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https://www.readbyqxmd.com/read/28105774/stiffness-controlled-thermoresponsive-hydrogels-for-cell-harvesting-with-sustained-mechanical-memory
#1
Xingliang Fan, Lu Zhu, Ke Wang, Bingjie Wang, Yaozu Wu, Wei Xie, Chengyu Huang, Barbara Pui Chan, Yanan Du
Most mechanobiological investigations focused on in situ mechanical regulation of cells on stiffness-controlled substrates with few downstream applications, as it is still challenging to harvest and expand mechanically primed cells by enzymatic digestion (e.g., trypsin) without interrupting cellular mechanical memory between passages. This study develops thermoresponsive hydrogels with controllable stiffness to generate mechanically primed cells with intact mechanical memory for augmented wound healing. No significant cellular property alteration of the fibroblasts primed on thermoresponsive hydrogels with varied stiffness has been observed through thermoresponsive harvesting...
January 20, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28105152/evaluation-by-quantitative-image-analysis-of-anticancer-drug-activity-on-multicellular-spheroids-grown-in-3d-matrices
#2
Aurélie Gomes, Adrien Russo, Guillaume Vidal, Elise Demange, Pauline Pannetier, Zied Souguir, Jean-Michel Lagarde, Bernard Ducommun, Valérie Lobjois
Pharmacological evaluation of anticancer drugs using 3D in vitro models provides invaluable information for predicting in vivo activity. Artificial matrices are currently available that scale up and increase the power of such 3D models. The aim of the present study was to propose an efficient and robust imaging and analysis pipeline to assess with quantitative parameters the efficacy of a particular cytotoxic drug. HCT116 colorectal adenocarcinoma tumor cell multispheres were grown in a 3D physiological hyaluronic acid matrix...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28102948/use-of-parallel-plate-ionization-chambers-in-reference-dosimetry-of-novac-and-liac-%C3%A2-mobile-electron-linear-accelerators-for-intraoperative-radiotherapy-a-multi-center-survey
#3
Paolo Scalchi, Alessia Ciccotelli, Giuseppe Felici, Assunta Petrucci, Raffaella Massafra, Valeria Piazzi, Paola D'Avenia, Francesca Cavagnetto, Federica Cattani, Raffaella Romagnoli, Antonella Soriani
PURPOSE: LIAC(®) and NOVAC are two mobile linear accelerators dedicated to intraoperative radiation therapy (IORT), generating electron beams in the energy range of 3-12 MeV. Due to high dose-per-pulse (up to 70 mGy per pulse), in 2003 the Italian National Institute of Health (ISS) stated that "for the measure of dose to water in reference conditions, ionization chambers cannot be employed and no published dosimetry protocol can be used". As a consequence, ferrous sulphate (or, alternatively alanine) dosimetry was recommended...
January 2017: Medical Physics
https://www.readbyqxmd.com/read/28102524/heat-transfer-capability-of-ethylene-glycol-water-based-nanofluids-containing-graphene-nanoplatelets-design-and-thermophysical-profile
#4
D Cabaleiro, L Colla, S Barison, L Lugo, L Fedele, S Bobbo
This research aims at studying the stability and thermophysical properties of nanofluids designed as dispersions of sulfonic acid-functionalized graphene nanoplatelets in an (ethylene glycol + water) mixture at (10:90)% mass ratio. Nanofluid preparation conditions were defined through a stability analysis based on zeta potential and dynamic light scattering (DLS) measurements. Thermal conductivity, dynamic viscosity, and density were experimentally measured in the temperature range from 283.15 to 343.15 K and nanoparticle mass concentrations of up to 0...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28098250/tailoring-femtosecond-1-5-%C3%AE-m-bessel-beams-for-manufacturing-high-aspect-ratio-through-silicon-vias
#5
Fei He, Junjie Yu, Yuanxin Tan, Wei Chu, Changhe Zhou, Ya Cheng, Koji Sugioka
Three-dimensional integrated circuits (3D ICs) are an attractive replacement for conventional 2D ICs as high-performance, low-power-consumption, and small-footprint microelectronic devices. However, one of the major remaining challenges is the manufacture of high-aspect-ratio through-silicon vias (TSVs), which is a crucial technology for the assembly of 3D Si ICs. Here, we present the fabrication of high-quality TSVs using a femtosecond (fs) 1.5-μm Bessel beam. To eliminate the severe ablation caused by the sidelobes of a conventional Bessel beam, a fs Bessel beam is tailored using a specially designed binary phase plate...
