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Heavy metal screen tests

G Y Fu, J Gong, X X Leng
Objective: To establish the method of colloidal gold immunochrom atographic assay for detecting cadmium ions rapidly. Methods: The anti-cadmium ion monoclonal antibody-gold conjugate was labeled on the binding pad, cadmium ion hapten and goat anti-mouse IgG were coated on nitrocellulose membrane as the detection line (T line) and quality control line (C line) respectively. The sample pad, colloidal gold bonding pad, nitrocellulose membrane and absorption pad were orderly assembled on the PVC board to cut into a test paper strip...
March 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
Syed Shameer
Extracellular Polysaccharides (EPS) from both prokaryotes and eukaryotes have a great deal of research interest as they protect the producer from different stresses including antibiotics, ionic stress, desiccation and assist in bio-film formation, pathogenesis, adhesion, etc. In this study haloalkaliphilic Bacillus sp., known to cope with osmophilic stress, was selected and screened for EPS production. The EPS were isolated, partially purified and chemical characteristics were documented using liquid FT-IR followed by assessment of heavy metal biosorption (lead, copper and cadmium) using Atomic Absorption Spectroscopy (AAS)...
December 2016: 3 Biotech
Marta Staniszewska, Helena Boniecka
This article deals with the legal and practical recommendations for the management of dredged material in the riparian countries of the Baltic Sea. The recommendations are contained in three conventions: LC, 2000. London Convention (1972), Convention on the Protection of the Marine Environment of the Baltic Sea area (Helsinki Convention) (1992), the OSPAR Convention (1972). Different approaches to evaluating the contamination level of dredge spoils, used by the Baltic Sea riparian countries, have been characterized...
January 2017: Environmental Monitoring and Assessment
Louise Josefine Belmonte, Lisbeth M Ottosen, Gunvor Marie Kirkelund, Pernille Erland Jensen, Andreas Peter Vestbø
In the vulnerable Arctic environment, the impact of especially hazardous wastes can have severe consequences and the reduction and safe handling of these waste types are therefore an important issue. In this study, two groups of heavy metal containing particulate waste materials, municipal solid waste incineration (MSWI) fly and bottom ashes and mine tailings (i.e., residues from the mineral resource industry) from Greenland were screened in order to determine their suitability as secondary resources in clay-based brick production...
November 10, 2016: Environmental Science and Pollution Research International
Pavel Trtik, Manuel Morgano, Roman Bentz, Eberhard Lehmann
The recent developments in scientific complementary metal oxide semiconductor (sCMOS) detector technology allow for imaging of relevant processes with very high temporal resolution with practically negligible readout time. However, it is neutron intensity that limits the high temporal resolution neutron imaging. In order to partially overcome the neutron intensity problem for the high temporal resolution imaging, a parabolic neutron focussing guide was utilized in the test arrangement and placed upstream the detector in such a manner that the focal point of the guide was positioned slightly behind the scintillator screen...
2016: MethodsX
Dorota Korsak, Magdalena Szuplewska
A total of 127 Listeria isolates from food and food processing environments, including 75 L. innocua, 49 L. welshimeri, 2 L. seeligeri and 1L. grayi were tested for susceptibility to eight antimicrobials, benzalkonium chloride (BC), cadmium and arsenic. The isolates were also screened for the presence of extrachromosomal genetic elements - plasmids, and their restriction pattern types were determined. All strains were susceptible to ampicillin, ciprofloxacin, erythromycin, gentamicin, rifampicin, trimethoprim and vancomycin...
December 5, 2016: International Journal of Food Microbiology
Jianfeng Zhang, Hongyan Hou, Guang Chen, Shusheng Wang, Jiejing Zhang
The straw can be degraded efficiently into humus by powerful enzymes from microorganisms, resulting in the accelerated circulation of N,P,K and other effective elements in ecological system. We isolated a strain through screening the straw degradation strains from natural humic straw in the low temperature area in northeast of china, which can produce cellulase efficiently. The strain was identified as Pseudomonas mendocina by using morphological, physiological, biochemical test, and molecular biological test, with the functional clarification on producing cellulase for Pseudomonas mendocina for the first time...
