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https://www.readbyqxmd.com/read/27894686/the-use-of-the-lymphocyte-cytokinesis-block-micronucleus-assay-for-monitoring-pesticide-exposed-populations
#1
REVIEW
Claudia Bolognesi, Nina Holland
Pesticides are widely used around the world, and hundreds of millions of people are exposed annually in occupational and environmental settings. Numerous studies have demonstrated relationships between pesticide exposure and increased risk of cancers, neurodegenerative and neurodevelopmental disorders, respiratory diseases and diabetes. Assessment of genotoxicity of pesticides and biomonitoring their effect in exposed populations is critical for a better regulation and protection, but it can be complicated because pesticides are often used as complex mixtures...
October 2016: Mutation Research
https://www.readbyqxmd.com/read/27824041/genome-sequence-and-analysis-of-the-japanese-morning-glory-ipomoea-nil
#2
Atsushi Hoshino, Vasanthan Jayakumar, Eiji Nitasaka, Atsushi Toyoda, Hideki Noguchi, Takehiko Itoh, Tadasu Shin-I, Yohei Minakuchi, Yuki Koda, Atsushi J Nagano, Masaki Yasugi, Mie N Honjo, Hiroshi Kudoh, Motoaki Seki, Asako Kamiya, Toshiyuki Shiraki, Piero Carninci, Erika Asamizu, Hiroyo Nishide, Sachiko Tanaka, Kyeung-Il Park, Yasumasa Morita, Kohei Yokoyama, Ikuo Uchiyama, Yoshikazu Tanaka, Satoshi Tabata, Kazuo Shinozaki, Yoshihide Hayashizaki, Yuji Kohara, Yutaka Suzuki, Sumio Sugano, Asao Fujiyama, Shigeru Iida, Yasubumi Sakakibara
Ipomoea is the largest genus in the family Convolvulaceae. Ipomoea nil (Japanese morning glory) has been utilized as a model plant to study the genetic basis of floricultural traits, with over 1,500 mutant lines. In the present study, we have utilized second- and third-generation-sequencing platforms, and have reported a draft genome of I. nil with a scaffold N50 of 2.88 Mb (contig N50 of 1.87 Mb), covering 98% of the 750 Mb genome. Scaffolds covering 91.42% of the assembly are anchored to 15 pseudo-chromosomes...
November 8, 2016: Nature Communications
https://www.readbyqxmd.com/read/27812549/production-of-virus-free-orchid-cymbidium-aloifolium-l-sw-by-various-tissue-culture-techniques
#3
Shreeti Pradhan, Tripti Regmi, Mukunda Ranjit, Bijaya Pant
Orchids are affected by many viruses resulting in poor growth, yield and quality, and an overall decline in population. Cymbidium mosaic virus (CymMV) is one of the common orchid viruses found in Cymbidium species but it infects different orchid genera. In this study Cymbidium aloifolium was propagated in vitro using MS medium at different strength (1.0, ½, and ¼) with or without 0.5 mg/l BAP (6-benzylaminopurine) and 0.5 mg/l NAA (Naphthalene acetic acid). To provide disease-free planting material, source plant for in vitro propagation needs to be screened for pathogenic viruses...
October 2016: Heliyon
https://www.readbyqxmd.com/read/27765992/evaluation-of-steam-and-soil-solarization-for-meloidogyne-arenaria-control-in-florida-floriculture-crops
#4
Nancy Kokalis-Burelle, Erin N Rosskopf, David M Butler, Steven A Fennimore, John Holzinger
Steam and soil solarization were investigated for control of the root-knot nematode Meloidogyne arenaria in 2 yr of field trials on a commercial flower farm in Florida. The objective was to determine if preplant steam treatments in combination with solarization, or solarization alone effectively controlled nematodes compared to methyl bromide (MeBr). Trials were conducted in a field with naturally occurring populations of M. arenaria. Treatments were solarization alone, steam treatment after solarization using standard 7...
