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code blue process analysis

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https://www.readbyqxmd.com/read/27871884/structural-characterization-of-recombinant-human-fibroblast-growth-factor-receptor-2b-kinase-domain-upon-interaction-with-omega-fatty-acids
#1
Masoumeh Moghadasi, Dariush Ilghari, Majid Sirati-Sabet, Abbas Amini, Hamideh Asghari, Nematollah Gheibi
The mutated recombinant kinase domain of human fibroblast growth factor receptor 2b (hFGFR2b) is overexpressed and purified, and its structural changes upon the interaction with three unsaturated fatty acids (UFAs), oleic, linoleic and α-linolenic are studied. This interaction is investigated to find out about the folding and unfolding effect of unsaturated fatty acids on the kinase domain structure of hFGFR2b. Recombinant pLEICS-01 vectors, containing the mutated coding region of hFGFR2b, are expressed in the standard Escherichia coli BL21 (DE3) host cells and purified by Ni(2+)-NTA affinity chromatography...
November 18, 2016: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/27369042/multidisciplinary-review-of-code-events-in-a-heart-center
#2
Angela C Blankenship, Richard P Fernandez, Brian F Joy, Julie C Miller, Aymen Naguib, Steven C Cassidy, Janet Simsic, Christina Phelps, Sheilah Harrison, Mark Galantowicz, Andrew R Yates
OBJECTIVE: To identify a cause for clinical deterioration, examine resuscitation efforts, and identify and correct system issues (thus improving outcomes) via a multidisciplinary code-review process soon after cardiopulmonary arrest. METHODS: Retrospective analysis of code events in a tertiary pediatric heart center from September 2010 to December 2013 and review of surgical-cardiac data from January 2010 to December 2013. RESULTS: A multidisciplinary team reviewed 47 code events, 16 of which (34%) were deemed potentially preventable...
July 2016: American Journal of Critical Care: An Official Publication, American Association of Critical-Care Nurses
https://www.readbyqxmd.com/read/26917098/acetylsalicylic-acid-induced-changes-in-the-chemical-coding-of-extrinsic-sensory-neurons-supplying-the-prepyloric-area-of-the-porcine-stomach
#3
L Rytel, J Calka
Acetylsalicylic acid is a popular drug that is commonly used to treat fever and inflammation, but which can also negativity affect the mucosal layer of the stomach, although knowledge concerning its influence on gastric innervation is very scarce. Thus, the aim of the present study was to study the influence of prolonged acetylsalicylic acid supplementation on the extrinsic primary sensory neurons supplying the porcine stomach prepyloric region. Fast Blue (FB) was injected into the above-mentioned region of the stomach...
March 23, 2016: Neuroscience Letters
https://www.readbyqxmd.com/read/26834575/high-resolution-mri-reveals-detailed-layer-structures-in-early-human-fetal-stages-in-vitro-study-with-histologic-correlation
#4
Rongpin Wang, Guangping Dai, Emi Takahashi
An understanding of normal fetal brain development is essential in detecting the early onset of brain disorders. It is challenging to obtain high-quality images that show detailed local anatomy in the early fetal stages because the fetal brain is very small with rapidly-changing complex structures related to brain development, including neurogenesis, neuronal migration, and axonal elongation. Previous magnetic resonance imaging (MRI) studies detected three layers throughout the fetal cerebral wall that showed differences in MR contrasts at 10 gestational weeks (GW), which is one of the earliest ages studied using MRI...
2015: Frontiers in Neuroanatomy
https://www.readbyqxmd.com/read/26749082/dual-energy-can-accurately-differentiate-uric-acid-containing-urinary-calculi-from-calcium-stones
#5
A Spek, F Strittmatter, A Graser, P Kufer, C Stief, M Staehler
PURPOSE: To retrospectively evaluate the accuracy of dual-energy CT (DECT) in the detection of the chemical composition of urinary calculi in correlation with infrared spectroscopic stone analysis. METHODS: We reviewed the CT scans of 255 patients who underwent DECT due to a clinical suspicion of urolithiasis. Out of this group, we included 64 patients with clinically symptomatic urolithiasis requiring stone removal. After surgical removal of the stone by ureterorenoscopy, chemical composition was analyzed with infrared spectroscopy...
