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Christopher Ioannou, Matthew Knight, Luca Daniele, Lee Flueckiger, Ezekiel S L Tan
BACKGROUND: The objective of this study is to analyse the effectiveness of the surgical torque limiter during operative use. The study also investigates the potential differences in torque between hand and drill-based screw insertion into locking plates using a standardised torque limiter. METHODS: Torque for both hand and power screw insertion was measured through a load cell, registering 6.66 points per second. This was performed in a controlled environment using synthetic bone, a locking plate and locking screws to simulate plate fixation...
October 17, 2016: Journal of Orthopaedic Surgery and Research
Erika Zemková, Michal Jeleň, Peter Schickhofer, Dušan Hamar
: Background/Study Context: The study estimates the reliability of peak velocity and peak power during chair rising and chair jumping tests and their ability to discriminate between different age and physical activity level groups. METHODS: Physically active and sedentary individuals (N = 262) of different ages (young: 22.9 ± 2.0 years, range: 21-25 years; older: 63.1 ± 1.8 years, range: 61-65 years) performed, in random order, chair rising and chair jumping tests on a force plate...
October 2016: Experimental Aging Research
Hammad Ismail, Erum Dilshad, Mohammad Tahir Waheed, Bushra Mirza
Lettuce is an edible crop that is well known for dietary and antioxidant benefits. The present study was conducted to investigate the effects of rol ABC genes on antioxidant and medicinal potential of lettuce by Agrobacterium-mediated transformation. Transgene integration and expression was confirmed through PCR and real-time RT-PCR, respectively. The transformed plants showed 91-102 % increase in total phenolic contents and 53-65 % increase in total flavonoid contents compared to untransformed plants. Total antioxidant capacity and total reducing power increased up to 112 and 133 % in transformed plants, respectively...
October 12, 2016: Applied Biochemistry and Biotechnology
Georg Wandrey, Joel Wurzel, Kyra Hoffmann, Tobias Ladner, Jochen Büchs, Lorenz Meinel, Tessa Lühmann
BACKGROUND: Genetic code expansion has developed into an elegant tool to incorporate unnatural amino acids (uAA) at predefined sites in the protein backbone in response to an amber codon. However, recombinant production and yield of uAA comprising proteins are challenged due to the additional translation machinery required for uAA incorporation. RESULTS: We developed a microtiter plate-based high-throughput monitoring system (HTMS) to study and optimize uAA integration in the model protein enhanced green fluorescence protein (eGFP)...
2016: Journal of Biological Engineering
Imanuel Yüce, Gertrud E Morlock
A fast and reliable quality control is important for ink manufacturers to ensure a constant production grade of mixtures and chemical formulations, and unknown components attract their attention. Structure elucidating techniques seem time-consuming in combination with column-based methods, but especially the low solubility of pigment formulations is challenging the analysis. In contrast, layer chromatography is more tolerant with regard to pigment particles. One PLC plate for NMR and FTIR analyses and one HPTLC plate for recording of high resolution mass spectra, MS/MS spectra and for gathering information on polarity and spectral properties were needed to characterize a structure, exemplarily shown for an unknown component in pigment Red 57:1 to be 3-hydroxy-2-naphtoic acid...
October 21, 2016: Journal of Chromatography. A
Sin-Li Lee, Li-Ngee Ho, Soon-An Ong, Yee-Shian Wong, Chun-Hong Voon, Wan Fadhilah Khalik, Nik Athirah Yusoff, Noradiba Nordin
Photocatalytic fuel cell (PFC) is a potential wastewater treatment technology that can generate electricity from the conversion of chemical energy of organic pollutants. An immobilized ZnO/Zn fabricated by sonication and heat attachment method was applied as the photoanode and Pt/C plate was used as the cathode of the PFC in this study. Factors that affect the decolorization efficiency and electricity generation of the PFC such as different initial dye concentrations and pH were investigated. Results revealed that the degradation of Reactive Green 19 (RG19) was enhanced in a closed circuit PFC compared with that of a opened circuit PFC...
January 2017: Chemosphere
M Cihan Çakır, Deniz Çalışkan, Bayram Bütün, Ekmel Özbay
Metal oxide gas sensors with integrated micro-hotplate structures are widely used in the industry and they are still being investigated and developed. Metal oxide gas sensors have the advantage of being sensitive to a wide range of organic and inorganic volatile compounds, although they lack selectivity. To introduce selectivity, the operating temperature of a single sensor is swept, and the measurements are fed to a discriminating algorithm. The efficiency of those data processing methods strongly depends on temperature uniformity across the active area of the sensor...
