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https://www.readbyqxmd.com/read/28109113/-effect-of-sodium-phenylbutyrate-on-the-sensitivity-of-pc3-dtx-resistant-prostate-cancer-cells-to-docetaxel
#1
Ya-Wen Xu, Shao-Bo Zheng, Bin-Sheng Chen, Yong Wen, Shan-Wen Zhu
OBJECTIVE: To investigate the effect of sodium phenylbutyrate (SPB) in modulating docetaxel resistance in human prostate cancer cells in vitro. METHODS: A PC3/docetaxel-resistant human prostate cancer cell line PC3/DTX was induced and examined for proliferation, viability, and cell inhibition rate in the presence of SPB. The concentration of concentration of docetaxel required to kill 50% of PC3/DTX cells incubated with 0, 1, 2, and 4 mmol/L SPB was determined using MTT assay...
2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28108407/purification-and-characterization-of-an-extracellular-ribonuclease-from-a-bacillus-sp-rns3-kx966412
#2
Gupta Shruti, Singh Sukhdev, Kanwar Shamsher Singh
Ribonucleases (RNases) catalyze the degradation of ribonucleic acid (RNA) into smaller nucleotides. RNases display angiogenic, neurotoxic, antitumor and immunosuppressive properties. In the present study, an extracellular RNase was successfully purified to homogeneity from a Bacillus sp. RNS3 (KX966412) by salting out at 0-50% ammonium sulphate saturation followed by the gel permeation (Sephadex G-100) chromatography. The multistep purification resulted in 10.4 fold purification of RNase with a yield of 3.12%...
January 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28108289/negative-feedback-regulation-between-microrna-let-7g-and-lox-1-mediated-hypoxia-induced-pasmcs-proliferation
#3
Wei-Fang Zhang, Tian-Tian Zhu, You-Wen Xiong, Ai-Zhen Xiong, Xiao-Yue Ge, Chang-Ping Hu, Zheng Zhang
BACKGROUND: Pulmonary hypertension (PH) is a proliferative disorder associated with enhanced proliferation and suppressed apoptosis of pulmonary artery smooth muscle cells (PASMCs). Our lately study demonstrated that let-7g inhibited hypoxia-induced proliferation of PASMCs via repressed c-myc-Bmi-1-p16 signaling pathway. However, the upstream of let-7g has not yet been fully defined. Previous studies have shown that LOX-1, a target of let-7g, could also regulate the expression of let-7g in human aortic endothelial cells...
January 17, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28107962/a-label-free-electrochemical-immunosensor-based-on-a-new-polymer-containing-aldehyde-and-ferrocene-groups
#4
Xiangyang Zhang, Youming Shen, Youyu Zhang, Guangyu Shen, Haiyan Xiang, Xiaofeng Long
In this work, a novel and convenient strategy for synthesis of a well-defined polymer containing both aldehyde and ferrocene functional groups by grafting 4-hydroxybenzaldehyde and ferrocenecarboxylhydrazide onto linear poly (vinylbenzyl chloride) was developed. The designed polymer film (polymer CAF) could be used as a new support matrix for fabrication of a label-free electrochemical immunosensor with high sensitivity, good stability and repeatability. With human IgG as an analyte model, the proposed electrochemical immunosensor showed a linear concentration range from 0...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107921/highly-selective-enrichment-of-phosphopeptides-using-zr-4-immobilized-titania-nanoparticles
#5
Junyong Dai, Miaode Wang, Hailong Liu
It is urgent to selectively enrich trace phosphorylated proteins or peptides from complex biological sample for mass spectrometry (MS)-based phosphoproteomics. In this study, a novel immobilized metal affinity chromatography (IMAC) material with DOTA(1,4,7,10-tetraazacyclododecane N, N', N'', N'''-tetra-acetic acid) coated on the surface of TiO2 nanomaterials and functionalized with the metal ion Zr(4+)(TiO2@DOTA-Zr) has been synthesized successfully to improve the enrichment specificity and sensitivity for phosphopeptides...
