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https://www.readbyqxmd.com/read/28918549/loss-of-lhci-system-affects-lhcii-re-distribution-between-thylakoid-domains-upon-state-transitions
#1
Mauro Bressan, Roberto Bassi, Luca Dall'Osto
LHCI, the peripheral antenna system of Photosystem I, includes four light-harvesting proteins (Lhca1-Lhca4) in higher plants, all of which are devoid in the Arabidopsis thaliana knock-out mutant ΔLhca. PSI absorption cross-section was reduced in the mutant, thus affecting the redox balance of the photosynthetic electron chain and resulting in a more reduced PQ with respect to the wild type. ΔLhca plants developed compensatory response by enhancing LHCII binding to PSI. However, the amplitude of state transitions, as measured from changes of chlorophyll fluorescence in vivo, was unexpectedly low than the high level of PSI-LHCII supercomplex established...
September 16, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28918487/clinical-risk-stratification-of-paediatric-renal-transplant-recipients-using-c1q-and-c3d-fixing-of-de-novo-donor-specific-antibodies
#2
Jon Jin Kim, Olivia Shaw, Chloe Martin, George Michaelides, Ramnath Balasubramaniam, Neil J Sebire, Nizam Mamode, Anthony Dorling, Robert Vaughan, Stephen D Marks
INTRODUCTION: We have previously shown that children who developed de novo donor-specific human leukocyte antigen (HLA) antibodies (DSA) had greater decline in allograft function. We hypothesised that patients with complement-activating DSA would have poorer renal allograft outcomes. METHODS: A total of 75 children developed DSA in the original study. The first positive DSA sample was subsequently tested for C1q and C3d fixing. The primary event was defined as 50% reduction from baseline estimated glomerular filtration rate and was analysed using the Kaplan-Meier estimator...
September 16, 2017: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/28918478/an-ibero-american-inter-laboratory-trial-to-evaluate-serological-tests-for-the-detection-of-anti-neospora-caninum-antibodies-in-cattle
#3
Lucía M Campero, Javier Moreno-Gonzalo, María C Venturini, Gastón Moré, Andrea Dellarupe, Magdalena Rambeaud, Ignacio E Echaide, Beatriz Valentini, Carlos M Campero, Dadín P Moore, Dora B Cano, Marcelo Fort, Rinaldo A Mota, Marcos E Serrano-Martínez, Carlos Cruz-Vázquez, Luis M Ortega-Mora, Gema Álvarez-García
We carried out an inter-laboratory trial to compare the serological tests commonly used for the detection of specific Neospora caninum antibodies in cattle in Ibero-American countries. A total of eight laboratories participated from the following countries: Argentina (n = 4), Brazil (n = 1), Peru (n = 1), Mexico (n = 1), and Spain (n = 1). A blind panel of well-characterized cattle sera (n = 143) and sera representative of the target population (n = 351) was tested by seven in-house indirect fluorescent antibody tests (IFATs 1-7) and three enzyme-linked immunosorbent assays (ELISAs 1-3; two in-house and one commercial)...
September 16, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28918472/a-reliable-protocol-for-colorimetric-determination-of-iron-oxide-nanoparticle-uptake-by-cells
#4
Daiana K Deda, Roberta M Cardoso, Mayara K Uchiyama, Christiane Pavani, Sergio H Toma, Mauricio S Baptista, Koiti Araki
Size, shape, and surface properties of superparamagnetic iron oxide nanoparticles (SPIONs) can influence their interaction with biological systems, particularly the incorporation by tumor cells and consequently the biological activity and efficiency in biomedical applications. Several strategies have been used to evaluate cellular uptake of SPIONs. While qualitative methods are generally based on microscopy techniques, quantitative assays are carried out by techniques such as inductively coupled plasma-mass spectrometry and flow cytometry...
September 16, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28918394/apolipoprotein-l1-confers-ph-switchable-ion-permeability-to-phospholipid-vesicles
#5
Jonathan Bruno, Nicola Pozzi, Jonathan Oliva, John C Edwards
Apolipoprotein L1 (ApoL1) is a human serum protein conferring resistance to African trypanosomes, and certain ApoL1 variants increase susceptibility to some progressive kidney diseases. ApoL1 has been hypothesized to function like a pore-forming colicin and been reported to have permeability effects on both intracellular and plasma membranes. Here, to gain insight into how ApoL1 may function in vivo, we used vesicle-based ion-permeability, direct membrane-association, and intrinsic fluorescence to study the activities of purified recombinant ApoL1...
