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https://www.readbyqxmd.com/read/28721410/an-intrinsic-internal-standard-substrate-of-au-ps-b-p4vp-for-rapid-quantification-by-surface-enhanced-raman-scattering
#1
Jiamin Gao, Cheng Zhao, Zhuomin Zhang, Gongke Li
Reliable quantification by surface enhanced Raman scattering (SERS) highly depends on the development of a reproducible substrate with excellent anti-interference capability. In this work, an intrinsic internal standard (IS) SERS substrate based on a diblock copolymer, polystyrene-block-poly-4-vinylpyridine (PS-b-P4VP), as a template was developed by a modified block copolymer lithography (BCL) technique of reserving the diblock copolymer template. This substrate generated a stable vibration peak at 1066 cm(-1) attributed to poly(4-vinylpyridine) (P4VP) which could be used as an intrinsic IS for precise SERS quantification...
July 19, 2017: Analyst
https://www.readbyqxmd.com/read/28718615/development-of-nanofiber-sponges-containing-nerve-guidance-conduit-for-peripheral-nerve-regeneration-in-vivo
#2
Binbin Sun, Zi-Fei Zhou, Tong Wu, Weiming Chen, Dawei Li, Hao Zheng, Hany El-Hamshary, Salem S Al-Deyab, Xiumei Mo, Yinxian Yu
In the study of hollow nerve guidance conduit (NGC), the dispersion of regenerated axons always confused the researchers. To address this problem, filler-containing NGC was prepared, which showed better effect in the application of nerve tissue engineering. In this study, nanofiber sponges with abundant macro-pores, high porosity, and superior compressive strength were fabricated by electrospinning and freeze-drying. Poly (l-lactic acid-co-ε-caprolactone)/silk fibroin (PLCL/SF) nanofiber sponges were used as filler to prepare three-dimensional nanofiber sponges-containing (NS-containing) NGC...
July 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28715926/microencapsulation-of-anthocyanin-rich-black-soybean-coat-extract-by-spray-drying-using-maltodextrin-gum-arabic-and-skimmed-milk-powder
#3
Ana Kalušević, Steva Lević, Bojan Čalija, Milena Pantić, Miona Belović, Vladimir Pavlović, Branko Bugarski, Jela Milić, Slađana Žilić, Viktor Nedović
Black soybean coat is insufficiently valorised food production waste rich in anthocyanins. The goal of the study was to examine physicochemical properties of spray dried extract of black soybean coat in regard to carrier materials: maltodextrin, gum Arabic, and skimmed milk powder. Maltodextrin and gum Arabic-based microparticles were spherical and non-porous while skimmed milk powder-based were irregularly shaped. Low water activity of microparticles (0.31-033), good powders characteristics, high solubility (80...
July 18, 2017: Journal of Microencapsulation
https://www.readbyqxmd.com/read/28715858/biomimetic-nitrogen-doped-titania-nanoparticles-as-a-colorimetric-platform-for-hydrogen-peroxide-detection
#4
Muhammad Nasir, Sajid Rauf, Nawshad Muhammad, Mian Hasnain Nawaz, Aqif Anwar Chaudhry, Muhammad Hamza Malik, Shakir Ahmad Shahid, Akhtar Hayat
Nanoparticles proved a viable alternative to the already used sensing and diagnostics methods due to their low cost, good stability, easy availability and easy synthesis. In the present approach, nitrogen doped titania nanoparticles are prepared through freeze drying method, and subsequently stabilized through ionic liquid. These nanoparticles were characterized through various techniques such as X-ray diffraction (XRD), Fourier transformation infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), BET pore size and surface area analyzer, X-ray photoelectron spectroscopy (XPS) and UV-Visible diffuse reflectance spectroscopy (UV-Vis...
July 6, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28715465/multiplexing-detection-of-igg-against-plasmodium-falciparum-pregnancy-specific-antigens
#5
Ana Maria Fonseca, Llorenç Quinto, Alfons Jiménez, Raquel González, Azucena Bardají, Sonia Maculuve, Carlota Dobaño, Maria Rupérez, Anifa Vala, John J Aponte, Esperanza Sevene, Eusebio Macete, Clara Menéndez, Alfredo Mayor
BACKGROUND: Pregnant women exposed to Plasmodium falciparum generate antibodies against VAR2CSA, the parasite protein that mediates adhesion of infected erythrocytes to the placenta. There is a need of high-throughput tools to determine the fine specificity of these antibodies that can be used to identify immune correlates of protection and exposure. Here we aimed at developing a multiplex-immunoassay to detect antibodies against VAR2CSA antigens. METHODS AND FINDINGS: We constructed two multiplex-bead arrays, one composed of 3 VAR2CSA recombinant-domains (DBL3X, DBL5Ɛ and DBL6Ɛ) and another composed of 46 new peptides covering VAR2CSA conserved and semi-conserved regions...