March 1, 2017: Talanta
https://www.readbyqxmd.com/read/28107698/the-natural-flavonoid-apigenin-sensitizes-human-cd44-prostate-cancer-stem-cells-to-cisplatin-therapy
#6
Suat Erdogan, Kader Turkekul, Rıza Serttas, Zeynep Erdogan
Prostate cancer (PCa) is the second most common type of cancer and the fifth leading cause of cancer-related death among men. Development of chemoresistance, tumor relapse and metastasis remain major barriers to effective treatment and all been identified to be associated with cancer stem cells (CSCs). Natural flavonoids such as apigenin have been shown to have the ability to improve the therapeutic efficacy of common chemotherapy agents through CSCs sensitization. Thus, the aim of this study was to evaluate the combination of apigenin with cisplatin on CD44(+) PCa stem cell growth and migration...
January 17, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28107288/effects-of-infant-formula-with-human-milk-oligosaccharides-on-growth-and-morbidity-a-randomized-multicenter-trial
#7
Giuseppe Puccio, Philippe Alliet, Cinzia Cajozzo, Elke Janssens, Giovanni Corsello, Norbert Sprenger, Susan Wernimont, Delphine Egli, Laura Gosoniu, Philippe Steenhout
OBJECTIVES: We evaluated effects of infant formula supplemented with 2 human milk oligosaccharides (HMOs) on infant growth, tolerance and morbidity. METHODS: Healthy infants, 0-14 days old, were randomized to an intact-protein, cow's-milk-based infant formula (Control, n = 87) or the same formula with 1.0 g/L 2'fucosyllactose (2'FL) and 0.5 g/L lacto-N-neotetraose (LNnT) (Test, n = 88) from enrollment to 6 months; all infants received standard follow-up formula without HMOs from 6-12 months...
January 20, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28107182/the-nitrobenzoxadiazole-derivative-mc3181-blocks-melanoma-invasion-and-metastasis
#8
Anastasia De Luca, Debora Carpanese, Maria Cristina Rapanotti, Tara Mayte Suarez Viguria, Maria Antonietta Forgione, Dante Rotili, Chiara Fulci, Egidio Iorio, Luigi Quintieri, Sergio Chimenti, Luca Bianchi, Antonio Rosato, Anna Maria Caccuri
The novel nitrobenzoxadiazole (NBD) derivative MC3181 is endowed with remarkable therapeutic activity in mice bearing both sensitive and vemurafenib-resistant human melanoma xenografts. Here, we report that subtoxic concentrations of this compound significantly reduced invasiveness of BRAF-V600D mutated WM115 and WM266.4 melanoma cell lines derived from the primary lesion and related skin metastasis of the same patient, respectively. The strong antimetastatic activity of MC3181 was observed in both 2D monolayer cultures and 3D multicellular tumor spheroids, and confirmed in vivo by the significant decrease in the number of B16-F10 melanoma lung metastases in drug-treated mice...
January 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/28106824/identification-of-the-muc2-promoter-as-a-strong-promoter-for-intestinal-gene-expression-through-generation-of-transgenic-quail-expressing-gfp-in-gut-epithelial-cells
#9
Rachel M Woodfint, Paula R Chen, Jinsoo Ahn, Yeunsu Suh, Seongsoo Hwang, Sang Suk Lee, Kichoon Lee
Identification of tissue- and stage-specific gene promoters is valuable for delineating the functional roles of specific genes in genetically engineered animals. Here, through the comparison of gene expression in different tissues by analysis of a microarray database, the intestinal specificity of mucin 2 (MUC2) expression was identified in mice and humans, and further confirmed in chickens by RT-PCR (reverse transcription-PCR) analysis. An analysis of cis-acting elements in avian MUC2 gene promoters revealed conservation of binding sites, within a 2...
January 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28106783/extract-of-polygala-tenuifolia-alleviates-stress-exacerbated-atopy-like-skin-dermatitis-through-the-modulation-of-protein-kinase-a-and-p38-mitogen-activated-protein-kinase-signaling-pathway
#10
Bongjun Sur, Bombi Lee, Ye Seul Yoon, Pooreum Lim, Riwon Hong, Mijung Yeom, Hyang Sook Lee, Hijoon Park, Insop Shim, Hyejung Lee, Young Pyo Jang, Dae-Hyun Hahm
Atopic dermatitis (AD) and stress create a vicious cycle: stress exacerbates atopic symptoms, and atopic disease elicits stress and anxiety. Targeting multiple pathways including stress and allergic inflammation is, therefore, important for treating AD. In this study, we investigated the remedial value of Polygala tenuifolia Willd. (PTW) for treating immobilization (IMO) stress-exacerbated atopy-like skin dermatitis and its underlying mechanism. Trimellitic anhydride (TMA) was applied to dorsal skin for sensitization and subsequently both ears for eliciting T-cell-dependent contact hypersensitivity in mice, which underwent 2 h-IMO stress and PTW administration for the latter 6 and 9 days in the ear exposure period of TMA, respectively...
