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https://www.readbyqxmd.com/read/28804852/the-overall-hemostatic-potential-ohp-assay
#1
Jennifer Curnow
The principle of the overall hemostatic potential (OHP) assay is the generation of a fibrin time curve by optical density readings, which represent the balance between fibrin generation, triggered by thrombin or tissue factor, and fibrinolysis, triggered by tissue plasminogen activator (tPA). OHP measures the integrated effect of procoagulant, anticoagulant, and fibrinolytic factors, and OHP assay parameters provide a means of quantifying both the coagulation and fibrinolytic potential of platelet poor plasma...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28803017/technical-note-use-of-an-automated-grooming-brush-by-heifers-and-potential-for-radiofrequency-identification-based-measurements-of-this-behavior
#2
Rachel L Toaff-Rosenstein, Martin Velez, Cassandra B Tucker
Healthy cattle readily use grooming brushes but this behavior subsides when animals become ill. Tracking use of a brush may provide an opportunity for health monitoring, especially if the process could be automated. We assessed how healthy heifers groom themselves on a brush and hypothesized that radiofrequency identification (RFID) could be used to accurately and automatically record this behavior. Angus and Hereford heifers (n = 16) were fitted with 2 ultra-high-frequency RFID ear tags and monitored in groups of 8 while housed in a pen with an electronic brush, video cameras, and 4 RFID antennas...
August 9, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28794548/informed-consent-process-foundation-of-the-researcher-participant-bond
#3
Amrita Sil, Nilay Kanti Das
Consenting to participate in a clinical research study after being properly and correctly informed upholds the basic ethical principle of "autonomy" in human research. The informed consent is a process by which the physician sensitizes the patient about the nature, procedures, risks benefits, treatment schedules, etc of the study in a language that is non-technical and understandable by the study participant. Informed consent document (ICD) has got two parts: the 'Subject Information Sheet' and the 'Informed Consent Form' (ICF); and they have to be approved by the Institutional Ethics Committee (IEC) before administration...
July 2017: Indian Journal of Dermatology
https://www.readbyqxmd.com/read/28777925/a-career-on-both-sides-of-the-atlantic-memoirs-of-a-molecular-plant-pathologist
#4
Nickolas J Panopoulos
This article recounts the experiences that shaped my career as a molecular plant pathologist. It focuses primarily on technical and conceptual developments in molecular phytobacteriology, shares some personal highlights and untold stories that impacted my professional development, and describes the early years of agricultural biotechnology. Writing this article required reflection on events occurring over several decades that were punctuated by a mid-career relocation across the Atlantic. I hope it will still be useful, informative, and enjoyable to read...
August 4, 2017: Annual Review of Phytopathology
https://www.readbyqxmd.com/read/28774726/girardia-dorotocephala-transcriptome-sequence-assembly-and-validation-through-characterization-of-piwi-homologs-and-stem-cell-progeny-markers
#5
Eugene Matthew P Almazan, Sydney L Lesko, Michael P Markey, Labib Rouhana
Planarian flatworms are popular models for the study of regeneration and stem cell biology in vivo. Technical advances and increased availability of genetic information have fueled the discovery of molecules responsible for stem cell pluripotency and regeneration in flatworms. Unfortunately, most of the planarian research performed worldwide utilizes species that are not natural habitants of North America, which limits their availability to newcomer laboratories and impedes their distribution for educational activities...
August 1, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28768609/toward-predicting-social-support-needs-in-online-health-social-networks
#6
Min-Je Choi, Sung-Hee Kim, Sukwon Lee, Bum Chul Kwon, Ji Soo Yi, Jaegul Choo, Jina Huh
BACKGROUND: While online health social networks (OHSNs) serve as an effective platform for patients to fulfill their various social support needs, predicting the needs of users and providing tailored information remains a challenge. OBJECTIVE: The objective of this study was to discriminate important features for identifying users' social support needs based on knowledge gathered from survey data. This study also provides guidelines for a technical framework, which can be used to predict users' social support needs based on raw data collected from OHSNs...
