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https://www.readbyqxmd.com/read/28654744/mimicking-enzyme-based-colorimetric-aptasensor-for-antibiotic-residue-detection-in-milk-combining-magnetic-loop-dna-probes-and-cha-assisted-target-recycling-amplification
#1
Qian Luan, Ning Gan, Yuting Cao, Tianhua Li
A mimicking enzyme-based colorimetric aptasensor was developed for the detection of kanamycin (KANA) in milk using magnetic loop-DNA-NMOF-Pt (m-L-DNA) probes and catalytic hairpin assembly (CHA)-assisted target recycling for signal amplification. The m-L-DNA probes were constructed via hybridization of hairpin DNA H1 (containing aptamer sequence) immobilized magnetic beads (m-H1) and signal DNA (sDNA, partial hybridization with H1) labeled nano Fe-MIL-88NH2-Pt (NMOF-Pt-sDNA). In the presence of KANA and complementary hairpin DNA H2, the m-L-DNA probes decomposed and formed an m-H1/KANA intermediate, which triggered the CHA reaction to form a stable duplex strand (m-H1-H2) while releasing KANA again for recycling...
June 27, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28654019/preventive-effect-of-cow-s-milk-fermented-with-lactobacillus-paracasei-cba-l74-on-common-infectious-diseases-in-children-a-multicenter-randomized-controlled-trial
#2
Giovanni Corsello, Maurizio Carta, Roberto Marinello, Marina Picca, Giulio De Marco, Maria Micillo, Dante Ferrara, Patrizia Vigneri, Gaetano Cecere, Pasqualina Ferri, Paola Roggero, Giorgio Bedogni, Fabio Mosca, Lorella Paparo, Rita Nocerino, Roberto Berni Canani
Background: Fermented foods have been proposed to prevent common infectious diseases (CIDs) in children attending day care or preschool. OBJECTIVES: To investigate the efficacy of dietary supplementation with cow's skim milk fermented with the probiotic Lactobacillus paracasei CBA L74 in reducing CIDs in children attending day care or preschool. Methods: Multicenter, randomized, double-blind, placebo-controlled trial on healthy children (aged 12-48 months) consuming daily 7 grams of cow's skim milk fermented with L...
June 27, 2017: Nutrients
https://www.readbyqxmd.com/read/28653977/exploitation-of-spr-to-investigate-the-importance-of-glycan-chains-in-the-interaction-between-lactoferrin-and-bacteria
#3
Noelle O'Riordan, Michelle Kilcoyne, Lokesh Joshi, Rita M Hickey
Bovine lactoferrin (LF) has been shown to prevent adhesion to and invasion of mammalian cell lines by pathogenic bacteria, with evidence for direct bacterial binding by the milk glycoprotein. However, the glycosylation pattern of LF changes over the lactation cycle. In this study, we aim to investigate the effect that this variation has on the milk glycoprotein's ability to interact with pathogens. Surface plasmon resonance technology was employed to compare the binding of LF from colostrum (early lactation) and mature milk (late lactation) to a panel of pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Cronobacter sakazakii, Streptococcus pneumoniae, Pseudomonas aeruginosa, Listeria monocytogenes and Salmonella typhimurium)...
June 27, 2017: Sensors
https://www.readbyqxmd.com/read/28653967/exercise-associated-hyponatremia-in-a-lactating-female
#4
Zach Bailowitz, Raymond Grams, David Teeple, Tamara Hew-Butler
A 37-year-old woman presented to the emergency department with severe headache, which quickly progressed to altered mental status and seizure activity in hospital. Her initial serum sodium concentration ([Na]) was 126 mmol/L. In the 24 hours before admission, she exercised vigorously for 120 minutes (interval training plus yoga) and also consumed more than 4 liters of fluid during that time to both stay hydrated and facilitate milk production because she was actively nursing 2 children. Her serum [Na] and altered mental status corrected slowly over the next 48 hours with furosemide, hypertonic saline, and fluid restriction...
