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https://www.readbyqxmd.com/read/27911396/automated-quantification-and-analysis-of-cell-counting-procedures-using-imagej-plugins
#1
Jacob O'Brien, Heyam Hayder, Chun Peng
The National Institute of Health's ImageJ is a powerful, freely available image processing software suite. ImageJ has comprehensive particle analysis algorithms which can be used effectively to count various biological particles. When counting large numbers of cell samples, the hemocytometer presents a bottleneck with regards to time. Likewise, counting membranes from migration/invasion assays with the ImageJ plugin Cell Counter, although accurate, is exceptionally labor intensive, subjective, and infamous for causing wrist pain...
November 17, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27910954/mal-lys-prediction-of-lysine-malonylation-sites-in-proteins-integrated-sequence-based-features-with-mrmr-feature-selection
#2
Yan Xu, Ya-Xin Ding, Jun Ding, Ling-Yun Wu, Yu Xue
Lysine malonylation is an important post-translational modification (PTM) in proteins, and has been characterized to be associated with diseases. However, identifying malonyllysine sites still remains to be a great challenge due to the labor-intensive and time-consuming experiments. In view of this situation, the establishment of a useful computational method and the development of an efficient predictor are highly desired. In this study, a predictor Mal-Lys which incorporated residue sequence order information, position-specific amino acid propensity and physicochemical properties was proposed...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27908490/y-chromosomal-haplogroup-distribution-in-the-tuzla-canton-of-bosnia-and-herzegovina-a-concordance-study-using-four-different-in-silico-assignment-algorithms-based-on-y-str-data
#3
S Dogan, N Babic, C Gurkan, A Goksu, D Marjanovic, V Hadziavdic
Y-chromosomal haplogroups are sets of ancestrally related paternal lineages, traditionally assigned by the use of Y-chromosomal single nucleotide polymorphism (Y-SNP) markers. An increasingly popular and a less labor-intensive alternative approach has been Y-chromosomal haplogroup assignment based on already available Y-STR data using a variety of different algorithms. In the present study, such in silico haplogroup assignments were made based on 23-loci Y-STR data for 100 unrelated male individuals from the Tuzla Canton, Bosnia and Herzegovina (B&H) using the following four different algorithms: Whit Athey's Haplogroup Predictor, Jim Cullen's World Haplogroup & Haplogroup-I Subclade Predictor, Vadim Urasin's YPredictor and the NevGen Y-DNA Haplogroup Predictor...
November 1, 2016: Homo: Internationale Zeitschrift Für die Vergleichende Forschung Am Menschen
https://www.readbyqxmd.com/read/27907198/a-molecular-approach-applied-to-enteroviruses-surveillance-in-northern-taiwan-2008-2012
#4
Wan-Yu Chung, Pai-Shan Chiang, Shu-Ting Luo, Tzou-Yien Lin, Kuo-Chien Tsao, Min-Shi Lee
Traditional methods for detection and serotyping of enterovirus infections are virus isolation and immunofluorescence assay (VI-IFA), which are labor-intensive and time-consuming. Recently, VP1 gene has been targeted to develop a CODEHOP-based RT-PCR (VP1-CODEHOP) for the same purpose. In this study, we conducted a 5-year enterovirus surveillance comparing the VI-IFA and VP1-CODEHOP tests. Throat swabs were collected from 431 pediatric patients and 208(48%) and 250(58%) were tested positive by the VI-IFA and VP1-CODEHOP tests, respectively...
2016: PloS One
https://www.readbyqxmd.com/read/27906533/walking-working-surfaces-and-personal-protective-equipment-fall-protection-systems-final-rule
#5
(no author information available yet)
OSHA is revising and updating its general industry standards on walking-working surfaces to prevent and reduce workplace slips, trips, and falls, as well as other injuries and fatalities associated with walking-working surface hazards. The final rule includes revised and new provisions addressing, for example, fixed ladders; rope descent systems; fall protection systems and criteria, including personal fall protection systems; and training on fall hazards and fall protection systems. In addition, the final rule adds requirements on the design, performance, and use of personal fall protection systems...