August 2, 2017: Journal of Medical Internet Research
https://www.readbyqxmd.com/read/28765829/transcriptome-of-melanoma-cells-from-two-mouse-models-tyr-nras-q61k-and-tyr-rack1-ha-tyr-nras-q61k
#7
Cécil Campagne, Stéphanie Pons, Diane Esquerre, Jordi Estellé, Emmanuelle Bourneuf, Uwe Maskos, Giorgia Egidy
The transcriptome sequencing of melanoma cells from two mouse models differing in the expression level of the scaffold protein Receptor for activated C kinase (RACK1) are presented. Primary melanoma cells were harvested from Tyr:NRas(Q61K); Pax3(GFP/+) mice, with or without the Tyr:Rack1-HA transgene. Cells were cultured and infected with scramble shRNA or Rack1-targeting shRNA, on technical triplicates of viral infection. Libraries were prepared by selecting polyadenylated mRNAs and RNA Sequencing (RNASeq) was performed...
October 2017: Data in Brief
https://www.readbyqxmd.com/read/28734736/analysis-of-medication-adherence-related-notes-from-a-service-oriented-community-pharmacy
#8
Matthew Witry, Rachel Parry, Randal McDonough, Michael Deninger
BACKGROUND: Medication nonadherence is a significant public health problem. Community pharmacists are positioned to intervene, however, the process is not well understood. OBJECTIVE: To classify and quantify the reasons for nonadherence documented by community pharmacists. METHODS: A retrospective content analysis of pharmacist notes related to nonadherence at a service oriented community pharmacy in the Midwest United States. Notes from the site's dispensing custom documentation software were obtained from September 1, 2014 through February 28, 2015 that were labeled "compliance", either prompted by proportion of days covered calculations or entered as a drug therapy problem...
July 15, 2017: Research in Social & Administrative Pharmacy: RSAP
https://www.readbyqxmd.com/read/28729296/analysis-of-30-spinal-angiograms-falsely-reported-as-normal-in-18-patients-with-subsequently-documented-spinal-vascular-malformations
#9
P Barreras, D Heck, B Greenberg, J-P Wolinsky, C A Pardo, P Gailloud
BACKGROUND AND PURPOSE: The early diagnosis of spinal vascular malformations suffers from the nonspecificity of their clinical and radiologic presentations. Spinal angiography requires a methodical approach to offer a high diagnostic yield. The prospect of false-negative studies is particularly distressing when addressing conditions with a narrow therapeutic window. The purpose of this study was to identify factors leading to missed findings or inadequate studies in patients with spinal vascular malformations...
July 20, 2017: AJNR. American Journal of Neuroradiology
https://www.readbyqxmd.com/read/28713519/common-solvents-for-making-extraction-of-allergenic-proteins-from-plants-pollens-for-prick-tests-and-related-factors-a-technical-review
#10
REVIEW
Hassan Mansouritorghabeh, Farahzad Jabbari-Azad, Abdolreza Varasteh, Mojtaba Sankian, Reza Farid-Hosseini
Collecting information on influencing factors in developing consistent and high-quality extracts results in accurate diagnosis and effective treatment of type I allergy (IgE mediated). Furthermore, considering that a large number of allergens are currently in practice, any attempt to develop a more effective procedure for preparing extract may be useful. Nowadays, different saline solvents, temperature, incubation time, and PH are being incorporated for preparing allergen extracts. The objective of the current study was to clear and address the commonest of solvent buffers and allied conditions for making extracts of pollens of grasses, trees, and weeds...
