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Heavy metal screen tests

Dorota Korsak, Magdalena Szuplewska
A total of 127 Listeria isolates from food and food processing environments, including 75 L. innocua, 49 L. welshimeri, 2 L. seeligeri and 1L. grayi were tested for susceptibility to eight antimicrobials, benzalkonium chloride (BC), cadmium and arsenic. The isolates were also screened for the presence of extrachromosomal genetic elements - plasmids, and their restriction pattern types were determined. All strains were susceptible to ampicillin, ciprofloxacin, erythromycin, gentamicin, rifampicin, trimethoprim and vancomycin...
August 26, 2016: International Journal of Food Microbiology
Jianfeng Zhang, Hongyan Hou, Guang Chen, Shusheng Wang, Jiejing Zhang
The straw can be degraded efficiently into humus by powerful enzymes from microorganisms, resulting in the accelerated circulation of N,P,K and other effective elements in ecological system. We isolated a strain through screening the straw degradation strains from natural humic straw in the low temperature area in northeast of china, which can produce cellulase efficiently. The strain was identified as Pseudomonas mendocina by using morphological, physiological, biochemical test, and molecular biological test, with the functional clarification on producing cellulase for Pseudomonas mendocina for the first time...
September 2, 2016: Bioengineered
Barbara Leśniewska, Marta Krymska, Ewelina Świerad, Józefa Wiater, Beata Godlewska-Żyłkiewicz
A fast ultrasound-assisted sequential extraction (UASE) procedure for the determination of cadmium, lead and nickel fractions in soil was developed and fully validated. The working parameters of an ultrasound probe were optimized by comparing the content of metals in soil extracts obtained by the UASE procedure with that obtained by the conventional (with the aid of a vertical rotor) modified Community Bureau of Reference (BCR) procedure. The content of metals in soil fractions was determined by electrothermal atomic absorption spectrometry...
September 27, 2016: Environmental Science and Pollution Research International
Sang-Yong Eom, Ji-Ae Lim, Yong-Dae Kim, Byung-Sun Choi, Myung Sil Hwang, Jung-Duck Park, Heon Kim, Ho-Jang Kwon
This study was performed to select single nucleotide polymorphisms (SNPs) related to the body burden of heavy metals in Koreans, to provide Korean allele frequencies of selected SNPs, and to assess the difference in allele frequencies with other ethnicities. The candidate-gene approach method and genome-wide association screening were used to select SNPs related to the body burden of heavy metals. Genotyping analysis of the final 192 SNPs selected was performed on 1,483 subjects using the VeraCode Goldengate assay...
July 2016: Toxicological Research
Yan He, Jian Xu, Changsheng Guo, Jiapei Lv, Yuan Zhang, Wei Meng
Contaminants accumulated in sediments may directly harm benthic organisms, however, the specific contaminants responsible for adverse effects have been poorly described. In this study, a bioassay-directed analysis combined with toxicity tests and chemistry analysis was conducted to determine the compounds eliciting the greatest toxicological effect in the sediments in Liaohe River, northeast China. A total of 24 sediment samples were examined to determine their acute toxicity to midge Chironomus tentans (C...
July 1, 2016: Environmental Pollution
Dieter Melchart, Stefan Hager, Jingzhang Dai, Wolfgang Weidenhammer
BACKGROUND: The use of drugs derived from plants is a cornerstone of Traditional Chinese Medicine (TCM). Yet, too little is known about risk and safety of Chinese medicinal drugs (CMD). Therefore, the TCM hospital Bad Kötzting has developed a quality control and complication screening programme in order to ensure a safe administration of TCM drugs to their patients. METHODS: All Chinese medicinal drugs delivered to the hospital between September 1, 2012 and December 31, 2013 entered the quality control program and were screened for microbial contamination, aflatoxin, pesticides and heavy metals...
2016: Forschende Komplementärmedizin, Research in Complementary Medicine
Mohammad Amin Rezvanfar, Mahshid Hodjat, Mohammad Abdollahi
Developmental toxicology is an important area of novel toxicology. In recent years, there have been big concerns toward the increasing exposure to pharmaceutical agents, food additives, pesticides, occupational toxicants, and environmental pollutants, as well as their possible association with all aspects of male or female-mediated transient or permanent defects in progeny. Therefore, it is of great importance to look for new predictive models to evaluate environmental toxicants before they can harm the human health and embryo development...
