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https://www.readbyqxmd.com/read/29028357/folate-peg-nota-al18f-a-new-folate-based-radiotracer-for-pet-imaging-of-folate-receptor-positive-tumors
#1
Qingshou Chen, Xiangjun Meng, Paul McQuade, Daniel Rubins, Shu-An Lin, Zhizhen Zeng, Hyking Haley, Patricia Miller, Dinko Gonzalez Trotter, Philip S Low
The folate receptor (FR) has been established as a promising target for imaging and therapy of cancer (FR-α), inflammation, and autoimmune diseases (FR-β). Several folate-based PET radiotracers have been reported in the literature, but an 18F- labeled folate-PET imaging agent with optimal properties for clinical translation is still lacking. In the present study, we report the design and preclinical evaluation of folate-PEG12-NOTA-Al18F (1), a new folate-PET agent with improved potential for clinical applications...
October 13, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29025984/synthesis-and-biological-evaluation-of-a-novel-18-f-labeled-adnectin-as-a-pet-radioligand-for-imaging-pd-l1-expression
#2
David J Donnelly, Ralph Adam Smith, Paul Morin, Dasa Lipovsek, Jochem Gokemeijer, Daniel Cohen, Virginie Lafont, Tritin Tran, Erin Lee Cole, Martin Wright, Joonyoung Kim, Adrienne Pena, Daniel Kukral, Douglas D Dischino, Patrick Chow, Jinping Gan, Olufemi Adelakun, Xi-Tao Wang, Kai Cao, David Lueng, Samuel Bonacorsi, Wendy Hayes
The programmed death protein (PD-1) and its ligand (PD-L1) play critical roles in a checkpoint pathway cancer cells exploit to evade the immune system. A same-day PET imaging agent for measuring PD-L1 status in primary and metastatic lesions could be important for optimizing drug therapy. Herein, we have evaluated the tumor targeting of an anti-PD-L1 Adnectin after (18)F-fluorine labeling. Methods: An anti-PD-L1 Adnectin was labeled with (18)F in two steps. This synthesis featured fluorination of a novel prosthetic group, followed by a copper-free click conjugation to a modified Adnectin to generate (18)F-BMS-986192...
October 12, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/29024853/overexpression-of-constitutively-active-map3k7-in-ameloblasts-causes-enamel-defects-of-mouse-teeth
#3
Zhao Jinping, Chu Qing, Song Wenying, Yang Chunyan, Xiang Lili, Shi Yao, Wang Yumin, Xu Zhenzhen, Zhang Li, Gao Yuguang
OBJECTIVE: Compelling evidence suggests that mitogen-activated protein kinases (Mapks) play an important role in amelogenesis. However, the role of transforming growth factor (TGF)-β-activating kinase 1 (Tak1, Map3k7), which is a known upstream kinase of Mapks, during amelogenesis remains to be determined. The aim of this study was to investigate the possible involvement of Map3k7 in amelogenesis. DESIGN: We generated transgenic mice that produced constitutively active human MAP3K7 (caMAP3K7) under the control of amelogenin (Amelx) gene promoter...
September 25, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29019955/mechanical-and-electrical-noise-in-sense-channel-of-mems-vibratory-gyroscopes
#4
Xukai Ding, Jia Jia, Yang Gao, Hongsheng Li
This paper presents a theoretical analysis of mechanical and electrical noise in the sense channel of micro-electromechanical systems (MEMS) vibratory gyroscopes. Closed-form expressions for the power spectral density (PSD) of the noise equivalent rate (NER) of gyroscopes in the open-loop and the force-rebalance operations are derived by using an averaged PSD model and an equivalent transfer function. The obtained expressions are verified through numerical simulations, demonstrating close agreements between the analytic and the numerical models...
October 11, 2017: Sensors
https://www.readbyqxmd.com/read/29017848/suppression-of-cotton-leaf-curl-disease-symptoms-in-gossypium-hirsutum-through-overexpression-of-host-encoded-mirnas
#5
Mohd Akmal, Mirza S Baig, Jawaid A Khan
Cotton leaf curl disease (CLCuD), a major factor resulting in the enormous yield losses in cotton crop, is caused by a distinct monopartite begomovirus in association with Cotton leaf curl Multan betasatellite (CLCuMB). Micro(mi)RNAs are known to regulate gene expression in eukaryotes, including antiviral defense in plants. In a previous study, we had computationally identified a set of cotton miRNAs, which were shown to have potential targets in the genomes of Cotton leaf curl Multan virus (CLCuMuV) and CLCuMB at multiple loci...
