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pseudomonas keratitis

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https://www.readbyqxmd.com/read/28217443/virulence-genome-analysis-of-pseudomonas-aeruginosa-vrfpa10-recovered-from-patient-with-scleritis
#1
Nandagopal Murugan, Jambulingam Malathi, Vetrivel Umashankar, Hajib Narahari Rao Madhavan
Infectious keratitis is a major cause of blindness, next to cataract and majority of cases are mainly caused by gram negative bacterium Pseudomonas aeruginosa (P. aeruginosa). In this study, we investigated a P. aeruginosa VRFPA10 genome which exhibited susceptibility to commonly used drugs in vitro but the patient had poor prognosis due to its hyper virulent nature. Genomic analysis of VRFPA10 deciphered multiple virulence factors and P.aeruginosa Genomic Islands (PAGIs) VRFPA10 genome which correlated with hyper virulence nature of the organism...
June 2017: Genomics Data
https://www.readbyqxmd.com/read/28151861/shifting-trends-in-microbial-keratitis-following-penetrating-keratoplasty-in-taiwan
#2
Hung-Chi Chen, Chia-Yi Lee, Hung-Yu Lin, David Hui-Kang Ma, Phil Yeong-Fong Chen, Ching-Hsi Hsiao, Hsin-Chiung Lin, Lung-Kun Yeh, Hsin-Yuan Tan
To investigate the clinical and microbiological profiles from microbial keratitis following penetrating keratoplasty (PKP) in a tertiary referral center in Taiwan, the medical records of 648 consecutive patients (648 eyes) undergoing PKP between January 2003 and December 2007 were retrospectively reviewed. Patients who subsequently sustained microbial keratitis were enrolled and analyzed for potential risk factors, clinical manifestations, microbiological profiles, complications, graft survival, and final visual outcome...
February 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28149778/epidemiological-clinical-and-laboratory-findings-of-infectious-keratitis-at-mansoura-ophthalmic-center-egypt
#3
Amani E Badawi, Dalia Moemen, Nora L El-Tantawy
AIM: To analyze the epidemiological, clinical and laboratory findings of infectious keratitis. METHODS: A retrospective study on cases of infective keratitis, attended our institution from Mar. 2013 to Feb. 2015, was done at Mansoura Ophthalmic Center, Egypt. Corneal scrapings were performed and processed for direct microscopy and culture in appropriate media using standard laboratory protocols. RESULTS: Out of 245 patients enrolled for study, 247 corneal scrapings were obtained...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28129422/antimicrobial-blue-light-therapy-for-infectious-keratitis-ex-vivo-and-in-vivo-studies
#4
Hong Zhu, Irene E Kochevar, Irmgard Behlau, Jie Zhao, Fenghua Wang, Yucheng Wang, Xiaodong Sun, Michael R Hamblin, Tianhong Dai
Purpose: To investigate the effectiveness of antimicrobial blue light (aBL) as an alternative or adjunctive therapeutic for infectious keratitis. Methods: We developed an ex vivo rabbit model and an in vivo mouse model of infectious keratitis. A bioluminescent strain of Pseudomonas aeruginosa was used as the causative pathogen, allowing noninvasive monitoring of the extent of infection in real time via bioluminescence imaging. Quantitation of bacterial luminescence was correlated to colony-forming units (CFU)...
January 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28123711/gaseous-nitric-oxide-for-the-local-treatment-of-bacterial-keratitis-in-mice
#5
Mona Deichelbohrer, Ming-Feng Wu, Berthold Seitz, Stefan Wagenpfeil, Carola Meier, Markus Bischoff, Thomas Tschernig
The successful treatment of severe bacterial keratitis continues to be a challenge in animals and humans. In the present study the aim was to assess gaseous therapy using gaseous nitric oxide (gNO) in a murine model of Pseudomonas aeruginosa keratitis. The cornea of anesthetized mice was mechanically scratched and covered with a bacterial suspension of P. aeruginosa. One day later, the infected eyes were exposed to 200 ppm NO for 30 min. Three to seven days later the mice were sacrificed and the bulbi were obtained and processed for light microscopy...