January 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28106292/23-hydroxytormentic-acid-protects-human-dermal-fibroblasts-by-attenuating-uva-induced-oxidative-stress
#11
Hae Jeong Youn, Ki Bbeum Kim, Hyo-Sun Han, In-Sook An, Kyu Joong Ahn
Ultraviolet A (UVA), one of the major components of sunlight, can penetrate the dermal layer of the skin and generate reactive oxygen species (ROS). It causes alterations in the dermal connective tissue and gene expression, inflammation, photoaging, and DNA damage. Therefore, the harmful effects of UVA and strategies to reduce it have been consistently investigated. 23-Hydroxytormentic acid (23-HTA) has been demonstrated to improve drug-induced nephrotoxicity and exhibit several free radical scavenging effects with other molecules...
January 20, 2017: Photodermatology, Photoimmunology & Photomedicine
https://www.readbyqxmd.com/read/28106273/the-immunoglobulin-superfamily-member-cd200r-identifies-cells-involved-in-type-2-immune-responses
#12
Lars H Blom, Britta C Martel, Lau F Larsen, Camilla V Hansen, Malene P Christensen, Nanna Juel-Berg, Thomas Litman, Lars K Poulsen
BACKGROUND: The pathology of allergic diseases involves type 2 immune cells, such as Th2, ILC2, and basophils exerting their effect by production of IL-4, IL-5, and IL-13. However, surface receptors that are specifically expressed on type 2 immune cells are less well documented. The aim of this investigation was to identify surface markers associated with type 2 inflammation. METHODS: Naïve human CD4(+) T-cells were short-term activated in the presence or absence of IL-4, and analysed for expression of >300 cell-surface proteins...
January 20, 2017: Allergy
https://www.readbyqxmd.com/read/28105221/aberrant-promoter-methylation-of-cancer-related-genes-in-human-breast-cancer
#13
Liang Wu, Ye Shen, Xianzhen Peng, Simin Zhang, Ming Wang, Guisheng Xu, Xianzhi Zheng, Jianming Wang, Cheng Lu
The clinical relevance of aberrant DNA methylation is being increasingly recognized in breast cancer. The present study aimed to evaluate the promoter methylation status of seven candidate genes and to explore their potential use as a biomarker for the diagnosis of breast cancer. A total of 70 Chinese patients with breast cancer were recruited, and matched with 20 patients with benign breast disease (BBD). Methylation-specific polymerase chain reaction was performed to measure the methylation status of selected genes...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28104696/systematic-characterization-of-human-testis-specific-actin-capping-protein-%C3%AE-3-as-a-possible-biomarker-for-male-infertility
#14
T Soda, Y Miyagawa, N Ueda, K Takezawa, H Okuda, S Fukuhara, K Fujita, H Kiuchi, M Uemura, Y Okamoto, A Tsujimura, H Tanaka, N Nonomura
STUDY QUESTION: Is actin capping protein (CP) β3 involved in human spermatogenesis and male infertility? SUMMARY ANSWER: Human CPβ3 (hCPβ3) is expressed in testis, changes its localization dynamically during spermatogenesis, and has some association with male infertility. WHAT IS KNOWN ALREADY: The testis-specific α subunit of CP (CPα3) was previously identified in human, and mutations in the cpα3 gene in mouse were shown to induce malformation of the sperm head and male infertility...
January 18, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28104354/profound-loss-of-esophageal-tissue-differentiation-in-eosinophilic-esophagitis
#15
M Rochman, J Travers, C E Miracle, M C Bedard, T Wen, N P Azouz, J M Caldwell, K Kc, J D Sherrill, B P Davis, J K Rymer, K M Kaufman, M E Rothenberg
BACKGROUND: A key question in the allergy field is to understand how tissue specific disease is manifested. Eosinophilic esophagitis (EoE) is an emerging tissue specific allergic disease whose pathogenesis remains unclear. OBJECTIVE: Herein, we tested the hypothesis that a defect in tissue specific esophageal genes is an integral part of EoE pathogenesis. METHODS: We interrogated the pattern of expression of esophagus-specific signature genes derived from the Human Protein Atlas in the EoE transcriptome and in EPC2 esophageal epithelial cells...
