keyword
MENU ▼
Read by QxMD icon Read
search

Strep

keyword
https://www.readbyqxmd.com/read/27867675/a-case-of-thyroid-storm-associated-with-cardiomyopathy-and-poststreptococcal-glomerulonephritis
#1
Lisa J Underland, Gerson A Vallencia Villeda, Abhijeet Pal, Leslie Lam
Thyroid storm has a high mortality rate and is often associated with a precipitating factor such as intercurrent illness or infection. It is rare in pediatric patients. Cardiac disease in hyperthyroidism mostly manifests itself as tachycardia but more serious cardiac findings have also been described. A 5-year-old male with recent strep throat infection presented with dilated cardiomyopathy, hematuria, and symptoms and lab findings consistent with severe hyperthyroidism. He was diagnosed with thyroid storm secondary to concurrent Graves' disease and poststreptococcal glomerulonephritis (PSGN)...
2016: Case Reports in Pediatrics
https://www.readbyqxmd.com/read/27867058/application-of-strep-tactin-xt-for-affinity-purification-of-twin-strep-tagged-cb2-a-g-protein-coupled-cannabinoid-receptor
#2
Alexei Yeliseev, Lioudmila Zoubak, Thomas G M Schmidt
Human cannabinoid receptor CB2 belongs to the class A of G protein-coupled receptor (GPCR). High resolution structural studies of CB2 require milligram quantities of purified, structurally intact protein. Here we describe an efficient protocol for purification of this protein using the Twin-Strep-tag/Strep-Tactin XT system. To improve the affinity of interaction of the recombinant CB2 with the resin, the double repeat of the Strep-tag was attached either to the N- or C-terminus of CB2 via a short linker. The CB2 was isolated at high purity from dilute solutions containing high concentrations of detergents, glycerol and salts, by capturing onto the Strep-Tactin XT resin, and was eluted from the resin under mild conditions upon addition of biotin...
November 17, 2016: Protein Expression and Purification
https://www.readbyqxmd.com/read/27851403/1768-multifocal-necrotizing-fasciitis-due-to-group-a-strep-presenting-with-otalgia-in-a-pediatric-patient
#3
Kate Waldeck, Rodney Daniels, Michael Watson, Leena Shahbaz
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27818775/characterization-and-antimicrobial-susceptibility-of-one-antibiotic-sensitive-and-one-multidrug-resistant-corynebacterium-kroppenstedtii-strain-isolated-from-patients-with-granulomatous-mastitis
#4
I Fernández-Natal, D Rodríguez-Lázaro, T Marrodán-Ciordia, J A Sáez-Nieto, S Valdezate, H Rodríguez-Pollán, A Tauch, F Soriano
Human infections associated with Corynebacterium kroppenstedtii are rarely reported, and this organism is usually described as antibiotic sensitive. Almost all published cases of C. kroppenstedtii infections have been associated with breast pathology in women and have been described in New Zealand, France, Canada, India and Japan. Here we describe the microbiologic characteristics of two strains isolated from two women diagnosed of granulomatous mastitis in Spain. One C. kroppenstedtii isolate was antibiotic sensitive while the other was multidrug resistant...
November 2016: New Microbes and New Infections
https://www.readbyqxmd.com/read/27809838/characterization-of-plasmodium-falciparum-atp-dependent-dna-helicase-ruvb3
#5
Paviga Limudomporn, Saengduen Moonsom, Ubolsree Leartsakulpanich, Pattra Suntornthiticharoen, Songsak Petmitr, Michael Weinfeld, Porntip Chavalitshewinkoon-Petmitr
BACKGROUND: Malaria is one of the most serious and widespread parasitic diseases affecting humans. Because of the spread of resistance in both parasites and the mosquito vectors to anti-malarial drugs and insecticides, controlling the spread of malaria is becoming difficult. Thus, identifying new drug targets is urgently needed. Helicases play key roles in a wide range of cellular activities involving DNA and RNA transactions, making them attractive anti-malarial drug targets. METHODS: ATP-dependent DNA helicase gene (PfRuvB3) of Plasmodium falciparum strain K1, a chloroquine and pyrimethamine-resistant strain, was inserted into pQE-TriSystem His-Strep 2 vector, heterologously expressed and affinity purified...