September 2016: World Journal of Urology
https://www.readbyqxmd.com/read/26312735/a-new-method-of-detecting-changes-in-corneal-health-in-response-to-toxic-insults
#6
Mohammad Faisal Jamal Khan, Tapas C Nag, C Igathinathane, Uchechukwu L Osuagwu, Michele Rubini
The size and arrangement of stromal collagen fibrils (CFs) influence the optical properties of the cornea and hence its function. The spatial arrangement of the collagen is still questionable in relation to the diameter of collagen fibril. In the present study, we introduce a new parameter, edge-fibrillar distance (EFD) to measure how two collagen fibrils are spaced with respect to their closest edges and their spatial distribution through normalized standard deviation of EFD (NSDEFD) accessed through the application of two commercially available multipurpose solutions (MPS): ReNu and Hippia...
November 2015: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/25158132/identification-of-mytilus-edulis-genetic-regulators-during-early-development
#7
Sleiman Bassim, Arnaud Tanguy, Bertrand Genard, Dario Moraga, Rejean Tremblay
Understanding the mechanisms that enable growth and survival of an organism while driving it to the full range of its adaptation is fundamental to the issues of biodiversity and evolution, particularly regarding global climatic changes. Here we report the Illumina RNA-sequencing (RNA-seq) and de novo assembly of the blue mussel Mytilus edulis transcriptome during early development. This study is based on high-throughput data, which associates genome-wide differentially expressed transcript (DET) patterns with early activation of developmental processes...
November 1, 2014: Gene
https://www.readbyqxmd.com/read/24993493/institutional-resuscitation-protocols-do-they-affect-cardiopulmonary-resuscitation-outcomes-a-6-year-study-in-a-single-tertiary-care-centre
#8
Kanwalpreet Sodhi, Manender Kumar Singla, Anupam Shrivastava
PURPOSE: Despite advances in cardiopulmonary resuscitation and widespread life-support trainings, the outcomes of resuscitation are variable. There is a definitive need for organizational inputs to strengthen the resuscitation process. Our hospital authorities introduced certain changes at the organizational level in our in-house resuscitation protocol. We aimed to study the impact of these changes on the outcomes of resuscitation. METHODS: The hospital code blue committee decided to reformulate the resuscitation protocols and reframe the code blue team...
February 2015: Journal of Anesthesia
https://www.readbyqxmd.com/read/24899205/kaposi-s-sarcoma-associated-herpesvirus-k3-and-k5-ubiquitin-e3-ligases-have-stage-specific-immune-evasion-roles-during-lytic-replication
#9
Kevin Brulois, Zsolt Toth, Lai-Yee Wong, Pinghui Feng, Shou-Jiang Gao, Armin Ensser, Jae U Jung
UNLABELLED: The downregulation of immune synapse components such as major histocompatibility complex class I (MHC-I) and ICAM-1 is a common viral immune evasion strategy that protects infected cells from targeted elimination by cytolytic effector functions of the immune system. Kaposi's sarcoma-associated herpesvirus (KSHV) encodes two membrane-bound ubiquitin E3 ligases, called K3 and K5, which share the ability to induce internalization and degradation of MHC-I molecules. Although individual functions of K3 and K5 outside the viral genome are well characterized, their roles during the KSHV life cycle are still unclear...
August 2014: Journal of Virology
https://www.readbyqxmd.com/read/24591602/categorical-encoding-of-color-in-the-brain
#10
Chris M Bird, Samuel C Berens, Aidan J Horner, Anna Franklin
The areas of the brain that encode color categorically have not yet been reliably identified. Here, we used functional MRI adaptation to identify neuronal populations that represent color categories irrespective of metric differences in color. Two colors were successively presented within a block of trials. The two colors were either from the same or different categories (e.g., "blue 1 and blue 2" or "blue 1 and green 1"), and the size of the hue difference was varied. Participants performed a target detection task unrelated to the difference in color...
