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pseudomonas keratitis

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https://www.readbyqxmd.com/read/28631638/clinicomicrobiological-profile-of-endophthalmitis-a-10-year-experience-in-a-tertiary-care-center-in-north-india
#1
Gita Satpathy, Niranjan Nayak, Meenakshi Wadhwani, Pradeep Venkwatesh, Atul Kumar, Yograj Sharma, Vishnu Sreenivas
PURPOSE: To determine the clinicomicrobiological profile of infectious agents and their antibiotic susceptibility in different type of endophthalmitis. METHODS: A retrospective review of clinical and microbiological records from January 2001 to December 2010, was performed in 1110 patients diagnosed with different type of endophthalmitis (postoperative, posttraumatic, endogenous and post keratitis) to record the demographic details, clinical presentations; microbiological agents isolated with their antimicrobial sensitivity pattern...
April 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28573109/persistence-of-innate-immune-pathways-in-late-stage-human-bacterial-and-fungal-keratitis-results-from-a-comparative-transcriptome-analysis
#2
Jaya D Chidambaram, Shichina Kannambath, Palepu Srikanthi, Manisha Shah, Prajna Lalitha, Shanmugam Elakkiya, Julien Bauer, Namperumalsamy V Prajna, Martin J Holland, Matthew J Burton
Microbial keratitis (MK) is a major cause of blindness worldwide. Despite adequate antimicrobial treatment, tissue damage can ensue. We compared the human corneal transcriptional profile in late stage MK to normal corneal tissue to identify pathways involved in pathogenesis. Total RNA from MK tissue and normal cadaver corneas was used to determine transcriptome profiles with Illumina HumanHT-12 v4 beadchips. We performed differential expression and network analysis of genes in bacterial keratitis (BK) and fungal keratitis (FK) compared with control (C) samples...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28534320/infectious-keratitis-and-orthokeratology-lens-use-a-systematic-review
#3
REVIEW
Ka Wai Kam, Wing Yung, Gabriel Ka Hin Li, Li Jia Chen, Alvin L Young
PURPOSE: Myopia is a prevalent condition among Asians. Orthokeratology lens has gained popularity as a method of myopia control. This systematic review is to summarize the clinical profile of infectious keratitis in association with orthokeratology lens wear. METHODS: We searched in the PubMed and EMBASE for articles adopting the search strategy "(orthokeratology lens OR orthokeratology) AND (bacterial eye infection OR keratitis OR cornea ulcer OR microbial keratitis OR bacterial keratitis)", from the start date of the databases to August 23, 2016...
May 22, 2017: Infection
https://www.readbyqxmd.com/read/28506643/epithelial-basement-membrane-injury-and-regeneration-modulates-corneal-fibrosis-after-pseudomonas-corneal-ulcers-in-rabbits
#4
Gustavo K Marino, Marcony R Santhiago, Abirami Santhanam, Luciana Lassance, Shanmugapriya Thangavadivel, Carla S Medeiros, Karthikeyan Bose, Kwai Ping Tam, Steven E Wilson
The purpose of this study was to investigate whether myofibroblast-related fibrosis (scarring) after microbial keratitis was modulated by the epithelial basement membrane (EBM) injury and regeneration. Rabbits were infected with Pseudomonas aeruginosa after epithelial scrape injury and the resultant severe keratitis was treated with topical tobramycin. Corneas were analyzed from one to four months after keratitis with slit lamp photos, immunohistochemistry for alpha-smooth muscle actin (α-SMA) and monocyte lineage marker CD11b, and transmission electron microscopy...