2017: PloS One
https://www.readbyqxmd.com/read/28703961/effect-of-addition-of-wild-garlic-allium-ursinum-on-the-quality-of-kefirs-from-sheep-s-milk
#6
Agata Znamirowska, Katarzyna Szajnar, Przemysław Rożek, Dorota Kalicka, Piotr Kuźniar, Paweł Hanus, Krzysztof Kotula, Marcin Obirek, Maciej Kluz
BACKGROUND: Sheep’s milk has a high content of total solids, which qualifies it as a very good raw material for the production of fermented milk drinks. Currently, there are commercially produced kefirs and yogurts from sheep’s milk in the countries of the Mediterranean region. The growing interest in the consumption of these products is justified not only by their taste merits, but also because of their health-promoting proper- ties. The aim of the present study was to determine the effect of the addition of 1% of lyophilized wild garlic powder on the properties of kefirs from sheep’s milk...
April 2017: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28703956/optimization-of-mooseer-a-hirtifolium-boiss-dehydration-under-infrared-conditions
#7
Reza Amiri Chayjan, Mosayeb Fealekari
BACKGROUND: In recent years, infrared drying has gained popularity as an alternative drying method for a va- riety of agricultural products. The use of infrared radiation technology in drying agricultural products has several advantages. These may include decreased drying time, high energy efficiency, high-quality finished products and uniform temperature in the product. With intermittent infrared and convection heating of a thick porous material, the drying time can be reduced compared to convection alone, while keeping good food quality and high energy efficiency...
April 2017: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/28700523/dried-blood-spots-combined-with-ultra-high-performance-liquid-chromatography-mass-spectrometry-for-the-quantification-of-the-antipsychotics-risperidone-aripiprazole-pipamperone-and-their-major-metabolites
#8
Camille Tron, Sanne M Kloosterboer, Bart C H van der Nagel, Rixt A Wijma, Bram Dierckx, Gwen C Dieleman, Teun van Gelder, Birgit C P Koch
BACKGROUND: Risperidone, aripiprazole, and pipamperone are antipsychotic drugs frequently prescribed for the treatment of comorbid behavioral problems in children with autism spectrum disorders. Therapeutic drug monitoring (TDM) could be useful to decrease side effects and to improve patient outcome. Dried blood spot (DBS) sample collection seems to be an attractive technique to develop TDM of these drugs in a pediatric population. The aim of this work was to develop and validate a DBS assay suitable for TDM and home sampling...
August 2017: Therapeutic Drug Monitoring
https://www.readbyqxmd.com/read/28698616/barcode-its2-a-useful-tool-for-identifying-trachelospermum-jasminoides-and-a-good-monitor-for-medicine-market
#9
Ning Yu, Yu-Long Wei, Xin Zhang, Ning Zhu, Yan-Li Wang, Yue Zhu, Hai-Ping Zhang, Fen-Mei Li, Lan Yang, Jia-Qi Sun, Ai-Dong Sun
Trachelospermum jasminoides is commonly used in traditional Chinese medicine. However, the use of the plant's local alternatives is frequent, causing potential clinical problems. The T. jasminoides sold in the medicine market is commonly dried and sliced, making traditional identification methods difficult. In this study, the ITS2 region was evaluated on 127 sequences representing T. jasminoides and its local alternatives according to PCR and sequencing rates, intra- and inter-specific divergences, secondary structure, and discrimination capacity...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28693324/zno-nanoparticle-fortified-highly-permeable-carbon-silica-monoliths-as-a-flow-through-media
#10
Srujan Singh, Kunal Mondal, Ashutosh Sharma
We demonstrate a facile one-pot synthesis of porous "flow-through" ZnO nanoparticle impregnated carbon/silica monoliths with high mechanical strength and interconnected end-to-end pores decorated with functional and catalytic nanoparticles. The materials and conditions for the synthesis were tailored to achieve the desired properties of high mechanical strength, good flow-through permeability, and crack-free morphology. Monoliths were prepared from a resorcinol formaldehyde rout but with the addition of tetraethyl-orthosilicate and a metal oxide precursor, ZnCl2...
