Yi Li, Zixin Liu, Ximeng Han, Feng Liang, Qianyu Zhang, Xiuzhen Huang, Xin Shi, Huanhuan Huo, Maoying Han, Xiuxiu Liu, Huan Zhu, Lingjuan He, Linghong Shen, Xinyang Hu, Jian'an Wang, Qing-Dong Wang, Nicola Smart, Bin Zhou, Ben He
BACKGROUND: Endothelial cell (EC) generation and turnover by self-proliferation contributes to vascular repair and regeneration. The ability to accurately measure the dynamics of EC generation would advance our understanding of cellular mechanisms of vascular homeostasis and diseases. However, it is currently challenging to evaluate the dynamics of EC generation in large vessels such as arteries because of their infrequent proliferation. METHODS: By using dual recombination systems based on Cre-loxP and Dre-rox, we developed a genetic system for temporally seamless recording of EC proliferation in vivo...
December 12, 2023: Circulation