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Kenji Nemoto, Shuji Oh-ishi, Masato Taguchi, Kentaro Hyodo, Jun Kanazawa, Yukiko Miura, Takio Takaku, Shingo Usui, Kenji Hayashihara, Takefumi Saito
BACKGROUND: T-SPOT.TB (T-SPOT), an interferon-gamma release assay, has shown promise as a diagnostic tool for active tuberculosis (TB), and its use is expanding. Addition of the T-Cell Xtend (TCX) reagent may allow delayed processing, and this characteristic is important for using this test in the field. However, limited data is available on the usefulness of T-SPOT with TCX as a field test for diagnosing active TB. PURPOSE: To investigate the clinical utility of T-SPOT with TCX and the risk factors for a false-negative result in patients with active TB...
April 2016: Kekkaku: [Tuberculosis]
Alfonso Díaz, Samuel Treviño, Jorge Guevara, Guadalupe Muñoz-Arenas, Eduardo Brambila, Blanca Espinosa, Albino Moreno-Rodríguez, Gustavo Lopez-Lopez, Ulises Peña-Rosas, Berenice Venegas, Anabella Handal-Silva, José Luis Morán-Perales, Gonzalo Flores, Patricia Aguilar-Alonso
Energy drinks (EDs) are often consumed in combination with alcohol because they reduce the depressant effects of alcohol. However, different researches suggest that chronic use of these psychoactive substances in combination with alcohol can trigger an oxidative and inflammatory response. These processes are regulated by both a reactive astrogliosis and an increase of proinflammatory cytokines such as IL-1β, TNF-α, and iNOS, causing cell death (apoptosis) at the central and peripheral nervous systems. Currently, mechanisms of toxicity caused by mixing alcohol and ED in the brain are not well known...
2016: Oxidative Medicine and Cellular Longevity
Mariet Allen, Michaela Kachadoorian, Minerva M Carrasquillo, Aditya Karhade, Lester Manly, Jeremy D Burgess, Chen Wang, Daniel Serie, Xue Wang, Joanna Siuda, Fanggeng Zou, High Seng Chai, Curtis Younkin, Julia Crook, Christopher Medway, Thuy Nguyen, Li Ma, Kimberly Malphrus, Sarah Lincoln, Ronald C Petersen, Neill R Graff-Radford, Yan W Asmann, Dennis W Dickson, Steven G Younkin, Nilüfer Ertekin-Taner
OBJECTIVE: To investigate the top late-onset Alzheimer disease (LOAD) risk loci detected or confirmed by the International Genomics of Alzheimer's Project for association with brain gene expression levels to identify variants that influence Alzheimer disease (AD) risk through gene expression regulation. METHODS: Expression levels from the cerebellum (CER) and temporal cortex (TCX) were obtained using Illumina whole-genome cDNA-mediated annealing, selection, extension, and ligation assay (WG-DASL) for ∼400 autopsied patients (∼200 with AD and ∼200 with non-AD pathologies)...
August 2015: Neurology. Genetics
Alice Limonciel, Konrad Moenks, Sven Stanzel, Germaine L Truisi, Céline Parmentier, Lydia Aschauer, Anja Wilmes, Lysiane Richert, Philip Hewitt, Stefan O Mueller, Arno Lukas, Annette Kopp-Schneider, Martin O Leonard, Paul Jennings
High content omic methods provide a deep insight into cellular events occurring upon chemical exposure of a cell population or tissue. However, this improvement in analytic precision is not yet matched by a thorough understanding of molecular mechanisms that would allow an optimal interpretation of these biological changes. For transcriptomics (TCX), one type of molecular effects that can be assessed already is the modulation of the transcriptional activity of a transcription factor (TF). As more ChIP-seq datasets reporting genes specifically bound by a TF become publicly available for mining, the generation of target gene lists of TFs of toxicological relevance becomes possible, based on actual protein-DNA interaction and modulation of gene expression...
December 25, 2015: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Alfonso Díaz, Karla Rojas, Blanca Espinosa, Raúl Chávez, Edgar Zenteno, Daniel Limón, Jorge Guevara
Alzheimer disease (AD) is a neurodegenerative disorder caused by accumulation of the amyloid-beta peptide (Aβ) in neuritic plaques. Its neurotoxic mechanisms are associated with inflammatory responses and nitrosative stress generation that promote expression of inducible nitric oxide synthase (iNOS) and increased nitric oxide causing neuronal death and memory impairment. Studies suggest that treatment with anti-inflammatory and anti-oxidant agents decreases the risk of developing AD. Aminoguanidine (AG) is an iNOS inhibitor with anti-inflammatory and anti-oxidant effects...
June 2014: Neuropeptides
Alfonso Diaz, Daniel Limon, Raúl Chávez, Edgar Zenteno, Jorge Guevara
Amyloid-β (Aβ)25-35 is able to cause memory impairment and neurodegenerative events. Recent evidence has shown that the injection of Aβ25-35 into the temporal cortex (TCx) of rats increases the inflammatory response; however, it is unclear how the inflammatory process could be involved in the progression of Aβ25-35 toxicity. In this study we investigated the role of inflammation in the neuronal damage and spatial memory impairment generated by Aβ25-35 in rat TCx using immunohistochemistry, ELISA, and a behavioral test in the radial maze...
