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R Jaisingh, R Shanbhog, B Nandlal, M Thippeswamy
AIM: To evaluate the effect of 10% cerium chloride intervention on pre-demineralised human enamel subjected to cycles of de- and remineralisation. METHODS: This was an in vitro, comparative type study of 60 human enamel samples that were randomly divided into control and test groups. The samples were then subjected to a cycle of demineralisation followed by intervention with respective treatment solutions (control group: placebo solution; test: 10% cerium chloride) for 30 s under constant agitation and then to a 2nd cycle of demineralisation followed by remineralisation using artificial saliva...
March 25, 2017: European Archives of Paediatric Dentistry: Official Journal of the European Academy of Paediatric Dentistry
Luca Golland, Patrick R Schmidlin, Marc Schätzle
PURPOSE: To test the remineralisation potential of a single application of self-assembling peptides or acidic fluoride solution using quantitative light-induced fluorescence (QLF) in vitro. MATERIALS AND METHODS: Bovine enamel disks were prepared, and white spot lesions were created on one half of the disk with an acidic buffer solution. After demineralisation, disks were allocated into three groups of 11 specimens each. Group A served as a control group and received no treatment...
March 20, 2017: Oral Health & Preventive Dentistry
Andrew Yool, Adrian P Martin, Thomas R Anderson, Brian J Bett, Daniel O B Jones, Henry A Ruhl
Deep-water benthic communities in the ocean are almost wholly dependent on near-surface pelagic ecosystems for their supply of energy and material resources. Primary production in sunlit surface waters is channelled through complex food webs that extensively recycle organic material, but lose a fraction as particulate organic carbon (POC) that sinks into the ocean interior. This exported production is further rarefied by microbial breakdown in the abyssal ocean, but a residual ultimately drives diverse assemblages of seafloor heterotrophs...
March 19, 2017: Global Change Biology
Luca Polimene, Darren Clark, Susan Kimmance, Paul McCormack
The capacity of bacteria for degrading dissolved organic nitrogen (DON) and remineralising ammonium is of importance for marine ecosystems, as nitrogen availability frequently limits productivity. Here, we assess the capacity of a widely distributed and metabolically versatile marine bacterium to degrade phytoplankton-derived dissolved organic carbon (DOC) and nitrogen. To achieve this, we lysed exponentially growing diatoms and used the derived dissolved organic matter (DOM) to support an axenic culture of Alteromonas sp...
2017: PloS One
Claudio Poggio, Chiara Gulino, Maria Mirando, Marco Colombo, Giampiero Pietrocola
BACKGROUND: The aim of the present study was to test the impact of different toothpastes with Zinc-Hydroxyapatite (Zn-HAP) on preventing and repairing enamel erosion compared to toothpastes with and without fluoride. MATERIAL AND METHODS: The following four toothpastes were tested: two toothpastes with Zn-HAP, one toothpaste with fluoride and one toothpaste without fluoride. An additional control group was used in which enamel specimens were not treated with toothpaste...
January 2017: Journal of Clinical and Experimental Dentistry
Daniel Wohlgemuth, Martin Solan, Jasmin A Godbold
The ecological consequences of species loss are widely studied, but represent an end point of environmental forcing that is not always realised. Changes in species evenness and the rank order of dominant species are more widespread responses to directional forcing. However, despite the repercussions for ecosystem functioning such changes have received little attention. Here, we experimentally assess how the rearrangement of species dominance structure within specific levels of evenness, rather than changes in species richness and composition, affect invertebrate particle reworking and burrow ventilation behaviour - important moderators of microbial-mediated remineralisation processes in benthic environments - and associated levels of sediment nutrient release...
December 20, 2016: Scientific Reports
N Vyas, R L Sammons, Z Pikramenou, W M Palin, H Dehghani, A D Walmsley
OBJECTIVES: Functionalised silica sub-micron particles are being investigated as a method of delivering antimicrobials and remineralisation agents into dentinal tubules. However, their methods of application are not optimised, resulting in shallow penetration and aggregation. The aim of this study is to investigate the impact of cavitation occurring around ultrasonic scalers for enhancing particle penetration into dentinal tubules. METHODS: Dentine slices were prepared from premolar teeth...
