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MicroRNA and pain

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https://www.readbyqxmd.com/read/27922111/mir-365-targets-%C3%AE-arrestin-2-to-reverse-morphine-tolerance-in-rats
#1
Jian Wang, Wei Xu, Tao Zhong, Zongbin Song, Yu Zou, Zhuofeng Ding, Qulian Guo, Xinzhong Dong, Wangyuan Zou
Morphine tolerance is a challenging clinical problem that limits its clinical application in pain treatment. Non-coding microRNAs (miRNAs) modulate gene expression in a post transcriptional manner, and their dysregulation causes various diseases. However, the significance of miRNAs in morphine tolerance is still poorly understood. In the present study, we hypothesized that microRNA-365 (miR-365) is a key functional small RNA that reverses morphine tolerance through regulation of β-arrestin 2 in rats. Here, microarray analysis and quantitative real-time PCR showed that miR-365 was robustly decreased in the spinal cord after chronic morphine administration...
December 6, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27920341/serum-mir-451a-levels-are-significantly-elevated-in-women-with-endometriosis-and-recapitulated-in-baboons-papio-anubis-with-experimentally-induced-disease
#2
Warren B Nothnick, Tommaso Falcone, Niraj Joshi, Asgerally T Fazleabas, Amanda Graham
We have previously demonstrated that human microRNA-451a (miR-451a) endometriotic lesion expression is significantly higher compared to that of the corresponding eutopic endometrium. The objective of the current study was to examine the relationship between lesion and serum content of miR-451a and to determine the utility of serum miR-451a in distinguishing between women with and without visible signs of endometriosis. Eighty-one participants were enrolled in this study, 41 with confirmed endometriosis and 40 without visible signs of endometriosis at laparoscopy (n = 20) or symptoms of endometriosis (pain, infertility n = 20)...
December 5, 2016: Reproductive Sciences
https://www.readbyqxmd.com/read/27917453/phase-i-study-of-mrx34-a-liposomal-mir-34a-mimic-administered-twice-weekly-in-patients-with-advanced-solid-tumors
#3
Muhammad S Beg, Andrew J Brenner, Jasgit Sachdev, Mitesh Borad, Yoon-Koo Kang, Jay Stoudemire, Susan Smith, Andreas G Bader, Sinil Kim, David S Hong
Purpose Naturally occurring tumor suppressor microRNA-34a (miR-34a) downregulates the expression of >30 oncogenes across multiple oncogenic pathways, as well as genes involved in tumor immune evasion, but is lost or under-expressed in many malignancies. This first-in-human, phase I study assessed the maximum tolerated dose (MTD), safety, pharmacokinetics, and clinical activity of MRX34, a liposomal miR-34a mimic, in patients with advanced solid tumors. Patients and Methods Adult patients with solid tumors refractory to standard treatment were enrolled in a standard 3 + 3 dose escalation trial...
December 5, 2016: Investigational New Drugs
https://www.readbyqxmd.com/read/27893432/microrna-34a-expression-levels-in-serum-and-intratumoral-tissue-can-predict-bone-metastasis-in-patients-with-hepatocellular-carcinoma
#4
Zuo-Lin Xiang, Xiao-Mei Zhao, Li Zhang, Ping Yang, Jia Fan, Zhao-You Tang, Zhao-Chong Zeng
Hepatocellular carcinoma (HCC) patients with bone metastasis (BM) suffer from pain and other symptoms that significantly reduce their quality of life. We screened a microRNA (miRNA) microarray to identify potential serum biomarkers for BM in HCC patients. A miRNA microarray was used to screen for BM-related miRNAs in paired serum samples from HCC patients with BM and from HCC patients without BM. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to quantify candidate miRNAs in serum samples from 106 independent HCC patients...
November 23, 2016: Oncotarget
https://www.readbyqxmd.com/read/27805929/genetic-variant-rs3750625-in-the-3-utr-of-adra2a-affects-stress-dependent-acute-pain-severity-after-trauma-and-alters-a-microrna-34a-regulatory-site
#5
Sarah D Linnstaedt, Margaret G Walker, Kyle D Riker, Jennifer E Nyland, JunMei Hu, Catherine Rossi, Robert A Swor, Jeffrey S Jones, Luda Diatchenko, Andrey V Bortsov, David A Peak, Samuel A McLean
α2A adrenergic receptor (α2A-AR) activation has been shown in animal models to play an important role in regulating the balance of acute pain inhibition vs. facilitation after both physical and psychological stress. To our knowledge the influence of genetic variants in the gene encoding α2A-AR, ADRA2A, on acute pain outcomes in humans experiencing traumatic stress has not been assessed. In this study, we tested whether a genetic variant in the 3'UTR of ADRA2A, rs3750625, is associated with acute musculoskeletal pain (MSP) severity following motor vehicle collision (MVC, n = 948) and sexual assault (n = 84), and whether this influence was affected by stress severity...
