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Sputum cytology

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https://www.readbyqxmd.com/read/29453637/cough-hypersensitivity-in-patients-with-obstructive-sleep-apnea-hypopnea-syndrome
#1
Cuiqin Shi, Siwei Liang, Xianghuai Xu, Qiang Chen, Lan Wang, Li Yu, Hanjing Lv, Zhongmin Qiu
PURPOSE: The purpose of this study was to investigate cough hypersensitivity and its potential mechanisms in patients with obstructive sleep apnea hypopnea syndrome (OSAHS). METHODS: Fifteen OSAHS patients, 12 simple snoring patients, and 15 healthy volunteers received cough sensitivity test and induced sputum cytology. Cough thresholds C2 and C5 (the minimum of capsaicin inducing ≥ 2 and ≥ 5 coughs, respectively), total cell count, cell differentials and the levels of bradykinin, histamine, prostaglandin E 2 , substance P, calcitonin gene-related peptide, pepsin, and interleukin-2 in the induced sputum detected by enzyme-linked immunosorbent assay were compared...
February 16, 2018: Sleep & Breathing, Schlaf & Atmung
https://www.readbyqxmd.com/read/29436153/advanced-imaging-technology-applications-in-cytology
#2
Liron Pantanowitz, Frederic Preffer, David C Wilbur
Novel techniques have been developed to image cells at cellular and subcellular levels. They allow images to be analyzed with ultra-high resolution, in 2D and/or 3D. Several of these tools have been tested on cytology specimens demonstrating emerging applications that are likely to change the field of cytopathology. This review covers several of these advanced imaging methods. The use of optical coherence tomography to perform optical biopsies during endoscopic ultrasound procedures or visualize cells within effusion samples is discussed...
February 12, 2018: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/29417838/lung-cancer-a-brief-review-of-epidemiology-and-screening
#3
Cláudia Gouvinhas, Ramon Andrade De Mello, Daniela Oliveira, José Manuel Castro-Lopes, Pedro Castelo-Branco, Ricardo Sales Dos Santos, Venceslau Hespanhol, Daniel Humberto Pozza
The global burden of lung cancer has been increasing over the past years, and is still a major threat to public health worldwide, leading to disabilities and premature mortality. Despite multifactorial cause, smoking remains as the major etiological factor, followed by occupational exposure to carcinogens, genetic predisposition and other concomitant diseases. In order to reduce the individual and social burden due to the direct and indirect costs related to the lung cancer treatment, accurate methods of screening are needed...
February 8, 2018: Future Oncology
https://www.readbyqxmd.com/read/29403171/sputum-cytology-curschmann-s-spiral-or-parasite-larva
#4
Rafael Martínez-Girón, Cristina Martínez-Torre
No abstract text is available yet for this article.
January 2018: Journal of Cytology
https://www.readbyqxmd.com/read/29395563/-bacteriology-of-acute-exacerbations-of-chronic-obstructive-pulmonary-disease-in-tunisia
#5
S Messous, M H Grissa, K Beltaief, R Boukef, S Nouira, M Mastouri
INTRODUCTION: The role of bacteria, including atypical organisms, in acute exacerbations of chronic obstructive pulmonary disease (AECOPD) has been assessed in various ways in Tunisia. METHODS: This was a descriptive and analytical study of patients with a mean age of 68.3±10.5 years hospitalized for AECOPD. Bacteriological examination included a cytological sputum exam and serology for atypical organisms including Mycoplasma pneumoniae, Coxiella burnetii, Chlamydophila pneumoniae and Legionella pneumophila using standard techniques...
January 29, 2018: Revue des Maladies Respiratoires
https://www.readbyqxmd.com/read/29368894/-airway-metaplasia-related-to-smoking-and-environmental-pollution-through-sputum
#6
Floribel Ferman-Cano, Fernando Padilla-Santamaría, Luis Moreno-Venegas, Carlos Alejandro Torner-Aguilar, Miguel Angel Molina-Medina
BACKGROUND: Both environmental pollution and smoking affect the respiratory epithelium, causing cellular adaptation changes. Therefore, this work explores the presence of metaplasia in smokers compared with non-smokers from Mexico City. METHODS: A prospective cohort study was performed. The sample was performed through a home interview covering the five Mexico City zones delimited to the study and monitoring of air quality. We searched metaplastic cells and inflammation in sputum cytology stained with Papanicolaou technique, and we assessed the air quality in Mexico City...
