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https://www.readbyqxmd.com/read/28211929/calcium-phosphate-nanoparticles-prepared-from-infusion-fluids-for-stem-cell-transfection-process-optimization-and-cytotoxicity-analysis
#1
Quazi T H Shubhra, Ayako Oyane, Hiroko Araki, Maki Nakamura, Hideo Tsurushima
This is the first study to report the use of infusion fluids for particle-mediated gene delivery with DNA-immobilized calcium phosphate (CaP) nanoparticles (NPs). In conventional CaP systems, CaP NPs are fabricated in labile supersaturated CaP solutions which are prepared from chemical reagents. In the present study, we fabricated CaP NPs via coprecipitation in labile supersaturated CaP solutions that were prepared from infusion fluids (even the water used was of injectable quality) instead of chemical reagents and demonstrated their gene delivery capabilities for the hard to transfect pluripotent stem cell (C3H10T1/2) along with the easy to transfect CHO-K1 cell...
February 17, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28209267/assessment-of-volume-status-and-appropriate-fluid-replenishment-in-the-setting-of-nephrotic-syndrome
#2
Pierluigi Marzuillo, Stefano Guarino, Andrea Apicella, Rosaria Marotta, Vincenzo Tipo, Laura Perrone, Angela La Manna, Giovanni Montini
BACKGROUND: When the permeability of the glomerular filtration barrier increases, leading to proteinuria, nephrotic syndrome (NS) occurs. First episodes or relapses of NS can be concurrent with acute gastroenteritis (AGE) infections. This condition can cause further deterioration of the hypovolemic state, as intravascular water is lost through both AGE-related vomiting/diarrhea and NS-related fluid shifting into the interstitium. In this case report, we wish to raise the issues about the difficult management of children presenting with both NS and AGE...
February 10, 2017: Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28205108/water-assisted-colonoscopy
#3
REVIEW
Sergio Cadoni, Felix W Leung
The current review will attempt to describe the important lessons learned from published randomized controlled trials (RCT) comparing water immersion (WI) or water exchange (WE) techniques with gas insufflation colonoscopy. Air insufflation (AI) to distend the colon to permit visualization and passage through the lumen was developed for diagnostic colonoscopy. When screening colonoscopy was adopted, the same AI method was used. Interval cancers, diagnosed within 3 to 5 years after an index screening colonoscopy, appeared to be linked to low adenoma detection rate (ADR)...
February 15, 2017: Current Treatment Options in Gastroenterology
https://www.readbyqxmd.com/read/28193425/black-tea-volatiles-fingerprinting-by-comprehensive-two-dimensional-gas-chromatography-mass-spectrometry-combined-with-high-concentration-capacity-sample-preparation-techniques-toward-a-fully-automated-sensomic-assessment
#4
Federico Magagna, Chiara Cordero, Cecilia Cagliero, Erica Liberto, Patrizia Rubiolo, Barbara Sgorbini, Carlo Bicchi
Tea prepared by infusion of dried leaves of Camellia sinensis (L.) Kuntze, is the second world's most popular beverage, after water. Its consumption is associated with its chemical composition: it influences its sensory and nutritional quality addressing consumer preferences, and potential health benefits. This study aims to obtain an informative chemical signature of the volatile fraction of black tea samples from Ceylon by applying the principles of sensomics. In particular, several high concentration capacity (HCC) sample preparation techniques were tested in combination with GC×GC-MS to investigate chemical signatures of black tea volatiles...
June 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28192132/flavor-preferences-conditioned-by-nutritive-and-non-nutritive-sweeteners-in-mice
#5
Anthony Sclafani, Karen Ackroff
Recent studies suggest that preferences are conditioned by nutritive (sucrose) but not by non-nutritive (sucralose) sweeteners in mice. Here we compared the effectiveness of nutritive and non-nutritive sweeteners to condition flavor preferences in three mouse strains. Isopreferred sucrose and sucralose solutions both conditioned flavor preferences in C57BL/6J (B6) mice but sucrose was more effective, consistent with its post-oral appetition action. Subsequent experiments compared flavor conditioning by fructose, which has no post-oral appetition effect in B6 mice, and a sucralose+saccharin mixture (SS) which is highly preferred to fructose in 24-h choice tests...
