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https://www.readbyqxmd.com/read/28811943/reviewing-the-emergence-of-lactococcus-garvieae-a-case-of-catheter-associated-urinary-tract-infection-caused-by-lactococcus-garvieae-and-escherichia-coli-coinfection
#1
Tatvam T Choksi, Farhan Dadani
Lactococcus garvieae is considered a low virulence organism which is rarely associated with human infections. Most of the reported cases have been associated with bacteremia with or without endocarditis. We report a rare case of catheter associated urinary tract infection (CAUTI) caused by Lactococcus garvieae and Escherichia coli coinfection without any bacteremia in a patient with indwelling urinary catheter placed for benign prostatic hyperplasia (BPH). The patient also had a history of gastroesophageal reflux disease (GERD) with long standing famotidine treatment...
2017: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/28761057/development-of-series-of-affinity-tags-in-streptomyces
#2
Xu-Ming Mao, Ning Sun, Yang Zheng, Yong-Quan Li
Streptomyces are of great biological and industrial significance due to their complex morphological development and ability to produce numerous secondary metabolites. However, the intrinsic biochemical mechanisms underlying morphogenesis and secondary metabolism are rarely revealed, partially because of the limited availability of the biochemical tools in Streptomyces. Here we provided series of integrative vectors with various affinity tags, including single tags 3×FLAG, 3×HA, 3×Strep-tag II, 18×His, 13×Myc, and dual tags, all of which were driven from a strong constitutive promoter ermEp*...
July 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28740504/2-4-thiazolidinedione-treatment-improves-the-innate-immune-response-in-dairy-goats-with-induced-subclinical-mastitis
#3
Fernanda Rosa, Johan S Osorio, Erminio Trevisi, Francisco Yanqui-Rivera, Charles T Estill, Massimo Bionaz
Mastitis is a major disease in dairy cows resulting in significant economic losses. In vitro works suggest that ruminants peroxisome proliferator-activated receptor gamma (PPARγ) can aid in improving the response to mastitis and can control milk fat synthesis. The objectives of the present experiment were to test if treatment with the putative PPARγ agonist 2,4-thiazolidinedione (TZD) improves (1) the response to subclinical mastitis and (2) milk fat production. Lactating goats received daily injections of 8 mg/kg BW of TZD or saline for 3 weeks...
2017: PPAR Research
https://www.readbyqxmd.com/read/28740426/efficacy-of-flurbiprofen-8-75-mg-delivered-as-a-spray-or-lozenge-in-patients-with-sore-throat-due-to-upper-respiratory-tract-infection-a-randomized-non-inferiority-trial-in-the-russian-federation
#4
Eugenia Radkova, Natalia Burova, Valeria Bychkova, Robert DeVito
OBJECTIVE: To assess the efficacy of flurbiprofen 8.75 mg delivered as a spray or lozenge in patients with sore throat due to upper respiratory tract infection (URTI). MATERIALS AND METHODS: This multicenter, double-blind, double-dummy, non-inferiority study randomized 440 adults with recent-onset, moderate-to-severe sore throat due to URTI to a single dose of either flurbiprofen 8.75 mg spray (n=218) or flurbiprofen 8.75 mg lozenge (n=222). The presence or absence of beta-hemolytic streptococci (A or C) was confirmed by culture tests (throat swab)...
2017: Journal of Pain Research
https://www.readbyqxmd.com/read/28710280/the-assembly-of-the-plant-urease-activation-complex-and-the-essential-role-of-the-urease-accessory-protein-g-ureg-in-delivery-of-nickel-to-urease
#5
Till Myrach, Anting Zhu, Claus-Peter Witte
Urease is a ubiquitous nickel metalloenzyme. In plants, its activation requires three urease accessory proteins (UAPs), UreD, UreF, and UreG. In bacteria, the UAPs interact with urease and facilitate activation, which involves the channeling of two nickel ions into the active site. So far this process has not been investigated in eukaryotes. Using affinity pull-downs of Strep-tagged UAPs from Arabidopsis and rice transiently expressed in planta, we demonstrate that a urease:UreD:UreF:UreG complex exists in plants and show its stepwise assembly...