November 3, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27789054/-management-of-preterm-labor
#6
G Kayem, E Lorthe, M Doret
OBJECTIVE: To define the management of preterm labor (MAP). METHOD: The literature search was conducted using computer databases Medline and the Cochrane Library for a period from 1969 to March 2016. RESULTS: Leukocytosis screening may be useful in case of hospitalization for Preterm labor (PTL). Its use is not routine (professional consensus). Screening for urinary tract infection by urine culture should be systematic and antibiotic treatment should be performed in cases of bacterial colonization or urinary tract infection for a period of 7 days (grade A)...
October 24, 2016: Journal de Gynécologie, Obstétrique et Biologie de la Reproduction
https://www.readbyqxmd.com/read/27781152/expression-and-characterization-of-thermotolerant-lipase-with-broad-ph-profiles-isolated-from-an-antarctic-pseudomonas-sp-strain-ams3
#7
Wahhida Latip, Raja Noor Zaliha Raja Abd Rahman, Adam Thean Chor Leow, Fairolniza Mohd Shariff, Mohd Shukuri Mohamad Ali
A gene encoding a thermotolerant lipase with broad pH was isolated from an Antarctic Pseudomonas strain AMS3. The recombinant lipase AMS3 was purified by single-step purification using affinity chromatography, yielding a purification fold of approximately 1.52 and a recovery of 50%. The molecular weight was approximately ∼60 kDa including the strep and affinity tags. Interestingly, the purified Antarctic AMS3 lipase exhibited broad temperature profile from 10-70 °C and stable over a broad pH range from 5...
2016: PeerJ
https://www.readbyqxmd.com/read/27770528/bacteriological-examination-of-the-abdominal-effusion-in-bacterial-peritonitis
#8
T Didbaridze, L Saginashvili, L Akhmeteli, B Iremashvili, N Gogokhia
This study provides a contemporary epidemiology of aspirates taken during surgery from the abdominal cavity among patients with bacterial peritonitis to identify the isolates and study their sensitivity to antibiotics. Our bacteriology investigations included isolation of poor cultures, and detection of microbes was conducted using a rapid identification system (API20E, API Staph, API Strep, API Ana, BioMerieux). Rapid tests for detection of oxidase and catalase activity were also used. Susceptibility of microorganisms to antibiotics was defined by the disc-diffusion method using standard discs (EUCAST guidelines 2015) according to Clinical Laboratory Standard Institute (CLSI) protocols (ATB strips: ATB G, ATB Staph, ATBANA, ATBPse, ATBStrep...
September 2016: Georgian Medical News
https://www.readbyqxmd.com/read/27720156/identification-of-factors-influencing-teat-dip-efficacy-trial-results-by-meta-analysis
#9
B D Enger, R R White, S C Nickerson, L K Fox
Two meta-analyses were conducted using data from peer-reviewed natural exposure (NE) and experimental challenge (EC) teat dip efficacy trials to identify factors influencing the new intramammary infection (IMI) rate. A NE data set containing 16 studies and an EC data set containing 21 studies were created. New IMI rate was calculated based on the percentage of new quarter infections per month (PNQI/mo) for each observation, in both data sets, and used as the dependent variable for model derivation. A linear, mixed-effects model with a random study effect, weighted by number of quarters eligible for infection, was derived for each data set...
October 5, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27695674/characteristics-of-mucoid-streptococcus-pyogenes-isolated-from-two-patients-with-pneumonia-in-a-local-community
#10
Nobuaki Mori, Sakiko Hosoo, Yoshitaka Oyamada, Yasushi Sera, Yasuhiro Yamada, Momoko Sugawara, Akiko Higuchi, Yasuko Aoki, Takashi Takahashi
Streptococcus pyogenes (Group A Streptococcus, GAS) infections can develop into life-threatening disorders. However, the occurrence of some GAS pneumonia cases is relatively rare in a local community. We report here characteristics of mucoid GAS isolates obtained from the sputum of two patients with pneumonia in a local community. Although case-patients did not have contact with each other, case-patient 1's child and case-patient 2's grandchild attended the same kindergarten where a GAS pharyngitis epidemic had occurred...