2016: Sensors
Kristof Kipp, Philip J Malloy, Jordan Smith, Matthew D Giordanelli, Michael T Kiely, Christopher F Geiser, Timothy J Suchomel
The purpose of this study was to investigate the joint- and load-dependent changes in the mechanical demands of the lower extremity joints during the hang power clean (HPC) and the jump shrug (JS). Fifteen male lacrosse players were recruited from an NCAA DI team, and completed three sets of the HPC and JS at 30%, 50%, and 70% of their HPC 1-Repetition Maximum (1-RM HPC) in a counterbalanced and randomized order. Motion analysis and force plate technology were used to calculate the positive work, propulsive phase duration, and peak concentric power at the hip, knee, and ankle joints...
September 16, 2016: Journal of Strength and Conditioning Research
C C Avila, F G Almeida, G Palmisano
Accurate and rapid determination of trypanosomatids is essential in epidemiological surveillance and therapeutic studies. Matrix-assisted laser desorption ionization/time of flight mass spectrometry (MALDI-TOF MS) has been shown to be a useful and powerful technique to identify bacteria, fungi, metazoa and human intact cells with applications in clinical settings. Here, we developed and optimized a MALDI-TOF MS method to profile trypanosomatids. trypanosomatid cells were deposited on a MALDI target plate followed by addition of matrix solution...
August 2016: Journal of Mass Spectrometry: JMS
Youngmin Han, Kyoung-Tae Kim
OBJECTIVE: Recently, regenerative therapies have been used in clinical trials (heart, cartilage, skeletal). We don't make use of these treatments to spinal cord injury (SCI) patients yet, but regenerative therapies are rising interest in recent study about SCI. Neural precursor/stem cell (NPSC) proliferation is a significant event in functional recovery of the central nervous system (CNS). However, brain NPSCs and spinal cord NPSCs (SC-NPSCs) have many differences including gene expression and proliferation...
September 2016: Journal of Korean Neurosurgical Society
Ebrahim Abedi, Marzieh Ebrahimkhani, Amin Davari, Seyed Mohammad Mirvakili, Mohsen Tabasi, Mohammad Ghannadi Maragheh
Efficient and safe production of molybdenum-99 ((99)Mo) radiopharmaceutical at Tehran Research Reactor (TRR) via fission of LEU targets is studied. Neutronic calculations are performed to evaluate produced (99)Mo activity, core neutronic safety parameters and also the power deposition values in target plates during a 7 days irradiation interval. Thermal-hydraulic analysis has been also carried out to obtain thermal behavior of these plates. Using Thermal-hydraulic analysis, it can be concluded that the safety parameters are satisfied in the current study...
September 10, 2016: Applied Radiation and Isotopes
Kristie I Aamodt, Radhika Aramandla, Judy Brown, Nathalie Fiaschi-Taesch, Peng Wang, Andrew F Stewart, Marcela Brissova, Alvin C Powers
Numerous compounds stimulate rodent β cell proliferation; however, translating these findings to human β cells remains a challenge. To examine human β cell proliferation in response to such compounds, we developed a medium-throughput in vitro method of quantifying adult human β cell proliferation markers. This method is based on high-content imaging of dispersed islet cells seeded in 384-well plates and automated cell counting that identifies fluorescently-labeled β cells with high specificity using both nuclear and cytoplasmic markers...
September 13, 2016: American Journal of Physiology. Endocrinology and Metabolism
Mihaela Elena Marina, Carolina Solomon, Sorana-Daniela Bolboaca, Corina Bocsa, Carmen Mihaela Mihu, Alexandru Dumitru Tătaru
AIM: To evaluate the morphostructural aspects and nail vascularity in the nail unit of patients with psoriasis, and to evaluate whether there are differences among psoriatic patients with and without nail involvement. MATERIAL AND METHODS: Nail plates and nail bed changes, nail fold vessel resistance index (NVRI), power and color Doppler blood flow appearances were investigated in 23 patients with moderate-to-severe psoriasis, with and without nail involvement, and compared to those of 11 healthy participants...
