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https://www.readbyqxmd.com/read/27881992/molecular-mapping-and-qtl-for-expression-profiles-of-flavonoid-genes-in-brassica-napus
#1
Cunmin Qu, Huiyan Zhao, Fuyou Fu, Kai Zhang, Jianglian Yuan, Liezhao Liu, Rui Wang, Xinfu Xu, Kun Lu, Jia-Na Li
Flavonoids are secondary metabolites that are extensively distributed in the plant kingdom and contribute to seed coat color formation in rapeseed. To decipher the genetic networks underlying flavonoid biosynthesis in rapeseed, we constructed a high-density genetic linkage map with 1089 polymorphic loci (including 464 SSR loci, 97 RAPD loci, 451 SRAP loci, and 75 IBP loci) using recombinant inbred lines (RILs). The map consists of 19 linkage groups and covers 2775 cM of the B. napus genome with an average distance of 2...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27860412/cryo-protective-effect-of-an-ice-binding-protein-derived-from-antarctic-bacteria
#2
Marco Mangiagalli, Maya Bar-Dolev, Pietro Tedesco, Antonino Natalello, Aleksei Kaleda, Stefania Brocca, Donatella de Pascale, Sandra Pucciarelli, Cristina Miceli, Ido Bravslavsky, Marina Lotti
Cold environments are populated by organisms able to contravene deleterious effects of low temperature by diverse adaptive strategies, including the production of ice binding proteins (IBPs) that inhibit the growth of ice crystals inside and outside cells. We describe the properties of such a protein (EfcIBP) identified in the metagenome of an Antarctic biological consortium composed by the ciliate Euplotes focardii and psychrophilic non-cultured bacteria. Recombinant EfcIBP can resist freezing without any conformational damage and is moderately heat stable, with a Tm of 66...
November 17, 2016: FEBS Journal
https://www.readbyqxmd.com/read/27835056/multispectroscopic-studies-on-the-interaction-of-a-copper-ii-complex-of-ibuprofen-drug-with-calf-thymus-dna
#3
Nahid Shahabadi, Farshad Shiri
The interaction of copper(II)-ibuprofenato complex with calf thymus DNA (ct-DNA) has been explored following, UV-visible spectrophotometry, fluorescence measurement, dynamic viscosity measurements, and circular dichroism spectroscopy. In spectrophotometric studies of ct-DNA it was found that [Cu(ibp)2]2 can form a complex with double-helical DNA. The association constant of [Cu(ibp)2]2 with DNA from UV-Vis study was found to be 6.19 × 10(4) L mol(-1). The values of Kf from fluorescence measurement clearly underscore the high affinity of [Cu(ibp)2]2 to DNA...
November 11, 2016: Nucleosides, Nucleotides & Nucleic Acids
https://www.readbyqxmd.com/read/27809783/transcriptomic-data-from-panarthropods-shed-new-light-on-the-evolution-of-insulator-binding-proteins-in-insects-insect-insulator-proteins
#4
Thomas Pauli, Lucia Vedder, Daniel Dowling, Malte Petersen, Karen Meusemann, Alexander Donath, Ralph S Peters, Lars Podsiadlowski, Christoph Mayer, Shanlin Liu, Xin Zhou, Peter Heger, Thomas Wiehe, Lars Hering, Georg Mayer, Bernhard Misof, Oliver Niehuis
BACKGROUND: Body plan development in multi-cellular organisms is largely determined by homeotic genes. Expression of homeotic genes, in turn, is partially regulated by insulator binding proteins (IBPs). While only a few enhancer blocking IBPs have been identified in vertebrates, the common fruit fly Drosophila melanogaster harbors at least twelve different enhancer blocking IBPs. We screened recently compiled insect transcriptomes from the 1KITE project and genomic and transcriptomic data from public databases, aiming to trace the origin of IBPs in insects and other arthropods...
November 3, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27782870/factors-associated-with-the-decision-of-the-rheumatologist-to-order-sacroiliac-joints-magnetic-resonance-imaging-si-mri-or-hla-b27-testing-in-the-diagnostic-work-up-of-patients-with-spondyloarthritis-in-clinical-practice
#5
Wilson Bautista-Molano, Robert B M Landewé, Cesar Serna, Rafael Valle-Oñate, Désirée van der Heijde
OBJECTIVES: To evaluate the patients' characteristics associated with the clinical decision to request SI-MRI and/or HLA-B27 in patients with SpA in daily practice. METHODS: Patients referred to a rheumatology outpatient-clinic in a national referral-centre were selected. Patients with a clinical diagnosis of SpA according to the rheumatologist were included. SI-MRI and HLA-B27 was available for patients in whom the rheumatologists had ordered these tests. Characteristics associated with ordering SI-MRI or HLA-B27 were identified with univariable analyses...
