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https://www.readbyqxmd.com/read/29735822/molecular-characterisation-of-staphylococcus-aureus-using-spa-typing-as-a-diagnostic-tool-in-haryana-india
#1
Gurjeet Singh, Shobha Broor, Priti Agarwal
Background: Staphylococcus aureus is one of the top six most common etiologic agents of nosocomial, community and livestock acquired bacterial infections. These infections although initially were described as a major problem in hospitals have now also become a serious threat in community not only in India but also worldwide. Its prevalence varies depending on the health-care setting, country or a particular region. Thus to better understand the epidemiology of methicillin-resistant S...
January 2018: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29698505/estimation-of-community-level-influenza-associated-illness-in-a-low-resource-rural-setting-in-india
#2
Siddhartha Saha, Vivek Gupta, Fatimah S Dawood, Shobha Broor, Kathryn E Lafond, Mandeep S Chadha, Sanjay K Rai, Anand Krishnan
OBJECTIVE: To estimate rates of community-level influenza-like-illness (ILI) and influenza-associated ILI in rural north India. METHODS: During 2011, we conducted household-based healthcare utilization surveys (HUS) for any acute medical illness (AMI) in preceding 14days among residents of 28villages of Ballabgarh, in north India. Concurrently, we conducted clinic-based surveillance (CBS) in the area for AMI episodes with illness onset ≤3days and collected nasal and throat swabs for influenza virus testing using real-time polymerase chain reaction...
2018: PloS One
https://www.readbyqxmd.com/read/29694383/ba9-lineage-of-respiratory-syncytial-virus-from-across-the-globe-and-its-evolutionary-dynamics
#3
Md Shakir Hussain Haider, Wajihul Hasan Khan, Farah Deeba, Sher Ali, Anwar Ahmed, Irshad H Naqvi, Ravins Dohare, Hytham A Alsenaidy, Abdulrahman M Alsenaidy, Shobha Broor, Shama Parveen
Respiratory syncytial virus (RSV) is an important pathogen of global significance. The BA9 is one of the most predominant lineages of the BA genotype of group B RSV that has acquired a 60bp duplication in its G protein gene. We describe the local and global evolutionary dynamics of the second hyper variable region in the C- terminal of the G protein gene of the BA9 lineage. A total of 418 sequences (including 31 study and 387 GenBank strains) from 29 different countries were used for phylogenetic analysis. This analysis showed that the study strains clustered with BA (BA9 and BA8) and SAB4 genotype of group B RSV...
2018: PloS One
https://www.readbyqxmd.com/read/29427763/global-distribution-of-na1-genotype-of-respiratory-syncytial-virus-and-its-evolutionary-dynamics-assessed-from-the-past-11-years
#4
Md Shakir Hussain Haider, Farah Deeba, Wajihul Hasan Khan, Irshad H Naqvi, Sher Ali, Anwar Ahmed, Shobha Broor, Hytham A Alsenaidy, Abdulrahman M Alsenaidy, Ravins Dohare, Shama Parveen
Respiratory syncytial virus (RSV) is a potent pathogen having global distribution. The main purpose of this study was to gain an insight into distribution pattern of the NA1 genotype of group A RSV across the globe together with its evolutionary dynamics. We focused on the second hypervariable region of the G protein gene and used the same for Phylogenetic, Bayesian and Network analyses. Eighteen percent of the samples collected from 500 symptomatic pediatric patients with acute respiratory tract infection (ARI) were found to be positive for RSV during 2011-15 from New Delhi, India...
June 2018: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29112737/estimation-of-burden-of-influenza-among-under-five-children-in-india-a-meta-analysis
#5
Debjani Ram Purakayastha, Sreenivas Vishnubhatla, Sanjay Kumar Rai, Sobha Broor, Anand Krishnan
Background: We estimated the burden of influenza-related acute respiratory tract infection (ARI) among under-fives in India through meta-analysis. Methodology: We estimated pooled incidence and proportional positivity of laboratory-diagnosed influenza among under-fives using data from observational studies published from 1 January 1961 to 31 December 2016. Death due to influenza was estimated using a multiplier model. Results: Influenza-associated ARI incidence was estimated as 132 per 1000 child-years (115-149)...
