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tula-2

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https://www.readbyqxmd.com/read/27765766/tula-2-t-cell-ubiquitin-ligand-2-inhibits-the-platelet-fc-receptor-for-igg-iia-fc%C3%AE-riia-signaling-pathway-and-heparin-induced-thrombocytopenia-in-mice
#1
Yuhang Zhou, Shaji Abraham, Stephanie Renna, Leonard C Edelstein, Carol A Dangelmaier, Alexander Y Tsygankov, Satya P Kunapuli, Paul F Bray, Steven E McKenzie
OBJECTIVE: The objective of this study is to investigate the role of T-cell ubiquitin ligand-2 (TULA-2) in the platelet Fc receptor for IgG IIA (FcγRIIA) pathway and in the pathogenesis of heparin-induced thrombocytopenia (HIT). APPROACH AND RESULTS: HIT is a life-threatening thrombotic disease in which IgG antibodies against the heparin-platelet factor 4 complex activate platelets via FcγRIIA. We reported previously differential expression of TULA-2 in human population was linked to FcγRIIA responsiveness...
October 20, 2016: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/27609517/tula-2-protein-phosphatase-suppresses-activation-of-syk-through-the-gpvi-platelet-receptor-for-collagen-by-dephosphorylating-tyr-p-346-a-regulatory-site-of-syk
#2
Kevin Reppschläger, Jeanne Gosselin, Carol A Dangelmaier, Dafydd H Thomas, Nick Carpino, Steven E McKenzie, Satya P Kunapuli, Alexander Y Tsygankov
Protein-tyrosine phosphatase TULA-2 has been shown to regulate receptor signaling in several cell types, including platelets. Platelets are critical for maintaining vascular integrity; this function is mediated by platelet aggregation in response to recognition of the exposed basement membrane collagen by the GPVI receptor, which is non-covalently associated with the signal-transducing FcRγ polypeptide chain. Our previous studies suggested that TULA-2 plays an important role in negatively regulating signaling through GPVI-FcRγ and indicated that the tyrosine-protein kinase Syk is a key target of the regulatory action of TULA-2 in platelets...
October 21, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26584182/regulation-of-early-steps-of-gpvi-signal-transduction-by-phosphatases-a-systems-biology-approach
#3
Joanne L Dunster, Francoise Mazet, Michael J Fry, Jonathan M Gibbins, Marcus J Tindall
We present a data-driven mathematical model of a key initiating step in platelet activation, a central process in the prevention of bleeding following Injury. In vascular disease, this process is activated inappropriately and causes thrombosis, heart attacks and stroke. The collagen receptor GPVI is the primary trigger for platelet activation at sites of injury. Understanding the complex molecular mechanisms initiated by this receptor is important for development of more effective antithrombotic medicines. In this work we developed a series of nonlinear ordinary differential equation models that are direct representations of biological hypotheses surrounding the initial steps in GPVI-stimulated signal transduction...
November 2015: PLoS Computational Biology
https://www.readbyqxmd.com/read/26516227/anti-mir-148a-regulates-platelet-fc%C3%AE-riia-signaling-and-decreases-thrombosis-in-vivo-in-mice
#4
Yuhang Zhou, Shaji Abraham, Pierrette Andre, Leonard C Edelstein, Chad A Shaw, Carol A Dangelmaier, Alexander Y Tsygankov, Satya P Kunapuli, Paul F Bray, Steven E McKenzie
Fc receptor for IgG IIA (FcγRIIA)-mediated platelet activation is essential in heparin-induced thrombocytopenia (HIT) and other immune-mediated thrombocytopenia and thrombosis disorders. There is considerable interindividual variation in platelet FcγRIIA activation, the reasons for which remain unclear. We hypothesized that genetic variations between FcγRIIA hyper- and hyporesponders regulate FcγRIIA-mediated platelet reactivity and influence HIT susceptibility. Using unbiased genome-wide expression profiling, we observed that human hyporesponders to FcγRIIA activation showed higher platelet T-cell ubiquitin ligand-2 (TULA-2) mRNA expression than hyperresponders...
December 24, 2015: Blood
https://www.readbyqxmd.com/read/25047644/members-of-the-novel-ubash3-sts-tula-family-of-cellular-regulators-suppress-t-cell-driven-inflammatory-responses-in-vivo
#5
Tiffanny N Newman, Elisabetta Liverani, Elitza Ivanova, Gian L Russo, Nick Carpino, Doina Ganea, Fayez Safadi, Satya P Kunapuli, Alexander Y Tsygankov
The UBASH3/STS/TULA family consists of two members sharing substantial homology and a similar multi-domain architecture, which includes a C-terminal histidine phosphatase domain capable of dephosphorylating phosphotyrosine-containing substrates. TULA-family proteins act as downregulators of receptor-induced activation in several cell types, including T cells and platelets. Deletion of both family members in mice has been shown to result in hyperresponsiveness of T cells to T-cell receptor (TCR)/CD3 complex engagement, but little is known about the biological consequences of double knockout (dKO) and especially of either single KO (sKO)...
November 2014: Immunology and Cell Biology
https://www.readbyqxmd.com/read/23149425/tula-2-a-novel-histidine-phosphatase-regulates-bone-remodeling-by-modulating-osteoclast-function
#6
Steven H Back, Naga Suresh Adapala, Mary F Barbe, Nick C Carpino, Alexander Y Tsygankov, Archana Sanjay
Bone is a dynamic tissue that depends on the intricate relationship between protein tyrosine kinases (PTK) and protein tyrosine phosphatases (PTP) for maintaining homeostasis. PTKs and PTPs act like molecular on and off switches and help modulate differentiation and the attachment of osteoclasts to bone matrix regulating bone resorption. The protein T cell ubiquitin ligand-2 (TULA-2), which is abundantly expressed in osteoclasts, is a novel histidine phosphatase. Our results show that of the two family members, only TULA-2 is expressed in osteoclasts and that its expression is sustained throughout the course of osteoclast differentiation, suggesting that TULA-2 may play a role during early as well late stages of osteoclast differentiation...