February 9, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28183287/price-elasticity-of-the-demand-for-soft-drinks-other-sugar-sweetened-beverages-and-energy-dense-food-in-chile
#6
Carlos M Guerrero-López, Mishel Unar-Munguía, M Arantxa Colchero
BACKGROUND: Chile is the second world's largest per capita consumer of caloric beverages. Caloric beverages are associated with overweight, obesity and other chronic diseases. The objective of this study is to estimate the price elasticity of demand for soft drinks, other sugar-sweetened beverages and high-energy dense foods in urban areas in Chile in order to evaluate the potential response of households' consumption to changes in prices. METHODS: We used microdata from the VII Family Budget Survey 2012-2013, which collects information on expenditures made by Chilean urban households on items such as beverages and foods...
February 10, 2017: BMC Public Health
https://www.readbyqxmd.com/read/28157816/synthesis-and-characterization-of-%C3%AE-tricalcium-phosphate-derived-from-haliotis-sp-shells
#7
Kyeong-Rok Kang, Zheng-Gang Piao, Jae-Sung Kim, In-A Cho, Min-Ji Yim, Bok-Hee Kim, Ji-Su Oh, Jun Sik Son, Chun Sung Kim, Do Kyung Kim, Sook-Young Lee, Su-Gwan Kim
PURPOSE: To develop a methodology for the synthesis of β-tricalcium phosphate (β-TCP, Ca3(PO4)2) from the shell of Haliotis sp. (abalone shell) and to verify its characterization and biocompatibility. MATERIALS AND METHODS: Calcium oxide (CaO) was synthesized from abalone shell by sintering and was suspended in distilled water to prepare calcium hydroxide (Ca(OH)2). For the synthesis of calcium carbonate (CaCO3), carbon dioxide was used to infuse Ca(OH)2 at pH 7...
February 2, 2017: Implant Dentistry
https://www.readbyqxmd.com/read/28147701/genotoxic-and-chemopreventive-assessment-of-cynara-scolymus-l-aqueous-extract-in-a-human-derived-liver-cell-line
#8
Regiane Pereira da Silva, Laura Vicedo Jacociunas, Raíne Fogliati de Carli, Bianca Regina Ribas de Abreu, Mauricio Lehmann, Juliana da Silva, Alexandre de Barros Falcão Ferraz, Rafael Rodrigues Dihl
Cynara scolymus L., popularly known as artichoke, is consumed as food and used as tea infusions for pharmacological purposes to treat liver dysfunctions and other conditions. Scientific data on the safety and protective effect of artichoke in human-derived liver cells is missing. This study investigated the genotoxic and modulatory effect of a liophilized extract suspended in water of C. scolymus L. leaves. Four extract concentrations (0.62, 1.25, 2.5 and 5.0 mg/mL) were evaluated using the comet assay on human hepatocyte cultures, HepG2 cells...
February 1, 2017: Drug and Chemical Toxicology
https://www.readbyqxmd.com/read/28147245/angiotensin-1-7-administration-attenuates-alzheimer-s-disease-like-neuropathology-in-rats-with-streptozotocin-induced-diabetes-via-mas-receptor-activation
#9
Jin-Liang Chen, Dong-Ling Zhang, Yue Sun, Yu-Xing Zhao, Ke-Xiang Zhao, Die Pu, Qian Xiao
Diabetes mellitus (DM) is associated with cognitive deficits and an increased risk of Alzheimer's disease (AD). Recently, a newly identified heptapeptide of the renin-angiotensin system (RAS), angiotensin-(1-7) [Ang-(1-7)], was found to protect against brain damage. This study investigated the effects of Ang-(1-7) on diabetes-induced cognitive deficits. Sprague-Dawley rats were randomly divided into four groups. Diabetes was induced via single i.p. streptozotocin (STZ) injections. Ten weeks after diabetes induction, rats in each group received an intracerebral-ventricular (ICV) infusion of either vehicle, Ang-(1-7) alone, or Ang-(1-7)+A779 daily for two weeks...
