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https://www.readbyqxmd.com/read/29679684/expression-profile-and-reproductive-regulation-of-apgwamide-in-pacific-abalone-haliotis-discus-hannai
#1
Kyeong Seop Kim, Tae Ha Kim, Mi Ae Kim, Jung Sick Lee, Young Chang Sohn
Neuropeptides in the central nervous system regulate reproductive activities in vertebrates. Ala-Pro-Gly-Trp-NH2 (APGWamide), a neuromediator expressed in the neural ganglia of mollusks, controls sexual maturation and reproduction. To clarify the role of APGWamide in sexual behavior regulation and gamete cell maturation in mollusks, we cloned the cDNA of APGWamide precursor (Hdh-APGWamide) and examined the spatiotemporal expression of the transcript in the Pacific abalone Haliotis discus hannai. The 222-amino acid sequence of the precursor deduced from the cDNA sequence showed typical features of gastropod APGWamide precursors...
April 18, 2018: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/29674376/cherchez-la-femme-impact-of-ocean-acidification-on-the-egg-jelly-coat-and-attractants-for-sperm
#2
Shawna A Foo, Dione Deaker, Maria Byrne
The impact of ocean acidification on marine invertebrate eggs and consequences for sperm chemotaxis are unknown. In the sea urchins Heliocidaris tuberculata and H. erythrogramma , with small (93µm) and large (393µm) eggs, respectively, we documented the effect of decreased pH on the egg jelly coat, an extracellular matrix that increases target size for sperm and contains sperm attracting molecules. In near future conditions (pH 7.8, 7.6) the jelly coat of H. tuberculata decreased by 11 and 21%, reducing egg target size by 9 and 17%, respectively...
April 19, 2018: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29673874/origins-of-lifetime-health-around-the-time-of-conception-causes-and-consequences
#3
REVIEW
Tom P Fleming, Adam J Watkins, Miguel A Velazquez, John C Mathers, Andrew M Prentice, Judith Stephenson, Mary Barker, Richard Saffery, Chittaranjan S Yajnik, Judith J Eckert, Mark A Hanson, Terrence Forrester, Peter D Gluckman, Keith M Godfrey
Parental environmental factors, including diet, body composition, metabolism, and stress, affect the health and chronic disease risk of people throughout their lives, as captured in the Developmental Origins of Health and Disease concept. Research across the epidemiological, clinical, and basic science fields has identified the period around conception as being crucial for the processes mediating parental influences on the health of the next generation. During this time, from the maturation of gametes through to early embryonic development, parental lifestyle can adversely influence long-term risks of offspring cardiovascular, metabolic, immune, and neurological morbidities, often termed developmental programming...
April 16, 2018: Lancet
https://www.readbyqxmd.com/read/29670338/preference-for-anonymity-in-sperm-donation-for-artificial-insemination-an-experience-from-low-resource-settings-in-nigeria
#4
Euzebus Chinonye Ezugwu, George U Eleje, Chukwuemeka A Iyoke, Sunday G Mba, Henry C Nnaji, Chukwunonso I Enechukwu, Peter O Nkwo
Objective: Anonymous sperm donation is a common practice in Nigeria with its associated legal and ethical challenges. This study aimed to investigate infertile couples' opinions about issues of sperm donor anonymity and to determine factors that might influence their preferences. Methods: A cross-sectional, multicentered, questionnaire-based study was conducted among infertile couples attending infertility clinics in three tertiary hospitals in the south-eastern region of Nigeria over a period of 6 months...
2018: Patient Preference and Adherence
https://www.readbyqxmd.com/read/29667547/new-markers-for-predicting-fertility-of-the-male-gametes-in-the-post-genomic-age
#5
Savina Dipresa, Luca De Toni, Carlo Foresta, Andrea Garolla
A number of test have been proposed to assess male fertility potential, ranging from routine testing by light microscopic method for evaluating semen samples, to screening test for DNA integrity aimed to look at sperm chromatin abnormalities. Spermatozoa are an extremely differentiated cell, they have critical functions for embryo development and heredity, in addiction to delivering a haploid paternal genome to the oocyte. Towards this goal certain requirements must always be met. The ability of spermatozoa to perform its reproductive function taking place in the spermatogenesis, a highly specialized process depending on multiple factors with effect on male fertility...
