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Mariko Ito, Md Shahin Alam, Mayuko Suzuki, Satoru Takahashi, Miyuki Komura, Natthanan Sangsriratakul, Dany Shoham, Kazuaki Takehara
A quaternary ammonium compound (QAC) was evaluated for its virucidal efficacies with food additive grade calcium hydroxide (FdCa(OH)2 ). When the QAC was diluted 1:500 (QACx500) with redistilled water (dW2 ), it inactivated avian influenza virus (AIV) within 30 sec at 25°C, while at 2°C, it required 1 hr for inactivation. When FdCa(OH)2 powder was added to QACx500 at a final concentration of 0.17%, the mixture, namely Mix500, inactivated AIV within 3 min at 2°C. After contamination with 1% fetal bovine serum (FBS), Mix500 inactivated AIV within 2 hr at 2°C, but QACx500 did not...
March 14, 2018: Journal of Veterinary Medical Science
Breno C B Beirão, Max Ingberman, Celso Fávaro, Dany Mesa, Letícia C Bittencourt, Vitor B Fascina, Luiz Felipe Caron
Probiotics and immunization are being widely adopted by the poultry industry with the goal of controlling Salmonella enterica. However, the interaction between these two management protocols has been sparsely studied. The present study aimed to understand the role of an Enterococcus faecium probiotic in the production of salmonella-specific IgA in layers immunized with a live vaccine. Four groups were used: 'Control' (no vaccine or probiotic); 'Probiotic' (which received an E. faecium product); 'Vaccine' (immunized with two doses of a live attenuated S...
March 14, 2018: Avian Pathology: Journal of the W.V.P.A
Barbara Piccini, Caterina Coviello, Livia Drovandi, Artuso Rosangela, Francesca Monzali, Emilio Casalini, Sabrina Giglio, Sonia Toni, Carlo Dani
Neonatal diabetes mellitus (NDM) is a monogenic form of diabetes occurring within 6 months from birth. NDM can be permanent or transient (TNDM). We report the case of a preterm infant with TNDM due to an ABCC8 mutation identified by next-generation sequencing. The pancreatic adenosine triphosphate (ATP)-sensitive K+ (K-ATP) channel is a key regulator of insulin secretion. Gain-of-function mutations in the genes encoding the Kir6.2 (KCNJ11) and SUR1 (ABCC8) subunits of the channel cause neonatal diabetes. The patient was successfully managed with insulin lispro at a 1:100 dilution, drawn up in an insulin pen injector with a 4-mm needle...
January 2018: American Journal of Perinatology Reports
Zhen Fan, Melanie Dani, Grazia D Femminella, Melanie Wood, Valeria Calsolaro, Mattia Veronese, Federico Turkheimer, Steve Gentleman, David J Brooks, Rainer Hinz, Paul Edison
PURPOSE: Neuroinflammation and microglial activation play an important role in amnestic mild cognitive impairment (MCI) and Alzheimer's disease. In this study, we investigated the spatial distribution of neuroinflammation in MCI subjects, using spectral analysis (SA) to generate parametric maps and quantify11 C-PBR28 PET, and compared these with compartmental and other kinetic models of quantification. METHODS: Thirteen MCI and nine healthy controls were enrolled in this study...
March 9, 2018: European Journal of Nuclear Medicine and Molecular Imaging
Jason C Marvin, Silvia I Gallegos, Shaida Parsaei, Danieli C Rodrigues
PURPOSE: To evaluate the biocompatibility of five dental cement compositions after directly exposing human gingival fibroblast (HGF) and MC3T3-E1 preosteoblast cells to cement alone and cement applied on commercially pure titanium (cpTi) specimens. MATERIALS AND METHODS: Nanostructurally integrated bioceramic (NIB), resin (R), resin-modified glass ionomer (RMGIC), zinc oxide eugenol (ZOE), and zinc phosphate (ZP) compositions were prepared according to the respective manufacturer's instructions...
March 9, 2018: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
Judit Danis, Luca Janovák, Barbara Gubán, Anikó Göblös, Kornélia Szabó, Lajos Kemény, Zsuzsanna Bata-Csörgő, Márta Széll
Keratinocytes are non-professional immune cells contributing actively to innate immune responses partially by reacting to a wide range of molecular patterns by activating pattern recognition receptors. Cytosolic nucleotide fragments as pathogen- or self-derived trigger factors are activating inflammasomes and inducing anti-viral signal transduction pathways as well as inducing expression of inflammatory cytokines. We aimed to compare the induced inflammatory reactions in three keratinocyte cell types-normal human epidermal keratinocytes, the HaCaT cell line and the HPV-KER cell line-upon exposure to the synthetic RNA and DNA analogues poly(I:C) and poly(dA:dT) to reveal the underlying signaling events...
