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https://www.readbyqxmd.com/read/28634856/estrogen-receptor-%C3%AE-gene-polymorphism-and-vascular-complications-in-girls-with-type-1-diabetes-mellitus
#1
Bartosz Słomiński, Jolanta Myśliwska, Monika Ryba-Stanisławowska, Maria Skrzypkowska, Małgorzata Myśliwiec
The effect of estrogens is mediated by activation of estrogen receptors (ERs). Because ER-α gene polymorphisms may exert different effects in childhood, we analyzed the associations between the IVS1 -397T>C (PvuII) polymorphism and systemic inflammatory state, proangiogenic factors, frequency of monocyte subsets, lipid profile, blood pressure, and vascular complications in girls with type 1 diabetes mellitus (DM1). We examined 180 young girls with DM1 and 120 healthy age-matched controls. The analysis concerned PvuII polymorphism of the ER-α gene as well as the levels of serum inflammatory markers (CRP, IL-6, TNF-α), proangiogenic factors (VEGF, angiogenin), 17β-estradiol, values of monocyte subsets (CD14(++)CD16(-) and CD14(+)CD16(+)), lipid profile, and blood pressure...
June 20, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28631213/the-constitutive-protease-release-by-primary-human-acute-myeloid-leukemia-cells
#2
Maria Honnemyr, Øystein Bruserud, Annette K Brenner
INTRODUCTION: Acute myeloid leukemia (AML) cells show constitutive release of matrix metalloproteases and their inhibitors. We now investigated this constitutive release of protease/protease regulators associated with carcinogenesis (ADAM12, uPA, cystatin B), angiogenesis (serpin E1, uPA, CD147), cancer cell migration (uPA, cystatin C), coagulation (ADAM TS13, serpin C1), inflammation (fetuin A, caspase 1, cystatin C), monocytic differentiation (CFD) or regulation of hematopoiesis (neutrophil elastase)...
June 19, 2017: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/28630093/dendritic-cell-sensing-of-hydrophobic-di-and-triacylated-lipopeptides-self-assembled-within-synthetic-virus-like-particles
#3
Rajni Sharma, Arin Ghasparian, John A Robinson, Kenneth C McCullough
Dendritic cells (DCs) play critical roles in developing immune defenses. One important aspect is interaction with pathogen-associated molecular patterns (PAMPs)/danger-associated molecular patterns, including di- and triacylated lipopeptides. Isolated or synthetic lipopeptides are potent vaccine adjuvants, interacting with cell surface TLR2 heterodimers. In contrast, deep embedment within bacteria cell walls would impair lipopeptide interaction with cell surface TLR2, requiring degradation for PAMP recognition...
June 19, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28620068/x-box-binding-protein-1-dependent-plasma-cell-responses-limit-the-development-of-atherosclerosis
#4
Andrew P Sage, Meritxell Nus, Jayashree Bagchi Chakraborty, Dimitrios Tsiantoulas, Stephen A Newland, Alison J Finigan, Leanne Masters, Christoph J Binder, Ziad Mallat
Rationale: Diverse B cell responses and functions may be involved in atherosclerosis. Protective antibody responses, such as those against oxidized lipid epitopes, are thought to mainly derive from T cell-independent innate B cell subsets. In contrast, both pathogenic and protective roles have been associated with T cell-dependent antibodies and their importance in both humans and mouse models is still unclear. Objective: To specifically target antibody production by plasma cells and determine the impact on atherosclerotic plaque development in mice with and without CD4+ T cells...
June 15, 2017: Circulation Research
https://www.readbyqxmd.com/read/28619539/systemic-immunological-changes-in-patients-with-distinct-clinical-outcomes-during-mycobacterium-tuberculosis-infection
#5
Tatiane Figueiredo Morais-Papini, Jordana Grazziela Alves Coelho-Dos-Reis, Ana Paula Barbosa Wendling, Lis Ribeiro do Vale Antonelli, Pryscilla Fanini Wowk, Vânia Luiza Deperon Bonato, Valéria Maria Augusto, Silvana Elói-Santos, Olindo Assis Martins-Filho, Cláudia Martins Carneiro, Andréa Teixeira-Carvalho
BACKGROUND: The lung lesions in an individual infected with tuberculosis (TB) are surprisingly variable and independent of each other. However, there is no circulating biomarker yet able to segregate patients according to the extent of lung lesions. MATERIALS AND METHODS: In this study, the phenotypic and functional profile of leukocytes of patients with active pulmonary tuberculosis (TB) and controls (CO) were fully scrutinized by immunophenotyping assays and in vitro short-term whole blood culture...