January 2017: Biomedical Reports
https://www.readbyqxmd.com/read/28092397/update-on-orthokeratology-in-managing-progressive-myopia-in-children-efficacy-mechanisms-and-concerns
#6
Xintong Li, Ilana B Friedman, Norman B Medow, Cheng Zhang
Myopia is an important public health issue, and high myopia may lead to severe complications if left untreated. Orthokeratology lenses, worn overnight to reshape the cornea, are one of many recent modalities used to slow down the progression of myopia in children. This treatment has been proven successful, as evidenced by decreased spherical refractive error and axial length relative to the control at interval follow-up ranging from 6 months to 5 years. In this systematic review, the authors collected published controlled studies that analyzed the efficacy of orthokeratology lens wear and calculated longitudinal relative changes in axial length, revealing a weighted average of -45...
January 13, 2017: Journal of Pediatric Ophthalmology and Strabismus
https://www.readbyqxmd.com/read/28079686/number-of-bacteria-and-time-of-coincubation-with-bacteria-required-for-the-development-of-acanthamoeba-keratitis
#7
Hayate Nakagawa, Takaaki Hattori, Naohito Koike, Tomoko Ehara, Akitomo Narimatsu, Shigeto Kumakura, Tetsuya Matsumoto, Hiroshi Goto
PURPOSE: We hypothesized that bacteria may be a factor contributing to the development of Acanthamoeba keratitis (AK). We investigated interactions between Acanthamoeba and Pseudomonas aeruginosa for the development of keratitis in rabbit corneas. METHODS: Acanthamoeba castellanii (ATCC50492) and P. aeruginosa (PAO-1) were used. Two densities of P. aeruginosa (high, 1 × 10/mL; low, 3 × 10/mL) and 2 durations of coincubation (long, 6 h; short, 2 h) of Acanthamoeba with 1 × 10/mL of P...
March 2017: Cornea
https://www.readbyqxmd.com/read/28079684/the-cif-virulence-factor-gene-is-present-in-isolates-from-patients-with-pseudomonas-aeruginosa-keratitis
#8
Christopher D Bahl, Jessica D St Laurent, R Siva Ganesa Karthikeyan, J Lakshmi Priya, Lalitha Prajna, Michael E Zegans, Dean R Madden
PURPOSE: To determine whether the cif gene is present in pathogenic Pseudomonas aeruginosa isolates from patients with bacterial keratitis at Aravind Eye Hospital, a referral eye care center in southern India, and from corresponding environmental isolates. METHODS: Polymerase chain reaction amplification was performed on strains of P. aeruginosa isolated from ocular infections and environmental soil samples were collected from the area surrounding Aravind Eye Hospital...
March 2017: Cornea
https://www.readbyqxmd.com/read/28002114/treatment-of-pseudomonas-keratitis-by-continuous-infusion-of-topical-antibiotics-with-the-morgan-lens
#9
Mingwu Wang, Whitney A Smith, Joshua K Duncan, Joseph M Miller
PURPOSE: Despite following standard treatment, Pseudomonas keratitis can continue to progress and result in loss of vision or of the eye. Our cases demonstrate that the Morgan Lens can be an effective topical antibiotic delivery vehicle in advanced keratitis. METHODS: Two patients (3 eyes) with Pseudomonas keratitis were included in this report after failing to respond to intense inpatient topical treatment. Because loss of the eyes was imminent, the Morgan Lenses were used for continuous lavage with ceftazidime (50 mg/mL), in conjunction with other conventional treatment...
December 20, 2016: Cornea
https://www.readbyqxmd.com/read/27998732/pathogenesis-of-microbial-keratitis
#10
REVIEW
Sahreena Lakhundi, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Microbial keratitis is a sight-threatening ocular infection caused by bacteria, fungi, and protist pathogens. Epithelial defects and injuries are key predisposing factors making the eye susceptible to corneal pathogens. Among bacterial pathogens, the most common agents responsible for keratitis include Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pneumonia and Serratia species. Fungal agents of corneal infections include both filamentous as well as yeast, including Fusarium, Aspergillus, Phaeohyphomycetes, Curvularia, Paecilomyces, Scedosporium and Candida species, while in protists, Acanthamoeba spp...