March 8, 2018: International Journal of Molecular Sciences
Ana Carolina Corrêa Café, Carlos Alexandre de Oliveira Lopes, Rommel Larcher Rachid Novais, Wendell Costa Bila, Daniely Karoline da Silva, Márcia Christina Caetano Romano, Joel Alves Lamounier
OBJECTIVE: To systematize literature references addressing the association of sugar-sweetened beverage (SSB) and milk intake with body mass index (BMI) in adolescents. DATA SOURCE: A search was carried out in PubMed (US National Library of Medicine National Institutes of Health) and BVS (Virtual Library in Health). The descriptors used were: adolescents, young adult, beverages, drinking, obesity, overweight, BMI, and nutritional status. The following filters were applied: age ranging from 10 to 19 years, studies published in Portuguese or English language between 2011-2015...
January 2018: Revista Paulista de Pediatria: Orgão Oficial da Sociedade de Pediatria de São Paulo
Lucas Eduardo Trevisoli, Larissa Bail, Luiza Souza Rodrigues, Danieli Conte, Jussara Kasuko Palmeiro, Libera Maria Dalla-Costa
INTRODUCTION.: This study aimed to evaluate different methods for differentiation of species of coagulase-negative staphylococci (CoNS) that caused infections in hospitalized immunocompromised patients. METHODS.: A total of 134 CoNS strains were characterized using four different methods. RESULTS.: The results of matrix assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) analysis were in complete agreement with those of tuf gene sequencing (kappa index = 1...
January 2018: Revista da Sociedade Brasileira de Medicina Tropical
Ely L Steinberg, Yaniv Warschawski, Jacob Elis, Dani Rotman, Gil Rachevsky, Shai Factor, Moshe Salai, Tomer Ben-Tov
AIM: Older patients with proximal femoral fractures often undergo preoperative tests due to coexisting morbidities. Our aim was to evaluate these tests and their impact on patient outcome and medical expenses. METHODS: This retrospective study includes data on head computed tomography, carotid ultrasound, echocardiography and pulmonary functional tests calculated according to the type of surgery (osteosynthesis or hip arthroplasty) carried out on 2798 patients. Time-to-surgery, test repeated postoperatively, American Society of Anesthesiology Physical Status score, additional procedures, hospitalization time, 30-day mortality and associated medical expenses were evaluated...
March 7, 2018: Geriatrics & Gerontology International
Dany H Gagnon, Manuel J Escalona, Martin Vermette, Lívia P Carvalho, Antony D Karelis, Cyril Duclos, Mylène Aubertin-Leheudre
BACKGROUND: For individuals who sustain a complete motor spinal cord injury (SCI) and rely on a wheelchair as their primary mode of locomotion, overground robotic exoskeletons represent a promising solution to stand and walk again. Although overground robotic exoskeletons have gained tremendous attention over the past decade and are now being transferred from laboratories to clinical settings, their effects remain unclear given the paucity of scientific evidence and the absence of large-scale clinical trials...
March 1, 2018: Journal of Neuroengineering and Rehabilitation
Hiroyuki D Sakai, Norio Kurosawa
A novel hyperthermophilic archaeon of strain HS-3T , belonging to the family Sulfolobaceae, was isolated from an acidic terrestrial hot spring in Hakone Ohwaku-dani, Japan. Based on 16S rRNA gene sequence analysis, the closest phylogenetic relatives of strain HS-3T were, first, Sulfolobus solfataricus (96.4 %) and, second, Sulfolobus shibatae (96.2 %), indicating that the strain belongs to the genus Sulfolobus. However, the sequence similarity to the type species of the genus Sulfolobus (Sulfolobus acidocaldarius) was remarkably low (91...
February 27, 2018: International Journal of Systematic and Evolutionary Microbiology
Katalin Paréj, Andrea Kocsis, Csenge Enyingi, Ráhel Dani, Gábor Oroszlán, László Beinrohr, József Dobó, Péter Závodszky, Gábor Pál, Péter Gál
The complement system is a sophisticated network of proteases. In this article, we describe an unexpected link between two linear activation routes of the complement system: the lectin pathway (LP) and the alternative pathway (AP). Mannose-lectin binding-associated serine protease (MASP)-1 is known to be the initiator protease of the LP. Using a specific and potent inhibitor of MASP-1, SGMI-1, as well as other MASP-1 inhibitors with different mechanisms of action, we demonstrated that, in addition to its functions in the LP, MASP-1 is essential for bacterial LPS-induced AP activation, whereas it has little effect on zymosan-induced AP activation...