July 10, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28687391/using-a-bacterial-fucose-rich-polysaccharide-as-encapsulation-material-of-bioactive-compounds
#11
Sofia C Lourenço, Cristiana A V Torres, Daniela Nunes, Paulo Duarte, Filomena Freitas, Maria A M Reis, Elvira Fortunato, Margarida Moldão-Martins, Luísa Beirão da Costa, Vítor D Alves
The potential of a bacterial exopolysaccharide named FucoPol, produced by the bacterium Enterobacter A47, as encapsulation matrix was explored. Spherical capsules with a smooth surface were produced by spray drying. The obtained microcapsules had average diameters ranging from 0.5 to 26.7μm and presented thin walls (thickness from 222 to 1094nm). The capsules were loaded with two bioactive compounds: gallic acid (GA) and oregano essential oil (OEO). Both bioactive materials were encapsulated in FucoPol particles, retaining their antioxidant activity after the drying process...
July 5, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28686961/towards-substrate-independent-age-estimation-of-blood-stains-based-on-dimensionality-reduction-and-k-nearest-neighbor-classification-of-absorbance-spectroscopic-data
#12
Tommy Bergmann, Florian Heinke, Dirk Labudde
The age determination of blood traces provides important hints for the chronological assessment of criminal events and their reconstruction. Current methods are often expensive, involve significant experimental complexity and often fail to perform when being applied to aged blood samples taken from different substrates. In this work an absorption spectroscopy-based blood stain age estimation method is presented, which utilizes 400-640nm absorption spectra in computation. Spectral data from 72 differently aged pig blood stains (2h to three weeks) dried on three different substrate surfaces (cotton, polyester and glass) were acquired and the turnover-time correlations were utilized to develop a straightforward age estimation scheme...
June 8, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28673095/screening-of-ionically-crosslinked-chitosan-tripolyphosphate-microspheres-using-plackett-burman-factorial-design-for-the-treatment-of-intrapocket-infections
#13
Sarita Kumari Yadav, Gayasuddin Khan, Monika Bansal, Harsh Vardhan, Brahmeshwar Mishra
OBJECTIVE: Application of Plackett-Burman factorial design to investigate the effect of processing factors in the fabrication of ionically crosslinked chitosan-tripolyphosphate (CS-TPP) microspheres. SIGNIFICANCE: Microspheres were screened and optimized to provide maximum process yield (PY), encapsulation efficiency (EE), and time for 80% drug release (T80%) and minimum burst and particles size (PS), for successful application in periodontitis. METHODS: Processing factors viz...
July 4, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28670816/liquid-chromatographic-method-for-the-determination-of-caffeoylquinic-acid-derivates-in-hieracium-pilosella-l
#14
Larisa Borisova-Jan, Dick Fransson, Per Claeson, Robert Burman
INTRODUCTION: Hieracium pilosella L. is a medicinal plant encountered in Europe in traditional herbal medicinal products. Caffeoylquinic (ortho-dihydroxycinnamic) acid derivatives are characteristic constituents used as analytical markers in the quality control of the herbal material. Until now, the caffeoylquinic acid derivatives have been assayed using a colorimetric method according to the French Pharmacopoeia. OBJECTIVE: To develop an HPLC-method for quantification of caffeoylquinic acid derivatives in H...
July 2, 2017: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/28664786/combining-dispersed-particle-extraction-with-dried-droplet-laser-ablation-icp-ms-for-determining-platinum-in-airborne-particulate-matter
#15
Winfried Nischkauer, Andrei Izmer, Marie-Alexandra Neouze, Frank Vanhaecke, Andreas Limbeck
A combination of analyte pre-concentration using dispersed particle extraction (DPE) and dried-droplet laser ablation inductively coupled mass spectrometry (LA-ICP-MS) was developed with the aim to quantify Pt and Pd in urban particulate matter with an aerodynamic diameter ≤2.5 µm (PM2.5). The PM2.5 aerosol was collected on cellulose ester filters during a sampling period of three days, with sampling intervals of 4 h only. Each of the filters was chemically digested, and the resulting solution was pre-concentrated using DPE...