November 18, 2016: Federal Register
https://www.readbyqxmd.com/read/27906038/craniopagus-parasiticus-a-parasitic-head-protruding-from-temporal-area-of-cranium-a-case-report
#6
Wassihun Nega, Meku Damte, Yonas Girma, Getachew Desta, Mengistu Hailemariam
BACKGROUND: Craniopagus parasiticus is rare with an incidence of approximately four to six cases in 10,000,000 births. In our case, the head of the parasitic twin protruded from the temporal area of the normal twin's cranium. The parasitic twin had two deformed lower limbs, of which one was rudimentary, and long bones of the bilateral lower limbs and some pelvic bone. Dissection of the mass of the parasitic twin's body revealed the intestine but no chest organs or abdominal organs. There was a rudimentary labium but no vaginal opening...
December 1, 2016: Journal of Medical Case Reports
https://www.readbyqxmd.com/read/27905938/the-impact-of-epidemics-on-labor-market-identifying-victims-of-the-middle-east-respiratory-syndrome-in-the-korean-labor-market
#7
Ayoung Lee, Joonmo Cho
BACKGROUND: The vulnerability approach suggests that disasters such as epidemics have different effects according not only to physical vulnerability but also to economic class (status). This paper examines the effect of the Middle East Respiratory Syndrome epidemic on the labor market to investigate whether vulnerable groups become more vulnerable due to an interaction between the socio-economic structure and physical risk. METHODS: This paper examines the effect of the Middle East Respiratory Syndrome epidemic on the labor market by considering unemployment status, job status, working hours, reason for unemployment and underemployment status...
December 1, 2016: International Journal for Equity in Health
https://www.readbyqxmd.com/read/27905648/-a-new-fetal-death-classification-system
#8
Alfredo Ovalle, Ariel Fuentes, Valentina Chacón, Carolina Espinoza, Rodrigo González, Manuel Ramos, Jorge Geraldo, Letsy Osses, Elena Kakarieka
BACKGROUND: Stillbirth is the mayor contributor to perinatal mortality. AIM: To report a system for classification of fetal deaths. MATERIAL AND METHODS: Retrospective cohort study of 29,916 births with 258 fetal deaths that occurred in a public hospital. Data were obtained from audit reports of stillbirths. The method for classification “obstetric condition relevant to the death” was applied, based on obstetric and placental pathological findings analyzed exclusively by a single obstetrician and a single pathologist...
August 2016: Revista Médica de Chile
https://www.readbyqxmd.com/read/27904716/is-anterior-bridge-plating-for-mid-shaft-humeral-fractures-a-suitable-option-for-patients-predominantly-involved-in-overhead-activities-a-functional-outcome-study-in-athletes-and-manual-laborers
#9
Abhishek S Mahajan, Young Gun Kim, Jae Hwa Kim, Prashanth D'sa, Azhar Lakhani, Hyun Soo Ok
BACKGROUND: To assess the functional and radiological outcomes of minimally invasive anterior bridge plating (ABP) for mid-shaft humerus fractures in patients predominantly involved in overhead activities (athletes and manual laborers). METHODS: Forty-eight patients fulfilling inclusion criteria were treated with ABP at a level-I trauma center using a 4.5-mm dynamic/locking compression plate and followed for a period of 1 year. Functional outcome was assessed using the Constant, Mayo elbow, and Disabilities of the Arm, Shoulder and Hand (DASH) scores...
December 2016: Clinics in Orthopedic Surgery
https://www.readbyqxmd.com/read/27904638/virtual-reality-and-anxiety-in-primiparous-women-during-episiotomy-repair
#10
Nahid Jahani Shourab, Samira Ebrahimzadeh Zagami, Nahid Golmakhani, Seyed Reza Mazlom, Ali Nahvi, Ferial Pabarja, Mahdi Talebi, Sohaiela Mohamadi Rizi
BACKGROUND: In recent studies, using virtual reality (VR) has been proposed as a nonpharmacological method for anxiety reduction, but until this time, its effects have not been assessed on anxiety during episiotomy repair. This study aimed to determine the effect of audiovisual distraction (VR) on anxiety in primiparous women during episiotomy repair. MATERIALS AND METHODS: This clinical trial was conducted on 30 primigravida from May to July 2012 in the maternity unit of the Omolbanin Hospital, Mashhad city, Iran...