September 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28918375/synthesis-of-fluorescent-dendrimers-with-aggregation-induced-emission-features-through-a-one-pot-multi-component-reaction-and-their-utilization-for-biological-imaging
#6
Weihua Luo, Ruming Jiang, Meiying Liu, Qing Wan, Jianwen Tian, Yuanqing Wen, Qian-Yong Cao, Junfeng Hui, Xiaoyong Zhang, Yen Wei
Hyperbranched polymers have attracted wide research attention owing to their unique topological structure, physicochemical properties and great potential for applications such asadditives, drug delivery, catalysts and nanotechnology. Among these, the polyamidoamine(PAMAM) dendrimers are some of the most important dendrimers. However, the synthesis and biomedical applications of fluorescent PAMAM dendrimers have received only limited attention. In this work, we present a rather effective and convenient approach for synthesis of fluorescent PAMAM dendrimers with aggregation-induced emission (AIE) properties through a one-pot catalyst-free Mannich reaction under rather mild experimental conditions (e...
September 9, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28918374/a-general-strategy-to-fabricate-photonic-crystal-heterostructure-with-programmed-photonic-stopband
#7
Lijing Zhang, Bofan Liu, Jie Wang, Shengyang Tao, Qingfeng Yan
In this paper, we present a general fabrication strategy to achieve the structure control and the flexible photonic stop band regulation of (2+1) D photonic crystal heterostructures (PCHs) by layer-by-layer depositing the annealed colloidal crystal monolayers of different sphere size. The optical properties of the resulting (2+1) DPCHs with different lattice constants were systematically studied and a universal photonic stopband variation rule was proposed, which makes it possible to program any kind of stopband structure as required, such as dual- or multi-stopbands PCH and ultra-wide stopband PCH...
August 24, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28918321/extending-donor-size-in-d-a-%C3%AF-a-organic-dye-for-dye-sensitized-solar-cells-anti-aggregation-and-improving-electron-injection
#8
Yuan-Chun Hu, Wei-Lu Ding, Xing-Liang Peng, Ze-Sheng Li
We have theoretically designed two D-A-π-A dyes 3 and 4 based on the efficient references 1 and 2 by introducing an extra electron donor unit (D2). Via calculating the electronic structures of isolated dyes, we obtain that dyes 3 and 4 possess stronger light-harvesting efficiency imparted by the fluorescence energy transfer of D2 part, maintain comparable lifetime of excited states, and shorten the electron injection time significantly with regard to 1 and 2. Meanwhile, dye 3 positively shifts the edge of virtual states of TiO2 in a larger extent compared to its counterparts...
September 8, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28918256/transgene-is-specifically-and-functionally-expressed-in-retinal-inhibitory-interneurons-in-the-vgat-chr2-eyfp-mouse
#9
Guo-Zhong Xu, Ling-Jie Cui, Ai-Lin Liu, Wei Zhou, Xue Gong, Yong-Mei Zhong, Xiong-Li Yang, Shi-Jun Weng
Ectopic transgene expression in the retina has been reported in various transgenic mice, indicating the importance of characterizing retinal phenotypes. We examined transgene expression in the VGAT-ChR2-EYFP mouse retina by fluorescent immunohistochemistry and electrophysiology, with special emphasis on EYFP localization in retinal neuronal subtypes identified by specific markers. Strong EYFP signals were detected in both the inner and outer plexiform layers. In addition, the ChR2-EYFP fusion protein was also expressed in somata of the great majority of inhibitory interneurons, including horizontal cells and GABAergic and glycinergic amacrine cells...
September 13, 2017: Neuroscience
https://www.readbyqxmd.com/read/28918220/synthesis-and-application-of-a-highly-selective-copper-ions-fluorescent-probe-based-on-the-coumarin-group
#10
Guangjie He, Xiangli Liu, Jinhe Xu, Liguo Ji, Linlin Yang, Aiying Fan, Songjun Wang, Qingzhi Wang
A highly selective copper ions fluorescent probe based on the coumarin-type Schiff base derivative 1 (probe) was produced by condensation reaction between coumarin carbohydrazide and 1H-indazole-3-carbaldehyde. The UV-vis spectroscopy showed that the maximum absorption peak of compound 1 appeared at 439nm. In the presence of Cu(2+) ions, the maximum peak decreased remarkably compared with other physiological important metal ions and a new absorption peak at 500nm appeared. The job's plot experiments showed that complexes of 1:2 binding mode were formed in CH3CN:HEPES (3:2, v/v) solution...