October 26, 2016: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/27773244/magnetic-pd-fe3o4-composite-nanostructure-as-recoverable-catalyst-for-sonoelectrohybrid-degradation-of-ibuprofen
#6
Binota Thokchom, Pengpeng Qiu, Mingcan Cui, Beomguk Park, Aniruddha B Pandit, Jeehyeong Khim
In the present research, the degradation of an emerging pharmaceutical micro-pollutant, Ibuprofen (IBP) by using Pd@Fe3O4 and a hybrid sono-electrolytical (US/EC) treatment system has been demonstrated for the first time. The magnetically separable nanocomposite, Pd@Fe3O4 catalyst was synthesized following co-precipitation method to enhance the efficiency of US/EC system. The synthesized catalyst showed a strong reusable property even after applying for five times and in all the five cases, 100% degradation of IBP was maintained...
January 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/27763509/electrochemical-selective-and-simultaneous-detection-of-diclofenac-and-ibuprofen-in-aqueous-solution-using-hkust-1-metal-organic-framework-carbon-nanofiber-composite-electrode
#7
Sorina Motoc, Florica Manea, Adriana Iacob, Alberto Martinez-Joaristi, Jorge Gascon, Aniela Pop, Joop Schoonman
In this study, the detection protocols for the individual, selective, and simultaneous determination of ibuprofen (IBP) and diclofenac (DCF) in aqueous solutions have been developed using HKUST-1 metal-organic framework-carbon nanofiber composite (HKUST-CNF) electrode. The morphological and electrical characterization of modified composite electrode prepared by film casting was studied by scanning electronic microscopy and four-point-probe methods. The electrochemical characterization of the electrode by cyclic voltammetry (CV) was considered the reference basis for the optimization of the operating conditions for chronoamperometry (CA) and multiple-pulsed amperometry (MPA)...
October 17, 2016: Sensors
https://www.readbyqxmd.com/read/27750164/ibuprofen-degradation-and-toxicity-evolution-during-fe-2-oxone-uv-process
#8
Han Gong, Wei Chu, So Hiu Lam, Angela Yu-Chen Lin
This study shows the degradation of ibuprofen in aqueous solution using oxone process mediated by Fe(2+) with UV irradiation (FOU). Fe(2+)/Oxone (FO), Fe(2+)/UV (FU), Oxone/UV (OU) processes were investigated separately to elucidate the role of different conditions in the processes. The effects of UV wavelength, the dosage of Fe(2+), the dosage of oxone, initial target compound concentration, solution pH and anions on the degradation efficiency were studied. In general the FOU is best performed among the processes...
October 14, 2016: Chemosphere
https://www.readbyqxmd.com/read/27737617/improving-thermal-hysteresis-activity-of-antifreeze-protein-from-recombinant-pichia-pastoris-by-removal-of-n-glycosylation
#9
Eun Jae Kim, Jun Hyuck Lee, Sung Gu Lee, Se Jong Han
To survive in a sub-zero environment, polar organisms produce ice-binding proteins (IBPs). These IBPs prevent the formation of large intracellular ice crystals, which may be fatal to the organism. Recently, a recombinant FfIBP (an IBP from Flavobacterium frigoris PS1) was cloned and produced in Pichia pastoris using fed-batch fermentation with methanol feeding. In this study, we demonstrate that FfIBP produced by P. pastoris has a glycosylation site, which diminishes the thermal hysteresis (TH) activity of FfIBP...
October 13, 2016: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/27733482/high-throughput-hplc-ms-ms-method-for-quantification-of-ibuprofen-enantiomers-in-human-plasma-focus-on-investigation-of-metabolite-interference
#10
Natalija Nakov, Liljana Bogdanovska, Jelena Acevska, Jasmina Tonic-Ribarska, Rumenka Petkovska, Aneta Dimitrovska, Lilia Kasabova, Dobrin Svinarov
In this research, as a part of the development of fast and reliable HPLC-MS/MS method for quantification of ibuprofen (IBP) enantiomers in human plasma, the possibility of IBP acylglucoronide (IBP-Glu) back-conversion was assessed. This involved investigation of in source and in vitro back-conversion. The separation of IBP enantiomers, its metabolite and rac-IBP-d3 (internal standard), was achieved within 6 min using Chiracel OJ-RH chromatographic column (150 × 2.1 mm, 5 μm). The followed selected reaction monitoring transitions for IBP-Glu (m/z 381...