February 23, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
Piyali Basu, Noor Sanhu, Apoorva Bhatt, Albel Singh, Ricardo Balhana, Irene Gobe, Nicola A Crowhurst, Tom A Mendum, Liang Gao, Jane L Ward, Mike Beale, Johnjoe McFadden, Dany Jv Beste
Enzymes at the PEP-pyruvate-oxaloacetate or anaplerotic (ANA) node control the metabolic flux to glycolysis, gluconeogenesis and anaplerosis. Here we use genetic, biochemical and13 C isotopomer analysis to characterize the role of the enzymes at the ANA node during the intracellular survival of the world's most successful bacterial pathogen, Mycobacterium tuberculosis ( Mtb ). We show that each of the four ANA enzymes, pyruvate carboxylase (PCA), PEP carboxykinase (PCK), malic enzyme (MEZ), and pyruvate phosphate dikinase (PPDK), performs a unique and essential metabolic function during the intracellular survival of Mtb   We show that in addition to PCK, intracellular Mtb requires PPDK as an alternative gateway into gluconeogenesis...
February 23, 2018: Journal of Biological Chemistry
Alini Daniéli Viana Sabino, Cristielli M Camargo, Marcos Hortes N Chagas, Flávia L Osório
Introduction: Music performance anxiety (MPA) can be defined as a lasting and intense apprehension connected with musical performance in public. Studies suggest that MPA can be regarded as a subtype of social anxiety. Since individuals with social anxiety have deficits in the recognition of facial emotion, we hypothesized that musicians with high levels of MPA would share similar impairments. Objective: The aim of this study was to compare parameters of facial emotion recognition (FER) between musicians with high and low MPA...
2018: Frontiers in Psychiatry
Benedict Borer, Robin Tecon, Dani Or
Microbial activity in soil is spatially heterogeneous often forming spatial hotspots that contribute disproportionally to biogeochemical processes. Evidence suggests that bacterial spatial organization contributes to the persistence of anoxic hotspots even in unsaturated soils. Such processes are difficult to observe in situ at the microscale, hence mechanisms and time scales relevant for bacterial spatial organization remain largely qualitative. Here we develop an experimental platform based on glass-etched micrometric pore networks that mimics resource gradients postulated in soil aggregates to observe spatial organization of fluorescently tagged aerobic and facultative anaerobic bacteria...
February 22, 2018: Nature Communications
Kyu-Tae Hwang, Il-Hoon Sung, Jung-Hwan Choi, Jin Kyu Lee
INTRODUCTION: To evaluate the prevalence of medial collateral ligament (MCL) injury of the knee among ankle-fracture patients and to determine the risk factors associated with MCL injury in this patient group. MATERIALS AND METHODS: 303 patients (303 affected ankles) who underwent surgical treatment for an ankle fracture were assessed. Supination versus pronation injury, Danis-Weber classification, age, sex, body mass index (BMI), limb dominance, and mechanism of injury were reviewed to identify factors related to MCL injury...
February 22, 2018: Archives of Orthopaedic and Trauma Surgery
Dany Jaffuel, Nicolas Molinari, Philippe Berdague, Atul Pathak, Michel Galinier, Marion Dupuis, Jean-Etienne Ricci, Jean-Pierre Mallet, Arnaud Bourdin, François Roubille
AIMS: Sleep-disordered breathing (SDB) is a highly prevalent co-morbidity in patients with chronic heart failure (CHF) and can play a detrimental role in the pathophysiology course of CHF. However, the best way to manage SDB in CHF remains a matter of debate. Sacubitril-valsartan has been included in the 2016 European Society of Cardiology guidelines as an alternative to angiotensin-converting enzyme inhibitors to further reduce the risk of progression of CHF, CHF hospitalization, and death in ambulatory patients...
February 22, 2018: ESC Heart Failure
Mark van den Boogaard, Arjen J C Slooter, Roger J M Brüggemann, Lisette Schoonhoven, Albertus Beishuizen, J Wytze Vermeijden, Danie Pretorius, Jan de Koning, Koen S Simons, Paul J W Dennesen, Peter H J Van der Voort, Saskia Houterman, J G van der Hoeven, Peter Pickkers
Importance: Results of studies on use of prophylactic haloperidol in critically ill adults are inconclusive, especially in patients at high risk of delirium. Objective: To determine whether prophylactic use of haloperidol improves survival among critically ill adults at high risk of delirium, which was defined as an anticipated intensive care unit (ICU) stay of at least 2 days. Design, Setting, and Participants: Randomized, double-blind, placebo-controlled investigator-driven study involving 1789 critically ill adults treated at 21 ICUs, at which nonpharmacological interventions for delirium prevention are routinely used in the Netherlands...
