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Surendra K Sharma, Anunay Gupta, Ashutosh Biswas, Abhishek Sharma, Atul Malhotra, K T Prasad, Sreenivas Vishnubhatla, Sajal Ajmani, Hridesh Mishra, Manish Soneja, Shobha Broor
BACKGROUND & OBJECTIVES: Acute respiratory distress syndrome (ARDS) is a common disorder in critically ill patients and is associated with high mortality. There is a paucity of literature on this condition from developing countries. This prospective observational study was designed to find out the aetiology, outcomes and predictors of mortality in ARDS. METHODS: Sixty four consecutive patients who satisfied American-European Consensus Conference (AECC) definition of ARDS from medical Intensive Care Unit (ICU) of a tertiary care centre in New Delhi, India, were enrolled in the study...
June 2016: Indian Journal of Medical Research
Deepshikha Kumar, Shobha Broor, Maitreyi S Rajala
Influenza A virus nucleoprotein, is a multifunctional RNA-binding protein, encoded by segment-5 of the negative sense RNA genome. It serves as a key connector between the virus and the host during virus replication. It continuously shuttles between the cytoplasm and the nucleus interacting with various host cellular factors. In the current study, host proteins interacting with nucleoprotein of Influenza A virus of H1N1 2009 pandemic strain were identified by co-immunoprecipitation studies followed by MALDI-TOF/MS analysis...
2016: PloS One
A Tazeen, N Afreen, M Abdullah, F Deeba, S H Haider, S N Kazim, S Ali, I H Naqvi, S Broor, A Ahmed, S Parveen
Dengue fever is an arthropod-borne viral infection that has become endemic in several parts of India including Delhi. We studied occurrence of co-infection with dengue viruses during an outbreak in New Delhi, India in 2014. For the present study, blood samples collected from symptomatic patients were analysed by RT-PCR. Eighty percent of the samples were positive for dengue virus. The result showed that DENV-1 (77%) was the predominant serotype followed by DENV-2 (60%). Concurrent infection with more than one serotype was identified in 43% of the positive samples...
September 13, 2016: Epidemiology and Infection
Nazia Afreen, Irshad H Naqvi, Shobha Broor, Anwar Ahmed, Syed Naqui Kazim, Ravins Dohare, Manoj Kumar, Shama Parveen
Dengue fever is the most important arboviral disease in the tropical and sub-tropical countries of the world. Delhi, the metropolitan capital state of India, has reported many dengue outbreaks, with the last outbreak occurring in 2013. We have recently reported predominance of dengue virus serotype 2 during 2011-2014 in Delhi. In the present study, we report molecular characterization and evolutionary analysis of dengue serotype 2 viruses which were detected in 2011-2014 in Delhi. Envelope genes of 42 DENV-2 strains were sequenced in the study...
March 2016: PLoS Neglected Tropical Diseases
Anant Mohan, Arvind Uniyal, Subhash Chandra, Molu C Ozukum, Bharti Gaur, Shobha Broor, Kriti Upadhyaya, Ashraf Ali, Ashish Upadhyaya, Randeep Guleria
No abstract text is available yet for this article.
November 2015: Lung India: Official Organ of Indian Chest Society
Farah Deeba, Asimul Islam, Syed Naqui Kazim, Irshad Hussain Naqvi, Shobha Broor, Anwar Ahmed, Shama Parveen
The Chikungunya virus is a re-emerging alphavirus that belongs to the family Togaviridae. The symptoms include fever, rashes, nausea and joint pain that may last for months. The laboratory diagnosis of the infection is based on the serologic assays, virus isolation and molecular methods. The pathogenesis of the Chikungunya viral infection is not completely understood. Some of the recent investigations have provided information on replication of the virus in various cells and organs. In addition, some recent reports have indicated that the severity of the disease is correlated with the viral load and cytokines...
April 2016: Pathogens and Disease
Siddhartha Saha, Bharti Gaur Pandey, Avinash Choudekar, Anand Krishnan, Susan I Gerber, Sanjay K Rai, Pratibha Singh, Mandeep Chadha, Renu B Lal, Shobha Broor
BACKGROUND: The burden estimation studies for respiratory syncytial virus (RSV) have been based on varied case definitions, including case-definitions designed for influenza surveillance systems. We used all medical admissions among children aged 0-59 months to study the effect of case definitions on estimation of RSV-associated hospitalizations rates. METHODS: The hospital-based daily surveillance enrolled children aged 0-59 months admitted with acute medical conditions from July 2009-December 2012, from a well-defined rural population in Ballabgarh in northern India...
December 2015: Journal of Global Health
Vln Raghuram S, Wajihul Hasan Khan, Farah Deeba, Wayne Sullender, Shobha Broor, Shama Parveen
Human respiratory syncytial virus (hRSV) is the most common viral pathogen of acute lower respiratory tract infection in infants and young children. The G protein of hRSV is the trans-membrane glycoprotein that is involved in the attachment of virion with the host cell. The nasopharyngeal aspirates were subjected to RT-PCR for the second hypervariable region of the G protein gene in the present investigation. Sequencing and phylogenetic analysis revealed that all the study strains clustered within the BA genotype...