November 3, 2017: Journal of Tropical Pediatrics
https://www.readbyqxmd.com/read/28831731/cost-of-treatment-of-febrile-acute-respiratory-infection-fari-among-under-five-children-attending-health-facilities-of-ballabgarh-haryana
#6
Debjani Ram Purakayastha, Sanjay Kumar Rai, Shobha Broor, Anand Krishnan
OBJECTIVE: To estimate the expenditure incurred towards treatment of an episode of respiratory infection among under-fives in outpatient and inpatient departments of primary and secondary level health facilities. METHODS: During March 2011 - September 2012, under-five children presenting with febrile acute respiratory infection (FARI) in the outpatient (OPD) and inpatient (IPD) departments of public and private health facilities of Ballabgarh, Haryana were enrolled in the study...
December 2017: Indian Journal of Pediatrics
https://www.readbyqxmd.com/read/28689664/global-regional-and-national-disease-burden-estimates-of-acute-lower-respiratory-infections-due-to-respiratory-syncytial-virus-in-young-children-in-2015-a-systematic-review-and-modelling-study
#7
Ting Shi, David A McAllister, Katherine L O'Brien, Eric A F Simoes, Shabir A Madhi, Bradford D Gessner, Fernando P Polack, Evelyn Balsells, Sozinho Acacio, Claudia Aguayo, Issifou Alassani, Asad Ali, Martin Antonio, Shally Awasthi, Juliet O Awori, Eduardo Azziz-Baumgartner, Henry C Baggett, Vicky L Baillie, Angel Balmaseda, Alfredo Barahona, Sudha Basnet, Quique Bassat, Wilma Basualdo, Godfrey Bigogo, Louis Bont, Robert F Breiman, W Abdullah Brooks, Shobha Broor, Nigel Bruce, Dana Bruden, Philippe Buchy, Stuart Campbell, Phyllis Carosone-Link, Mandeep Chadha, James Chipeta, Monidarin Chou, Wilfrido Clara, Cheryl Cohen, Elizabeth de Cuellar, Duc-Anh Dang, Budragchaagiin Dash-Yandag, Maria Deloria-Knoll, Mukesh Dherani, Tekchheng Eap, Bernard E Ebruke, Marcela Echavarria, Carla Cecília de Freitas Lázaro Emediato, Rodrigo A Fasce, Daniel R Feikin, Luzhao Feng, Angela Gentile, Aubree Gordon, Doli Goswami, Sophie Goyet, Michelle Groome, Natasha Halasa, Siddhivinayak Hirve, Nusrat Homaira, Stephen R C Howie, Jorge Jara, Imane Jroundi, Cissy B Kartasasmita, Najwa Khuri-Bulos, Karen L Kotloff, Anand Krishnan, Romina Libster, Olga Lopez, Marilla G Lucero, Florencia Lucion, Socorro P Lupisan, Debora N Marcone, John P McCracken, Mario Mejia, Jennifer C Moisi, Joel M Montgomery, David P Moore, Cinta Moraleda, Jocelyn Moyes, Patrick Munywoki, Kuswandewi Mutyara, Mark P Nicol, D James Nokes, Pagbajabyn Nymadawa, Maria Tereza da Costa Oliveira, Histoshi Oshitani, Nitin Pandey, Gláucia Paranhos-Baccalà, Lia N Phillips, Valentina Sanchez Picot, Mustafizur Rahman, Mala Rakoto-Andrianarivelo, Zeba A Rasmussen, Barbara A Rath, Annick Robinson, Candice Romero, Graciela Russomando, Vahid Salimi, Pongpun Sawatwong, Nienke Scheltema, Brunhilde Schweiger, J Anthony G Scott, Phil Seidenberg, Kunling Shen, Rosalyn Singleton, Viviana Sotomayor, Tor A Strand, Agustinus Sutanto, Mariam Sylla, Milagritos D Tapia, Somsak Thamthitiwat, Elizabeth D Thomas, Rafal Tokarz, Claudia Turner, Marietjie Venter, Sunthareeya Waicharoen, Jianwei Wang, Wanitda Watthanaworawit, Lay-Myint Yoshida, Hongjie Yu, Heather J Zar, Harry Campbell, Harish Nair
BACKGROUND: We have previously estimated that respiratory syncytial virus (RSV) was associated with 22% of all episodes of (severe) acute lower respiratory infection (ALRI) resulting in 55 000 to 199 000 deaths in children younger than 5 years in 2005. In the past 5 years, major research activity on RSV has yielded substantial new data from developing countries. With a considerably expanded dataset from a large international collaboration, we aimed to estimate the global incidence, hospital admission rate, and mortality from RSV-ALRI episodes in young children in 2015...
