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https://www.readbyqxmd.com/read/29666220/synergistic-anti-proliferative-effect-of-mtor-inhibitor-rad001-plus-gemcitabine-on-cholangiocarcinoma-by-decreasing-choline-kinase-activity
#1
Gigin Lin, Kun-Ju Lin, Frank Wang, Tse-Ching Chen, Tzu-Chen Yen, Ta-Sen Yeh
Although gemcitabine plus cisplatin is the gold standard chemotherapy regimen for advanced cholangiocarcinoma, the response rate has been disappointing. This study aims to investigate a novel therapeutic regimen (gemcitabine plus rad001, an mTOR inhibitor) for cholangiocarcinoma. Gemcitabine, oxaliplatin, cetuximab, and rad001 in various combinations were first evaluated in vitro using six cholangiocarcinoma cell lines. In vivo therapeutic efficacies of gemcitabine, rad001 alone and combination were further evaluated using a xenograft mouse model and a chemically induced orthotopic cholangiocarcinoma rat model...
April 12, 2018: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/29661104/effect-of-micro-roughening-of-poly-ether-ether-ketone-on-bone-marrow-derived-stem-cell-and-macrophage-responses-and-osseointegration
#2
Sunarso, Akira Tsuchiya, Naoyuki Fukuda, Riki Toita, Kanji Tsuru, Kunio Ishikawa
Poly(ether ether ketone) (PEEK) has emerged as a candidate to replace metal implants because of its satisfactory mechanical properties, radiolucency, and lack of metal allergy. However, PEEK lacks osseointegration ability limiting its clinical applications. To overcome this problem, we prepared PEEK with a micro-rough surface using the sandblast method to modulate its osseointegration property; the sandblast method is simple, cost-effective, and is already applied to clinical metal implants. The surface roughness of the sandblasted PEEK was about 2...
April 16, 2018: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/29660943/circulating-cell-free-micro-rna-profiles-reflect-discordant-development-of-dementia-in-monozygotic-twins
#3
Jonas Mengel-From, Mette E Rønne, Anting L Carlsen, Kristin Skogstrand, Lisbeth A Larsen, Qihua Tan, Lene Christiansen, Kaare Christensen, Niels H H Heegaard
We aim to examine if circulating micro-RNA and cytokine levels associate with dementia diagnosis and cognitive scores. To test our hypothesis, we use plasma donated from 48 monozygotic twin pairs in 1997 and 46 micro-RNAs and 10 cytokines were quantified using microfluidic RT-qPCR and multiplex solid-phase immunoassays, respectively. Micro-RNA and cytokine profiling were examined for associations with dementia diagnoses in a longitudinal registry study or with cognitive scores at baseline. Thirty-six micro-RNAs and all cytokines were detected consistently...
April 11, 2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/29660339/glut5-increases-fructose-utilization-and-promotes-tumor-progression-in-glioma
#4
Chunhai Su, Hui Li, Wenbo Gao
Fructose is now such an important component of human diets, and several studies have found that some cancer cells could utilize fructose to overcome low glucose micro-environment, but the study on the role of fructose in glioma is rare. To explore the role of fructose in glioma, we detected the proliferation and colony formation ability of glioma cells in fructose medium, and found that the abilities of proliferation and colony formation of glioma cells in fructose medium were similar with abilities in glucose medium, however, fructose just partly restored proliferation ability of normal glial cells...
April 13, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29659812/the-genome-sequence-and-transcriptome-of-potentilla-micrantha-and-their-comparison-to-fragaria-vesca-the-woodland-strawberry
#5
Matteo Buti, Marco Moretto, Elena Barghini, Flavia Mascagni, Lucia Natali, Matteo Brilli, Alexandre Lomsadze, Paolo Sonego, Lara Giongo, Michael Alonge, Riccardo Velasco, Claudio Varotto, Nada Šurbanovski, Mark Borodovsky, Judson A Ward, Kristof Engelen, Andrea Cavallini, Alessandro Cestaro, Daniel James Sargent
Background: The genus Potentilla is closely related to that of Fragaria, the economically important strawberry genus. Potentilla micrantha is a species that does not develop berries but shares numerous morphological and ecological characteristics with Fragaria vesca. These similarities make P. micrantha an attractive choice for comparative genomics studies with F. vesca. Findings: In this study, the P. micrantha genome was sequenced and annotated, and RNA-Seq data from the different developmental stages of flowering and fruiting were used to develop a set of gene predictions...
