Read by QxMD icon Read


Komal Kumar Javarappa, Attemode Girijanna Devi Prasad, A J Mahadesh Prasad, Chetana Mane
BACKGROUND: Kingiodendron pinnatum Rox. Hams. is an endangered medicinal plant used in gonorrhoe, catarrhal conditions of genito-urinary and respiratory tracts. The scientific and pharmacological formulation of K. pinnatum has not been established so far though it is being traditionally used by tribes of the region. OBJECTIVE: P hytochemical screening and identification of the bioactive compounds from the ethyl acetate extract of Kingiodendron pinnatum Rox. Hams...
October 2016: Pharmacognosy Research
I Ayelen Ramallo, Paula García, Ricardo L E Furlan
A dual readout autographic assay to detect acetylcholinesterase inhibitors present in complex matrices adsorbed on reversed-phase or normal-phase thin-layer chromatography plates is described. Enzyme gel entrapment with an amphiphilic copolymer was used for assay development. The effects of substrate and enzyme concentrations, pH, incubation time, and incubation temperature on the sensitivity and the detection limit of the assay were evaluated. Experimental design and response surface methodology were used to optimize conditions with a minimum number of experiments...
November 2015: Journal of Separation Science
I Ayelen Ramallo, Mario O Salazar, Ricardo L E Furlan
INTRODUCTION: The prevailing treatment for Alzheimer's disease is the use of acetylcholinesterase (AChE) inhibitors. Natural extracts are the principal source of AChE's inhibitors. However, their chemical complexity demands for simple, selective and rapid assays. OBJECTIVE: To develop a strategy for identification of AChE inhibitors present in mixtures employing high resolution mass spectrometry (HRMS) and thin layer chromatography (TLC)-biological staining. METHODOLOGY: The strategy uses an autographic assay based on the α-naphthyl acetate - fast blue B system for the detection of AChE activity...
November 2015: Phytochemical Analysis: PCA
Shotaro Okayama, Yasunori Suzuki, Hidemasa Shimpo, Chikahiro Ohkubo
The fixation strengths between conventional/modified magnetic assemblies and denture base resins were evaluated using six alternative resins. Magnetic assemblies with three different undercut wings were prepared. Soft lining materials with added PMMA resin polymer, two photopolymerization denture relining resins, an experimental resin, and a temporary filling resin were used to fix the magnetic assemblies to the denture bases. As a control, a commercially available magnetic assembly without undercut wings and a conventional autopolymerized resin were also prepared...
2015: Dental Materials Journal
Paula García, Ricardo L E Furlan
INTRODUCTION: Autographic methods are useful tools to detect bioactive compounds in complex matrixes. Experimental design and optimisation techniques were implemented for the development of an autographic assay suitable for the detection of tyrosinase inhibitors. OBJECTIVES: To develop an autographic assay to detect tyrosinase inhibitors using gel entrapped enzyme, experimental design and response surface methodology (RSM) to optimise conditions with a minimum number of experiments...
July 2015: Phytochemical Analysis: PCA
D N Orlov, A R Nezvetskiĭ, T G Orlova, O V Petrukhin, N Ia Orlov
The supposition that nucleoside diphosphate kinase is the enzyme that phosphorylates transducin beta-subunits on one of the histidine residues (His-266) has been analyzed. It stands the reason that 1) this enzyme is multifunctional and plays in particular the role of protein histidine kinase; and 2) the phosphorylated beta-subunit of transducin may activate transducin via the mechanism of transphosphorylation. Nevertheless, in our experiments, in which different forms of transducin preparations were incubated with α- and β-isoforms of recombinant rat NDP kinase in the presence of [γ32P]ATP or [γ32P]GTP (specific activity of about 1 Ci/mmol) followed by separation of proteins by electrophoresis and-gel radio-autography, the phosphorylation of the transducin beta-subunit wasn't succeeded to be found...
September 2014: Biofizika
Nancy Patience Motlalepula Komape, Mutalib Aderogba, Victor Patrick Bagla, Peter Masoko, Jacobus Nicolaas Eloff
BACKGROUND: Combretum vendae A.E. van Wyk (Combretaceae) is used for the treatment of bacterial related infections and oxidative related diseases by indigenous people of South Africa. Dried leaves extracts of C. vendae were investigated for bioactivity against a variety of bacterial strains and their antioxidant potential evaluated. MATERIALS AND METHODS: Constituents of leaf material were serially extracted using solvents of varying polarities, TLC chromatograms of the fractions were sprayed with 2,2 diphenyl-1-picrylhydrazyl (DPPH) to determine the presence of antioxidant compounds...
2014: African Journal of Traditional, Complementary, and Alternative Medicines: AJTCAM
Manoj K Dalai, Santanu Bhadra, Sushil K Chaudhary, Arun Bandyopadhyay, Pulok K Mukherjee
BACKGROUND: Clove (Syzygium aromaticum) is a well-known culinary spice with strong aroma; contains a high amount of oil known as clove oil. The major phyto-constituent of the clove oil is eugenol. Clove and its oil possess various medicinal uses in indigenous medicine as an antiseptic, anti-oxidant, analgesic and neuroprotective properties. Thus, it draws much attention among researchers from pharmaceutical, food and cosmetic industries. OBJECTIVE: The aim of the present study was to determine the anti-cholinesterase activity of the methanol extract of clove, its oil and eugenol...
April 2014: Pharmacognosy Magazine
Tom Wileman
Autophagy is a membrane trafficking pathway that results in the formation of autophagosomes which deliver portions of the cytosol to lysosomes for degradation. When autophagosomes engulf intracellular pathogens, the pathway is called 'xenophagy' because it leads to the removal of foreign material. Autophagy is activated during infection by Toll-like receptors that recognize pathogen-associated molecular patterns. This allows autophagy to kill micro-organisms and present pathogen components to the innate and acquired immune systems...