April 18, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29666883/snp-based-pool-genotyping-and-haplotype-analysis-accelerate-fine-mapping-of-the-wheat-genomic-region-containing-stripe-rust-resistance-gene-yr26
#6
Jianhui Wu, Qingdong Zeng, Qilin Wang, Shengjie Liu, Shizhou Yu, Jingmei Mu, Shuo Huang, Hanan Sela, Assaf Distelfeld, Lili Huang, Dejun Han, Zhensheng Kang
NGS-assisted super pooling emerging as powerful tool to accelerate gene mapping and haplotype association analysis within target region uncovering specific linkage SNPs or alleles for marker-assisted gene pyramiding. Conventional gene mapping methods to identify genes associated with important agronomic traits require significant amounts of financial support and time. Here, a single nucleotide polymorphism (SNP)-based mapping approach, RNA-Seq and SNP array assisted super pooling analysis, was used for rapid mining of a candidate genomic region for stripe rust resistance gene Yr26 that has been widely used in wheat breeding programs in China...
April 17, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29666339/phenotypic-characterization-of-derivative-22-syndrome-case-series-and-review
#7
Deepti Saxena, Priyanka Srivastava, Moni Tuteja, Kausik Mandal, Shubha R Phadke
Emanuel syndrome is caused due to an additional derivative chromosome 22 and is characterized by severe intellectual disability, microcephaly, failure to thrive, preauricular tags or pits, ear anomalies, cleft or high-arched palate, micrognathia, kidney abnormalities, congenital heart defects and genital abnormalities in males. In 99% of the cases, one of the parents is a carrier of balanced translocation between chromosomes 11 and 22. It occurs due to malsegregation of the gametes with 3:1 segregation. In this case series, we describe four patients with diverse manifestations of this condition...
March 2018: Journal of Genetics
https://www.readbyqxmd.com/read/29663591/research-progress-on-the-role-of-melatonin-and-its-receptors-in-animal-reproduction-a-comprehensive-review
#8
REVIEW
H S Talpur, I B Chandio, R D Brohi, T Worku, Z Rehman, D Bhattarai, F Ullah, L JiaJia, L Yang
Melatonin and its receptors play a crucial role in the regulation of the animal reproductive process, primarily in follicular development. However, the role that melatonin performs in regulating hormones related with reproduction remains unclear. Melatonin and its receptors are present both in female and male animals' organs, such as ovaries, heart, brain and liver. Melatonin regulates ovarian actions and is a key mediator of reproductive actions. Melatonin has numerous effects on animal reproduction, such as protection of gametes and embryos, response to clock genes, immune-neuroendocrine, reconciliation of seasonal variations in immune function, and silence or blockage of genes...
April 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29662819/variable-localization-of-toll-like-receptors-in-human-fallopian-tube-epithelial-cells
#9
Fatemehsadat Amjadi, Zahra Zandieh, Ensieh Salehi, Reza Jafari, Nasrin Ghasemi, Abbas Aflatoonian, Alireza Fazeli, Reza Aflatoonian
Objective: To determine the localization, expression, and function of Toll-like receptors (TLRs) in fallopian tube epithelial cells. Methods: The localization of TLRs in fallopian tube epithelial cells was investigated by immunostaining. Surprisingly, the intensity of staining was not equal in the secretory and ciliated cells. After primary cell culture of fallopian tube epithelial cells, ring cloning was used to isolate colonies of ciliated epithelial cells, distinct from non-ciliated epithelial cells...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662495/comparative-analysis-of-wuschel-related-homeobox-genes-revealed-their-parent-of-origin-and-cell-type-specific-expression-pattern-during-early-embryogenesis-in-tobacco
#10
Xuemei Zhou, Yingying Guo, Peng Zhao, Meng-Xiang Sun
WUSCHEL-related homeobox (WOX) gene is a plant-specific clade of homeobox transcription factors. Increasing evidences reveal that WOXs play critical roles in early embryogenesis, which involves zygote development, initiation of zygote division, and apical or basal cell lineage establishment. However, how WOXs regulate these developmental events remains largely unknown, and even detailed expression pattern in gametes and early proembryos is not yet available. Here, 13 WOX family genes were identified in Nicotiana tabacum genome...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29661137/1000-human-genomes-carry-widespread-signatures-of-gc-biased-gene-conversion
#11
Rajib Dutta, Arnab Saha-Mandal, Xi Cheng, Shuhao Qiu, Jasmine Serpen, Larisa Fedorova, Alexei Fedorov
BACKGROUND: GC-Biased Gene Conversion (gBGC) is one of the important theories put forward to explain profound long-range non-randomness in nucleotide compositions along mammalian chromosomes. Nucleotide changes due to gBGC are hard to distinguish from regular mutations. Here, we present an algorithm for analysis of millions of known SNPs that detects a subset of so-called "SNP flip-over" events representing recent gBGC nucleotide changes, which occurred in previous generations via non-crossover meiotic recombination...
