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pseudomonas keratitis

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https://www.readbyqxmd.com/read/28330899/il-24-promotes-pseudomonas-aeruginosa-keratitis-in-c57bl-6-mouse-corneas
#1
Bing X Ross, Nan Gao, Xinhan Cui, Theodore J Standiford, Jianjiang Xu, Fu-Shin X Yu
The aim of this study was to elucidate the expression and functions of IL-24 in C57BL/6 mouse corneas in response to Pseudomonas aeruginosa infection. Among IL-20R cytokines, only IL-24 was induced at both mRNA and protein levels by infection at early time points. The upregulation of IL-24 was dampened by flagellin pretreatment, which protects the corneas from microbial infection. Time course studies revealed bimodal early and later peaks of IL-24 expression, a pattern shared with suppressor of cytokine signaling (SOCS)3 but not IL-1β or IL-6...
March 22, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28298316/paediatric-infectious-keratitis-a-case-series-of-107-children-presenting-to-a-tertiary-referral-centre
#2
Julia Dutra Rossetto, Kara M Cavuoto, Carla J Osigian, Ta Chen Peter Chang, Darlene Miller, Hilda Capo, Oriel Spierer
BACKGROUND/AIMS: Corneal ulcers can result in severe visual impairment in children. The recent trends of paediatric microbial ulcerative keratitis in the USA are unknown. The purpose of this study is to report the risk factors, microbiological profile and treatment outcomes of paediatric microbial keratitis in South Florida. METHODS: A university-based tertiary eye care centre retrospective case series between 1992 and 2015. Medical records of 107 paediatric patients (age <18 years) with the diagnosis of microbial ulcerative keratitis were analysed...
March 15, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28295043/chlorin-e6-mediated-photodynamic-inactivation-for-multidrug-resistant-pseudomonas-aeruginosa-keratitis-in-mice-in-vivo
#3
Ming-Feng Wu, Mona Deichelbohrer, Thomas Tschernig, Matthias W Laschke, Nóra Szentmáry, Dirk Hüttenberger, Hans-Jochen Foth, Berthold Seitz, Markus Bischoff
Following corneal epithelium scratches, mouse corneas were infected with the multidrug resistant (MDR) P. aeruginosa strain PA54. 24 hours later, 0% (for control group), 0.01%, 0.05% or 0.1% Chlorin e6 (Ce6), a second generation photosensitizer derived from chlorophyll, was combined with red light, for photodynamic inactivation (PDI). 1 hour or 2 days later, entire mouse eyes were enucleated and homogenized for counting colony forming units (CFU) of P. aeruginosa. For comparison, 0.1% Ce6 mediated PDI was started at 12 hours post infection, and 0...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28292273/ocular-bacterial-infections-at-quiha-ophthalmic-hospital-northern-ethiopia-an-evaluation-according-to-the-risk-factors-and-the-antimicrobial-susceptibility-of-bacterial-isolates
#4
Mebrahtu Teweldemedhin, Muthupandian Saravanan, Araya Gebreyesus, Dawit Gebreegziabiher
BACKGROUND: External and intraocular infections can lead to visual impairments, which is a major public health problem. Bacteria are the most frequent pathogens affecting ocular structures; the increasing rate of antimicrobial drug resistance is a worldwide concern. The aim of this study was to determine the occurrence of bacteria in ocular infections, their antimicrobial susceptibility patterns, and risk factors in bacterial ocular infection. METHODS: A hospital based cross-sectional study was conducted from September 2015 to December 2015 at Quiha Ophthalmic Hospital, Tigray, northern Ethiopia...