September 2, 2017: Lancet
https://www.readbyqxmd.com/read/27859690/prevalence-and-distribution-of-chlamydia-trachomatis-genovars-in-indian-infertile-patients-a-pilot-study
#8
Jyoti Rawre, Benu Dhawan, Neena Malhotra, Vishnubhatla Sreenivas, Shobha Broor, Rama Chaudhry
To determine the prevalence and distribution of Chlamydia trachomatis genovars in patients with infertility by PCR-RFLP and ompA gene sequencing. Prevalence of other etiological agents (viz., Ureaplasma spp. and Mycoplasma hominis) were also assessed. Endocervical swabs were collected from 477 women and urine was collected from 151 men attending the Infertility Clinic. The samples were screened for C. trachomatis by cryptic plasmid, ompA gene and nested ompA gene PCR. Genotyping was performed by PCR-RFLP and sequencing...
December 2016: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/27748303/aetiology-outcomes-predictors-of-mortality-in-acute-respiratory-distress-syndrome-from-a-tertiary-care-centre-in-north-india
#9
Surendra K Sharma, Anunay Gupta, Ashutosh Biswas, Abhishek Sharma, Atul Malhotra, K T Prasad, Sreenivas Vishnubhatla, Sajal Ajmani, Hridesh Mishra, Manish Soneja, Shobha Broor
BACKGROUND & OBJECTIVES: Acute respiratory distress syndrome (ARDS) is a common disorder in critically ill patients and is associated with high mortality. There is a paucity of literature on this condition from developing countries. This prospective observational study was designed to find out the aetiology, outcomes and predictors of mortality in ARDS. METHODS: Sixty four consecutive patients who satisfied American-European Consensus Conference (AECC) definition of ARDS from medical Intensive Care Unit (ICU) of a tertiary care centre in New Delhi, India, were enrolled in the study...
June 2016: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/27711134/interaction-of-host-nucleolin-with-influenza-a-virus-nucleoprotein-in-the-early-phase-of-infection-limits-the-late-viral-gene-expression
#10
Deepshikha Kumar, Shobha Broor, Maitreyi S Rajala
Influenza A virus nucleoprotein, is a multifunctional RNA-binding protein, encoded by segment-5 of the negative sense RNA genome. It serves as a key connector between the virus and the host during virus replication. It continuously shuttles between the cytoplasm and the nucleus interacting with various host cellular factors. In the current study, host proteins interacting with nucleoprotein of Influenza A virus of H1N1 2009 pandemic strain were identified by co-immunoprecipitation studies followed by MALDI-TOF/MS analysis...
2016: PloS One
https://www.readbyqxmd.com/read/27620341/occurrence-of-co-infection-with-dengue-viruses-during-2014-in-new-delhi-india
#11
A Tazeen, N Afreen, M Abdullah, F Deeba, S H Haider, S N Kazim, S Ali, I H Naqvi, S Broor, A Ahmed, S Parveen
Dengue fever is an arthropod-borne viral infection that has become endemic in several parts of India including Delhi. We studied occurrence of co-infection with dengue viruses during an outbreak in New Delhi, India in 2014. For the present study, blood samples collected from symptomatic patients were analysed by RT-PCR. Eighty percent of the samples were positive for dengue virus. The result showed that DENV-1 (77%) was the predominant serotype followed by DENV-2 (60%). Concurrent infection with more than one serotype was identified in 43% of the positive samples...