January 2018: Revista Médica del Instituto Mexicano del Seguro Social
https://www.readbyqxmd.com/read/29363060/inflammation-oxidative-stress-and-higher-expression-levels-of-nrf2-and-nqo1-proteins-in-the-airways-of-women-chronically-exposed-to-biomass-fuel-smoke
#7
Nandan Kumar Mondal, Hirak Saha, Bidisha Mukherjee, Neetu Tyagi, Manas Ranjan Ray
The study was carried out to examine whether chronic exposure to smoke during daily household cooking with biomass fuel (BMF) elicits changes in airway cytology and expressions of Nrf2 (nuclear factor erythroid 2 [NF-E2]-related factor 2 [Nrf2]), Keap1 (Kelch-like erythroid-cell-derived protein with CNC homology [ECH]-associated protein 1), and NQO1 (NAD(P)H:quinone oxidoreductase 1) proteins in the airways. For this, 282 BMF-using women (median age 34 year) and 236 age-matched women who cooked with liquefied petroleum gas (LPG) were enrolled...
January 24, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29337345/sputum-cytology-during-late-phase-responses-to-inhalation-challenge-with-different-allergens
#8
Joana A Revez, Kieran J Killian, Paul M O'Byrne, Louis-Philippe Boulet, John W Upham, Gail M Gauvreau, Manuel A R Ferreira
BACKGROUND: In mouse models of allergic asthma, exposure to different allergens can trigger distinct inflammatory subtypes in the airways. We investigated if this observation extends to humans. METHODS: We compared the frequency of sputum inflammatory subtypes between mild allergic asthma subjects (n=129) exposed to different allergens in inhalation challenge tests. These tests were performed using a standardized protocol as part of clinical trials of experimental treatments for asthma, prior to drug randomization...
January 16, 2018: Allergy
https://www.readbyqxmd.com/read/29172277/dna-replication-licensing-proteins-for-early-detection-of-lung-cancer
#9
Veena V S, Rajan K, Saritha V N, Preethi Sara George, Chandramohan K, Jayasree K, Thara S, K Sujathan
Background: To identify and characterize malignant and premalignant cells in sputum and matched tissue samples with reference to expression of minichromosome maintenance proteins (MCM2, MCM5) and cell division cycle protein 6 (CDC 6) and to assess their potential as biomarkers of premalignant and malignant lesions of the lung and associations with clinicopathological features. Methods: Expression of MCM2, MCM5 and 6 proteins in sputum samples and corresponding tissues was assessed by immunocytochemistry, and correlated with histological findings...
November 26, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/29108319/detection-of-tumor-derived-dna-dispersed-in-the-airway-improves-the-diagnostic-accuracy-of-bronchoscopy-for-lung-cancer
#10
Taichiro Goto, Yosuke Hirotsu, Takahiro Nakagomi, Daichi Shikata, Yujiro Yokoyama, Kenji Amemiya, Toshiharu Tsutsui, Yumiko Kakizaki, Toshio Oyama, Hitoshi Mochizuki, Yoshihiro Miyashita, Masao Omata
The diagnostic accuracy of bronchoscopy for detecting lung cancer, especially peripheral lung cancer with lesions outside the endoscopically visible range, remains unsatisfactory. The aim of this study was to perform next-generation sequencing on bronchoscopic specimens to determine whether this improves the accuracy of bronchoscopy for diagnosing lung cancer and to identify factors influencing sensitivity. The bronchoscopic sensitivity for diagnosing lung cancer was initially evaluated in 191 patients who underwent lobectomy after bronchoscopy at our hospital...