May 26, 2017: Immunobiology
https://www.readbyqxmd.com/read/28619088/chemokine-signals-are-crucial-for-enhanced-homing-and-differentiation-of-circulating-osteoclast-progenitor-cells
#6
Alan Sucur, Zrinka Jajic, Marinko Artukovic, Marina Ikic Matijasevic, Branimir Anic, Darja Flegar, Antonio Markotic, Tomislav Kelava, Sanja Ivcevic, Natasa Kovacic, Vedran Katavic, Danka Grcevic
BACKGROUND: The peripheral blood (PB) monocyte pool contains osteoclast progenitors (OCPs), which contribute to osteoresorption in inflammatory arthritides and are influenced by the cytokine and chemokine milieu. We aimed to define the importance of chemokine signals for migration and activation of OCPs in rheumatoid arthritis (RA) and psoriatic arthritis (PsA). METHODS: PB and, when applicable, synovial fluid (SF) samples were collected from 129 patients with RA, 53 patients with PsA, and 110 control patients in parallel to clinical parameters of disease activity, autoantibody levels, and applied therapy...
June 15, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28615717/image-based-machine-learning-for-identification-of-macrophage-subsets
#7
Hassan M Rostam, Paul M Reynolds, Morgan R Alexander, Nikolaj Gadegaard, Amir M Ghaemmaghami
Macrophages play a crucial rule in orchestrating immune responses against pathogens and foreign materials. Macrophages have remarkable plasticity in response to environmental cues and are able to acquire a spectrum of activation status, best exemplified by pro-inflammatory (M1) and anti-inflammatory (M2) phenotypes at the two ends of the spectrum. Characterisation of M1 and M2 subsets is usually carried out by quantification of multiple cell surface markers, transcription factors and cytokine profiles. These approaches are time-consuming, require large numbers of cells and are resource intensive...
June 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28615301/irf4-dependent-cd103-cd11b-dendritic-cells-and-the-intestinal-microbiome-regulate-monocyte-and-macrophage-activation-and-intestinal-peristalsis-in-postoperative-ileus
#8
Judith-Mira Pohl, Sebastian Gutweiler, Stephanie Thiebes, Julia K Volke, Ludger Klein-Hitpass, Denise Zwanziger, Matthias Gunzer, Steffen Jung, William W Agace, Daniel Robert Engel, Christian Kurts
OBJECTIVE: Postoperative ileus (POI), the most frequent complication after intestinal surgery, depends on dendritic cells (DCs) and macrophages. Here, we have investigated the mechanism that activates these cells and the contribution of the intestinal microbiota for POI induction. DESIGN: POI was induced by manipulating the intestine of mice, which selectively lack DCs, monocytes or macrophages. The disease severity in the small and large intestine was analysed by determining the distribution of orally applied fluorescein isothiocyanate-dextran and by measuring the excretion time of a retrogradely inserted glass ball...
June 14, 2017: Gut
https://www.readbyqxmd.com/read/28611041/an-immunosuppressive-dendritic-cell-subset-accumulates-at-secondary-sites-and-promotes-metastasis-in-pancreatic-cancer
#9
Justin A Kenkel, William W Tseng, Matthew G Davidson, Lorna Tolentino, Okmi Choi, Nupur Bhattacharya, E Scott Seeley, Daniel Winer, Nathan E Reticker-Flynn, Edgar G Engleman
Pancreatic ductal adenocarcinoma (PDAC) after complete surgical resection is often followed by distant metastatic relapse for reasons that remain unclear. In this study, we investigated how the immune response at secondary sites affects tumor spread in murine models of metastatic PDAC. Early metastases were associated with dense networks of CD11b(+)CD11c(+)MHC-II(+)CD24(+)CD64(low)F4/80(low) dendritic cells (DC), which developed from monocytes in response to tumor-released GM-CSF. These cells uniquely expressed MGL2 and PD-L2 in the metastatic microenvironment and preferentially induced the expansion of T regulatory cells (Treg) in vitro and in vivo...
June 13, 2017: Cancer Research
https://www.readbyqxmd.com/read/28606987/the-fate-and-lifespan-of-human-monocyte-subsets-in-steady-state-and-systemic-inflammation
#10
Amit A Patel, Yan Zhang, James N Fullerton, Lies Boelen, Anthony Rongvaux, Alexander A Maini, Venetia Bigley, Richard A Flavell, Derek W Gilroy, Becca Asquith, Derek Macallan, Simon Yona
In humans, the monocyte pool comprises three subsets (classical, intermediate, and nonclassical) that circulate in dynamic equilibrium. The kinetics underlying their generation, differentiation, and disappearance are critical to understanding both steady-state homeostasis and inflammatory responses. Here, using human in vivo deuterium labeling, we demonstrate that classical monocytes emerge first from marrow, after a postmitotic interval of 1.6 d, and circulate for a day. Subsequent labeling of intermediate and nonclassical monocytes is consistent with a model of sequential transition...