September 13, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28918219/a-highly-selective-fluorescent-chemosensor-for-cn-based-on-a-novel-bis-salamo-type-tetraoxime-ligand
#11
Fei Wang, Lei Gao, Qing Zhao, Yang Zhang, Wen-Kui Dong, Yu-Jie Ding
The optical properties of a novel chemosensor for cyanide anions based on a symmetric bis(salamo)-type ligand (H3L) were investigated by UV-Vis and fluorescence spectroscopy in MeOH/H2O (1:1 v/v) solution. Sensor H3L can selectively sense CN(-) based on prominent color changes among other anions. The chemosensor exhibits an apparent fluorescence enhancement at 482nm to CN(-) which because cyanide ions interact with CN bonds. Combining the corrected Benesi-Hildebrand formula, the binding constant of the formed host-guest complex was calculated as 2...
September 14, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28918081/developmental-alcohol-exposure-impairs-synaptic-plasticity-without-overtly-altering-microglial-function-in-mouse-visual-cortex
#12
Elissa L Wong, Nina M Lutz, Victoria A Hogan, Cassandra E Lamantia, Helene R McMurray, Jason R Myers, John M Ashton, Ania K Majewska
Fetal alcohol spectrum disorder (FASD), caused by gestational ethanol (EtOH) exposure, is one of the most common causes of non-heritable and life-long mental disability worldwide, with no standard treatment or therapy available. While EtOH exposure can alter the function of both neurons and glia, it is still unclear how EtOH influences brain development to cause deficits in sensory and cognitive processing later in life. Microglia play an important role in shaping synaptic function and plasticity during neural circuit development and have been shown to mount an acute immunological response to EtOH exposure in certain brain regions...
September 13, 2017: Brain, Behavior, and Immunity
https://www.readbyqxmd.com/read/28918077/membrane-electro-oxidizer-a-new-hybrid-membrane-system-with-electrochemical-oxidation-for-enhanced-organics-and-fouling-control
#13
Naresh Mameda, Hyung-June Park, Kwang-Ho Choo
The synergistic combination of membrane filtration with advanced oxidation is of particular interest for next-generation wastewater treatment technologies. A membrane electro-oxidizer (MEO) hybridizing a submerged microfilter and an electrochemical cell was developed and investigated for tertiary treatment of secondary industrial (textile) wastewater effluent. Laboratory- and pilot-scale MEO systems were designed and evaluated for treatment efficiency and membrane fouling control. The MEO achieved substantial removal of color (50-90%), turbidity (>90%), and bacteria (>4 log) as well as chemical oxygen demand (13-31%) and 1,4-dioxane (∼25-53%)...
September 5, 2017: Water Research
https://www.readbyqxmd.com/read/28918054/rapid-and-sensitive-detection-of-breast-cancer-cells-in-patient-blood-with-nuclease-activated-probe-technology
#14
Sven Kruspe, David D Dickey, Kevin T Urak, Giselle N Blanco, Matthew J Miller, Karen C Clark, Elliot Burghardt, Wade R Gutierrez, Sneha D Phadke, Sukriti Kamboj, Timothy Ginader, Brian J Smith, Sarah K Grimm, James Schappet, Howard Ozer, Alexandra Thomas, James O McNamara, Carlos H Chan, Paloma H Giangrande
A challenge for circulating tumor cell (CTC)-based diagnostics is the development of simple and inexpensive methods that reliably detect the diverse cells that make up CTCs. CTC-derived nucleases are one category of proteins that could be exploited to meet this challenge. Advantages of nucleases as CTC biomarkers include: (1) their elevated expression in many cancer cells, including cells implicated in metastasis that have undergone epithelial-to-mesenchymal transition; and (2) their enzymatic activity, which can be exploited for signal amplification in detection methods...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28918028/human-dmbt1-derived-cell-penetrating-peptides-for-intracellular-sirna-delivery
#15
Martina Tuttolomondo, Cinzia Casella, Pernille Lund Hansen, Ester Polo, Luciana M Herda, Kenneth A Dawson, Henrik J Ditzel, Jan Mollenhauer
Small interfering RNA (siRNA) is a promising molecule for gene therapy, but its therapeutic administration remains problematic. Among the recently proposed vectors, cell-penetrating peptides show great promise in in vivo trials for siRNA delivery. Human protein DMBT1 (deleted in malignant brain tumor 1) is a pattern recognition molecule that interacts with polyanions and recognizes and aggregates bacteria. Taking advantage of these properties, we investigated whether specific synthetic DMBT1-derived peptides could be used to formulate nanoparticles for siRNA administration...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28918021/in%C3%A2-vitro-selection-of-cell-internalizing-dna-aptamers-in-a-model-system-of-inflammatory-kidney-disease