September 2016: Iranian Journal of Nursing and Midwifery Research
https://www.readbyqxmd.com/read/27904588/normal-birth-in-kazakhstan-labor-management-practice-in-accordance-with-world-health-organization-s-recommendations
#11
Aizhan D Kyzayeva, Aigul B Kudamanova
BACKGROUND: Over the last 10 years, the evidential practice has been developing in Kazakhstan. The clinical protocols of diagnostics and treatment were implemented in the country since 2007. We analyzed the normal birth practice in Kazakhstan. MATERIALS AND METHODS: Obstetricians and midwives were subject to the questionnaire survey in four large Maternity Hospitals of Almaty. RESULTS: Totally, 72 midwives and 50 obstetricians took part in the questionnaire survey...
2016: Journal of Research in Medical Sciences: the Official Journal of Isfahan University of Medical Sciences
https://www.readbyqxmd.com/read/27904309/robot-assisted-abdominal-cerclage-during-pregnancy
#12
Burak Zeybek, Amanda Hill, Gulden Menderes, Mostafa A Borahay, Masoud Azodi, Gokhan Sami Kilic
BACKGROUND AND OBJECTIVES: Cervical insufficiency is a difficult condition to diagnose and can lead to preterm birth, miscarriage, or perinatal infant morbidity and mortality. We conducted this retrospective case study and literature review to evaluate the safety and efficacy of robot-assisted abdominal cerclage during pregnancy. METHODS: We conducted a case series and a systematic review that included patients who underwent robot-assisted abdominal cerclage during pregnancy from January 2010 through March 2016...
October 2016: JSLS: Journal of the Society of Laparoendoscopic Surgeons
https://www.readbyqxmd.com/read/27903080/inter-pregnancy-interval-and-adverse-outcomes-evidence-for-an-additional-risk-in-health-disparate-populations
#13
Shyama Appareddy, Jason Pryor, Beth Bailey
OBJECTIVE: Short interpregnancy interval (IPI), <18 months between pregnancies, is a potential cause of adverse delivery and birth outcomes, and may be a particular issue among those with other risks. Our goal was to examine IPI and delivery/infant complications in Tennessee. METHODS: Birth certificate/vital records data included 101,912 women with a previous delivery. IPI groups (<6, 6-12, 12-18, 18-60 months) were compared on outcomes. RESULTS: Thirty-nine percent of the deliveries had IPI <18 months, 9% were <6 months, rates 11% and 27% higher than nationally...
December 1, 2016: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/27902787/effects-of-a-possible-pollinator-crisis-on-food-crop-production-in-brazil
#14
Samuel M A Novais, Cássio A Nunes, Natália B Santos, Ana R D Amico, G Wilson Fernandes, Maurício Quesada, Rodrigo F Braga, Ana Carolina O Neves
Animal pollinators contribute to human food production and security thereby ensuring an important component of human well-being. The recent decline of these agents in Europe and North America has aroused the concern of a potential global pollinator crisis. In order to prioritize efforts for pollinator conservation, we evaluated the extent to which food production depends on animal pollinators in Brazil-one of the world's agriculture leaders-by comparing cultivated area, produced volume and yield value of major food crops that are pollinator dependent with those that are pollinator non-dependent...
2016: PloS One
https://www.readbyqxmd.com/read/27901551/prolonged-second-stage-what-is-the-optimal-length
#15
Alexis C Gimovsky, Vincenzo Berghella
Importance: Prolonged second stage of labor is a clinical dilemma in which controversy exists on whether extending labor in nulliparous women with epidural anesthesia decreases the incidence of cesarean delivery without increasing maternal or neonatal risks. Objective: This narrative review was conducted to assess the current literature on management of and risks associated with a prolonged second stage in nulliparous women with epidurals. Evidence Acquisition: A review of the current literature was performed to evaluate the incidence of cesarean delivery and the maternal and neonatal outcomes...
