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https://www.readbyqxmd.com/read/29156699/cell-specific-regulation-of-proliferation-by-ano1-tmem16a-in-breast-cancer-with-different-er-pr-and-her2-status
#1
Huizhe Wu, Hui Wang, Shu Guan, Jing Zhang, Qiuchen Chen, Xiaodong Wang, Ke Ma, Pengfei Zhao, Haishan Zhao, Weifan Yao, Feng Jin, Qinghuan Xiao, Minjie Wei
The calcium-activated chloride channel Ano1 (TMEM16A) is overexpressed in many tumors. However, conflicting data exist regarding the role of Ano1 in cell proliferation. Here, we performed immunohistochemistry to investigate the expression of Ano1 and Ki67 in 403 patients with breast cancer, and analyzed the association between the expression of Ano1 and Ki67 in breast cancer subtypes categorized according to estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2)...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156385/interaction-with-the-gdf8-11-pathway-reveals-treatment-options-for-adenocarcinoma-of-the-breast
#2
Christoph Wallner, Marius Drysch, Mustafa Becerikli, Henriette Jaurich, Johannes Maximilian Wagner, Stephanie Dittfeld, Julia Nagler, Kamran Harati, Mehran Dadras, Stathis Philippou, Marcus Lehnhardt, Björn Behr
Breast adenocarcinoma continues to be the most frequently diagnosed tumor entity. Despite established therapy options, mortality for breast cancer remains to be as high as 40,000 patients in the US annually. Thus, a need to develop a patient-oriented, targeted therapy exists. In this study, we investigated the interaction of breast adenocarcinoma with the ubiquitously present protein Follistatin and subsequently the GDF8/11 pathway. We analyzed primary histological samples from adenocarcinoma patients for expression of Follistatin and GDF8/11...
November 17, 2017: Breast: Official Journal of the European Society of Mastology
https://www.readbyqxmd.com/read/29154195/drug-loaded-nanoemulsion-as-positive-control-is-an-alternative-to-dmso-solutions-for-in-vitro-evaluation-of-curcumin-delivery-to-mcf-7-cells
#3
Eduarda Schultze, Karine Coradini, Paula Dos Santos Chaves, Liziane Pereira da Silva, Julieti Buss, Silvia S Guterres, Tiago Collares, Ruy Carlos Ruver Beck, Adriana R Pohlmann, Fabiana Kömmling Seixas
BACKGROUND: In vitro evaluation of toxicity and/or efficacy of nanostructured drug delivery systems involves the uses of different controls, including positive and negative controls, as well as a solution or dispersion of the drug in water. One of the most frequently solvent used to dilute poorly water soluble drugs to in vitro tests are dimethylsulfoxide (DMSO). However, its different specific surface area and different diffusion coefficients could make the comparative effects difficult...
May 11, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/29146184/glutamate-production-from-ammonia-via-glutamate-dehydrogenase-2-activity-supports-cancer-cell-proliferation-under-glutamine-depletion
#4
Yukiko Takeuchi, Yasumune Nakayama, Eiichiro Fukusaki, Yasuhiro Irino
Cancer cells rapidly consume glutamine as a carbon and nitrogen source to support proliferation, but the cell number continues to increase exponentially after glutamine is nearly depleted from the medium. In contrast, cell proliferation rates are strongly depressed when cells are cultured in glutamine-free medium. How cancer cells survive in response to nutrient limitation and cellular stress remains poorly understood. In addition, rapid glutamine catabolism yields ammonia, which is a potentially toxic metabolite that is secreted into the extracellular space...
November 13, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29143535/cytotoxic-triterpene-saponins-from-the-mangrove-aegiceras-corniculatum
#5
Le Ba Vinh, Nguyen Thi Minh Nguyet, Seo Young Yang, Jang Hoon Kim, Nguyen Van Thanh, Nguyen Xuan Cuong, Nguyen Hoai Nam, Chau Van Minh, Inkyu Hwang, Young Ho Kim
Using various chromatographic separations, sixteen compounds, including one new triterpene saponin named aegicoroside A (1), were isolated from the leaves of the Vietnamese mangrove Aegiceras corniculatum. Their structures were determined by spectroscopic methods such as 1D and 2D NMR and HR-ESI-MS. The cytotoxic activities of the isolated compounds against MCF7 (breast), HCT116 (colon), B16F10 (melanoma), and A549 (adenocarcinoma) cancer cell lines were also evaluated. Strong cytotoxicity was observed for sakurasosaponin (2) against all four cancer cell lines and for sakurasosaponin methyl ester (3) against MCF7, A549, and HCT116 cell lines with IC50 values ranging from 2...