April 1, 2018: GigaScience
https://www.readbyqxmd.com/read/29659744/human-adipose-mesenchymal-stem-cells-overexpressing-dual-chemotactic-gene-showed-enhanced-angiogenic-capacity-in-ischemic-hindlimb-model
#6
Younggeul Min, Seongho Han, Hyun Aae Ryu, Sung-Whan Kim
Aims: In present study, we sought to characterize the angio-vasculogenic property of human adipose mesenchymal stem cells (ASCs) overexpressing dual chemokine GCP-2 and SDF-1α (ASC-G/S) and to determine the therapeutic potential of ASC-G/S in the context of experimental ischemia. Methods and results: We generated ASC-G/S line and performed flow cytometry, quantitative (q)-PCR, Matrigel tube formation, Matrigel plug assays and in vivo therapeutic assays using hind limb ischemia mouse model...
April 5, 2018: Cardiovascular Research
https://www.readbyqxmd.com/read/29659697/variations-in-leaf-growth-parameters-within-the-tree-structure-of-adult-coffea-arabica-in-relation-to-seasonal-growth-water-availability-and-air-carbon-dioxide-concentration
#7
Miroslava Rakocevic, Fabio Takeshi Matsunaga
Background and Aims: Dynamics in branch and leaf growth parameters, such as the phyllochron, duration of leaf expansion, leaf life span and bud mortality, determine tree architecture and canopy foliage distribution. We aimed to estimate leaf growth parameters in adult Arabica coffee plants based on leaf supporter axis order and position along the vertical profile, considering their modifications related to seasonal growth, air [CO2] and water availability. Methods: Growth and mortality of leaves and terminal buds of adult Arabica coffee trees were followed in two independent field experiments in two sub-tropical climate regions of Brazil, Londrina-PR (Cfa) and Jaguariúna-SP (Cwa)...
April 5, 2018: Annals of Botany
https://www.readbyqxmd.com/read/29659195/mir-541-suppresses-proliferation-and-invasion-of-squamous-cell-lung-carcinoma-cell-lines-via-directly-targeting-high-mobility-group-at-hook-2
#8
Li Xu, Bin Du, Qi-Jue Lu, Xiao-Wen Fan, Ke Tang, Lie Yang, Wei-Lin Liao
An increasing number of studies have demonstrated that micro-ribonucleic acids (miRNAs) are important tumor suppressors during carcinogenesis. However, the function of miRNA-541 (miR-541) in malignancies, especially lung cancer, has not been widely reported. In this study, miR-541 expression was significantly decreased in squamous cell lung carcinoma (SCLC) cancerous tissue and SCLC cell lines. To analyze miR-541 function in SCLC, we overexpressed miR-541 in SCLC cell lines (SK-MES-1 and H226). According to the CCK8, wound scratch, and transwell invasion assay results, miR-541 overexpression significantly inhibited SCLC cell proliferation, migration, and invasion ability...
April 16, 2018: Cancer Medicine
https://www.readbyqxmd.com/read/29658582/sirt1-nrf2-signalling-pathway-prevents-cognitive-impairment-in-diabetic-rats-through-anti%C3%A2-oxidative-stress-induced-by-mirna%C3%A2-23b%C3%A2-3p-expression
#9
Jinhua Han, Xiaoning Liu, Yingzhi Li, Jun Zhang, Haichi Yu
In the present study the exact roles and mechanisms underlying the effect of miRNA‑23b‑3p on the cognitive impairment of diabetic rats were investigated. The in vivo model of diabetes was established in Wistar rats via a single injection of streptozotocin (STZ). Cognitive function was evaluated using a Morris water maze test. Oxidative stress was measured using ELISA kits, and the protein expression levels of B‑cell lymphoma 2‑associated X protein, silent information regulator 1 (SIRT1), nuclear factor erythroid 2‑related factor 2 (Nrf2) and GAPDH were measured by western blot analysis...
April 12, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29652608/immunomodulatory-role-of-stem-cell-from-human-exfoliated-deciduous-teeth-on-periodontal-regeneration
#10
Xianling Gao, Zongshan Shen, Meiliang Guan, Qiting Huang, Lingling Chen, Wei Qin, Xiaohu Ge, Haijia Chen, Yin Xiao, Zhengmei Lin
Periodontitis is initiated by the infection of periodontal bacteria and subsequent tissue inflammation due to immunoreaction, eventually leading to periodontal apparatus loss. Stem cells from human exfoliated deciduous teeth (SHEDs) have exhibited beneficial characteristics in dental tissue regeneration. However, the immunomodulatory functions of SHEDs have not been elucidated in the context of periodontitis treatment. In this study, we investigated the potential immunomodulatory effects of SHEDs on experimental periodontitis and demonstrated that multi-dose delivery of SHEDs led to periodontal tissue regeneration...