September 2016: Journal of Nematology
https://www.readbyqxmd.com/read/27753247/a-perspective-on-management-of-helicoverpa-armigera-transgenic-bt-cotton-ipm-and-landscapes
#5
Sharon Downes, Darren Kriticos, Hazel Parry, Cate Paull, Nancy Schellhorn, Myron P Zalucki
Helicoverpa armigera is a major pest of agriculture, horticulture and floriculture throughout the old world and recently invaded parts of the new world. We overview of the evolution in thinking about the application of area-wide approaches to assist with its control by the Australian Cotton Industry to highlight important lessons and future challenges to achieving the same in the New World. An over-reliance of broad-spectrum insecticides led to Helicoverpa spp. in Australian cotton rapidly became resistant to DDT, synthetic pyrethroids, organophosphates, carbamates and endosulfan...
October 17, 2016: Pest Management Science
https://www.readbyqxmd.com/read/27609470/rnai-induced-silencing-in-floral-tissues-of-petunia-hybrida-by-agroinfiltration-a-rapid-assay-for-chalcone-isomerase-gene-function-analysis
#6
F Keykha, A Bagheri, N Moshtaghi, A R Bahrami, A Sharifi
Variegation in flower color is commonly observed in many plant species and also occurs on Petunia (Petunia hybrida) as an ornamental plant. Variegated plants are of highly valuable in the floricultural market. Agroinfiltration is an Agrobacterium-mediated transient assay for the analysis of gene function and genetic modification in leaves, flowers and fruit tissues of various plants. Transient RNAi-induced silencing by agroinfiltration has been developed in leaves and fruits of several plant species. Here we report the establishment of a transient hairpin RNAi-induced silencing system for color modification assay in floral tissues of Petunia with different colors...
2016: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/27396521/current-status-and-biotechnological-advances-in-genetic-engineering-of-ornamental-plants
#7
REVIEW
Pejman Azadi, Hedayat Bagheri, Ayoub Molaahmad Nalousi, Farzad Nazari, Stephen F Chandler
Cut flower markets are developing in many countries as the international demand for cut flowers is rapidly growing. Developing new varieties with modified characteristics is an important aim in floriculture. Production of transgenic ornamental plants can shorten the time required in the conventional breeding of a cultivar. Biotechnology tools in combination with conventional breeding methods have been used by cut flower breeders to change flower color, plant architecture, post-harvest traits, and disease resistance...
November 1, 2016: Biotechnology Advances
https://www.readbyqxmd.com/read/27385630/in-vitro-evaluation-of-the-mutagenic-and-cytostatic-effect-of-tamaron-lannate-and-manzate-alone-and-in-mixture
#8
Amparo R García-Gutierrez, Reyes Poblano-Bata, Miriam V Flores-Merino, Julieta Castillo-Cadena
Pesticides are often used in agriculture, especially in floriculture. They are frequently applied in binary or ternary mixtures. Nevertheless, their impact on the genetic material has been scarcely explored. In this study, the mutagenic and cytostatic effect of three widely used pesticides, alone and combined, were analyzed. Briefly, lymphocytes cultures were obtained from peripheral blood samples of five healthy donors to determine the sister chromatid exchange and the replicative index (RI). Then, lymphocytes were exposed to Tamaron (100 ppm), Lannate (200 ppm) and Manzate (300 ppm) alone and combined...
November 2016: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/27379163/development-of-ssr-markers-and-assessment-of-genetic-diversity-in-medicinal-chrysanthemum-morifolium-cultivars
#9
Shangguo Feng, Renfeng He, Jiangjie Lu, Mengying Jiang, Xiaoxia Shen, Yan Jiang, Zhi'an Wang, Huizhong Wang
Chrysanthemum morifolium, is a well-known flowering plant worldwide, and has a high commercial, floricultural, and medicinal value. In this study, simple-sequence repeat (SSR) markers were generated from EST datasets and were applied to assess the genetic diversity among 32 cultivars. A total of 218 in silico SSR loci were identified from 7300 C. morifolium ESTs retrieved from GenBank. Of all SSR loci, 61.47% of them (134) were hexa-nucleotide repeats, followed by tri-nucleotide repeats (17.89%), di-nucleotide repeats (12...
