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https://www.readbyqxmd.com/read/27914026/limbal-stem-cell-and-oral-mucosal-epithelial-transplantation-from-ex-vivo-cultivation-in-lscd-induced-rabbits-histology-and-immunologic-study-of-the-transplant-epithelial-sheet
#1
Napaporn Tananuvat, Kanokkan Bumroongkit, Chainarong Tocharusa, Umnat Mevatee, Aphisek Kongkaew, Somsanguan Ausayakhun
PURPOSE: To evaluate the results of cultivated limbal epithelial and oral mucosal epithelial transplantation (CLET and COMET) in limbal stem cell deficiency (LSCD)-induced rabbit model. MATERIALS AND METHODS: Six New Zealand white rabbits were divided into two groups of three rabbits each. Limbal tissue was harvested from the first group, and oral mucosal biopsy was obtained from the second group. The tissues were cultured using an explant technique with amniotic membrane as a substrate and co-culture with the 3T3 fibroblast and air-lifting method...
December 2, 2016: International Ophthalmology
https://www.readbyqxmd.com/read/27888644/myrtol-standardized-affects-mucociliary-clearance
#2
Ying Ying Li, Jing Liu, Chun Wei Li, Somasundaram Subramaniam, Siew Shuen Chao, Feng Gang Yu, Noam A Cohen, Shi Li, Yun Wang
BACKGROUND: Myrtol standardized (Gelomyrtol forte) has been shown to be effective in controlling nasal symptoms of rhinosinusitis by promoting mucociliary clearance. Our aim was to evaluate the short- and long-term effects of myrtol on ciliated columnar cells and goblet cells in an in-vitro setting. METHODS: Nasal epithelial cells were harvested (42 days) from an air-liquid interface (ALI) culture of human nasal epithelial stem/progenitor cells (hNESPCs), which was derived from biopsies of nasal inferior turbinate mucosa...
November 26, 2016: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/27881419/oral-administration-of-a-select-mixture-of-bacillus-probiotics-affects-the-gut-microbiota-and-goblet-cell-function-in-newly-weaned-muc4-resistant-pigs-following-escherichia-coli-challenge
#3
Wei Zhang, Yao-Hong Zhu, Dong Zhou, Qiong Wu, Dan Song, Johan Dicksved, Jiu-Feng Wang
: Structural disruption of the gut microbiota and impaired goblet cell function are collateral etiologic factors in enteric diseases. Low, moderate, or high doses of a Bacillus licheniformis-B. subtilis mixture (BLS-mix) were orally administered to MUC4 resistant piglets for 1 week before the F4-expressing ETEC strain (F4(+) ETEC) challenge. The luminal contents were collected from the mucosa of colon on day 8 after F4(+) ETEC challenge. BLS-mix attenuated E. coli-induced expansion of Bacteroides uniformis, Eubacterium eligens, Acetanaerobacterium and Sporobacter populations...
November 23, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27878831/i%C3%AE%C2%BAbns-induces-muc5ac-expression-in-epithelial-cells-and-causes-airway-hyper-responsiveness-in-murine-asthma-models
#4
M Yokota, T Tamachi, Y Yokoyama, Y Maezawa, H Takatori, A Suto, K Suzuki, K Hirose, K Takeda, H Nakajima
BACKGROUND: In allergic asthma, environmental allergens including house dust mite (HDM) trigger pattern recognition receptors and activate downstream signaling pathways including NF-κB pathways not only in immune cells but also in airway epithelial cells. Recent studies have shown that NF-κB activation is regulated positively or negatively depending on the cellular context by IκBNS (encoded by the gene Nfkbid), one of atypical IκB proteins, in the nucleus. Therefore, we hypothesized that IκBNS expressed in immune cells or epithelial cells is involved in the regulation of asthmatic responses...
November 6, 2016: Allergy
https://www.readbyqxmd.com/read/27876210/conditioned-medium-from-ls-174t-goblet-cells-treated-with-oxyresveratrol-strengthens-tight-junctions-in-caco-2-cells
#5
Dahyun Hwang, HyunA Jo, Seonwook Hwang, Jeong-Keun Kim, In-Ho Kim, Young-Hee Lim
BACKGROUND: Strengthening of intestinal tight junctions provides an effective barrier from the external environment. Goblet cell-derived trefoil factor 3 (TFF3) increases transepithelial resistance by upregulating the expression of tight junction proteins. Oxyresveratrol (OXY) is a hydroxyl-substituted stilbene found in the roots, leaves, stems, and fruit of many plants and known to have various biological activities. In this study, we investigated the strengthening effect of OXY on intestinal tight junctions through stimulation of TFF production in goblet cells...