March 25, 2014: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/24074431/automated-analysis-of-electronic-medical-record-data-reflects-the-pathophysiology-of-operative-complications
#11
Joseph J Tepas, Joan M Rimar, Allen L Hsiao, Michael S Nussbaum
PURPOSE: We hypothesized that a novel algorithm that uses data from the electronic medical record (EMR) from multiple clinical and biometric sources could provide early warning of organ dysfunction in patients with high risk for postoperative complications and sepsis. Operative patients undergoing colorectal procedures were evaluated. METHODS: The Rothman Index (RI) is a predictive model based on heuristic equations derived from 26 variables related to inpatient care...
October 2013: Surgery
https://www.readbyqxmd.com/read/23887809/the-suggestible-brain-posthypnotic-effects-on-value-based-decision-making
#12
Vera U Ludwig, Christine Stelzel, Harald Krutiak, Amadeus Magrabi, Rosa Steimke, Lena M Paschke, Norbert Kathmann, Henrik Walter
Hypnosis can affect perception, motor function and memory. However, so far no study using neuroimaging has investigated whether hypnosis can influence reward processing and decision-making. Here, we assessed whether posthypnotic suggestions can diminish the attractiveness of unhealthy food and whether this is more effective than diminishing attractiveness by one's own effort via autosuggestion. In total, 16 participants were hypnotized and 16 others were instructed to associate a color cue (blue or green) with disgust regarding specific snacks (sweet or salty)...
September 2014: Social Cognitive and Affective Neuroscience
https://www.readbyqxmd.com/read/23588419/roles-for-blue-light-jasmonate-and-nitric-oxide-in-the-regulation-of-dormancy-and-germination-in-wheat-grain-triticum-aestivum-l
#13
John V Jacobsen, Jose M Barrero, Trijntje Hughes, Magdalena Julkowska, Jennifer M Taylor, Qian Xu, Frank Gubler
Abscisic acid (ABA) plays a central role in seed dormancy and transcriptional regulation of genes coding for ABA biosynthetic and degradation enzymes is responsible for control of ABA content. However, little is known about signalling both before and after ABA regulation, in particular, how environmental signals are perceived and transduced. We are interested in these processes in cereal grains, particularly in relation to the development of strategies for controlling pre-harvest sprouting in barley and wheat...
July 2013: Planta
https://www.readbyqxmd.com/read/23521393/expanding-the-spectral-palette-of-fluorescent-proteins-for-the-green-microalga-chlamydomonas-reinhardtii
#14
COMPARATIVE STUDY
Beth A Rasala, Daniel J Barrera, Jenny Ng, Thomas M Plucinak, Julian N Rosenberg, Donald P Weeks, George A Oyler, Todd C Peterson, Farzad Haerizadeh, Stephen P Mayfield
Fluorescent proteins (FPs) have become essential tools for a growing number of fields in biology. However, such tools have not been widely adopted for use in microalgal research. The aim of this study was to express and compare six FPs (blue mTagBFP, cyan mCerulean, green CrGFP, yellow Venus, orange tdTomato and red mCherry) in the popular model microalga Chlamydomonas reinhardtii. To circumvent the transgene silencing that often occurs in C. reinhardtii, the FPs were expressed from the nuclear genome as transcriptional fusions with the sh-ble antibiotic resistance gene, with the foot and mouth disease virus 2A self-cleaving sequence placed between the coding sequences...
May 2013: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/23139743/approaches-to-brain-stress-testing-bold-magnetic-resonance-imaging-with-computer-controlled-delivery-of-carbon-dioxide
#15
W Alan C Mutch, Daniel M Mandell, Joseph A Fisher, David J Mikulis, Adrian P Crawley, Olivia Pucci, James Duffin
BACKGROUND: An impaired vascular response in the brain regionally may indicate reduced vascular reserve and vulnerability to ischemic injury. Changing the carbon dioxide (CO(2)) tension in arterial blood is commonly used as a cerebral vasoactive stimulus to assess the cerebral vascular response, changing cerebral blood flow (CBF) by up to 5-11 percent/mmHg in normal adults. Here we describe two approaches to generating the CO(2) challenge using a computer-controlled gas blender to administer: i) a square wave change in CO(2) and, ii) a ramp stimulus, consisting of a continuously graded change in CO(2) over a range...
