Xiaomin Yang, Zhigang Bai, Yunxin He, Ning Wang, Liangqing Sun, Yongqi Li, Zujun Yin, Xiaoge Wang, Binglei Zhang, Mingge Han, Xuke Lu, Xiugui Chen, Delong Wang, Junjuan Wang, Shuai Wang, Lixue Guo, Chao Chen, Keyun Feng, Wuwei Ye
BACKGROUND: DNA methylation is an important epigenetic mode of genomic DNA modification and plays a vital role in maintaining epigenetic content and regulating gene expression. Cytosine-5 DNA methyltransferase (C5-MTase) are the key enzymes in the process of DNA methylation. However, there is no systematic analysis of the C5-MTase in cotton so far, and the function of DNMT2 genes has not been studied. METHODS: In this study, the whole genome of cotton C5-MTase coding genes was identified and analyzed using a bioinformatics method based on information from the cotton genome, and the function of GhDMT6 was further validated by VIGS experiments and subcellular localization analysis...
April 23, 2024: BMC Plant Biology