January 29, 2017: Neuroscience
https://www.readbyqxmd.com/read/28144964/pharmacokinetics-and-protein-binding-of-mpt0b292
#10
Yu-En Tien, Chan-Jung Li, Jing-Ping Liou, Jang-Yang Chang, Jin-Ding Huang
MPT0B292 was identified through screening of compounds able to selectively acetylate α-tubulins in cells and exhibited potent anti-tumor, anti-angiogenesis and anti-metastatic effects in vitro and in vivo. Because of poor water solubility, MPT0B292 is difficult to formulate with conventional approaches and hence difficulties are experienced in research practices. We mixed MPT0B292 with albumin in the aqueous solvent to form drug albumin nanoparticles with the size range around 333 nm. We investigated unbound fractions of these nanoparticles in different or same albumin concentration solutions...
February 1, 2017: Biopharmaceutics & Drug Disposition
https://www.readbyqxmd.com/read/28143710/hepatic-and-renal-mechanisms-underlying-the-osmopressor-response
#11
Tu H Mai, Emily M Garland, André Diedrich, David Robertson
Increased blood pressure (BP) is observed in patients with impaired baroreflexes after water drinking. The stimulus for this effect is low blood osmolality, and it has been termed the osmopressor response (OPR). The BP increase is associated with activation of the sympathetic nervous system and a requirement for transient receptor potential vanilloid 4 (TRPV4) channels. However, the mechanisms underlying the OPR are poorly understood. We tested the hypothesis that hypotonicity is sensed in the portal area to initiate the OPR...
January 27, 2017: Autonomic Neuroscience: Basic & Clinical
https://www.readbyqxmd.com/read/28132715/quantification-of-nonosmotic-sodium-storage-capacity-following-acute-hypertonic-saline-infusion-in-healthy-individuals
#12
Rik H G Olde Engberink, Nienke M G Rorije, Bert-Jan H van den Born, Liffert Vogt
The assumption that sodium accumulation in the human body is always accompanied by water retention has been challenged by data showing that sodium can be stored nonosmotically. Here we investigated the contribution of nonosmotic sodium storage to short-term sodium homeostasis after hypertonic saline infusion in healthy individuals on a low-sodium diet. During four hours after infusion, we compared the observed changes in plasma sodium concentration and urinary cation excretion with changes that were calculated with the Adrogue-Madias and Nguyen-Kurtz formula, formulations widely implemented to guide the treatment of dysnatremias...
January 26, 2017: Kidney International
https://www.readbyqxmd.com/read/28129470/the-water-channel-aqp1-is-expressed-in-human-atherosclerotic-vascular-lesions-and-aqp1-deficiency-augments-angiotensin-ii-induced-atherosclerosis-in-mice
#13
Pamela Wintmo, Søren H Johansen, Pernille B L Hansen, Jes S Lindholt, Sigitas Urbonavicius, Lars M Rasmussen, Peter Bie, Boye L Jensen, Jane Stubbe
AIM: The water channel aquaporin 1 (AQP1) promotes endothelial cell migration. It was hypothesized that AQP1 promotes neovascularization and growth of atherosclerotic plaques. METHODS: AQP1 immunoreactivity and protein abundance was examined in human and murine atherosclerotic lesions and aortic aneurysms. Apolipoprotein E (ApoE) knockout (-/-) and AQP1-/-ApoE-/- were developed and fed western diet (WD) for 8 and 16 weeks to accelerate the atherosclerosis process...
January 27, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28128932/high-thermoelectric-power-factor-organic-thin-films-through-combination-of-nanotube-multilayer-assembly-and-electrochemical-polymerization
#14
Mario Culebras, Chungyeon Cho, Michelle Krecker, Ryan Smith, Yixuan Song, Clara Gomez, Andres Cantarero, Jaime C Grunlan
In an effort to produce effective thermoelectric nanocomposites with multiwalled carbon nanotubes (MWCNT), layer-by-layer assembly was combined with electrochemical polymerization to create synergy that would produce a high power factor. Nanolayers of MWCNT stabilized with poly(diallyldimethylammonium chloride) or sodium deoxycholate were alternately deposited from water. Poly(3,4-ethylene dioxythiophene) [PEDOT] was then synthesized electrochemically by using this MWCNT-based multilayer thin film as the working electrode...