May 2017: Electronic Physician
https://www.readbyqxmd.com/read/28695665/analysis-of-large-16s-rrna-illumina-data-sets-impact-of-singleton-read-filtering-on-microbial-community-description
#11
Lucas Auer, Mahendra Mariadassou, Michael O'Donohue, Christophe Klopp, Guillermina Hernandez-Raquet
Next-generation sequencing technologies give access to large sets of data, which are extremely useful in the study of microbial diversity based on 16S rRNA gene. However, the production of such large data sets is not only marred by technical biases and sequencing noise but also increases computation time and disc space use. To improve the accuracy of OTU predictions and overcome both computations, storage and noise issues, recent studies and tools suggested removing all single reads and low abundant OTUs, considering them as noise...
July 11, 2017: Molecular Ecology Resources
https://www.readbyqxmd.com/read/28693492/barriers-to-publishing-in-biomedical-journals-perceived-by-a-sample-of-french-researchers-results-of-the-diazepam-study
#12
Martin Duracinsky, Christophe Lalanne, Laurence Rous, Aichata Fofana Dara, Lesya Baudoin, Claire Pellet, Alexandre Descamps, Fabienne Péretz, Olivier Chassany
BACKGROUND: As publishing is essential but competitive for researchers, difficulties in writing and submitting medical articles to biomedical journals are disabling. The DIAzePAM (Difficultés des Auteurs à la Publication d'Articles Médicaux) survey aimed to assess the difficulties experienced by researchers in the AP-HP (Assistance Publique - Hôpitaux de Paris, i.e., Paris Hospitals Board, France), the largest public health institution in Europe, when preparing articles for biomedical journals...
July 10, 2017: BMC Medical Research Methodology
https://www.readbyqxmd.com/read/28682712/-this-is-how-we-work-here-informal-logic-and-social-order-in-primary-health-care-services-in-mexico-city
#13
Nayelhi Itandehui Saavedra, Shoshana Berenzon, Jorge Galván
People who work in health care facilities participate in a shared set of tacit agreements, attitudes, habits, and behaviors that contribute to the functioning of those institutions, but that can also cause conflict. This phenomenon has been addressed tangentially in the study of bureaucratic practices in governmental agencies, but it has not been carefully explored in the specific context of public health care centers. To this end, we analyzed a series of encounters among staff and patients, as well as the situations surrounding the services offered, in public primary care health centers in Mexico City, based on Erving Goffman's concepts of social order, encounter, and situation, and on the concepts of formal and informal logic...
July 2017: Qualitative Health Research
https://www.readbyqxmd.com/read/28678535/a-fluorescence-sedimentation-assay-for-dsdna-antibodies
#14
K Duus, A H Draborg, E Güven, Y M Moreno, S Jacobsen, C T Nielsen, G Houen
The Farr assay is a radioimmunoassay (RIA) for dsDNA antibodies, based on antibody precipitation using ammonium sulphate and quantification using radio-labelled dsDNA. The RIA-Farr assay offers outstanding clinical specificity and sensitivity for systemic lupus erythematosus (SLE) compared to other assays but does also present some disadvantages as it utilizes radioactive-labelled dsDNA and requires high levels of technical expertise for safe handling. Here, a new precipitation assay, 'Fluoro-Farr' assay, is described...
July 5, 2017: Scandinavian Journal of Clinical and Laboratory Investigation
https://www.readbyqxmd.com/read/28674907/protein-multiplexed-immunoassay-analysis-with-r
#15
Edmond J Breen
Plasma samples from 177 control and type 2 diabetes patients collected at three Australian hospitals are screened for 14 analytes using six custom-made multiplex kits across 60 96-well plates. In total 354 samples were collected from the patients, representing one baseline and one end point sample from each patient. R methods and source code for analyzing the analyte fluorescence response obtained from these samples by Luminex Bio-Plex(®) xMap multiplexed immunoassay technology are disclosed. Techniques and R procedures for reading Bio-Plex(®) result files for statistical analysis and data visualization are also presented...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28667995/developments-on-a-sem-based-x-ray-tomography-system-stabilization-scheme-and-performance-evaluation
#16
L A Gomes Perini, P Bleuet, J Filevich, W Parker, B Buijsse, L F Tz Kwakman
Recent improvements in a SEM-based X-ray tomography system are described. In this type of equipment, X-rays are generated through the interaction between a highly focused electron-beam and a geometrically confined anode target. Unwanted long-term drifts of the e-beam can lead to loss of X-ray flux or decrease of spatial resolution in images. To circumvent this issue, a closed-loop control using FFT-based image correlation is integrated to the acquisition routine, in order to provide an in-line drift correction...
