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Joakim Bjerketorp, Wilfred F M Röling, Xin-Mei Feng, Armando Hernández Garcia, Hermann J Heipieper, Sebastian Håkansson
Chlorophenols are widespread and of environmental concern due to their toxic and carcinogenic properties. Development of less costly and less technically challenging remediation methods are needed; therefore, we developed a formulation based on micronized vermiculite that, when air-dried, resulted in a granular product containing the 4-chlorophenol (4-CP)-degrading Gram-positive bacterium Arthrobacter chlorophenolicus A6. This formulation and stabilization method yielded survival rates of about 60% that remained stable in storage for at least 3 months at 4 °C...
January 18, 2018: Applied Microbiology and Biotechnology
S G Badman, L M Causer, R Guy, H Wand, B Donovan, S N Tabrizi, D Speers, M D Shephard, A Vallely, D Whiley
Quality control (QC) is an essential component of point-of-care testing programs. In the context of a randomised-controlled trial (TTANGO) using GeneXpert (Xpert) Chlamydia trachomatis and Neisseria gonorrhoeae (CT/NG) point-of-care testing in remote areas of Australia, we aimed to develop and utilise a stable positive control material. Bacterial cultures of CT and NG were resuspended together to provide cycle threshold (Ct) values of approximately 25 cycles for both CT and NG when tested on the Xpert CT/NG assay...
January 11, 2018: Pathology
Yamjala Ganeshkumar, Ajmera Ramarao, Ciddi Veeresham
Background: Picrorhiza kurroa (PK) belongs to Scrophulariaceae family and is a representative endemic, medicinal herb, widely distributed throughout the higher altitudes of alpine Himalayas from west to east, between 3000 and 4500 m above mean sea level. Objective: The objective of the present study is to assess the production of picroside I and picroside II from tissue cultures of PK. Materials and Methods: Auxiliary shoot tips of PK were incubated in Murashige and Skoog medium supplemented with indole-3-butyric acid and kinetin phytohormones...
December 2017: Pharmacognosy Research
Yumi Fujiwara, Miya Kono, Airi Ito, Michiho Ito
High-quality perilla leaves are purple on upper and lower surfaces and have a good aroma. The Japanese Pharmacopoeia specifies the content of essential oils in perilla leaves but not that of anthocyanins. Several reports have described the chemical species of anthocyanins in red perilla, but a complete analysis of anthocyanins in perilla has not been reported. In this study, the anthocyanins in the leaves of cultivated and wild species of perilla and those in commercially available perilla herbs were studied...
January 11, 2018: Phytochemistry
Gabriel M Mascarin, Nilce N Kobori, Mark A Jackson, Christopher A Dunlap, Ítalo Delalibera
AIMS: Nitrogen is a critical element in industrial fermentation media. This study investigated the influence of various nitrogen sources on blastospore production, desiccation tolerance, and storage stability using two strains of the cosmopolitan insect pathogenic fungus Beauveria bassiana. METHODS AND RESULTS: Complex organic sources of nitrogen such as soy flour, autolyzed yeast and cottonseed flour induced great numbers of blastospores after 2-3 days of fermentation, which also survived drying and remained viable (32-56% survival) after 9 months storage at 4 °C, although variations were found between strains...
January 11, 2018: Journal of Applied Microbiology
Navina Ramakrishnan, Swati Sharma, Arun Gupta, Basma Yahya Alashwal
Plastics have been one of the highly valued materials and it plays an significant role in human's life such as in food packaging and biomedical applications. Bioplastic materials can gradually work as a substitute for various materials based on fossil oil. The issue like sustainability and environmental challenges which occur due to manufacturing and disposal of synthetic plastics can be conquering by bio-based plastics. Feathers are among the most inexpensive abundant, and renewable protein sources. Feathers disposal to the landfills leads to environmental pollutions and it results into wastage of 90% of protein raw material...
January 7, 2018: International Journal of Biological Macromolecules
Kai Fan, Min Zhang, Arun S Mujumdar
Microwave heating has been applied in the drying of high-value solids as it affords a number of advantages, including shorter drying time and better product quality. Freeze-drying at cryogenic temperature and extremely low pressure provides the advantage of high product quality, but at very high capital and operating costs due partly to very long drying time. Freeze-drying coupled with a microwave heat source speeds up the drying rate and yields good quality products provided the operating unit is designed and operated to achieve the potential for an absence of hot spot developments...