2013: Essays in Biochemistry
Ji-dong Jia
No abstract text is available yet for this article.
February 2013: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
Gaurav Rajauria, Nissreen Abu-Ghannam
Seaweeds are important sources of carotenoids, and numerous studies have shown the beneficial effects of these pigments on human health. In the present study, Himanthalia elongata brown seaweed was extracted with a mixture of low polarity solvents, and the crude extract was separated using analytical thin-layer chromatography (TLC). The separated compounds were tested for their potential antioxidant capacity and antimicrobial activity against Listeria monocytogenes bacteria using TLC bioautography approach...
2013: International Journal of Analytical Chemistry
Léocadie Kamagaju, Renato Morandini, Elias Bizuru, Polycarpe Nyetera, Jean Baptiste Nduwayezu, Caroline Stévigny, Ghanem Ghanem, Pierre Duez
ETHNOPHARMACOLOGICAL RELEVANCE: Traditional herbal medicines provide an interesting, largely unexplored source for the development of potential new drugs and skin-care cosmetics. Some herbal extracts are known to be inhibitors of melanin formation, sometimes more potent than the classical inhibitors, hydroquinone/arbutin or kojic acid, and are not associated with melanocytes cytotoxicity or mutagenicity. Such plants are used in traditional medicine in many countries, particularly in Africa, for skin lightening...
April 19, 2013: Journal of Ethnopharmacology
Kai Diederich, Verena Quennet, Henrike Bauer, Harald D Müller, Heike Wersching, Wolf-Rüdiger Schäbitz, Jens Minnerup, Clemens Sommer
BACKGROUND AND PURPOSE: Both application of granulocyte-colony stimulating factor (G-CSF) and constraint-induced movement therapy (CIMT) have been shown to improve outcome after experimental stroke. The aim of the present study was to determine whether concurrent or sequential combination of both therapies will further enhance therapeutic benefit and whether specific modifications in the abundance of various neurotransmitter receptors do occur. METHODS: Adult male Wistar rats were subjected to photothrombotic ischemia and assigned to the following treatment groups (n=20 each): (1) ischemic control (saline); (2) CIMT (CIMT between poststroke Days 2 and 11; (3) G-CSF (10 μg/kg G-CSF daily between poststroke Days 2 and 11; (4) combined concurrent group (CIMT plus 10 μg/kg G-CSF daily between poststroke Days 2 and 11; and (5) combined sequential group (CIMT between poststroke Days 2 and 11 and 10 μg/kg G-CSF daily between poststroke Days 12 and 21, respectively)...
January 2012: Stroke; a Journal of Cerebral Circulation
Ian Williams
Over the last three decades, the graphic novel has developed both in sophistication and cultural importance, now being widely accepted as a unique form of literature (Versaci 2007). Autobiography has proved to be a successful genre within comics (the word is used in the plural to denote both the medium and the philosophy of the graphic form) and within this area a sub-genre, the memoir of the artist's own disease or suffering, sometimes known as the graphic pathology, has arisen (Green and Myers 2010). Storytelling and healing have been linked since ancient times, and the disclosure of ones story forms part of the psychotherapeutic treatment of trauma (Herman 1997)...
December 2011: Journal of Medical Humanities
M Colucci, M Coluccia, P Montemurro, M Conese, A Nassi, F Loseto, E Alessio, G Mestroni, N Semeraro
Some Ru(II)-DMSO complexes have antimetastatic properties in experimental tumors. Since plasminogen activators are thought to play an important role in the expression of cancer cell metastatic capacity, we evaluated the effect of two Ru(II)-DMSO complexes on the fibrinolytic activity of Lewis lung carcinoma. Tumor-bearing mice were given daily, for 14 days, an i.p. injection of antimetastatic dosages of cis-RuCl2(DMSO)4 (700 mg/kg/die) or trans-RUCl2(DMSO)4 (37 mg/kg/die), or vehicle. Tumor extracts obtained on day 15 from treatment groups had significantly lower (plasminogen-dependent) fibrinolytic activity than extracts from control animals (p<0...
April 1993: International Journal of Oncology
No abstract text is available yet for this article.
May 1946: Archives of Pathology
No abstract text is available yet for this article.
November 1946: Proceedings of the Society for Experimental Biology and Medicine
No abstract text is available yet for this article.
May 1947: Anatomical Record
Yan Sun, Chao Wang, Jiapei Dai
Cell to cell communication by biophotons has been demonstrated in plants, bacteria, animal neutrophil granulocytes and kidney cells. Whether such signal communication exists in neural cells is unclear. By developing a new biophoton detection method, called in situ biophoton autography (IBA), we have investigated biophotonic activities in rat spinal nerve roots in vitro. We found that different spectral light stimulation (infrared, red, yellow, blue, green and white) at one end of the spinal sensory or motor nerve roots resulted in a significant increase in the biophotonic activity at the other end...
March 2010: Photochemical & Photobiological Sciences
Abdelaaty A Shahat, Gamal El-Barouty, Rasmeia A Hassan, Fiza M Hammouda, Fawzia H Abdel-Rahman, Mahmoud A Saleh
Seeds of Enterolobium contortisiliquum were subjected to steam distillation to obtain a light yellow essential oil in a yield of 3 ml/kg of seeds. The major components of the oil were identified using gas chromatography/mass spectrometry (GC-MS) and were furfural, limonene, linalool, estragole, carvone, and apiole with carvone representing more than 50% of the total composition. Antimicrobial activities of the essential oil were determined against four species of gram positive bacteria (Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Micrococcus luteus) and two gram negative bacteria (Klebsiella pneumoniae, Serratia Marcescencs)...
August 2008: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"