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https://www.readbyqxmd.com/read/29224178/evaluation-of-the-anti-inflammatory-and-urotoxicity-ameliorative-effects-of-%C3%AE-humulene-containing-active-fraction-of-emilia-sonchifolia-l-dc
#1
Gilcy George Kallivalappil, Girija Kuttan
In folklore medicine, the plant Emilia sonchifolia, belonging to the family Asteraceae, is used for treating tumour and inflammation. In our previous studies, we have done a thorough phytochemical investigation of E. sonchifolia with a report on its potent antimetastatic activity. Further, we isolated and characterised its active fraction (AFES) containing the major compound γ-humulene with an evaluation of the antiangiogenic effect of AFES (5 mg/kg b.wt.). In the first part of the present study, AFES in different concentrations was used for the assessment of its possible anti-inflammatory effect employing three in vivo inflammatory models...
December 9, 2017: Inflammopharmacology
https://www.readbyqxmd.com/read/29224171/genetic-mapping-and-validation-of-the-loci-controlling-7s-%C3%AE-and-11s-a-type-storage-protein-subunits-in-soybean-glycine-max-l-merr
#2
Jeffrey D Boehm, Vi Nguyen, Rebecca M Tashiro, Dale Anderson, Chun Shi, Xiaoguang Wu, Lorna Woodrow, Kangfu Yu, Yuhai Cui, Zenglu Li
Four soybean storage protein subunit QTLs were mapped using bulked segregant analysis and an F2 population, which were validated with an F5 RIL population. The storage protein globulins β-conglycinin (7S subunit) and glycinin (11S subunits) can affect the quantity and quality of proteins found in soybean seeds and account for more than 70% of the total soybean protein. Manipulating the storage protein subunits to enhance soymeal nutrition and for desirable tofu manufacturing characteristics are two end-use quality goals in soybean breeding programs...
December 9, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29223907/analytical-indicators-to-characterize-particulate-organic-matter-pom-and-its-evolution-in-french-vertical-flow-constructed-wetlands-vfcws
#3
M Kania, M Gautier, Z Ni, E Bonjour, R Guégan, P Michel, P Jame, J Liu, R Gourdon
The design of French VFCWs leads to the formation of a sludge layer at the surface of the first filters due to the retention of suspended solids from the percolation of unsettled wastewater. This layer plays a major role in the system but still little is known on its characteristics and evolutions. In this study, suspended solids and sludge deposits sampled from two French VFCW plants were analyzed by different methods in the objective to assess the evolution of particulate organic matter (POM) along the treatment chain and within the sludge layer, and identify relevant analytical indicators of these phenomena...
December 7, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29223879/internalization-of-a-sunflower-mannose-binding-lectin-into-phytopathogenic-fungal-cells-induces-cytotoxicity
#4
Marianela Del Rio, Laura de la Canal, Marcela Pinedo, Mariana Regente
Lectins are carbohydrate-affinity proteins with the ability to recognize and reversibly bind specific glycoconjugates. We have previously isolated a bioactive sunflower mannose-binding lectin belonging to the jacalin-related family called Helja. Despite of the significant number of plant lectins described in the literature, only a small group exhibits antifungal activity and the mechanism by which they kill fungi is still not understood. The aim of this work was to explore Helja activity on plant pathogenic fungi, and provide insights into its mechanism of action...
December 6, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/29223850/genome-wide-analysis-of-carotenoid-cleavage-oxygenase-genes-and-their-responses-to-various-phytohormones-and-abiotic-stresses-in-apple-malus-domestica
#5
Hongfei Chen, Xiya Zuo, Hongxia Shao, Sheng Fan, Juanjuan Ma, Dong Zhang, Caiping Zhao, Xiangyan Yan, Xiaojie Liu, Mingyu Han
Carotenoid cleavage oxygenases (CCOs) are able to cleave carotenoids to produce apocarotenoids and their derivatives, which are important for plant growth and development. In this study, 21 apple CCO genes were identified and divided into six groups based on their phylogenetic relationships. We further characterized the apple CCO genes in terms of chromosomal distribution, structure and the presence of cis-elements in the promoter. We also predicted the cellular localization of the encoded proteins. An analysis of the synteny within the apple genome revealed that tandem, segmental, and whole-genome duplication events likely contributed to the expansion of the apple carotenoid oxygenase gene family...