October 7, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28994235/lipo-protein-emulsion-structure-in-the-diet-affects-protein-digestion-kinetics-intestinal-mucosa-parameters-and-microbiota-composition
#6
Marion Oberli, Véronique Douard, Martin Beaumont, Daphné Jaoui, Fabienne Devime, Sandy Laurent, Catherine Chaumontet, Damien Mat, Steven Le Feunteun, Camille Michon, Anne-Marie Davila, Gilles Fromentin, Daniel Tomé, Isabelle Souchon, Marion Leclerc, Claire Gaudichon, François Blachier
SCOPE: Food structure is a key factor controlling digestion and nutrient absorption. We tested the hypothesis that protein emulsion structure in the diet may affect digestive and absorptive processes. METHODS & RESULTS: Rats (n = 40) were fed for 3 weeks two diets chemically identical but based on lipid-protein liquid-fine (LFE) or gelled-coarse (GCE) emulsions that differ at the macro- and micro-structure levels. After an overnight fasting, they ingested a (15) N-labeled LFE or GCE test meal and were euthanized 0, 15min, 1h and 5h later...
October 10, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28993426/effect-of-diabetes-status-and-hyperglycemia-on-global-dna-methylation-and-hydroxymethylation
#7
Jairo Arturo Pinzón-Cortés, Angelina Perna-Chaux, Nicolás Steven Rojas-Villamizar, Angelica Diaz-Basabe, Diana Carolina Polania-Villanueva, María Fernanda Jacome, Carlos Olimpo Mendivil, Helena Groot, Valeriano López-Segura
Type 2 diabetes mellitus (T2DM) is characterized by oxidative stress that could lead to chronic micro- and macro-vascular complications. We hypothesized that some of the target organ damage is mediated by oxidative alterations in epigenetic mechanisms involving DNA methylation (5mC) and DNA hydroxymethylation (5hmC). We analyzed global DNA methylation and hydroxymethylation in peripheral blood cells in well-controlled and poorly controlled patients with type 2 diabetes mellitus and compared them to healthy controls...
October 9, 2017: Endocrine Connections
https://www.readbyqxmd.com/read/28990218/a-polysaccharide-from-dictyophora-indusiata-inhibits-the-immunosuppressive-function-of-cancer-associated-fibroblasts
#8
Shuyu Han, Chaoxiong Ma, Minghua Hu, Yuanyuan Wang, Fangli Ma, Ning Tao, Zhihai Qin
Reversing the function of cancer-associated fibroblasts (CAFs) may improve the efficacy of cancer therapy. Here, we isolated a novel polysaccharide from Dictyophora indusiata (ZSP4) and examined its effects on the function of prostate CAFs. The supernatant of prostate CAFs can stimulate the proliferation of immune cells and inhibit the growth of CD4+/CD8+ T cells. However, after ZSP4 stimulation, the functions of prostate CAFs were inhibited. The mechanism experiment shows that ZSP4 can stimulate prostate CAFs by down-regulating the expression of α-smooth muscle actin...
October 8, 2017: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/28990150/biomarkers-of-colorectal-cancer-risk-decrease-6%C3%A2-months-after-roux-en-y-gastric-bypass-surgery
#9
Sorena Afshar, Fiona Malcomson, Seamus B Kelly, Keith Seymour, Sean Woodcock, John C Mathers
PURPOSE: The impact of weight loss on obesity-related colorectal cancer (CRC) risk is not well defined. Previous studies have suggested that Roux-en-Y gastric bypass (RYGB) surgery may have an unexpected adverse impact on CRC risk. This study aimed to investigate the impact of RYGB on biomarkers of CRC risk. MATERIALS AND METHODS: Rectal mucosal biopsies and blood were obtained from patients undergoing RYGB (n = 22) and non-obese control participants (n = 20) at baseline and at a median of 6...
October 8, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/28990111/microrna%C3%A2-720-inhibits-pancreatic-cancer-cell-proliferation-and-invasion-by-directly-targeting-cyclin-d1
#10
Yu Zhang, Yueying Su, Yabing Zhao, Guoqiang Lv, Ying Luo
Pancreatic cancer is the fourth leading cause of cancer‑associated deaths in Western countries, and ranks sixth among cancer‑associated diseases, with the highest mortality rate in China. Deregulation of micro (miR) RNA may contribute to the occurrence and progression of numerous cancers, including pancreatic cancer. In particular, deregulation of microRNA‑720 (miR‑720) has been reported in various types of human cancer. However, the expression and biological role of miR‑720 in pancreatic cancer remains to be elucidated...
