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https://www.readbyqxmd.com/read/29235972/decoding-dna-labels-by-melting-curve-analysis-using-real-time-pcr
#1
József A Balog, Liliána Z Fehér, László G Puskás
Synthetic DNA has been used as an authentication code for a diverse number of applications. However, existing decoding approaches are based on either DNA sequencing or the determination of DNA length variations. Here, we present a simple alternative protocol for labeling different objects using a small number of short DNA sequences that differ in their melting points. Code amplification and decoding can be done in two steps using quantitative PCR (qPCR). To obtain a DNA barcode with high complexity, we defined 8 template groups, each having 4 different DNA templates, yielding 158 (>2...
December 1, 2017: BioTechniques
https://www.readbyqxmd.com/read/29234181/urban-rural-differences-in-the-prevalence-of-self-reported-diabetes-and-its-risk-factors-the-who-steps-iranian-noncommunicable-disease-risk-factor-surveillance-in-2011
#2
Zahra Khorrami, Shahin Yarahmadi, Koorosh Etemad, Soheila Khodakarim, Mohammad Esmaeil Kameli, Ali Reza Mahdavi Hazaveh
The high prevalence of diabetes in Iran and other developing countries is chiefly attributed to urbanization. The objectives of the present study were to assess the prevalence of self-reported diabetes and to determine its associated risk factors. This study is a part of the national noncommunicable disease risk factor surveillance, conducted in 31 provinces of Iran in 2011. First, 10069 individuals, between 20 and 70 years old (3036 individuals from rural and 7033 from urban areas), were recruited. The major risk factors were studied using a modified WHO STEPS approach...
September 2017: Iranian Journal of Medical Sciences
https://www.readbyqxmd.com/read/29234051/lagrangian-statistics-and-intermittency-in-gulf-of-mexico
#3
Liru Lin, Wei Zhuang, Yongxiang Huang
Due to the nonlinear interaction between different flow patterns, for instance, ocean current, meso-scale eddies, waves, etc, the movement of ocean is extremely complex, where a multiscale statistics is then relevant. In this work, a high time-resolution velocity with a time step 15 minutes obtained by the Lagrangian drifter deployed in the Gulf of Mexico (GoM) from July 2012 to October 2012 is considered. The measured Lagrangian velocity correlation function shows a strong daily cycle due to the diurnal tidal cycle...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29232861/detection-of-network-motif-based-on-a-novel-graph-canonization-algorithm-from-transcriptional-regulation-networks
#4
Jialu Hu, Xuequn Shang
Network motifs are patterns of complex networks occurring significantly more frequently than those in random networks. They have been considered as fundamental building blocks of complex networks. Therefore, the detection of network motifs in transcriptional regulation networks is a crucial step in understanding the mechanism of transcriptional regulation and network evolution. The search for network motifs is similar to solving subgraph searching problems, which has proven to be NP-complete. To quickly and effectively count subgraphs of a large biological network, we propose a novel graph canonization algorithm based on resolving sets...
December 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29232748/-combined-posteromedial-and-anterolateral-approach-in-the-floating-position-two-column-fracture-of-the-tibia-head
#5
Maike Reul, Harm Hoekstra
Background The posterior tibial plateau is often affected in intra-articular tibial fractures. Moreover, treating these posterior fractures is recognised as an important prognostic factor. Open reduction and internal fixation of lateral and posterior two column tibial plateau fractures can be achieved via a combined reversed L-shape approach and an anterolateral approach in the floating position without intraoperative repositioning of the patient. Material and Methods The operative procedure starts in the posterior position with visualisation of the posterior column after lateral retraction of the medial head of the M...
December 2017: Zeitschrift Für Orthopädie und Unfallchirurgie
https://www.readbyqxmd.com/read/29230815/topographies-of-care-pathways-and-healthscapes-reconsidering-the-multiple-journeys-of-people-with-a-brain-tumour
#6
Henry Llewellyn, Paul Higgs, Elizabeth L Sampson, Louise Jones, Lewis Thorne
People diagnosed with brain tumours enter new and unfamiliar worlds in which they must make complex and previously unimaginable decisions about care, treatment and how to live their lives. While decisions are increasingly based around care pathways, these are embedded in values that often fail to accord with those of patients. In this article, we examine the cases of people with a brain tumour and how they, their families and healthcare professionals navigate and intervene in the course of life-threatening disease...
