Kathryn Geiger-Schuller, Basak Eraslan, Olena Kuksenko, Kushal K Dey, Karthik A Jagadeesh, Pratiksha I Thakore, Ozge Karayel, Andrea R Yung, Anugraha Rajagopalan, Ana M Meireles, Karren Dai Yang, Liat Amir-Zilberstein, Toni Delorey, Devan Phillips, Raktima Raychowdhury, Christine Moussion, Alkes L Price, Nir Hacohen, John G Doench, Caroline Uhler, Orit Rozenblatt-Rosen, Aviv Regev
E3 ligases regulate key processes, but many of their roles remain unknown. Using Perturb-seq, we interrogated the function of 1,130 E3 ligases, partners and substrates in the inflammatory response in primary dendritic cells (DCs). Dozens impacted the balance of DC1, DC2, migratory DC and macrophage states and a gradient of DC maturation. Family members grouped into co-functional modules that were enriched for physical interactions and impacted specific programs through substrate transcription factors. E3s and their adaptors co-regulated the same processes, but partnered with different substrate recognition adaptors to impact distinct aspects of the DC life cycle...
January 24, 2023: bioRxiv