June 12, 2017: Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28606032/metformin-a-new-era-for-an-old-drug-in-the-treatment-of-immune-mediated-disease
#11
Mark Schuiveling, Nadia Vazirpanah, Timothy R D J Radstake, Maili Zimmermann, Jasper C A Broen
Metformin, a widely prescribed blood glucose normalizing antidiabetic drug, is now beginning to receive increasing attention due to its potential anti-inflammatory properties. Metformin suppresses immune responses mainly through its direct effect on the cellular functions of various immune cell types by induction of AMPK and subsequent inhibition of mTORC1, and by inhibition of mitochondrial ROS production. Among key immune events, this results in inhibited monocyte to macrophage differentiation and restrained inflammatory capacity of activated macrophages...
June 12, 2017: Current Drug Targets
https://www.readbyqxmd.com/read/28600970/thp-1-and-human-peripheral-blood-mononuclear-cell-derived-macrophages-differ-in-their-capacity-to-polarize-in-vitro
#12
Hiromi Shiratori, Carmen Feinweber, Sonja Luckhardt, Bona Linke, Eduard Resch, Gerd Geisslinger, Andreas Weigert, Michael J Parnham
Macrophages (Mφ) undergo activation to pro-inflammatory (M1) or anti-inflammatory (M2) phenotypes in response to pathophysiologic stimuli and dysregulation of the M1-M2 balance is often associated with diseases. Therefore, studying mechanisms of macrophage polarization may reveal new drug targets. Human Mφ polarization is generally studied in primary monocyte-derived Mφ (PBMC Mφ) and THP-1-derived Mφ (THP-1 Mφ). We compared the polarization profile of THP-1 Mφ with that of PBMC Mφ to assess the alternative use of THP-1 for polarization studies...
June 7, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28597514/macrophage-migration-inhibitory-factor-induces-inflammation-and-predicts-spinal-progression-in-ankylosing-spondylitis
#13
Vidya Ranganathan, Francesco Ciccia, Fanxing Zeng, Ismail Sari, Guiliana Guggino, Janogini Muralitharan, Eric Gracey, Nigil Haroon
OBJECTIVES: To understand the role of macrophage migration inhibitory factor (MIF) in the pathogenesis of Ankylosing Spondylitis (AS). METHODS: AS patients satisfying the modified New York criteria were recruited for the study. Healthy volunteers, rheumatoid arthritis and osteoarthritis patients were included as controls. Based on the annual rate of increase in mSASSS scores, AS patients were classified as progressors or non-progressors. MIF levels were quantitated by ELISA in the serum and synovial fluid...
June 8, 2017: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/28596372/human-blood-monocyte-subsets-a-new-gating-strategy-defined-using-cell-surface-markers-identified-by-mass-cytometry
#14
Graham D Thomas, Anouk A J Hamers, Catherine Nakao, Paola Marcovecchio, Angela M Taylor, Chantel McSkimming, Anh Tram Nguyen, Coleen A McNamara, Catherine C Hedrick
OBJECTIVE: Human monocyte subsets are defined as classical (CD14(++)CD16(-)), intermediate (CD14(++)CD16(+)), and nonclassical (CD14(+)CD16(+)). Alterations in monocyte subset frequencies are associated with clinical outcomes, including cardiovascular disease, in which circulating intermediate monocytes independently predict cardiovascular events. However, delineating mechanisms of monocyte function is hampered by inconsistent results among studies. APPROACH AND RESULTS: We use cytometry by time-of-flight mass cytometry to profile human monocytes using a panel of 36 cell surface markers...
June 8, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28593077/alteration-in-leukocyte-subsets-and-expressions-of-fc%C3%AE-r-and-complement-receptors-among-female-ragpickers-in-eastern-india
#15
Nandan K Mondal, Shabana Siddique, Madhuchanda Banerjee, Sanghita Roychoudhury, Sayali Mukherjee, Mark S Slaughter, Twisha Lahiri, Manas R Ray
BACKGROUND: There are a million ragpickers in India who gather and trade recyclable municipal solid wastes materials for a living. The objective of this study was to examine whether their occupation adversely affects their immunity. METHODS: Seventy-four women ragpickers (median age, 30 years) and 65 age-matched control housemaids were enrolled. Flow cytometry was used to measure leukocyte subsets, and leukocyte expressions of Fcγ receptor I (CD64), FcγRIII (CD16), complement receptor 1 (CD35) and CR3 (CD11b/CD18), and CD14...