December 12, 2017: Sociology of Health & Illness
https://www.readbyqxmd.com/read/29229983/annotation-free-quantification-of-rna-splicing-using-leafcutter
#7
Yang I Li, David A Knowles, Jack Humphrey, Alvaro N Barbeira, Scott P Dickinson, Hae Kyung Im, Jonathan K Pritchard
The excision of introns from pre-mRNA is an essential step in mRNA processing. We developed LeafCutter to study sample and population variation in intron splicing. LeafCutter identifies variable splicing events from short-read RNA-seq data and finds events of high complexity. Our approach obviates the need for transcript annotations and circumvents the challenges in estimating relative isoform or exon usage in complex splicing events. LeafCutter can be used both to detect differential splicing between sample groups and to map splicing quantitative trait loci (sQTLs)...
December 11, 2017: Nature Genetics
https://www.readbyqxmd.com/read/29228770/polyelectrolyte-capping-as-straightforward-approach-towards-manipulation-of-diffusive-transport-in-mof-films
#8
Juan Alejandro Allegretto, Jimena S Tuninetti, Agustin Lorenzo, Marcelo Ceolin, Omar Azzaroni, Matias Rafti
We present experimental results demonstrating the suitability of polyelectrolyte capping as a simple and straightforward procedure to modify hydrophilic/hydrophobic character of porous films, thus allowing additional control on transport properties. In particular, we synthesized ZIF-8 Metal Organic Framework (MOF) films, an archetypal hydrophobic Zeolitic Imidazolate Framework, constituted by Zn2+ ions tetrahedrally coordinated with bidentate 2-methylimidazolate organic linker, and Poly(4-styrenesulfonic acid) as capping agent (PSS)...
December 11, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29227721/chinese-guidelines-for-childhood-asthma-2016-major-updates-recommendations-and-key-regional-data
#9
Jianguo Hong, Yixiao Bao, Aihuan Chen, Changchong Li, Li Xiang, Chuanhe Liu, Zhimin Chen, Deyu Zhao, Zhou Fu, Yunxiao Shang
OBJECTIVE: With increased industrialization and urbanization in China, pediatric asthma is becoming more prevalent. Despite a growing body of evidence, there remains a significant unmet need for adequate management of childhood asthma. The Subspecialty Group of Respiratory Diseases of the Society of Pediatrics, the Chinese Medical Association, and the editorial board of the Chinese Journal of Pediatrics have recently updated the "Guidelines for diagnosis and optimal management of asthma in children," first published in 2008...
December 11, 2017: Journal of Asthma: Official Journal of the Association for the Care of Asthma
https://www.readbyqxmd.com/read/29226487/prenatal-diagnostic-testing-and-atypical-chromosome-abnormalities-following-combined-first-trimester-screening-implications-for-contingent-models-of-non-invasive-prenatal-testing
#10
Anthea Lindquist, Alice Poulton, Jane Halliday, Lisa Hui
OBJECTIVES: To perform a population-based analysis of a combined first trimester screening (CFTS) cohort for (i) changes in uptake of invasive prenatal diagnosis by CFTS risk, and (ii) prevalence and methods of ascertainment of atypical chromosome abnormalities. METHODS: Retrospective cohort study of state-wide prenatal datasets from Victoria, Australia. A three-step approach was undertaken: i) record-linkage between serum screening and diagnostic results; ii) comparison of rates of diagnostic testing by CFTS risk categories in a 2014-15 CFTS cohort with a historical cohort from 2002-04; (iii) detailed analysis of atypical abnormalities from 2014-15 by CFTS risk, individual serum analyte level and indications for diagnostic testing...