March 14, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28261183/effect-of-quorum-quenching-lactonase-in-clinical-isolates-of-pseudomonas-aeruginosa-and-comparison-with-quorum-sensing-inhibitors
#5
Assia Guendouze, Laure Plener, Janek Bzdrenga, Pauline Jacquet, Benjamin Rémy, Mikael Elias, Jean-Philippe Lavigne, David Daudé, Eric Chabrière
Pseudomonas aeruginosa is a Gram negative pathogenic bacterium involved in many human infections including otitis, keratitis, pneumonia, and diabetic foot ulcers. P. aeruginosa uses a communication system, referred to as quorum sensing (QS), to adopt a group behavior by synchronizing the expression of certain genes. Among the regulated traits, secretion of proteases or siderophores, motility and biofilm formation are mainly involved in the pathogenicity. Many efforts have been dedicated to the development of quorum sensing inhibitors (QSI) and quorum quenching (QQ) agents to disrupt QS...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28236499/clinical-features-and-microbiological-in-bacterial-keratitis-in-a-tertiary-referral-hospital
#6
J M Ruiz Caro, L Cabrejas, M R de Hoz, D Mingo, S P Duran
OBJECTIVE: To describe the clinical features, bacterial agents, and antibiotic sensitivity of bacterial keratitis in the Ophthalmology Department at the University Hospital Fundación Jiménez Díaz (HUFJD) in Madrid. MATERIALS AND METHODS: A retrospective observational descriptive study using clinical records and reports of corneal scrapings in patients with bacterial keratitis at the HUFJD conducted between 2009 and 2014. RESULTS: In a sample of 160 patients, gram-positive bacteria were the most prevalent with 64...
February 21, 2017: Archivos de la Sociedad Española de Oftalmología
https://www.readbyqxmd.com/read/28217443/virulence-genome-analysis-of-pseudomonas-aeruginosa-vrfpa10-recovered-from-patient-with-scleritis
#7
Nandagopal Murugan, Jambulingam Malathi, Vetrivel Umashankar, Hajib Narahari Rao Madhavan
Infectious keratitis is a major cause of blindness, next to cataract and majority of cases are mainly caused by gram negative bacterium Pseudomonas aeruginosa (P. aeruginosa). In this study, we investigated a P. aeruginosa VRFPA10 genome which exhibited susceptibility to commonly used drugs in vitro but the patient had poor prognosis due to its hyper virulent nature. Genomic analysis of VRFPA10 deciphered multiple virulence factors and P.aeruginosa Genomic Islands (PAGIs) VRFPA10 genome which correlated with hyper virulence nature of the organism...
June 2017: Genomics Data
https://www.readbyqxmd.com/read/28151861/shifting-trends-in-microbial-keratitis-following-penetrating-keratoplasty-in-taiwan
#8
Hung-Chi Chen, Chia-Yi Lee, Hung-Yu Lin, David Hui-Kang Ma, Phil Yeong-Fong Chen, Ching-Hsi Hsiao, Hsin-Chiung Lin, Lung-Kun Yeh, Hsin-Yuan Tan
To investigate the clinical and microbiological profiles from microbial keratitis following penetrating keratoplasty (PKP) in a tertiary referral center in Taiwan, the medical records of 648 consecutive patients (648 eyes) undergoing PKP between January 2003 and December 2007 were retrospectively reviewed. Patients who subsequently sustained microbial keratitis were enrolled and analyzed for potential risk factors, clinical manifestations, microbiological profiles, complications, graft survival, and final visual outcome...
February 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28149778/epidemiological-clinical-and-laboratory-findings-of-infectious-keratitis-at-mansoura-ophthalmic-center-egypt
#9
Amani E Badawi, Dalia Moemen, Nora L El-Tantawy
AIM: To analyze the epidemiological, clinical and laboratory findings of infectious keratitis. METHODS: A retrospective study on cases of infective keratitis, attended our institution from Mar. 2013 to Feb. 2015, was done at Mansoura Ophthalmic Center, Egypt. Corneal scrapings were performed and processed for direct microscopy and culture in appropriate media using standard laboratory protocols. RESULTS: Out of 245 patients enrolled for study, 247 corneal scrapings were obtained...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28129422/antimicrobial-blue-light-therapy-for-infectious-keratitis-ex-vivo-and-in-vivo-studies
#10
Hong Zhu, Irene E Kochevar, Irmgard Behlau, Jie Zhao, Fenghua Wang, Yucheng Wang, Xiaodong Sun, Michael R Hamblin, Tianhong Dai
Purpose: To investigate the effectiveness of antimicrobial blue light (aBL) as an alternative or adjunctive therapeutic for infectious keratitis. Methods: We developed an ex vivo rabbit model and an in vivo mouse model of infectious keratitis. A bioluminescent strain of Pseudomonas aeruginosa was used as the causative pathogen, allowing noninvasive monitoring of the extent of infection in real time via bioluminescence imaging. Quantitation of bacterial luminescence was correlated to colony-forming units (CFU)...
