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pseudomonas keratitis

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https://www.readbyqxmd.com/read/29436623/development-of-uracil-dna-glycosylase-supplemented-loop-mediated-isothermal-amplification-coupled-with-nanogold-probe-udg-lamp-aunp-for-specific-detection-of-pseudomonas%C3%A2-aeruginosa
#1
Orapan Manajit, Siwaporn Longyant, Paisarn Sithigorngul, Parin Chaivisuthangkura
Pseudomonas aeruginosa (P. aeruginosa) is an important opportunistic pathogen that causes serious infections in humans, including keratitis in contact lens wearers. Therefore, establishing a rapid, specific and sensitive method for the identification of P. aeruginosa is imperative. In the present study, the uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification combined with nanogold labeled hybridization probe (UDG-LAMP-AuNP) was developed for the detection of P. aeruginosa. UDG-LAMP was performed to prevent carry over contamination and the LAMP reactions can be readily observed using the nanogold probe...
February 2, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29369234/in-vitro-evaluation-of-a-hypochlorous-acid-hygiene-solution-on-established-biofilms
#2
Eric G Romanowski, Nicholas A Stella, Kathleen A Yates, Kimberly M Brothers, Regis P Kowalski, Robert M Q Shanks
OBJECTIVES: The purpose of this study was to determine whether a commercial formulation of hypochlorous acid hygiene solution (0.01%), Avenova, can destroy existing biofilms formed by ocular clinical bacterial isolates, including blepharitis isolates of Staphylococcus aureus and coagulase-negative staphylococci, and a keratitis isolate of Pseudomonas aeruginosa. METHODS: Biofilms grown in bacterial growth media on disposable contact lens cases were challenged with hypochlorous acid hygiene solution...
January 15, 2018: Eye & Contact Lens
https://www.readbyqxmd.com/read/29346493/antimicrobial-studies-using-the-therapeutic-tissue-cross-linking-agent-sodium-hydroxymethylglycinate-implication-for-treating-infectious-keratitis
#3
Patrick B Rapuano, Alexandra H Scanameo, Daeryl E Amponin, Sefy A Paulose, Mariya Zyablitskaya, Anna Takaoka, Leejee H Suh, Takayuki Nagasaki, Stephen L Trokel, David C Paik
Purpose: Our recent studies raise the possibility of using sodium hydroxymethylglycinate (SMG), for pharmacologic therapeutic tissue cross-linking (TXL) of the cornea. The present study was performed to evaluate the antimicrobial effects of SMG for potential use in treating infectious keratitis. Methods: In initial (group 1) experiments, methicillin-sensitive Staphylococcus aureus (MSSA), methicillin-resistant Staphylococcus aureus (MRSA), and Pseudomonas aeruginosa (PA) were treated with SMG (10-40 mM) for 10 to 120 minutes...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29341837/ocular-permeation-of-topical-tazocin-and-its-effectiveness-in-the-treatment-of-pseudomonas-aeruginosa-induced-keratitis-in-rabbits
#4
Sujaya Singh, Renu Agarwal, Zulhilmi Abdul Razak, Robin Ngu, Lwin L Nyein, Sushil Vasudevan, Marina Kapitonova, Azida Juana Kadir, Subrayan Visvaraja
PURPOSE: Pseudomonas aeruginosa is the most common causative organism for contact lens-associated corneal ulcer and is commonly treated with fluoroquinolones. With the emergence of resistant strains, it is important to investigate alternative therapies. Despite well-established efficacy of tazocin against systemic Pseudomonas infections, its topical use for the treatment of Pseudomonas keratitis has not been described, hence this study was aimed to find the ocular permeation of Tazocin and its efficacy in treating keratitis in rabbit eyes...
January 17, 2018: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/29335265/contact-lens-related-pseudomonas-aeruginosa-keratitis-in-a-49-year-old-woman
#5
Harald Gjerde, Anuradha Mishra
No abstract text is available yet for this article.
