keyword
https://read.qxmd.com/read/38328068/broadly-inhibitory-antibodies-against-severe-malaria-virulence-proteins
#21
Raphael A Reyes, Sai Sundar Rajan Raghavan, Nicholas K Hurlburt, Viola Introini, Ikhlaq Hussain Kana, Rasmus W Jensen, Elizabeth Martinez-Scholze, Maria Gestal-Mato, Cristina Bancells Bau, Monica Lisa Fernández-Quintero, Johannes R Loeffler, James Alexander Ferguson, Wen-Hsin Lee, Greg Michael Martin, Thor G Theander, Isaac Ssewanyana, Margaret E Feeney, Bryan Greenhouse, Sebastiaan Bol, Andrew B Ward, Maria Bernabeu, Marie Pancera, Louise Turner, Evelien M Bunnik, Thomas Lavstsen
Plasmodium falciparum pathology is driven by the accumulation of parasite-infected erythrocytes in microvessels. This process is mediated by the parasite's polymorphic erythrocyte membrane protein 1 (PfEMP1) adhesion proteins. A subset of PfEMP1 variants that bind human endothelial protein C receptor (EPCR) through their CIDRα1 domains is responsible for severe malaria pathogenesis. A longstanding question is whether individual antibodies can recognize the large repertoire of circulating PfEMP1 variants...
January 25, 2024: bioRxiv
https://read.qxmd.com/read/38300956/using-a-mobile-nanopore-sequencing-lab-for-end-to-end-genomic-surveillance-of-plasmodium-falciparum-a-feasibility-study
#22
JOURNAL ARTICLE
Aurel Holzschuh, Anita Lerch, Bakar S Fakih, Safia Mohammed Aliy, Mohamed Haji Ali, Mohamed Ali Ali, Daniel J Bruzzese, Joshua Yukich, Manuel W Hetzel, Cristian Koepfli
Genomic epidemiology holds promise for malaria control and elimination efforts, for example by informing on Plasmodium falciparum genetic diversity and prevalence of mutations conferring anti-malarial drug resistance. Limited sequencing infrastructure in many malaria-endemic areas prevents the rapid generation of genomic data. To address these issues, we developed and validated assays for P. falciparum nanopore sequencing in endemic sites using a mobile laboratory, targeting key antimalarial drug resistance markers and microhaplotypes...
2024: PLOS Glob Public Health
https://read.qxmd.com/read/38287365/molecular-surveillance-of-kelch-13-polymorphisms-in-plasmodium-falciparum-isolates-from-kenya-and-ethiopia
#23
JOURNAL ARTICLE
Brook Jeang, Daibin Zhong, Ming-Chieh Lee, Harrysone Atieli, Delenasaw Yewhalaw, Guiyun Yan
BACKGROUND: Timely molecular surveillance of Plasmodium falciparum kelch 13 (k13) gene mutations is essential for monitoring the emergence and stemming the spread of artemisinin resistance. Widespread artemisinin resistance, as observed in Southeast Asia, would reverse significant gains that have been made against the malaria burden in Africa. The purpose of this study was to assess the prevalence of k13 polymorphisms in western Kenya and Ethiopia at sites representing varying transmission intensities between 2018 and 2022...
January 29, 2024: Malaria Journal
https://read.qxmd.com/read/38283109/unraveling-key-chloroquine-resistance-associated-alleles-among-plasmodium-falciparum-isolates-in-south-darfur-state-sudan-twelve-years-after-drug-withdrawal
#24
JOURNAL ARTICLE
Abdalmoneim M Magboul, Bakri Y M Nour, Abdelhakam G Tamomh, Rashad Abdul-Ghani, Sayed Mustafa Albushra, Hanan Babiker Eltahir
BACKGROUND: Due to the increasing resistance of Plasmodium falciparum to chloroquine (CQ) in Sudan, a shift from CQ to artesunate combined with sulfadoxine/pyrimethamine as a first-line treatment for uncomplicated falciparum malaria was adopted in 2004. This study aimed to determine the frequency distribution of K76T and N86Y mutations in P. falciparum chloroquine resistance transporter ( pfcrt ) and P. falciparum multidrug resistance 1 ( pfmdr1 ) genes as key markers of resistance to CQ among P...