January 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28123711/gaseous-nitric-oxide-for-the-local-treatment-of-bacterial-keratitis-in-mice
#11
Mona Deichelbohrer, Ming-Feng Wu, Berthold Seitz, Stefan Wagenpfeil, Carola Meier, Markus Bischoff, Thomas Tschernig
The successful treatment of severe bacterial keratitis continues to be a challenge in animals and humans. In the present study the aim was to assess gaseous therapy using gaseous nitric oxide (gNO) in a murine model of Pseudomonas aeruginosa keratitis. The cornea of anesthetized mice was mechanically scratched and covered with a bacterial suspension of P. aeruginosa. One day later, the infected eyes were exposed to 200 ppm NO for 30 min. Three to seven days later the mice were sacrificed and the bulbi were obtained and processed for light microscopy...
January 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28092397/update-on-orthokeratology-in-managing-progressive-myopia-in-children-efficacy-mechanisms-and-concerns
#12
Xintong Li, Ilana B Friedman, Norman B Medow, Cheng Zhang
Myopia is an important public health issue, and high myopia may lead to severe complications if left untreated. Orthokeratology lenses, worn overnight to reshape the cornea, are one of many recent modalities used to slow down the progression of myopia in children. This treatment has been proven successful, as evidenced by decreased spherical refractive error and axial length relative to the control at interval follow-up ranging from 6 months to 5 years. In this systematic review, the authors collected published controlled studies that analyzed the efficacy of orthokeratology lens wear and calculated longitudinal relative changes in axial length, revealing a weighted average of -45...
January 13, 2017: Journal of Pediatric Ophthalmology and Strabismus
https://www.readbyqxmd.com/read/28079686/number-of-bacteria-and-time-of-coincubation-with-bacteria-required-for-the-development-of-acanthamoeba-keratitis
#13
Hayate Nakagawa, Takaaki Hattori, Naohito Koike, Tomoko Ehara, Akitomo Narimatsu, Shigeto Kumakura, Tetsuya Matsumoto, Hiroshi Goto
PURPOSE: We hypothesized that bacteria may be a factor contributing to the development of Acanthamoeba keratitis (AK). We investigated interactions between Acanthamoeba and Pseudomonas aeruginosa for the development of keratitis in rabbit corneas. METHODS: Acanthamoeba castellanii (ATCC50492) and P. aeruginosa (PAO-1) were used. Two densities of P. aeruginosa (high, 1 × 10/mL; low, 3 × 10/mL) and 2 durations of coincubation (long, 6 h; short, 2 h) of Acanthamoeba with 1 × 10/mL of P...
March 2017: Cornea
https://www.readbyqxmd.com/read/28079684/the-cif-virulence-factor-gene-is-present-in-isolates-from-patients-with-pseudomonas-aeruginosa-keratitis
#14
Christopher D Bahl, Jessica D St Laurent, R Siva Ganesa Karthikeyan, J Lakshmi Priya, Lalitha Prajna, Michael E Zegans, Dean R Madden
PURPOSE: To determine whether the cif gene is present in pathogenic Pseudomonas aeruginosa isolates from patients with bacterial keratitis at Aravind Eye Hospital, a referral eye care center in southern India, and from corresponding environmental isolates. METHODS: Polymerase chain reaction amplification was performed on strains of P. aeruginosa isolated from ocular infections and environmental soil samples were collected from the area surrounding Aravind Eye Hospital...
March 2017: Cornea
https://www.readbyqxmd.com/read/28002114/treatment-of-pseudomonas-keratitis-by-continuous-infusion-of-topical-antibiotics-with-the-morgan-lens
#15
Mingwu Wang, Whitney A Smith, Joshua K Duncan, Joseph M Miller
PURPOSE: Despite following standard treatment, Pseudomonas keratitis can continue to progress and result in loss of vision or of the eye. Our cases demonstrate that the Morgan Lens can be an effective topical antibiotic delivery vehicle in advanced keratitis. METHODS: Two patients (3 eyes) with Pseudomonas keratitis were included in this report after failing to respond to intense inpatient topical treatment. Because loss of the eyes was imminent, the Morgan Lenses were used for continuous lavage with ceftazidime (50 mg/mL), in conjunction with other conventional treatment...