May 13, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28484310/a-dot-hybridization-assay-for-the-diagnosis-of-bacterial-keratitis
#5
Po-Chiung Fang, Chun-Chih Chien, Hun-Ju Yu, Ren-Wen Ho, Shin-Ling Tseng, Yu-Hsuan Lai, Ming-Tse Kuo
PURPOSE: To evaluate a bacterial dot hybridization (BDH) assay for the diagnosis of bacterial keratitis (BK). METHODS: Sixty-one qualified corneal scrapings from 61 patients with suspected microbial keratitis were collected consecutively and prospectively. Among the 61 patients, 16 cases were BK and 45 cases were non-BK, including fungal keratitis, viral keratitis, parasitic keratitis, and non-microbial keratitis. Molecular diagnosis of BK in these corneal scrapes was performed using the BDH assay with three universal bacterial probes (PB1, PB2, and PB3) and three genus-specific probes (Aci, Klb, and Psu) to detect Acinetobacter, Klebsiella, and Pseudomonas, respectively...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28469996/signaling-mediated-by-toll-like-receptor-5-sensing-of-pseudomonas-aeruginosa-flagellin-influences-il-1%C3%AE-and-il-18-production-by-primary-fibroblasts-derived-from-the-human-cornea
#6
Maria Del Mar Cendra, Myron Christodoulides, Parwez Hossain
Pseudomonas aeruginosa is the principal cause of bacterial keratitis worldwide and overstimulation of the innate immune system by this organism is believed to contribute significantly to sight loss. In the current study, we have used primary human corneal fibroblast (hCF) cells as an ex vivo model of corneal infection to examine the role of P. aeruginosa flagellum and type three secretion system (TTSS) in inducing inflammasome-associated molecules that trigger IL-1β and IL-18 production during the early stages of the infection...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28359825/bilateral-simultaneous-infective-keratitis
#7
Lai Hiu On Annie, Manotosh Ray
AIM: To analyze the demographics, risk factors, clinical and microbiological characteristics of cases of bilateral simultaneous infective keratitis. METHODS: In this retrospective case series, patients with clinical evidence of bilateral simultaneous infective keratitis were identified from January 1, 2011 to August 31, 2016. Demographics, risk factors, clinical and microbiological characteristics, and treatment outcomes were analyzed. RESULTS: Five patients (ten eyes) with bilateral simultaneous infective keratitis were identified...
March 27, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/28330899/il-24-promotes-pseudomonas-aeruginosa-keratitis-in-c57bl-6-mouse-corneas
#8
Bing X Ross, Nan Gao, Xinhan Cui, Theodore J Standiford, Jianjiang Xu, Fu-Shin X Yu
The aim of this study was to elucidate the expression and functions of IL-24 in C57BL/6 mouse corneas in response to Pseudomonas aeruginosa infection. Among IL-20R cytokines, only IL-24 was induced at both mRNA and protein levels by infection at early time points. The upregulation of IL-24 was dampened by flagellin pretreatment, which protects the corneas from microbial infection. Time course studies revealed bimodal early and later peaks of IL-24 expression, a pattern shared with suppressor of cytokine signaling (SOCS)3 but not IL-1β or IL-6...
May 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28298316/paediatric-infectious-keratitis-a-case-series-of-107-children-presenting-to-a-tertiary-referral-centre
#9
Julia Dutra Rossetto, Kara M Cavuoto, Carla J Osigian, Ta Chen Peter Chang, Darlene Miller, Hilda Capo, Oriel Spierer
BACKGROUND/AIMS: Corneal ulcers can result in severe visual impairment in children. The recent trends of paediatric microbial ulcerative keratitis in the USA are unknown. The purpose of this study is to report the risk factors, microbiological profile and treatment outcomes of paediatric microbial keratitis in South Florida. METHODS: A university-based tertiary eye care centre retrospective case series between 1992 and 2015. Medical records of 107 paediatric patients (age <18 years) with the diagnosis of microbial ulcerative keratitis were analysed...
March 15, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28295043/chlorin-e6-mediated-photodynamic-inactivation-for-multidrug-resistant-pseudomonas-aeruginosa-keratitis-in-mice-in-vivo
#10
Ming-Feng Wu, Mona Deichelbohrer, Thomas Tschernig, Matthias W Laschke, Nóra Szentmáry, Dirk Hüttenberger, Hans-Jochen Foth, Berthold Seitz, Markus Bischoff
Following corneal epithelium scratches, mouse corneas were infected with the multidrug resistant (MDR) P. aeruginosa strain PA54. 24 hours later, 0% (for control group), 0.01%, 0.05% or 0.1% Chlorin e6 (Ce6), a second generation photosensitizer derived from chlorophyll, was combined with red light, for photodynamic inactivation (PDI). 1 hour or 2 days later, entire mouse eyes were enucleated and homogenized for counting colony forming units (CFU) of P. aeruginosa. For comparison, 0.1% Ce6 mediated PDI was started at 12 hours post infection, and 0...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28292273/ocular-bacterial-infections-at-quiha-ophthalmic-hospital-northern-ethiopia-an-evaluation-according-to-the-risk-factors-and-the-antimicrobial-susceptibility-of-bacterial-isolates
#11
Mebrahtu Teweldemedhin, Muthupandian Saravanan, Araya Gebreyesus, Dawit Gebreegziabiher
BACKGROUND: External and intraocular infections can lead to visual impairments, which is a major public health problem. Bacteria are the most frequent pathogens affecting ocular structures; the increasing rate of antimicrobial drug resistance is a worldwide concern. The aim of this study was to determine the occurrence of bacteria in ocular infections, their antimicrobial susceptibility patterns, and risk factors in bacterial ocular infection. METHODS: A hospital based cross-sectional study was conducted from September 2015 to December 2015 at Quiha Ophthalmic Hospital, Tigray, northern Ethiopia...