February 20, 2018: JAMA: the Journal of the American Medical Association
Iñigo Olalde, Selina Brace, Morten E Allentoft, Ian Armit, Kristian Kristiansen, Thomas Booth, Nadin Rohland, Swapan Mallick, Anna Szécsényi-Nagy, Alissa Mittnik, Eveline Altena, Mark Lipson, Iosif Lazaridis, Thomas K Harper, Nick Patterson, Nasreen Broomandkhoshbacht, Yoan Diekmann, Zuzana Faltyskova, Daniel Fernandes, Matthew Ferry, Eadaoin Harney, Peter de Knijff, Megan Michel, Jonas Oppenheimer, Kristin Stewardson, Alistair Barclay, Kurt Werner Alt, Corina Liesau, Patricia Ríos, Concepción Blasco, Jorge Vega Miguel, Roberto Menduiña García, Azucena Avilés Fernández, Eszter Bánffy, Maria Bernabò-Brea, David Billoin, Clive Bonsall, Laura Bonsall, Tim Allen, Lindsey Büster, Sophie Carver, Laura Castells Navarro, Oliver E Craig, Gordon T Cook, Barry Cunliffe, Anthony Denaire, Kirsten Egging Dinwiddy, Natasha Dodwell, Michal Ernée, Christopher Evans, Milan Kuchařík, Joan Francès Farré, Chris Fowler, Michiel Gazenbeek, Rafael Garrido Pena, María Haber-Uriarte, Elżbieta Haduch, Gill Hey, Nick Jowett, Timothy Knowles, Ken Massy, Saskia Pfrengle, Philippe Lefranc, Olivier Lemercier, Arnaud Lefebvre, César Heras Martínez, Virginia Galera Olmo, Ana Bastida Ramírez, Joaquín Lomba Maurandi, Tona Majó, Jacqueline I McKinley, Kathleen McSweeney, Balázs Gusztáv Mende, Alessandra Mod, Gabriella Kulcsár, Viktória Kiss, András Czene, Róbert Patay, Anna Endrődi, Kitti Köhler, Tamás Hajdu, Tamás Szeniczey, János Dani, Zsolt Bernert, Maya Hoole, Olivia Cheronet, Denise Keating, Petr Velemínský, Miroslav Dobeš, Francesca Candilio, Fraser Brown, Raúl Flores Fernández, Ana-Mercedes Herrero-Corral, Sebastiano Tusa, Emiliano Carnieri, Luigi Lentini, Antonella Valenti, Alessandro Zanini, Clive Waddington, Germán Delibes, Elisa Guerra-Doce, Benjamin Neil, Marcus Brittain, Mike Luke, Richard Mortimer, Jocelyne Desideri, Marie Besse, Günter Brücken, Mirosław Furmanek, Agata Hałuszko, Maksym Mackiewicz, Artur Rapiński, Stephany Leach, Ignacio Soriano, Katina T Lillios, João Luís Cardoso, Michael Parker Pearson, Piotr Włodarczak, T Douglas Price, Pilar Prieto, Pierre-Jérôme Rey, Roberto Risch, Manuel A Rojo Guerra, Aurore Schmitt, Joël Serralongue, Ana Maria Silva, Václav Smrčka, Luc Vergnaud, João Zilhão, David Caramelli, Thomas Higham, Mark G Thomas, Douglas J Kennett, Harry Fokkens, Volker Heyd, Alison Sheridan, Karl-Göran Sjögren, Philipp W Stockhammer, Johannes Krause, Ron Pinhasi, Wolfgang Haak, Ian Barnes, Carles Lalueza-Fox, David Reich
From around 2750 to 2500 bc, Bell Beaker pottery became widespread across western and central Europe, before it disappeared between 2200 and 1800 bc. The forces that propelled its expansion are a matter of long-standing debate, and there is support for both cultural diffusion and migration having a role in this process. Here we present genome-wide data from 400 Neolithic, Copper Age and Bronze Age Europeans, including 226 individuals associated with Beaker-complex artefacts. We detected limited genetic affinity between Beaker-complex-associated individuals from Iberia and central Europe, and thus exclude migration as an important mechanism of spread between these two regions...
February 21, 2018: Nature
Seon-Ah Chong, Silvia Balosso, Catherine Vandenplas, Gregory Szczesny, Etienne Hanon, Kasper Claes, Xavier Van Damme, Bénédicte Danis, Jonathan Van Eyll, Christian Wolff, Annamaria Vezzani, Rafal M Kaminski, Isabelle Niespodziany
Understanding the mechanisms of epileptogenesis is essential to develop novel drugs that could prevent or modify the disease. Neuroinflammation has been proposed as a promising target for therapeutic interventions to inhibit the epileptogenic process that evolves from traumatic brain injury. However, it remains unclear whether cytokine-related pathways, particularly TNFα signaling, have a critical role in the development of epilepsy. In this study, we investigated the role of innate inflammation in an in vitro model of post-traumatic epileptogenesis...
February 20, 2018: Neurotherapeutics: the Journal of the American Society for Experimental NeuroTherapeutics
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