2024: Infection and Drug Resistance
https://read.qxmd.com/read/38270586/a-novel-computational-pipeline-for-var-gene-expression-augments-the-discovery-of-changes-in-the-plasmodium-falciparum-transcriptome-during-transition-from-in-vivo-to-short-term-in-vitro-culture
#25
JOURNAL ARTICLE
Clare Andradi-Brown, Jan Stephan Wichers-Misterek, Heidrun von Thien, Yannick D Höppner, Judith A M Scholz, Helle Hansson, Emma Filtenborg Hocke, Tim Wolf Gilberger, Michael F Duffy, Thomas Lavstsen, Jake Baum, Thomas D Otto, Aubrey J Cunnington, Anna Bachmann
The pathogenesis of severe Plasmodium falciparum malaria involves cytoadhesive microvascular sequestration of infected erythrocytes, mediated by P. falciparum erythrocyte membrane protein 1 (PfEMP1). PfEMP1 variants are encoded by the highly polymorphic family of var genes, the sequences of which are largely unknown in clinical samples. Previously, we published new approaches for var gene profiling and classification of predicted binding phenotypes in clinical P. falciparum isolates (Wichers et al., 2021), which represented a major technical advance...
January 25, 2024: ELife
https://read.qxmd.com/read/38255246/are-pvcrt-o-and-pvmdr1-gene-mutations-associated-with-plasmodium-vivax-chloroquine-resistant-parasites
#26
JOURNAL ARTICLE
Rebecca de Abreu-Fernandes, Natália Ketrin Almeida-de-Oliveira, Aline Rosa de Lavigne Mello, Lucas Tavares de Queiroz, Jacqueline de Aguiar Barros, Bárbara de Oliveira Baptista, Joseli Oliveira-Ferreira, Rodrigo Medeiros de Souza, Lilian Rose Pratt-Riccio, Patrícia Brasil, Cláudio Tadeu Daniel-Ribeiro, Maria de Fátima Ferreira-da-Cruz
(1) Background: Malaria remains a significant global public health issue. Since parasites quickly became resistant to most of the available antimalarial drugs, treatment effectiveness must be constantly monitored. In Brazil, up to 10% of cases of vivax malaria resistant to chloroquine (CQ) have been registered. Unlike P. falciparum , there are no definitive molecular markers for the chemoresistance of P. vivax to CQ. This work aimed to investigate whether polymorphisms in the pvcrt-o and pvmdr1 genes could be used as markers for assessing its resistance to CQ...
January 9, 2024: Biomedicines
https://read.qxmd.com/read/38254700/the-need-for-novel-asexual-blood-stage-malaria-vaccine-candidates-for-plasmodium-falciparum
#27
REVIEW
Eizo Takashima, Hitoshi Otsuki, Masayuki Morita, Daisuke Ito, Hikaru Nagaoka, Takaaki Yuguchi, Ifra Hassan, Takafumi Tsuboi
Extensive control efforts have significantly reduced malaria cases and deaths over the past two decades, but in recent years, coupled with the COVID-19 pandemic, success has stalled. The WHO has urged the implementation of a number of interventions, including vaccines. The modestly effective RTS,S/AS01 pre-erythrocytic vaccine has been recommended by the WHO for use in sub-Saharan Africa against Plasmodium falciparum in children residing in moderate to high malaria transmission regions. A second pre-erythrocytic vaccine, R21/Matrix-M, was also recommended by the WHO on 3 October 2023...
January 12, 2024: Biomolecules
https://read.qxmd.com/read/38236793/molecular-speciation-of-plasmodium-and-multiplicity-of-p-falciparum-infection-in-the-central-region-of-ghana
#28
JOURNAL ARTICLE
Enoch Aninagyei, Dakorah Mavis Puopelle, Isaac Tukwarlba, George Ghartey-Kwansah, Juliana Attoh, Godwin Adzakpah, Desmond Omane Acheampong
Malaria is endemic in the Central region of Ghana, however, the ecological and the seasonal variations of Plasmodium population structure and the intensity of malaria transmission in multiple sites in the region have not been explored. In this cross-sectional study, five districts in the region were involved. The districts were Agona Swedru, Assin Central and Gomoa East (representing the forest zone) and Abura-Asebu-Kwamankese and Cape Coast representing the coastal zone. Systematically, blood samples were collected from patients with malaria...