2012: PloS One
https://www.readbyqxmd.com/read/22744775/number-generation-bias-after-action-observation
#16
Arnaud Badets, Cédric A Bouquet, François Ric, Mauro Pesenti
Recent studies have demonstrated that conceptual and abstract knowledge could rely on and could be influenced by sensory-motor processing of usual goal-directed actions. In line with this, interactions have been reported between number magnitude and finger grip with, for example, small-magnitude numbers priming grip closure and large-magnitude numbers priming grip aperture. Here, we assessed whether observing a closing or opening grip was able to influence the magnitude of the numbers produced in a random number generation task, and we tested whether this effect was specific to biological hand actions by using non-biological fake hands with the same closure or aperture amplitude...
August 2012: Experimental Brain Research. Experimentelle Hirnforschung. Expérimentation Cérébrale
https://www.readbyqxmd.com/read/22502564/performance-of-laser-fluorescence-devices-and-visual-examination-for-the-detection-of-occlusal-caries-in-permanent-molars
#17
Peter Rechmann, Daniel Charland, Beate M T Rechmann, John D B Featherstone
The aim of this study was to evaluate the diagnostic capabilities of a laser fluorescence tool DIAGNOdent (KaVo, Biberach, Germany) and two light-emitting diode fluorescence tools-Spectra Caries Detection Aid (AIR TECHNIQUES, Melville, NY), and SOPROLIFE light-induced fluorescence evaluator in daylight and blue florescence mode (SOPRO, ACTEON Group, La Ciotat, France)-in comparison to the caries detection and assessment system (ICDAS-II) in detection of caries lesions. In 100 subjects (age 23.4±10.6 years), 433 posterior permanent unrestored teeth were examined...
March 2012: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/22496766/effects-of-the-cryptochrome-cryb-from-rhodobacter-sphaeroides-on-global-gene-expression-in-the-dark-or-blue-light-or-in-the-presence-of-singlet-oxygen
#18
Sebastian Frühwirth, Kristin Teich, Gabriele Klug
Several regulators are controlling the formation of the photosynthetic apparatus in the facultatively photosynthetic bacterium Rhodobacter sphaeroides. Among the proteins affecting photosynthesis gene expression is the blue light photoreceptor cryptochrome CryB. This study addresses the effect of CryB on global gene expression. The data reveal that CryB does not only influence photosynthesis gene expression but also genes for the non-photosynthetic energy metabolism like citric acid cycle and oxidative phosphorylation...
2012: PloS One
https://www.readbyqxmd.com/read/22353560/a-mutation-of-osotp-51-leads-to-impairment-of-photosystem-i-complex-assembly-and-serious-photo-damage-in-rice
#19
Jian-Wei Ye, Zi-Ying Gong, Chun-Guang Chen, Hua-Ling Mi, Gen-Yun Chen
Gene expression in chloroplasts is regulated by many nuclear-encoded proteins. In this study, we isolated a rice (Oryza sativa subsp. japonica) mutant osotp51 with significant reduction in photosystem I (PSI). The osotp51 is extremely sensitive to light and accumulates a higher level of reactive oxygen species. Its leaves are almost albino when grown at 40 μmol photons/m(2) per s. However, grown at 4 μmol photons/m(2) per s, osotp51 has a similar phenotype to the wild-type. 77K chlorophyll fluorescence analysis showed a blue shift in the highest peak emission from PSI in osotp51...
February 2012: Journal of Integrative Plant Biology
https://www.readbyqxmd.com/read/22273548/impact-of-parental-catastrophizing-and-contextual-threat-on-parents-emotional-and-behavioral-responses-to-their-child-s-pain
#20
Line Caes, Tine Vervoort, Zina Trost, Liesbet Goubert
Limited research has addressed processes underlying parents' empathic responses to their child's pain. The present study investigated the effects of parental catastrophizing, threatening information about the child's pain, and child pain expression upon parental emotional and behavioral responses to their child's pain. A total of 56 school children participated in a heat pain task consisting of 48 trials while being observed by 1 of their parents. Trials were preceded by a blue or yellow circle, signaling possible pain stimulation (i...
March 2012: Pain
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