2012: Journal of Alzheimer's Disease: JAD
Glenn Liu, Yu-Hui Chen, Robert Dipaola, Michael Carducci, George Wilding
UNLABELLED: Ixabepilone is an epothilone B analogue with activity in a variety of solid malignancies, including prostate cancer. The main dose-limiting toxicity of ixabepilone is myelosuppression when administered by using an every 3-week schedule. Here we evaluate the activity of a weekly ixabepilone in men with metastatic castrate-resistant prostate cancer to minimize hematologic toxicity. PURPOSE: BMS-247550 (ixabepilone) is an epothilone B analogue with activity in taxane-resistant cancer cell lines...
June 2012: Clinical Genitourinary Cancer
Alfonso Diaz, Liliana Mendieta, Edgar Zenteno, Jorge Guevara, Ilhuicamina Daniel Limon
The Aβ(25-35) fraction mimics the toxic effects of the complete peptide Aβ(1-42) because this decapeptide is able to cause memory impairment and neurodegenerative events. Recent evidence has shown that the injection of Aβ(25-35) into the temporal cortex (TCx) of the rat increases the nitric oxide (NO) pathways with several consequences, such as neuronal loss in rats. Our aim was to investigate the effects of each NOS isoform by the prior injection of NOS inhibitors before the injection of the Aβ(25-35)...
March 2011: Pharmacology, Biochemistry, and Behavior
Frederic Poineau, Erik V Johnstone, Philippe F Weck, Eunja Kim, Paul M Forster, Brian L Scott, Alfred P Sattelberger, Kenneth R Czerwinski
Technetium trichloride has been synthesized by reaction of Tc(2)(O(2)CCH(3))(4)Cl(2) with HCl(g) at 300 °C. The mechanism of formation mimics the one described earlier in the literature for rhenium. Tc(2)(O(2)CCH(3))(2)Cl(4) [P1̅; a = 6.0303(12) Å, b = 6.5098(13) Å, c = 8.3072(16) Å, α = 112.082(2)°, β = 96.667(3)°, γ = 108.792(3)°; Tc-Tc = 2.150(1) Å] is formed as an intermediate in the reaction at 100 °C. Technetium trichloride is formed above 250 °C and is isostructural with its rhenium homologue...
November 17, 2010: Journal of the American Chemical Society
Philippe F Weck, Eunja Kim, Frédéric Poineau, Efrain E Rodriguez, Alfred P Sattelberger, Kenneth R Czerwinski
We report the crystal structures of the novel technetium tetrahalides TcX(4) [X = F, I], as predicted from first-principles calculations. Isomorphous with TcCl(4) and TcBr(4) crystals, TcF(4) is orthorhombic with the centro-symmetric space group Pbca, while TcI(4) crystallizes in the monoclinic space group P2(1)/c. The structures, [TcX(2)(mu-X)(4/2)](infinity), consist of distorted edge-sharing octahedral groups of composition TcX(6) linked into endless cis chains. A possible explanation for the differences between these structures is offered in terms of varying degrees of bonding within the chains...
July 20, 2009: Inorganic Chemistry
Stig Uggerhøj Andersen, Randi Groslier Algreen-Petersen, Martina Hoedl, Anna Jurkiewicz, Cristina Cvitanich, Ulrich Braunschweig, Leif Schauser, Sung-Aeong Oh, David Twell, Erik Østergaard Jensen
Development of reproductive tissue and control of cell division are common challenges to all sexually reproducing eukaryotes. The Arabidopsis thaliana TSO1 gene is involved in both these processes. Mild tso1 mutant alleles influence only ovule development, whereas strong alleles have an effect on all floral tissues and cause cell division defects. The tso1 mutants described so far carry point mutations in a conserved cysteine-rich domain, the CRC domain, but the reason for the range of phenotypes observed is poorly understood...
2007: Journal of Experimental Botany
M J Kieliszewski, A Kamyab, J F Leykam, D T Lamport
Earlier we isolated a threonine-rich extensin from maize (Zea mays). Here, we report that maize cell suspension cultures yield a new extensin rich in histidine (HHRGP) that also has characteristics of arabinogalactan proteins (AGPs). Thus, chymotryptic peptide maps of anhydrous hydrogen fluoride (HF)-deglycosylated HHRGP showed repetitive motifs related to both extensins and AGPs as follows. HHRGP contains Ala-Hyp(3) and Ala-Hyp(4) repeats that may be related to the classical dicot Ser-Hyp(4) extensin motif by the single T --> G (Ser --> Ala) base change...