July 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28632114/enterococcus-crotali-sp-nov-isolated-from-faecal-material-of-a-timber-rattlesnake
#6
Richard W McLaughlin, Patricia Lynn Shewmaker, Anne M Whitney, Ben W Humrighouse, Ana C Lauer, Vladimir N Loparev, Christopher A Gulvik, P A Cochran, Scot E Dowd
A facultatively anaerobic, Gram-stain-positive bacterium, designated ETRF1T, was found in faecal material of a timber rattlesnake (Crotalus horridus). Based on a comparative 16S rRNA gene sequence analysis, the isolate was assigned to the genus Enterococcus. The 16S rRNA gene sequence of strain ETRF1T showed >97 % similarity to that of the type strains of Enterococcus rotai, E. caccae, E. silesiacus, E haemoperoxidus, E. ureasiticus, E. moraviensis, E. plantarum, E. quebecensis, E. ureilyticus, E. termitis, E...
June 20, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28618168/streptococcus-pyogenes-quinolinate-salvage-pathway-structural-and-functional-studies-of-quinolinate-phosphoribosyl-transferase-and-nh3-dependent-nad-synthetase
#7
William T Booth, Trevor L Morris, David P Mysona, Milan J Shah, Linda K Taylor, Taylor W Karlin, Kathryn Clary, Karolina A Majorek, Lesa R Offermann, Maksymilian Chruszcz
Streptococcus pyogenes, also known as Group A Strep (GAS), is an obligate human pathogen that is responsible for millions of infections and numerous deaths per year. Infection manifestations can range from simple, acute pharyngitis to more complex, necrotizing fasciitis. To date, most treatments for GAS infections involve the use of common antibiotics including tetracycline and clindamycin. Unfortunately, new strains have been identified that are resistant to these drugs, therefore, new targets must be identified to treat drug-resistant strains...
August 2017: FEBS Journal
https://www.readbyqxmd.com/read/28602715/microstructure-dependent-binding-of-pigment-epithelium-derived-factor-pedf-to-type-i-collagen-fibrils
#8
Meagan Cauble, Phillip Yang, Ulrich Baumann, Jan M Gebauer, Bradford G Orr, Le T Duong, Mark M Banaszak Holl
Pigment epithelium derived factor (PEDF) is a multifunctional extracellular protein. In addition to its known anti-angiogenic and neurotrophic roles in collagen rich tissues, PEDF is thought to be involved in collagen fibril assembly due to its sequence specific binding to the collagen fibril and high expression in regions of active bone formation. In order to image the presence of the protein on the fibrils, PEDF was recombinantly made with a strep tag (strep-PEDF) and then gold nanoparticles conjugated to streptavidin (AuNP) were used as a secondary tag...
June 12, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/28569141/disease-burden-and-antimicrobial-resistance-of-invasive-group-b-streptococcus-among-infants-in-china-a-protocol-for-a-national-prospective-observational-study
#9
Wenjing Ji, Haiying Liu, Zhengjiang Jin, Aimin Wang, Xiaoping Mu, Xiaosong Qin, Weidong Wang, Chunyan Gao, Yuning Zhu, Xiaodan Feng, Jine Lei, Shangyang She, Lan Jiang, Jing Liu, Shuhua Yang, Zeshi Liu, Gang Li, Qiuhong Li, Dawen Guo, Muhammad Majid Aziz, Ali Hassan Gillani, Yu Fang
BACKGROUND: Group B Streptococcus (GBS) is a cause of neonatal sepsis, pneumonia, and meningitis that can lead to neurological sequelae in infants less than 3 months of age. The GBS disease burden is not known in China, therefore it cannot receive major attention. The main objectives of this study are the evaluation of the incidence of neonatal GBS infection, GBS case-fatality ratio, its serotypes and genotypes, bacterial resistance, clinical treatment and outcomes in China. METHODS: We are conducting a nation-wide, population-based, multi-center, prospective, observational cohort study in China from May 2016 to December 2017...
