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Neha Maurya, Mehraj Ud Din Parray, Jitendra Kumar Maurya, Amit Kumar, Rajan Patel
The binding nature of amphiphilic drugs viz. promethazine hydrochloride (PMT) and adiphenine hydrochloride (ADP), with human hemoglobin (Hb) was unraveled by fluorescence, absorbance, time resolved fluorescence, fluorescence resonance energy transfer (FRET) and circular dichroism (CD) spectral techniques in combination with molecular docking and molecular dynamic simulation methods. The steady state fluorescence spectra indicated that both PMT and ADP quenches the fluorescence of Hb through static quenching mechanism which was further confirmed by time resolved fluorescence spectra...
March 11, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Skyler L Jackman, Christopher H Chen, Selmaan N Chettih, Shay Q Neufeld, Iain R Drew, Chimuanya K Agba, Isabella Flaquer, Alexis N Stefano, Thomas J Kennedy, Justine E Belinsky, Keiramarie Roberston, Celia C Beron, Bernardo L Sabatini, Christopher D Harvey, Wade G Regehr
Optical methods of interrogating neural circuits have emerged as powerful tools for understanding how the brain drives behaviors. Optogenetic proteins are widely used to control neuronal activity, while genetically encoded fluorescent reporters are used to monitor activity. These proteins are often expressed by injecting viruses, which frequently leads to inconsistent experiments due to misalignment of expression and optical components. Here, we describe how silk fibroin films simplify optogenetic experiments by providing targeted delivery of viruses...
March 20, 2018: Cell Reports
Daisuke Shimaoka, Kenneth D Harris, Matteo Carandini
Changes in arousal modulate the activity of mouse sensory cortex, but studies in different mice and different sensory areas disagree on whether this modulation enhances or suppresses activity. We measured this modulation simultaneously in multiple cortical areas by imaging mice expressing voltage-sensitive fluorescent proteins (VSFP). VSFP imaging estimates local membrane potential across large portions of cortex. We used temporal filters to predict local potential from running speed or from pupil dilation, two measures of arousal...
March 20, 2018: Cell Reports
Jie Gao, Jun Li, Wen-Chao Geng, Fang-Yuan Chen, Xingchen Duan, Zhe Zheng, Dan Ding, Dong-Sheng Guo
Activatable phototheranostics is highly appealing to meet the demand of precision medicine. However, although displaying the efficacy in construction of activatable photosensitizers (PSs), direct covalent decoration still shows some inevitable issues, such as complex molecular design, tedious synthesis, possible photoactivity changes and potential toxicity. Herein, we propose a novel concept of biomarker displacement activation (BDA) using host-guest strategy. To exemplify BDA, we engineered a PS-loaded nanocarrier by utilizing a macrocyclic amphiphile, where the fluorescence and photoactivity of PS were completely annihilated by the complexation of macrocyclic receptor (OFF state)...
March 21, 2018: Journal of the American Chemical Society
Milton Cordeiro, Ana Rita Otrelo-Cardoso, Dmitri I Svergun, Petr V Konarev, João Carlos Lima, Teresa Santos-Silva, Pedro Viana Baptista
Selective base pairing is the foundation of DNA recognition. Here, we elucidate the molecular and structural details of a FRET-based two-component molecular beacon relying on Steady-state Fluorescence Spectroscopy, Small-angle X-ray Scattering (SAXS), Microscale Thermophoresis (MST) and Differential Electrophoretic Mobility. This molecular beacon was designed to detect the most common fusion sequences causing chronic myeloid leukemia, e14a2 and e13a2. The emission spectra indicate that the self-assembly of the different components of the biosensor occurs sequentially, triggered by the fully complementary target...
March 21, 2018: ACS Chemical Biology
Jesús Mosquera Mosquera, Malou Henriksen-Lacey, Isabel García, Miguel Martínez-Calvo, Jessica Rodriguez Villar, José L Mascareñas, Luis M Liz-Marzán
We describe an approach to regulate the cellular uptake of small gold nanoparticles using supramolecular chemistry. The strategy relies on the functionalization of AuNPs with negatively charged pyranines, which largely hamper their penetration in cells. Cellular uptake can be activated in situ through the addition of cationic covalent cages that specifically recognize the fluorescent pyranine dyes and counterbalance the negative charges. The high selectivity and reversibility of the host-guest recognition activates cellular uptake, even in protein-rich biological media, as well as its regulation by rational addition of either cage or pyranine...
March 21, 2018: Journal of the American Chemical Society
Sebastian Isbaner, Narain Karedla, Izabela Kaminska, Daja Ruhlandt, Mario Raab, Johann Bohlen, Alexey I Chizhik, Ingo Gregor, Philip Tinnefeld, Joerg Enderlein, Roman Tsukanov
Single-molecule localization based super-resolution microscopy has revolutionized optical microscopy and routinely allows for resolving structural details down to a few nanometers. However, there exists a rather large discrepancy between lateral and axial localization accuracy, the latter typically three to five times worse than the former. Here, we use single-molecule Metal Induced Energy Transfer (smMIET) to localize single molecules along the optical axis, and to measure their axial distance with an accuracy of 5 nm...