March 20, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29651937/an-emerging-role-of-endometrial-inflammasome-in-reproduction-new-therapeutic-approaches
#11
Fiorella Di Nicuolo, Monia Specchia, Lorenza Trentavizi, Giovanni Scambia, Nicoletta Di Simone
One of the common complications of pregnancy is spontaneous pregnancy loss which occurs in an estimated 5- 15% of pregnancies. Of all women 1%-5% suffer from recurrent pregnancy loss (RPL). Despite the fact that RPL has been associated to various anatomic, hormonal, immune, hematologic, and genetic defects, in 30% of the patients, screening tests included in the RPL workup may have negative results. Recently, we demonstrated a significant increased activation of endometrial NALP-3 inflammasome, and a caspase-1 dependent secretion of IL-18 and IL-1β in the endometrial tissues obtained from RPL women compared with a fertile women group...
April 12, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29651108/3d-artificial-round-section-micro-vessels-to-investigate-endothelial-cells-under-physiological-flow-conditions
#12
Riccardo Sfriso, Shengye Zhang, Colette Andrea Bichsel, Oliver Steck, Alain Despont, Olivier Thierry Guenat, Robert Rieben
In the context of xenotransplantation, in ischemia/reperfusion injury as well as in cardiovascular research, the study of the fascinating interplay between endothelial cells (EC) and the plasma cascade systems often requires in vitro models. Blood vessels are hardly reproducible with standard flat-bed culture systems and flow-plate assays are limited in their low surface-to-volume ratio which impedes the study of the anticoagulant properties of the endothelial cells. According to the 3R regulations (reduce, replace and refine animal experimentation) we developed a closed circuit microfluidic in vitro system in which endothelial cells are cultured in 3D round section microchannels and subjected to physiological, pulsatile flow...
April 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29649432/nitric-oxide-signaling-regulates-tumor-induced-intussusceptive-like-angiogenesis
#13
Selvaraj Vimalraj, Srinivasan Bhuvaneswari, Sundaresan Lakshmikirupa, Ganesh Jyothsna, Suvro Chatterjee
Existing animal models for screening tumor angiogenic process have various setbacks that necessitate further investigations. In this study, we developed an ex-ovo egg yolk angiogenesis model to screen the angiogenic potency of tumor cells (HeLa and SiHa cell lines). The egg yolk angiogenesis assay was applied to study the nitric oxide (NO) influence on switching from sprouting angiogenesis (SA) to intussusceptive angiogenesis (IA) under tumor microenvironment. Morphological analysis and SA-like or IA-like markers expression were determined during the development of chicken chorioallantoic membrane (CAM) from day 5 to 13...
April 9, 2018: Microvascular Research
https://www.readbyqxmd.com/read/29649408/interferon-gamma-impairs-maintenance-and-alters-hematopoietic-support-of-bone-marrow-mesenchymal-stromal-cells
#14
Marieke Goedhart, Anne Cornelissen, Carlijn Kuijk, Sulima Geerman, Marion Kleijer, Jaap D van Buul, Stephan Huveneers, Marc Raaijmakers, Howard A Young, Monika C Wolkers, Carlijn Voermans, Martijn A Nolte
Bone marrow (BM) mesenchymal stromal cells (MSCs) provide micro-environmental support to hematopoietic stem and progenitor cells (HSPCs). Culture-expanded MSCs are interesting candidates for cellular therapies due to their immunosuppressive and regenerative potential which can be further enhanced by pre-treatment with interferon-gamma (IFN-γ). However, it remains unknown whether IFN-γ can also influence hematopoietic support by BM-MSCs. Here, we elucidate the impact of IFN-γ on the hematopoietic support of BM-MSCs...
March 12, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29648890/phelligridin-d-loaded-oral-nanotube-titanium-implant-enhances-osseointegration-and-prevents-osteolysis-in-rat-mandible
#15
Ji-Eun Kim, Jyoti Shrestha Takanche, Jeong-Seok Kim, Min-Ho Lee, Jae-Gyu Jeon, Il-Song Park, Ho-Keun Yi
Poor bone quality and osteolysis are the major causes of implant failure in dentistry. Here, this study tested the effect of phelligridin D-loaded nanotubes titanium (Ti) for bone formation around the dental implants. The purpose of this study was to enhance osseointegration of phelligridin D-loaded implant into the bone for bone formation and prevention of osteolysis. Cell viability, crystal violet staining, Western blot, alizarin red S staining, alkaline phosphatase activity, tartrate-resistant acid phosphatase staining, micro-computed tromography (μ-CT), hematoxylin and eosin (H&E) and immunohistochemical staining were used in vitro and in vivo to test the biocompatibility of phelligridin D...
