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https://www.readbyqxmd.com/read/29729730/dual-functional-extracellular-recording-using-a-light-addressable-potentiometric-sensor-for-bitter-signal-transduction
#1
Liping Du, Jian Wang, Wei Chen, Luhang Zhao, Chunsheng Wu, Ping Wang
This paper presents a dual functional extracellular recording biosensor based on a light-addressable potentiometric sensor (LAPS). The design and fabrication of this biosensor make it possible to record both extracellular membrane potential changes and ATP release from a single taste bud cell for the first time. For detecting ATP release, LAPS chip was functionalized with ATP-sensitive DNA aptamer by covalent immobilization. Taste bud cells isolated from rat were cultured on LAPS surface. When the desired single taste bud cell was illuminated by modulated light, ATP release from single taste bud cells can be measured by recording the shifts of bias voltage-photocurrent curves (I-V curves) when the LAPS chip is working in discrete mode...
August 31, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29723807/the-molecular-selectivity-of-unc3866-inhibitor-for-polycomb-cbx7-protein-from-molecular-dynamics-simulation
#2
Hui Liu, Zhuoming Li, Lang Li
Polycomb CBX proteins regulate gene expression by targeting Polycomb repressive complex 1 (PRC1) to sites of H3K27me3 via their chromodomains, which plays a key role in the development of numerous cancers. UNC3866, is a recently reported peptide-based inhibitor of the methyllysine (Kme) reading function of CBX chromodomains (CBX2, 4 and 6-8). The previous experiments showed that UNC3866 bound the chromodomains of CBX7 strongly, with ∼20-fold selectivity over other CBX chromodomains. However, the potential mechanism of UNC3866 preferentially binding to CBX7 is still unknown...
April 10, 2018: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/29660499/modulating-membrane-fluidity-corrects-batten-disease-phenotypes-in-vitro-and-in-vivo
#3
Mark L Schultz, Luis Tecedor, Elena Lysenko, Shyam Ramachandran, Colleen S Stein, Beverly L Davidson
The neuronal ceroid lipofuscinoses are a class of inherited neurodegenerative diseases characterized by the accumulation of autofluorescent storage material. The most common neuronal ceroid lipofuscinosis has juvenile onset with rapid onset blindness and progressive degeneration of cognitive processes. The juvenile form is caused by mutations in the CLN3 gene, which encodes the protein CLN3. While mouse models of Cln3 deficiency show mild disease phenotypes, it is apparent from patient tissue- and cell-based studies that its loss impacts many cellular processes...
April 13, 2018: Neurobiology of Disease
https://www.readbyqxmd.com/read/29587860/roles-of-astrocytic-connexin-43-hemichannels-and-gap-junctions-in-oxygen-glucose-deprivation-reperfusion-injury-induced-neuroinflammation-and-the-possible-regulatory-mechanisms-of-salvianolic-acid-b-and-carbenoxolone
#4
Xiang Yin, Liangshu Feng, Di Ma, Ping Yin, Xinyu Wang, Shuai Hou, Yulei Hao, Jingdian Zhang, Meiying Xin, Jiachun Feng
BACKGROUND: Glia-mediated neuroinflammation is related to brain injury exacerbation after cerebral ischemia/reperfusion (I/R) injury. Astrocytic hemichannels or gap junctions, which were mainly formed by connexin-43, have been implicated in I/R damage. However, the exact roles of astrocytic hemichannels and gap junction in neuroinflammatory responses induced by I/R injury remain unknown. METHODS: Primary cultured astrocytes were subjected to OGD/R injury, an in vitro model of I/R injury...
March 27, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29554535/plasma-c-reactive-protein-levels-in-bipolar-depression-during-cyclooxygenase-2-inhibitor-combination-treatment
#5
David Edberg, Debra Hoppensteadt, Amanda Walborn, Jawed Fareed, James Sinacore, Angelos Halaris
Immune system activation and neuroinflammation appear to play a key role in the pathophysiology and treatment of bipolar depression (BDD). This study is the first to analyze blood levels of the pro-inflammatory biomarker C-reactive protein (CRP) in bipolar disorder patients treated with the cyclooxygenase-2 inhibitor, celecoxib (CBX). In this double-blind study, 47 consenting patients with BDD were randomized to receive either escitalopram (10 mg twice/day) + CBX (200 mg twice/day), or escitalopram (10 mg twice/day) + placebo (twice/day)...
