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https://www.readbyqxmd.com/read/28710348/spatiotemporal-expression-patterns-of-wheat-amino-acid-transporters-reveal-their-putative-roles-in-nitrogen-transport-and-responses-to-abiotic-stress
#1
Yongfang Wan, Robert King, Rowan A C Mitchell, Keywan Hassani-Pak, Malcolm J Hawkesford
Amino acid transporters have roles in amino acid uptake from soil, long-distance transport, remobilization from vegetative tissues and accumulation in grain. Critically, the majority of wheat grain nitrogen is derived from amino acids remobilized from vegetative organs. However, no systematic analysis of wheat AAT genes has been reported to date. Here, 283 full length wheat AAT genes representing 100 distinct groups of homeologs were identified and curated by selectively consolidating IWGSC CSSv2 and TGACv1 Triticum aestivum genome assemblies and reassembling or mapping of IWGSC CSS chromosome sorted reads to fill any gaps...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28709399/draft-sequencing-and-assembly-of-the-genome-of-the-world-s-largest-fish-the-whale-shark-rhincodon-typus-smith-1828
#2
Timothy D Read, Robert A Petit, Sandeep J Joseph, Md Tauqeer Alam, M Ryan Weil, Maida Ahmad, Ravila Bhimani, Jocelyn S Vuong, Chad P Haase, D Harry Webb, Milton Tan, Alistair D M Dove
BACKGROUND: The whale shark (Rhincodon typus) has by far the largest body size of any elasmobranch (shark or ray) species. Therefore, it is also the largest extant species of the paraphyletic assemblage commonly referred to as fishes. As both a phenotypic extreme and a member of the group Chondrichthyes - the sister group to the remaining gnathostomes, which includes all tetrapods and therefore also humans - its genome is of substantial comparative interest. Whale sharks are also listed as an endangered species on the International Union for Conservation of Nature's Red List of threatened species and are of growing popularity as both a target of ecotourism and as a charismatic conservation ambassador for the pelagic ecosystem...
July 14, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28708006/a-near-visual-acuity-test-for-dentists
#3
P Perrin, M Eichenberger, K W Neuhaus, A Lussi
Unimpaired near vision is crucial in dentistry, but appropriate visual tests at dental working distance are not publicly available. The aim of this study was to validate a novel visual triage test for dentists that is easy to use and freely available. The near visual acuity at 300 mm of 106 dental professionals (aged 21-65 years) was assessed with 1) a validated near visual test for scientific purposes miniaturized on a microfilm; 2) an experimental test using a US $5 bill, in which the first five words of each line in the Lincoln Memorial frieze had to be read under a dental operating light...
July 14, 2017: Operative Dentistry
https://www.readbyqxmd.com/read/28699568/evaluation-of-the-informatician-perspective-determining-types-of-research-papers-preferred-by-clinicians
#4
Boshu Ru, Xiaoyan Wang, Lixia Yao
BACKGROUND: To deliver evidence-based medicine, clinicians often reference resources that are useful to their respective medical practices. Owing to their busy schedules, however, clinicians typically find it challenging to locate these relevant resources out of the rapidly growing number of journals and articles currently being published. The literature-recommender system may provide a possible solution to this issue if the individual needs of clinicians can be identified and applied...
July 5, 2017: BMC Medical Informatics and Decision Making
https://www.readbyqxmd.com/read/28699549/how-the-public-uses-social-media-wechat-to-obtain-health-information-in-china-a-survey-study
#5
Xingting Zhang, Dong Wen, Jun Liang, Jianbo Lei
BACKGROUND: On average, 570 million users, 93% in China's first-tier cities, log on to WeChat every day. WeChat has become the most widely and frequently used social media in China, and has been profoundly integrated into the daily life of many Chinese people. A variety of health-related information may be found on WeChat. The objective of this study is to understand how the general public views the impact of the rapidly emerging social media on health information acquisition. METHODS: A self-administered questionnaire was designed, distributed, collected, and analyzed utilizing the online survey tool Sojump...
July 5, 2017: BMC Medical Informatics and Decision Making
https://www.readbyqxmd.com/read/28698708/pope-john-paul-ii-and-the-neurological-standard-for-the-determination-of-death-a-critical-analysis-of-his-address-to-the-transplantation-society
#6
Doyen Nguyen
The introduction of the "brain death" criterion constitutes a significant paradigm shift in the determination of death. The perception of the public at large is that the Catholic Church has formally endorsed this neurological standard. However, a critical reading of the only magisterial document on this subject, Pope John Paul II's 2000 address, shows that the pope's acceptance of the neurological criterion is conditional in that it entails a twofold requirement. It requires that certain medical presuppositions of the neurological standard are fulfilled, and that its philosophical premise coheres with the Church's teaching on the body-soul union...