April 16, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29658434/bone-marrow-derived-cells-trafficking-to-the-oviduct-effect-of-ischemia-reperfusion-injury
#12
Cagdas Sahin, Ramanaiah Mamillapalli, Hugh S Taylor
The oviduct/fallopian tube is a crucial organ in the mammalian reproductive tract; it plays a critical role in gamete transportation and early embryo development. In women, torsion of the fallopian tubes can cause ischemia and reperfusion (IR) injury. In this study, we tested the effect of this injury on recruitment of bone marrow-derived cells (BMDCs) to the oviducts of reproductive age female mice. Bone marrow-derived cells were collected from ubiquitin-green fluorescent protein-positive male mice and transplanted into wild-type female mice...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29657233/reproductive-technologies-for-the-generation-and-maintenance-of-valuable-animal-strains
#13
Takehito Kaneko
Many types of mutant and genetically engineered strains have been produced in various animal species. Their numbers have dramatically increased in recent years, with new strains being rapidly produced using genome editing techniques. In the rat, it has been difficult to produce knockout and knock-in strains because the establishment of stem cells has been insufficient. However, a large number of knockout and knock-in strains can currently be produced using genome editing techniques, including zinc-finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) system...
April 14, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29656830/cairo-consensus-on-the-ivf-laboratory-environment-and-air-quality-report-of-an-expert-meeting
#14
D Mortimer, J Cohen, S T Mortimer, M Fawzy, D H McCulloh, D E Morbeck, X Pollet-Villard, R T Mansour, D R Brison, A Doshi, J C Harper, J E Swain, A V Gilligan
This proceedings report presents the outcomes from an international Expert Meeting to establish a consensus on the recommended technical and operational requirements for air quality within modern assisted reproduction technology (ART) laboratories. Topics considered included design and construction of the facility, as well as its heating, ventilation and air conditioning system; control of particulates, micro-organisms (bacteria, fungi and viruses) and volatile organic compounds (VOCs) within critical areas; safe cleaning practices; operational practices to optimize air quality while minimizing physicochemical risks to gametes and embryos (temperature control versus air flow); and appropriate infection-control practices that minimize exposure to VOC...
March 2, 2018: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29656282/intrapopulation-chromosomal-polymorphism-in-mazama-gouazoubira-cetartiodactyla-cervidae-the-emergence-of-a-new-species
#15
Mirela P Valeri, Iara M Tomazella, José M B Duarte
Mazama gouazoubira is a small deer species widely distributed in South America. Previous studies have shown that this species presents intraspecific chromosomal polymorphisms, which could affect fertility due to the effects of chromosomal rearrangements on gamete formation. Important aspects regarding the karyotype evolution of this species and the genus remain undefined due to the lack of information concerning the causes of this chromosomal variation. Nineteen individuals belonging to the Mazama gouazoubira population located in the Pantanal were cytogenetically evaluated...