September 2016: Medical Ultrasonography
Tahseen Chaudhry, Michelle Spiteri, Dominic Power, Mark Brewster
AIM: To review the results of our experience with the Medartis Aptus plating system for four corner arthrodesis of the wrist, which uses a combination of compression screws and variable angle locking screws. METHODS: We reviewed the results of 17 procedures in 16 patients that underwent scaphoid excision and four corner fusion using the Medartis Aptus system between May 2010 and June 2014. The primary outcome measure was radiographic and clinical union. RESULTS: The mean clinical follow up time was 20...
August 18, 2016: World Journal of Orthopedics
Betty Freret-Hodara, Yi Cui, Amélie Griveau, Lisa Vigier, Yoko Arai, Jonathan Touboul, Alessandra Pierani
Loss of neurons in the neocortex is generally thought to result in a final reduction of cerebral volume. Yet, little is known on how the developing cerebral cortex copes with death of early-born neurons. Here, we tackled this issue by taking advantage of a transgenic mouse model in which, from early embryonic stages to mid-corticogenesis, abundant apoptosis is induced in the postmitotic compartment. Unexpectedly, the thickness of the mutant cortical plate at E18.5 was normal, due to an overproduction of upper layer neurons at E14...
September 12, 2016: Cerebral Cortex
Tanja Stahl, Caroline Rothe, Manja U Böhme, Aloisa Kohl, Nicolaus Kröger, Boris Fehse
Accurate and sensitive determination of hematopoietic chimerism is a crucial diagnostic measure after allogeneic stem cell transplantation to monitor engraftment and potentially residual disease. Short tandem repeat (STR) amplification, the current "gold standard" for chimerism assessment facilitates reliable accuracy, but is hampered by its limited sensitivity (≥1%). Digital PCR (dPCR) has been shown to combine exact quantification and high reproducibility over a very wide measurement range with excellent sensitivity (routinely ≤0...
2016: International Journal of Molecular Sciences
Jiong Zeng, Yanhui Cui, Deyang Qu, Qian Zhang, Junwei Wu, Xiaomeng Zhu, Zuohua Li, Xinhe Zhang
Lithium-rich layered oxides are promising cathode candidates for the production of high-energy and high-power electronic devices with high specific capacity and high discharge voltage. However, unstable cycling performance, especially at high charge-recharge rate, is the most challenge issue which needs to be solved to foster the diffusion of these materials. In this paper, hierarchical platelike Li1.2Mn0.54Ni0.13Co0.13O2 cathode materials were synthesized by a facile solvothermal method followed by calcination...
October 5, 2016: ACS Applied Materials & Interfaces
Shirin Fahimi, Seyed Alireza Mortazavi, Mohammad Abdollahi, Homa Hajimehdipoor
Nowadays, plants have been considered as powerful agents for treatment of disorders regarding to their traditional use. In Iranian Traditional Medicine (ITM), plants have a special role in the treatment of various diseases. Burns with their devastating outcomes have been discussed in ITM as well. In the present study, a polyherbal ointment (PHO), retrieved from ITM, was formulated for burn healing and it's HPTLC fingerprint was prepared. Aqueous extracts of Malva sylvestris and Solanum nigrum leaves and oily extract of Rosa damascena petals (4...
2016: Iranian Journal of Pharmaceutical Research: IJPR
Yinggang Li, Ling Zhu, Tianning Chen
In this paper, we numerically and experimentally demonstrate the low-frequency broadband elastic wave attenuation and vibration suppression by using plate-type elastic metamaterial, which is constituted of periodic double-sides stepped resonators deposited on a two-dimensional phononic plate with steel matrix. The dispersion relations, the power transmission spectra, and the displacement fields of the eigenmodes are calculated by using the finite element method. In contrast to the typical phononic plates consisting of periodic stepped resonators deposited on a homogeneous steel plate, the proposed elastic metamaterial can yield large band gap in the low-frequency range, resulting in the low-frequency broadband elastic wave attenuation...
August 26, 2016: Ultrasonics
Mun-Gwan Hong, Woojoo Lee, Peter Nilsson, Yudi Pawitan, Jochen M Schwenk
Enhanced by the growing number of biobanks, biomarker studies can now be performed with reasonable statistical power by using large sets of samples. Antibody-based proteomics by means of suspension bead arrays offers one attractive approach to analyze serum, plasma, or CSF samples for such studies in microtiter plates. To expand measurements beyond single batches, with either 96 or 384 samples per plate, suitable normalization methods are required to minimize the variation between plates. Here we propose two normalization approaches utilizing MA coordinates...
October 7, 2016: Journal of Proteome Research
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