September 2, 2016: Bioengineered
Barbara Leśniewska, Marta Krymska, Ewelina Świerad, Józefa Wiater, Beata Godlewska-Żyłkiewicz
A fast ultrasound-assisted sequential extraction (UASE) procedure for the determination of cadmium, lead and nickel fractions in soil was developed and fully validated. The working parameters of an ultrasound probe were optimized by comparing the content of metals in soil extracts obtained by the UASE procedure with that obtained by the conventional (with the aid of a vertical rotor) modified Community Bureau of Reference (BCR) procedure. The content of metals in soil fractions was determined by electrothermal atomic absorption spectrometry...
December 2016: Environmental Science and Pollution Research International
Sang-Yong Eom, Ji-Ae Lim, Yong-Dae Kim, Byung-Sun Choi, Myung Sil Hwang, Jung-Duck Park, Heon Kim, Ho-Jang Kwon
This study was performed to select single nucleotide polymorphisms (SNPs) related to the body burden of heavy metals in Koreans, to provide Korean allele frequencies of selected SNPs, and to assess the difference in allele frequencies with other ethnicities. The candidate-gene approach method and genome-wide association screening were used to select SNPs related to the body burden of heavy metals. Genotyping analysis of the final 192 SNPs selected was performed on 1,483 subjects using the VeraCode Goldengate assay...
July 2016: Toxicological Research
Yan He, Jian Xu, Changsheng Guo, Jiapei Lv, Yuan Zhang, Wei Meng
Contaminants accumulated in sediments may directly harm benthic organisms, however, the specific contaminants responsible for adverse effects have been poorly described. In this study, a bioassay-directed analysis combined with toxicity tests and chemistry analysis was conducted to determine the compounds eliciting the greatest toxicological effect in the sediments in Liaohe River, northeast China. A total of 24 sediment samples were examined to determine their acute toxicity to midge Chironomus tentans (C...
December 2016: Environmental Pollution
Dieter Melchart, Stefan Hager, Jingzhang Dai, Wolfgang Weidenhammer
BACKGROUND: The use of drugs derived from plants is a cornerstone of Traditional Chinese Medicine (TCM). Yet, too little is known about risk and safety of Chinese medicinal drugs (CMD). Therefore, the TCM hospital Bad Kötzting has developed a quality control and complication screening programme in order to ensure a safe administration of TCM drugs to their patients. METHODS: All Chinese medicinal drugs delivered to the hospital between September 1, 2012 and December 31, 2013 entered the quality control program and were screened for microbial contamination, aflatoxin, pesticides and heavy metals...
2016: Forschende Komplementärmedizin, Research in Complementary Medicine
Mohammad Amin Rezvanfar, Mahshid Hodjat, Mohammad Abdollahi
Developmental toxicology is an important area of novel toxicology. In recent years, there have been big concerns toward the increasing exposure to pharmaceutical agents, food additives, pesticides, occupational toxicants, and environmental pollutants, as well as their possible association with all aspects of male or female-mediated transient or permanent defects in progeny. Therefore, it is of great importance to look for new predictive models to evaluate environmental toxicants before they can harm the human health and embryo development...
August 1, 2016: Life Sciences
Bilal Hussain, Tayyaba Sultana, Salma Sultana, Shahid Mahboob, K A Al-Ghanim, Shahid Nadeem
A large number of methods have been applied to evaluate genotoxic damage in different aquatic species. Comet assay, as a method for detecting DNA alterations, and micronucleus test, as an index of chromosomal damage are the most widely used and authentic methods in laboratory and field studies. The primary objective of the study was to evaluate the genotoxic effects of heavy metals generated by toxic industrial effluents and various kinds of pollutants from urban and agricultural areas and domestic waste on Catla catla and Cirrhinus mrigala due to water pollution in the Chenab River, Pakistan...
July 2016: Environmental Science and Pollution Research International
Hidekazu Arai, Katsuo Nakamura, Yasuko Yoshida, Kei Tawarada
Cadmium is a highly toxic heavy metal for humans. In 2006, the Codex Alimentarius Commission set the international standard value of Cd contained in polished rice at 0.4 mg kg(-1), thus requiring the monitoring of Cd in food. We developed two types of Cd immunochromatographic kit by using anti-(Cd-EDTA) antibody. One is the quantitative Cd immunochromatographic device which can precisely measure Cd concentrations. The other is the Cd screening strip that enables visible evaluation of Cd exceeding 0.003 mg L(-1)...