October 5, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28990105/microrna%C3%A2-24%C3%A2-2-is-associated-with-cell-proliferation-invasion-migration-and-apoptosis-in-renal-cell-carcinoma
#11
Lu Jin, Yifan Li, Liping Nie, Tao He, Jia Hu, Jiaju Liu, Mingwei Chen, Min Shi, Zhimao Jiang, Yaoting Gui, Shangqi Yang, Yongqing Lai
Micro (mi)RNAs are involved in multiple cellular processes, and alterations in miRNA expression have been demonstrated to lead to tumorigenesis. Previous microarray analysis revealed that miRNA (miR)‑24 was downregulated in renal cell carcinoma (RCC). Additionally, miR‑24 has been identified as an oncogene and tumor suppressor in various cancers. The present study assessed the expression levels of two stem‑loops of miR‑24, miR‑24‑1 and miR‑24‑2, in RCC tissues and paired healthy tissues by reverse transcription‑quantitative polymerase chain reaction...
October 4, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28990088/identification-and-bioinformatics-analysis-of-mirnas-associated-with-human-muscle-invasive-bladder-cancer
#12
Liang Chen, Lushun Yuan, Gang Wang, Rui Cao, Jianping Peng, Bo Shu, Guofeng Qian, Xinghuan Wang, Yu Xiao
Accumulated evidence has indicated that micro (mi)RNAs play vital roles in the occurrence and development of human muscle invasive bladder cancer (MIBC), however, little is known about the miRNAs' regulatory networks. In the present study, the authors aimed to use bioinformatics analysis to identify the key miRNAs and potential target genes, as well as studying the underlying mechanisms for MIBC. They collected several human MIBC tissues to generate a miRNA expression analysis by microarray analysis comparing with normal bladder tissues, identifying 104 differentially expressed miRNAs (102 were downregulated and 2 were upregulated) and predicted 11,884 putative target genes of the dysregulated miRNAs...
October 4, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28988960/pdim-and-sl1-accumulation-in-mycobacterium-tuberculosis-is-associated-with-mce4a-expression
#13
Pooja Singh, Rajesh Sinha, Gaurav Tyagi, Naresh Kumar Sharma, Neeraj K Saini, Amita Chandolia, Ashok Kumar Prasad, Mandira Varma-Basil, Mridula Bose
Lipid metabolism forms the heart and soul of Mycobacterium tuberculosis life cycle. Starting from macrophage invasion at cholesterol rich micro-domains to a sustainable survival for infection by utilizing cholesterol, Mycobacterium displays the nexus of metabolic pathways around host derived lipids. mce4 operon acts as cholesterol import system in M. tuberculosis and here we demonstrate role of mce4A gene of this operon in cholesterol catabolism. Here M. tuberculosis H37Rv overexpressing Rv3499c (mce4A) recombinant was used as a model to decipher the metabolic flux during intake and utilization of host lipids by mycobacteria...
October 5, 2017: Gene
https://www.readbyqxmd.com/read/28987542/establishment-of-twist-1-and-tgfbr2-as-direct-targets-of-microrna-20a-in-mesenchymal-to-epithelial-transition-of-breast-cancer-cell-line-mda-mb-231
#14
Soumasree De, Sayantani Das, Srimoyee Mukherjee, Sainy Das, Sumita Sengupta Bandyopadhyay
Messenchymal to epithelial transition (MET) is a significant physiological phenomenon involved in embryogenesis and cancer. This study aims at investigating the mechanism of microRNA-20a (miR-20a) mediated regulation of mesenchymal to epithelial transition and identification of its direct target genes in breast cancer cell-line, MDA-MB-231. Reduced migratory and invasive property, altered cellular morphology along with reduced capability for attachment to basement membrane was acquired by over-expression of miR-20a in invasive MDA-MB-231 cell-line initially expressing low level of this micro-RNA, indicating direct correlation between abundance of miR-20a and metastatic property...