February 13, 2018: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/29484398/pannexin-1-is-involved-in-neuronal-apoptosis-and-degeneration-in-experimental-intracerebral-hemorrhage-in-rats
#6
Linqiang Zhou, Chenglin Liu, Zhong Wang, Haitao Shen, Zunjia Wen, Dongdong Chen, Qing Sun, Gang Chen
Pannexins serve an important role in the regulation of extracellular neuronal regenerative currents and cellular signal transduction of glial cells; however, the effects of pannexins in various cerebrovascular diseases have not been reported. The present study focused on the expression and influence of pannexins in a rat model of intracerebral hemorrhage (ICH), and confirmed that pannexins (including Pannexin‑1, Pannexin‑2 and Pannexin‑3) are expressed in rat brain tissues. However, only the expression of Pannexin‑1 was significantly increased and peaked 48 h post‑ICH...
April 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29459770/polycomb-protein-family-member-cbx7-regulates-intrinsic-axon-growth-and-regeneration
#7
Run-Shan Duan, Gang-Bin Tang, Hong-Zhen Du, Yi-Wen Hu, Pei-Pei Liu, Ya-Jie Xu, Yu-Qiang Zeng, Shuang-Feng Zhang, Rui-Ying Wang, Zhao-Qian Teng, Chang-Mei Liu
Neurons in the central nervous system (CNS) lose their intrinsic ability and fail to regenerate, but the underlying mechanisms are largely unknown. Polycomb group (PcG) proteins, which include PRC1 and PRC2 complexes function as gene repressors and are involved in many biological processes. Here we report that PRC1 components (polycomb chromobox (CBX) 2, 7, and 8) are novel regulators of axon growth and regeneration. Especially, knockdown of CBX7 in either embryonic cortical neurons or adult dorsal root ganglion (DRG) neurons enhances their axon growth ability...
February 19, 2018: Cell Death and Differentiation
https://www.readbyqxmd.com/read/29456683/carbenoxolone-inhibits-mechanical-stress-induced-osteogenic-differentiation-of-mesenchymal-stem-cells-by-regulating-p38-mapk-phosphorylation
#8
Shenglong Li, Jing Wang, Yudi Han, Xiaoteng Li, Changjian Liu, Zhengshuai Lv, Xiuhui Wang, Xin Tang, Zhe Wang
The aim of the present study was to explore the effects of pannexin1 (Px1) protein channels on osteogenic differentiation of mesenchymal stem cells (MSCs) under mechanical stress stimulation. MSCs were isolated from Sprague Dawley rats (3 weeks old, weighing 100-120 g) and cultured in vitro . A safe concentration of carbenoxolone was determined (CBX, an inhibitor of Px1 channels; 100 µM) on MSCs using the Cell Counting Kit-8 (CCK8) method. MSCs were divided into 6 groups: Control, stress (4,000 µ strain), and stress following 3, 6, 12, and 24 h pretreatment with CBX...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29453024/simultaneous-determination-of-ibuprofen-and-its-metabolites-in-complex-equine-urine-matrices-by-gc-ei-ms-in-excretion-study-in-view-of-doping-control
#9
Emilia Waraksa, Marzena Wójtowicz-Zawadka, Dorota Kwiatkowska, Anna Jarek, Anna Małkowska, Robert Wrzesień, Jacek Namieśnik
A novel assay for the simultaneous determination of ibuprofen (IBU) and its four probable metabolites, 1-hydroxyibuprofen (1-OH IBU), 2-hydroxyibuprofen (2-OH IBU), 3-hydroxyibuprofen (3-OH IBU) and carboxyibuprofen (CBX IBU) in equine urine samples with the application of Gas Chromatography-Electron Ionization-Mass Spectrometry (GC-EI-MS) has been developed and elaborated. The new approach for sample preparation including minimizing matrix effects by the application of weak cation exchange solid-phase extraction together with strong cation exchange solid-phase extraction has been applied...
April 15, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29381334/3-carboxybenzoboroxole-functionalized-polyethylenimine-modified-magnetic-graphene-oxide-nanocomposites-for-human-plasma-glycoproteins-enrichment-under-physiological-conditions
#10
Qiong Wu, Bo Jiang, Yejing Weng, Jianxi Liu, Senwu Li, Yechen Hu, Kaiguang Yang, Zhen Liang, Lihua Zhang, Yukui Zhang
Boronate affinity materials have been successfully used for the selective recognition of glycoproteins. However, by such materials, the large-scale glycoproteins enrichment from human plasma under physiological conditions is rarely reported. In this work, 3-carboxybenzoboroxole (CBX) functionalized polyethylenimine (PEI) modified magnetic graphene oxide nanocomposites were synthesized. Benefitting from the low pKa value of CBX (∼6.9) and PEI dendrimer-assisted multivalent binding, the Freundlich constant (KF ) for the adsorption of horseradish peroxidase (HRP) was 3...
