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mechanosensitive channel

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https://www.readbyqxmd.com/read/29118717/identification-of-intracellular-%C3%AE-barrel-residues-involved-in-ion-selectivity-in-the-mechanosensitive-channel-of-thermoanaerobacter-tengcongensis
#1
Yingcai Song, Bing Zhang, Fei Guo, Maojun Yang, Yang Li, Zhi-Qiang Liu
The mechanosensitive channel of small conductance (MscS) is a bacterial membrane pore that senses membrane tension and protects cells from lysis by releasing osmolytes. MscS is a homoheptameric channel with a cytoplasmic domain with seven portals and a β-barrel opening to the cytoplasm. TtMscS, an MscS channel from Thermoanaerobacter tengcongensis, is an anion-selective channel. A previous study from our laboratory has defined the crucial role of β-barrel in the anion selectivity of TtMscS (Zhang et al., 2012)...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29117521/sparse-labeling-peldor-spectroscopy-on-multimeric-mechanosensitive-membrane-channels
#2
Katrin Ackermann, Christos Pliotas, Silvia Valera, James H Naismith, Bela E Bode
Pulse electron paramagnetic resonance (EPR) is being applied to ever more complex biological systems comprising multiple subunits. Membrane channel proteins are of great interest as pulse EPR reports on functionally significant but distinct conformational states in a native environment without the need for crystallization. Pulse EPR, in the form of pulsed electron-electron double resonance (PELDOR), using site-directed spin labeling, is most commonly employed to accurately determine distances (in the nanometer range) between different regions of the structure...
November 7, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/29074777/second-messenger-mediated-tactile-response-by-a-bacterial-rotary-motor
#3
Isabelle Hug, Siddharth Deshpande, Kathrin S Sprecher, Thomas Pfohl, Urs Jenal
When bacteria encounter surfaces, they respond with surface colonization and virulence induction. The mechanisms of bacterial mechanosensation and downstream signaling remain poorly understood. Here, we describe a tactile sensing cascade in Caulobacter crescentus in which the flagellar motor acts as sensor. Surface-induced motor interference stimulated the production of the second messenger cyclic diguanylate by the motor-associated diguanylate cyclase DgcB. This led to the allosteric activation of the glycosyltransferase HfsJ to promote rapid synthesis of a polysaccharide adhesin and surface anchoring...
October 27, 2017: Science
https://www.readbyqxmd.com/read/29058189/studying-mechanosensitivity-of-two-pore-domain-k-channels-in-cellular-and-reconstituted-proteoliposome-membranes
#4
Josefina Del Mármol, Robert A Rietmeijer, Stephen G Brohawn
Mechanical force sensation is fundamental to a wide breadth of biology from the classic senses of touch, pain, hearing, and balance to less conspicuous sensations of proprioception, blood pressure, and osmolarity and basic aspects of cell growth, differentiation, and development. These diverse and essential systems use force-gated (or mechanosensitive) ion channels that convert mechanical stimuli into cellular electrical signals. TRAAK, TREK1, and TREK2 are K(+)-selective ion channels of the two-pore domain K(+) (K2P) family that are mechanosensitive: they are gated open by increasing membrane tension...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29044106/a-mechanosensitive-ca-2-channel-activity-is-dependent-on-the-developmental-regulator-dek1
#5
Daniel Tran, Roberta Galletti, Enrique D Neumann, Annick Dubois, Reza Sharif-Naeini, Anja Geitmann, Jean-Marie Frachisse, Olivier Hamant, Gwyneth C Ingram
Responses of cells to mechanical stress are thought to be critical in coordinating growth and development. Consistent with this idea, mechanically activated channels play important roles in animal development. For example, the PIEZO1 channel controls cell division and epithelial-layer integrity and is necessary for vascular development in mammals. In plants, the actual contribution of mechanoperception to development remains questionable because very few putative mechanosensors have been identified and the phenotypes of the corresponding mutants are rather mild...
October 18, 2017: Nature Communications
https://www.readbyqxmd.com/read/28986054/reduced-aggrecan-expression-affects-cardiac-outflow-tract-development-in-zebrafish-and-is-associated-with-bicuspid-aortic-valve-disease-in-humans
#6
Pierre Rambeau, Emilie Faure, Alexis Théron, Jean-François Avierinos, Chris Jopling, Stéphane Zaffran, Adèle Faucherre
Hemodynamic forces have been known for a long time to regulate cardiogenic processes such as cardiac valve development. During embryonic development in vertebrates, the outflow tract (OFT) adjacent to the ventricle comes under increasing hemodynamic load as cardiogenesis proceeds. Consequently, extracellular matrix components are produced in this region as the cardiac cushions form which will eventually give rise to the aortic valves. The proteoglycan AGGRECAN is a key component of the aortic valves and is frequently found to be deregulated in a variety of aortic valve diseases...