2016: Frontiers in Genetics
https://www.readbyqxmd.com/read/27350682/the-first-genetic-linkage-map-of-primulina-eburnea-gesneriaceae-based-on-est-derived-snp-markers
#10
Chen Feng, Chao Feng, Ming Kang
Primulina eburnea is a promising candidate for domestication and floriculture, since it is easy to culture and has beautiful flowers. An F₂ population of 189 individuals was established for the construction of first-generation linkage maps based on expressed sequence tags-derived single-nucleotide polymorphism markers using the massARRAY genotyping platform. Of the 232 screened markers, 215 were assigned to 18 LG according to the haploid number of chromosomes in the species. The linkage map spanned a total of 3774...
June 2016: Journal of Genetics
https://www.readbyqxmd.com/read/27344267/association-between-organochlorine-pesticide-exposure-and-thyroid-hormones-in-floriculture-workers
#11
Julia Blanco-Muñoz, Marina Lacasaña, Inmaculada López-Flores, Miguel Rodríguez-Barranco, Beatriz González-Alzaga, Susana Bassol, Mariano E Cebrian, Lizbeth López-Carrillo, Clemente Aguilar-Garduño
Several studies have suggested that exposure to DDT may be related to changes in thyroid hormone levels in animals and humans, even though results across studies are inconsistent. The aim of this study was to assess the association between exposure to p,p'-DDE (a stable metabolite of DDT) and serum levels of thyroid hormones in floriculture workers. A longitudinal study was conducted on 136 male subjects from the States of Mexico and Morelos, Mexico, who were occupationally exposed to pesticides, during agricultural periods of high (rainy season) and low (dry season) levels of pesticide application...
October 2016: Environmental Research
https://www.readbyqxmd.com/read/27173326/phenotypic-and-biochemical-profile-changes-in-calendula-calendula-officinalis-l-plants-treated-with-two-chemical-mutagenesis
#12
Y I El-Nashar, A A Asrar
Chemical mutagenesis is an efficient tool used in mutation-breeding programs to improve the vital characters of the floricultural crops. This study aimed to estimate the effects of different concentrations of two chemical mutagens; sodium azide (SA) and diethyl sulfate (DES). The vegetative growth and flowering characteristics in two generations (M1 and M2) of calendula plants were investigated. Seeds were treated with five different concentrations of SA and DES (at the same rates) of 1000, 2000, 3000, 4000, and 5000 ppm, in addition to a control treatment of 0 ppm...
2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27145634/a-minimal-cost-micropropagation-protocol-for-dianthus-caryophyllus-l-a-commercially-significant-venture
#13
Manu Pant
In tissue culture, high production cost of the products restricts their reach. Though tissue culture is a major strength in floriculture it is marred by pricing issues. Hence, we developed a complete regeneration low cost micropropagation protocol for an economically important floriculture crop, carnation (Dianthus caryophyllus L.). Successful regeneration of carnation from nodal explants on cost-efficient medium indicates that psyllium husk, sugar and RO water can effectively replace the conventional medium comprising agar, sucrose and distilled water...
March 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/27089475/a-chimeric-repressor-of-petunia-ph4-r2r3-myb-family-transcription-factor-generates-margined-flowers-in-torenia
#14
Ichiro Kasajima, Katsutomo Sasaki
The development of new phenotypes is key to the commercial development of the main floricultural species and cultivars. Important new phenotypes include features such as multiple-flowers, color variations, increased flower size, new petal shapes, variegation and distinctive petal margin colourations. Although their commercial use is not yet common, the transgenic technologies provide a potentially rapid means of generating interesting new phenotypes. In this report, we construct 5 vectors which we expected to change the color of the flower anthocyanins, from purple to blue, regulating vacuolar pH...
May 3, 2016: Plant Signaling & Behavior
https://www.readbyqxmd.com/read/27074980/a-stowaway-transposon-disrupts-the-inwdr1-gene-controlling-flower-and-seed-coloration-in-a-medicinal-cultivar-of-the-japanese-morning-glory
#15
Atsushi Hoshino, Yoshiaki Yoneda, Tsutomu Kuboyama
Floricultural cultivars of the Japanese morning glory (Ipomoea nil) carry transposons of the Tpn1 family as active spontaneous mutagens. Half of the characterized mutations related to floricultural traits were caused by insertion of Tpn1 family elements. In addition, mutations comprising insertions of several bp, presumed to be footprints generated by transposon excisions, were also found. Among these, ca-1 and ca-2 are 7-bp insertions at the same position in the InWDR1 gene, which encodes a multifunctional transcription regulator...
