keyword
MENU ▼
Read by QxMD icon Read
search

Neurosphere

keyword
https://www.readbyqxmd.com/read/29207623/isoxazole-compound-ml327-blocks-myc-expression-and-tumor-formation-in-neuroblastoma
#1
Eric J Rellinger, Chandrasekhar Padmanabhan, Jingbo Qiao, Brian T Craig, Hanbing An, Jing Zhu, Hernán Correa, Alex G Waterson, Craig W Lindsley, R Daniel Beauchamp, Dai H Chung
Neuroblastomas are the most common extracranial solid tumors in children and arise from the embryonic neural crest. MYCN-amplification is a feature of ∼30% of neuroblastoma tumors and portends a poor prognosis. Neural crest precursors undergo epithelial-to-mesenchymal transition (EMT) to gain migratory potential and populate the sympathoadrenal axis. Neuroblastomas are posited to arise due to a blockade of neural crest differentiation. We have recently reported effects of a novel MET inducing compound ML327 (N-(3-(2-hydroxynicotinamido) propyl)-5-phenylisoxazole-3-carboxamide) in colon cancer cells...
October 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/29205371/characterization-of-the-canine-rostral-ventricular-subventricular-zone-morphological-immunohistochemical-ultrastructural-and-neurosphere-assay-studies
#2
Francisco Fernández-Flores, José Manuel García-Verdugo, Raquel Martín-Ibáñez, Cristina Herranz, Dolors Fondevila, Josep María Canals, Carles Arús, Martí Pumarola
The mammalian ventricular-subventricular zone (V-SVZ) presents the highest neurogenic potential in the brain of the adult individual. In rodents, it is mainly composed of chains of neuroblasts. In humans, it is organized in layers where neuroblasts do not form chains. The aim of this study is to describe the cytoarchitecture of canine V-SVZ (cV-SVZ), to assess its neurogenic potential, and to compare our results with those previously described in other species. We have studied by histology, immunohistochemistry (IHC), electron microscopy and neurosphere assay the morphology, cytoarchitecture and neurogenic potential of cV-SVZ...
December 4, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/29203817/high-expression-of-%C3%AE-catenin-contributes-to-the-crizotinib-resistant-phenotype-in-the-stem-like-cell-population-in-neuroblastoma
#3
Abdulraheem Alshareef, Nidhi Gupta, Hai-Feng Zhang, Chengsheng Wu, Moinul Haque, Raymond Lai
ALK has been identified as a novel therapeutic target in neuroblastoma (NB), but resistance to ALK inhibitors (such as crizotinib) is well recognized. We recently published that the crizotinib sensitivity in NB cells strongly correlates with the crizotinib-ALK binding, and β-catenin effectively hinders this interaction and confers crizotinib resistance. Here, we asked if these observations hold true for the stem-like cells in NB cells, which were purified based on their responsiveness to a Sox2 reporter. Compared to bulk, reporter unresponsive (RU) cells, reporter responsive (RR) cells had significantly higher neurosphere formation ability, expression of CD133/nestin and chemo-resistance...
December 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29196262/collagen-vi-suppresses-fibronectin-induced-enteric-neural-crest-cell-migration-by-downregulation-of-focal-adhesion-proteins
#4
Shoichi Nishida, Hisayoshi Yoshizaki, Yoshitomo Yasui, Tsuyoshi Kuwahara, Etsuko Kiyokawa, Miyuki Kohno
The enteric nervous system (ENS) is a network of neurons and glia that are derived from enteric neural crest cells (ENCCs) and essential for regulating peristaltic activity of the colon. ENCCs migrate along the gastrointestinal tract to form the ENS, and disruption of ENCC motility leads to ENS disorders, such as Hirschsprung's disease. Previous ENCC-transplant experiments show that ENCCs can invade into isolated mouse intestines by age E13.5, but not after E15.5. We hypothesized that altered age-specific micro-environments in the intestine are responsible for ENCC invasion/migration...