March 14, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28261183/effect-of-quorum-quenching-lactonase-in-clinical-isolates-of-pseudomonas-aeruginosa-and-comparison-with-quorum-sensing-inhibitors
#12
Assia Guendouze, Laure Plener, Janek Bzdrenga, Pauline Jacquet, Benjamin Rémy, Mikael Elias, Jean-Philippe Lavigne, David Daudé, Eric Chabrière
Pseudomonas aeruginosa is a Gram negative pathogenic bacterium involved in many human infections including otitis, keratitis, pneumonia, and diabetic foot ulcers. P. aeruginosa uses a communication system, referred to as quorum sensing (QS), to adopt a group behavior by synchronizing the expression of certain genes. Among the regulated traits, secretion of proteases or siderophores, motility and biofilm formation are mainly involved in the pathogenicity. Many efforts have been dedicated to the development of quorum sensing inhibitors (QSI) and quorum quenching (QQ) agents to disrupt QS...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28236499/clinical-features-and-microbiological-in-bacterial-keratitis-in-a-tertiary-referral-hospital
#13
J M Ruiz Caro, L Cabrejas, M R de Hoz, D Mingo, S P Duran
OBJECTIVE: To describe the clinical features, bacterial agents, and antibiotic sensitivity of bacterial keratitis in the Ophthalmology Department at the University Hospital Fundación Jiménez Díaz (HUFJD) in Madrid. MATERIALS AND METHODS: A retrospective observational descriptive study using clinical records and reports of corneal scrapings in patients with bacterial keratitis at the HUFJD conducted between 2009 and 2014. RESULTS: In a sample of 160 patients, gram-positive bacteria were the most prevalent with 64...
February 21, 2017: Archivos de la Sociedad Española de Oftalmología
https://www.readbyqxmd.com/read/28217443/virulence-genome-analysis-of-pseudomonas-aeruginosa-vrfpa10-recovered-from-patient-with-scleritis
#14
Nandagopal Murugan, Jambulingam Malathi, Vetrivel Umashankar, Hajib Narahari Rao Madhavan
Infectious keratitis is a major cause of blindness, next to cataract and majority of cases are mainly caused by gram negative bacterium Pseudomonas aeruginosa (P. aeruginosa). In this study, we investigated a P. aeruginosa VRFPA10 genome which exhibited susceptibility to commonly used drugs in vitro but the patient had poor prognosis due to its hyper virulent nature. Genomic analysis of VRFPA10 deciphered multiple virulence factors and P.aeruginosa Genomic Islands (PAGIs) VRFPA10 genome which correlated with hyper virulence nature of the organism...
June 2017: Genomics Data
https://www.readbyqxmd.com/read/28151861/shifting-trends-in-microbial-keratitis-following-penetrating-keratoplasty-in-taiwan
#15
Hung-Chi Chen, Chia-Yi Lee, Hung-Yu Lin, David Hui-Kang Ma, Phil Yeong-Fong Chen, Ching-Hsi Hsiao, Hsin-Chiung Lin, Lung-Kun Yeh, Hsin-Yuan Tan
To investigate the clinical and microbiological profiles from microbial keratitis following penetrating keratoplasty (PKP) in a tertiary referral center in Taiwan, the medical records of 648 consecutive patients (648 eyes) undergoing PKP between January 2003 and December 2007 were retrospectively reviewed. Patients who subsequently sustained microbial keratitis were enrolled and analyzed for potential risk factors, clinical manifestations, microbiological profiles, complications, graft survival, and final visual outcome...