2024: PLOS Glob Public Health
https://read.qxmd.com/read/38196592/expansion-of-artemisinin-partial-resistance-mutations-and-lack-of-histidine-rich-protein-2-and-3-deletions-in-plasmodium-falciparum-infections-from-rukara-rwanda
#29
Cecile Schreidah, David Giesbrecht, Pierre Gashema, Neeva Young, Tharcisse Munyaneza, Claude Mambo Muvunyi, Kyaw Thwai, Jean-Baptiste Mazarati, Jeffrey Bailey, Jonathan J Juliano, Corine Karema
BACKGROUND: Emerging artemisinin resistance and diagnostic resistance are a threat to malaria control in Africa. Plasmodium falciparum kelch13 (K13) propeller-domain mutations that confer artemisinin partial resistance have emerged in Africa. K13-561H was initially described at a frequency of 7.4% from Masaka in 2014-2015 but not present in nearby Rukara. By 2018, 19.6% of isolates in Masaka and 22% of isolates in Rukara contained the mutation. Longitudinal monitoring is essential to inform control efforts...
December 18, 2023: medRxiv
https://read.qxmd.com/read/38182727/human-leukocyte-antigen-dqa1-04-01-and-rs2040406-variants-are-associated-with-elevated-risk-of-childhood-burkitt-lymphoma
#30
JOURNAL ARTICLE
Zhiwei Liu, Yang Luo, Samuel Kirimunda, Murielle Verboom, Olusegun O Onabajo, Mateus H Gouveia, Martin D Ogwang, Patrick Kerchan, Steven J Reynolds, Constance N Tenge, Pamela A Were, Robert T Kuremu, Walter N Wekesa, Nestory Masalu, Esther Kawira, Tobias Kinyera, Isaac Otim, Ismail D Legason, Hadijah Nabalende, Herry Dhudha, Leona W Ayers, Kishor Bhatia, James J Goedert, Nathan Cole, Wen Luo, Jia Liu, Michelle Manning, Belynda Hicks, Ludmila Prokunina-Olsson, George Chagaluka, W Thomas Johnston, Nora Mutalima, Eric Borgstein, George N Liomba, Steve Kamiza, Nyengo Mkandawire, Collins Mitambo, Elizabeth M Molyneux, Robert Newton, Ann W Hsing, James E Mensah, Anthony A Adjei, Amy Hutchinson, Mary Carrington, Meredith Yeager, Rainer Blasczyk, Stephen J Chanock, Soumya Raychaudhuri, Sam M Mbulaiteye
Burkitt lymphoma (BL) is responsible for many childhood cancers in sub-Saharan Africa, where it is linked to recurrent or chronic infection by Epstein-Barr virus or Plasmodium falciparum. However, whether human leukocyte antigen (HLA) polymorphisms, which regulate immune response, are associated with BL has not been well investigated, which limits our understanding of BL etiology. Here we investigate this association among 4,645 children aged 0-15 years, 800 with BL, enrolled in Uganda, Tanzania, Kenya, and Malawi...
January 5, 2024: Communications Biology
https://read.qxmd.com/read/38178227/in-vitro-delayed-response-to-dihydroartemisinin-of-malaria-parasites-infecting-sickle-cell-erythocytes
#31
JOURNAL ARTICLE
Albert A Gnondjui, Offianan A Toure, Berenger A Ako, Tossea S Koui, Stanislas E Assohoun, Eric A Gbessi, Landry T N'Guessan, Karim Tuo, Sylvain Beourou, Serge-Brice Assi, Francis A Yapo, Ibrahima Sanogo, Ronan Jambou
BACKGROUND: Decreased efficacy of artemisinin-based combination therapy (ACT) for Plasmodium falciparum malaria has been previously reported in patients with sickle cell disease (SCD). The main purpose of this study was to investigate the in vitro susceptibility of isolates to dihydro-artemisinin (DHA) to provide a hypothesis to explain this treatment failure. METHODS: Isolates were collected from patients attending health centres in Abidjan with uncomplicated P...
January 4, 2024: Malaria Journal
https://read.qxmd.com/read/38101534/immune-tolerance-caused-by-repeated-p-falciparum-infection-against-se36-malaria-vaccine-candidate-antigen-and-the-resulting-limited-polymorphism
#32
REVIEW
Nirianne Marie Q Palacpac, Ken J Ishii, Nobuko Arisue, Takahiro Tougan, Toshihiro Horii
The call for second generation malaria vaccines needs not only the identification of novel candidate antigens or adjuvants but also a better understanding of immune responses and the underlying protective processes. Plasmodium parasites have evolved a range of strategies to manipulate the host immune system to guarantee survival and establish parasitism. These immune evasion strategies hamper efforts to develop effective malaria vaccines. In the case of a malaria vaccine targeting the N-terminal domain of P...
