Luiza Dos Santos Heringer, Julia Rios Carvalho, Julia Teixeira Oliveira, Bruna Texeira Silva, Domethila Mariano de Souza Aguiar Dos Santos, Anna Lecticia Martinez Martinez Toledo, Laura Maria Borges Savoldi, Debora Magalhães Portela, Suelen Adriani Marques, Paula Campello Costa Lopes, Ana Maria Blanco Martinez, Henrique Rocha Mendonça
OBJECTIVE: Malformations of the polymicrogyria spectrum can be mimicked in rodents through neonatal transcranial focal cortical freeze lesions. The animals presenting the malformations present both altered synaptic events and epileptiform activity in the vicinity of the microgyrus, but the comprehension of their contribution to increased predisposition or severity of seizures require further studies. METHODS: In order to investigate these issues, we induced both microgyria and schizencephaly in 57 mice and evaluated: their convulsive susceptibility and severity after pentyleneterazol (PTZ) treatment, the quantification of their symmetric and asymmetric synapses, the morphology of their dendritic arbors, and the content of modulators of synaptogenesis, such as SPARC, gephyrin and GAP-43 within the adjacent visual cortex...
September 2022: Epilepsia Open