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https://www.readbyqxmd.com/read/29220796/purine-analogs-targeting-the-guanine-riboswitch-as-potential-antibiotics-against-clostridioides-difficile
#1
Lok-Hang Yan, Antoine Le Roux, Kumaraswamy Boyapelly, Anne-Marie Lamontagne, Marie-Ann Archambault, Frédéric Picard-Jean, David Lalonde-Seguin, Emilie St-Pierre, Rafael J Najmanovich, Louis-Charles Fortier, Daniel Lafontaine, Éric Marsault
Riboswitches recently emerged as possible targets for the development of alternative antimicrobial approaches. Guanine-sensing riboswitches in the bacterial pathogen Clostridioides difficile (formerly known as Clostridium difficile) constitute potential targets based on their involvement in the regulation of basal metabolic control of purine compounds. In this study, we deciphered the structure-activity relationship of several guanine derivatives on the guanine riboswitch and determined their antimicrobial activity...
December 2, 2017: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29220506/a-molecular-portrait-of-de-novo-genes-in-yeasts
#2
Nikolaos N Vakirlis, Alex S Hebert, Dana A Opulente, Guillaume Achaz, Chris Todd Hittinger, Gilles Fischer, Joshua J Coon, Ingrid Lafontaine
New genes, with novel protein functions, can evolve "from scratch" out of intergenic sequences. These de novo genes can integrate the cell's genetic network and drive important phenotypic innovations. Therefore, identifying de novo genes and understanding how the transition from noncoding to coding occurs are key problems in evolutionary biology. However, identifying de novo genes is a difficult task, hampered by the presence of remote homologs, fast evolving sequences and erroneously annotated protein coding genes...
December 6, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/29215748/reliability-and-validity-of-the-experiences-in-close-relationships-scale-12-attachment-dimensions-in-a-clinical-sample-with-eating-disorders
#3
Giorgio A Tasca, Agostino Brugnera, Danielle Baldwin, Samantha Carlucci, Angelo Compare, Louise Balfour, Genevieve Proulx, Mary Gick, Marie-France Lafontaine
OBJECTIVE: Attachment insecurity is a potential risk factor for the development and maintenance of eating disorders (EDs). To date, there are multiple psychometrically sound questionnaires for the evaluation of attachment in both clinical and healthy populations, such as the Experience in Close Relationships (ECR) scale. Composed by two subscales (i.e., attachment anxiety and avoidance), the ECR scale was recently adapted to a shorter, 12-item version (ECR-12). However, a validation of the ECR-12 among patients with EDs is still lacking...
December 7, 2017: International Journal of Eating Disorders
https://www.readbyqxmd.com/read/29162725/src-homology-2-domains-enhance-tyrosine-phosphorylation-in-vivo-by-protecting-binding-sites-in-their-target-proteins-from-dephosphorylation
#4
Joshua A Jadwin, Timothy G Curran, Adam T Lafontaine, Forest M White, Bruce J Mayer
Phosphotyrosine (pY)-dependent signaling is critical for many cellular processes. It is highly dynamic, as signal output depends not only on phosphorylation and dephosphorylation rates, but also on the rates of binding and dissociation of effectors containing phosphotyrosine-dependent binding modules such as Src Homology 2 (SH2) and phosphotyrosine-binding (PTB) domains. Previous in vitro studies suggested that binding of SH2 and PTB domains can enhance protein phosphorylation by protecting the sites bound by these domains from phosphatase-mediated dephosphorylation...
November 21, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29157742/phase-1-trial-evaluating-safety-of-pulmonary-artery-sealing-with-ultrasonic-energy-in-vats-lobectomy
#5
Eric Goudie, Ricardo L Oliveira, Vicky Thiffault, Adeline Jouquan, Edwin Lafontaine, Pasquale Ferraro, Moishe Liberman
BACKGROUND: Energy-sealing devices may be useful to divide small pulmonary arteries (PAs) during video-assisted thoracoscopic surgery (VATS) lobectomy. We evaluated the safety of PA branch sealing with an ultrasonic energy vessel-sealing device during VATS lobectomy. METHODS: The study consisted of a phase 1 trial. Patients planned to undergo VATS lobectomy were prospectively enrolled. Target sample size was 20 patients. Branches of 7 mm or less were sealed and cut with an ultrasonic energy vessel-sealing device...