January 15, 2018: CMAJ: Canadian Medical Association Journal, Journal de L'Association Medicale Canadienne
https://www.readbyqxmd.com/read/29325116/differential-expression-of-antimicrobial-peptides-in-corneal-infection-and-regulation-of-antimicrobial-peptides-and-reactive-oxygen-species-by-type-iii-secretion-system-of-pseudomonas-aeruginosa
#6
Prerana Sharma, Sanjukta Guha, Prashant Garg, Sanhita Roy
Pseudomonas aeruginosa is an opportunistic pathogen and is the major cause of corneal infection worldwide that secret several virulent toxins through its type III secretion system (T3SS). In defense against pathogenic insults, epithelial cells and macrophages express antimicrobial peptides (AMPs) that are essential components of host immune response. In this study, we have determined the expression of several AMPs in patients with P. aeruginosa corneal infection. We also used an in vitro model of infection using human corneal epithelial cells and macrophages to determine the gene expression of AMPs and cellular response to wild-type and T3SS mutant P...
January 9, 2018: Pathogens and Disease
https://www.readbyqxmd.com/read/29278804/adherence-of-pseudomonas-aeruginosa-onto-surfactant-laden-contact-lenses
#7
Reynalyn Mosuela, Shelan Mustafa, Simon Gould, Hany Hassanin, Raid G Alany, Amr ElShaer
There is an immense research interest to utilise contact lens (CLs) as a popular platform for ocular drug delivery. However, CLs are the major predisposing factors of bacterial keratitis which is commonly caused by adhesion of microbes such as Pseudomonas aeruginosa and Staphylococcus epidermidis. The aim of the current study is to explore the effect of surfactants; Poloxamer 188, Polysorbate 80 and Tetronic® 90R4 (at 0.25% - 3% v/v) on the characteristics of CLs and on the adhesion abilities of Pseudomonas aeruginosa to the lenses' surfaces...
December 13, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29273244/bacterial-keratitis-treated-by-strengthened-antibiotic-eye-drops-an-18-months-review-of-clinical-cases-and-antibiotic-susceptibilities
#8
J Saillard, L Spiesser-Robelet, P Gohier, T Briot
PURPOSE: To describe, in patients treated for infectious keratitis, the microorganisms identified and their antibiotic susceptibility over a period of 18 months. METHOD: Retrospective, descriptive, non-comparative study. Medical and biological data were extracted from the patients' file treated with strengthened antibiotic eye drops at Angers University Hospital between January 2015 and June 2016. The main elements noted were the bacteria involved and their susceptibility to antibiotics...
December 19, 2017: Annales Pharmaceutiques Françaises
https://www.readbyqxmd.com/read/29242901/designed-host-defense-peptides-for-the-treatment-of-bacterial-keratitis
#9
L Edward Clemens, Jesse Jaynes, Edward Lim, Satya S Kolar, Rose Y Reins, Hasna Baidouri, Samuel Hanlon, Alison M McDermott, Kathryn W Woodburn
Purpose: To limit corneal damage and potential loss of vision, bacterial keratitis must be treated aggressively. Innovation in antimicrobials is required due to the need for empirical treatment and the rapid emergence of bacterial resistance. Designed host defense peptides (dHDPs) are synthetic analogues of naturally occurring HDPs, which provide defense against invading pathogens. This study investigates the use of novel dHDPs for the treatment of bacterial keratitis. Methods: The minimum inhibitory concentrations (MICs) were determined for dHDPs on both Gram-positive and -negative bacteria...
December 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29236588/topical-glycyrrhizin-is-therapeutic-for-pseudomonas-aeruginosa-keratitis
#10
Sandamali A Ekanayaka, Sharon A McClellan, Ronald P Barrett, Linda D Hazlett
PURPOSE: Glycyrrhizin (GLY), an inhibitor of high-mobility group box 1 (HMGB1) protects prophylactically against Pseudomonas aeruginosa keratitis. However, the therapeutic potential of GLY to enhance an antibiotic has not been tested and is our purpose. METHODS: C57BL/6 mice (B6) were infected with a clinical isolate (KEI 1025) of P. aeruginosa and treated topically at 6 h postinfection (p.i.) with GLY or phosphate-buffered saline (PBS). Clinical scores, photography with a slit lamp, enzyme-linked immunosorbent assay, myeloperoxidase assay, bacterial plate counts, histopathology, reactive oxygen/nitrogen species (ROS/RNS) assays, and in vitro macrophage (Mφ) stimulation assays were used to assess effects of GLY treatment...