December 5, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29223802/proteogenomic-insights-into-uranium-tolerance-of-a-chernobyl-s-microbacterium-bacterial-isolate
#6
Gallois Nicolas, Alpha-Bazin Béatrice, Ortet Philippe, Barakat Mohamed, Piette Laurie, Long Justine, Berthomieu Catherine, Armengaud Jean, Chapon Virginie
Microbacterium oleivorans A9 is a uranium-tolerant actinobacteria isolated from the trench T22 located near the Chernobyl nuclear power plant. This site is contaminated with different radionuclides including uranium. To observe the molecular changes at the proteome level occurring in this strain upon uranyl exposure and understand molecular mechanisms explaining its uranium tolerance, we established its draft genome and used this raw information to perform an in-depth proteogenomics study. High-throughput proteomics were performed on cells exposed or not to 10μM uranyl nitrate sampled at three previously identified phases of uranyl tolerance...
December 6, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29223623/gene-indexed-mutations-in-maize-zea-mays
#7
Xiaoduo Lu, Jisheng Liu, Wen Ren, Qun Yang, Zhenguang Chai, Rumei Chen, Lei Wang, Jun Zhao, Zhihong Lang, Haiyang Wang, Yunliu Fan, Jiuran Zhao, Chunyi Zhang
The availability of the B73 inbred reference genome sets the stage for high- throughput functional characterisation of maize genes on a whole-genome scale. Among the 39,324 protein-coding genes predicted, the vast majority has been untapped due to the lack of suitable high throughput reverse genetic resources. We have generated a gene-indexed maize mutant collection through ethyl methanesulfonate (EMS) mutagenesis and detected the mutations by combining exome capture and next-generation sequencing. A total of 1,086 mutated M1 plants were sequenced and 195,268 CG>TA-type point mutations, including stop gain/loss, missplice, start gain/loss, and various non-synonymous protein mutations as well as 4,610 InDel mutations, were identified...
December 6, 2017: Molecular Plant
https://www.readbyqxmd.com/read/29223538/curcumin-induces-osteoblast-differentiation-through-mild-endoplasmic-reticulum-stress-mediated-such-as-bmp2-on-osteoblast-cells
#8
Hyo-Eun Son, Eun-Jung Kim, Won-Gu Jang
AIMS: Curcumin (diferuloylmethane or [1E,6E]-1,7-bis[4-hydroxy-3-methoxyphenyl]-1,6heptadiene-3,5-dione) is a phenolic natural product derived from the rhizomes of the turmeric plant, Curcuma longa. It is reported to have various biological actions such as anti-oxidative, anti-inflammatory, and anti-cancer effects. However, the molecular mechanism of osteoblast differentiation by curcumin has not yet been reported. MAIN METHODS: The cytotoxicity of curcumin was identified using the 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) assay...
December 6, 2017: Life Sciences
https://www.readbyqxmd.com/read/29223391/effects-of-a-hydroethanolic-extract-of-boophone-disticha-bulb-on-anxiety-related-behaviour-in-naive-balb-c-mice
#9
William Pote, Shamiso Musarira, Donald Chuma, Louis L Gadaga, Ellen Mwandiringana, Dexter Tagwireyi
ETHNOPHARMACOLOGICAL RELEVANCE: Boophone disticha is one of the most important medicinal bulbs of Southern Africa. Previous in vitro studies have shown that it's crude ethanolic extracts and some alkaloidal phytoconstituents possesses high affinity for the serotonin transporter protein (SERT) and serotonin receptor 1a (5HT1a) which are both implicated in the pathogenesis and treatment of anxiety disorders. However, there are no in vivo studies that validates the anxiolytic actions of the plant...
December 6, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29223344/native-vascular-related-nac-transcription-factors-are-efficient-regulator-of-multiple-classes-of-secondary-wall-associated-genes-in-banana
#10
Sanjana Negi, Himanshu Tak, T R Ganapathi
Secondary-wall deposition in xylem vessel elements is regulated by vascular-related NAC transcription factors (VNDs). We show that three banana VNDs (MusaVND1, MusaVND2 and MusaVND3) directly regulate multiple secondary-wall associated genes by binding to their 5'-upstream regulatory region. Transgenic banana harboring either PMusaVND1:GUS, PMusaVND2:GUS or PMusaVND3:GUS showed specific GUS staining in lignified tissues. MusaVND1, MusaVND2 and MusaVND3 encodes transcriptional-activators as its C-terminal region drive expression of reporter genes in vivo in yeast...