January 27, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28128418/p2x7-receptor-in-the-hippocampus-is-involved-in-gp120-induced-cognitive-dysfunction
#15
Y Liu, G Q Chen, B Y Liu, Q Chen, Y M Qian, S S Qin, C L Liu, C S Xu
To investigate the role of the P2X7 receptor in learning and memory dysfunction induced by HIV-1 envelope glycoprotein gp120 (gp120), we established HIV-1-associated dementia (HAD) animal models by intracerebroventricular (ICV) infusion of gp120 in rats. We observed gp120-induced cognitive dysfunction in the radial arm water maze test. Results showed that rats in the gp120 groups had longer escape latencies and more errors compared to those in the control group. For example, the average trial time in the 50-ng/day-gp120 group on day eight (16...
January 23, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28121417/adhesion-of-microdroplets-on-water-repellent-surfaces-toward-the-prevention-of-surface-fouling-and-pathogen-spreading-by-respiratory-droplets
#16
Jieke Jiang, Hengdi Zhang, Wenqing He, Tianzhong Li, Hualin Li, Peng Liu, Meijin Liu, Zhaoyue Wang, Zuankai Wang, Xi Yao
Biofouling caused by the adhesion of respiratory microdroplets generated in sneezing and coughing plays an important role in the spread of many infectious diseases. Although water-repellent surfaces are widely used for the long-term repellency of aqueous solutions, their repellency to pathogen-containing microdroplets is elusive. In this work, microdroplets from picoliter to nanoliter were successfully generated in a controlled manner to mimic the exhaled microdroplets in sneezing and coughing, which allowed us to evaluate the adhesion of microdroplets on both superhydrophobic and lubricant-infused "slippery" surfaces for the first time...
February 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28120305/safety-evaluation-of-fluoride-content-in-tea-infusions-consumed-in-the-azores-a-volcanic-region-with-water-springs-naturally-enriched-in-fluoride
#17
Diana Paula Silva Linhares, Patrícia Ventura Garcia, Leslie Amaral, Teresa Ferreira, Armindo Dos Santos Rodrigues
Tea is the second most commonly consumed beverage in the world. It is well recognized that the consumption of tea in high quantities can promote the development of fluorosis. The main objective of this study is to estimate the exposure to fluoride in the Azores through drinking tea prepared with water from different volcanic locations, by i) investigating the fluoride (F) content of various commercial brands of tea (Camellia sinensis) marketed in Azores and ii) comparing tea releasing rates of F according to brewing time, considering the fluoride concentration in the different types of water used for the infusion...
January 24, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28113139/lc-ms-ms-determination-of-a-human-mab-drug-candidate-in-rat-serum-using-an-isotopically-labeled-universal-mab-internal-standard
#18
Wenkui Li, Hui Lin, Yunlin Fu, Jimmy Flarakos
We report the application of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) bioanalytical method for the determination of a recombinant human immunoglobulin G1 (hIgG1), NVSMAb-1, in rat serum. A stable isotopically labeled universal monoclonal antibody (SILuMab), instead of stable isotopically labeled surrogate peptide, was employed as the internal standard. The internal standard was added to the sample matrix in the first step of the sample preparation process, which involved protein precipitation and pellet digestion...
January 4, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28111028/solute-and-water-transport-in-peritoneal-dialysis-a-case-based-primer
#19
Ramesh Khanna
Peritoneal dialysis (PD) is an effective therapy for patients with end-stage kidney disease. Dialysis solutions containing physiologic concentrations of electrolytes and base, as well as glucose often at supraphysiologic concentrations, are infused into the peritoneal cavity for solute and water exchange, and the patient's own peritoneal membrane is used for dialysis. The peritoneal membrane is dominated by small pores, which allow transport of water and small-molecular-size solutes, including electrolytes, by way of both diffusion and convection...
January 19, 2017: American Journal of Kidney Diseases: the Official Journal of the National Kidney Foundation
https://www.readbyqxmd.com/read/28109599/effect-of-intramammary-infusion-of-chitosan-hydrogels-at-drying-off-on-bovine-mammary-gland-involution
#20
S Lanctôt, P Fustier, A R Taherian, B Bisakowski, X Zhao, P Lacasse
The transition from lactation to the dry period in dairy cows is a period of high risk for acquiring new intramammary infections. This risk is reduced when the involution of the mammary gland is completed. Accordingly, approaches that speed up the involution process after drying-off could reduce the incidence of mastitis. The current study aimed to develop a biological response modifier that could be injected into cow teats to promote immune cell migration and speed up involution. Chitosan, a natural polysaccharide derived from chitin, is able to trigger host innate immunity...
January 18, 2017: Journal of Dairy Science
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