December 18, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27995356/tripartite-motif-8-trim8-positively-regulates-pro-inflammatory-responses-in-pseudomonas-aeruginosa-induced-keratitis-through-promoting-k63-linked-polyubiquitination-of-tak1-protein
#11
Litao Guo, Weili Dong, Xiaoxiao Fu, Jing Lin, Zhijun Dong, Xiaobo Tan, Tiemin Zhang
Pseudomonas aeruginosa (PA)-induced keratitis is a rapidly progressive ocular infectious disease that often leads to inflammatory epithelial edema, stromal infiltration, tissue destruction, corneal ulceration, and vision loss. In this study, we investigate the role of tripartite motif 8 (TRIM8) in regulating the inflammatory process of PA-induced keratitis. The expression of TRIM8 was increased in mouse corneas and in vitro-cultured macrophages after PA infection. Knockdown of the expression of TRIM8 significantly inhibited the activation of NF-κB signaling and decreased the production of pro-inflammatory cytokines both in vivo and in vitro after infected with PA...
December 19, 2016: Inflammation
https://www.readbyqxmd.com/read/27960579/genus-distribution-of-bacteria-and-fungi-associated-with-keratitis-in-a-large-eye-center-located-in-southern-china
#12
Lixia Lin, Weizhong Lan, Bingsheng Lou, Hongmin Ke, Yuanzhe Yang, Xiaofeng Lin, Lingyi Liang
PURPOSE: To investigate the genus distribution of bacteria and fungi associated with keratitis in a large eye center located in Southern China and to compare the results with existing data from other areas in China. METHODS: All results of corneal microbiological examinations from 2009 to 2013 of patients who had been clinically diagnosed with bacterial or fungal keratitis were obtained chronologically and anonymously from the microbiology database at Zhongshan Ophthalmic Center...
December 14, 2016: Ophthalmic Epidemiology
https://www.readbyqxmd.com/read/27928519/antimicrobial-susceptibility-and-minimal-inhibitory-concentration-of-pseudomonas-aeruginosa-isolated-from-septic-ocular-surface-disease-in-different-animal-species
#13
L Leigue, F Montiani-Ferreira, B A Moore
The purpose of this study was to evaluate the antibiotic susceptibility profile of Pseudomonas aeruginosa isolated from different animal species with septic ocular surface disease. Sixteen strains of P. aeruginosa were isolated from different species of animals (dog, cat, horse, penguin and brown bear) with ocular surface diseases such as conjunctivitis, keratocojnuctivits sicca and ulcerative keratitis. These isolates were tested against 11 different antimicrobials agents using the Kirby-Bauer disk-diffusion method...
2016: Open veterinary journal
https://www.readbyqxmd.com/read/27844206/ex-vivo-rabbit-and-human-corneas-as-models-for-bacterial-and-fungal-keratitis
#14
Abigail Pinnock, Nagaveni Shivshetty, Sanhita Roy, Stephen Rimmer, Ian Douglas, Sheila MacNeil, Prashant Garg
PURPOSE: In the study of microbial keratitis, in vivo animal models often require a large number of animals, and in vitro monolayer cell culture does not maintain the three-dimensional structure of the tissues or cell-to-cell communication of in vivo models. Here, we propose reproducible ex vivo models of single- and dual-infection keratitis as an alternative to in vivo and in vitro models. METHODS: Excised rabbit and human corneoscleral rims maintained in organ culture were infected using 10(8) cells of Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans or Fusarium solani...
February 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/27825482/unraveling-genomic-and-phenotypic-nature-of-multidrug-resistant-mdr-pseudomonas-aeruginosa-vrfpa04-isolated-from-keratitis-patient
#15
Murugan N, Malathi J, Umashankar V, Madhavan H N
Multidrug-resistant (MDR) Pseudomonas aeruginosa VRFPA04, obtained from a keratitis patient was found to exhibit resistance to betalactam (Penicillins, cephalosporins, including carbapenems, except aztreonam), aminoglycosides, quinolone group of drugs and susceptible to colistin. The complete genome sequencing of the ocular isolate to measure and ascertain the degree of multidrug resistance in VRFPA04 strain resulted in 6,818,030bp (6.8Mb) genome sizes, which happen to be the third largest genome available in the Genbank to date...