January 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/26977703/evolutionary-analysis-of-dengue-serotype-2-viruses-using-phylogenetic-and-bayesian-methods-from-new-delhi-india
#12
Nazia Afreen, Irshad H Naqvi, Shobha Broor, Anwar Ahmed, Syed Naqui Kazim, Ravins Dohare, Manoj Kumar, Shama Parveen
Dengue fever is the most important arboviral disease in the tropical and sub-tropical countries of the world. Delhi, the metropolitan capital state of India, has reported many dengue outbreaks, with the last outbreak occurring in 2013. We have recently reported predominance of dengue virus serotype 2 during 2011-2014 in Delhi. In the present study, we report molecular characterization and evolutionary analysis of dengue serotype 2 viruses which were detected in 2011-2014 in Delhi. Envelope genes of 42 DENV-2 strains were sequenced in the study...
March 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/26664190/significance-of-viral-infections-detected-by-reverse-transcriptase-multiplex-pcr-on-hospital-related-outcomes-in-acute-exacerbations-of-chronic-obstructive-pulmonary-disease
#13
Anant Mohan, Arvind Uniyal, Subhash Chandra, Molu C Ozukum, Bharti Gaur, Shobha Broor, Kriti Upadhyaya, Ashraf Ali, Ashish Upadhyaya, Randeep Guleria
No abstract text is available yet for this article.
November 2015: Lung India: Official Organ of Indian Chest Society
https://www.readbyqxmd.com/read/26657109/chikungunya-virus-recent-advances-in-epidemiology-host-pathogen-interaction-and-vaccine-strategies
#14
REVIEW
Farah Deeba, Asimul Islam, Syed Naqui Kazim, Irshad Hussain Naqvi, Shobha Broor, Anwar Ahmed, Shama Parveen
The Chikungunya virus is a re-emerging alphavirus that belongs to the family Togaviridae. The symptoms include fever, rashes, nausea and joint pain that may last for months. The laboratory diagnosis of the infection is based on the serologic assays, virus isolation and molecular methods. The pathogenesis of the Chikungunya viral infection is not completely understood. Some of the recent investigations have provided information on replication of the virus in various cells and organs. In addition, some recent reports have indicated that the severity of the disease is correlated with the viral load and cytokines...
April 2016: Pathogens and Disease
https://www.readbyqxmd.com/read/26649172/evaluation-of-case-definitions-for-estimation-of-respiratory-syncytial-virus-associated-hospitalizations-among-children-in-a-rural-community-of-northern-india
#15
Siddhartha Saha, Bharti Gaur Pandey, Avinash Choudekar, Anand Krishnan, Susan I Gerber, Sanjay K Rai, Pratibha Singh, Mandeep Chadha, Renu B Lal, Shobha Broor
BACKGROUND: The burden estimation studies for respiratory syncytial virus (RSV) have been based on varied case definitions, including case-definitions designed for influenza surveillance systems. We used all medical admissions among children aged 0-59 months to study the effect of case definitions on estimation of RSV-associated hospitalizations rates. METHODS: The hospital-based daily surveillance enrolled children aged 0-59 months admitted with acute medical conditions from July 2009-December 2012, from a well-defined rural population in Ballabgarh in northern India...
December 2015: Journal of Global Health
https://www.readbyqxmd.com/read/26645038/retrospective-phylogenetic-analysis-of-circulating-ba-genotype-of-human-respiratory-syncytial-virus-with-60%C3%A2-bp-duplication-from-new-delhi-india-during-2007-2010
#16
Vln Raghuram S, Wajihul Hasan Khan, Farah Deeba, Wayne Sullender, Shobha Broor, Shama Parveen
Human respiratory syncytial virus (hRSV) is the most common viral pathogen of acute lower respiratory tract infection in infants and young children. The G protein of hRSV is the trans-membrane glycoprotein that is involved in the attachment of virion with the host cell. The nasopharyngeal aspirates were subjected to RT-PCR for the second hypervariable region of the G protein gene in the present investigation. Sequencing and phylogenetic analysis revealed that all the study strains clustered within the BA genotype...