June 2017: Safety and Health At Work
https://www.readbyqxmd.com/read/28591158/mitochondrial-function-of-immune-cells-in-septic-shock-a-prospective-observational-cohort-study
#16
Tobias M Merz, Adriano J Pereira, Roger Schürch, Joerg C Schefold, Stephan M Jakob, Jukka Takala, Siamak Djafarzadeh
BACKGROUND: Reduced cellular ATP synthesis due to impaired mitochondrial function of immune cells may be a factor influencing the immune response in septic shock. We investigate changes in mitochondrial function and bioenergetics of human monocytes and lymphocyte subsets. METHODS: Thirty patients with septic shock were studied at ICU admission, after 24 and 48 hours, and after resolution of shock. Enzymatic activities of citrate synthase and mitochondrial complexes I, IV, and ATP synthase and ATP content of monocytes, T-cells and B-cells and pro-inflammatory (IL-1β and IL-6) and anti-inflammatory (IL-10) cytokine plasma concentrations were compared to samples from 20 healthy volunteers...
2017: PloS One
https://www.readbyqxmd.com/read/28588573/bovine-wc1-and-wc1-neg-%C3%AE-%C3%AE-t-lymphocytes-influence-monocyte-differentiation-and-monocyte-derived-dendritic-cell-maturation-during-in-vitro-mycobacterium-avium-subspecies-paratuberculosis-infection
#17
Monica M Baquero, Brandon L Plattner
During early Mycobacterium avium subspecies paratuberculosis (Map) infection, complex interactions occur between the bacteria, cells from the mononuclear phagocyte system (MPS) including both resident (macrophages and dendritic cells) and recruited (monocytes) cells, and other mucosal sentinel cells such as γδ T lymphocytes. Though the details of early host-pathogen interactions in cattle remain largely underexplored, our hypothesis is that these significantly influence development of host immunity and ultimate success or failure of the host to respond to Map infection...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28586426/sensitive-detection-of-zika-virus-antigen-in-patients-whole-blood-as-an-alternative-diagnostic-approach
#18
Fok-Moon Lum, Cui Lin, Olga Y Susova, Teck-Hui Teo, Siew-Wai Fong, Tze-Minn Mak, Linda Kay Lee, Chia-Yin Chong, David C B Lye, Raymond T P Lin, Andres Merits, Yee-Sin Leo, Lisa F P Ng
Background.: Epidemics caused by the re-emergence of Zika virus (ZIKAV) warrant the need to develop new diagnostic measures to complement currently used detection methods. In this study, we explored the detection of ZIKAV antigen in a defined leucocyte subset from patient's whole blood. Methods.: Whole blood samples were obtained at the acute and early convalescent phase from ZIKAV-infected patients during the Singapore outbreak in Aug-Sep 2016. Presence of ZIKAV antigen was determined by flow cytometry staining for intracellular ZIKAV NS3 with a ZIKAV-specific polyclonal antibody...
June 6, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28585414/tissue-factor-variants-induce-monocyte-transformation-and-transdifferentiation-into-endothelial-cell-like-cells
#19
G Arderiu, S Espinosa, E Peña, J Crespo, R Aledo, V Y Bogdanov, L Badimon
BACKGROUND: Monocytes (Mo) increase neovascularization by releasing proangiogenic mediators and/or transdifferentiating into endothelial cell-like (ECL). Recently, we have reported that Mo-microvascular endothelial cells (mECs) crosstalk induces mEC-tissue factor (TF) expression and promotes angiogenesis. However, the effect of TF on Mo remains unknown. OBJECTIVE: Here, we analyzed whether TF might exert angiogenic effects by inducing transdifferentiation of Mo...
June 6, 2017: Journal of Thrombosis and Haemostasis: JTH
https://www.readbyqxmd.com/read/28578937/active-hcv-infection-is-associated-with-increased-circulating-levels-of-interferon-gamma-ifn-%C3%AE-inducible-protein-10-ip-10-soluble-cd163-and-inflammatory-monocytes-regardless-of-liver-fibrosis-and-hiv-coinfection
#20
Claudia Mascia, Miriam Lichtner, Paola Zuccalà, Serena Vita, Tiziana Tieghi, Raffaella Marocco, Stefano Savinelli, Raffaella Rossi, Marco Iannetta, Michela Campagna, Francesco Schiavone, Fabio Mengoni, Gianluca Russo, Claudio Maria Mastroianni, Vincenzo Vullo
BACKGROUND AND OBJECTIVE: Interferon-gamma (IFN-γ)-inducible protein-10 (IP-10), soluble (s) CD163 and sCD14 play an important role in the pathogenesis of HCV and HIV infection and are involved in inflammation and liver fibrosis. The aim of the present study was to evaluate at a single time point, plasma soluble biomarkers and inflammatory monocytes subsets in different groups of subjects: (i) HIV monoinfected patients on suppressive ART; (ii) HIV/HCV coinfected patients on ART, with undetectable HIV viremia (including either subjects who had active HCV replication or those who cleared HCV); (iii) HCV monoinfected individual with active viral replication...
May 31, 2017: Clinics and Research in Hepatology and Gastroenterology
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