December 2015: Virusdisease
Hitendra Garg, Subhash Gupta, A C Anand, S L Broor
Portal hypertensive gastropathy (PHG) and gastric antral vascular ectasia (GAVE) are gastric mucosal lesions that mostly present as chronic anemia and rarely cause the acute gastrointestinal hemorrhage. Despite similar clinical manifestations, their pathophysiology and management are entirely different. PHG is seen exclusively in patients with portal hypertension, but GAVE can also be observed in patients with other conditions. Their diagnosis is endoscopic, and although generally each of them has a characteristic endoscopic appearance and distribution, there are cases in which the differential is difficult and must rely on histology...
September 2015: Indian Journal of Gastroenterology: Official Journal of the Indian Society of Gastroenterology
Nazia Afreen, Irshad H Naqvi, Shobha Broor, Anwar Ahmed, Shama Parveen
Dengue fever is the most prevalent arboviral disease in the tropical and sub-tropical regions of the world. The present report describes molecular detection and serotyping of dengue viruses in acute phase blood samples collected from New Delhi, India. Phylogenetic and molecular clock analysis of dengue virus serotype 1 and 3 strains were also investigated. Dengue virus infection was detected in 68.87% out of 604 samples tested by RT-PCR between 2011 & 2014. Dengue serotype 1 was detected in 25.48% samples, dengue serotype 2 in 79...
2015: PloS One
Anand Krishnan, Ritvik Amarchand, Vivek Gupta, Kathryn E Lafond, Rizwan Abdulkader Suliankatchi, Siddhartha Saha, Sanjay Rai, Puneet Misra, Debjani Ram Purakayastha, Abhishek Wahi, Vishnubhatla Sreenivas, Arti Kapil, Fatimah Dawood, Chandrakant S Pandav, Shobha Broor, Suresh K Kapoor, Renu Lal, Marc-Alain Widdowson
BACKGROUND: Despite acute respiratory infections being a major cause of death among children in developing countries including India, there is a lack of community-based studies that document its burden and aetiology. METHODS: A dynamic cohort of children aged 0-10 years was established in four villages in a north Indian state of Haryana from August 2012 onwards. Trained health workers conducted weekly home visits to screen children for acute respiratory infection (ARI) defined as one of the following: cough, sore throat, nasal congestion, earache/discharge, or breathing difficulty...
2015: BMC Infectious Diseases
Wajihul Hasan Khan, V L N Raghuram Srungaram, Asimul Islam, Ilyas Beg, Md Shakir H Haider, Faizan Ahmad, Shobha Broor, Shama Parveen
Human respiratory syncytial virus (hRSV) is an important pathogen of acute respiratory tract infection. The G protein of hRSV is a transmembrane glycoprotein that is a neutralizing antigen and is thus a vaccine candidate. In this study, synthetic codon optimized ectodomain G protein [G(ΔTM)] of BA genotype of group B hRSV was cloned, expressed, and characterized using biophysical techniques. The molar absorption coefficient and mean residue ellipticity at 222 nm ([θ]222) of G (ΔTM) was found to be 7950 M(-1) cm(-1) and -19701...
July 3, 2016: Preparative Biochemistry & Biotechnology
Sujit K Chowdhary, Deepak K Kandpal, Deepak Agarwal, Saroja Balan, Nameet Jerath, Anupam Sibal, Sohan L Broor
AIM: The aim of the study is to report feasibility and safety of endoscopic esophageal substitution in infants with pure esophageal atresia and wide gap tracheoesophageal fistula with a minimum one year follow-up. MATERIALS AND METHODS: This prospective study was conducted from January 2012 for twenty four consecutive months at Apollo Hospital, New Delhi. All babies either followed up or referred for esophageal substitution without any history of mediastinitis or associated major congenital anomaly and weighing greater than 6kg were to be included in the study...
March 2016: Journal of Pediatric Surgery
Trevor Bedford, Steven Riley, Ian G Barr, Shobha Broor, Mandeep Chadha, Nancy J Cox, Rodney S Daniels, C Palani Gunasekaran, Aeron C Hurt, Anne Kelso, Alexander Klimov, Nicola S Lewis, Xiyan Li, John W McCauley, Takato Odagiri, Varsha Potdar, Andrew Rambaut, Yuelong Shu, Eugene Skepner, Derek J Smith, Marc A Suchard, Masato Tashiro, Dayan Wang, Xiyan Xu, Philippe Lemey, Colin A Russell
Understanding the spatiotemporal patterns of emergence and circulation of new human seasonal influenza virus variants is a key scientific and public health challenge. The global circulation patterns of influenza A/H3N2 viruses are well characterized, but the patterns of A/H1N1 and B viruses have remained largely unexplored. Here we show that the global circulation patterns of A/H1N1 (up to 2009), B/Victoria, and B/Yamagata viruses differ substantially from those of A/H3N2 viruses, on the basis of analyses of 9,604 haemagglutinin sequences of human seasonal influenza viruses from 2000 to 2012...