January 10, 2018: Critical Reviews in Food Science and Nutrition
Saleem Ullah, Ismail Zainol, Shiplu Roy Chowdhury, M B Fauzi
The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility. The scaffolds were fabricated via freeze-drying technique. The effects of various compositions consisting in 3D scaffolds were investigated via FT-IR analysis, porosity, swelling and mechanical tests, and effect on the morphology of scaffolds investigated microscopically...
January 2, 2018: International Journal of Biological Macromolecules
I Issoufou, M Lakranbi, L Belliraj, F Z Ammor, H Harmouchi, Y Ouadnouni, M Smahi
INTRODUCTION: Tuberculous pyothorax or empyema is one of the serious forms of tuberculosis and still poses public health problems. Through a series of patients who undergone pleuropulmonary decortication, we propose our model of management and determine the main factors prognostic. METHOD: We retrospectively retrieved for 8 years 93 cases of patients with pleuropulmonary decortication for tuberculous pyothorax confirmed by histological examination pre- or postoperatively...
December 28, 2017: Revue de Pneumologie Clinique
Hani Nassar, Hassan El-Shamy
Aim and Objective: This survey investigated bonding system type and composite application rationale by senior dental students at a dental school in Saudi Arabia. Materials and Methods: Eighty-three questionnaires with 15 open and closed questions were randomly distributed to final year dental students about the use of etch and rinse versus self-etch adhesives and application techniques for posterior resin composite. Data were collected, and statistical analysis was done using Chi-square test (0...
November 2017: Journal of International Society of Preventive & Community Dentistry
Sara Capiau, Leah S Wilk, Pieter M M de Kesel, Maurice C G Aalders, Christophe P Stove
The hematocrit (Hct) effect is one of the most important hurdles currently preventing more widespread implementation of quanti-tative dried blood spot (DBS) analysis in a routine context. Indeed, the Hct may affect both the accuracy of DBS methods, as well as the interpretation of DBS-based results. We previously developed a method to determine the Hct of a DBS based on its hemoglobin content using non-contact diffuse reflectance spectroscopy. Despite the ease with which the analysis can be performed (i.e. mere scanning of the DBS) and the good results which were obtained, the method did require a complicated algorithm to derive the total hemoglobin content from the DBS's reflectance spectrum...
December 27, 2017: Analytical Chemistry
T G Schulman, R S Samlal-Soedhoe, J van der Weerd
The accuracy of textile damage analyses was evaluated by laboratory tests carried out by trained experts. The analyzed damages were prepared by various methods, including stabbing, cutting, shooting, heating/burning, etc. A number of damages were aged by household washing and tumble-drying procedures, addition of blood, or burying. The samples were analyzed by routine laboratory evaluation. The results indicate that the properties of a damage provide a good indication of the way a textile had been damaged. Nevertheless, scoring of the answers is not straightforward...
January 2018: Forensic Science Review
Kazuko Omodaka, Guangzhou An, Satoru Tsuda, Yukihiro Shiga, Naoko Takada, Tsutomu Kikawa, Hidetoshi Takahashi, Hideo Yokota, Masahiro Akiba, Toru Nakazawa
PURPOSE: This study aimed to develop a machine learning-based algorithm for objective classification of the optic disc in patients with open-angle glaucoma (OAG), using quantitative parameters obtained from ophthalmic examination instruments. METHODS: This study enrolled 163 eyes of 105 OAG patients (age: 62.3 ± 12.6, mean deviation of Humphrey field analyzer: -8.9 ± 7.5 dB). The eyes were classified into Nicolela's 4 optic disc types by 3 glaucoma specialists...
2017: PloS One
Min Jiang, Houzhi Li, Lijuan Zhou, Ruofei Xing, Jianming Zhang
Hierarchical zeolitic imidazole framework (ZIFs) combining micropore with mesoporous structure is desirable to enhance mass transport and give rise to novel applications. Here, hierarchically porous graphene/ZIF-8 hybrid aerogel (GZAn) materials were successfully prepared by a two-step reduction strategy and layer-by-layer assembly method. To avoid a tedious dry step and a use of energy-consuming freeze-drying technology, a reduced graphene oxide hydrogel with different reduction degree was chosen as template to in-situ grow ZIF-8 crystals...