November 28, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29169175/bufalin-inhibits-cellular-proliferation-and-cancer-stem-cell-like-phenotypes-via-upregulation-of-mir-203-in-glioma
#5
Tao Liu, Chanji Wu, Guohu Weng, Zhongyan Zhao, Xiangying He, Chuanyi Fu, Zhiyan Sui, Shi-Xiong Huang
BACKGROUND/AIMS: Prior studies have shown that bufalin inhibits cellular proliferation and induces apoptosis in various human cancers. MicroRNA-203 (miR-203) has been shown to function as an important regulator of tumor progression at various stages. In this study, we investigated the effect of miR-203 expression and bufalin treatment on glioma cell proliferation and stem cell-like phenotypes. METHODS: We used cell viability assay, colony formation assay, cell apoptosis assay and neurosphere formation assay to dectect the treatment effect of bufalin on U251 and U87 cells...
November 23, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29154990/exosomes-derived-from-human-umbilical-vein-endothelial-cells-promote-neural-stem-cell-expansion-while-maintain-their-stemness-in-culture
#6
Yi-Zhe Zhang, Fan Liu, Chang-Geng Song, Xiu-Li Cao, Yu-Fei Zhang, Hai-Ning Wu, Chen-Jun Guo, Yong-Qiang Li, Qi-Jun Zheng, Min-Hua Zheng, Hua Han
The neural stem cell (NSC) niche in subventricular zone (SVZ) of adult mammalian brain contains dense vascular plexus, where endothelial cells (ECs) regulate NSCs by releasing plenty of angiocrine factors. However, the role of ECs-derived exosomes, a novel type of mediators of intercellular communications, in the regulation of NSCs remains unclear. In the current study, primary NSCs isolated from embryonic mouse brains form more neurospheres when cultured in the presence of human umbilical vein endothelial cells (HUVECs)...
November 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29154838/tp-0903-inhibits-neuroblastoma-cell-growth-and-enhances-the-sensitivity-to-conventional-chemotherapy
#7
Sanja Aveic, Diana Corallo, Elena Porcù, Marcella Pantile, Daniele Boso, Carlo Zanon, Giampietro Viola, Viktoryia Sidarovich, Elena Mariotto, Alessandro Quattrone, Giuseppe Basso, Gian Paolo Tonini
Neuroblastoma (NB) is an embryonal tumor with low cure rate for patients classified as high-risk. This class of NB tumors shows a very complex genomic background and requires aggressive treatment strategies. In this work we evaluated the efficacy of the novel multi-kinase inhibitor TP-0903 in impairing NB cells' growth, proliferation and motility. In vitro studies were performed using cell lines with different molecular background, and in vivo studies were done using the zebrafish experimental model. Our results confirmed a strong cytotoxicity of TP-0903 already at the sub-micro molar concentrations...
November 14, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29152094/xenotransplantation-of-pediatric-low-grade-gliomas-confirms-the-enrichment-of-braf-v600e-mutation-and-preservation-of-cdkn2a-deletion-in-a-novel-orthotopic-xenograft-mouse-model-of-progressive-pleomorphic-xanthoastrocytoma
#8
Mari Kogiso, Lin Qi, Holly Lindsay, Yulun Huang, Xiumei Zhao, Zhigang Liu, Frank K Braun, Yuchen Du, Huiyuan Zhang, Goeun Bae, Sibo Zhao, Sarah G Injac, Mary Sobieski, David Brunell, Vidya Mehta, Diep Tran, Jeffrey Murray, Patricia A Baxter, Xiao-Jun Yuan, Jack M Su, Adekunle Adesina, Laszlo Perlaky, Murali Chintagumpala, D Williams Parsons, Ching C Lau, Clifford C Stephan, Xinyan Lu, Xiao-Nan Li
To identify cellular and molecular changes that driver pediatric low grade glioma (PLGG) progression, we analyzed putative cancer stem cells (CSCs) and evaluated key biological changes in a novel and progressive patient-derived orthotopic xenograft (PDOX) mouse model. Flow cytometric analysis of 22 PLGGs detected CD133(+) (<1.5%) and CD15(+) (20.7 ± 28.9%) cells, and direct intra-cranial implantation of 25 PLGGs led to the development of 1 PDOX model from a grade II pleomorphic xanthoastrocytoma (PXA). While CSC levels did not correlate with patient tumor progression, neurosphere formation and in vivo tumorigenicity, the PDOX model, IC-3635PXA, reproduced key histological features of the original tumor...