November 2016: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/27720848/evaluation-of-anti-hypertensive-activity-of-ulmus-wallichiana-extract-and-fraction-in-shr-doca-salt-and-l-name-induced-hypertensive-rats
#11
Anees A Syed, Shibani Lahiri, Divya Mohan, Guru R Valicherla, Anand P Gupta, Mohammed Riyazuddin, Sudhir Kumar, Rakesh Maurya, Kashif Hanif, Jiaur R Gayen
ETHNOPHARMACOLOGICAL RELEVANCE: Ulmus wallichiana Planchon (Himalayan Elm), a traditional medicinal plant, used in fracture healingin folk tradition of Uttarakhand, Himalaya, India. It is also used as diuretic. U. rhynchophylla, native to China, known as Gou Teng in Chinese medicine, is used for hypertension (WHO). U. macrocarpa has antihypertensive and vasorelaxant activity. However, no detailed studies related to hypertension have been reported previously, so we have explored the antihypertensive activity of U...
October 5, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27701000/effect-of-halide-ions-on-the-photodegradation-of-ibuprofen-in-aqueous-environments
#12
Fuhua Li, Qingqing Kong, Ping Chen, Min Chen, Guoguang Liu, Wenying Lv, Kun Yao
Typically contained within ambient surface waters and certain industrial wastewaters, are plentiful halide ions, which possess varying degrees of photosensitivity. The effects of halide ions on the photodegradation of ibuprofen (IBP) were investigated under UV irradiation using a 500 W mercury lamp as a light source. Studies of the mechanism of halide ions were inclusive of both their light shielding effects and quenching experiments. The results indicated that chloride ion has a slight inhibition against IBP photodegradation under neutral condition, and significant inhibition is observed with bromide ions and iodide ions...
January 2017: Chemosphere
https://www.readbyqxmd.com/read/27684325/laboratory-production-of-biofuels-and-biochemicals-from-a-rapeseed-oil-through-catalytic-cracking-conversion
#13
Siauw H Ng, Yu Shi, Nicole E Heshka, Yi Zhang, Edward Little
The work is based on a reported study which investigates the processability of canola oil (bio-feed) in the presence of bitumen-derived heavy gas oil (HGO) for production of transportation fuels through a fluid catalytic cracking (FCC) route. Cracking experiments are performed with a fully automated reaction unit at a fixed weight hourly space velocity (WHSV) of 8 hr(-1), 490-530 °C, and catalyst/oil ratios of 4-12 g/g. When a feed is in contact with catalyst in the fluid-bed reactor, cracking takes place generating gaseous, liquid, and solid products...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27665746/the-motility-of-esophageal-sphincters-during-liquid-and-solid-bolus-swallows-a-multicenter-normative-value-study-of-high-resolution-manometry-in-china
#14
X-L Xiang, A Wang, L Tu, M-Y Ke, Y-S Yang, B Jiang, L Lin, N Dai, S-S Zhang, L Tao, H Xu, X-M Liang, X-C Fang, Z-W Xia, X Wang, J-N Wu, M-F Wang, H-J Zhang, Y-F Fang, C Shen, J Wang, L-H Peng, W-Y Li, Z-F Wang, K Wang, N Liu, X-H Hou
BACKGROUND: It is gradually accepted that solid bolus swallow needs to be added to the procedure of manometry. The motility differences in the upper esophageal sphincter (UES) and lower esophageal sphincter (LES) were not well described. Sierra Scientific Instruments solid-state high-resolution manometry (HRM) system, the most popular HRM system in China, lacks the Chinese normative values for both liquid and solid bolus swallow parameters. METHODS: The esophageal HRM data of 88 healthy volunteers were analyzed...
September 25, 2016: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/27663584/recognizing-the-elbow-prosthesis-on-conventional-radiographs
#15
Kamilcan Oflazoglu, Nienke Koenrades, Matthijs P Somford, Michel P J van den Bekerom
The objective of this study was to make an overview that can be useful in determining which type and brand of prosthesis a patient has when visiting the emergency department or outpatient clinic with a periprosthetic fracture, dislocation, or implant failure. The commonly used prostheses in Europe are opted for this list. The radiographs used for this list are obtained either from the company or from our own patients. This list contains the Coonrad/Morrey total elbow prosthesis, the Nexel total elbow prosthesis, the GSB III Elbow Prosthesis, the iBP Total Elbow System, the Discovery Elbow System, the NESimplavit Elbow System, the Latitude Elbow prosthesis, the Solar Elbow, and the Souter-Strathclyde total elbow...