January 2017: Journal of Dentistry
Zeynep Aslı Güçlü, Alev Alaçam, Nichola Jayne Coleman
This 12-week clinical study evaluated the impact of 10% CPP-ACP and 5% sodium fluoride varnish regimes on the regression of nonorthodontic white spot lesions (WSLs). The study included 21 children with 101 WSLs who were randomised into four treatment regimes: weekly clinical applications of fluoride varnish for the first month (FV); twice daily self-applications of CPP-ACP paste (CPP-ACP); weekly applications of fluoride varnish for the first month and twice daily self-applications of CPP-ACP paste (CPP-ACP-FV); and no intervention (control)...
2016: BioMed Research International
Luiza Cassiano, Juliano Pessan, Livia Comar, Flavia Levy, Cristiane Cardoso, Aline Dionisio, Michele Manarelli, Larissa Grizzo, Ana Carolina Magalhães, Marília Buzalaf
OBJECTIVES: This study analysed the effect of frequency of intake and amount of fluoride in milk on the remineralisation of artificial enamel and dentine caries lesions ex vivo/in situ. MATERIALS AND METHODS: Pre-demineralised bovine enamel and dentine slabs were randomly allocated into 5 groups and fixed in removable appliances used by subjects for 7days in each phase. Each treatment comprised milk containing 2.5ppm fluoride daily (T1), or every other day (T2), 5...
January 2017: Archives of Oral Biology
L Paglia
Despite the general advances in dental care, dental caries is still a global health problem affecting many children. Occlusal surfaces of first permanent molars are the most susceptible sites in the developing permanent dentition. Dentists should use sealants or fluoride varnish - as well as other means - to limit the onset of tooth decay. Application of sealants is a recommended procedure to prevent or control caries. Sealing occlusal surfaces of newly erupted permanent molars in children and teenagers delays caries onset up to 48 months compared with unsealed teeth...
September 2016: European Journal of Paediatric Dentistry: Official Journal of European Academy of Paediatric Dentistry
J E Creeth, S A Kelly, C González-Cabezas, R Karwal, E A Martinez-Mier, R J M Lynch, M L Bosma, D T Zero
OBJECTIVES: The influence of toothbrushing duration and dentifrice quantity on fluoride efficacy against dental caries is poorly understood. This study investigated effects of these two oral hygiene factors on enamel remineralisation (measured as surface microhardness recovery [SMHR]), enamel fluoride uptake (EFU), and net acid resistance (NAR) post-remineralisation in a randomized clinical study using an in situ caries model. METHODS: Subjects (n=63) wore their partial dentures holding partially demineralised human enamel specimens and brushed twice-daily for two weeks, following each of five regimens: brushing for 120 or 45s with 1...
December 2016: Journal of Dentistry
Chris Ying Cao, Quan-Li Li
OBJECTIVE: To investigate remineralisation of dentine in the hydrogel microenvironment for the management of hypersensitivity. METHODS: Human dentine slices were prepared from extracted sound human molars. They were acid-etched with phosphoric acid and put into the polyethylene tubes. The etched dentine surfaces were covered by a 2-mm-thick layer of CaCl2 agarose hydrogel. Another 2-mm-thick layer of ion-free agarose hydrogel was added on top of the CaCl2 agarose hydrogel...
October 1, 2016: Journal of Dentistry
I M Makeeva, M A Polyakova, O E Avdeenko, Yu O Paramonov, S A Kondrat'ev, A A Pilyagina
The aim of the study was to evaluate the efficacy of toothpaste «Apadent Total Care» containing nanocalciumhydroxyapatite and its influence on caries resistance of tooth enamel and teeth sensitivity. The study involved 30 people: 15 patients aged 17-25 years and 15 aged 35-44 years. Study participants used «Apadent Total Care» toothpaste with nanocalciumhydroxyapatite. To evaluate the effectiveness of toothpastes clinical assessment of enamel remineralization rate was carried out, as well as the dynamics of enamel acid resistance and teeth sensitivity (Shiff index)...