December 20, 2016: Cornea
https://www.readbyqxmd.com/read/27998732/pathogenesis-of-microbial-keratitis
#16
REVIEW
Sahreena Lakhundi, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Microbial keratitis is a sight-threatening ocular infection caused by bacteria, fungi, and protist pathogens. Epithelial defects and injuries are key predisposing factors making the eye susceptible to corneal pathogens. Among bacterial pathogens, the most common agents responsible for keratitis include Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pneumonia and Serratia species. Fungal agents of corneal infections include both filamentous as well as yeast, including Fusarium, Aspergillus, Phaeohyphomycetes, Curvularia, Paecilomyces, Scedosporium and Candida species, while in protists, Acanthamoeba spp...
March 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27995356/tripartite-motif-8-trim8-positively-regulates-pro-inflammatory-responses-in-pseudomonas-aeruginosa-induced-keratitis-through-promoting-k63-linked-polyubiquitination-of-tak1-protein
#17
Litao Guo, Weili Dong, Xiaoxiao Fu, Jing Lin, Zhijun Dong, Xiaobo Tan, Tiemin Zhang
Pseudomonas aeruginosa (PA)-induced keratitis is a rapidly progressive ocular infectious disease that often leads to inflammatory epithelial edema, stromal infiltration, tissue destruction, corneal ulceration, and vision loss. In this study, we investigate the role of tripartite motif 8 (TRIM8) in regulating the inflammatory process of PA-induced keratitis. The expression of TRIM8 was increased in mouse corneas and in vitro-cultured macrophages after PA infection. Knockdown of the expression of TRIM8 significantly inhibited the activation of NF-κB signaling and decreased the production of pro-inflammatory cytokines both in vivo and in vitro after infected with PA...
April 2017: Inflammation
https://www.readbyqxmd.com/read/27960579/genus-distribution-of-bacteria-and-fungi-associated-with-keratitis-in-a-large-eye-center-located-in-southern-china
#18
Lixia Lin, Weizhong Lan, Bingsheng Lou, Hongmin Ke, Yuanzhe Yang, Xiaofeng Lin, Lingyi Liang
PURPOSE: To investigate the genus distribution of bacteria and fungi associated with keratitis in a large eye center located in Southern China and to compare the results with existing data from other areas in China. METHODS: All results of corneal microbiological examinations from 2009 to 2013 of patients who had been clinically diagnosed with bacterial or fungal keratitis were obtained chronologically and anonymously from the microbiology database at Zhongshan Ophthalmic Center...
April 2017: Ophthalmic Epidemiology
https://www.readbyqxmd.com/read/27928519/antimicrobial-susceptibility-and-minimal-inhibitory-concentration-of-pseudomonas-aeruginosa-isolated-from-septic-ocular-surface-disease-in-different-animal-species
#19
L Leigue, F Montiani-Ferreira, B A Moore
The purpose of this study was to evaluate the antibiotic susceptibility profile of Pseudomonas aeruginosa isolated from different animal species with septic ocular surface disease. Sixteen strains of P. aeruginosa were isolated from different species of animals (dog, cat, horse, penguin and brown bear) with ocular surface diseases such as conjunctivitis, keratocojnuctivits sicca and ulcerative keratitis. These isolates were tested against 11 different antimicrobials agents using the Kirby-Bauer disk-diffusion method...
2016: Open veterinary journal
https://www.readbyqxmd.com/read/27844206/ex-vivo-rabbit-and-human-corneas-as-models-for-bacterial-and-fungal-keratitis
#20
Abigail Pinnock, Nagaveni Shivshetty, Sanhita Roy, Stephen Rimmer, Ian Douglas, Sheila MacNeil, Prashant Garg
PURPOSE: In the study of microbial keratitis, in vivo animal models often require a large number of animals, and in vitro monolayer cell culture does not maintain the three-dimensional structure of the tissues or cell-to-cell communication of in vivo models. Here, we propose reproducible ex vivo models of single- and dual-infection keratitis as an alternative to in vivo and in vitro models. METHODS: Excised rabbit and human corneoscleral rims maintained in organ culture were infected using 10(8) cells of Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans or Fusarium solani...
February 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
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