April 2013: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/22689384/tula-family-proteins-a-new-class-of-cellular-regulators
#7
REVIEW
Alexander Y Tsygankov
Proteins of the UBASH3/STS/TULA family recently emerged as potent regulators of cellular functions. They are characterized by a unique architecture, featuring at least three functional domains. One of them is a histidine phosphatase domain, which mediates the protein tyrosine phosphatase activity of these proteins. Recent studies demonstrated that UBASH3/STS/TULA-family proteins play a key role in down-regulating receptor-mediated signal transduction and physiologic responses of T cells and platelets in vitro and in vivo...
January 2013: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/22267732/once-phosphorylated-tyrosines-in-carboxyl-terminus-of-protein-tyrosine-kinase-syk-interact-with-signaling-proteins-including-tula-2-a-negative-regulator-of-mast-cell-degranulation
#8
Rodrigo Orlandini de Castro, Juan Zhang, Jacqueline R Groves, Emilia Alina Barbu, Reuben P Siraganian
Activation of the high affinity IgE-binding receptor (FcεRI) results in the tyrosine phosphorylation of two conserved tyrosines located close to the COOH terminus of the protein-tyrosine kinase Syk. Synthetic peptides representing the last 10 amino acids of the tail of Syk with these two tyrosines either nonphosphorylated or phosphorylated were used to precipitate proteins from mast cell lysates. Proteins specifically precipitated by the phosphorylated peptide were identified by mass spectrometry. These included the adaptor proteins SLP-76, Nck-1, Grb2, and Grb2-related adaptor downstream of Shc (GADS) and the protein phosphatases SHIP-1 and TULA-2 (also known as UBASH3B or STS-1)...
March 9, 2012: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/20670933/determination-of-the-substrate-specificity-of-protein-tyrosine-phosphatase-tula-2-and-identification-of-syk-as-a-tula-2-substrate
#9
Xianwen Chen, Lige Ren, Soochong Kim, Nicholas Carpino, James L Daniel, Satya P Kunapuli, Alexander Y Tsygankov, Dehua Pei
TULA-1 (UBASH3A/STS-2) and TULA-2 (p70/STS-1) represent a novel class of protein-tyrosine phosphatases. Previous studies suggest that TULA-2 is sequence-selective toward phosphotyrosyl (Tyr(P)) peptides. In this work the substrate specificity of TULA-1 and -2 was systematically evaluated by screening a combinatorial Tyr(P) peptide library. Although TULA-1 showed no detectable activity toward any of the Tyr(P) peptides in the library, TULA-2 recognizes two distinct classes of Tyr(P) substrates. On the N-terminal side of Tyr(P), the class I substrates contain a proline at the Tyr(P)-1 position, a hydrophilic residue at the Tyr(P)-2 position, and aromatic hydrophobic residues at positions Tyr(P)-3 and beyond...
October 8, 2010: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/20585042/a-novel-histidine-tyrosine-phosphatase-tula-2-associates-with-syk-and-negatively-regulates-gpvi-signaling-in-platelets
#10
Dafydd H Thomas, Todd M Getz, Tiffanny N Newman, Carol A Dangelmaier, Nick Carpino, Satya P Kunapuli, Alexander Y Tsygankov, James L Daniel
T-cell ubiquitin ligand-2 (TULA-2) is a recently discovered histidine tyrosine phosphatase thought to be ubiquitously expressed. In this work, we have investigated whether TULA-2 has a key role in platelet glycoprotein VI (GPVI) signaling. This study indicates that TULA-2 is expressed in human and murine platelets and is able to associate with Syk and dephosphorylate it. Ablation of TULA-2 resulted in hyperphosphorylation of Syk and its downstream effector phospholipase C-γ2 as well as enhanced GPVI-mediated platelet functional responses...
October 7, 2010: Blood
https://www.readbyqxmd.com/read/19585081/tula-family-proteins-an-odd-couple
#11
REVIEW
Alexander Y Tsygankov
Two members of the TULA family (TULA/STS-2/UBASH3A and TULA-2/STS-1/UBASH3B) recently emerged as novel regulators of several cellular functions. The degree of structural similarity between the TULA-family proteins is typical for proteins that belong to the same family. Furthermore, the experiments with knockout mice lacking these proteins may be interpreted as suggesting that functions of TULA-family proteins in T lymphocytes overlap. At the same time, TULA and TULA-2 exhibit clear functional dissimilarities, starting with the finding that a conserved phosphatase domain present in both proteins exhibits remarkable differences in enzymatic activity; TULA-2 is an active phosphatase capable of dephosphorylating multiple tyrosine-phosphorylated proteins, whereas the phosphatase activity of TULA is extremely low...
September 2009: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/18189269/tula-proteins-regulate-activity-of-the-protein-tyrosine-kinase-syk
#12
Rachana Agrawal, Nick Carpino, Alexander Tsygankov
TULA belongs to a two-member family: TULA (STS-2) is a lymphoid protein, whereas STS-1/TULA-2 is expressed ubiquitously. TULA proteins were implicated in the regulation of signaling mediated by protein tyrosine kinases (PTKs). The initial experiments did not fully reveal the molecular mechanism of these effects, but suggested that both TULA proteins act in a similar fashion. It was shown recently that STS-1/TULA-2 dephosphorylates PTKs. In this study, we analyzed the effects of TULA proteins on Syk, a PTK playing an important role in lymphoid signaling...
June 1, 2008: Journal of Cellular Biochemistry
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