August 1, 2016: Life Sciences
Bilal Hussain, Tayyaba Sultana, Salma Sultana, Shahid Mahboob, K A Al-Ghanim, Shahid Nadeem
A large number of methods have been applied to evaluate genotoxic damage in different aquatic species. Comet assay, as a method for detecting DNA alterations, and micronucleus test, as an index of chromosomal damage are the most widely used and authentic methods in laboratory and field studies. The primary objective of the study was to evaluate the genotoxic effects of heavy metals generated by toxic industrial effluents and various kinds of pollutants from urban and agricultural areas and domestic waste on Catla catla and Cirrhinus mrigala due to water pollution in the Chenab River, Pakistan...
July 2016: Environmental Science and Pollution Research International
Hidekazu Arai, Katsuo Nakamura, Yasuko Yoshida, Kei Tawarada
Cadmium is a highly toxic heavy metal for humans. In 2006, the Codex Alimentarius Commission set the international standard value of Cd contained in polished rice at 0.4 mg kg(-1), thus requiring the monitoring of Cd in food. We developed two types of Cd immunochromatographic kit by using anti-(Cd-EDTA) antibody. One is the quantitative Cd immunochromatographic device which can precisely measure Cd concentrations. The other is the Cd screening strip that enables visible evaluation of Cd exceeding 0.003 mg L(-1)...
September 2016: Analytical and Bioanalytical Chemistry
Guojian Liao, Qianhua Wu, Renwei Feng, Junkang Guo, Ruigang Wang, Yingming Xu, Yongzhen Ding, Zhilian Fan, Liangyu Mo
Paddy soils in many regions of China have been seriously polluted by multiple heavy metals or metalloids, such as arsenic (As), cadmium (Cd) and lead (Pb). In order to ensure the safety of food and take full advantage of the limited farmland resources of China, exploring an effective technology to repair contaminated soils is urgent and necessary. In this study, three technologies were employed, including variety screening, water management and foliage dressing, to assess their abilities to reduce the accumulation of Cd and As in the grains of different rice varieties, and meanwhile monitor the related yields...
April 1, 2016: Journal of Environmental Management
Salvador Moncho, Jochen Autschbach
A benchmark study for relativistic density functional calculations of NMR spin-spin coupling constants has been performed. The test set contained 47 complexes with heavy metal atoms (W, Pt, Hg, Tl, Pb) with a total of 88 coupling constants involving one or two heavy metal atoms. One-, two-, three-, and four-bond spin-spin couplings have been computed at different levels of theory (nonhybrid vs hybrid DFT, scalar vs two-component relativistic). The computational model was based on geometries fully optimized at the BP/TZP scalar relativistic zeroth-order regular approximation (ZORA) and the conductor-like screening model (COSMO) to include solvent effects...
January 12, 2010: Journal of Chemical Theory and Computation
Bao Wang, Jian-xin Yu, Biao Huang, Wen-you Hu, Qing Chang
Portable X-ray fluorescence (PXRF) spectrometer as a new type of equipment for quick test has a prominent prospect, but there are also shortcomings of detection range and limition, therefore this paper studied the suitability of PXRF spectrometer in monitoring soil environmental qualities of heavy metals included Cr, Ni, Cu, Zn, Pb, Cd, As and Hg, the aim of this paper is to screen elements which can be detected by this kind of instrument and evaluate the accuracy of test results. The research method is to test heavy metals contaminated soil samples by PXRF spectrometer, evaluate the accuracy of test results of PXRF compared with inductively coupled plasma mass(ICP-MS), then establish linear regression relationship between analysis results of PXRF and ICP-MS method...
June 2015: Guang Pu Xue Yu Guang Pu Fen Xi, Guang Pu
Bulent Icgen, Fadime Yilmaz
Due to their metal removal ability, bacterial biosorbents can be effectively used for the treatment of wastewaters containing heavy metals. Searching for bacterial biosorbents for hazardous heavy metals like cadmium is a pivotal for remediation efforts. The gene cadA, that mediates resistance to cadmium over an ATP-dependent efflux mechanism, provides a good target for the selection of potential cadmium biosorbents. For this reason, in this study, a 36-mer-oligonucleotide DNA probe based on the entire 3.5-kb BglII-XbaI fragment of cadA operon from staphylococcal plasmid pI258 was prepared by using Vector NTI Express software...
March 2016: Environmental Science and Pollution Research International
Asoka Pathiratne, Chamini K Hemachandra, Nimal De Silva
Efficacy of Allium cepa test system for screening cytotoxicity and genotoxicity of treated effluents originated from four types of industrial activities (two textile industries, three rubber based industries, two common treatment plants of industrial zones, and two water treatment plants) was assessed. Physico-chemical parameters including the heavy metal/metalloid levels of the effluents varied depending on the industry profile, but most of the measured parameters in the effluents were within the specified tolerance limits of Sri Lankan environmental regulations for discharge of industrial effluents into inland surface waters...