December 11, 2017: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29225906/patient-engagement-in-kidney-research-opportunities-and-challenges-ahead
#11
REVIEW
Amber O Molnar, Moumita Barua, Ana Konvalinka, Kara Schick-Makaroff
Purpose of Review: Patient engagement in research is increasingly recognized as an important component of the research process and may facilitate translation of research findings. To heighten awareness on this important topic, this review presents opportunities and challenges of patient engagement in research, drawing on specific examples from 4 areas of Canadian kidney research conducted by New Investigators in the Kidney Research Scientist Core Education and National Training (KRESCENT) Program...
2017: Canadian Journal of Kidney Health and Disease
https://www.readbyqxmd.com/read/29224628/co-immobilization-of-enoate-reductase-with-a-cofactor-recycling-partner-enzyme
#12
Han Li, Wenhua Xiao, Panpan Xie, Liangyu Zheng
Herein we established co-immobilized methods for enoate reductases (ERs) and glucose dehydrogenase (GDH), forming a cofactor regeneration system. In cross-linked enzyme aggregates (CLEAs), ammonium sulfate and oxidized dextran were selected as a precipitant and a cross-linker, respectively. In biomimetic immobilization (BI), ER-GDH-silica particles (ER-GDH-SPs) were rapidly formed through a one-step approach by using a silicic acid precursor. Under the optimal conditions, the ER activity recovery in ER-GDH-CLEAs and ER-GDH-SPs were 44...
February 2018: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/29223715/development-of-two-fluorine-18-labeled-pet-radioligands-targeting-pde10a-and-in-vivo-pet-evaluation-in-nonhuman-primates
#13
Vladimir Stepanov, Akihiro Takano, Ryuji Nakao, Nahid Amini, Shotaro Miura, Tomoaki Hasui, Haruhide Kimura, Takahiko Taniguchi, Christer Halldin
INTRODUCTION: Phosphodiesterase 10A (PDE10A) is a member of the PDE enzyme family that degrades cyclic adenosine and guanosine monophosphates (cAMP and cGMP). Based on the successful development of [11C]T-773 as PDE10A positron emission tomography (PET) radioligand, in this study our aim was to develop and evaluate fluorine-18 analogs of [11C]T-773. METHODS: [18F]FM-T-773-d2 and [18F]FE-T-773-d4 were synthesized from the same precursor used for 11C-labeling of T-773 in a two-step approach via 18F-fluoromethylation and 18F-fluoroethylation, respectively, using corresponding deuterated synthons...
October 23, 2017: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/29221743/ex%C3%A2-vivo-hydrodynamics-after-central-and-paracommissural-edge-to-edge-technique-a-further-step-toward-transcatheter-tricuspid-repair
#14
Sina Stock, Heidemarie Bohm, Michael Scharfschwerdt, Doreen Richardt, Roza Meyer-Saraei, Stanislav Tsvelodub, Hans-Hinrich Sievers
OBJECTIVES: Transcatheter approaches in heart valve disease became tremendously important and are currently established in the aortic position, but transcatheter tricuspid repair is still in its beginning and remains challenging. Replicating the surgical edge-to-edge technique, for example, with the MitraClip System (Abbott Vascular, Santa Clara, Calif), represents a promising option and has been reported successfully in small numbers of cases. However, up to now, few data considering the edge-to-edge technique as a transcatheter approach are available...
November 2, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/29221434/longitudinal-drop-out-and-weighting-against-its-bias
#15
Steffen C E Schmidt, Alexander Woll
BACKGROUND: The bias caused by drop-out is an important factor in large population-based epidemiological studies. Many studies account for it by weighting their longitudinal data, but to date there is no detailed final approach for how to conduct these weights. METHODS: In this study we describe the observed longitudinal bias and a three-step longitudinal weighting approach used for the longitudinal data in the MoMo baseline (N = 4528, 4-17 years) and wave 1 study with 2807 (62%) participants between 2003 and 2012...
December 8, 2017: BMC Medical Research Methodology
https://www.readbyqxmd.com/read/29221315/four-arm-robotic-assisted-pulmonary-resection-right-upper-lobectomy-how-to-do-it
#16
Alessandro Pardolesi, Luca Bertolaccini, Jury Brandolini, Piergiorgio Solli
Numerous published articles have shown the safety and efficacy of robotic lung resection, including lobectomy. Several techniques have been described by different authors to perform a robot assisted lung lobectomy. We adopted four arms robotic procedure. We usually perform three ports (10-15 mm) and an anterior 3 to 4 cm utility incision. This technique allows to safely proceed with an anterior to posterior approach to the hilum. In this study, we present in clear and sequential steps, the procedure to perform a right upper lobectomy for early stage lung cancer...