December 20, 2017: ACS Applied Materials & Interfaces
Pieter-Jan Van Bockstal, Séverine Thérèse F C Mortier, Jos Corver, Ingmar Nopens, Krist V Gernaey, Thomas De Beer
Pharmaceutical batch freeze-drying is commonly used to improve the stability of biological therapeutics. The primary drying step is regulated by the dynamic settings of the adaptable process variables, shelf temperature Ts and chamber pressure Pc. Mechanistic modelling of the primary drying step leads to the optimal dynamic combination of these adaptable process variables in function of time. According to Good Modelling Practices, a Global Sensitivity Analysis (GSA) is essential for appropriate model building...
December 16, 2017: European Journal of Pharmaceutics and Biopharmaceutics
Yu-Wen Ting, Wei-Tang Chang, Duen-Kai Shiau, Pei-Hsuan Chou, Mei-Fang Wu, Chin-Lin Hsu
The anti-obesity effects of quercetin-rich supplement (QRS), which contain quercetin, lycopene, taurine, and litchi flower extract, on a high-fat diet (HFD)-induced obese rats were investigated. The rats that consume HFD with QRS (185 mg/kg rat) have significantly modulated the final body weights [490±11 (HFD)→441±11 (HFD+QRS) g], total body fat [112.9±4.5 (HFD)→86.6±5.7 (HFD+QRS) g], liver weights [14.8±0.4 (HFD)→12.6±0.4 (HFD+QRS) g/rat], and the serum TG [102.5±7.3 (HFD)→90.7±6.5 (HFD+QRS) mg/dL] to a level that resembled the regular diet-consumed rats (p<0...
December 18, 2017: Journal of Agricultural and Food Chemistry
N Brogna, A Palmonari, G Canestrari, L Mammi, A Dal Prà, A Formigoni
In vitro and in situ procedures performed to estimate indigestible neutral detergent fiber (iNDF) in forage or fecal samples are time consuming, costly, and limited by intrinsic factors. In contrast, near infrared reflectance spectroscopy (NIRS) has become widely recognized as a valuable tool for accurately determining chemical composition and digestibility parameters of forages. The aim of this study was to build NIRS calibrations and equations for fecal iNDF. In total, 1,281 fecal samples were collected to build a calibration data set, but only 301 were used to develop equations...
December 13, 2017: Journal of Dairy Science
Jesper Håkansson, Bart Kuyken, Dries Van Thourhout
As resonance mass sensors shrink in order to improve the sensitivity, traditional methods of transduction and readout struggle to keep up with increasing resonance frequencies and decreasing feature sizes. In this work we demonstrate an all-photonically transduced resonant mass sensor that manages to deal with these problems. The strong optomechanical force in slot waveguides is used to drive the mechanical resonanator giving a good signal to noise ratio at low optical powers. Using 120 µW of modulated optical power we measure a frequency noise equivalent to being able to resolve 500 kDa...
December 11, 2017: Optics Express
Štěpán Strnad, Vladimír Vrkoslav, Zuzana Klimšová, Jana Zemenová, Josef Cvačka, Lenka Maletínská, David Sýkora
AIM: To demonstrate and discuss the pros and cons of various conventional and innovative analytical approaches. Methodology & results: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MSI) of tissue sections as well as the extraction of tissue homogenates, blood plasma and dried blood spots coupled with LC-MS were employed to monitor the pharmacokinetics of metformin in mice. The time profile of metformin measured by matrix-assisted laser desorption/ionization MSI correlated well with the results found by LC-MS...
December 15, 2017: Bioanalysis
Qingge Feng, Huidong Cai, Haiying Lin, Siying Qin, Zheng Liu, Dachao Ma, Yuyang Ye
TiO2 aerogel is a unique three-dimensional porous nano-particle material with the characteristics of high specific surface area and good light transmittance. In this paper, a novel method involving ultrasonic-assisted sol-gel, solvent exchange, and vacuum drying was successfully developed to synthesis the TiO2 aerogel. The morphology and properties of the prepared TiO2 aerogels were characterized by Brunauer-Emmett-Teller theory (BET), X-ray diffraction (XRD), Field-emission scanning electron microscopy (FESEM), Energy dispersive spectroscopy (EDS), Thermogravimetric analysis-Differential thermal analysis (TGA-DTA), Fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy...
December 14, 2017: Nanotechnology
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