October 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/29065541/screening-and-biosensor-based-approaches-for-lung-cancer-detection
#11
REVIEW
Lulu Wang
Early diagnosis of lung cancer helps to reduce the cancer death rate significantly. Over the years, investigators worldwide have extensively investigated many screening modalities for lung cancer detection, including computerized tomography, chest X-ray, positron emission tomography, sputum cytology, magnetic resonance imaging and biopsy. However, these techniques are not suitable for patients with other pathologies. Developing a rapid and sensitive technique for early diagnosis of lung cancer is urgently needed...
October 23, 2017: Sensors
https://www.readbyqxmd.com/read/28942912/an-evolving-role-for-cancer-rehabilitation-in-the-era-of-low-dose-lung-computed-tomography-screening
#12
REVIEW
Sean Robinson Smith, Ashish Khanna, Eric M Wisotzky
Lung cancer is the number one cause of cancer-related death worldwide, and is often detected in the later stages. Use of low-dose chest computed tomography in at-risk patients provides earlier detection and is being adopted as the standard screening tool, replacing less precise methods of radiography and sputum cytology. In the past, late detection of disease meant that rehabilitation interventions attempted to salvage function and to improve aerobic capacity to the point where patients could tolerate the sometimes-extensive oncologic treatment, including lobectomy or pneumonectomy...
September 2017: PM & R: the Journal of Injury, Function, and Rehabilitation
https://www.readbyqxmd.com/read/28914465/exploring-avenues-for-best-use-of-cytotechnologists-in-non-gynaecological-cytology-double-screening-or-independent-sign-out
#13
G Fischer, M Haddad, K Cormier
OBJECTIVE: Cytotechnologist (CT) screening workload has been decreasing due to the falling number of Papanicolaou tests. This continuing trend has prompted exploration of ways to best employ the CT skillset. One potential way of more effective use is by having two CTs double screen non-gynaecological (NGC) cases to assess whether this improves screening quality and concordance with pathologists. Another is evaluating the CT's performance on low-complexity negative NGC cases for a potential independent CT sign-out without pathologist review...
September 15, 2017: Cytopathology: Official Journal of the British Society for Clinical Cytology
https://www.readbyqxmd.com/read/28900554/primary-epithelioid-angiosarcoma-of-lung-radiologic-and-clinicopathologic-correlation
#14
Fung Him Ng, San Ming Yu, Ophelia Ka Heng Wai, James Chi Sang Chan
Primary pulmonary angiosarcoma is extremely rare. It is often characterized by a clinically indolent course and delayed diagnosis. To date, there have been <20 cases reported. By far, little article correlates the clinical presentation, the imaging findings with the pathology. The authors present a case of middle-aged gentleman with primary pulmonary epithelioid angiosacroma which we initially thought as tuberculosis (TB) infection. A 60-year-old gentleman, with a history of 6 months on and off blood stained sputum, was admitted for an episode of massive hemoptysis...
2017: Journal of Clinical Imaging Science
https://www.readbyqxmd.com/read/28790413/liquid-based-cytopathology-test-as-a-novel-method-to-identify-aspergillus-in-patients-with-pulmonary-aspergillosis
#15
Yongchun Shen, Xiaorong Zhang, Wenyi Lin, Chun Wan, Qiyuan Li, Yong Jiang
Conventional cytopathology examination of respiratory samples can aid in identifying Aspergillus but with poor sensitivity, so this study aimed to assess the potential of the liquid-based cytopathology test (LCT) for improving the identification of Aspergillus in respiratory samples following Papanicolaou's or Special staining with Grocott's methenamine silver or periodic acid-Schiff staining. Paired bronchial brushing samples (n = 54) and sputum samples (n = 117) from 171 patients with pulmonary aspergillosis were prepared as slides using either conventional cytopathology or SurePath LCT...