September 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29220425/a-leaflet-plication-clip-is-an-effective-surgical-template-for-mitral-valve-foldoplasty
#17
Isaac Wamala, Mossab Saeed, Sunil J Ghelani, Kimberlee Gauvreau, Peter E Hammer, Nikolay V Vasilyev, Pedro J Del Nido
OBJECTIVES: During mitral valve repair using the foldoplasty technique, correct judgement of the necessary dimensions and orientation of the leaflet fold is a critical but challenging step that can affect the chances of successful repair. In this study, we investigated whether a leaflet plication clip can be used to guide suture foldoplasty for posterior leaflet prolapse of the mitral valve. METHODS: Complete posterior leaflet prolapse was created in both in vivo and ex vivo pig hearts by severing the chordae supporting the middle scallop...
December 6, 2017: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/29216780/using-cost-analyses-to-inform-health-professions-education-the-economic-cost-of-pre-clinical-failure
#18
Jonathan Foo, Dragan Ilic, George Rivers, Darrell J R Evans, Kieran Walsh, Terry P Haines, Sophie Paynter, Prue Morgan, Stephen Maloney
BACKGROUND: Student failure creates additional economic costs. Knowing the cost of failure helps to frame its economic burden relative to other educational issues, providing an evidence-base to guide priority setting and allocation of resources. The Ingredients Method is a cost-analysis approach which has been previously applied to health professions education research. In this study, the Ingredients Method is introduced, and applied to a case study, investigating the cost of pre-clinical student failure...
December 7, 2017: Medical Teacher
https://www.readbyqxmd.com/read/29215875/a-nanopore-lithography-strategy-towards-hierarchically-micro-mesoporous-carbon-from-zif-8-graphene-oxide-hybrids-for-electrochemical-energy-storage
#19
Francisco Julian Martin-Jimeno, Fabian Suárez-García, Juan Ignacio Paredes, Marina Enterria, Manuel Fernando R Pereira, José Inácio Martins, José L Figueiredo, Amelia Martinez-Alonso, Juan Manuel D Tascon
Porous carbons derived from metal-organic frameworks (MOFs) are promising materials towards a number of energy- and environment-related applications, but their almost exclusively microporous texture can be an obstacle to their performance in practical uses. Here, we introduce a novel strategy for the generation of very uniform mesoporosity in a prototypical MOF, namely the zeolitic imidazolate framework-8 (ZIF-8). The process, which is referred to as "nanopore lithography", makes use of graphene oxide (GO) nanosheets enclosing ZIF-8 particles as masks or templates for the transfer of mesoporous texture to the latter...
December 7, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29212377/gene-therapy-for-bone-repair-using-human-cells-superior-osteogenic-potential-of-bmp-2-transduced-mesenchymal-stem-cells-derived-from-adipose-tissue-compared-to-bone-marrow
#20
Sofia Bougioukli, Osamu Sugiyama, William Pannell, Brandon Ortega, Matthew Tan, Amy Tang, Robert Yoho, Daniel A Oakes, Jay R Lieberman
Ex vivo regional gene therapy strategies using animal mesenchymal stem cells genetically modified to overexpress osteoinductive growth factors has been successfully used in a variety of animal models to induce both heterotopic and orthotopic bone formation. However, in order to adapt regional gene therapy for clinical applications it is essential to assess the osteogenic capacity of transduced human cells and choose the cell type that demonstrates the best clinical potential. Bone marrow stem cells (BMSC) and adipose-derived stem cells (ASC) were selected in our study for in vitro evaluation, before and after transduction with a lentiviral two-step transcriptional amplification system (TSTA) overexpressing BMP-2 (LV-TSTA-BMP-2) or GFP (LV-TSTA-GFP); cell growth, transduction efficiency, BMP-2 production and osteogenic capacity were assessed...
December 6, 2017: Human Gene Therapy
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