assay-for-lymphocytic-choriomeningitis-virus-titration
#14
Young Ho Ban, Sang-Jun Ha
Quantitative PCR and plaque assay are powerful virological techniques used to measure the load of defective or infectious virus in mouse and human. However, these methods display limitations such as cross contamination and long run-time. Here, we describe a novel technique termed as semi-functional quantitative flow cytometry (SFQF) for the accurate estimation of the quantity of infectious lymphocytic choriomeningitis virus (LCMV). LCMV titration method using flow cytometry was previously developed but has technical shortcomings, owing to the less optimized parameters such as cell overgrowth, plate scale, and detection threshold...
October 2017: Immune Network
https://www.readbyqxmd.com/read/29092530/x-ray-spectrometer-having-12-000-resolving-power-at-8-kev-energy
#15
John F Seely, Lawrence T Hudson, Albert Henins, Uri Feldman
An x-ray spectrometer employing a thin (50 μm) silicon transmission crystal was used to record high-resolution Cu Kα spectra from a laboratory x-ray source. The diffraction was from the (331) planes that were at an angle of 13.26° to the crystal surface. The components of the spectral lines resulting from single-vacancy (1s) and double-vacancy (1s and 3d) transitions were observed. After accounting for the natural lifetime widths from reference double-crystal spectra and the spatial resolution of the image plate detector, the intrinsic broadening of the transmission crystal was measured to be as small as 0...
October 2017: Review of Scientific Instruments
https://www.readbyqxmd.com/read/29091044/dispersion-risk-associated-with-surgical-debridement-devices
#16
Mark Granick, Liel Rubinsky, Chaitra Parthiban, Mayilvahanan Shanmugam, Narayanan Ramasubbu
INTRODUCTION: Surgical instrumentation is now available to facilitate wound debridement. The 2 primary options involve different energy applications, but both have the potential to spray. The Versajet II (Smith & Nephew, London, UK) utilizes a high-powered water jet to disrupt tissue and remove debris by means of the Venturi effect. The SonicVac (Misonix, Farmingdale, NY) is a direct-contact, low-frequency ultrasound debriding device. It delivers a high-energy ultrasound to a wound surface via a fluid medium, causing bubble cavitation, a physical effect of rapid pressure waves causing bubbles to form and implode that releases mechanical energy...
October 2017: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/29089109/explosive-lower-limb-extension-mechanics-an-on-land-vs-in-water-exploratory-comparison
#17
Brice Guignard, Jessy Lauer, Pierre Samozino, Luis Mourão, João Paulo Vilas-Boas, Annie Hélène Rouard
During a horizontal underwater push-off, performance is strongly limited by the presence of water, inducing resistances due to its dense and viscous nature. At the same time, aquatic environments offer a support to the swimmer with the hydrostatic buoyancy counteracting the effects of gravity. Squat jump is a vertical terrestrial push-off with a maximal lower limb extension limited by the gravity force, which attracts the body to the ground. Following this observation, we characterized the effects of environment (water vs...
October 25, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/29084096/reliability-and-sensitivity-of-the-power-push-up-test-for-upper-body-strength-and-power-in-6-15-year-old-male-athletes
#18
Zachary M Gillen, Amelia A Miramonti, Brianna D McKay, Nathaniel D M Jenkins, Todd J Leutzinger, Joel T Cramer
The power push-up (PPU) test is an explosive upper-body test performed on a force plate and is currently being used in high school football combines throughout the U.S. The purpose of this study was to quantify the reliability of the PPU test based on age and starting position (knees vs. toes) in young athletes. Sixty-eight boys (mean ± standard deviation (SD); age = 10.8 ± 2.0 years) were tested twice over 5 days. Boys were separated by age as 6-9-years (n = 16), 10-11-years (n = 26), and 12-15-years (n = 26)...
October 27, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/29083127/influence-of-shoe-sole-bending-stiffness-on-sprinting-performance
#19
Ryu Nagahara, Hiroaki Kanehisa, Tetsuo Fukunaga
BACKGROUND: This study investigated the influence of shoe sole bending stiffness on sprint performance, in relation to anthropometric and strength-power capability characteristics of sprinters. METHODS: Seventeen male athletes performed three maximal effort 60-m sprints using spiked-shoes with different bending stiffness sole. Sprint times during 60-m sprint, anthropometry and strength-power capabilities represented by maximum toe-flexor test and rebound continuous ankle jump of athletes were measured...
October 27, 2017: Journal of Sports Medicine and Physical Fitness
https://www.readbyqxmd.com/read/29082488/guidelines-for-microplate-selection-in-high-content-imaging
#20
Oscar J Trask
Since the inception of commercialized automated high content screening (HCS) imaging devices in the mid to late 1990s, the adoption of media vessels typically used to house and contain biological specimens for interrogation has transitioned from microscope slides and petri dishes into multi-well microtiter plates called microplates. The early 96- and 384-well microplates commonly used in other high-throughput screening (HTS) technology applications were often not designed for optical imaging. Since then, modifications and the use of next-generation materials with improved optical clarity have enhanced the quality of captured images, reduced autofocusing failures, and empowered the use of higher power magnification objectives to resolve fine detailed measurements at the subcellular pixel level...
2018: Methods in Molecular Biology
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