May 2017: Linacre Quarterly
https://www.readbyqxmd.com/read/28696841/international-multi-site-survey-on-the-use-of-online-support-groups-in-bipolar-disorder
#7
Rita Bauer, Jörn Conell, Tasha Glenn, Martin Alda, Raffaella Ardau, Bernhard T Baune, Michael Berk, Yuly Bersudsky, Amy Bilderbeck, Alberto Bocchetta, Letizia Bossini, Angela M Paredes Castro, Eric Y W Cheung, Caterina Chillotti, Sabine Choppin, Maria Del Zompo, Rodrigo Dias, Seetal Dodd, Anne Duffy, Bruno Etain, Andrea Fagiolini, Miryam Fernández Hernandez, Julie Garnham, John Geddes, Jonas Gildebro, Ana Gonzalez-Pinto, Guy M Goodwin, Paul Grof, Hirohiko Harima, Stefanie Hassel, Chantal Henry, Diego Hidalgo-Mazzei, Vaisnvy Kapur, Girish Kunigiri, Beny Lafer, Erik R Larsen, Ute Lewitzka, Rasmus W Licht, Anne Hvenegaard Lund, Blazej Misiak, Patryk Piotrowski, Scott Monteith, Rodrigo Munoz, Takako Nakanotani, René E Nielsen, Claire O'donovan, Yasushi Okamura, Yamima Osher, Andreas Reif, Philipp Ritter, Janusz K Rybakowski, Kemal Sagduyu, Brett Sawchuk, Elon Schwartz, Ângela M Scippa, Claire Slaney, Ahmad H Sulaiman, Kirsi Suominen, Aleksandra Suwalska, Peter Tam, Yoshitaka Tatebayashi, Leonardo Tondo, Eduard Vieta, Maj Vinberg, Biju Viswanath, Julia Volkert, Mark Zetin, Peter C Whybrow, Michael Bauer
BACKGROUND: Peer support is an established component of recovery from bipolar disorder, and online support groups may offer opportunities to expand the use of peer support at the patient's convenience. Prior research in bipolar disorder has reported value from online support groups. AIMS: To understand the use of online support groups by patients with bipolar disorder as part of a larger project about information seeking. METHODS: The results are based on a one-time, paper-based anonymous survey about information seeking by patients with bipolar disorder, which was translated into 12 languages...
July 11, 2017: Nordic Journal of Psychiatry
https://www.readbyqxmd.com/read/28696422/metagenomic-binning-of-a-marine-sponge-microbiome-reveals-unity-in-defense-but-metabolic-specialization
#8
Beate M Slaby, Thomas Hackl, Hannes Horn, Kristina Bayer, Ute Hentschel
Marine sponges are ancient metazoans that are populated by distinct and highly diverse microbial communities. In order to obtain deeper insights into the functional gene repertoire of the Mediterranean sponge Aplysina aerophoba, we combined Illumina short-read and PacBio long-read sequencing followed by un-targeted metagenomic binning. We identified a total of 37 high-quality bins representing 11 bacterial phyla and two candidate phyla. Statistical comparison of symbiont genomes with selected reference genomes revealed a significant enrichment of genes related to bacterial defense (restriction-modification systems, toxin-antitoxin systems) as well as genes involved in host colonization and extracellular matrix utilization in sponge symbionts...
July 11, 2017: ISME Journal
https://www.readbyqxmd.com/read/28693492/barriers-to-publishing-in-biomedical-journals-perceived-by-a-sample-of-french-researchers-results-of-the-diazepam-study
#9
Martin Duracinsky, Christophe Lalanne, Laurence Rous, Aichata Fofana Dara, Lesya Baudoin, Claire Pellet, Alexandre Descamps, Fabienne Péretz, Olivier Chassany
BACKGROUND: As publishing is essential but competitive for researchers, difficulties in writing and submitting medical articles to biomedical journals are disabling. The DIAzePAM (Difficultés des Auteurs à la Publication d'Articles Médicaux) survey aimed to assess the difficulties experienced by researchers in the AP-HP (Assistance Publique - Hôpitaux de Paris, i.e., Paris Hospitals Board, France), the largest public health institution in Europe, when preparing articles for biomedical journals...