#16
Glory Ranches, Melanie Lukasser, Herbert Schramek, Andreas Ploner, Taras Stasyk, Gert Mayer, Günter Mayer, Alexander Hüttenhofer
Chronic kidney disease (CKD) is a progressive pathological condition marked by a gradual loss of kidney function. Treatment of CKD is most effective when diagnosed at an early stage and patients are still asymptomatic. However, current diagnostic biomarkers (e.g., serum creatinine and urine albumin) are insufficient for prediction of the pathogenesis of the disease. To address this need, we applied a cell-SELEX (systematic evolution of ligands by exponential enrichment) approach and identified a series of DNA aptamers, which exhibit high affinity and selectivity for cytokine-stimulated cells, resembling some aspects of a CKD phenotype...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28917946/cellular-uptake-and-intracellular-localization-of-poly-acrylic-acid-nanoparticles-in-a-rainbow-trout-oncorhynchus-mykiss-gill-epithelial-cell-line-rtgill-w1
#17
Lindsey C Felix, Van A Ortega, Greg G Goss
The ever-growing production of engineered nanoparticles (NPs) for use in many agricultural, commercial, consumer, and industrial applications will lead to their accidental or intentional release into the environment. Potential routes of environmental exposure include manufacturing or transport spills, disposal of NP-containing products down the drain and/or in landfills, as well as direct usage on agricultural land. Therefore, NPs will inevitably contaminate aquatic environments and interact with resident organisms...
September 7, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28917920/one-step-hydrothermal-synthesis-of-fluorescent-nanocrystalline-cellulose-carbon-dot-hydrogels
#18
Wei Li, Sichun Wang, Ying Li, Chunhui Ma, Zhanhua Huang, Chunsheng Wang, Jian Li, Zhijun Chen, Shouxin Liu
Fluorescent nanocrystalline cellulose/carbon dots (NCC/CDs) hydrogels were successfully prepared by a facile one-step green hydrothermal carbonization process. The properties of NCC/CDs hydrogels prepared at different temperatures were investigated by fluorescence spectroscopy, transmission electron microscopy, nuclear magnetic resonance spectroscopy, X-ray diffraction, and thermogravimetric analysis. NCC/CDs hydrogels with high crystallinity, high thermal stability, and excellent fluorescence properties can be obtained by controlling the hydrothermal treatment temperature...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917916/quantitative-investigations-of-xylose-and-arabinose-substituents-in-hydroxypropylated-and-hydroxyvinylethylated-arabinoxylans
#19
Dominic Lorenz, Anna Knöpfle, Youssef Akil, Bodo Saake
The chemical structures obtained by the modification of arabinoxylans with the cyclic carbonates propylene carbonate (PC) and 4-vinyl-1,3-dioxolan-2-one (VEC) with varying degrees of substitution were investigated. Therefore, a new analytical method was developed that is based on a microwave-assisted hydrolysis of the polysaccharides with trifluoroacetic acid and the reductive amination with 2-aminobenzoic acid. The peak assignment was achieved by HPLC-MS and the carbohydrate derivatives were quantified by HPLC-fluorescence...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917887/a-visible-and-controllable-porphyrin-poly-ethylene-glycol-%C3%AE-cyclodextrin-hydrogel-nanocomposites-system-for-photo-response
#20
Jie Liang, Xia Dong, Chang Wei, Guilei Ma, Tianjun Liu, Deling Kong, Feng Lv
The real-time controlling and tracking of the evolution and status of the hydrogel are important challenges for accurate and precise assessments. In this article, a visible and controllable hydrogel nanocomposites system for photo response was designed and developed based on a thermosensitive porphyrin-poly(ethylene glycol)/α-cyclodextrin hydrogel loaded with multi-walled carbon nanotubes (PPEG-MWNTs/α-CD). The PPEG-MWNTs/α-CD hydrogel was simply self-assembled with a carbon nanotubes dispersed porphyrin-poly(ethylene glycol) solution and an aqueous solution of α-cyclodextrin by homogeneous stirring...
November 1, 2017: Carbohydrate Polymers
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