November 18, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27872714/effects-of-dietary-supplementation-of-organic-acids-and-phytase-on-performance-and-intestinal-histomorphology-of-broilers
#6
Nasibeh Mohammadagheri, Ramin Najafi, Gholamreza Najafi
The present experiment was conducted to evaluate the effects of organic acids and phytase enzyme supplementation on performance and intestinal histomorphology of broilers. The experiment was done in a factorial arrangement 2 × 2 × 2 based on completely randomized design with eight treatments, five replicates with 12 chicks in each until 42 days of age. Diets included natural vinegar (0 and 2%), citric acid (CA; 0.00 and 1.00%) and phytase enzyme (PHY; 0.00 and 500 FTU phytase per kg of feed). One bird from each treatment replicate was randomly selected and slaughtered to evaluate the small intestinal morphology on 42 days of age...
2016: Veterinary Research Forum
https://www.readbyqxmd.com/read/27870288/effect-of-dietary-supplementation-with-clay-based-binders-on-biochemical-and-histopathological-changes-in-organs-of-turkey-fed-with-aflatoxin-contaminated-diets
#7
A O Lala, O L Ajayi, A O Oso, M O Ajao, O O Oni, N Okwelum, O M O Idowu
This study was carried out to investigate the effect of dietary supplementation with molecular or nano-clay binders on biochemical and histopathological examination of organs of turkeys fed diets contaminated with aflatoxin B1. Two hundred and sixteen unsexed 1-day-old British United Turkeys were randomly allotted to nine diets in a 3 × 3 factorial arrangement of diets supplemented with no toxin binder, molecular toxin binder (MTB) and nano-clay toxin binder, each contaminated with 0, 60 and 110 ppb aflatoxin B1 respectively...
December 2016: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/27865862/impaired-mucus-clearance-exacerbates-allergen-induced-type-2-airway-inflammation-in-juvenile-mice
#8
Benedikt Fritzsching, Matthias Hagner, Lu Dai, Sandra Christochowitz, Raman Agrawal, Charlotte van Bodegom, Simone Schmidt, Jolanthe Schatterny, Stephanie Hirtz, Ryan Brown, Michelle Goritzka, Julia Duerr, Zhe Zhou-Suckow, Marcus A Mall
BACKGROUND: Type 2 airway inflammation plays a central role in the pathogenesis of allergen-induced asthma, but the underlying mechanisms remain poorly understood. We recently demonstrated that reduced mucociliary clearance, a characteristic feature of asthma, produces spontaneous type 2 airway inflammation in juvenile Scnn1b-transgenic (Scnn1b-Tg) mice. OBJECTIVE: To determine the role of impaired mucus clearance in the pathogenesis of allergen-induced type 2 airway inflammation and identify cellular sources of the signature cytokine IL-13...
November 16, 2016: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/27862316/cdx2-expression-induced-by-leukocytapheresis-might-be-associated-with-mucosal-healing-in-ulcerative-colitis
#9
Kiichiro Tsuchiya, Ryohei Hayashi, Keita Fukushima, Shuji Hibiya, Nobukatsu Horita, Mariko Negi, Eisaku Itoh, Takumi Akashi, Yoshinobu Eishi, Satoshi Motoya, Yoshiaki Takeuchi, Reiko Kunisaki, Ken Fukunaga, Shiro Nakamura, Naoki Yoshimura, Masakazu Takazoe, Bunei Iizuka, Yasuo Suzuki, Masakazu Nagahori, Mamoru Watanabe
BACKGROUND AND AIMS: Ulcerative colitis (UC) is a chronic inflammatory disease of the colon with an intractable, recurrent course. Although the goal of UC therapy has recently been to target mucosal healing, the molecular mechanism of mucosal healing remains unknown. In this study, we aimed to elucidate the molecular dynamics related to the proliferation and differentiation of intestinal epithelial cells (IECs) during cytapheresis therapy in a short duration. METHODS: Endoscopy was performed in 26 patients with UC in multicentre hospitals, and biopsy specimens were collected from the rectum before and within two weeks after leukocytapheresis (LCAP)...
November 11, 2016: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/27856177/diagnostic-criteria-for-limbal-stem-cell-deficiency-a-systematic-literature-review
#10
REVIEW
Lona Jawaheer, Deepa Anijeet, Kanna Ramaesh
PURPOSE: The diagnosis of limbal stem cell deficiency (LSCD) is often based on clinical manifestations with or without the use of tests to demonstrate the presence of goblet cells or of specific epithelial markers on the corneolimbal surface. This systematic review looks at the various diagnostic methods employed in the diagnosis of LSCD in published interventional studies. DESIGN: Systematic literature review METHODS: We did a systematic search on MEDLINE and PUBMED for articles published in English between January 1, 2003 and December 31, 2013...