January 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28095386/in-vitro-evaluation-of-the-cleansing-effect-of-a-monofilament-fiber-debridement-pad-compared-to-gauze-swabs
#6
Cornelia Wiegand, Kirsten Reddersen, Uta-Christina Hipler, Martin Abel, Peter Ruth, Anneke Andriessen
BACKGROUND: Removal of nonvital tissue is an accepted method to eradicate biofilms and to stimulate wound healing. Debridement using a monofilament polyester fiber pad has clinically been shown to be effective as well as pain and trauma free. METHODS: For in vitro determination of the cleansing capacity of this product compared to gauze swabs, a wound debridement model was used with glass plates coated with a bovine serum albumin solution, stained with hematoxylin...
January 18, 2017: Skin Pharmacology and Physiology
https://www.readbyqxmd.com/read/28092630/comparative-monte-carlo-study-on-the-performance-of-integration-and-list-mode-detector-configurations-for-carbon-ion-computed-tomography
#7
Sebastian Meyer, Chiara Gianoli, Lorena Magallanes, Benedikt Kopp, Thomas Tessonnier, Guillaume Landry, George Dedes, Bernd Voss, Katia Parodi
Ion beam therapy offers the possibility of a highly conformal tumor-dose distribution; however, this technique is extremely sensitive to inaccuracies in the treatment procedures. Ambiguities in the conversion of Hounsfield units of the treatment planning x-ray CT to relative stopping power (RSP) can cause uncertainties in the estimated ion range of up to several millimeters. Ion CT (iCT) represents a favorable solution allowing to directly assess the RSP. In this simulation study we investigate the performance of the integration-mode configuration for carbon iCT, in comparison with a single-particle approach under the same set-up...
February 7, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28075392/a-distance-detector-with-a-strip-magnetic-mosfet-and-readout-circuit
#8
Guo-Ming Sung, Wen-Sheng Lin, Hsing-Kuang Wang
This paper presents a distance detector composed of two separated metal-oxide semiconductor field-effect transistors (MOSFETs), a differential polysilicon cross-shaped Hall plate (CSHP), and a readout circuit. The distance detector was fabricated using 0.18 μm 1P6M Complementary Metal-Oxide Semiconductor (CMOS) technology to sense the magnetic induction perpendicular to the chip surface. The differential polysilicon CSHP enabled the magnetic device to not only increase the magnetosensitivity but also eliminate the offset voltage generated because of device mismatch and Lorentz force...
January 10, 2017: Sensors
https://www.readbyqxmd.com/read/28072543/nanoscale-nucleation-and-growth-of-electrodeposited-lithium-metal
#9
Allen Pei, Guangyuan Zheng, Feifei Shi, Yuzhang Li, Yi Cui
Lithium metal has re-emerged as an exciting anode for high energy lithium-ion batteries due to its high specific capacity of 3,860 mAh g(-1) and lowest electrochemical potential of all known materials. However, lithium has been plagued by the issues of dendrite formation, high chemical reactivity with electrolyte, and infinite relative volume expansion during plating and stripping, which present safety hazards and low cycling efficiency in batteries with lithium metal electrodes. There have been a lot of recent studies on Li metal although little work has focused on the initial nucleation and growth behavior of Li metal, neglecting a critical fundamental scientific foundation of Li plating...