November 17, 2017: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/29145795/use-of-immunohistochemistry-to-demonstrate-in-vivo-expression-of-the-burkholderia-mallei-virulence-factor-bpab-during-experimental-glanders
#6
Shawn M Zimmerman, Mackenzie E Long, Jeremy S Dyke, Tomislav P Jelesijevic, Frank Michel, Eric R Lafontaine, Robert J Hogan
Burkholderia mallei causes the highly contagious and debilitating zoonosis glanders, which infects via inhalation or percutaneous inoculation and often culminates in life-threatening pneumonia and sepsis. In humans, glanders is difficult to diagnose and requires prolonged antibiotic therapy with low success rates. No vaccine exists to protect against B. mallei, and there is concern regarding its use as a bioweapon. The authors previously identified the protein BpaB as a potential target for devising therapies due to its role in adherence to host cells and the formation of biofilms in vitro and its contribution to pathogenicity in a mouse model of glanders...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/29081015/dealing-with-treatment-and-transfer-requests-how-pgd-professionals-discuss-ethical-challenges-arising-in-everyday-practice
#7
Melisa Soto-Lafontaine, Wybo Dondorp, Veerle Provoost, Guido de Wert
How do professionals working in pre-implantation genetic diagnosis (PGD) reflect upon their decision making with regard to ethical challenges arising in everyday practice? Two focus group discussions were held with staff of reproductive genetic clinics: one in Utrecht (The Netherlands) with PGD-professionals from Dutch PGD-centres and one in Prague (Czech Republic) with PGD-professionals working in centres in different European countries. Both meetings consisted of two parts, exploring participants' views regarding (1) treatment requests for conditions that may not fulfill traditional indications criteria for PGD, and (2) treatment and transfer requests involving welfare-of-the-child considerations...
October 28, 2017: Medicine, Health Care, and Philosophy
https://www.readbyqxmd.com/read/29035503/comparison-between-short-and-long-echo-time-magnetic-resonance-spectroscopic-imaging-at-3t-and-7t-for-evaluating-brain-metabolites-in-patients-with-glioma
#8
Yan Li, Marisa Lafontaine, Susan Chang, Sarah J Nelson
Three-dimensional proton magnetic resonance spectroscopic imaging (MRSI) is a powerful non-invasive tool for characterizing spatial variations in metabolic profiles for patients with glioma. Metabolic parameters obtained using this technique have been shown to predict treatment response, disease progression, and transformation to a more malignant phenotype. The availability of ultra-high-field MR systems has the potential to improve the characterization of metabolites. The purpose of this study was to compare the metabolite profiles acquired with conventional long echo time (TE) MRSI at 3T with those obtained with short TE MRSI at 3T and 7T in patients with glioma...
October 16, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28991612/long-term-safety-follow-up-of-an-anterior-chamber-angle-supported-phakic-intraocular-lens
#9
Thomas Kohnen, Laura LaFontaine, Russell Andrew
PURPOSE: To report adverse device effects and annualized endothelial cell loss rate for up to 10 years after implantation of the Acrysof L-series Cachet phakic intraocular lens (pIOL). SETTING: Clinical centers in the United States, European Union, and Canada. DESIGN: Nonrandomized clinical trial. METHODS: After implantation of the pIOL, the endothelial cell density (ECD) at follow-up evaluations was compared with the 6-month postoperative baseline...
September 2017: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/28944627/difficulties-related-to-self-care-among-people-living-with-type-2-diabetes-a-narrative-literature-review-based-on-audulv-asplund-and-norbergh%C3%A2-s-model
#10
Sarah Lafontaine, Edith Ellefsen
Introduction : self-care activities require a lot of adaptation from people living with type 2 diabetes.Context : existing interventions to support self-care are often determined by a conventional vision focused on managing the disease.Objective : this article aims to present the difficulties of people living with type 2 diabetes in relation with the Audulv, Asplund and Norbergh self-care integration model.Method : a narrative review of the literature on self-care in adults with type 2 diabetes was conducted on the CINAHL, MEDLINE, PsycINFO, PASCAL and FRANÇIS databases...
April 19, 2017: Recherche en Soins Infirmiers
https://www.readbyqxmd.com/read/28922789/comprehensive-intrametastatic-immune-quantification-and-major-impact-of-immunoscore-on-survival
#11
Bernhard Mlecnik, Marc Van den Eynde, Gabriela Bindea, Sarah E Church, Angela Vasaturo, Tessa Fredriksen, Lucie Lafontaine, Nacilla Haicheur, Florence Marliot, Daphné Debetancourt, Géraldine Pairet, Anne Jouret-Mourin, Jean-Francois Gigot, Catherine Hubert, Etienne Danse, Cristina Dragean, Javier Carrasco, Yves Humblet, Viia Valge-Archer, Anne Berger, Franck Pagès, Jean-Pascal Machiels, Jérôme Galon
Background: This study assesses how the metastatic immune landscape is impacting the response to treatment and the outcome of colorectal cancer (CRC) patients. Methods: Complete curative resection of metastases (n = 441) was performed for two patient cohorts (n = 153). Immune densities were quantified in the center and invasive margin of all metastases. Immunoscore and T and B cell (TB) score were analyzed in relation to radiological and pathological responses and patient's disease-free (DFS) and overall survival (OS) using multivariable Cox proportional hazards models...