March 21, 2018: Nano Letters
José Eduardo Pelizon Pelino, Alan Passero, Airton Abrahao Martin, Christine Ann Charles
The objective of this study was to evaluate the in vitro effects, including surface morphological characteristics and chemical elemental properties, of different mouthwash formulations on enamel and dental restorative materials, simulating up to 6 months of daily use. Human enamel samples, hydroxyapatite, composite resin, and ceramic surfaces were exposed to 3 different mouthwashes according to label directions - Listerine® Cool Mint®, Listerine® Total Care, and Listerine® Whitening - versus control (hydroalcohol solution) to simulate daily use for up to 6 months...
March 15, 2018: Brazilian Oral Research
Carlos A Gómez, Jason Sutin, Weicheng Wu, Buyin Fu, Hana Uhlirova, Anna Devor, David A Boas, Sava Sakadžić, Mohammad A Yaseen
Investigating cerebral metabolism in vivo at a microscopic level is essential for understanding brain function and its pathological alterations. The intricate signaling and metabolic dynamics between neurons, glia, and microvasculature requires much more detailed understanding to better comprehend the mechanisms governing brain function and its disease-related changes. We recently demonstrated that pharmacologically-induced alterations to different steps of cerebral metabolism can be distinguished utilizing 2-photon fluorescence lifetime imaging of endogenous reduced nicotinamide adenine dinucleotide (NADH) fluorescence in vivo...
2018: PloS One
Wuxing Dong, Alison C Todd, Angelika Bröer, Sarah R Hulme, Stefan Bröer, Brian Billups
Astrocytes are glial cells that have an intimate physical and functional association with synapses in the brain. One of their main roles is to recycle the neurotransmitters glutamate and gamma-aminobutyric acid (GABA), as a component of the glutamate/GABA-glutamine cycle. They perform this function by sequestering neurotransmitters and releasing glutamine via the neutral amino acid transporter SNAT3. In this way, astrocytes regulate the availability of neurotransmitters and subsequently influence synaptic function...
March 21, 2018: International Journal of Molecular Sciences
Zhijuan Chen, Lingbao Ai, Mam Y Mboge, Robert McKenna, Christopher J Frost, Coy D Heldermon, Susan C Frost
Human cell lines are an important resource for research, and are often used as in vitro models of human diseases. In response to the mandate that all cells should be authenticated, we discovered that the MDA-MB-231 cells that were in use in our lab, did not validate based on the alleles of 9 different markers (STR Profile). We had been using this line as a model of triple negative breast cancer (TNBC) that has the ability to form tumors in immuno-compromised mice. Based on marker analysis, these cells most closely resembled the MCF10A line, which are a near diploid and normal mammary epithelial line...
March 21, 2018: Cancer Biology & Therapy
Koichi Ojima, Emi Ichimura, Takahiro Suzuki, Mika Oe, Susumu Muroya, Takanori Nishimura
Myosin is a major myofibrillar component in skeletal muscles. In myofibrils, approximately 300 myosin molecules form a single thick filament in which there is constant turnover of myosin. Our previous study demonstrated that the myosin replacement rate is reduced by inhibition of protein synthesis (Ojima K. et al. 2015 Am. J. Physiol. Cell Physiol. 309, C669-679); however, additional factors influencing myosin replacement were unknown. Here, we showed that rapid myosin replacement requires heat shock protein 90 (HSP90) activity...
March 21, 2018: American Journal of Physiology. Cell Physiology
Ritika S Joshi, Dipanwita Das Mukherjee, Subhendu Chakrabarty, Ansie Martin, Manojkumar Jadhao, Gopal Chakrabarti, Angshuman Sarkar, Sujit Kumar Ghosh
In the development of small molecule drug candidates, naphthalimide based compounds hold a very important position as potent anti-cancer agents with considerable safety in drug discoveries. Being synthetically and readily accessible, naphthalimide compounds with planar architecture have been developed mostly as DNA targeting intercalators. However, in this article, we demonstrate, for the first time, wherein an unfused naphthalimide-benzothiazole bichromophoric compound 2-(6-chlorobenzo[d] thiazol-2-yl)-1H-benzo[de] isoquinoline-1,3(2H)-dione (CBIQD), designed and synthesized by our group, seem to expand the bioactivity of naphthalimide as anti-mitotic agents also...
March 21, 2018: Journal of Physical Chemistry. B
Sicheng Tang, Yang Zhang, Pravat Dhakal, Laura Ravelo, Cheyenne L Anderson, Kevin M Collins, Françisco M Raymo
A photochemical strategy to encode fluorescence signals in vivo with spatial control was designed around the unique properties of a photoactivatable borondipyrromethene (BODIPY). The photoinduced disconnection of two oxazines, flanking a single BODIPY, in two consecutive steps produces a mixture of three emissive molecules with resolved fluorescence inside polymer beads. The relative amounts and emission intensities of the three fluorophores can be regulated precisely in each bead by adjusting the dose of activating photons to mark individual particles with distinct codes of fluorescence signals...