July 10, 2017: BMC Medical Research Methodology
https://www.readbyqxmd.com/read/28692834/using-national-ambient-air-quality-standards-for-fine-particulate-matter-to-assess-regional-wildland-fire-smoke-and-air-quality-management
#10
Don Schweizer, Ricardo Cisneros, Samuel Traina, Teamrat A Ghezzehei, Glenn Shaw
Wildland fire is an important ecological process in the California Sierra Nevada. Personal accounts from pre-20th century describe a much smokier environment than present day. The policy of suppression beginning in the early 20th century and climate change are contributing to increased megafires. We use a single particulate monitoring site at the wildland urban interface to explore impacts from prescribed, managed, and full suppression wildland fires from 2006 to 2015 producing a contextual assessment of smoke impacts over time at the landscape level...
July 7, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28690679/trichoderma-reesei-complete-genome-sequence-repeat-induced-point-mutation-and-partitioning-of-cazyme-gene-clusters
#11
Wan-Chen Li, Chien-Hao Huang, Chia-Ling Chen, Yu-Chien Chuang, Shu-Yun Tung, Ting-Fang Wang
BACKGROUND: Trichoderma reesei (Ascomycota, Pezizomycotina) QM6a is a model fungus for a broad spectrum of physiological phenomena, including plant cell wall degradation, industrial production of enzymes, light responses, conidiation, sexual development, polyketide biosynthesis, and plant-fungal interactions. The genomes of QM6a and its high enzyme-producing mutants have been sequenced by second-generation-sequencing methods and are publicly available from the Joint Genome Institute. While these genome sequences have offered useful information for genomic and transcriptomic studies, their limitations and especially their short read lengths make them poorly suited for some particular biological problems, including assembly, genome-wide determination of chromosome architecture, and genetic modification or engineering...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28690071/plasmodium-vivax-rhomboid-like-protease-1-gene-diversity-in-thailand
#12
Touchchapol Mataradchakul, Chairat Uthaipibull, Francois Nosten, Joel Vega-Rodriguez, Marcelo Jacobs-Lorena, Usa Lek-Uthai
BACKGROUND: Plasmodium vivax infection remains a major public health problem, especially along the Thailand border regions. We examined the genetic diversity of this parasite by analyzing single-nucleotide polymorphisms (SNPs) of the P. vivax rhomboid-like protease 1 gene (Pvrom1) in parasites collected from western (Tak province, Thai-Myanmar border) and eastern (Chanthaburi province, Thai-Cambodia border) regions. METHODS: Data were collected by a cross-sectional survey, consisting of 47 and 45 P...
July 6, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28686566/guidelines-to-statistical-analysis-of-microbial-composition-data-inferred-from-metagenomic-sequencing
#13
Vera Odintsova, Alexander Tyakht, Dmitry Alexeev
Metagenomics, the application of high-throughput DNA sequencing for surveys of environmental samples, has revolutionized our view on the taxonomic and genetic composition of complex microbial communities. An enormous richness of microbiota keeps unfolding in the context of various fields ranging from biomedicine and food industry to geology. Primary analysis of metagenomic reads allows to infer semi-quantitative data describing the community structure. However, such compositional data possess statistical specific properties that are important to be considered during preprocessing, hypothesis testing and interpreting the results of statistical tests...
July 6, 2017: Current Issues in Molecular Biology
https://www.readbyqxmd.com/read/28684066/-quality-of-life-of-patients-carrying-an-urinary-diversion
#14
Y Neuzillet, M Rouprêt
INTRODUCTION: A urinary diversion is more often needed in the context of total cystectomy for bladder cancer. This pathology and this surgical resection alter the quality of life of patients. This article reviews the consequences of urinary diversions on patients' quality of life and ways to minimize them. MATERIAL AND METHOD: A systematic review of the literature search was performed from the databases Medline (NLM, Pubmed) and Embase, focused on the following keywords: "cystectomy"; "urinary diversion"; "quality of life"; "stoma"; "education"...
July 3, 2017: Progrès en Urologie
https://www.readbyqxmd.com/read/28682712/-this-is-how-we-work-here-informal-logic-and-social-order-in-primary-health-care-services-in-mexico-city
#15
Nayelhi Itandehui Saavedra, Shoshana Berenzon, Jorge Galván
People who work in health care facilities participate in a shared set of tacit agreements, attitudes, habits, and behaviors that contribute to the functioning of those institutions, but that can also cause conflict. This phenomenon has been addressed tangentially in the study of bureaucratic practices in governmental agencies, but it has not been carefully explored in the specific context of public health care centers. To this end, we analyzed a series of encounters among staff and patients, as well as the situations surrounding the services offered, in public primary care health centers in Mexico City, based on Erving Goffman's concepts of social order, encounter, and situation, and on the concepts of formal and informal logic...