December 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29223343/biotic-factors-that-induce-the-tomato-ve1-r-gene
#11
Christian Danve Castroverde, Xin Xu, Ross N Nazar, Jane Robb
In tomato, Verticillium resistance is determined by the Ve gene locus encoding two leucine-rich repeat-receptor-like proteins (Ve1, Ve2). The resistance function usually is attributed to Ve1 alone, with two known alleles: Ve1, encoding a resistance protein, and ve1, with a premature stop codon encoding a truncated product. We have examined further Ve-gene expression in resistant and susceptible near-isolines of Verticillium-infected Craigella tomatoes, using both quantitative RT-PCR and an alternative RFLP assay...
December 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29223337/an-erf-transcription-factor-from-tamarix-hispida-thcrf1-can-adjust-osmotic-potential-and-reactive-oxygen-species-scavenging-capability-to-improve-salt-tolerance
#12
Liping Qin, Liuqiang Wang, Yong Guo, Yan Li, Halik Ümüt, Yucheng Wang
Ethylene-Responsive Factors (ERFs) are plant-specific transcription factors (TFs) involved in multiple biological processes, especially in abiotic stress tolerance. However, the ERFs from woody halophytes that are involved in salt stress have been little studied. In the present investigation, we characterized a subfamily member of ERF TFs from Tamarix hispida, ThCRF1, which responds to salt stress. ThCRF1 is a nuclear protein that binds to the motifs including TTG, DRE and GCC-box. Transient transformation was performed to generate T...
December 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29223334/expression-of-phytoglobin-affects-nitric-oxide-metabolism-and-energy-state-of-barley-plants-exposed-to-anoxia
#13
Devin W Cochrane, Jay K Shah, Kim H Hebelstrup, Abir U Igamberdiev
Class 1 plant hemoglobins (phytoglobins) are upregulated during low-oxygen stress and participate in metabolism and cell signaling via modulation of the levels of nitric oxide (NO). We studied the effects of overexpression and knockdown of the class 1 phytoglobin gene in barley (Hordeum vulgare L.) under low-oxygen stress. The overexpression of phytoglobin reduced the amount of NO released, while knockdown significantly stimulated NO emission. It has previously been shown that NO inhibits aconitase activity, so decreased aconitase activity in knockdown plants acts as a biomarker for high internal NO levels...
December 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29223333/constitutive-expression-of-brassica-juncea-annexin-annbj2-confers-salt-tolerance-and-glucose-and-aba-insensitivity-in-mustard-transgenic-plants
#14
Israr Ahmed, Deepanker Yadav, Pawan Shukla, T V Vineeth, P C Sharma, P B Kirti
Annexins belong to a plasma membrane binding (in a calcium dependent manner), multi-gene family of proteins, which play ameliorating roles in biotic and abiotic stresses. The expression of annexin AnnBj2 of Indian mustard is tissue specific with higher expression in roots and under treatments with sodium chloride and abscisic acid (ABA) at seedling stage. The effect of constitutive expression of AnnBj2 in mustard was analyzed in detail. AnnBj2 OE (over expression) plants exhibited insensitivity to ABA, glucose and sodium chloride...
December 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29223331/the-nos-like-protein-from-the-microalgae-ostreococcus-tauri-is-a-genuine-and-ultrafast-no-producing-enzyme
#15
Marine Weisslocker-Schaetzel, François André, Nabila Touazi, Noelia Foresi, Mehdi Lembrouk, Pierre Dorlet, Annie Frelet-Barrand, Lorenzo Lamattina, Jérôme Santolini
The exponential increase of genomes' sequencing has revealed the presence of NO-Synthases (NOS) throughout the tree of life, uncovering an extraordinary diversity of genetic structure and biological functions. Although NO has been shown to be a crucial mediator in plant physiology, NOS sequences seem present solely in green algae genomes, with a first identification in the picoplankton species Ostreococcus tauri. There is no rationale so far to account for the presence of NOS in this early-diverging branch of the green lineage and its absence in land plants...