March 21, 2018: Journal of the American Chemical Society
Dong-Min Kim, Dong Ho Kim, Woong Jung, Kye Young Lee, Dong-Eun Kim
Mutations in epidermal growth factor receptor (EGFR) are known as biomarkers that cause non-small cell lung cancer. Particularly, approximately 45% of non-small cell lung cancer patients possess a deletion in exon 19 of the EGFR gene. A less invasive method for detecting the EGFR mutation is required; thus, we developed a simple polymerase chain reaction (PCR)-based method for detecting EGFR exon 19 deletion by using a quencher-free fluorescent probe DNA and graphene oxide (GO). In the presence of the exon 19 deletion mutation, the fluorophore-labeled DNA probe was designed to be fully complementary to the mutant sequences...
March 21, 2018: Analyst
Michael Coleman Barbour, Kenneth W Gow, Alberto Aliseda
Central venous catheters (CVCs) are routinely filled with a heparin lock while not in use to avoid thrombus formation near the tip. However, heparin leakage is known to occur, and the lock effectiveness remains in question. It was recently shown that convective fluxes from the blood flow in the host vein transport the majority of locking solution away from the tip of hemodialysis catheters immediately after instillation. Combined with the low diffusivity of heparin, this results in concentrations of heparin at the catheter tip that are orders of magnitude lower than at instillation for the majority of the interdialytic phase, diminishing the antithrombotic effectiveness of the lock...
March 16, 2018: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
Xiaochu Ma, Maolin Lu, Jason Gorman, Daniel S Terry, Xinyu Hong, Zhou Zhou, Hong Zhao, Roger B Altman, James Arthos, Scott C Blanchard, Peter D Kwong, James B Munro, Walther Mothes
HIV-1 entry into cells requires binding of the viral envelope glycoprotein (Env) to receptor CD4 and coreceptor. Imaging of individual Env molecules on native virions shows Env trimers to be dynamic, spontaneously transitioning between three distinct well-populated conformational states: a pre-triggered Env (State 1), a default intermediate (State 2) and a three-CD4-bound conformation (State 3), which can be stabilized by binding of CD4 and coreceptor-surrogate antibody 17b. Here, using single-molecule Fluorescence Resonance Energy Transfer (smFRET), we show the default intermediate configuration to be asymmetric, with individual protomers adopting distinct conformations...
March 21, 2018: ELife
Lucie Matouskova, Michal Ackermann, Jana Horakova, Lukas Capek, Petr Henys, Jiri Safka
The study investigates the potential for producing medical components via Selective Laser Melting technology (SLM). The material tested consisted of the biocompatible titanium alloy Ti6Al4V. The research involved the testing of laboratory specimens produced using SLM technology both in vitro and for surface roughness. The aim of the research was to clarify whether SLM technology affects the cytocompatibility of implants and, thus, whether SLM implants provide suitable candidates for medical use following zero or minimum post-fabrication treatment...
March 21, 2018: Expert Review of Medical Devices
Christian A Juillerat, Emily E Moore, Theodore Besmann, Hans-Conrad Zur Loye
Single crystals of two new uranyl phosphates, A6 [(UO2 )7 O4 (PO4 )4 ] (A = Cs, Rb), featuring cation-cation interactions (CCIs) rarely observed in uranium(VI) compounds were synthesized by molten flux methods. This structure crystallizes in the triclinic space group P1̅ with lattice parameters, a = 9.2092(4) Å, b = 9.8405(4) Å, c = 10.1856(5) Å, α = 92.876(2)°, β = 95.675(2)°, and γ = 93.139(2)° for A = Cs and a = 9.2166(9) Å, b = 9.3771(10) Å, c = 10.1210(11) Å, α = 89.981(4)°, β = 96.136(4)°, and γ = 92...
March 21, 2018: Inorganic Chemistry
Mei-Jiao Liu, Juan Yuan, Jin Tao, Yi-Quan Zhang, Cai-Ming Liu, Hui-Zhong Kou
Three new dysprosium(III) complexes [Dy2 (HL1 -o)2 (L1 )(NO3 )3 ][Dy(NO3 )5 ]·1.5ACE·0.5Et2 O (1), [Dy(L1 )3 ]·2.5MeOH·MeCN (2), and [Dy(L2 )3 ]·MeOH·MeCN (3) (HL1 = rhodamine B salicylaldehyde hydrazine, HL2 = rhodamine B 3-methylsalicylaldehyde hydrazine) were synthesized and characterized. Purple complex 1 contains two ring-open ligands HL1 -o and shows fluorescence of the rhodamine amide moiety, whereas yellow complexes 2 and 3 are comprised of ring-close ligands (L1/2 )- and display fluorescence of the salicylaldehyde Schiff base part...
March 21, 2018: Inorganic Chemistry
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