María Celeste Rodríguez, Natalia Ceaglio, Agustina Gugliotta, Javier Villarraza, Ernesto Garay, Antonela Fuselli, Victoria Gastaldi, María Belén Tardivo, Sebastián Antuña, Diego Fontana, Claudio Prieto
Serology assays are essential tools to mitigate the effect of COVID-19, help to identify previous SARS-CoV-2 infections or vaccination, and provide data for surveillance and epidemiologic studies. In this study, we report the production and purification process of the receptor-binding domain (RBD) of SARS-CoV-2 in HEK293 cells, which allowed the design, optimization, and validation of an indirect ELISA (iELISA) for the detection of human anti-RBD antibodies. To find the optimal conditions of this iELISA, a multivariate strategy was performed throughout design of experiments (DoE) and response surface methodology (RSM), one of the main tools of quality by design (QbD) approach...
November 4, 2022: Applied Microbiology and Biotechnology