January 16, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28104027/-inhibition-of-g9a-attenuates-cell-proliferation-via-the-mitochondrial-apoptosis-pathway-in-lung-adenocarcinoma
#16
H J Wan, W Lyu, L Yu, Z Y Zhou, Y J Hu, J Hu
Objective: The aim of this study is to investigate the effect of G9a inhibitor BIX-01294 on attenuating cell proliferation in human lung adenocarcinoma A549 cell line and the underlying molecular mechanism. Methods: Treated with BIX-01294, the growth and proliferation of A549 cells were detected by MTT assay and colony formation assay, and its impact on cell apoptosis was analyzed using flow cytometry. By Western blot, we explored the alterations in the expression of apoptosis-related proteins and the G9a catalysate, H3K9me and H3K9me2...
January 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28103989/-experimental-research-on-the-effects-of-different-activators-on-the-formation-of-platelet-rich-gel-and-the-release-of-bioactive-substances-in-human-platelet-rich-plasma
#17
Y Yang, W Zhang, B Cheng
Objective: To explore the effects of calcium gluconate and thrombin on the formation of platelet-rich gel (PRG) and the release of bioactive substances in human platelet-rich plasma (PRP) and the clinical significance. Methods: Six healthy blood donors who met the inclusion criteria were recruited in our unit from May to August in 2016. Platelet samples of each donor were collected for preparation of PRP. (1) PRP in the volume of 10 mL was collected from each donor and divided into thrombin activation group (TA, added with 0...
January 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28103916/tim-1-defines-a-human-regulatory-b-cell-population-that-is-altered-in-frequency-and-function-in-systemic-sclerosis-patients
#18
Octavio Aravena, Ashley Ferrier, Madhvi Menon, Claudia Mauri, Juan Carlos Aguillón, Lilian Soto, Diego Catalán
BACKGROUND: Systemic sclerosis (SSc) is a systemic autoimmune disease characterized by excessive production of extracellular matrix by fibroblasts on skin and internal organs. Although Th2 cells have been involved in fibroblast stimulation, hyperactivated B cells may also play an important role. Regulatory B cells (Bregs) are cells capable of inhibiting inflammatory responses and controlling autoimmune diseases. Although many Breg populations have in common the ability to produce high amounts of IL-10, a unique surface marker defining most human Bregs is lacking...
January 19, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28103900/gde2-is-essential-for-neuronal-survival-in-the-postnatal-mammalian-spinal-cord
#19
Clinton Cave, Sungjin Park, Marianeli Rodriguez, Mai Nakamura, Ahmet Hoke, Mikhail Pletnikov, Shanthini Sockanathan
BACKGROUND: Glycerophosphodiester phosphodiesterase 2 (GDE2) is a six-transmembrane protein that cleaves glycosylphosphatidylinositol (GPI) anchors to regulate GPI-anchored protein activity at the cell surface. In the developing spinal cord, GDE2 utilizes its enzymatic function to regulate the production of specific classes of motor neurons and interneurons; however, GDE2's roles beyond embryonic neurogenesis have yet to be defined. METHOD: Using a panel of histological, immunohistochemical, electrophysiological, behavioral, and biochemistry techniques, we characterized the postnatal Gde2 (-/-) mouse for evidence of degenerative neuropathology...
January 19, 2017: Molecular Neurodegeneration
https://www.readbyqxmd.com/read/28103869/sinularin-induces-dna-damage-g2-m-phase-arrest-and-apoptosis-in-human-hepatocellular-carcinoma-cells
#20
Ting-Wen Chung, Shih-Chao Lin, Jui-Hsin Su, Yu-Kuo Chen, Chi-Chien Lin, Hong-Lin Chan
BACKGROUND: Sinularin isolated from the cultured soft coral Sinularia flexibilis has been reported to exert potent cytotoxic effects against particular types of cancer. This study was carried out to investigate the cytotoxic effects in sinularin-treated human hepatocellular carcinoma cells, HepG2, and to subsequently explore the underlying molecular mechanisms. METHODS: TheMTT (3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyl- tetrazolium bromide) method was used to evaluate the cytotoxicity of sinularin on HepG2 and Hep3B cell lines...
January 19, 2017: BMC Complementary and Alternative Medicine
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