December 15, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28983916/piezo2-as-the-anomalous-mechanotransducer-channel-in-auditory-hair-cells
#7
Maryline Beurg, Robert Fettiplace
Throughout post-natal maturation of the mouse inner ear, cochlear hair cells display at least two types of mechanically-gated ion channel: normal mechanotransducer (MT) channels at the tips of the stereocilia, activated by tension in inter-ciliary tip links; and anomalous mechanosensitive (MS) channels on the top surface of the cells. The anomalous MS channels are responsible for the reverse-polarity current that appears in mutants in which normal transduction is lost. They are also seen in wild-type hair cells around birth, appearing two days earlier than normal MT channels, and being down-regulated with the emergence of the normal channels...
October 5, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28952491/biophysical-tools-to-study-cellular-mechanotransduction
#8
REVIEW
Ismaeel Muhamed, Farhan Chowdhury, Venkat Maruthamuthu
The cell membrane is the interface that volumetrically isolates cellular components from the cell's environment. Proteins embedded within and on the membrane have varied biological functions: reception of external biochemical signals, as membrane channels, amplification and regulation of chemical signals through secondary messenger molecules, controlled exocytosis, endocytosis, phagocytosis, organized recruitment and sequestration of cytosolic complex proteins, cell division processes, organization of the cytoskeleton and more...
February 7, 2017: Bioengineering
https://www.readbyqxmd.com/read/28950473/cooperative-response-and-clustering-consequences-of-membrane-mediated-interactions-among-mechanosensitive-channels
#9
Lucas D Fernandes, Ksenia Guseva, Alessandro P S de Moura
Mechanosensitive channels are ion channels which act as cells' safety valves, opening when the osmotic pressure becomes too high and making cells avoid damage by releasing ions. They are found on the cellular membrane of a large number of organisms. They interact with each other by means of deformations they induce in the membrane. We show that collective dynamics arising from the interchannel interactions lead to first- and second-order phase transitions in the fraction of open channels in equilibrium relating to the formation of channel clusters...
August 2017: Physical Review. E
https://www.readbyqxmd.com/read/28947806/premature-contractions-of-the-bladder-are-suppressed-by-interactions-between-trpv4-and-sk3-channels-in-murine-detrusor-pdgfr%C3%AE-cells
#10
Haeyeong Lee, Byoung H Koh, Lauren E Peri, Robert D Corrigan, Hyun-Tai Lee, Nikita E George, Bhupal P Bhetwal, Yeming Xie, Brian A Perrino, Toby C Chai, Kenton M Sanders, Sang Don Koh
During filling, urinary bladder volume increases dramatically with little change in pressure. This is accomplished by suppressing contractions of the detrusor muscle that lines the bladder wall. Mechanisms responsible for regulating detrusor contraction during filling are poorly understood. Here we describe a novel pathway to stabilize detrusor excitability involving platelet-derived growth factor receptor-α positive (PDGFRα(+)) interstitial cells. PDGFRα(+) cells express small conductance Ca(2+)-activated K(+) (SK) and TRPV4 channels...
September 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28923939/asymmetric-mechanosensitivity-in-a-eukaryotic-ion-channel
#11
Michael V Clausen, Viwan Jarerattanachat, Elisabeth P Carpenter, Mark S P Sansom, Stephen J Tucker
Living organisms perceive and respond to a diverse range of mechanical stimuli. A variety of mechanosensitive ion channels have evolved to facilitate these responses, but the molecular mechanisms underlying their exquisite sensitivity to different forces within the membrane remains unclear. TREK-2 is a mammalian two-pore domain (K2P) K(+) channel important for mechanosensation, and recent studies have shown how increased membrane tension favors a more expanded conformation of the channel within the membrane...
October 3, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28921397/a-binding-block-ion-selective-mechanism-revealed-by-a-na-k-selective-channel
#12
Jie Yu, Bing Zhang, Yixiao Zhang, Cong-Qiao Xu, Wei Zhuo, Jingpeng Ge, Jun Li, Ning Gao, Yang Li, Maojun Yang
Mechanosensitive (MS) channels are extensively studied membrane protein for maintaining intracellular homeostasis through translocating solutes and ions across the membrane, but its mechanisms of channel gating and ion selectivity are largely unknown. Here, we identified the YnaI channel as the Na(+)/K(+) cation-selective MS channel and solved its structure at 3.8 Å by cryo-EM single-particle method. YnaI exhibits low conductance among the family of MS channels in E. coli, and shares a similar overall heptamer structure fold with previously studied MscS channels...
September 18, 2017: Protein & Cell
https://www.readbyqxmd.com/read/28905996/merkel-cells-and-meissner-s-corpuscles-in-human-digital-skin-display-piezo2-immunoreactivity
#13
Y García-Mesa, J García-Piqueras, B García, J Feito, R Cabo, J Cobo, J A Vega, O García-Suárez
The transformation of mechanical energy into electrical signals is the first step in mechanotransduction in the peripheral sensory nervous system and relies on the presence of mechanically gated ion channels within specialized sensory organs called mechanoreceptors. Piezo2 is a vertebrate stretch-gated ion channel necessary for mechanosensitive channels in mammalian cells. Functionally, it is related to light touch, which has been detected in murine cutaneous Merkel cell-neurite complexes, Meissner-like corpuscles and lanceolate nerve endings...