December 13, 2017: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/29178851/bacterial-profile-of-ocular-infections-a-systematic-review
#11
REVIEW
Mebrahtu Teweldemedhin, Hailay Gebreyesus, Ataklti Hailu Atsbaha, Solomon Weldegebreal Asgedom, Muthupandian Saravanan
BACKGROUND: Bacteria are the major contributor of ocular infections worldwide. Ocular infections, if left untreated, can damage the structures of the eye with possible blindness and visual impairments. This work was aimed to review the bacterial profile of ocular infections. METHODS: Literature search was made in different electronic databases; the review was systematically made to get concrete findings. RESULTS: As far as this review, Staphylococcus aureus, Coagulase negative Staphylococci, Streptococcus pneumoniae and Pseudomonas aeruginosa are the leading isolates in ocular infections...
November 25, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/29104658/bacterial-keratitis-photodynamic-inactivation-reduced-experimental-inflammation
#12
Mona Deichelbohrer, Ming-Feng Wu, Berthold Seitz, Dirk Hüttenberger, Matthias W Laschke, Hans-Jochen Foth, Bodo Wonnenberg, Stefan Wagenpfeil, Carola Meier, Markus Bischoff, Thomas Tschernig
The successful treatment of bacterial keratitis remains an unsolved clinical problem. The current study aimed to establish a murine keratitis model and to investigate the effect of chlorin e6 (Ce6) and photodynamic inactivation (PDI) on corneal inflammation. The cornea of anesthetized mice was scratched and covered with a bacterial suspension of Pseudomonas aeruginosa. A paste containing Ce6 was applied to the cornea with subsequent exposure to specified light. Two days later the animals were sacrificed, and the globes were processed for light microscopy...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29064403/characterization-of-three-ocular-clinical-isolates-of-p-aeruginosa-viability-biofilm-formation-adherence-infectivity-and-effects-of-glycyrrhizin
#13
Xudong Peng, Sandamali A Ekanayaka, Sharon A McClellan, Ronald P Barrett, Kerry Vistisen, Linda D Hazlett
We selectively characterized three isolates from Pseudomonas aeruginosa keratitis patients and how glycyrrhizin (GLY) affected them. Type III toxins were determined using polymerase chain reaction (PCR). Minimum Inhibitory Concentration (MIC) of GLY and assays for its effects on: time kill, bacterial permeability, and biofilm/adhesion were done. In vivo, C57BL/6 (B6) mice were treated topically with GLY after G81007 infection. Clinical score, photography with a slit lamp and RT-PCR were used to assess treatment effects...
October 24, 2017: Pathogens
https://www.readbyqxmd.com/read/29053557/bacterial-keratitis-isolated-organisms-and-antibiotic-resistance-patterns-in-san-francisco
#14
Michelle Y Peng, Vicky Cevallos, Stephen D McLeod, Thomas M Lietman, Jennifer Rose-Nussbaumer
PURPOSE: Understanding the spectrum of pathogens in a given geographic region is important when deciding on empiric antibiotic therapy. In this study, we evaluate the spectrum of bacterial organisms cultured from corneal samples and their antibiotic sensitivities to guide initial treatment of keratitis. METHODS: We performed a retrospective case review of cultures from suspected infectious keratitis cases at the Francis I. Proctor Foundation, University of California, San Francisco, from 1996 through 2015...
October 17, 2017: Cornea
https://www.readbyqxmd.com/read/29044738/overview-of-mechanisms-of-antibiotic-resistance-in-pseudomonas-aeruginosa-an-ocular-perspective
#15
REVIEW
Dinesh Subedi, Ajay Kumar Vijay, Mark Willcox
Treatment of Pseudomonas aeruginosa eye infections often becomes a challenge due to the ability of this bacterium to be resistant to antibiotics via intrinsic and acquired mechanisms. Transfer of resistance due to interchangeable genetic elements is an important mechanism for the rapid transfer of antibiotic resistance in this pathogen. As a result, drug-resistant strains are becoming increasingly prevalent worldwide. This review systematically analyses data from recent publications to describe the global prevalence and antibiotic sensitivity of ocular P...