September 2016: Analytical and Bioanalytical Chemistry
Guojian Liao, Qianhua Wu, Renwei Feng, Junkang Guo, Ruigang Wang, Yingming Xu, Yongzhen Ding, Zhilian Fan, Liangyu Mo
Paddy soils in many regions of China have been seriously polluted by multiple heavy metals or metalloids, such as arsenic (As), cadmium (Cd) and lead (Pb). In order to ensure the safety of food and take full advantage of the limited farmland resources of China, exploring an effective technology to repair contaminated soils is urgent and necessary. In this study, three technologies were employed, including variety screening, water management and foliage dressing, to assess their abilities to reduce the accumulation of Cd and As in the grains of different rice varieties, and meanwhile monitor the related yields...
April 1, 2016: Journal of Environmental Management
Salvador Moncho, Jochen Autschbach
A benchmark study for relativistic density functional calculations of NMR spin-spin coupling constants has been performed. The test set contained 47 complexes with heavy metal atoms (W, Pt, Hg, Tl, Pb) with a total of 88 coupling constants involving one or two heavy metal atoms. One-, two-, three-, and four-bond spin-spin couplings have been computed at different levels of theory (nonhybrid vs hybrid DFT, scalar vs two-component relativistic). The computational model was based on geometries fully optimized at the BP/TZP scalar relativistic zeroth-order regular approximation (ZORA) and the conductor-like screening model (COSMO) to include solvent effects...
January 12, 2010: Journal of Chemical Theory and Computation
Bao Wang, Jian-xin Yu, Biao Huang, Wen-you Hu, Qing Chang
Portable X-ray fluorescence (PXRF) spectrometer as a new type of equipment for quick test has a prominent prospect, but there are also shortcomings of detection range and limition, therefore this paper studied the suitability of PXRF spectrometer in monitoring soil environmental qualities of heavy metals included Cr, Ni, Cu, Zn, Pb, Cd, As and Hg, the aim of this paper is to screen elements which can be detected by this kind of instrument and evaluate the accuracy of test results. The research method is to test heavy metals contaminated soil samples by PXRF spectrometer, evaluate the accuracy of test results of PXRF compared with inductively coupled plasma mass(ICP-MS), then establish linear regression relationship between analysis results of PXRF and ICP-MS method...
June 2015: Guang Pu Xue Yu Guang Pu Fen Xi, Guang Pu
Bulent Icgen, Fadime Yilmaz
Due to their metal removal ability, bacterial biosorbents can be effectively used for the treatment of wastewaters containing heavy metals. Searching for bacterial biosorbents for hazardous heavy metals like cadmium is a pivotal for remediation efforts. The gene cadA, that mediates resistance to cadmium over an ATP-dependent efflux mechanism, provides a good target for the selection of potential cadmium biosorbents. For this reason, in this study, a 36-mer-oligonucleotide DNA probe based on the entire 3.5-kb BglII-XbaI fragment of cadA operon from staphylococcal plasmid pI258 was prepared by using Vector NTI Express software...
March 2016: Environmental Science and Pollution Research International
Asoka Pathiratne, Chamini K Hemachandra, Nimal De Silva
Efficacy of Allium cepa test system for screening cytotoxicity and genotoxicity of treated effluents originated from four types of industrial activities (two textile industries, three rubber based industries, two common treatment plants of industrial zones, and two water treatment plants) was assessed. Physico-chemical parameters including the heavy metal/metalloid levels of the effluents varied depending on the industry profile, but most of the measured parameters in the effluents were within the specified tolerance limits of Sri Lankan environmental regulations for discharge of industrial effluents into inland surface waters...
December 2015: Environmental Monitoring and Assessment
Elizabeth Silva, María Elsa Villarreal, Omayda Cárdenas, Carlos Armando Cristancho, Carmenza Murillo, Manuel Alberto Salgado, Gerardo Nava
INTRODUCTION: Significant alterations may be found in the water of Bogotá´s water supply system after its purification, specifically during its distribution and storage in home reservoirs, which makes it necessary to study the final quality of the domiciliary water consumed by users. OBJECTIVE: To conduct a preliminary study of toxic chemical substances in the water supplied by Bogotá´s water supply system in samples obtained from residential reservoirs and faucets...
August 2015: Biomédica: Revista del Instituto Nacional de Salud
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