November 16, 2017: Natural Product Research
https://www.readbyqxmd.com/read/29142397/chemical-composition-antimicrobial-and-antitumor-potentiality-of-essential-oil-of-ferula-tingitana-l-apiaceae-grow-in-libya
#6
Waleed Elghwaji, Abeer Mohamed El-Sayed, Kadriya S El-Deeb, Aly M ElSayed
Background: Ferula tingitana L. (Apiaceae) has been considered to have abortive and menstruation-inducing properties. It used to treat sore throat, fever, indigestion, and pains. Objectives: The objective of this study is to establish the chemical composition of the essential oil of flower, leaves of F. tingitana, and to throw light on antimicrobial, cytotoxic activities of Libyan plant. Materials and Methods: The chemical composition of the essential oil of flower (0...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29141557/halilectin-3-a-lectin-from-the-marine-sponge-haliclona-caerulea-induces-apoptosis-and-autophagy-in-human-breast-cancer-mcf7-cells-through-caspase-9-pathway-and-lc3-ii-protein-expression-haliclona-caerulea-induces-apoptosis-and-autophagy-in-human-breast-cancer
#7
Paula A Videira, Luiz Gonzaga do Nascimento-Neto, Maria Guadalupe Cabral, Romulo Farias Carneiro, Francisco Vassiliepe Sousa Arruda, Zelia Silva, Celso Shiniti Nagano, Alexandre Holanda Sampaio, Edson Holanda Teixeira
BACKGROUND: An ideal strategy for cancer treatment is the specific induction of tumor cell death, sparing normal cells. Marine sponges are rich biological reservoirs of biomolecules, especially lectins, which have attracted considerable attention due to potential biological effect on human cells. Lectins are proteins that bind specific carbohydrate signatures and some gained further interest for their capacity to bind tumor associated carbohydrates antigens and induce tumor cell apoptosis...
November 13, 2017: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/29140506/quantification-of-the-effect-of-toxicants-on-the-intracellular-kinetic-energy-and-cross-sectional-area-of-mammary-epithelial-organoids-by-oct-fluctuation-spectroscopy
#8
Xiao Yu, Ashley M Fuller, Richard Blackmon, Melissa A Troester, Amy L Oldenburg
The ability to assess toxicant exposures of three-dimensional (3D) in vitro mammary models that recapitulate the tissue microenvironment can aid in our understanding of environmental exposure risk over time. Longitudinal studies of 3D model systems, however, are cumbersome and suffer from a lack of high-throughput toxicological assays. In this study, we establish a noninvasive and label-free optical coherence tomography (OCT)-based imaging platform for tracking exposure-response relationships in 3D human mammary epithelial organoid models...
November 10, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29137354/erps294-is-a-biomarker-of-ligand-or-mutational-er%C3%AE-activation-and-a-breast-cancer-target-for-cdk2-inhibition
#9
Gary K Scott, David Chu, Ravneet Kaur, Julia Malato, Daniel E Rothschild, Katya Frazier, Serenella Eppenberger-Castori, Byron Hann, Ben Ho Park, Christopher C Benz
ERα phosphorylation at hinge site S294 (pS294) was recently shown to be essential for ER-dependent gene transcription and mediated by an unknown cyclin-dependent kinase (CDK). This study was undertaken to identify the exact CDK pathway mediating pS294 formation, and to determine if this phosphorylation event occurs with, and can be targeted to treat, the ligand-independent growth of breast cancers expressing endocrine-refractory ESR1 mutations. Using a newly developed anti-pS294 monoclonal antibody, a combination of CDK specific siRNA knockdown studies and a broad panel of CDK selective inhibitors against ligand (E2)-stimulated MCF7 cells, we first identified CDK2 as the primary mediator of pS294 formation and showed that CDK2-selective inhibitors like Dinaciclib, but not CDK4/6 inhibitors like Palbociclib, can selectively prevent pS294 formation and repress ER-dependent gene expression...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29137297/probably-less-than-one-tenth-of-the-genes-produce-only-the-wild-type-protein-without-at-least-one-additional-protein-isoform-in-some-human-cancer-cell-lines
#10
Rui Yan, Ju Zhang, Lucas Zellmer, Lichan Chen, Di Wu, Siqi Liu, Ningzhi Xu, Joshua D Liao
To estimate how many genes produce multiple protein isoforms, we electrophoresed proteins from MCF7 and MDA-MB231 (MB231) human breast cancer cells in SDS-PAGE and excised narrow stripes of the gel at the 48kD, 55kD and 72kD. Proteins in these stripes were identified using liquid chromatography and tandem mass spectrometry. A total of 765, 750 and 679 proteins from MB231 cells, as well as 470, 390 and 490 proteins from MCF7 cells, were identified from the 48kD, 55kD and 72kD stripes, respectively. We arbitrarily allowed a 10% technical variation from the proteins' theoretical molecular mass (TMM) and considered those proteins with their TMMs within the 43-53 kD, 49-61 kD and 65-79 kD ranges as the wild type (WT) expected from the corresponding stripe, whereas those with a TMM above or below this range as a smaller- or larger-group, respectively...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29133279/effect-of-17%C3%AE-estradiol-on-estrogen-receptor-negative-breast-cancer-cells-in-an-osteolytic-mouse-model
#11
D Joshua Cohen, Vaidehi Patel, Anjali Verma, Barbara D Boyan, Zvi Schwartz
17β-Estradiol (E2) promotes metastasis of triple negative breast cancer cells to bone. Recent studies show many triple negative breast cancer cell lines lacking the 66 kDa estrogen receptor (ER) alpha (ERα66) or its splice variant ERα46, express another splice variant, ERα36 associated with membrane-mediated rapid actions of the hormone. qPCR and western blot confirmed that MCF7 cells possessed ERα splice variants ERα66, ERα46 and ERα36, while ER-negative breast cancer cells MDA-MB-231 possessed only ERα36...