February 20, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29353443/role-of-fluorescent-in-situ-hybridization-cholangioscopic-biopsies-and-eus-fna-in-the-evaluation-of-biliary-strictures
#11
Christian Brooks, Valerie Gausman, Chanthel Kokoy-Mondragon, Khushboo Munot, Sunil P Amin, Amit Desai, Claudine Kipp, John Poneros, Amrita Sethi, Frank G Gress, Michel Kahaleh, Vundavalli V Murty, Reem Sharaiha, Tamas A Gonda
BACKGROUND AND AIMS: Our goal was to compare the diagnostic accuracy of FISH in the detection of malignancy compared with other standard diagnostic modalities, including brush cytology and biopsy specimens over a 10-year period of prospective data collection. METHODS: We conducted a review of all consecutive biliary strictures evaluated between 2006 and 2016. Patients with a final pathologic diagnosis or conclusive follow-up were included. We evaluated the performance of FISH polysomy (CEP 3, 7, and 17) and 9p21 deletion as well as cholangioscopic biopsy (CBx) and EUS-FNA...
March 2018: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29285645/analysis-of-ibuprofen-and-its-main-metabolites-in-roots-shoots-and-seeds-of-cowpea-vigna-unguiculata-l-walp-using-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry-uptake-metabolism-and-translocation
#12
Yolanda Picó, Rodrigo Alvarez-Ruiz, Leonard Wijaya, Ahmed Alfarhan, Mohammed Alyemeni, Damià Barceló
A liquid chromatography quadruple time-of-flight mass spectrometry (LC-QqTOF-MS/MS) method was developed for simultaneous quantitative analysis of ibuprofen (IBU), 1- and 2-hydroxyibuprofen (1-OH IBU and 2-OH IBU), and carboxyibuprofen (CBX IBU) while preserving the ability of the instrument to get precursor and product ion mass spectra of non-target compounds. The trigger was the precursor ions reaching 100 cps intensity. Sample preparation was carried out by ultrasound solid-liquid extraction with methanol as extraction solvent at pH < 2 followed by solid-phase extraction (SPE) clean-up using STRATA-X cartridges and methanol as an eluent...
January 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29229426/cbx4-exhibits-oncogenic-activities-in-breast-cancer-via-notch1-signaling
#13
Jing-Sheng Zeng, Zhen-Dong Zhang, Li Pei, Zhi-Zhu Bai, Yong Yang, Hong Yang, Qiu-Hong Tian
Polycomb chromobox (CBX) proteins are involved in gene silencing to function as oncogenes or tumor suppressors through the polycomb repressive complex (PRC1). CBX4 has been implicated in the progression of human cancers, but its role and clinical significance in breast cancer remain unclear. Here, we show that CBX4 is up-regulated in breast cancer and exerts oncogenic activities via miR-137-mediated activation of Notch1 signaling pathway. CBX4 expression was increased in breast cancer, compared with the nontumorous tissues...
February 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29221793/pannexin-1-channel-dysfunction-in-the-medial-prefrontal-cortex-mediates-depressive-like-behaviors-induced-by-chronic-social-defeat-stress-and-administration-of-mefloquine-in-mice
#14
Ming Ni, Jin-Gang He, Hai-Yun Zhou, Xiao-Jia Lu, Yuan-Lang Hu, Li Mao, Fang Wang, Jian-Guo Chen, Zhuang-Li Hu
Mefloquine (MFQ) is widely used for the treatment of malaria clinically. Apart from antimalarial effect, psychiatric side effects such as depression and anxiety of MFQ have been reported. Interestingly, MFQ is also known as a broad-spectrum pannexin-1 (Panx1) inhibitor. Panx1 is a new gap junction channel expressed in the brain which mediates efflux of adenosine triphosphate (ATP). Although exogenous ATP has been known to produce a potential antidepressant-like effect, little is known about the role of Panx1 in pathophysiology of depression, especially the depression induced by administration of MFQ...