July 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/28653139/spreading-proliferation-and-differentiation-of-human-dental-pulp-stem-cells-on-chitosan-scaffolds-immobilized-with-rgd-or-fibronectin
#16
Farzin Asghari Sana, Merve Çapkın Yurtsever, Gökçe Kaynak Bayrak, Ekin Özge Tunçay, Arlin S Kiremitçi, Menemşe Gümüşderelioğlu
Nowadays, human dental pulp stem cells (hDPSCs) became more attractive for therapeutic purposes because of their high proliferation and differentiation potential. Thus, coupling the desired cellular characteristics of hDPSCs with good biomaterial properties of the chitosan scaffolds provide an interesting approach for tissue engineering applications. On the other hand, scaffold surface modification is also needed to promote stem cell adhesion since chitosan lacks adhesion motifs to support direct cell anchorage...
June 26, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28648083/temporal-signal-pattern-recognition-in-mass-spectrometry-a-method-for-rapid-identification-and-accurate-quantification-of-biomarkers-for-inborn-errors-of-metabolism-with-quality-assurance
#17
Alicia DiBattista, Nathan McIntosh, Monica Lamoureux, Osama Y Al-Dirbashi, Pranesh Chakraborty, Philip Britz-McKibbin
Mass spectrometry (MS)-based metabolomic initiatives that use conventional separation techniques are limited by low sample throughput and complicated data processing that contribute to false discoveries. Herein, we introduce a new strategy for unambiguous identification and accurate quantification of biomarkers for inborn errors of metabolism (IEM) from dried blood spots (DBS) with quality assurance. A multiplexed separation platform based on multisegment injection-capillary electrophoresis-mass spectrometry (MSI-CE-MS) was developed to provide comparable sample throughput to flow injection analysis-tandem MS (FIA-MS/MS) but with greater selectivity as required for confirmatory testing and discovery-based metabolite profiling of volume-restricted biospecimens...
July 11, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28639052/gentamicin-loaded-polysaccharide-membranes-for-prevention-and-treatment-of-post-operative-wound-infections-in-the-skeletal-system
#18
Urszula Cibor, Małgorzata Krok-Borkowicz, Monika Brzychczy-Włoch, Łucja Rumian, Krzysztof Pietryga, Dominika Kulig, Wojciech Chrzanowski, Elżbieta Pamuła
PURPOSE: To develop polysaccharide-based membranes that allow controlled and localized delivery of gentamicin for the treatment of post-operative bone infections. METHODS: Membranes made of gellan gum (GUM), sodium alginate (ALG), GUM and ALG crosslinked with calcium ions (GUM + Ca and ALG + Ca, respectively) as well as reference collagen (COL) were produced by freeze-drying. Mechanical properties, drug release, antimicrobial activity and cytocompatibility of the membranes were assessed...
June 21, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28622370/evaluation-of-the-performance-of-abbott-m2000-and-roche-cobas-ampliprep-cobas-taqman-assays-for-hiv-1-viral-load-determination-using-dried-blood-spots-and-dried-plasma-spots-in-kenya
#19
Clement Zeh, Kenneth Ndiege, Seth Inzaule, Rebecca Achieng, John Williamson, Joy Chih-Wei Chang, Dennis Ellenberger, John Nkengasong
BACKGROUND: Routine HIV viral load testing is not widely accessible in most resource-limited settings, including Kenya. To increase access to viral load testing, alternative sample types like dried blood spots (DBS), which overcome the logistic barriers associated with plasma separation and cold chain shipment need to be considered and evaluated. The current study evaluated matched dried blood spots (DBS) and dried plasma spots (DPS) against plasma using the Abbott M 2000 (Abbott) and Roche Cobas Ampliprep/Cobas TaqMan (CAP/CTM) quantitative viral load assays in western Kenya...
2017: PloS One
https://www.readbyqxmd.com/read/28610378/type-designations-and-taxonomic-remarks-for-nearctic-sap-beetles-in-the-subfamily-carpophilinae-erichson-coleoptera-nitidulidae
#20
Gareth S Powell, Andrew R Cline
The subfamily Carpophilinae, in particular the genus Carpophilus Stephens, represents one of the most speciose lineages within Nitidulidae. The subfamily is comprised of more than 250 described species that are found worldwide in every habitable region, and have been transported by man in stored products to remote islands and archipelagos (Ewing & Cline 2005; Parsons 1943). The ubiquitous Carpophilus dimidiatus (L.) is an example of a cosmopolitan species that has been reported from every continent except Antarctica, but likely has been transported there as well...
May 16, 2017: Zootaxa
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