December 2016: Microbiological Research
https://www.readbyqxmd.com/read/27800266/polymicrobial-infection-of-the-cornea-due-to-contact-lens-wear
#16
Selçuk Sızmaz, Sibel Bingöllü, Elif Erdem, Filiz Kibar, Soner Koltaş, Meltem Yağmur, Reha Ersöz
A 38-year-old male presented with pain and redness in his left eye. He had a history of wearing contact lenses. His ophthalmic examination revealed a large corneal ulcer with surrounding infiltrate. Cultures were isolated from the contact lenses, lens solutions, storage cases, and conjunctivae of both eyes and also corneal scrapings of the left eye. Fortified vancomycin and amikacin drops were started hourly. Culture results of conjunctivae of each eye and left cornea were positive for Pseudomonas aeruginosa; cultures from the contact lenses, lens solution and storage case of both eyes revealed Pseudomonas aeruginosa and Alcaligenes xylosoxidans...
April 2016: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/27792814/glycyrrhizin-reduces-hmgb1-and-bacterial-load-in-pseudomonas-aeruginosa-keratitis
#17
Sandamali A Ekanayaka, Sharon A McClellan, Ronald P Barrett, Shikhil Kharotia, Linda D Hazlett
Purpose: High mobility group box 1 (HMGB1) contributes to poor disease outcome in Pseudomonas aeruginosa keratitis. This study tests the prophylactic effect of treatment with HMGB1 inhibitors, glycyrrhizin (GLY) and its derivative, carbenoxolone (CBX), for Pseudomonas keratitis. Methods: We treated C57BL/6 (B6) mice subconjunctivally with GLY or CBX, infected with a noncytotoxic clinical isolate (KEI 1025) or a cytotoxic strain (ATCC 19660) of P. aeruginosa, and injected intraperitoneally with either agent...
October 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27768798/melimine-coated-antimicrobial-contact-lenses-reduce-microbial-keratitis-in-an-animal-model
#18
Debarun Dutta, Ajay K Vijay, Naresh Kumar, Mark D P Willcox
Purpose: To determine the ability of antimicrobial peptide melimine-coated contact lenses to reduce the incidence of microbial keratitis (MK) in a rabbit model of contact lens wear. Methods: In vitro antimicrobial activity of melimine-coated contact lenses was determined against Pseudomonas aeruginosa by viable count and a radiolabeled assay. The amount of lipopolysaccharide (LPS) associated with bacteria bound to melimine-coated and control lenses was determined...
October 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27738866/the-comparison-of-antimicrobial-effectiveness-of-contact-lens-solutions
#19
Ali Kal, Mustafa Ilker Toker, Serpil Kaya
PURPOSE: The aim of this study was to compare the effects of widely used multipurpose contact lens solutions against Staphylococcus aureus and Pseudomonas aeruginosa, in addition to cystic and trophozoite forms of Acanthamoeba castellanii and A. polyphaga, that cause microbial keratitis. METHODS: Three multipurpose solutions were tested: SOLO-care, ReNu, and Opti-Free Express. The test solutions were challenged with P. aeruginosa (ATCC 27853) and S. aureus (ATCC 2913) based on the ISO stand-alone and regiment test procedure for disinfecting products, A...
October 13, 2016: International Ophthalmology
https://www.readbyqxmd.com/read/27670247/human-tear-fluid-reduces-culturability-of-contact%C3%A2-lens-associated-pseudomonas-aeruginosa-biofilms-but-induces-expression-of-the-virulence-associated-type-iii-secretion-system
#20
Yvonne T Wu, Connie Tam, Lucia S Zhu, David J Evans, Suzanne M J Fleiszig
PURPOSE: The type III secretion system (T3SS) is a significant virulence determinant for Pseudomonas aeruginosa. Using a rodent model, we found that contact lens (CL)-related corneal infections were associated with lens surface biofilms. Here, we studied the impact of human tear fluid on CL-associated biofilm growth and T3SS expression. METHODS: P. aeruginosa biofilms were formed on contact lenses for up to 7 days with or without human tear fluid, then exposed to tear fluid for 5 or 24 h...
January 2017: Ocular Surface
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