December 13, 2023: Parasitology International
https://read.qxmd.com/read/38099304/high-genetic-and-haplotype-diversity-in-vaccine-candidate-pfceltos-but-not-pfrh5-among-malaria-infected-children-in-ibadan-nigeria
#33
JOURNAL ARTICLE
Mary Aigbiremo Oboh, Naemy Asmorom, Catherine Falade, Olusola Ojurongbe, Bolaji N Thomas
Malaria remains a global public health challenge. The disease has a great impact in sub-Saharan Africa among children under five years of age and pregnant women. Malaria control programs targeting the parasite and mosquitoes vectors with combinational therapy and insecticide-treated bednets are becoming obsolete due to the phenomenon of resistance, which is a challenge for reducing morbidity and mortality. Malaria vaccines would be effective alternative to the problem of parasite and insecticide resistance, but focal reports of polymorphisms in malaria candidate antigens have made it difficult to design an effective malaria vaccine...
2023: PeerJ
https://read.qxmd.com/read/38093209/profiles-of-global-mutations-in-the-human-intercellular-adhesion-molecule-1-icam-1-shed-light-on-population-specific-malaria-susceptibility
#34
JOURNAL ARTICLE
Jasmita Gill, Himmat Singh, Amit Sharma
Plasmodium falciparum is responsible for malaria-related morbidity and mortality. PfEMP1 (P. falciparum erythrocyte membrane protein 1) mediates infected erythrocytes adhesion to various surface vascular receptors, including intercellular adhesion molecule-1 (ICAM-1), associating this interaction with severe malaria in several studies. Genetic variation in host ICAM-1 plays a significant role in determining susceptibility to malaria infection via clinical phenotypes such as the ICAM-1Kilifi variant which has been reported to be associated with susceptibility in populations...
December 13, 2023: BMC Genomics
https://read.qxmd.com/read/38087335/amplicon-deep-sequencing-of-five-highly-polymorphic-markers-of-plasmodium-falciparum-reveals-high-parasite-genetic-diversity-and-moderate-population-structure-in-ethiopia
#35
JOURNAL ARTICLE
Abeba Gebretsadik Reda, Tiffany Huwe, Cristian Koepfli, Ashenafi Assefa, Sofonias Kifle Tessema, Alebachew Messele, Lemu Golassa, Hassen Mamo
BACKGROUND: Plasmodium falciparum genetic diversity can add information on transmission intensity and can be used to track control and elimination interventions. METHODS: Dried blood spots (DBS) were collected from patients who were recruited for a P. falciparum malaria therapeutic efficacy trial in three malaria endemic sites in Ethiopia from October to December 2015, and November to December 2019. qPCR-confirmed infections were subject to amplicon sequencing of polymorphic markers ama1-D3, csp, cpp, cpmp, msp7...
December 12, 2023: Malaria Journal
https://read.qxmd.com/read/38043541/genetic-polymorphism-of-merozoite-surface-protein-1-and-antifolate-resistant-genes-in-plasmodium-falciparum-from-mali-and-niger
#36
JOURNAL ARTICLE
Mahaman Moustapha Lamine, Rabia Maman, Abdoul Aziz Maiga, Ibrahim Maman Laminou
Since 2015, countries in the Sahel region have implemented large-scale seasonal malaria chemoprevention (SMC). However, the mass use of sulfadoxine-pyrimethamine (SP) plus amodiaquine impacts the genetic diversity of malaria parasites and their sensitivity to antimalarials. This study aimed to describe and compare the genetic diversity and SP resistance of Plasmodium falciparum strains in Mali and Niger. We collected 400 blood samples in Mali and Niger from children aged 3-59 months suspected of malaria. Of them, 201 tested positive (Niger, 111, 55...
November 2023: Parasites Hosts Dis
https://read.qxmd.com/read/38031549/comparison-of-molecular-surveillance-methods-to-assess-changes-in-the-population-genetics-of-plasmodium-falciparum-in-high-transmission
#37
JOURNAL ARTICLE
Anita Ghansah, Kathryn E Tiedje, Dionne C Argyropoulos, Christiana O Onwona, Samantha L Deed, Frédéric Labbé, Abraham R Oduro, Kwadwo A Koram, Mercedes Pascual, Karen P Day
A major motivation for developing molecular methods for malaria surveillance is to measure the impact of control interventions on the population genetics of Plasmodium falciparum as a potential marker of progress towards elimination. Here we assess three established methods (i) single nucleotide polymorphism (SNP) barcoding (panel of 24-biallelic loci), (ii) microsatellite genotyping (panel of 12-multiallelic loci), and (iii) var coding (fingerprinting var gene diversity, akin to microhaplotyping) to identify changes in parasite population genetics in response to a short-term indoor residual spraying (IRS) intervention...