April 14, 2018: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/29651940/proteomics-in-human-assisted-reproduction
#16
Mauro Bibancos De Rose, Mariana Moraes Piccolomini
Assisted reproduction techniques (ART) have significantly advanced since the first successful in vitro fertilization (IVF). However, most in vitro-produced embryos fail to implant. Key steps in ART are the correct infertility diagnosis, in order to manage the individual treatments, and the assessment of gamete and embryo viability, to identify the embryo with the best implantation potential. Non-invasive approaches to correctly diagnose infertility and to access embryo development potential have the advantages of increasing the knowledge of embryo physiology, therefore allowing the development of methods to predict developmental competence and viability...
April 12, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29651936/a-step-by-step-protocol-for-mammalian-sperm-protamine-extraction-and-analysis
#17
Ada Soler-Ventura, Judit Castillo, Alberto de la Iglesia, Meritxell Jodar, Ferran Barrachina, Josep Lluis Ballesca, Rafael Oliva
Protamines are the most abundant sperm nuclear proteins and pack approximately the 92-98% of the mammalian sperm DNA. In mammals, two types of protamines have been described, the protamine 1 (P1) and the protamine 2 (P2) family, whose relative ratio has been related to the proper sperm chromatin maturity and the normality/abnormality of the male gamete. Specifically, the deregulation of the P1/P2 ratio has been correlated to DNA damage, alterations in seminal parameters, and low success rate of assisted reproduction techniques...
April 12, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29649725/production-of-live-fish-derived-from-frozen-germ-cells-via-germ-cell-transplantation
#18
Goro Yoshizaki, Seungki Lee
The conservation of endangered fish is an urgent issue. Although cryo-banking of fish gametes might ultimately help conserve endangered fish, cryopreservation of fish eggs or embryos is still not possible due to their large size and high yolk content. Therefore, as an alternative, we focused on undifferentiated germ cells, such as primordial germ cells, spermatogonia, and oogonia, as materials for cryopreservation. Transplantation of cryopreserved germ cells into the body cavity of allogeneic or xenogeneic recipients sterilized by triploidization or endogenous germ cell ablation resulted in migration of the transplanted germ cells toward the recipient genital ridges, where they were eventually incorporated...
April 3, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29649254/uxt-is-required-for-spermatogenesis-in-mice
#19
Eric D Schafler, Phillip A Thomas, Susan Ha, Yu Wang, Keria Bermudez-Hernandez, Zuojian Tang, David Fenyö, Margarita Vigodner, Susan K Logan
Male mammals must simultaneously produce prodigious numbers of sperm and maintain an adequate reserve of stem cells to ensure continuous production of gametes throughout life. Failures in the mechanisms responsible for balancing germ cell differentiation and spermatogonial stem cell (SSC) self-renewal can result in infertility. We discovered a novel requirement for Ubiquitous Expressed Transcript (UXT) in spermatogenesis by developing the first knockout mouse model for this gene. Constitutive deletion of Uxt is embryonic lethal, while conditional knockout in the male germline results in a Sertoli cell-only phenotype during the first wave of spermatogenesis that does not recover in the adult...
2018: PloS One
https://www.readbyqxmd.com/read/29644457/an-interplay-between-shugoshin-and-spo13-for-centromeric-cohesin-protection-and-sister-kinetochore-mono-orientation-during-meiosis-i-in-saccharomyces-cerevisiae
#20
Gunjan Mehta, Guhan Kaliyaperumal Anbalagan, Akhilendra Pratap Bharati, Purna Gadre, Santanu Kumar Ghosh
Meiosis is a specialized cell division process by which haploid gametes are produced from a diploid mother cell. Reductional chromosome segregation during meiosis I (MI) is achieved by two unique and conserved events: centromeric cohesin protection (CCP) and sister kinetochore mono-orientation (SKM). In Saccharomyces cerevisiae, a meiosis-specific protein Spo13 plays a role in both these centromere-specific events. Despite genome-wide association of Spo13, we failed to detect its function in global processes such as cohesin loading, cohesion establishment and homologs pairing...
April 11, 2018: Current Genetics
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