June 1992: Plant Physiology
Phyllis E Mann, Jessica A Babb
Estrogen and progesterone play important roles during pregnancy in stimulating the onset of maternal behavior at parturition. The status of receptor expression of these hormones during pregnancy in neural regions that regulate maternal behavior is unclear. The objective of the present study is to characterize changes in neural gene expression of the estrogen receptors alpha and beta (ERalpha and ERbeta) and the progesterone receptor (PR) during the latter part of pregnancy. Brains from primigravid Sprague-Dawley rats were collected on days 15 and 21 of pregnancy...
December 7, 2005: Brain Research. Molecular Brain Research
Shaowen Tian, Hua Qi, Jianhong Wang, Jingxia Cai, Yuanye Ma
We simultaneously recorded auditory evoked potentials (AEP) from the temporal cortex (TCx), the dorsolateral prefrontal cortex (dPFCx) and the parietal cortex (PCx) in the freely moving rhesus monkey to investigate state-dependent changes of the AEP. AEPs obtained during passive wakefulness, active wakefulness (AW), slow wave sleep and rapid-eye-movement sleep (REM) were compared. Results showed that AEP from all three cerebral areas were modulated by brain states. However, the amplitude of AEP from dPFCx and PCx significantly appeared greater attenuation than that from the TCx during AW and REM...
October 18, 2002: Neuroscience Letters
R Menze, M J McMullen, L J White, J M Dougherty
OBJECTIVE: To determine core temperature (Tc) elevations in hazardous materials (HazMat) technicians wearing level-A fully encapsulated, chemically resistive suits (FECRSs) during training scenarios. DESIGN: Cross-sectional, observational feasibility study with Institutional Review Board approval. SETTING: HazMat training scenarios held during the summer of 1994. Weather conditions included both rainy and sunny days, with a mean ambient temperature of 75...
April 1996: Prehospital and Disaster Medicine
M P Sauviat
The goal of the present review is to report the effect of the main neurotoxins known on the electrical and mechanical activity of heart muscle. Toxins which block the sodium channel (tetrodotoxin, saxitoxin) shorten the action potential (AP) duration and decrease the initial depolarizing phase of the AP. Toxins which occupy different sites in the channel and alter the gating mechanisms of the Na channel (aconitine, batrachotoxin, veratridine, sea anemone and scorpion toxins, brevetoxin and ciguatoxin) depolarize, lengthen the AP duration, increase the contraction and cause arrhythmias...
1997: Comptes Rendus des Séances de la Société de Biologie et de Ses Filiales
E Fantini, P Athias, R Tirosh, A Pinson
TaiCatoxin (TCX), a complex toxin isolated from Taipan snake venom, is believed to have a specific blocking activity on voltage-dependent cardiac calcium channels. The aim of this study was to investigate the effects of TCX on a broad range of heart muscle cell functions, i.e. electrophysiology, contractility, automaticity and the related biochemical modifications. Myocyte-enriched cultures were prepared from newborn rat heart ventricles. The transmembrane potentials were recorded with glass microelectrodes...
July 1996: Molecular and Cellular Biochemistry
J Cacia, C P Quan, M Vasser, M B Sliwkowski, J Frenz
Chromatographic separations can be tailored to exploit specific interactions between a stationary phase ligand and a protein structural feature of interest. Variations in this feature then form the basis for sorting a mixture of closely related proteins into defined subpopulations. This report describes the sorting of variants of recombinant human deoxyribonuclease I (rhDNase) that differ in the occurrence of deamidation at a single residue. rhDNase, an enzyme that non-specifically hydrolyzes DNA, is glycosylated and exhibits considerable charge heterogeneity owing to the sialylation and phosphorylation of its N-linked oligosaccharides...
March 26, 1993: Journal of Chromatography
K E Linder, Y W Chan, J E Cyr, D P Nowotnik, W C Eckelman, A D Nunn
Several technetium-99 BATO (boronic acid adduct of technetium dioximes) complexes TcX(dioxime)3BR (X = Cl) that contain a boron cap R which bears a 2- or 4-nitroimidazole moiety have been prepared from either TcCl(dioxime)3 or from Tc(dioxime)3(mu-OH)SnCl3 [dioxime = dimethyl glyoxime (DMG) or cyclohexanedione dioxime (CDO)]. Two hydroxy analogs (X = OH) were isolated by treatment of the corresponding chloro complexes with aqueous NaOH. The complexes have been characterized by elemental analysis, mass spectrometry, NMR, UV/vis spectroscopy, and high-performance liquid chromatography...
September 1993: Bioconjugate Chemistry
D R Wallace, R Dawson
Regional regulation of glutaminase by phosphate and calcium was examined in the temporal cortex (TCX), striatum (STR) and hippocampus (HIPP) from adult and aged male F344 rats. Phosphate-dependent glutaminase activity in adult rats was significantly lower (35-43%) in the HIPP (100 and 150 mM) and STR (150 mM) compared to PAG activity in the TCX. Phosphate activation in aged rats was 50-60% lower in the HIPP at concentrations greater than 25 mM compared to the aged TCX or STR. PAG activity in the TCX and STR was unaffected by age, but was significantly reduced (30-50%) in the HIPP from aged rats at phosphate concentrations of 25 mM and greater when compared to adult rats...
December 1993: Neurochemical Research
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