December 2015: Virusdisease
https://www.readbyqxmd.com/read/26564121/portal-hypertensive-gastropathy-and-gastric-antral-vascular-ectasia
#17
REVIEW
Hitendra Garg, Subhash Gupta, A C Anand, S L Broor
Portal hypertensive gastropathy (PHG) and gastric antral vascular ectasia (GAVE) are gastric mucosal lesions that mostly present as chronic anemia and rarely cause the acute gastrointestinal hemorrhage. Despite similar clinical manifestations, their pathophysiology and management are entirely different. PHG is seen exclusively in patients with portal hypertension, but GAVE can also be observed in patients with other conditions. Their diagnosis is endoscopic, and although generally each of them has a characteristic endoscopic appearance and distribution, there are cases in which the differential is difficult and must rely on histology...
September 2015: Indian Journal of Gastroenterology: Official Journal of the Indian Society of Gastroenterology
https://www.readbyqxmd.com/read/26536458/phylogenetic-and-molecular-clock-analysis-of-dengue-serotype-1-and-3-from-new-delhi-india
#18
Nazia Afreen, Irshad H Naqvi, Shobha Broor, Anwar Ahmed, Shama Parveen
Dengue fever is the most prevalent arboviral disease in the tropical and sub-tropical regions of the world. The present report describes molecular detection and serotyping of dengue viruses in acute phase blood samples collected from New Delhi, India. Phylogenetic and molecular clock analysis of dengue virus serotype 1 and 3 strains were also investigated. Dengue virus infection was detected in 68.87% out of 604 samples tested by RT-PCR between 2011 & 2014. Dengue serotype 1 was detected in 25.48% samples, dengue serotype 2 in 79...
2015: PloS One
https://www.readbyqxmd.com/read/26502931/epidemiology-of-acute-respiratory-infections-in-children-preliminary-results-of-a-cohort-in-a-rural-north-indian-community
#19
Anand Krishnan, Ritvik Amarchand, Vivek Gupta, Kathryn E Lafond, Rizwan Abdulkader Suliankatchi, Siddhartha Saha, Sanjay Rai, Puneet Misra, Debjani Ram Purakayastha, Abhishek Wahi, Vishnubhatla Sreenivas, Arti Kapil, Fatimah Dawood, Chandrakant S Pandav, Shobha Broor, Suresh K Kapoor, Renu Lal, Marc-Alain Widdowson
BACKGROUND: Despite acute respiratory infections being a major cause of death among children in developing countries including India, there is a lack of community-based studies that document its burden and aetiology. METHODS: A dynamic cohort of children aged 0-10 years was established in four villages in a north Indian state of Haryana from August 2012 onwards. Trained health workers conducted weekly home visits to screen children for acute respiratory infection (ARI) defined as one of the following: cough, sore throat, nasal congestion, earache/discharge, or breathing difficulty...
2015: BMC Infectious Diseases
https://www.readbyqxmd.com/read/26444871/biophysical-characterization-of-g-protein-ectodomain-of-group-b-human-respiratory-syncytial-virus-from-e-coli
#20
Wajihul Hasan Khan, V L N Raghuram Srungaram, Asimul Islam, Ilyas Beg, Md Shakir H Haider, Faizan Ahmad, Shobha Broor, Shama Parveen
Human respiratory syncytial virus (hRSV) is an important pathogen of acute respiratory tract infection. The G protein of hRSV is a transmembrane glycoprotein that is a neutralizing antigen and is thus a vaccine candidate. In this study, synthetic codon optimized ectodomain G protein [G(ΔTM)] of BA genotype of group B hRSV was cloned, expressed, and characterized using biophysical techniques. The molar absorption coefficient and mean residue ellipticity at 222 nm ([θ]222) of G (ΔTM) was found to be 7950 M(-1) cm(-1) and -19701...
July 3, 2016: Preparative Biochemistry & Biotechnology
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