July 2017: Clinical Journal of Sport Medicine: Official Journal of the Canadian Academy of Sport Medicine
https://www.readbyqxmd.com/read/28653875/mfg-e8-overexpression-promotes-colorectal-cancer-progression-via-akt-mmps-signalling
#5
Qiujie Zhao, Lin Xu, Xiaoyan Sun, Kai Zhang, Huimin Shen, Yanan Tian, Fengkai Sun, Yanqing Li
Several studies have revealed that MFG-E8 (milk fat globule-epidermal growth factor 8) is related to tumour development and progression. However, the relationship between MFG-E8 expression and metastasis in colorectal cancer patients and the role of MFG-E8 in colorectal cancer invasion and progression remain unknown. In this study, we performed immunohistochemistry and quantitative real-time polymerase chain reaction to assess MFG-E8 expression in colorectal cancer and adjacent non-cancerous tissues. Colorectal cancer RNAseq data from The Cancer Genome Atlas project were downloaded and MFG-E8 expression was analysed...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28653795/reduced-energy-density-of-close-up-diets-decrease-ruminal-ph-and-increase-concentration-of-volatile-fatty-acids-postpartum-in-holstein-cows
#6
Wenming Huang, Yujia Tian, Shengli Li, Zhaohai Wu, Zhijun Cao
The objective of this study was to determine the effect of reduced energy density of close-up diets on ruminal fermentation parameters in transition cows. Fourteen Holstein dry cows were blocked and assigned randomly to three groups fed a high energy density diet (HD, 1.62 Mcal of net energy for lactation (NEL )/kg dry matter (DM)), or a middle energy density diet (MD, 1.47 Mcal NEL /kg DM), or a low energy density diet (LD, 1.30 Mcal NEL /kg DM) prepartum, and were fed the same diet postpartum. The reduced energy density diets decreased the average dry matter intake (DMI) prepartum and tended to increase the DMI postpartum...
June 27, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28653388/factors-associated-with-colostrum-immunoglobulin-g-concentration-in-northern-victorian-dairy-cows
#7
A J Phipps, D S Beggs, A J Murray, P D Mansell, M F Pyman
OBJECTIVES: To determine the proportion of first-milking colostrum samples produced on four northern-Victorian dairy farms that meet industry standards in terms of immunoglobulin G (IgG) concentration and to identify risk factors that affect colostrum quality. METHODS: Colostrum IgG concentrations from 442 dairy cows on four farms were estimated using a Brix refractometer and risk factors for colostrum IgG concentration were determined using multivariable logistic regression...
July 2017: Australian Veterinary Journal
https://www.readbyqxmd.com/read/28653126/impact-of-metabolic-hormones-secreted-in-human-breast-milk-on-nutritional-programming-in-childhood-obesity
#8
REVIEW
Pilar Amellali Badillo-Suárez, Maricela Rodríguez-Cruz, Xóchitl Nieves-Morales
Obesity is the most common metabolic disease whose prevalence is increasing worldwide. This condition is considered a serious public health problem due to associated comorbidities such as diabetes mellitus and hypertension. Perinatal morbidity related to obesity does not end with birth; this continues affecting the mother/infant binomial and could negatively impact on metabolism during early infant nutrition. Nutrition in early stages of growth may be essential in the development of obesity in adulthood, supporting the concept of "nutritional programming"...
June 27, 2017: Journal of Mammary Gland Biology and Neoplasia
https://www.readbyqxmd.com/read/28652648/role-of-ph-in-the-recovery-of-bovine-milk-oligosaccharides-from-colostrum-whey-permeate-by-nanofiltration
#9
Joshua L Cohen, Daniela Barile, Yan Liu, Juliana M L N de Moura Bell
Milk oligosaccharides are associated with improved health outcomes in infants. Nanofiltration (NF) is used for isolation of bovine milk oligosaccharides (BMO). The study aim was to improve the recovery of BMO from lactose-hydrolyzed colostrum whey permeate. The retention factors of carbohydrates at various pH and transmembrane pressures were determined for a nanofiltration membrane, which was used at pilot scale to purify BMO. Carbohydrates were quantified by liquid chromatography and characterized using nano-LC-Chip-QToF mass spectrometry...
March 2017: International Dairy Journal
https://www.readbyqxmd.com/read/28652150/biochemical-characterization-of-a-novel-%C3%AE-galactosidase-from-paenibacillus-barengoltzii-suitable-for-lactose-hydrolysis-and-galactooligosaccharides-synthesis
#10
Yu Liu, Zhou Chen, Zhengqiang Jiang, Qiaojuan Yan, Shaoqing Yang
A β-galactosidase gene (PbBGal2A) was cloned from Paenibacillus barengoltzii and expressed in Escherichia coli. The in silico analysis of the deduced amino acid sequences revealed that PbBGal2A shared the highest identity of 40% with the characterized glycoside hydrolase (GH) family 2 β-galactosidase from Actinobacillus pleuropneumoniae. The recombinant β-galactosidase (PbBGal2A) was purified with a molecular mass of 124.2kDa on SDS-PAGE. The optimal pH and temperature of PbBGal2A were determined to be pH 7...