2016: Stomatologii︠a︡
Deepti Munjal, Shalini Garg, Abhishek Dhindsa, Gagandeep Kaur Sidhu, Harsimran Singh Sethi
INTRODUCTION: As hindrance of remineralisation process occurs during orthodontic therapy resulting in decalcification of enamel because number of plaque retention sites increases due to banding and bonding of appliances to teeth. AIM: The present analytic study was undertaken to assess the occurrence of white spot lesions in permanent molars of children with and without orthodontic therapy and to evaluate the effect of Casein PhosphoPeptide-Amorphous Calcium Phosphate (CPP-ACP) on white spot lesions in post-orthodontic patients in a given period of time...
May 2016: Journal of Clinical and Diagnostic Research: JCDR
Keith J Cross, N Laila Huq, Eric C Reynolds
Tryptic digestion of the calcium-sensitive caseins yields casein phosphopeptides (CPP) that contain clusters of phosphorylated seryl residues. The CPP stabilize calcium and phosphate ions through the formation of complexes. The calcium phosphate in these complexes is biologically available for intestinal absorption and remineralization of subsurface lesions in tooth enamel. We have studied the structure of the complexes formed by the CPP with calcium phosphate using a variety of nuclear magnetic resonance (NMR) techniques...
August 9, 2016: Biochemistry
Rafey Ahmad Jameel, Shah Salman Khan, Zubaidah Hj Abdul Rahim, Marina Mohd Bakri, Saima Siddiqui
OBJECTIVE: To understand early dental erosion induced by different beverages and the equipment for its detection. METHODS: The study was conducted at the University Malaya Medical Centre, Kuala Lumpur, Malaysia, from June to September 2014, and comprised single-rooted, unpolished teeth divided into six groups. Electron micrographs and other baseline readings for further analyses were taken before and after the exposure to different beverages. The teeth were exposed to the beverages using a modified Nordini's artificial mouth model...
July 2016: JPMA. the Journal of the Pakistan Medical Association
Thikrayat Bani Hani, Anne C O'Connell, Brett Duane
Data sourcesMedline, Embase, PreMedline and Cochrane Central Register of Controlled Trials.Study selectionClinical trials, investigating only clinical caries outcomes on participants of any age comparing the use of Tooth Mousse(®) or Tooth Mousse Plus(®) to a routine oral care regimen for the prevention of dental caries with or without comparison to additional preventive products. Studies that used other formulations of CCP-ACP were excluded. Trials using split-mouth design were also excluded. Only English language papers were considered...
2016: Evidence-based Dentistry
Noorjahan Laila Huq, Helen Myroforidis, Keith J Cross, David P Stanton, Paul D Veith, Brent R Ward, Eric C Reynolds
The repair of early dental caries lesions has been demonstrated by the application of the remineralisation technology based on casein phosphopeptide-stabilised amorphous calcium phosphate complexes (CPP-ACP). These complexes consist of an amorphous calcium phosphate mineral phase stabilised and encapsulated by the self-assembly of milk-derived phosphopeptides. During topical application of CPP-ACP complexes in the oral cavity, the CPP encounters the enamel pellicle consisting of salivary proteins and peptides...
June 9, 2016: International Journal of Molecular Sciences
David S Brennan, Madhan Balasubramanian, A John Spencer
OBJECTIVES: Growth in rates of preventive services has been linked to trends in retention of teeth and the emergence of minimal intervention approaches. In this study, we examined associations between patient-level characteristics and rates of the preventive services dental/prophylaxis and application of remineralisation agents. METHODS: A random sample of dentists in Australia was posted a self-administered questionnaire in 2009-2010. A service log was used to collect data on preventive services and patient characteristics...
December 2016: International Dental Journal
Ian D E A Lidbury, Andrew Murphy, David J Scanlan, Gary D Bending, Alexandra M E Jones, Jonathan D Moore, Andrew Goodall, John P Hammond, Elizabeth Wellington
Bacteria that inhabit the rhizosphere of agricultural crops can have a beneficial effect on crop growth. One such mechanism is the microbial-driven solubilisation and remineralisation of complex forms of phosphorus (P). It is known that bacteria secrete various phosphatases in response to low P conditions. However, our understanding of their global proteomic response to P stress is limited. Here, exoproteomic analysis of Pseudomonas putida BIRD-1 (BIRD-1), Pseudomonas fluorescens SBW25 and Pseudomonas stutzeri DSM4166 was performed in unison with whole-cell proteomic analysis of BIRD-1 grown under phosphate (Pi) replete and Pi deplete conditions...
May 27, 2016: Environmental Microbiology
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