October 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/29151265/cnn3-regulates-neural-tube-morphogenesis-and-neuronal-stem-cell-properties
#9
Dirk Junghans, Sebastian Herzog
Calponin 3 (Cnn3) is a member of the calponin family of actin binding molecules that is highly expressed in the mammalian brain and has been shown to control dendritic spine morphology, density and plasticity by regulating actin cytoskeletal reorganization and dynamics. However, little is known about the role of Cnn3 during embryonic development. In this study we analyzed mutant animals deficient in Cnn3 to gain a better understanding of its role in brain morphogenesis. Embryos lacking Cnn3 exhibited massive malformation of the developing brain including exoencephaly, closure defects at the rostral neural tube and strong enlargement of brain tissue...
November 19, 2017: FEBS Journal
https://www.readbyqxmd.com/read/29139540/neurosphere-formation-potential-resides-not-in-the-caudal-cell-mass-but-in-the-secondary-neural-tube
#10
Ji Yeoun Lee, Eun Sun Lee, Saet Pyoul Kim, Myung-Sook Lee, Ji Hoon Phi, Seung-Ki Kim, Young-Il Hwang, Kyu-Chang Wang
The caudal cell mass (CCM) is known as the main player in secondary neurulation, forming the secondary neural tube (2NT). This suggests that the CCM may have the character of neural progenitor cells. The neural potential of the CCM and the 2NT (CCM + 2NT) was assessed by in vitro culture of neurospheres during Hamburger and Hamilton stages (HH) of secondary neurulation (HH16 to HH32). We also analyzed the neural potential of the developing central nervous system (CNS) by comparing the neurosphere culture from the brain, upper / lower spinal cord, and CCM + 2NT from various HH stages...
2017: International Journal of Developmental Biology
https://www.readbyqxmd.com/read/29134074/requirement-of-pax6-for-the-integration-of-guidance-cues-in-cell-migration
#11
Miguel Arocena, Ann M Rajnicek, Jon Martin Collinson
The intricate patterns of cell migration that are found throughout development are generated through a vast array of guidance cues. Responding integratively to distinct, often conflicting, migratory signals is probably crucial for cells to reach their correct destination. Pax6 is a master transcription factor with key roles in neural development that include the control of cell migration. In this study, we have investigated the ability of cells derived from cortical neurospheres from wild-type (WT) and Pax6(-/-) mouse embryos to integrate diverging guidance cues...
October 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/29126932/docosahexaenoic-acid-dha-enhances-the-therapeutic-potential-of-neonatal-neural-stem-cell-transplantation-post-traumatic-brain-injury
#12
Hussein Ghazale, Naify Ramadan, Sara Mantash, Kazem Zibara, Sally El-Sitt, Hala Darwish, Farah Chamaa, Rose Mary Boustany, Stefania Mondello, Wassim Abou-Kheir, Jihane Soueid, Firas Kobeissy
Traumatic Brain Injury (TBI) is a major cause of death and disability worldwide with 1.5 million people inflicted yearly. Several neurotherapeutic interventions have been proposed including drug administration as well as cellular therapy involving neural stem cells (NSCs). Among the proposed drugs is docosahexaenoic acid (DHA), a polyunsaturated fatty acid, exhibiting neuroprotective properties. In this study, we utilized an innovative intervention of neonatal NSCs transplantation in combination with DHA injections in order to ameliorate brain damage and promote functional recovery in an experimental model of TBI...