October 25, 2016: Pain
https://www.readbyqxmd.com/read/27770129/microrna-16-alleviates-inflammatory-pain-by-targeting-ras-related-protein-23-rab23-and-inhibiting-p38-mapk-activation
#6
Wenjin Chen, Shengdong Guo, Shenggang Wang
BACKGROUND The purpose of our study was to determine the functional role of microRNA (miR)-16 in chronic inflammatory pain and to disclose its underlying molecular mechanism. MATERIAL AND METHODS Inflammatory pain was induced by injection of complete Freund's adjuvant (CFA) to Wistar rats. The pWPXL-miR-16, PcDNA3.1- Ras-related protein (RAB23), and/or SB203580 were delivered intrathecally to the rats. Behavioral tests were detected at 0 h, 4 h, 1 d, 4 d, 7 d, and 14 d after CFA injection. After behavioral tests, L4-L6 dorsal spinal cord were obtained and the levels of miR-16, RAB23, and phosphorylation of p38 (p-p38) were evaluated by quantitative real-time PCR (qRT-PCR)...
October 22, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27765894/microrna-30b-regulates-expression-of-the-sodium-channel-nav1-7-in-nerve-injury-induced-neuropathic-pain-in-the-rat
#7
Jinping Shao, Jing Cao, Jiannan Wang, Xiuhua Ren, Songxue Su, Ming Li, Zhihua Li, Qingzan Zhao, Weidong Zang
Voltage-gated sodium channels, which are involved in pain pathways, have emerged as major targets for therapeutic intervention in pain disorders. Nav1.7, the tetrodotoxin-sensitive voltage-gated sodium channel isoform encoded by SCN9A and predominantly expressed in pain-sensing neurons in the dorsal root ganglion, plays a crucial role in nociception. MicroRNAs are highly conserved, small non-coding RNAs. Through binding to the 3' untranslated region of their target mRNAs, microRNAs induce the cleavage and/or inhibition of protein translation...
2016: Molecular Pain
https://www.readbyqxmd.com/read/27699225/identification-of-microrna-181a-5p-and-microrna-4454-as-mediators-of-facet-cartilage-degeneration
#8
Akihiro Nakamura, Y Raja Rampersaud, Anirudh Sharma, Stephen J Lewis, Brian Wu, Poulami Datta, Kala Sundararajan, Helal Endisha, Evgeny Rossomacha, Jason S Rockel, Igor Jurisica, Mohit Kapoor
Osteoarthritis (OA) of spine (facet joints [FJs]) is one of the major causes of severe low back pain and disability worldwide. The degeneration of facet cartilage is a hallmark of FJ OA. However, endogenous mechanisms that initiate degeneration of facet cartilage are unknown, and there are no disease-modifying therapies to stop FJ OA. In this study, we have identified microRNAs (small noncoding RNAs) as mediators of FJ cartilage degeneration. We first established a cohort of patients with varying degrees of facet cartilage degeneration (control group: normal or mild facet cartilage degeneration; FJ OA group: moderate to severe facet cartilage degeneration) and then screened 2,100 miRNAs and identified 2 miRNAs (miR-181a-5p and miR-4454) that were significantly elevated in FJ OA cartilage compared with control facet cartilage...
August 4, 2016: JCI Insight
https://www.readbyqxmd.com/read/27697141/follicular-t-helper-cells-and-il-21-in-rheumatic-diseases
#9
Tue Kruse Rasmussen
Rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) are lifelong diseases with increased mortality and chronic pains. They are both characterized by immunological imbalances causing the immune system attack and destroy the bodies own tissues (called autoimmune disease). The best treatment, we are currently able to offer these patients, cause significant side-effects and can not prevent significant loss of quality of life. At the heart of the disease mechanisms in RA and SLE are subsets of immune cells called T and B cells...