August 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28733314/effects-of-an-airway-clearance-device-on-inflammation-bacteriology-and-mucus-transport-in-bronchiectasis
#16
Joana Tambascio, Hugo Celso Dutra de Souza, Roberto Martinez, José Antônio Baddini-Martinez, Peter John Barnes, Ada Clarice Gastaldi
BACKGROUND: Bronchiectasis is characterized by abnormal and permanent dilation of the bronchi, caused by the perpetuation of inflammation and impairment of mucociliary clearance. Physiotherapy techniques can help to decrease the retention of respiratory secretions. The flutter valve combines high-frequency oscillation and positive expiratory pressure to facilitate the removal of secretions. We evaluated the effects of the flutter valve on sputum inflammation, microbiology, and transport of respiratory secretions in patients with bronchiectasis...
August 2017: Respiratory Care
https://www.readbyqxmd.com/read/28637439/a-qualitative-analysis-of-smokers-perceptions-about-lung-cancer-screening
#17
Lindsay Gressard, Amy S DeGroff, Thomas B Richards, Stephanie Melillo, Julia Kish-Doto, Christina L Heminger, Elizabeth A Rohan, Kristine Gabuten Allen
BACKGROUND: In 2013, the US Preventive Services Task Force (USPSTF) began recommending lung cancer screening for high risk smokers aged 55-80 years using low-dose computed tomography (CT) scan. In light of these updated recommendations, there is a need to understand smokers' knowledge of and experiences with lung cancer screening in order to inform the design of patient education and tobacco cessation programs. The purpose of this study is to describe results of a qualitative study examining smokers' perceptions around lung cancer screening tests...
June 21, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28596492/detection-of-tumor-derived-dna-dispersed-in-the-airway-improves-the-diagnostic-accuracy-of-bronchoscopy-for-lung-cancer
#18
Taichiro Goto, Yosuke Hirotsu, Takahiro Nakagomi, Daichi Shikata, Yujiro Yokoyama, Kenji Amemiya, Toshiharu Tsutsui, Yumiko Kakizaki, Toshio Oyama, Hitoshi Mochizuki, Yoshihiro Miyashita, Masao Omata
The diagnostic accuracy of bronchoscopy for detecting lung cancer, especially peripheral lung cancer with lesions outside the endoscopically visible range, remains unsatisfactory. The aim of this study was to perform next-generation sequencing on bronchoscopic specimens to determine whether this improves the accuracy of bronchoscopy for diagnosing lung cancer and to identify factors influencing sensitivity. The bronchoscopic sensitivity for diagnosing lung cancer was initially evaluated in 191 patients who underwent lobectomy after bronchoscopy at our hospital...
May 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/28535501/is-the-morning-sputum-sample-superior-to-the-fresh-sputum-sample-for-the-detection-of-malignant-cells
#19
Maya S Uke, Saleem Pathuthara, Abida Shaikh, Rajiv Kumar, Shubhada Kane
INTRODUCTION: Sputum cytology is a well-established technique for the detection of lung malignancies. Generally, random or morning samples are used. OBJECTIVES: To evaluate the diagnostic yield of morning sputum and compare it with that of fresh samples. MATERIALS AND METHODS: Patients were instructed to bring a morning sputum sample to the laboratory in a clean plastic container, without any fixative and within 2-3 h of collection. Fresh sputum was then collected in the laboratory from these same patients...
2017: Acta Cytologica
https://www.readbyqxmd.com/read/28410823/the-case-for-performing-pleural-biopsies-for-the-aetiological-diagnosis-of-exudates-yes
#20
F Rodriguez-Panadero
Pleural biopsies are especially indicated in the following circumstances: a) inconclusive pleural fluid analysis and negative sputum study, if adenosine deaminase (ADA) levels are unavailable; b) suspected multi-resistant tuberculosis; c) a need for differentiating tuberculous pleurisy (if it progresses with neutrophilia) and complicated parapneumonic effusion; d) malignant pleural effusion coexisting with very high ADA levels; e) effusion coexisting with lung cancer and negative pleural cytology; f) suspected mesothelioma; and g) need for implementing re-treatment for patients with relapse after chemotherapy...
October 2017: Revista Clínica Española
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