January 10, 2017: Nano Letters
https://www.readbyqxmd.com/read/28062003/identification-of-genes-involved-in-mycoplasma-gallisepticum-biofilm-formation-using-mini-tn4001-sgm-transposon-mutagenesis
#10
Yang Wang, Li Yi, Fanqing Zhang, Xusheng Qiu, Lei Tan, Shengqing Yu, Xiangchao Cheng, Chan Ding
Mycoplasma gallisepticum (MG) is an important pathogen that can cause chronic respiratory disease in chickens and infectious sinusitis in turkeys. MG has the ability to form biofilms. The molecular mechanisms underlying MG biofilm formation are complex and poorly understood. To better understand the mechanisms involved in biofilm formation, mini-Tn4001-SGM, a novel transposon vector containing the gentamicin gene was constructed and electroporated into MG strain Rlow. Of the 738 mutants obtained, 12 had significantly reduced capacity to form biofilms in a polystyrene microtiter-plate biofilm assay...
January 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28059236/characterization-of-coherence-or-power-selectable-operation-of-an-external-cavity-semiconductor-diode-laser
#11
Masaharu Hyodo, Masayoshi Watanabe, Akira Kawakami, Shingo Saito, Masaaki Adachi
The properties of the coherence-or-power selectable operation of an external-cavity semiconductor diode laser through the control of intracavity polarization states have been characterized in detail. In our technique, a diffraction grating and a reflector functioned as a polarization-dependent output coupler, such that the portion of light fed back to the gain medium was readily controlled by rotating the intracavity polarization axis, which resulted in the selectable operation of either a high degree of coherence or a high power for the laser output...
December 20, 2016: Applied Optics
https://www.readbyqxmd.com/read/28053900/the-possible-mode-of-antitussive-and-expectorant-activity-of-the-ethanol-seed-extracts-of-picralima-nitida-stapf-th-h-durand
#12
Gabriel Dapaah, George Asumeng Koffuor, Priscilla Kolibea Mante, Inemesit Okon Ben
It has been established that Picralima nitida has antitussive effect. This study therefore aimed at determining the possible mode of antitussive and expectorant activity of an ethanolic seed extract of P. nitida (PNE). The muco-suppressant, mast cell stabilization, and the anxiolytic effects of PNE were ascertained using ammonium chloride-induced phenol red secretion in BALB/c mice; compound 48/80-induced mesenteric mast cell degranulation assay; and the open field and the elevated plus maze models respectively...
January 2017: Journal of Traditional and Complementary Medicine
https://www.readbyqxmd.com/read/28048045/su-f-t-171-manufacturing-cost-effective-heterogeneous-phantoms-for-use-in-proton-therapy
#13
J Pruett, Y Chen, S Ahmad, D Johnson
PURPOSE: To study the feasibility of 3D printing cost effective heterogeneous phantoms for use in proton therapy treatment planning quality assurance. METHODS: A desktop 3D printer was utilized to create a series of 2 cm × 2 cm × 4 cm PLA plastic blocks of varying fill materials and hexagonal fill pattern. The blocks were than tested when filled with air, polyurethane foam, paraffin, silicone, and caulk of calcium carbonate - acrylic polymer blend. The blocks were evaluated with a "GE Lightspeed" 16 slice CT scanner and average CT# of the materials' centers evaluated...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28047296/su-f-t-213-commissioning-results-of-the-prototype-active-scanning-irradiation-system-of-korea-heavy-ion-medical-accelerator
#14
C Kim, J Seduk, T Yang
PURPOSE: A prototype actives scanning beam delivery system was designed, manufactured and installed as a part of the Korea Heavy Ion Medical Accelerator Project. The prototype system includes the most components for steering, modulating, detecting incident beam to patient. The system was installed in MC-50 cyclotron beam line and tested to extract the normal operation conditions. METHODS: The commissioning process was completed by using 45 MeV of proton beam. To measure the beam position accuracy along the scanning magnet power supply current, 25 different spots were scanning and measured...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28047047/su-d-brc-06-experimental-and-monte-carlo-studies-of-fluence-corrections-for-graphite-calorimetry-in-proton-therapy
#15
A Lourenco, R Thomas, H Bouchard, A Kacperek, V Vondracek, G Royle, H Palmans
PURPOSE: For photon and electron beams, the standard device used to measure absorbed dose is a calorimeter. Standards laboratories are currently working on the establishment of graphite calorimeters as a primary standard for proton beams. To provide a practical method for graphite calorimetry, it is necessary to convert dose to graphite to dose to water, requiring knowledge of the water-to-graphite stopping-power ratio and the fluence correction factor. This study aims to present a novel method to determine fluence corrections experimentally, and to apply this methodology to low- and high-energy proton beams...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28018068/customized-iliac-prosthesis-for-reconstruction-in-giant-cell-tumour-a-unique-treatment-approach
#16
Tarun Verma, Ankur Sharma, Amit Sharma, Lalit Maini
Giant cell tumour (GCT) of flat bones of pelvis is extremely rare. GCT of the ilium and ischium represent less than 0.05% of all GCT. Iliac bone GCT has been treated traditionally by intra-lesion curettage with bone grafting, wide resection with or without reconstruction and hemi-pelvectomy in very aggressive tumours. None of the above treatments were without morbidities. Reconstruction using bone grafts and bone cement has also been inadequate. In GCT, where life expectancy is not decreased significantly, surgical treatment should be aimed at giving optimum functional outcome...