October 2016: Danish Medical Journal
https://www.readbyqxmd.com/read/27690835/microrna-320a-predicts-chronic-axial-and-widespread-pain-development-following-motor-vehicle-collision-in-a-stress-dependent-manner
#10
Sarah D Linnstaedt, Kyle D Riker, Margaret G Walker, Jennifer E Nyland, Erin Zimny, Christopher Lewandowski, Phyllis L Hendry, Kathia Damiron, Claire Pearson, Marc-Anthony Velilla, Jeffrey Jones, Robert A Swor, Robert Domeier, Samuel A McLean
Study Design Prospective human cohort study combined with molecular studies. Background A microRNA is a small, noncoding RNA molecule that can play a role in disease onset. Recent studies found that circulating levels of microRNA 320a (miR-320a) are associated with musculoskeletal pain conditions and that miR-320a is stress responsive. Objectives To investigate whether circulating expression levels of miR-320a in the peritraumatic period predict persistent axial musculoskeletal pain 6 months after motor vehicle collision (MVC)...
October 2016: Journal of Orthopaedic and Sports Physical Therapy
https://www.readbyqxmd.com/read/27646435/mir-124a-and-mir-155-enhance-differentiation-of-regulatory-t-cells-in-patients-with-neuropathic-pain
#11
Jens Heyn, Benjamin Luchting, Ludwig C Hinske, Max Hübner, Shahnaz C Azad, Simone Kreth
BACKGROUND: Accumulating evidence indicates that neuropathic pain is a neuro-immune disorder with enhanced activation of the immune system. Recent data provided proof that neuropathic pain patients exhibit increased numbers of immunosuppressive regulatory T cells (Tregs), which may represent an endogenous attempt to limit inflammation and to reduce pain levels. We here investigate the molecular mechanisms underlying these alterations. METHODS: Our experimental approach includes functional analyses of primary human T cells, 3'-UTR reporter assays, and expression analyses of neuropathic pain patients' samples...
2016: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/27612948/secreted-trophic-factors-of-mesenchymal-stem-cells-support-neurovascular-and-musculoskeletal-therapies
#12
REVIEW
Heidi R Hofer, Rocky S Tuan
Adult mesenchymal stem cells (MSCs) represent a subject of intense experimental and biomedical interest. Recently, trophic activities of MSCs have become the topic of a number of revealing studies that span both basic and clinical fields. In this review, we focus on recent investigations that have elucidated trophic mechanisms and shed light on MSC clinical efficacy relevant to musculoskeletal applications. Innate differences due to MSC sourcing may play a role in the clinical utility of isolated MSCs. Pain management, osteochondral, nerve, or blood vessel support by MSCs derived from both autologous and allogeneic sources have been examined...
September 9, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27599867/downregulation-of-mir-219-enhances-brain-derived-neurotrophic-factor-production-in-mouse-dorsal-root-ganglia-to-mediate-morphine-analgesic-tolerance-by-upregulating-camkii%C3%AE
#13
Xue-Ming Hu, Shou-Bin Cao, Hai-Long Zhang, Dong-Mei Lyu, Li-Ping Chen, Heng Xu, Zhi-Qiang Pan, Wen Shen
BACKGROUND: Increasing evidence suggests that microRNAs are functionally involved in the initiation and maintenance of pain hypersensitivity, including chronic morphine analgesic tolerance, through the posttranscriptional regulation of pain-related genes. We have previously demonstrated that miR-219 regulates inflammatory pain in the spinal cord by targeting calcium/calmodulin-dependent protein kinase II gamma (CaMKIIγ). However, whether miR-219 regulates CaMKIIγ expression in the dorsal root ganglia to mediate morphine tolerance remains unclear...
2016: Molecular Pain
https://www.readbyqxmd.com/read/27515563/epigallocatechin-3-o-gallate-up-regulates-microrna-199a-3p-expression-by-down-regulating-the-expression-of-cyclooxygenase-2-in-stimulated-human-osteoarthritis-chondrocytes
#14
Zafar Rasheed, Naila Rasheed, Hani A Al-Shobaili
Osteoarthritis (OA) is a most common form of arthritis worldwide leading to significant disability. MicroRNAs (miRNAs) are non-coding RNAs involved in various aspects of cartilage development, homoeostasis and pathology. Several miRNAs have been identified which have shown to regulate expression of target genes relevant to OA pathogenesis such as matrix metalloproteinase (MMP)-13, cyclooxygenase (COX)-2, etc. Epigallocatechin-3-O-gallate (EGCG), the most abundant and active polyphenol in green tea, has been reported to have anti-arthritic effects, however, the role of EGCG in the regulation of miRNAs has not been investigated in OA...