November 7, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/29116659/type-1-astrocyte-like-stem-cells-harboring-cacna1d-gene-deletion-exhibit-reduced-proliferation-and-decreased-neuronal-fate-choice
#13
Bianca Völkening, Kai Schönig, Golo Kronenberg, Dusan Bartsch, Tillmann Weber
In the central nervous system, CaV 1.2 and CaV 1. 3 constitute the main L-type voltage-gated calcium channels (LTCCs) coupling membrane depolarization to gene transcription. We have previously demonstrated that inducible disruption of Cav1.2 in type-1 astrocyte-like stem cells of the adult dentate gyrus (DG) impairs hippocampal neurogenesis in a cell-autonomous fashion. To address the role of Cav1.3 channels (encoded by the Cacna1d gene), we here generated Tg(GLAST-CreERT2) /Cacna1d(fl/fl) /RCE:loxP mice which facilitate inducible deletion of Cacna1d in tandem with induction of EGFP expression in type-1 cells, allowing tracking of recombined cells and their descendants...
November 8, 2017: Hippocampus
https://www.readbyqxmd.com/read/29115404/downregulation-of-mitochondrial-uqcrb-inhibits-cancer-stem-cell-like-properties-in-glioblastoma
#14
Narae Jung, Ho Jeong Kwon, Hye Jin Jung
Glioblastoma stem cell targeted therapies have become a powerful strategy for the treatment of this deadliest brain tumor. We demonstrate for the first time that downregulation of mitochondrial ubiquinol-cytochrome c reductase binding protein (UQCRB) inhibits the cancer stem cell-like properties in human glioblastoma cells. The synthetic small molecules targeting UQCRB significantly suppressed not only the self-renewal capacity such as growth and neurosphere formation, but also the metastatic potential such as migration and invasion of glioblastoma stem‑like cells (GSCs) derived from U87MG and U373MG at subtoxic concentrations...
November 6, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29112887/comparative-performance-analysis-of-human-ipsc-derived-and-primary-neural-progenitor-cells-npc-grown-as-neurospheres-in-vitro
#15
Maxi Hofrichter, Laura Nimtz, Julia Tigges, Yaschar Kabiri, Friederike Schröter, Brigitte Royer-Pokora, Barbara Hildebrandt, Martin Schmuck, Alexey Epanchintsev, Stephan Theiss, James Adjaye, Jean-Marc Egly, Jean Krutmann, Ellen Fritsche
Developmental neurotoxicity (DNT) testing performed in rats is resource-intensive (costs, time, animals) and bears the issue of species extrapolation. Thus, reliable alternative human-based approaches are needed for predicting neurodevelopmental toxicity. Human induced pluripotent stem cells (hiPSCs) represent a basis for an alternative method possibly being part of an alternative DNT testing strategy. Here, we compared two hiPSC neural induction protocols resulting in 3D neurospheres: one using noggin and one cultivating cells in neural induction medium (NIM protocol)...
October 26, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/29106602/prmt5-as-a-druggable-target-for-glioblastoma-therapy
#16
Yeshavanth Kumar Banasavadi-Siddegowda, Alessandra M Welker, Min An, Xiaozhi Yang, Wei Zhou, Guqin Shi, Jaime Imitola, Chenglong Li, Sigmund Hsu, Jiang Wang, Mitch Phelps, Jianying Zhang, Christine E Beattie, Robert Baiocchi, Balveen Kaur
Background: In spite of standard multi modal therapy consisting of surgical resection followed by radiation and concurrent chemotherapy prognosis for GBM patients remains poor. The identification of both differentiated and undifferentiated "stem cell like" populations in the tumor highlights the significance of finding novel targets that affect the heterogenous tumor cell population. Protein arginine methyltransferase (PRMT5) is one such candidate gene whose nuclear expression correlates with poor survival and has been reported to be required for survival of differentiated GBM cells and self-renewal of undifferentiated GBM cells...