October 2016: Journal of Clinical Orthopaedics and Trauma
https://www.readbyqxmd.com/read/28005850/temperature-insensitive-fiber-twist-sensor-based-on-elliptical-core-few-mode-fiber
#17
Dawei Yu, Qi Mo, Zhikun Hong, Songnian Fu, Chaotan Sima, Ming Tang, Deming Liu
A highly sensitive fiber optics twist sensor is proposed and experimentally demonstrated using elliptical-core few-mode fiber (e-FMF). With the help of a fixed-phase plate for conversion from LP<sub>11</sub> to LP<sub>01</sub> mode, the twist angle can be linearly mapped to the spatial rotation of LP<sub>11</sub> mode profile and consequently discriminated by monitoring the optical power variation at the standard single-mode fiber (SSMF) output. When the optical power at the e-FMF output is 10 mW, the twist angle within the range of ±39° can be successfully detected with a sensitivity of more than 20 μW/°...
October 15, 2016: Optics Letters
https://www.readbyqxmd.com/read/28002369/transient-local-bone-remodeling-effects-of-rhbmp-2-in-an-ovine-interbody-spine-fusion-model
#18
Hyun W Bae, Vikas V Patel, Zeeshan M Sardar, Jeffrey M Badura, Ben B Pradhan, Howard B Seim, A Simon Turner, Jeffrey M Toth
BACKGROUND: Recombinant human bone morphogenetic protein-2 (rhBMP-2) is a powerful osteoinductive morphogen capable of stimulating the migration of mesenchymal stem cells (MSCs) to the site of implantation and inducing the proliferation and differentiation of these MSCs into osteoblasts. Vertebral end-plate and vertebral body resorption has been reported after interbody fusion with high doses of rhBMP-2. In this study, we investigated the effects of 2 rhBMP-2 doses on peri-implant bone resorption and bone remodeling at 7 time points in an end-plate-sparing ovine interbody fusion model...
December 21, 2016: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/28000733/additive-and-photochemical-manufacturing-of-copper
#19
Winco K C Yung, Bo Sun, Zhengong Meng, Junfeng Huang, Yingdi Jin, Hang Shan Choy, Zhixiang Cai, Guijun Li, Cheuk Lam Ho, Jinlong Yang, Wai Yeung Wong
In recent years, 3D printing technologies have been extensively developed, enabling rapid prototyping from a conceptual design to an actual product. However, additive manufacturing of metals in the existing technologies is still cost-intensive and time-consuming. Herein a novel platform for low-cost additive manufacturing is introduced by simultaneously combining the laser-induced forward transfer (LIFT) method with photochemical reaction. Using acrylonitrile butadiene styrene (ABS) polymer as the sacrificial layer, sufficient ejection momentum can be generated in the LIFT method...
December 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27997838/ecology-and-fisheries-dark-carbon-on-your-dinner%C3%A2-plate
#20
Jillian M Petersen
Chemosynthetic primary production by symbiotic microbes powers entire ecosystems in the remote deep sea. New research shows that in shallow waters chemosynthetic symbioses can contribute substantially to a vital economic resource - lobster fisheries in the Caribbean Sea.
December 19, 2016: Current Biology: CB
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