November 14, 2016: Survey of Ophthalmology
https://www.readbyqxmd.com/read/27849395/giemsa-based-cytological-assessment-of-area-shape-and-nucleus-cytoplasm-ratio-of-goblet-cells-of-rabbit-bulbar-conjunctiva
#11
M J Doughty
Goblet cells were visualized in impression cytology specimens from bulbar conjunctiva of the rabbit eye using Giemsa staining. Highly magnified images were used to generate outlines of the goblet cells and their characteristic eccentric nuclei. Using sets of 10 cells from 15 cytology specimens, I found that the longest dimension of the goblet cells averaged 16.7 ± 2.3 μm, the shortest dimension averaged 14.4 ± 1.8 μm and the nucleus averaged 6.3 ± 0.8 μm. The goblet cells were ellipsoid in shape and the longest:shortest cell dimension ratio averaged 1...
November 2016: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/27847210/targeted-inhibition-of-six1-attenuates-allergic-airway-inflammation-and-remodeling-in-asthmatic-mice
#12
Zhao-Chuan Yang, Ming-Ji Yi, Yan-Chun Shan, Chong Wang, Ni Ran, Li-Ying Jin, Peng Fu, Xue-Ying Feng, Lei Xu, Zheng-Hai Qu
Asthma is an inflammatory disease of the airways, characterized by lung eosinophilia, mucus hypersecretion by goblet cells and airway hyperresponsiveness to inhaled allergens. The purpose of this study was to evaluate the effects of Six1 on airway inflammation and remodeling and the underlying mechanisms in a murine model of chronic asthma. Female BALB/c mice were randomly divided into four groups: phosphate-buffered saline control, ovalbumin (OVA)-induced asthma group, OVA+siNC and OVA+siSix1. In this mice model, Six1 expression level was significantly elevated in OVA-induced asthma of mice...
November 12, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27836362/intestinal-epithelial-injury-induced-by-maternal-separation-is-protected-by-hydrogen-sulfide
#13
Bo Li, Carol Lee, Zechariah Martin, Xinpei Li, Yuhki Koike, Alison Hock, Elke Zani-Ruttenstock, Augusto Zani, Agostino Pierro
PURPOSE: Oxidative stress has been implicated in the pathogenesis of various neonatal diseases involving the intestine. Hydrogen sulfide (H2S) has been shown to protect against oxidative stress. We hypothesized that administration of sodium hydrosulfide (NaHS), an H2S donor, to neonatal mice can decrease the intestinal epithelial injury associated with maternal separation (MS). METHODS: C57BL/6 mice received either intraperitoneal phosphate buffered saline (PBS; n=10) or NaHS (1mg/kg/day; n=10), followed by MS for 3h daily between postnatal day P5 and P9...
October 26, 2016: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/27832279/artificial-polymeric-scaffolds-as-extracellular-matrix-substitutes-for-autologous-conjunctival-goblet-cell-expansion
#14
Min He, Thomas Storr-Paulsen, Annie L Wang, Chiara E Ghezzi, Siran Wang, Matthew Fullana, Dimitrios Karamichos, Tor P Utheim, Rakibul Islam, May Griffith, M Mirazul Islam, Robin R Hodges, Gary E Wnek, David L Kaplan, Darlene A Dartt
Purpose: We fabricated and investigated polymeric scaffolds that can substitute for the conjunctival extracellular matrix to provide a substrate for autologous expansion of human conjunctival goblet cells in culture. Methods: We fabricated two hydrogels and two silk films: (1) recombinant human collagen (RHC) hydrogel, (2) recombinant human collagen 2-methacryloylxyethyl phosphorylcholine (RHC-MPC) hydrogel, (3) arginine-glycine-aspartic acid (RGD) modified silk, and (4) poly-D-lysine (PDL) coated silk, and four electrospun scaffolds: (1) collagen, (2) poly(acrylic acid) (PAA), (3) poly(caprolactone) (PCL), and (4) poly(vinyl alcohol) (PVA)...