2023: Front Parasitol
https://read.qxmd.com/read/38030029/plasmodium-falciparum-population-structure-and-genetic-diversity-of-cell-traversal-protein-for-ookinetes-and-sporozoites-celtos-during-malaria-resurgences-in-dielmo-senegal
#38
JOURNAL ARTICLE
Amélé Nyedzie Wotodjo, Mary Aigbiremo Oboh, Cheikh Sokhna, Nafissatou Diagne, Fatoumata Diène-Sarr, Jean-François Trape, Souleymane Doucouré, Alfred Amambua-Ngwa, Umberto D'Alessandro
The ability to accurately measure the intensity of malaria transmission in areas with low transmission is extremely important to guide elimination efforts. Plasmodium falciparum Cell-traversal protein for ookinetes and sporozoites (PfCelTOS) is an important conserved sporozoite antigen reported as one of the promising malaria vaccine candidates, and could be used to estimate malaria transmission intensity. This study aimed at determining whether the diversity of PfCelTOS gene reflects the changes in malaria transmission that occurred between 2007 and 2014 in Dielmo, a Senegalese village, before and after the implementation of insecticide treated bed nets (ITNs)...
November 27, 2023: Infection, Genetics and Evolution
https://read.qxmd.com/read/38009642/sickle-cell-allele-hbb-rs334-t-is-associated-with-decreased-risk-of-childhood-burkitt-lymphoma-in-east-africa
#39
JOURNAL ARTICLE
Hyokyoung G Hong, Mateus H Gouveia, Martin D Ogwang, Patrick Kerchan, Steven J Reynolds, Constance N Tenge, Pamela A Were, Robert T Kuremu, Walter N Wekesa, Nestory Masalu, Esther Kawira, Tobias Kinyera, Xunde Wang, Jiefu Zhou, Thiago Peixoto Leal, Isaac Otim, Ismail D Legason, Hadijah Nabalende, Herry Dhudha, Mediatrix Mumia, Francine S Baker, Temiloluwa Okusolubo, Leona W Ayers, Kishor Bhatia, James J Goedert, Joshua Woo, Michelle Manning, Nathan Cole, Wen Luo, Belynda Hicks, George Chagaluka, W Thomas Johnston, Nora Mutalima, Eric Borgstein, George N Liomba, Steve Kamiza, Nyengo Mkandawire, Collins Mitambo, Elizabeth M Molyneux, Robert Newton, Amy Hutchinson, Meredith Yeager, Adebowale A Adeyemo, Swee Lay Thein, Charles N Rotimi, Stephen J Chanock, Ludmila Prokunina-Olsson, Sam M Mbulaiteye
Burkitt lymphoma (BL) is an aggressive B-cell lymphoma that significantly contributes to childhood cancer burden in sub-Saharan Africa. Plasmodium falciparum, which causes malaria, is geographically associated with BL, but the evidence remains insufficient for causal inference. Inference could be strengthened by demonstrating that mendelian genes known to protect against malaria-such as the sickle cell trait variant, HBB-rs334(T)-also protect against BL. We investigated this hypothesis among 800 BL cases and 3845 controls in four East African countries using genome-scan data to detect polymorphisms in 22 genes known to affect malaria risk...
November 27, 2023: American Journal of Hematology
https://read.qxmd.com/read/37999623/polymorphism-of-drug-resistance-genes-dhfr-and-dhps-in-plasmodium-falciparum-isolates-among-chinese-migrant-workers-who-returned-from-ghana-in-2013
#40
JOURNAL ARTICLE
Hong Quan, Peng Yu, Kokouvi Kassegne, Hai-Mo Shen, Shen-Bo Chen, Jun-Hu Chen
In 2013, an epidemic of falciparum malaria involving over 820 persons unexpectedly broke out in Shanglin County, Guangxi Zhuang Autonomous Region, China, after a large number of migrant workers returned from Ghana, where they worked as gold miners. Herein, we selected 146 isolates randomly collected from these patients to investigate the resistance characteristics of the parasite to sulfadoxine-pyrimethamine (SP) by screening mutations in the dhfr and dhps genes. All 146 isolates were successfully genotyped for dhps , and only 137 samples were successfully genotyped for dhfr ...
November 19, 2023: Tropical Medicine and Infectious Disease
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