November 10, 2017: Steroids
https://www.readbyqxmd.com/read/29130361/mta1-is-a-novel-regulator-of-autophagy-that-induces-tamoxifen-resistance-in-breast-cancer-cells
#12
Min-Ho Lee, Dahae Koh, Hyelin Na, Na-Lee Ka, Seungsu Kim, Hyeon-Ji Kim, Sungyoul Hong, Young Kee Shin, Je Kyung Seong, Mi-Ock Lee
Tamoxifen is commonly used to treat patients with ESR/ER-positive breast cancer, but its therapeutic benefit is limited by the development of resistance. Recently, alterations in macroautophagy/autophagy function were demonstrated to be a potential mechanism for tamoxifen resistance. Although MTA1 (metastasis-associated 1) has been implicated in breast tumorigenesis and metastasis, its role in endocrine resistance has not been studied. Here, we report that the level of MTA1 expression was upregulated in the tamoxifen resistant breast cancer cell lines MCF7/TAMR and T47D/TR, and knockdown of MTA1 sensitized the cells to 4-hydroxytamoxifen (4OHT)...
November 13, 2017: Autophagy
https://www.readbyqxmd.com/read/29128895/raptor-localization-predicts-prognosis-and-tamoxifen-response-in-estrogen-receptor-positive-breast-cancer
#13
Josefine Bostner, Anya Alayev, Adi Y Berman, Tommy Fornander, Bo Nordenskjöld, Marina K Holz, Olle Stål
PURPOSE: Deregulated PI3K/mTOR signals can promote the growth of breast cancer and contribute to endocrine treatment resistance. This report aims to investigate raptor and its intracellular localization to further understand its role in ER-positive breast cancer. METHODS: Raptor protein expression was evaluated by immunohistochemistry in 756 primary breast tumors from postmenopausal patients randomized to tamoxifen or no tamoxifen. In vitro, the MCF7 breast cancer cell line and tamoxifen-resistant MCF7 cells were studied to track the raptor signaling changes upon resistance, and raptor localization in ERα-positive cell lines was compared with that in ERα-negative cell lines...
November 11, 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/29123177/liprotides-kill-cancer-cells-by-disrupting-the-plasma-membrane
#14
Henriette S Frislev, Theresa Louise Boye, Jesper Nylandsted, Daniel Otzen
HAMLET (human α-lactalbumin made lethal to tumour cells) is a complex of α-lactalbumin (aLA) and oleic acid (OA) which kills transformed cells, while leaving fully differentiated cells largely unaffected. Other protein-lipid complexes show similar anti-cancer potential. We call such complexes liprotides. The cellular impact of liprotides, while intensely investigated, remains unresolved. To address this, we report on the cell-killing mechanisms of liprotides prepared by incubating aLA with OA for 1 h at 20 or 80 °C (lip20 and lip80, respectively)...
November 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29119391/comparative-study-of-toxicity-of-alkoxyamines-in-vitro-and-in-vivo
#15
N A Popova, G M Sysoeva, V P Nikolin, V I Kaledin, E V Tretyakov, M V Edeeva, S M Balakhnin, E L Lushnikova, G Audran, S Mark
Effect of alkoxyamines on normal and tumor cells was studied in vitro and in vivo. In vitro experiments showed that alkoxyamines produce a dose-dependent toxic effect on cells of human breast tumor MCF7 line. Transplantation of Krebs-2 ascites carcinoma cells preincubated with alkoxyamines to mice did not induce tumor growth. An opposite effect was observed in normal mouse cells: functional activity of peritoneal macrophages increased. The possibility of using alkoxyamines as theranostic agents is discussed...