June 23, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28651696/importance-of-the-regiospecific-distribution-of-long-chain-saturated-fatty-acids-on-gut-comfort-fat-and-calcium-absorption-in-infants
#11
REVIEW
Valérie Petit, Laurence Sandoz, Clara L Garcia-Rodenas
Gastrointestinal tolerance and fat and calcium (Ca) absorption are different between breast-fed (BF) and formula-fed (FF) infants. Certain components and/or structural particularities in human milk (HM), can contribute to favorable outcomes in BF infants. In HM, the long-chain saturated fatty acid (LCSFA) palmitic acid has a different stereospecific distribution (sn-2 position) compared to most infant formula (IF) (primarily sn-1, 3 positions), which may contribute to unfavorable outcomes. Evidence suggests palmitic acid is important in the formation of stool FA-mineral (or FA-Ca) soaps, associated with harder stools in FF infants...
June 2017: Prostaglandins, Leukotrienes, and Essential Fatty Acids
https://www.readbyqxmd.com/read/28651692/long-chain-fatty-acids-and-related-pro-inflammatory-specialized-pro-resolving-lipid-mediators-and-their-intermediates-in-preterm-human-milk-during-the-first-month-of-lactation
#12
D T Robinson, H L Palac, V Baillif, E Van Goethem, M Dubourdeau, L Van Horn, C R Martin
This study aimed to measure longitudinal quantities of the long chain fatty acids, their biologically active terminal metabolites and related intermediates (also called oxylipins) in preterm human milk expressed during the first month of lactation. In a prospective cohort, breast milk was collected throughout the first month of lactation in 30 women who delivered preterm infants. Eighteen bioactive lipids and their intermediates were quantified via solid phase extraction and LC-MS/MS. Analysis by GC-FID quantified the fatty acid precursors...
June 2017: Prostaglandins, Leukotrienes, and Essential Fatty Acids
https://www.readbyqxmd.com/read/28651682/the-wuhan-twin-birth-cohort-wtbc
#13
Jinzhu Zhao, Shaoping Yang, Anna Peng, Zhengmin Qian, Hong Xian, Tianjiao Chen, Guanghui Dong, Yiming Zhang, Xijiang Hu, Zhong Chen, Jiangxia Cao, Xiaojie Song, Shunqing Xu, Tongzhang Zheng, Bin Zhang
The Wuhan Pre/Post-Natal Twin Birth Registry (WPTBR) is one of the largest twin birth registries with comprehensive medical information in China. It recruits women from the first trimester of pregnancy and their twins from birth. From January 2006 to May 2016, the total number of twins enrolled in WPTBR is 13,869 twin pairs (27,553 individuals). The WPTBR initiated the Wuhan Twin Birth Cohort (WTBC). The WTBC is a prospective cohort study carried out through incorporation of three samples. The first one comprises 6,920 twin pairs, and the second one, 6,949 twin pairs...
June 27, 2017: Twin Research and Human Genetics: the Official Journal of the International Society for Twin Studies
https://www.readbyqxmd.com/read/28651665/using-milk-leukocyte-differentials-for-diagnosis-of-subclinical-bovine-mastitis
#14
Juliano Leonel Gonçalves, Roberta L Lyman, Mitchell Hockett, Rudy Rodriguez, Marcos Veiga Dos Santos, Kevin L Anderson
This research study aimed to evaluate the use of the milk leukocyte differential (MLD) to: (a) identify quarter milks that are culture-positive; and (b) characterize the milk leukocyte responses to specific groups of pathogens causing subclinical mastitis. The MLD measures the absolute number and relative percentage of inflammatory cells in milk samples. Using the MLD in two dairy herds (170 and 172 lactating cows, respectively), we studied all lactating cows with a most recent monthly Dairy Herd Improvement Association somatic cell count (SCC) >200 × 103 cells/ml...
June 27, 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/28651664/the-effect-of-varying-proportion-and-chop-length-of-lucerne-silage-in-a-maize-silage-based-total-mixed-ration-on-diet-digestibility-and-milk-yield-in-dairy-cattle
#15
A L Thomson, D J Humphries, A K Jones, C K Reynolds
The objective was to assess the effects of inclusion rate and chop length of lucerne silage, when fed in a total mixed ration (TMR), on milk yield, dry matter (DM) intake (DMI) and digestion in dairy cows. Diets were formulated to contain a 50 : 50 ratio of forage : concentrate (DM basis) and to be isonitrogenous (170 g/kg CP). The forage portion of the offered diets was comprised of maize and lucerne silage in proportions (DM basis) of either 25 : 75 (high Lucerne (HL)) or 75 : 25 (low lucerne (LL)). Lucerne was harvested and conserved as silage at either a long (L) or short (S) chop length...