January 1, 2018: Journal of the National Cancer Institute
https://www.readbyqxmd.com/read/28904332/identification-of-sites-of-2-o-methylation-vulnerability-in-human-ribosomal-rnas-by-systematic-mapping
#12
Sunny Sharma, Virginie Marchand, Yuri Motorin, Denis L J Lafontaine
Ribosomal RNA modifications are important in optimizing ribosome function. Sugar 2'-O-methylation performed by fibrillarin-associated box C/D antisense guide snoRNAs impacts all steps of translation, playing a role in disease etiology (cancer). As it renders adjacent phosphodiester bonds resistant to alkaline treatment, 2'-O-methylation can be monitored qualitatively and quantitatively by applying next-generation sequencing to fragments of randomly cleaved RNA. We remapped all sites of 2'-O-methylation in human rRNAs in two isogenic diploid cell lines, one producing and one not producing the antitumor protein p53...
September 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28827772/a-three-enzyme-system-to-generate-the-strychnos-alkaloid-scaffold-from-a-central-biosynthetic-intermediate
#13
Evangelos C Tatsis, Inês Carqueijeiro, Thomas Dugé de Bernonville, Jakob Franke, Thu-Thuy T Dang, Audrey Oudin, Arnaud Lanoue, Florent Lafontaine, Anna K Stavrinides, Marc Clastre, Vincent Courdavault, Sarah E O'Connor
Monoterpene indole alkaloids comprise a diverse family of over 2000 plant-produced natural products. This pathway provides an outstanding example of how nature creates chemical diversity from a single precursor, in this case from the intermediate strictosidine. The enzymes that elicit these seemingly disparate products from strictosidine have hitherto been elusive. Here we show that the concerted action of two enzymes commonly involved in natural product metabolism-an alcohol dehydrogenase and a cytochrome P450-produces unexpected rearrangements in strictosidine when assayed simultaneously...
August 22, 2017: Nature Communications
https://www.readbyqxmd.com/read/28755833/serum-antibody-response-to-moraxella-catarrhalis-proteins-in-stringently-defined-otitis-prone-children
#14
Dabin Ren, Anthony L Almudevar, Timothy F Murphy, Eric R Lafontaine, Anthony A Campagnari, Nicole Luke-Marshall, Michael E Pichichero
BACKGROUND: Moraxella catarrhalis (Mcat) is a frequent pathogen of acute otitis media (AOM) in young children. Here we prospectively assessed naturally-induced serum antibodies to four Mcat vaccine candidate proteins in stringently defined otitis prone (sOP) and non-otitis prone (NOP) children age 6-36months old following nasopharyngeal (NP) colonization, at onset of AOM and convalescence from AOM. METHODS: Serum IgG and IgM antibody against recombinant Mcat proteins, oligopeptide permease A (OppA), outer membrane protein (OMP) CD, hemagglutinin (Hag), and PilA clade 2 (PilA2), were quantitated by ELISA...
July 26, 2017: Vaccine
https://www.readbyqxmd.com/read/28746998/bioaccumulation-distribution-and-elimination-of-chlordecone-in-the-giant-freshwater-prawn-macrobrachium-rosenbergii-field-and-laboratory-studies
#15
Anne Lafontaine, Eric Gismondi, Nathalie Dodet, Célia Joaquim-Justo, Céline Boulangé-Lecomte, Fanny Caupos, Soazig Lemoine, Laurent Lagadic, Joëlle Forget-Leray, Jean-Pierre Thomé
Chlordecone is a persistent organochlorine pesticide that has been widely used in Guadeloupe (French West Indies) to control the banana weevil Cosmopolites sordidus from 1972 to 1993. A few years after its introduction, widespread contamination of soils, rivers, wild animals and aquatic organisms was reported. Although high chlordecone concentrations have been reported in several crustacean species, its uptake, internal distribution, and elimination in aquatic species have never been described. This study aimed at investigating the accumulation and tissue distribution of chlordecone in the giant freshwater prawn Macrobrachium rosenbergii, using both laboratory (30 days exposure) and field (8 months exposure) approaches...