September 14, 2017: Journal of Anatomy
https://www.readbyqxmd.com/read/28905417/investigating-the-structural-dynamics-of-the-piezo1-channel-activation-and-inactivation-by-coarse-grained-modeling
#14
Wenjun Zheng, Frederick Sachs
The PIEZO channels, a family of mechanosensitive channels in vertebrates, feature a fast activation by mechanical stimuli (eg, membrane tension) followed by a slower inactivation. Although a medium-resolution structure of the trimeric form of PIEZO1 was solved by cryo-electron microscopy (cryo-EM), key structural changes responsible for the channel activation and inactivation are still unknown. Toward decrypting the structural mechanism of the PIEZO1 activation and inactivation, we performed systematic coarse-grained modeling using an elastic network model and related modeling/analysis tools (ie, normal mode analysis, flexibility and hotspot analysis, correlation analysis, and cryo-EM-based hybrid modeling and flexible fitting)...
September 14, 2017: Proteins
https://www.readbyqxmd.com/read/28882752/mechanosensitive-ion-channels-in-articular-nociceptors-drive%C3%A2-mechanical-allodynia-in-osteoarthritis
#15
B H He, M Christin, S Mouchbahani-Constance, A Davidova, R Sharif-Naeini
OBJECTIVE: Osteoarthritis (OA) is a disabling and highly prevalent condition affecting millions worldwide. Pain is the major complaint of OA patients and is presently inadequately managed. It manifests as mechanical allodynia, a painful response to innocuous stimuli such as joint movement. Allodynia is due in part to the sensitization of articular nociceptors to mechanical stimuli. These nociceptors respond to noxious mechanical stimuli applied to their terminals via the expression of depolarizing high-threshold mechanosensitive ion channels (MSICs) that convert painful mechanical forces into electrical signals...
September 5, 2017: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/28859093/pulling-mscl-open-via-n-terminal-and-tm1-helices-a-computational-study-towards-engineering-an-mscl-nanovalve
#16
Adam D Martinac, Navid Bavi, Omid Bavi, Boris Martinac
There are great opportunities in the manipulation of bacterial mechanosensitive (MS) ion channels for specific and targeted drug delivery purposes. Recent research has shown that these ion channels have the potential to be converted into nanovalves through clever use of magnetic nanoparticles and magnetic fields. Using a combination of molecular dynamics (MD) simulations and the finite element (FE) modelling, this study investigates the theoretical feasibility of opening the MscL channel (MS channel of large conductance of E...
2017: PloS One
https://www.readbyqxmd.com/read/28779841/ion-channel-conformation-and-oligomerization-assessment-by-site-directed-spin-labeling-and-pulsed-epr
#17
Christos Pliotas
Mechanosensitive (MS) ion channels are multimeric integral membrane proteins that respond to increased lipid bilayer tension by opening their nonselective pores to release solutes and relieve increased cytoplasmic pressure. These systems undergo major conformational changes during gating and the elucidation of their mechanism requires a deep understanding of the interplay between lipids and proteins. Lipids are responsible for transmitting lateral tension to MS channels and therefore play a key role in obtaining a molecular-detail model for mechanosensation...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28750206/plant-mechanosensitive-ion-channels-an-ocean-of-possibilities
#18
REVIEW
Debarati Basu, Elizabeth S Haswell
Mechanosensitive ion channels, transmembrane proteins that directly couple mechanical stimuli to ion flux, serve to sense and respond to changes in membrane tension in all branches of life. In plants, mechanosensitive channels have been implicated in the perception of important mechanical stimuli such as osmotic pressure, touch, gravity, and pathogenic invasion. Indeed, three established families of plant mechanosensitive ion channels play roles in cell and organelle osmoregulation and root mechanosensing - and it is likely that many other channels and functions await discovery...
July 24, 2017: Current Opinion in Plant Biology
https://www.readbyqxmd.com/read/28729644/erratum-interaction-of-cochlin-and-mechanosensitive-channel-trek-1-in-trabecular-meshwork-cells-influences-the-regulation-of-intraocular-pressure
#19
Teresia A Carreon, Aida Castellanos, Xavier Gasull, Sanjoy K Bhattacharya
A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper.
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28728825/genetic-diseases-of-piezo1-and-piezo2-dysfunction
#20
S L Alper
Mutations in the genes encoding the mechanosensitive cation channels PIEZO1 and PIEZO2 are responsible for multiple hereditary human diseases. Loss-of-function mutations in the human PIEZO1 gene cause autosomal recessive congenital lymphatic dysplasia. Gain-of-function mutations in the human PIEZO1 gene cause the autosomal dominant hemolytic anemia, hereditary xerocytosis (also known as dehydrated stomatocytosis). Loss-of-function mutations in the human PIEZO2 gene cause an autosomal recessive syndrome of muscular atrophy with perinatal respiratory distress, arthrogryposis, and scoliosis...
2017: Current Topics in Membranes
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