November 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27831916/retrospective-evaluation-of-the-clinical-utility-of-serological-biomarkers-in-chinese-patients-with-inflammatory-bowel-disease-2-year-clinical-experience
#15
Shulan Zhang, Jing Luo, Ji Li, Ziyan Wu, Chaojun Hu, Ping Li, Chuiwen Deng, Fengchun Zhang, Jiaming Qian, Yongzhe Li
BACKGROUND: Antibodies to saccharomyces cerevisiae (ASCA), antibodies to perinuclear anti-neutrophil cytoplasmic (pANCA), pancreatic autoantibodies (PAB) and antibodies against intestinal goblet cells (GAB) are important in diagnosing Crohn's disease (CD) and ulcerative colitis (UC). However, little is known about their diagnostic value in real clinical practice in China. This retrospective study aimed to present our 2-year clinical experience with those biomarkers in diagnosis of CD and UC...
November 10, 2016: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/27830037/goblet-cell-carcinoids-of-the-appendix-tumor-biology-mutations-and-management-strategies
#16
REVIEW
Santosh Shenoy
Malignant neoplasms of the appendix are rare and represent less than 1% of gastrointestinal cancers. Goblet cell carcinoids (GCC) tumors are a distinctive group of heterogeneous appendiceal neoplasm that exhibit unique clinical and pathologic features. This review focuses on the current diagnostic procedures, pathogenesis, possible signaling mechanisms and treatment options for GCC. Perspectives for future research are discussed. The tumor likely arises from pluripotent intestinal epithelial crypt base stem cells...
October 27, 2016: World Journal of Gastrointestinal Surgery
https://www.readbyqxmd.com/read/27826959/inhibitory-effects-of-resveratrol-on-airway-remodeling-by-transforming-growth-factor-%C3%AE-smad-signaling-pathway-in-chronic-asthma-model
#17
Hwa Young Lee, In Kyoung Kim, Hyoung Kyu Yoon, Soon Suk Kwon, Chin Kook Rhee, Sook Young Lee
PURPOSE: Asthma is a chronic airway disease characterized by airway remodeling, leading to a progressive decline in lung function. Therapeutic agents that attenuate airway remodeling can complement the limited effects of traditional glucocorticoids. In this study, we investigated the effect of resveratrol on allergic airway inflammation and remodeling in a murine model of chronic bronchial asthma. METHODS: Peribronchial smooth muscle thickening that developed in mice challenged with a 3-month repeated exposure to ovalbumin (OVA) was used to study airway remodeling...
January 2017: Allergy, Asthma & Immunology Research
https://www.readbyqxmd.com/read/27825108/runx2-a-novel-regulator-for-goblet-cell-differentiation-and-asthma-development
#18
Ning Shi, Jing Zhang, Shi-You Chen
Airway goblet-cell differentiation and related mucus overproduction are critical processes in the development of respiratory diseases, including asthma. The underlying mechanisms, however, are not fully understood. We identified Runt-related transcription factor 2 (Runx2) as a novel regulator for goblet-cell differentiation. Runx2 was up-regulated by 6.4-fold during IL-13-induced goblet-cell differentiation of human bronchial epithelial cells. Knockdown of Runx2 attenuated the IL-13-induced differentiation/mucus production by 67%...
October 17, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27824117/lipoxin-a4-counter-regulates-histamine-stimulated-glycoconjugate-secretion-in-conjunctival-goblet-cells
#19
Robin R Hodges, Dayu Li, Marie A Shatos, Charles N Serhan, Darlene A Dartt
Conjunctival goblet cells synthesize and secrete mucins which play an important role in protecting the ocular surface. Pro-resolution mediators, such as lipoxin A4 (LXA4), are produced during inflammation returning the tissue to homeostasis and are also produced in non-inflamed tissues. The purpose of this study was to determine the actions of LXA4 on cultured human conjunctival goblet cell mucin secretion and increase in intracellular [Ca(2+)] ([Ca(2+)]i) and on histamine-stimulated responses. LXA4 increased mucin secretion and [Ca(2+)]i, and activated ERK1/2 in human goblet cells...
November 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27812173/indoleamine-2-3-dioxygenase-is-not-a-pivotal-regulator-responsible-for-suppressing-allergic-airway-inflammation-through-adipose-derived-stem-cells
#20
Kyu-Sup Cho, Mi-Kyung Park, Sue-Jean Mun, Hee-Young Park, Hak-Sun Yu, Hwan-Jung Roh
BACKGROUND: Although indoleamine 2,3-dioxygenase (IDO)-mediated immune suppression of mesenchymal stem cells (MSCs) has been revealed in septic and tumor microenvironments, the role of IDO in suppressing allergic airway inflammation by MSCs is not well documented. We evaluated the effects of adipose-derived stem cells (ASCs) on allergic inflammation in IDO-knockout (KO) asthmatic mice or asthmatic mice treated with ASCs derived from IDO-KO mice. METHODS AND FINDINGS: ASCs were injected intravenously in wild-type (WT) and IDO-KO asthmatic mice...
2016: PloS One
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