July 9, 2015: Nature
Mandeep S Chadha, Varsha A Potdar, Siddhartha Saha, Parvaiz A Koul, Shobha Broor, Lalit Dar, Mamta Chawla-Sarkar, Dipankar Biswas, Palani Gunasekaran, Asha Mary Abraham, Sunanda Shrikhande, Amita Jain, Balakrishnan Anukumar, Renu B Lal, Akhilesh C Mishra
BACKGROUND: Influenza surveillance is an important tool to identify emerging/reemerging strains, and defining seasonality. We describe the distinct patterns of circulating strains of the virus in different areas in India from 2009 to 2013. METHODS: Patients in ten cities presenting with influenza like illness in out-patient departments of dispensaries/hospitals and hospitalized patients with severe acute respiratory infections were enrolled. Nasopharangeal swabs were tested for influenza viruses by real-time RT-PCR, and subtyping; antigenic and genetic analysis were carried out using standard assays...
2015: PloS One
Samuel K Peasah, Debjani Ram Purakayastha, Parvaiz A Koul, Fatima S Dawood, Siddhartha Saha, Ritvik Amarchand, Shobha Broor, Vaibhab Rastogi, Romana Assad, Kaisar Ahmed Kaul, Marc-Alain Widdowson, Renu B Lal, Anand Krishnan
BACKGROUND: Despite the high mortality and morbidity resulting from acute respiratory infections (ARI) globally, there are few data from low-income countries on costs of ARI to inform public health policy decisions We conducted a prospective survey to assess costs of ARI episodes in selected primary, secondary, and tertiary healthcare facilities in north India where no respiratory pathogen vaccine is routinely recommended. METHODS: Face-to-face interviews were conducted among a purposive sample of patients with ARI from healthcare facilities...
2015: BMC Public Health
Nishat Hussain Ahmed, Shobha Broor
BACKGROUND: Dengue viruses, single-stranded positive polarity ribonucleic acid (RNA) viruses of the family Flaviviridae, are the most common cause of arboviral disease in the world. We report a clinico-epidemiological study of the dengue fever outbreak of 2010 from a tertiary care hospital in Delhi, North India. OBJECTIVES: Objectives of the study were to know the incidence of laboratory-confirmed dengue cases among the clinically suspected patients; to study the clinical profile of dengue-positive cases; and to co-relate the above with the prevalent serotype and environmental conditions...
April 2015: Indian Journal of Community Medicine
Nazia Afreen, Farah Deeba, Irshad Naqvi, Mohammad Shareef, Anwar Ahmed, Shobha Broor, Shama Parveen
Dengue fever is a self-limiting, acute febrile disease which may aggravate to haemorrhage, plasma leakage and organ impairment in small number of cases. An outbreak of dengue fever occurred in Delhi, India after rainy season in the year 2013. Dengue virus specific RT-PCR was carried out on 378 suspected blood samples that were collected during the outbreak. Dengue virus was detected in 71% samples with highest number of patients infected by DENV-2 (86%) followed by DENV-1 (19 %) and DENV-3 (8%). Co-infection with more than one DENV serotype was detected in 14% samples...
2014: PLoS Currents
S Dhakad, P C Mali, S Kaushik, A A Lal, S Broor
PURPOSE: Influenza epidemics and periodic pandemics occur worldwide resulting in significant mortality, morbidity and economic loss. There is need for a sensitive, rapid and cost-effective assay to detect, type and sub-type influenza viruses, as cell culture has a long turnaround time. MATERIALS AND METHODS: Nasopharyngeal swabs were collected from patients presenting with influenza-like illness (ILI) at AIIMS OPD and Primary Health Centre Ballabhgarh (Haryana)...
January 2015: Indian Journal of Medical Microbiology
Parvaiz A Koul, Umar H Khan, Romana Asad, Rubaya Yousuf, Shobha Broor, Renu B Lal, Fatimah S Dawood
We estimate the contribution of influenza to hospitalizations for acute exacerbations of chronic obstructive pulmonary disease (AECOPD) in Kashmir, India. Prospective surveillance for influenza among patients hospitalized with AECOPD was conducted at a tertiary care hospital. Patients had clinical data collected and nasal/throat swabs tested for influenza viruses. Outcomes among patients with and without influenza were compared with logistic regression adjusting for age and underlying conditions. During October 2010-September 2012, 498 patients hospitalized with AECOPD were enrolled, of whom 40 (8%) had received influenza vaccine...
January 2015: Influenza and Other Respiratory Viruses
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