November 2016: Obstetrical & Gynecological Survey
https://www.readbyqxmd.com/read/27901222/workload-and-associated-factors-a-study-in-maritime-port-in-brazil
#16
Marta Regina Cezar-Vaz, Clarice Alves Bonow, Marlise Capa Verde de Almeida, Cynthia Fontella Sant'Anna, Leticia Silveira Cardoso
Objective: to identify the effect of the mental, physical, temporal, performance, total effort and frustration demands in the overall workload, and in the same way analyze the global burden of port labor and associated factors that contribute most to their decrease or increase. Method: a cross-sectional, quantitative study, developed with 232 dock workers. For data collection, a structured questionnaire with descriptive, occupational, smoking and illicit drug use variables was applied, as well as variables on the load on the tasks undertaken at work, based on the questionnaire NASA Task Load Index...
November 28, 2016: Revista Latino-americana de Enfermagem
https://www.readbyqxmd.com/read/27900752/-parenting-and-nursing-issues-faced-by-parents-during-the-hospitalization-of-their-preterm-infants
#17
Min-Hsueh Weng, Hung-Chieh Chou
Preterm labor is often a sudden event that impacts significantly on the health of the premature infant and on parenting behaviors. The prognosis for premature infants correlates positively with the effectiveness of parenting. Therefore, encouraging parental functions is important. The present paper reviews the literature on the concept of family-centered care in the contexts of premature-infant health problems, assesses the impacts of preterm birth on the parental role, and assesses the influences of parental dysfunction...
December 2016: Hu Li za Zhi the Journal of Nursing
https://www.readbyqxmd.com/read/27900419/measurement-of-giardia-lamblia-adhesion-force-using-an-integrated-microfluidic-assay
#18
Ling Lu, Guo-Xia Zheng, Yu-Suo Yang, Cheng-Yu Feng, Fang-Fang Liu, Yun-Hua Wang
The mechanisms how Giardias attach to the intestinal epithelium remain unclear. None of the methods currently being used to measure the attachment force could provide a continuous nutrition supply and a micro-aerobic atmosphere to the Giardia. Besides, they are all labor-intensive. In the present research, a microfluidic method based on electric circuit analogy was developed. The input fluid flowed through the inlet channel with different lengths and was distributed in four assay chambers. Shear force gradients were generated in chambers, too...
November 30, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27899559/division-of-labor-among-mycobacterium-smegmatis-rnase-h-enzymes-rnase-h1-activity-of-rnha-or-rnhc-is-essential-for-growth-whereas-rnhb-and-rnha-guard-against-killing-by-hydrogen-peroxide-in-stationary-phase
#19
Richa Gupta, Debashree Chatterjee, Michael S Glickman, Stewart Shuman
RNase H enzymes sense the presence of ribonucleotides in the genome and initiate their removal by incising the ribonucleotide-containing strand of an RNA:DNA hybrid. Mycobacterium smegmatis encodes four RNase H enzymes: RnhA, RnhB, RnhC and RnhD. Here, we interrogate the biochemical activity and nucleic acid substrate specificity of RnhA. We report that RnhA (like RnhC characterized previously) is an RNase H1-type magnesium-dependent endonuclease with stringent specificity for RNA:DNA hybrid duplexes. Whereas RnhA does not incise an embedded mono-ribonucleotide, it can efficiently cleave within tracts of four or more ribonucleotides in duplex DNA...
November 28, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27899471/when-science-becomes-too-easy-science-popularization-inclines-laypeople-to-underrate-their-dependence-on-experts
#20
Lisa Scharrer, Yvonne Rupieper, Marc Stadtler, Rainer Bromme
Science popularization fulfills the important task of making scientific knowledge understandable and accessible for the lay public. However, the simplification of information required to achieve this accessibility may lead to the risk of audiences relying overly strongly on their own epistemic capabilities when making judgments about scientific claims. Moreover, they may underestimate how the division of cognitive labor makes them dependent on experts. This article reports an empirical study demonstrating that this "easiness effect of science popularization" occurs when laypeople read authentic popularized science depictions...
November 29, 2016: Public Understanding of Science
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