December 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29223330/expression-of-a-populus-histone-deacetylase-gene-84khda903-in-tobacco-enhances-drought-tolerance
#16
Xujun Ma, Bing Zhang, Chunjuan Liu, Botong Tong, Tao Guan, Dean Xia
Histone deacetylases (HDACs) play a key role in regulating plant growth, development and stress responses. However, functions of HDACs in woody plants are largely unknown. In this study, a novel gene encoding a RPD3/HDA1-type histone deacetylase was cloned from 84K poplar (Populus alba×Populus glandulosa) and designated as 84KHDA903. The 84KHDA903 encodes a protein composed of 500 amino acid residues, which contains a conserved HDAC domain. Transient expression of 84KHDA903 in onion epidermal cells suggested that it was exclusively localized in nucleus...
December 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29223138/plant-expressed-fc-fusion-protein-tetravalent-dengue-vaccine-with-inherent-adjuvant-properties
#17
Mi Young Kim, Alastair Copland, Kaustuv Nayak, Anmol Chandele, Muhammad Shamsher Ahmed, Qibo Zhang, Gil Reynolds Diogo, Matthew John Paul, Sven Hofmann, Moon-Sik Yang, Yong-Suk Jang, Julian K-C Ma, Rajko Reljic
Dengue is a major global disease requiring improved treatment and prevention strategies. The recently licensed Sanofi-Pasteur Denvaxia vaccine does not protect children under the age of nine and additional vaccine strategies are thus needed to halt this expanding global epidemic. Here, we employed a molecular engineering approach and plant-expression to produce a humanised and highly immunogenic Poly-Immunoglobulin G Scaffold (PIGS) fused to the consensus dengue envelope protein III domain (cEDIII). The immunogenicity of this IgG Fc receptor targeted vaccine candidate was demonstrated in transgenic mice expressing human FcγRI/CD64, by induction of neutralising antibodies and evidence of cell-mediated immunity...
December 9, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29223068/comprehensive-analysis-and-transcript-profiling-of-arabidopsis-thaliana-and-oryza-sativa-catalase-gene-family-suggests-their-specific-roles-in-development-and-stress-responses
#18
Nazmir Binta Alam, Ajit Ghosh
Stress induces the generation of Reactive Oxygen Species (ROS) that ultimately hampers the growth, development, and productivity of the plant. As an antioxidant enzyme, catalase converts hydrogen peroxide to water and keeps ROS level down to protect cells from stress-induced apoptosis. Here, a genome-wide analysis of catalase gene family has been performed in two model plants- Arabidopsis thaliana and Oryza sativa. Both Arabidopsis and rice has a small family of three and four genes, respectively; that code for seven proteins each...
December 1, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29223066/overexpression-of-the-maize-e3-ubiquitin-ligase-gene-zmairp4-enhances-drought-stress-tolerance-in-arabidopsis
#19
Liang Yang, Lintao Wu, Wei Chang, Zhi Li, Mingjun Miao, Yuejian Li, Junpin Yang, Zhibin Liu, Jun Tan
Ubiquitin-mediated protein degradation plays a crucial role in enabling plants to effectively and efficiently cope with environmental stresses. The E3 ligases have emerged as a central component of the ubiquitination pathway and modulate plant response to abiotic stresses. However, few such studies have been reported in maize. In this study, a C3HC4-type RING finger E3 ligase in maize, ZmAIRP4 (Zea mays Abscisic acid [ABA]-Insensitive RING Protein 4), which is an ortholog of AtAIRP4, was isolated by reverse transcription polymerase chain reaction with specific primers, and its functions in tolerance to drought stress were described...
December 5, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29223065/arabinogalactan-proteins-immunolocalization-in-the-developing-ovary-of-a-facultative-apomict-fragaria-x-ananassa-duch
#20
Agata Leszczuk, Ewa Szczuka
BACKGROUND AND AIMS: Arabinogalactan proteins are present in the extracellular matrix and their occurrence is developmentally regulated. The studies were carried out to localise arabinogalactan proteins in ovary cells of Fragaria x ananassa Duchesne (strawberry) during megasporogenesis, megagametogenesis, and formation of the embryo. METHODS: The research included studies of ovary histochemistry and immunofluorescence labelling of AGP epitopes was performed with antibodies JIM13, JIM15 and MAC207...
December 5, 2017: Plant Physiology and Biochemistry: PPB
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