December 2015: Environmental Monitoring and Assessment
Elizabeth Silva, María Elsa Villarreal, Omayda Cárdenas, Carlos Armando Cristancho, Carmenza Murillo, Manuel Alberto Salgado, Gerardo Nava
INTRODUCTION: Significant alterations may be found in the water of Bogotá´s water supply system after its purification, specifically during its distribution and storage in home reservoirs, which makes it necessary to study the final quality of the domiciliary water consumed by users. OBJECTIVE: To conduct a preliminary study of toxic chemical substances in the water supplied by Bogotá´s water supply system in samples obtained from residential reservoirs and faucets...
August 2015: Biomédica: Revista del Instituto Nacional de Salud
Shimaa M Motawei, Hossam E Gouda
Heavy metals toxicity is a prevalent health problem particularly in developing countries. Mercury and cadmium are toxic elements that have no physiologic functions in human body. They should not be present in the human body by any concentration. Copper, on the other hand, is one of the elements that are essential for normal cell functions and a deficiency as well as an excess of which can cause adverse health effects. To test blood levels of mercury, cadmium, and copper in pregnant women in Dakahlia, Egypt...
June 2016: Biological Trace Element Research
Armaghan Shafaei, Kameh Esmailli, Elham Farsi, Abdalrahim F A Aisha, Amin Malik Shah Abul Majid, Zhari Ismail
BACKGROUND: Orthosiphon stamineus (OS) Benth is a medicinal plant and native in Southeast Asia. Pharmacological effects of OS are attributed to the presence of lipophilic flavones. However; lipophilic compounds suffer from poor aqueous solubility which limits the OS oral bioavailability and therapeutic applications. Therefore, OS was prepared in nano formulation form using liposomes from soybean phospholipids. The aim of the present study is to evaluate the in vitro genotoxicity and in vivo oral toxicity of nano liposomes of OS ethanolic extract (OS-EL)...
2015: BMC Complementary and Alternative Medicine
Jiabo Wang, Zhijie Ma, Ming Niu, Yun Zhu, Qingsheng Liang, Yanling Zhao, Jingyuan Song, Zhaofang Bai, Yaming Zhang, Ping Zhang, Na Li, Yakun Meng, Qi Li, Lushan Qin, Guangju Teng, Junling Cao, Baosen Li, Shilin Chen, Yonggang Li, Zhengsheng Zou, Honghao Zhou, Xiaohe Xiao
Herbal medicines have recently been recognized as the second most common cause of drug-induced liver injury (DILI) in the United States. However, reliable methods to identify the DILI causality of some herbs, such as Heshouwu (dried root of Polygonum multiflorum), remain lacking. In this study, a total of 12 307 inpatients with liver dysfunction and 147 literature-reported cases of Heshouwu DILI were screened. A general algorithm indicated that only 22.5% (9/40) and 30.6% (45/147) of all hospitalization and literature case reports, respectively, demonstrate the high probability of DILI causality of Heshouwu...
December 2015: Frontiers of Medicine
Marcus G K Cumberbatch, Angela Cox, Dawn Teare, James W F Catto
IMPORTANCE: Bladder cancer (BC) is a common disease. Despite manufacturing and legislative changes to workplace hygiene, many BCs still arise through occupational carcinogen exposure. OBJECTIVE: To profile contemporary risks of occupational BC. DATA SOURCES: A systematic review using PubMed, Medline, Embase, and Web of Science was performed in October 2012 (initial review) and May 2014 (final review) and was updated in June 2015. STUDY SELECTION: We identified 263 eligible articles...
December 2015: JAMA Oncology
S M El-Megharbel, R Z Hamza, M S Refat, M A Al-Omar, A M Naglah
Four new complexes of Hg (II), Pb (II), Sn (II) and Bi (III) with indomethacin drug ligand (IMC) were synthesized and characterized by using infrared, electronic, 1H-NMR spectral, thermogravimetric and conductivity measurements. The IMC was found to act as bidentate chelating agent. IMC complexes coordinate through the oxygen of the carboxyl group. The molar ratio chelation is 1:2 (M2+:IMC) with general formula [M (IMC) 2], nH2O for Hg (II), Pb(II) and Sn(II), but 1:3 for Bi(III) ions. Antibacterial screening of these heavy metal complexes against Escherichia coli (Gram-ve), Bacillus subtilis (Gram +ve) and anti-fungi (Asperagillus oryzae, Asperagillus niger, Asperagillus Flavus) were investigated...
July 2015: Journal of Biological Regulators and Homeostatic Agents
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