December 5, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/29190923/prognostic-values-of-distinct-cbx-family-members-in-breast-cancer
#15
Yuan-Ke Liang, Hao-Yu Lin, Chun-Fa Chen, De Zeng
Chromobox (CBX) family proteins are canonical components in polycomb repressive complexes 1 (PRC1), with epigenetic regulatory function and transcriptionally repressing target genes via chromatin modification. A plethora of studies have highlighted the function specifications among CBX family members in various cancer, including lung cancer, colon cancer and breast cancer. Nevertheless, the functions and prognostic roles of distinct CBX family members in breast cancer (BC) remain elusive. In this study, we reported the prognostic values of CBX family members in patients with BC through analysis of a series of databases, including CCLE , ONCOMINE , Xena Public Data Hubs, and Kaplan-Meier plotter...
November 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/29124886/cbx3-heterochromatin-protein-1-gamma-is-significantly-upregulated-in-patients-with-non-small-cell-lung-cancer
#16
Shih-Chieh Chang, Yi-Chun Lai, Yen-Chung Chen, Nai-Kuan Wang, Wei-Shu Wang, Jiun-I Lai
AIM: Lung cancer is typically categorized into small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). NSCLC comprises of the majority of lung cancer with a poor prognosis in advanced cases. Transcriptional profiling studies, including microarrays and RNA-sequencing studies, have significantly enriched our knowledge of gene expression patterns in NSCLC. A recent transcriptional profiling study identified high prevalence of CBX3/HP1-gamma upregulation in human NSCLC samples...
November 10, 2017: Asia-Pacific Journal of Clinical Oncology
https://www.readbyqxmd.com/read/29089522/the-polycomb-group-protein-cbx6-is-an-essential-regulator-of-embryonic-stem-cell-identity
#17
Alexandra Santanach, Enrique Blanco, Hua Jiang, Kelly R Molloy, Miriam Sansó, John LaCava, Lluis Morey, Luciano Di Croce
Polycomb group proteins (PcG) are transcriptional repressors that control cell identity and development. In mammals, five different CBX proteins associate with the core Polycomb repressive complex 1 (PRC1). In mouse embryonic stem cells (ESCs), CBX6 and CBX7 are the most highly expressed CBX family members. CBX7 has been recently characterized, but little is known regarding the function of CBX6. Here, we show that CBX6 is essential for ESC identity. Its depletion destabilizes the pluripotency network and triggers differentiation...
November 1, 2017: Nature Communications
https://www.readbyqxmd.com/read/28966948/labelling-halotag-fusion-proteins-with-halotag-ligand-in-living-cells
#18
Huy Nguyen Duc, Xiaojun Ren
HaloTag has been widely used to label proteins in vitro and in vivo (Los et al., 2008). In this protocol, we describe labelling HaloTag-Cbx fusion proteins by HaloTag ligands for live-cell single-molecule imaging (Zhen et al., 2016).
September 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28947664/polycomb-complexes-in-x-chromosome-inactivation
#19
REVIEW
Neil Brockdorff
Identifying the critical RNA binding proteins (RBPs) that elicit Xist mediated silencing has been a key goal in X inactivation research. Early studies implicated the Polycomb proteins, a family of factors linked to one of two major multiprotein complexes, PRC1 and PRC2 (Wang 2001 Nat. Genet.28, 371-375 (doi:10.1038/ng574); Silva 2003 Dev. Cell4, 481-495 (doi:10.1016/S1534-5807(03)00068-6); de Napoles 2004 Dev. Cell7, 663-676 (doi:10.1016/j.devcel.2004.10.005); Plath 2003 Science300, 131-135 (doi:10.1126/science...
November 5, 2017: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/28934256/transcriptome-analysis-of-sex-related-genes-in-the-blood-clam-tegillarca-granosa
#20
COMPARATIVE STUDY
Heng Chen, Guoqiang Xiao, Xueliang Chai, Xingguan Lin, Jun Fang, Shuangshuang Teng
BACKGROUND: Blood clams (Tegillarca granosa) are one of the most commercial shellfish in China and South Asia with wide distribution in Indo-Pacific tropical to temperate estuaries. However, recent data indicate a decline in the germplasm of this species. Furthermore, the molecular mechanisms underpinning reproductive regulation remain unclear and information regarding genetic diversity is limited. Understanding the reproductive biology of shellfish is important in interpreting their embryology development, reproduction and population structure...
2017: PloS One
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