November 9, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29116938/mangostanaxanthone-vii-a-new-cytotoxic-xanthone-from-garcinia-mangostana
#16
Sabrin R M Ibrahim, Gamal A Mohamed, Mahmoud A Elfaky, Mohamed F Zayed, Amal A El-Kholy, Osama H Abdelmageed, Samir A Ross
Garcinia mangostana L. (the queen of fruits, mangosteen, family Guttiferae) is a wealthy source of xanthones. The CHCl3 soluble fraction of the air-dried pericarps of G. mangostana provided a new xanthone: mangostanaxanthone VII (5), along with four known xanthones: mangostanaxanthones I (1) and II (2), gartanin (3) and γ-mangostin (4). The structural verification of these metabolites was achieved by different spectral techniques, including UV, IR, 1D and 2D NMR and HRESIMS. The new metabolite was assessed for cytotoxic potential, using sulforhodamine B (SRB) assay towards the A549 and MCF-7 cancer cell lines...
November 8, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/29115987/cancer-cell-soluble-factors-reprogram-mesenchymal-stromal-cells-to-slow-cycling-chemoresistant-cells-with-a-more-stem-like-state
#17
Ahmed El-Badawy, Mohamed A Ghoneim, Mahmoud M Gabr, Radwa Ayman Salah, Ihab K Mohamed, Marwa Amer, Nagwa El-Badri
BACKGROUND: Mesenchymal stem cells (MSCs) play different roles in modulating tumor progression, growth, and metastasis. MSCs are recruited to the tumor site in large numbers and subsequently have an important microenvironmental role in modulating tumor progression and drug sensitivity. However, the effect of the tumor microenvironment on MSC plasticity remains poorly understood. Herein, we report a paracrine effect of cancer cells, in which they secrete soluble factors that promote a more stem-like state in bone marrow mesenchymal stem cells (BM-MSCs)...
November 7, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29115551/p2y2-receptor-promotes-the-migration-and-invasion-of-breast-cancer-cells-via-emt-related-genes-snail-and-e-cadherin
#18
Ying Qiu, Yan Liu, Wei-Hua Li, Hong-Quan Zhang, Xin-Xia Tian, Wei-Gang Fang
Adenosine 5'-triphosphate (ATP) is one of the most abundant biochemical constituents within the tumor microenvironment and is postulated to play critical roles in the progression of a number of types of tumors via interaction with the P2Y2 receptor. In the present study, we demonstrated that the P2Y2 receptor was highly expressed in MCF7 and Hs578T breast cancer cells. Downregulation of the P2Y2 receptor by small interfering RNA (siRNA) significantly attenuated ATP- or UTP-driven migration and invasion of the breast cancer cells as well as expression of EMT-related genes Snail and E-cadherin...
November 7, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29115415/the-stat-ros-cycle-extends-ifn%C3%A2-induced-cancer-cell-apoptosis
#19
Yan Wang, Xiaoyu Yu, Hongtao Song, Di Feng, Yang Jiang, Shuang Wu, Jingshu Geng
In mammals, the signal transducer and activator of transcription (STAT) protein processes mitochondria importation targets and mitochondria respiratory complexes, and triggers reactive oxygen species (ROS) generation, which conversely rapidly initiates the activation of STAT. Interferon (IFN) administration increases cancer cell apoptosis via STAT activation and ROS accumulation. However, the existence of a STAT-ROS cycle and how it affects IFN‑induced cancer cellular apoptosis are unclear. In the present study, we used MCF7 breast cancer cells and confirmed that a combination of IFN‑α/β/γ incubation induced STAT1/3 phosphorylation and mitochondria importation, which increased mitochondria respiratory complexes, the cellular oxygen consumption rate (OCR), and ROS production, followed by cellular apoptosis...
November 8, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/29113888/ultrasensitive-electrochemical-immunosensing-of-tumor-suppressor-protein-p53-in-unprocessed-human-plasma-and-cell-lysates-using-a-novel-nanocomposite-based-on-poly-cysteine-graphene-quantum-dots-gold-nanoparticle
#20
Mohammad Hasanzadeh, Hossein Navay Baghban, Nasrin Shadjou, Ahad Mokhtarzadeh
An ultrasensitive electrochemical immunosensor for quantitation of tumor suppressor protein p53 based on ternary signal amplification strategy was fabricated. In this work, p53-antibody was immobilized onto a green and biocompatible nanocomposite containing poly L-cysteine (P-Cys) as conductive matrix and graphene quantum dots (GQDs)/gold nanoparticles (GNPs) as dual amplification elements. Therefore, a novel multilayer film based on P-Cys, GQDs, and GNPs was exploited to develop a highly sensitive immunosensor for detection of p53...
November 4, 2017: International Journal of Biological Macromolecules
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