November 2, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/29081002/minocycline-directly-enhances-the-self-renewal-of-adult-neural-precursor-cells
#17
Anri Kuroda, Takahiro Fuchigami, Satoshi Fuke, Natsu Koyama, Kazuhiro Ikenaka, Seiji Hitoshi
Minocycline not only has antibacterial action but also produces a variety of pharmacological effects. It has drawn considerable attention as a therapeutic agent for symptoms caused by inflammation in many neurological disorders, leading to several clinical trials. Although some of these effects are mediated through its function of suppressing microglial activation, it is not clear whether minocycline acts on other cell types in the adult brain. In this study, we utilized a colony-forming neurosphere assay, in which neural stem cells (NSCs) clonally proliferate to form floating colonies, called neurospheres...
October 28, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/29075651/modeling-physiologic-microenvironments-in-three-dimensional-microtumors-maintains-brain-tumor-initiating-cells
#18
Ashley N Gilbert, Kiera Walker, Anh Nhat Tran, Nathaniel H Boyd, G Yancey Gillespie, Raj K Singh, Anita B Hjelmeland
Development of effective novel anti-tumor treatments will require improved in vitro models that incorporate physiologic microenvironments and maintain intratumoral heterogeneity, including tumor initiating cells. Brain tumor initiating cells (BTIC) are a target for cancer therapy, because BTICs are highly tumorigenic and contribute to tumor angiogenesis, invasion, and therapeutic resistance. Current leading studies rely on BTIC isolation from patient-derived xenografts followed by propagation as neurospheres...
2017: Journal of Cancer Stem Cell Research
https://www.readbyqxmd.com/read/29067692/glucocorticoids-promote-a-glioma-stem-cell-like-phenotype-and-resistance-to-chemotherapy-in-human-glioblastoma-primary-cells-biological-and-prognostic-significance
#19
Ourania N Kostopoulou, Abdul-Aleem Mohammad, Jiri Bartek, Julia Winter, Masany Jung, Giuseppe Stragliotto, Cecilia Söderberg-Nauclér, Natalia Landázuri
Glioma stem cells (GSCs) are glioblastoma (GBM) cells that are resistant to therapy and can give rise to recurrent tumors. The identification of patient-related factors that support GSCs is thus necessary to design effective therapies for GBM patients. Glucocorticoids (GCs) are used to treat GBM-associated edema. However, glucocorticoids participate in the physiological response to psychosocial stress, which has been linked to poor cancer prognosis. This raises concern that glucocorticoids affect the tumor and GSCs...
October 25, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29042487/differential-response-of-glioma-stem-cells-to-arsenic-trioxide-therapy-is-regulated-by-mnk1-and-mrna-translation
#20
Jonathan B Bell, Frank Eckerdt, Harshil Dhruv, Darren Finlay, Sen Peng, Seungchan Kim, Barbara Kroczynska, Elspeth Beauchamp, Kristen Alley, Jessica Clymer, Stewart Goldman, Shi-Yuan Cheng, C David James, Ichiro Nakano, Craig Horbinski, Andrew P Mazar, Kristiina Vuori, Priya Kumthekar, Jeffery Raizer, Michael E Berens, Leonidas C Platanias
Mesenchymal (MES) and proneural (PN) are two distinct glioma stem cells (GSCs) populations that drive therapeutic resistance in glioblastoma (GBM). We screened a panel of 650 small molecules against patient-derived GBM cells to discover compounds targeting specific GBM subtypes. Arsenic trioxide (ATO), a FDA-approved drug that crosses the blood-brain barrier, was identified as a potent PN-specific compound in the initial screen and follow-up validation studies. Furthermore, MES and PN GSCs exhibited differential sensitivity to ATO...
October 17, 2017: Molecular Cancer Research: MCR
keyword
keyword
93530
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"