October 2017: Chemosphere
https://www.readbyqxmd.com/read/28701520/ligand-recognition-and-helical-stacking-formation-are-intimately-linked-in-the-sam-i-riboswitch-regulatory-mechanism
#16
Anne-Marie Dussault, Audrey Dubé, Frédéric Jacques, Jonathan P Grondin, Daniel A Lafontaine
Riboswitches are noncoding mRNA elements that control gene expression by altering their structure upon metabolite binding. Although riboswitch crystal structures provide detailed information about RNA-ligand interactions, little knowledge has been gathered to understand how riboswitches modulate gene expression. Here, we study the molecular recognition mechanism of the S-adenosylmethionine SAM-I riboswitch by characterizing the formation of a helical stacking interaction involving the ligand-binding process...
October 2017: RNA
https://www.readbyqxmd.com/read/28650772/l-analyse-de-contribution-pour-%C3%A3-valuer-l-impact-de-la-d%C3%A3-marche-%C3%A3-is-sur-les-processus-d%C3%A3-cisionnels-municipaux-un-choix-m%C3%A3-thodologique-int%C3%A3-ressant
#17
Kareen Nour, Mariève M Lafontaine, Astrid Brousselle, Pernelle Smits, Jean-Marie Buregeya, Julie Loslier, Jean-Louis Denis
La pertinence de l'évaluation d'impact sur la santé (ÉIS) pour promouvoir le développement de politiques publiques favorables à la santé au sein des municipalités est de plus en plus reconnue. L'appréciation des effets d'une démarche d'ÉIS sur les processus décisionnels d'acteurs municipaux peut toutefois être difficile en raison de la multitude d'influences sociales, économiques, géographiques et personnelles auxquels ils sont soumis. Dans un tel contexte, l'approche évaluative de l'analyse de contribution (AC) s'avère particulièrement intéressante puisqu'elle permet de documenter les facteurs menant à l'efficacité d'une intervention en tenant compte des éléments du contexte...
June 2017: Global Health Promotion
https://www.readbyqxmd.com/read/28616185/asymmetry-matters-a-genomic-assessment-of-directional-biases-in-gene-flow-between-hybridizing-spruces
#18
Guillaume de Lafontaine, Jean Bousquet
Assessing directional bias in interspecific gene flow might be important in determining the evolutionary trajectory of closely related species pairs. Using a set of 300 single nucleotide polymorphisms (SNPs) having variable propensity to cross species boundary, we evaluated the genomic extent and direction of interspecific gene flow in a progenitor-derivative spruce species pair (black spruce and red spruce). A higher rate of gene flow was found from black spruce toward red spruce purebreds than vice versa...
June 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28570635/probing-planetary-biodiversity-with-dna-barcodes-the-noctuoidea-of-north-america
#19
Reza Zahiri, J Donald Lafontaine, B Christian Schmidt, Jeremy R deWaard, Evgeny V Zakharov, Paul D N Hebert
This study reports the assembly of a DNA barcode reference library for species in the lepidopteran superfamily Noctuoidea from Canada and the USA. Based on the analysis of 69,378 specimens, the library provides coverage for 97.3% of the noctuoid fauna (3565 of 3664 species). In addition to verifying the strong performance of DNA barcodes in the discrimination of these species, the results indicate close congruence between the number of species analyzed (3565) and the number of sequence clusters (3816) recognized by the Barcode Index Number (BIN) system...
2017: PloS One
https://www.readbyqxmd.com/read/28556672/faster-use-and-fewer-failures-with-needle-free-nasal-glucagon-versus-injectable-glucagon-in-severe-hypoglycemia-rescue-a-simulation-study
#20
Jean-François Yale, Hélène Dulude, Marc Egeth, Claude A Piché, Martin Lafontaine, Dolorès Carballo, Rebecca Margolies, Emily Dissinger, Adam R Shames, Nicole Kaplowitz, Michelle Xiaotian Zhang, Shuyu Zhang, Cristina B Guzman
BACKGROUND: During severe hypoglycemic episodes, people with diabetes depend on others to help with treatment. We compared needle-free nasal glucagon and commercially available injectable glucagon for ease of use by caregivers of people with diabetes and by others in treating simulated episodes of severe hypoglycemia. METHODS: Sixteen instructed caregivers and 15 noninstructed acquaintances administered nasal and injectable glucagon to manikins, simulating unconscious people with diabetes during severe hypoglycemia episodes...
July 2017: Diabetes Technology & Therapeutics
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