September 23, 2016: Strategies in Trauma and Limb Reconstruction
https://www.readbyqxmd.com/read/27640060/fast-photocatalytic-degradation-of-pharmaceutical-micropollutants-and-ecotoxicological-effects
#16
Isabella Natali Sora, Davide Fumagalli
Aqueous solutions of ciprofloxacin (CP) and ibuprofen (IBP) in the presence of LaFeO3 photocatalyst, of H2O2, and of both LaFeO3 and H2O2 were irradiated under visible light. The degradation rate in the presence of both LaFeO3 and H2O2 after 5 h irradiation was more than 90 % for CP and 40 % for IBP, much higher than that with only H2O2 under visible light. For the sake of comparison, the experiments were also carried out in the dark, and both CP and IBP were not significantly converted. The degradation rate was enhanced by the simultaneous presence of small concentration of LaFeO3 (130 mg L(-1)) and H2O2 (0...
September 17, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27624889/targeting-protein-geranylgeranylation-slows-tumor-development-in-a-murine-model-of-prostate-cancer-metastasis
#17
Jacqueline E Reilly, Jeffrey D Neighbors, Raymond J Hohl
The isoprenoid biosynthetic pathway (IBP) plays a critical role in providing substrates and enzymes necessary for the post-translational modification and thus activation of a number of proteins involved in prostate cancer metastasis. Previous work by our lab found novel compound disodium [(6Z,11E,15E)-9-[bis(sodiooxy)phosphoryl]-17-hydroxy-2,6,12,16-tetramethyheptadeca-2,6,11,15-tetraen-9-yl]phosphonate (GGOHBP), which inhibits the IBP enzyme geranylgeranyl diphosphate synthase (GGDPS), reduced protein geranylgeranylation without altering protein farnesylation...
September 13, 2016: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/27612834/unusual-anti-leukemia-activity-of-nanoformulated-naproxen-and-other-non-steroidal-anti-inflammatory-drugs
#18
Raj Kumar, Prem Felix Siril, Farideh Javid
The non-steroidal anti-inflammatory drugs (NSAIDs) are the most widely used pharmaceuticals worldwide. Interestingly, many of them have significant anticancer properties too. However, the poor water solubility of certain NSAIDs limits their application for cancer treatment. Nanosizing of such drugs can help to improve the solubility and this may result in enhanced anticancer activities too. Moreover, over dosages and the accompanying side effects of NSAIDs can be minimized by improving their solubility and bioavailability...
December 1, 2016: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/27605101/lncvar-a-database-of-genetic-variation-associated-with-long-non-coding-genes
#19
Xiaowei Chen, Yajing Hao, Ya Cui, Zhen Fan, Shunmin He, Jianjun Luo, Runsheng Chen
MOTIVATION: Long non-coding RNAs (lncRNAs) are essential in many molecular pathways, and are frequently associated with disease but the mechanisms of most lncRNAs have not yet been characterized. Genetic variations, including single nucleotide polymorphisms (SNPs) and structural variations, are widely distributed in the genome, including lncRNA gene regions. As the number of studies on lncRNAs grows rapidly, it is necessary to evaluate the effects of genetic variations on lncRNAs. RESULTS: Here, we present LncVar, a database of genetic variation associated with long non-coding genes in six species...
September 6, 2016: Bioinformatics
https://www.readbyqxmd.com/read/27603574/palindrome-analyser-a-new-web-based-server-for-predicting-and-evaluating-inverted-repeats-in-nucleotide-sequences
#20
Václav Brázda, Jan Kolomazník, Jiří Lýsek, Lucia Hároníková, Jan Coufal, Jiří Št'astný
DNA cruciform structures play an important role in the regulation of natural processes including gene replication and expression, as well as nucleosome structure and recombination. They have also been implicated in the evolution and development of diseases such as cancer and neurodegenerative disorders. Cruciform structures are formed by inverted repeats, and their stability is enhanced by DNA supercoiling and protein binding. They have received broad attention because of their important roles in biology. Computational approaches to study inverted repeats have allowed detailed analysis of genomes...
September 30, 2016: Biochemical and Biophysical Research Communications
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