October 4, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28986833/expression-and-x-ray-structural-determination-of-the-nucleoprotein-of-lassa-fever-virus
#15
Xiaoxuan Qi, Wenjian Wang, Haohao Dong, Yuying Liang, Changjiang Dong, Hinh Ly
We describe methods to express the nucleoprotein (NP) of Lassa fever virus (LASV) in E. coli, to purify and crystallize it using the sitting-drop vapor diffusion method. The crystals were screened using Rigaku micro-007 X-ray generator and a dataset was collected at a resolution of 2.36 Å. The crystals belong to space group P3, with the unit cell parameters a = b = 176.35 Å, c = 56.40 Å, α = β = 90°, and γ = 120°. Using the X-ray diffraction method, we constructed a three-dimensional structure of the LASV NP that should aid in the development of novel therapeutic strategies against this virus, for which vaccine and effective treatment modalities are currently unavailable...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28983612/screening-and-validation-of-serum-protein-biomarkers-for-early-postmenopausal-osteoporosis-diagnosis
#16
Long Wang, Ya-Qian Hu, Zhuo-Jie Zhao, Hong-Yang Zhang, Bo Gao, Wei-Guang Lu, Xiao-Long Xu, Xi-Sheng Lin, Jin-Peng Wang, Qiang Jie, Zhuo-Jing Luo, Liu Yang
Postmenopausal osteoporosis is one of the most prominent worldwide public health problems and the morbidity is increasing with the aging population. It has been demonstrated that early diagnosis and intervention delay the disease progression and improve the outcome. Therefore, searching for biomarkers that are able to identify postmenopausal women at high risk for developing osteoporosis is an effective way to improve the quality of life of patients, and alleviate social and economic burdens. In the present study, a protein array was used to identify potential biomarkers...
September 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28982860/correlation-between-expression-of-twist-and-podoplanin-in-ductal-breast-carcinoma
#17
Jedrzej Grzegrzolka, Patrycja Wojtyra, Martyna Biala, Aleksandra Piotrowska, Agnieszka Gomulkiewicz, Janusz Rys, Marzenna Podhorska-Okolow, Piotr Dziegiel
BACKGROUND/AIM: As a result of activation of transcription factors engaged in epithelial-mesenchymal transition (EMT), such as Twist, inhibition of epithelial markers and an increased expression of mesenchymal markers are observed. One of the specific markers of cancer-associated fibroblasts is podoplanin (PDPN) - a mucin-type membrane glycoprotein. The aim of this work was to study the localisation and intensity of expression of Twist and PDPN on the mRNA and protein level in cases of invasive ductal breast carcinoma (IDC), and its association with patients' clinico-pathological data...
October 2017: Anticancer Research
https://www.readbyqxmd.com/read/28982680/chronic-psychosocial-stress-disturbs-long-bone-growth-in-adolescent-mice
#18
S Foertsch, M Haffner-Luntzer, J Kroner, F Gross, K Kaiser, M Erber, S O Reber, A Ignatius
Although a strong association between psychiatric and somatic disorders is generally accepted, little is known regarding the interrelation between mental and skeletal health. While depressive disorders were shown to be strongly associated with osteoporosis and increased fracture risk, evidence from post-traumatic stress disorder (PTSD) patients is less consistent. Therefore, the present study investigated the influence of chronic psychosocial stress on bone using a well-established murine model for PTSD. 7-week-old C57BL/6N mice were subjected to chronic subordinate colony housing (CSC) for 19 days, whereas control mice were singly housed...
October 5, 2017: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/28978048/micro-rna-155-plays-a-critical-role-in-the-initiation-of-food-allergen-related-inflammation-in-the-intestine
#19
Ri-Tian Lin, Jiang-Qi Liu, Hui-Ying Lu, Ya-Mei Chen, Li Guan, Zhi-Gang Liu, Zhan-Ju Liu, Ping-Chang Yang
The pathogenesis of food allergy (FA) is to be further investigated. Regulatory B cells (B10 cell) play a critical in the maintenance of the homeostasis in the intestine. Deregulation of B10 cell is associated with immune inflammation. Micro RNA (miR) 155 is involved in affecting immune cell function. This study tests a hypothesis that miR-155 affects the B10 cell function to facilitate the initiation of FA. In this study, BALB/c mice were sensitized to ovalbumin (OVA) to induce FA-like inflammation in the intestine...
September 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28976615/establishment-of-breast-tumor-spheroids-an-emerging-research-tool
#20
Azza Mohamed Akasha, J Julie Kim
Breast cancer research has historically relied on two model systems: two-dimensional cell culture systems and mouse models. These models, although very convenient, lack the functional phenotypic architecture of human tissue. Extrapolation of hormonal responses from models to human relevance also requires further investigation due to the anatomical, physiological, and biological differences. Three-dimensional spheroids provide alternative models as they better represent the architecture and maintain the histomorphological, functional, and micro-environmental features of the in vivo human tumor tissue...
October 4, 2017: Molecular Reproduction and Development
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