August 12, 2016: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/27498764/regulation-of-proinflammatory-genes-by-the-circulating-microrna-hsa-mir-939
#15
Marguerite K McDonald, Sujay Ramanathan, Andrew Touati, Yiqian Zhou, Rushi U Thanawala, Guillermo M Alexander, Ahmet Sacan, Seena K Ajit
Circulating microRNAs are beneficial biomarkers because of their stability and dysregulation in diseases. Here we sought to determine the role of miR-939, a miRNA downregulated in patients with complex regional pain syndrome (CRPS). Hsa-miR-939 is predicted to target several proinflammatory genes, including IL-6, VEGFA, TNFα, NFκB2, and nitric oxide synthase 2 (NOS2A). Binding of miR-939 to the 3' untranslated region of these genes was confirmed by reporter assay. Overexpression of miR-939 in vitro resulted in reduction of IL-6, NOS2A and NFκB2 mRNAs, IL-6, VEGFA, and NOS2 proteins and NFκB activation...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27446561/cancer-of-unknown-primary-ultimately-diagnosed-as-male-breast-cancer-a-rare-case-report
#16
Georgios Rigakos, Amanda Vakos, Sotirios Papadopoulos, Anastasia Vernadou, Antonios Tsimpidakis, Dionysios Papachristou, Evangelia Razis
Cancers of unknown primary (CUP) constitute a significant diagnostic and therapeutic challenge for clinicians and a frequent cause of cancer-related mortality in Western countries. Immunohistochemistry assays are commonly used to identify the primary cancer, but fail in approximately one-third of cases. The identification of the possible origin of CUP is crucial, as it may help select the appropriate treatment options. We herein present the case of a 54-year-old male patient, who presented with lower back pain in June, 2013...
August 2016: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/27438293/on-the-prospect-of-clinical-utilization-of-micrornas-as-biomarkers-or-treatment-of-chronic-pain-a-comment-on-increased-mir-132-3p-expression-is-associated-with-chronic-neuropathic-pain
#17
Hjalte H Andersen, Kasper B Johnsen, Lars Arendt-Nielsen
No abstract text is available yet for this article.
July 17, 2016: Experimental Neurology
https://www.readbyqxmd.com/read/27438143/microrna-93-5p-may-participate-in-the-formation-of-morphine-tolerance-in-bone-cancer-pain-mouse-model-by-targeting-smad5
#18
Wen-Feng Xiao, Yu-Sheng Li, Wei Lou, Ting Cai, Shun Zhang, Xiao-Ying Hu, Xing-Wang Zhang, Wei Luo
OBJECTIVE: In this study, we aim to find out the role of microRNA-93-5p (miR-93) and Smad5 in morphine tolerance in mouse models of bone cancer pain (BCP). RESULTS: At 7 days after injection of morphine, the PMWT showed no significant difference between the morphine model group and the saline model group (P < 0.05), suggesting that morphine tolerance had formed in the morphine model group. The morphine model group had higher miR-93 expression and lower Smad5 mRNA expression than the saline model group...
July 11, 2016: Oncotarget
https://www.readbyqxmd.com/read/27429177/increased-cutaneous-mir-let-7d-expression-correlates-with-small-nerve-fiber-pathology-in-patients-with-fibromyalgia-syndrome
#19
Mathias Leinders, Kathrin Doppler, Thomas Klein, Maximiliane Deckart, Heike Rittner, Claudia Sommer, Nurcan Üçeyler
Fibromyalgia syndrome (FMS) is a chronic widespread pain condition probably comprising subgroups with different underlying pathomechanisms. There is increasing evidence for small nerve fiber impairment in subgroups of patients with FMS. MicroRNAs (miRNA) regulate molecular factors determining nerve de- and regeneration. We investigated whether systemic and cutaneous miRNA expression in FMS patients is related to small nerve fiber pathology. We confirmed previous findings of disturbed small fiber function and of reduced intraepidermal nerve fiber density in subgroups of patients with FMS...
July 15, 2016: Pain
https://www.readbyqxmd.com/read/27416044/emerging-biomarkers-for-the-diagnosis-and-monitoring-of-inflammatory-bowel-diseases
#20
Anet A Soubières, Andrew Poullis
There is currently no single test available to confidently diagnose cases of inflammatory bowel disease (IBD). Physicians rely on a number of diagnostic tools, including clinical evaluation, serum testing, and imaging, which are used on conjunction with endoscopic evaluation. It is often difficult to determine whether patients with abdominal pain and change in bowel habit have functional bowel symptoms or whether they have a true diagnosis of IBD. Even once a diagnosis of IBD has been made, a significant proportion of patients are labeled with the term "indeterminate colitis" where histological sampling cannot confidently subclassify patients as either Crohn's or ulcerative colitis...
August 2016: Inflammatory Bowel Diseases
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