Yaoyao Han, Zijing Zhou, Ruitao Jin, Fei Dai, Yifan Ge, Xisan Ju, Xiaonuo Ma, Sitong He, Ling Yuan, Yingying Wang, Wei Yang, Xiaomin Yue, Zhongwen Chen, Yadong Sun, Ben Corry, Charles D Cox, Yixiao Zhang
OSCA/TMEM63 channels are the largest known family of mechanosensitive channels1-3 , playing critical roles in plant4-7 and mammalian8,9 mechanotransduction. Here we determined 44 cryogenic electron microscopy structures of OSCA/TMEM63 channels in different environments to investigate the molecular basis of OSCA/TMEM63 channel mechanosensitivity. In nanodiscs, we mimicked increased membrane tension and observed a dilated pore with membrane access in one of the OSCA1.2 subunits. In liposomes, we captured the fully open structure of OSCA1...
April 3, 2024: Nature