2016: IDCases
https://www.readbyqxmd.com/read/27668898/antimicrobial-susceptibility-against-penicillin-ampicillin-and-vancomycin-of-viridans-group-streptococcus-in-oral-microbiota-of-patients-at-risk-of-infective-endocarditis
#11
Serap Süzük, Banu Kaşkatepe, Mustafa Çetin
The viridans group Streptococci (VGS) are most abundant in the mouth; in some instances they might emerge as pathogens particularly in infective endocarditis (IE). In this study, we aimed to define and determine the susceptibility against antibiotics of VGS that are members of the oral microbiota of patients exhibiting a risk of developing IE. Forty-nine patients at risk of infective endocarditis were included in the study. Identification of the bacteria was performed using API STREP (bioMérieux, France). Gradient test strips (E-Test, France) were used to determine MIC of the bacteria against penicillin, ampicillin, and vancomycin...
September 1, 2016: Le Infezioni in Medicina
https://www.readbyqxmd.com/read/27642669/rapid-screening-for-group-b-streptococcus-in-near-term-pregnant-women-by-granada%C3%A2-biphasic-broth
#12
Mona Wassef, Doaa Ghaith, Rana M A Abdella, Mohamed Kamel
BACKGROUND: Prenatal screening for group B Streptococcus (GBS) colonization can reduce the incidence of neonatal GBS infections. We aimed to improve the screening-based approach of GBS in a limited resources antenatal care clinic by using Strep B Granada™ Biphasic Broth. METHODS: This study included 80 pregnant women between 35 and 37 weeks of gestation, who attended the antenatal care clinic of Kasr El-Aini University Hospital from November 2013 to January 2014...
October 3, 2016: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/27631785/enterococcus-species-in-the-oral-cavity-prevalence-virulence-factors-and-antimicrobial-susceptibility
#13
Edson Yukio Komiyama, Laura Soares Souto Lepesqueur, Cinthia Gomes Yassuda, Lakshman P Samaranayake, Nipuna B Parahitiyawa, Ivan Balducci, Cristiane Yumi Koga-Ito
Enterococci are considered as transient constituent components of the oral microbiome that may cause a variety of oral and systemic infections. As there is sparse data on the oral enterococcal prevalence, we evaluated the Enterococcus spp. and their virulence attributes including antimicrobial resistance in a healthy Brazilian cohort. A total of 240 individuals in different age groups were studied (children 4-11 yrs, adolescents 12-17 yrs, young adults 18-29 yrs, adults 30-59 yrs, elderly over 60 yrs). Oral rinses were collected and isolates were identified by API 20 Strep and confirmed by 16S rDNA sequencing...
2016: PloS One
https://www.readbyqxmd.com/read/27631026/biochemical-characterization-of-middle-east-respiratory-syndrome-coronavirus-helicase
#14
Adeyemi O Adedeji, Hilary Lazarus
Middle East respiratory syndrome coronavirus (MERS-CoV) helicase is a superfamily 1 helicase containing seven conserved motifs. We have cloned, expressed, and purified a Strep-fused recombinant MERS-CoV nonstructural protein 13 (M-nsp13) helicase. Characterization of its biochemical properties showed that it unwound DNA and RNA similarly to severe acute respiratory syndrome CoV nsp13 (S-nsp13) helicase. We showed that M-nsp13 unwound in a 5'-to-3' direction and efficiently unwound the partially duplex RNA substrates with a long loading strand relative to those of the RNA substrates with a short or no loading strand...