July 2017: Qualitative Health Research
https://www.readbyqxmd.com/read/28672765/discovery-and-annotation-of-plant-endogenous-target-mimicry-sequences-from-public-transcriptome-libraries-a-case-study-of-prunus-persica
#16
Gökhan Karakülah
Novel transcript discovery through RNA sequencing has substantially improved our understanding of the transcriptome dynamics of biological systems. Endogenous target mimicry (eTM) transcripts, a novel class of regulatory molecules, bind to their target microRNAs (miRNAs) by base pairing and block their biological activity. The objective of this study was to provide a computational analysis framework for the prediction of putative eTM sequences in plants, and as an example, to discover previously un-annotated eTMs in Prunus persica (peach) transcriptome...
June 28, 2017: Journal of Integrative Bioinformatics
https://www.readbyqxmd.com/read/28671256/genome-wide-analysis-of-salinity-stress-induced-dna-methylation-alterations-in-cotton-gossypium-hirsutum-l-using-the-me-dip-sequencing-technology
#17
X K Lu, N Shu, J J Wang, X G Chen, D L Wang, S Wang, W L Fan, X N Guo, L X Guo, W W Ye
Cytosine DNA methylation is a significant form of DNA modification closely associated with gene expression in eukaryotes, fungi, animals, and plants. Although the reference genomes of cotton (Gossypium hirsutum L.) have been publically available, the salinity-stress-induced DNA methylome alterations in cotton are not well understood. Here, we constructed a map of genome-wide DNA methylation characteristics of cotton leaves under salt stress using the methylated DNA immunoprecipitation sequencing method. The results showed that the methylation reads on chromosome 9 were most comparable with those on the other chromosomes, but the greatest changes occurred on chromosome 8 under salt stress...
June 29, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28669431/radiology-online-patient-education-materials-provided-by-major-university-hospitals-do-they-conform-to-nih-and-ama-guidelines
#18
Arpan V Prabhu, Ashley L Donovan, Tudor Crihalmeanu, David R Hansberry, Nitin Agarwal, Sushil Beriwal, Hrishikesh Kale, Matthew Heller
AIMS: The internet creates opportunities for Americans to access medical information about imaging tests and modalities to guide them in their medical decision-making. Owing to health literacy variations in the general population, the American Medical Association and National Institutes of Health recommend patient education resources to be written between the third and seventh grade levels. Our purpose is to quantitatively assess the readability levels of online radiology educational materials, written for the public, in 20 major university hospitals...
May 22, 2017: Current Problems in Diagnostic Radiology
https://www.readbyqxmd.com/read/28669316/screening-for-ptsd-symptoms-in-unaccompanied-refugee-minors-a-test-of-the-cries-8-questionnaire-in-routine-care
#19
Raziye Salari, Cariz Malekian, Linda Linck, Robert Kristiansson, Anna Sarkadi
AIMS: The dramatic increase in the number of refugees in Europe presents a major public health challenge. The limited existing evidence indicates that the mental health needs of refugees are significant; unaccompanied refugee minors (URMs) constitute a particularly vulnerable group. In this study, we aimed to investigate whether a short questionnaire (Children's Revised Impact of Event Scale; CRIES-8) could be used as a screening tool for PTSD symptoms in URMs, 8-18 years old, during their routine health check-up...
June 1, 2017: Scandinavian Journal of Public Health
https://www.readbyqxmd.com/read/28659722/comparison-of-alternative-splicing-junction-detection-tools-using-rna-seq-data
#20
REVIEW
Lizhong Ding, Ethan Rath, Yongsheng Bai
BACKGROUND: Alternative splicing (AS) is a posttranscriptional process that produces differ-ent transcripts from the same gene and is important to produce diverse protein products in response to environmental stimuli. AS occurs at specific sites on the mRNA sequence, some of which have been de-fined. Multiple bioinformatics tools have been developed to detect AS from experimental data. OBJECTIVES: The goal of this review is to help researchers use specific tools to aid their research and to develop new AS detection tools based on these previously established tools...
June 2017: Current Genomics
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