February 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28149778/epidemiological-clinical-and-laboratory-findings-of-infectious-keratitis-at-mansoura-ophthalmic-center-egypt
#16
Amani E Badawi, Dalia Moemen, Nora L El-Tantawy
AIM: To analyze the epidemiological, clinical and laboratory findings of infectious keratitis. METHODS: A retrospective study on cases of infective keratitis, attended our institution from Mar. 2013 to Feb. 2015, was done at Mansoura Ophthalmic Center, Egypt. Corneal scrapings were performed and processed for direct microscopy and culture in appropriate media using standard laboratory protocols. RESULTS: Out of 245 patients enrolled for study, 247 corneal scrapings were obtained...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28129422/antimicrobial-blue-light-therapy-for-infectious-keratitis-ex-vivo-and-in-vivo-studies
#17
Hong Zhu, Irene E Kochevar, Irmgard Behlau, Jie Zhao, Fenghua Wang, Yucheng Wang, Xiaodong Sun, Michael R Hamblin, Tianhong Dai
Purpose: To investigate the effectiveness of antimicrobial blue light (aBL) as an alternative or adjunctive therapeutic for infectious keratitis. Methods: We developed an ex vivo rabbit model and an in vivo mouse model of infectious keratitis. A bioluminescent strain of Pseudomonas aeruginosa was used as the causative pathogen, allowing noninvasive monitoring of the extent of infection in real time via bioluminescence imaging. Quantitation of bacterial luminescence was correlated to colony-forming units (CFU)...
January 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28123711/gaseous-nitric-oxide-for-the-local-treatment-of-bacterial-keratitis-in-mice
#18
Mona Deichelbohrer, Ming-Feng Wu, Berthold Seitz, Stefan Wagenpfeil, Carola Meier, Markus Bischoff, Thomas Tschernig
The successful treatment of severe bacterial keratitis continues to be a challenge in animals and humans. In the present study the aim was to assess gaseous therapy using gaseous nitric oxide (gNO) in a murine model of Pseudomonas aeruginosa keratitis. The cornea of anesthetized mice was mechanically scratched and covered with a bacterial suspension of P. aeruginosa. One day later, the infected eyes were exposed to 200 ppm NO for 30 min. Three to seven days later the mice were sacrificed and the bulbi were obtained and processed for light microscopy...
January 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28092397/update-on-orthokeratology-in-managing-progressive-myopia-in-children-efficacy-mechanisms-and-concerns
#19
Xintong Li, Ilana B Friedman, Norman B Medow, Cheng Zhang
Myopia is an important public health issue, and high myopia may lead to severe complications if left untreated. Orthokeratology lenses, worn overnight to reshape the cornea, are one of many recent modalities used to slow down the progression of myopia in children. This treatment has been proven successful, as evidenced by decreased spherical refractive error and axial length relative to the control at interval follow-up ranging from 6 months to 5 years. In this systematic review, the authors collected published controlled studies that analyzed the efficacy of orthokeratology lens wear and calculated longitudinal relative changes in axial length, revealing a weighted average of -45...
May 1, 2017: Journal of Pediatric Ophthalmology and Strabismus
https://www.readbyqxmd.com/read/28079686/number-of-bacteria-and-time-of-coincubation-with-bacteria-required-for-the-development-of-acanthamoeba-keratitis
#20
Hayate Nakagawa, Takaaki Hattori, Naohito Koike, Tomoko Ehara, Akitomo Narimatsu, Shigeto Kumakura, Tetsuya Matsumoto, Hiroshi Goto
PURPOSE: We hypothesized that bacteria may be a factor contributing to the development of Acanthamoeba keratitis (AK). We investigated interactions between Acanthamoeba and Pseudomonas aeruginosa for the development of keratitis in rabbit corneas. METHODS: Acanthamoeba castellanii (ATCC50492) and P. aeruginosa (PAO-1) were used. Two densities of P. aeruginosa (high, 1 × 10/mL; low, 3 × 10/mL) and 2 durations of coincubation (long, 6 h; short, 2 h) of Acanthamoeba with 1 × 10/mL of P...
March 2017: Cornea
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