September 2016: MSphere
https://www.readbyqxmd.com/read/27537670/virtual-versus-face-to-face-clinical-simulation-in-relation-to-student-knowledge-anxiety-and-self-confidence-in-maternal-newborn-nursing-a-randomized-controlled-trial
#15
Shelley Cobbett, Erna Snelgrove-Clarke
BACKGROUND: Clinical simulations can provide students with realistic clinical learning environments to increase their knowledge, self-confidence, and decrease their anxiety prior to entering clinical practice settings. OBJECTIVE: To compare the effectiveness of two maternal newborn clinical simulation scenarios; virtual clinical simulation and face-to-face high fidelity manikin simulation. DESIGN: Randomized pretest-posttest design. SETTING: A public research university in Canada...
October 2016: Nurse Education Today
https://www.readbyqxmd.com/read/27493985/a-study-to-determine-if-addition-of-palatal-petechiae-to-centor-criteria-adds-more-significance-to-clinical-diagnosis-of-acute-strep-pharyngitis-in-children
#16
Kumara V Nibhanipudi
Objective. A study to determine if addition of palatal petechiae to Centor criteria adds more value for clinical diagnosis of acute strep pharyngitis in children. Hypothesis. In children, Centor Criteria does not cover all the symptoms and signs of acute strep pharyngitis. We hypothesize that addition of palatal petechiae to Centor Criteria will increase the possibility of clinical diagnosis of group A streptococcal pharyngitis in children. Methods. One hundred patients with a complaint of sore throat were enrolled in the study...
2016: Global Pediatric Health
https://www.readbyqxmd.com/read/27447448/sensitive-electrochemical-determination-of-mirnas-based-on-a-sandwich-assay-onto-magnetic-microcarriers-and-hybridization-chain-reaction-amplification
#17
R M Torrente-Rodríguez, S Campuzano, V Ruiz-Valdepeñas Montiel, J J Montoya, J M Pingarrón
A novel electrochemical approach for determination of miRNAs involving a sandwich hybridization assay onto streptavidin-magnetic beads (Strep-MBs), hybridization chain reaction (HCR) amplification and amperometric detection at disposable screen-printed carbon electrodes is reported. Using miRNA-21 as the target analyte, a dynamic linear range from 0.2 to 5.0nM with a 60pM (1.5fmol in 25μL) detection limit was obtained. The achieved sensitivity is 24-fold higher than a non-HCR amplification approach involving conventional sandwich type assay onto MBs...
December 15, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27386730/strep-throat
#18
(no author information available yet)
No abstract text is available yet for this article.
July 1, 2016: American Family Physician
https://www.readbyqxmd.com/read/27377190/influence-of-arginine-on-the-growth-arginine-metabolism-and-amino-acid-consumption-profiles-of-streptococcus-thermophilus-t1c2-in-controlled-ph-batch-fermentations
#19
S Huang, Z W Ai, X M Sun, G F Liu, S Zhai, M Zhang, H Chen, Z Feng
AIMS: The aim of this study was to elucidate the effect of arginine on the growth, arginine metabolism and amino acid consumption profiles of Streptococcus thermophilus T1C2. METHODS AND RESULTS: The growth kinetics, intracellular pH, extracellular osmotic pressure, expression of key genes in the arginine metabolism pathway and amino acid consumption profiles were analysed in chemically defined medium with different initial arginine concentrations. The results showed that arginine stimulated the growth of Strep...
September 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/27374000/rapid-antigen-detection-test-for-group-a-streptococcus-in-children-with-pharyngitis
#20
REVIEW
Jérémie F Cohen, Nathalie Bertille, Robert Cohen, Martin Chalumeau
BACKGROUND: Group A streptococcus (GAS) accounts for 20% to 40% of cases of pharyngitis in children; the remaining cases are caused by viruses. Compared with throat culture, rapid antigen detection tests (RADTs) offer diagnosis at the point of care (within five to 10 minutes). OBJECTIVES: To determine the diagnostic accuracy of RADTs for diagnosing GAS in children with pharyngitis. To assess the relative diagnostic accuracy of the two major types of RADTs (enzyme immunoassays (EIA) and optical immunoassays (OIA)) by indirect and direct comparison...
July 4, 2016: Cochrane Database of Systematic Reviews
keyword
keyword
92814
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"