April 12, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29627937/poor-prognosis-in-epstein-barr-virus-negative-gastric-cancer-with-lymphoid-stroma-is-associated-with-immune-phenotype
#16
Charles J Cho, Hyo Jeong Kang, Yeon-Mi Ryu, Young Soo Park, Hui Jeong Jeong, Young-Mi Park, Hyun Lim, Jeong Hoon Lee, Ho June Song, Hwoon-Yong Jung, Sang-Yeob Kim, Seung-Jae Myung
BACKGROUND: Gastric cancer with lymphoid stroma (GCLS) is pathologically characterized by poorly developed tubular structures with a prominent lymphocytic infiltration. Its clinical and prognostic features differ in patients positive and negative for Epstein-Barr virus (EBV) infection. This study analyzed the expression of programmed cell death-1 (PD-1), programmed cell death ligand-1 (PD-L1), and the density of tumor-infiltrating lymphocytes (TILs) including CD3+ and CD8+ T cells, as well as their prognostic significance in patients with GCLS...
April 7, 2018: Gastric Cancer
https://www.readbyqxmd.com/read/29622042/engineering-crispr-interference-system-in-klebsiella-pneumoniae-for-attenuating-lactic-acid-synthesis
#17
Jingxuan Wang, Peng Zhao, Ying Li, Lida Xu, Pingfang Tian
BACKGROUND: Klebsiella pneumoniae is a promising industrial species for bioproduction of bulk chemicals such as 1,3-propanediol, 2,3-butanediol and 3-hydroxypropionic acid (3-HP). However, lactic acid is a troublesome by-product when optimizing for 3-HP production. Therefore, it is highly desirable to minimize lactic acid. RESULTS: Here, we show that lactic acid synthesis can be largely blocked by an engineered CRISPR interference (CRISPRi) system in K. pneumoniae...
April 5, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29621968/effects-of-micro-rnas-and-their-targets-in-periodontal-diseases
#18
Samane Bourbour, Maryam Beheshti, Hossein Kazemian, Abbas Bahador
: RNA interference is a strong mechanism of gene silencing which underlies many fields of eukaryotic biology (1). MicroRNAs (miRNAs) are small noncoding RNAs and a gene regulation expression system showing a tissue-specific expression pattern, and inhibits protein synthesis (2). For the first time, miRNA has been seen in Caenorhabditis elegans (3). They play an important role in signaling pathways of various diseases especially periodontal disease and cancers (4). One of the most important diseases in association with gingival infectious is periodontal diseases, which if untreated leads to tooth loss, hence in present paper focus on the effects of MicroRNAs and their targets in disease which was mentioned above...
April 5, 2018: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/29621288/low-power-laser-irradiation-and-human-adipose-derived-stem-cell-treatments-promote-bone-regeneration-in-critical-sized-calvarial-defects-in-rats
#19
Yan-Hsiung Wang, Jyun-Yi Wu, Su Chii Kong, Min-Hsuan Chiang, Mei-Ling Ho, Ming-Long Yeh, Chia-Hsin Chen
Both stem cell therapy and physical treatments have been shown to be beneficial in accelerating bone healing. However, the efficacy of combined treatment with stem cells and physical stimuli for large bone defects remains uncertain. The aim of this study was to evaluate the bone regeneration effects of low-power laser irradiation (LPLI) and human adipose-derived stem cell (ADSC) treatments during fracture repair using a comparative rat calvarial defect model. We evaluated the viability of human ADSCs, which were cultured on a porous PLGA scaffold using an MTS assay...
2018: PloS One
https://www.readbyqxmd.com/read/29620241/microarray-expression-profile-analysis-of-circular-rnas-in-pancreatic-cancer
#20
Shixiang Guo, Xuejun Xu, Yongsheng Ouyang, Yunchao Wang, Jiali Yang, Liangyu Yin, Jiayun Ge, Huaizhi Wang
Pancreatic cancer remains one of the most malignant tumors with a poor prognosis. Despite advances in diagnosis and treatment, no reliable biomarkers are available for clinical practice. Circular RNAs (circRNAs) are a novel class of endogenous non‑coding RNA, which are abundant, stable and conserved, and serve crucial roles in disease, particularly in cancer. The purpose of the present study was to investigate the expression profile of circRNAs in 20 pancreatic cancer tissues and corresponding paracancerous tissues using arraystar human circRNA array analysis, high‑throughput circRNA microarray, bioinformatic analysis and reverse transcription‑quantitative polymerase chain reaction...
March 29, 2018: Molecular Medicine Reports
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