October 18, 2017: Clinical & Experimental Optometry: Journal of the Australian Optometrical Association
https://www.readbyqxmd.com/read/28993066/clinical-and-microbiological-profile-of-infectious-keratitis-in-an-area-of-madrid-spain
#16
Soraya Mediero, Ana Boto de Los Bueis, Karina Spiess, Mariana Díaz-Almirón, Almudena Del Hierro Zarzuelo, Isabel Villalaín Rodes, Adela García Perea
INTRODUCTION: To study antibiotic susceptibility in bacterial keratitis (BK), its profile over 10 years and its influence on ophthalmological practice. METHODS: Retrospective review of BK with positive corneal scraping over a 10-year period. Risk factors for keratitis, visual acuity (VA), empirical topical treatment, corneal infection characteristics and outcomes were analyzed for BK due to Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, Pseudomonas aeruginosa and Propionibacterium acnes...
October 6, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28980569/-severe-bilateral-pseudomonas-sclerokeratitis-in-comatose-patient-clinical-case
#17
Evg A Kasparova, A A Kasparov, E A Kasparova, A V Zaytsev
The article presents a clinical case of severe bilateral pseudomonas sclerokeratitis in a patient with occlusion hydrocephalus and intracranial hypertension, who was in a coma and on a ventilator for 20 days. At first examination (7 days after the onset of purulent keratitis, during which the process had been rapidly progressing), the clinical picture included lagophthalmos, severe purulent corneal ulcer, bilateral purulent scleromalacia, perforated cornea in the left eye. On the same day, in order to maintain eye integrity, urgent reconstructive penetrating sclerokeratoplasty with subsequent sclerocorneal coating was performed in both eyes right in the intensive care unit...
2017: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/28961278/nlrc4-regulates-caspase-1-and-il-1beta-production-in-a-cd11blowly6glow-population-of-cells-required-for-resistance-to-pseudomonas-aeruginosa-keratitis
#18
Sharon A McClellan, Andrew Jerome, Susmit Suvas, Linda D Hazlett
Psbetaeudomonas (P.) aeruginosa infection of the cornea in BALB/c mice does not result in perforation and the mice have been classified as resistant. However, regulation of this response via inflammasome activation remained untested. Therefore, BALB/c mice were infected with P. aeruginosa ATCC strain 19660 and NLRP3 and NLRC4 protein tested by ELISA. Since NLRC4 vs NLRP3 protein levels were significantly higher in the corneas of BALB/c at 1 and 5 days postinfection we used silencing to knockdown NLRC4. Silencing NLRC4 vs scrambled siRNA treatment exacerbated disease in BALB/c mice, reduced myeloperoxidase levels and elevated bacterial plate counts at 5 days postinfection...
2017: PloS One
https://www.readbyqxmd.com/read/28942073/update-on-the-management-of-infectious-keratitis
#19
REVIEW
Ariana Austin, Tom Lietman, Jennifer Rose-Nussbaumer
Infectious keratitis is a major global cause of visual impairment and blindness, often affecting marginalized populations. Proper diagnosis of the causative organism is critical, and although culture remains the prevailing diagnostic tool, newer techniques such as in vivo confocal microscopy are helpful for diagnosing fungus and Acanthamoeba. Next-generation sequencing holds the potential for early and accurate diagnosis even for organisms that are difficult to culture by conventional methods. Topical antibiotics remain the best treatment for bacterial keratitis, and a recent review found all commonly prescribed topical antibiotics to be equally effective...
November 2017: Ophthalmology
https://www.readbyqxmd.com/read/28938380/bacterial-keratitis-in-toronto-a-16-year-review-of-the-microorganisms-isolated-and-the-resistance-patterns-observed
#20
Alex L C Tam, Elie Côté, Mario Saldanha, Alejandro Lichtinger, Allan R Slomovic
PURPOSE: To review the incidence, distribution, current trends, and resistance patterns of bacterial keratitis isolates in Toronto over the past 16 years. METHODS: Microbiology records of suspected bacterial keratitis that underwent a diagnostic corneal scraping and cultures from January 1, 2000, through December 31, 2015, were retrospectively reviewed. The distribution of the main isolated pathogens and in vitro laboratory minimum inhibitory concentration testing results were used to identify resistance patterns...
December 2017: Cornea
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