June 27, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28651630/maternal-inheritance-of-bifidobacterial-communities-and-bifidophages-in-infants-through-vertical-transmission
#16
Sabrina Duranti, Gabriele Andrea Lugli, Leonardo Mancabelli, Federica Armanini, Francesca Turroni, Kieran James, Pamela Ferretti, Valentina Gorfer, Chiara Ferrario, Christian Milani, Marta Mangifesta, Rosaria Anzalone, Moreno Zolfo, Alice Viappiani, Edoardo Pasolli, Ilaria Bariletti, Rosarita Canto, Rosanna Clementi, Marina Cologna, Tiziana Crifò, Giuseppina Cusumano, Sabina Fedi, Stefania Gottardi, Claudia Innamorati, Caterina Masè, Daniela Postai, Daniela Savoi, Massimo Soffiati, Saverio Tateo, Anna Pedrotti, Nicola Segata, Douwe van Sinderen, Marco Ventura
BACKGROUND: The correct establishment of the human gut microbiota represents a crucial development that commences at birth. Different hypotheses propose that the infant gut microbiota is derived from, among other sources, the mother's fecal/vaginal microbiota and human milk. RESULTS: The composition of bifidobacterial communities of 25 mother-infant pairs was investigated based on an internal transcribed spacer (ITS) approach, combined with cultivation-mediated and genomic analyses...
June 26, 2017: Microbiome
https://www.readbyqxmd.com/read/28651380/utilization-of-acid-pre-treated-coconut-dregs-as-a-substrate-for-production-of-detergent-compatible-lipase-by-bacillus-stratosphericus
#17
Nur Bainun Mohd Zin, Busyra Mohamad Yusof, Siti Nurbaya Oslan, Helmi Wasoh, Joo Shun Tan, Arbakariya B Ariff, Murni Halim
In recent years, many efforts have been directed to explore the methods to reduce the production costs of industrial lipase by improving the yield and the use of low-cost agricultural wastes. Coconut dregs, which is a lignocellulosic by-product from coconut oil and milk processing plants, is rich in cellulose (36%) and crude fat (9%). A newly isolated Bacillus stratosphericus has been demonstrated to perform cellulose hydrolysis on coconut dregs producing fermentable sugars. The highest extracellular lipase activity of 140 U/mL has been achieved in submerged fermentation with acid pre-treated coconut dregs...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28651240/indirect-spectrophotometric-determination-of-sulfadiazine-based-on-localized-surface-plasmon-resonance-peak-of-silver-nanoparticles-after-cloud-point-extraction
#18
Elahe Kazemi, Shayessteh Dadfarnia, Ali Mohammad Haji Shabani, Mohammad Reza Fattahi, Javad Khodaveisi
A novel, efficient, easy to use, environmentally friendly and cost-effective methodology is developed for the indirect spectrophotometric determination of sulfadiazine in different samples. The method is based on the micelle-mediated extraction of silver sulfadiazine and converting the silver content of the resultant surfactant-rich phase to the silver nanoparticles via generation of [Ag(NH3)2](+) followed by its chemical reduction using ascorbic acid. The changes in the amplitude of localized surface plasmon resonance peak of silver nanoparticles as a function of sulfadiazine concentration in the sample solution was monitored using fiber optic linear array spectrophotometry at 457nm...
June 17, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28651194/reference-intervals-for-hematological-and-biochemical-parameters-acute-phase-proteins-and-markers-of-oxidation-in-holstein-dairy-cows-around-3-and-30days-after-calving
#19
Pierangelo Moretti, Saverio Paltrinieri, Erminio Trevisi, Monica Probo, Annarita Ferrari, Andrea Minuti, Alessia Giordano
The establishment of specific reference intervals (RIs) is advisable when different metabolic conditions in the patient population are present or when existing RIs are not suitable. Holstein dairy cows, at the start of lactation, experience a negative energy balance and an altered immune function that can lead to different levels of blood parameters compared with cows at peak lactation. The aim of this study was the determination of RIs for Holstein cows at 3±1 and 30±3days in milk. To this aim, 145 cows, from 4 herds, were sampled...
June 20, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28651173/development-validation-and-clinical-application-of-a-method-for-the-simultaneous-quantification-of-lamivudine-emtricitabine-and-tenofovir-in-dried-blood-and-dried-breast-milk-spots-using-lc-ms-ms
#20
Catriona Waitt, Sujan Diliiy Penchala, Adeniyi Olagunju, Alieu Amara, Laura Else, Mohammed Lamorde, Saye Khoo
OBJECTIVES: To present the validation and clinical application of a LC-MS/MS method for the quantification of lamivudine (3TC), emtricitabine (FTC) and tenofovir (TFV) in dried blood spots (DBS) and dried breast milk spots (DBMS). METHODS: DBS and DBMS were prepared from 50 and 30μL of drug-spiked whole blood and human breast milk, respectively. Following extraction with acetonitrile and water, chromatographic separation utilised a Synergi polar column with a gradient mobile phase program consisting of 0...
June 17, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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