July 20, 2016: Genes & Genetic Systems
https://www.readbyqxmd.com/read/26619872/somatic-embryogenesis-in-lisianthus-eustoma-russellianum-griseb
#16
Barbara Ruffoni, Laura Bassolino
Somatic embryogenesis is, for the main floricultural crops, a promising system for commercial scale-up, providing cloned material to be traded as seedlings. Somatic embryos, having the contemporary presence of root apical meristem and shoot apical meristem, can be readily acclimatized. For Lisianthus it is possible to induce embryogenic callus from leaf fragments of selected genotypes and to obtain embryos either in agarized substrate or in liquid suspension culture. The production of somatic embryos in liquid medium is high and can be modulated in order to synchronize the cycle and the size of the neoformed structures...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/26536625/composition-of-carotenoids-and-flavonoids-in-narcissus-cultivars-and-their-relationship-with-flower-color
#17
Xin Li, Min Lu, Dongqin Tang, Yimin Shi
Narcissus is widely used for cut flowers and potted plants, and is one of the most important commercial bulbous flowers in the floricultural industry. In this study, ten carotenoid and eighteen flavonoid compounds from the perianths and coronas of fifteen narcissus cultivars were measured by HPLC-APCI-MS/MS and UPLC-Q-TOF-MS/MS. Among these, six carotenoids, a total of seventeen flavonols and chlorogenic acid were identified in narcissus for the first time. A multivariate analysis was used to explore the relationship between flower color and pigment composition...
2015: PloS One
https://www.readbyqxmd.com/read/26535657/analysis-of-biochemical-compounds-and-differentially-expressed-genes-of-the-anthocyanin-biosynthetic-pathway-in-variegated-peach-flowers
#18
D Hassani, H L Liu, Y N Chen, Z B Wan, Q Zhuge, S X Li
Variegated plants are highly valuable in the floricultural market, yet the genetic mechanism underlying this attractive phenomenon has not been completely elucidated. In this study, we identified and measured different compounds in pink and white flower petals of peach (Prunus persica) by high-performance liquid chromatography and liquid chromatography/mass spectrometry analyses. No cyanidin-based or pelargonidin-based compounds were detected in white petals, but high levels of these compounds were found in pink petals...
2015: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/26400485/transcriptome-enabled-marker-discovery-and-mapping-of-plastochron-related-genes-in-petunia-spp
#19
Yufang Guo, Krystle E Wiegert-Rininger, Veronica A Vallejo, Cornelius S Barry, Ryan M Warner
BACKGROUND: Petunia (Petunia × hybrida), derived from a hybrid between P. axillaris and P. integrifolia, is one of the most economically important bedding plant crops and Petunia spp. serve as model systems for investigating the mechanisms underlying diverse mating systems and pollination syndromes. In addition, we have previously described genetic variation and quantitative trait loci (QTL) related to petunia development rate and morphology, which represent important breeding targets for the floriculture industry to improve crop production and performance...
2015: BMC Genomics
https://www.readbyqxmd.com/read/26273223/comparative-karyotype-analysis-of-populations-in-the-alstroemeria-presliana-herbert-alstroemeriaceae-complex-in-chile
#20
Carlos Baeza, Víctor L Finot, Eduardo Ruiz
Alstroemeria L., one of the most diverse genera of the Chilean flora and of high floricultural value, is represented by 35 species, most of them distributed between 28-38° S in the Mediterranean zone of Central Chile. There are 24 complex-forming taxa, of which 18 have conservation problems (8 are considered "endangered" and 10 as "vulnerable"). One of these complexes is Alstroemeria presliana Herb. with two subspecies: subsp. presliana and subsp. australis Bayer. Alstroemeria presliana grows in Chile and Argentina: subsp...
May 2015: Genetics and Molecular Biology
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