June 2017: Review of Scientific Instruments
https://www.readbyqxmd.com/read/28655142/systematic-reviews-guidance-relevant-for-studies-of-older-people
#17
Susan D Shenkin, Jennifer K Harrison, Tim Wilkinson, Richard M Dodds, John P A Ioannidis
Systematic reviews and meta-analyses are increasingly common. This article aims to provide guidance for people conducting systematic reviews relevant to the healthcare of older people. An awareness of these issues will also help people reading systematic reviews to determine whether the results will influence their clinical practice. It is essential that systematic reviews are performed by a team which includes the required technical and clinical expertise. Those performing reviews for the first time should ensure they have appropriate training and support...
June 24, 2017: Age and Ageing
https://www.readbyqxmd.com/read/28633460/guidelines-for-composing-and-assessing-a-paper-on-treatment-of-pain
#18
Nikolai Bogduk, David J Kennedy, Yakov Vorobeychik, Andrew Engel
Authors, readers, and editors share a common focus. Authors want to publish their work. Readers want to see high-quality, new information. Referees and editors serve to ensure that authors provide valid conclusions based on the quality of information that readers want. Common to each of these roles are instructions to authors. However, these are typically written in an uninspiring, legalistic style, as if they are a set of rules that authors must obey if they expect to get published. This renders the instructions boring and oppressive, if not forbidding...
June 17, 2017: Pain Medicine: the Official Journal of the American Academy of Pain Medicine
https://www.readbyqxmd.com/read/28622351/dna-extraction-replicates-improve-diversity-and-compositional-dissimilarity-in-metabarcoding-of-eukaryotes-in-marine-sediments
#19
Anders Lanzén, Katrine Lekang, Inge Jonassen, Eric M Thompson, Christofer Troedsson
Human impact on marine benthic communities has traditionally been assessed using visible morphological traits and has focused on the macrobenthos, whereas the ecologically important organisms of the meio- and microbenthos have received less attention. DNA metabarcoding offers an alternative to this approach and enables a larger fraction of the biodiversity in marine sediments to be monitored in a cost-efficient manner. Although this methodology remains poorly standardised and challenged by biases inherent to rRNA copy number variation, DNA extraction, PCR, and limitations related to taxonomic identification, it has been shown to be semi-quantitative and useful for comparing taxon abundances between samples...
2017: PloS One
https://www.readbyqxmd.com/read/28611731/the-genomic-architecture-of-novel-simulium-damnosum-wolbachia-prophage-sequence-elements-and-implications-for-onchocerciasis-epidemiology
#20
James L Crainey, Jacob Hurst, Poppy H L Lamberton, Robert A Cheke, Claire E Griffin, Michael D Wilson, Cláudia P Mendes de Araújo, María-Gloria Basáñez, Rory J Post
Research interest in Wolbachia is growing as new discoveries and technical advancements reveal the public health importance of both naturally occurring and artificial infections. Improved understanding of the Wolbachia bacteriophages (WOs) WOcauB2 and WOcauB3 [belonging to a sub-group of four WOs encoding serine recombinases group 1 (sr1WOs)], has enhanced the prospect of novel tools for the genetic manipulation of Wolbachia. The basic biology of sr1WOs, including host range and mode of genomic integration is, however, still poorly understood...
2017: Frontiers in Microbiology
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