May 31, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28562551/final-diagnoses-and-probability-of-new-reason-for-encounter-at-an-urban-clinic-in-japan-a-4-year-observational-study
#10
Motoharu Fukushi, Yukishige Ishibashi, Naoki Nago
Past clinical data are not currently used to calculate pretest probabilities, as they have not been put into a database in clinical settings. This observational study was designed to determine the initial reasons for utilizing home visits or visits to an outpatient urban clinic in Japan.All family medical clinic outpatients and patients visited by the clinic (total = 11,688) over 1460 days were enrolled.We used a Bayes theorem-based clinical decision support system to analyze codes for initial reason-for-encounter (examination and final diagnosis: pretest probability) and final diagnosis of patients with fever (conditional pretest probability)...
June 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28554207/provider-type-and-management-of-common-visits-in-primary-care
#11
Douglas W Roblin, Hangsheng Liu, Lee F Cromwell, Michael Robbins, Brandi E Robinson, David Auerbach, Ateev Mehrotra
OBJECTIVES: Debate continues on whether nurse practitioners (NPs) and physician assistants (PAs) are more likely to order ancillary services, or order more costly services among alternatives, than primary care physicians (PCPs). We compared prescription medication and diagnostic service orders associated with NP/PA versus PCP visits for management of neck or back (N/B) pain or acute respiratory infection (ARI). STUDY DESIGN: Retrospective, observational study of visits from January 2006 through March 2008 in the adult primary care practice of Kaiser Permanente in Atlanta, Georgia...
April 2017: American Journal of Managed Care
https://www.readbyqxmd.com/read/28539650/the-effect-of-two-amino-acid-residue-substitutions-via-rna-editing-on-dark-operative-protochlorophyllide-oxidoreductase-in-the-black-pine-chloroplasts
#12
Haruki Yamamoto, Junko Kusumi, Hisanori Yamakawa, Yuichi Fujita
Dark-operative protochlorophyllide oxidoreductase (DPOR) is a key enzyme to produce chlorophyll in the dark. Among photosynthetic eukaryotes, all three subunits chlL, chlN, and chlB are encoded by plastid genomes. In some gymnosperms, two codons of chlB mRNA are changed by RNA editing to codons encoding evolutionarily conserved amino acid residues. However, the effect of these substitutions on DPOR activity remains unknown. We first prepared cyanobacterial ChlB variants with amino acid substitution(s) to mimic ChlB translated from pre-edited mRNA...
May 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28534309/environmental-monitoring-and-assessment-of-antibacterial-metabolite-producing-actinobacteria-screened-from-marine-sediments-in-south-coastal-regions-of-karnataka-india
#13
Sinosh Skariyachan, Shruthi Garka, Sushmitha Puttaswamy, Shobitha Shanbhogue, Raksha Devaraju, Rajeswari Narayanappa
Assessment of the therapeutic potential of secondary metabolite producing microorganisms from the marine coastal areas imparts scope and application in the field of environmental monitoring. The present study aims to screen metabolites with antibacterial potential from actionbacteria associated with marine sediments collected from south coastal regions of Karnataka, India. The actinobacteria were isolated and characterized from marine sediments by standard protocol. The metabolites were extracted, and antibacterial potential was analyzed against eight hospital associated bacteria...
June 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28530395/detection-of-prosthetic-joint-infection-based-on-magnetically-assisted-surface-enhanced-raman-spectroscopy
#14
Ariana Fargašová, Anna Balzerová, Robert Prucek, Miroslava Htoutou Sedláková, Kateřina Bogdanová, Jiří Gallo, Milan Kolář, Václav Ranc, Radek Zbořil
Accurate and rapid diagnosis of prosthetic joint infection (PJI) is vital for rational and effective therapeutic management of this condition. Several diagnostic strategies have been developed for discriminating between infected and noninfected cases. However, none of them can reliably diagnose the whole spectrum of clinical presentations of PJI. Here, we report a new method for PJI detection based on magnetically assisted surface enhanced Raman spectroscopy (MA-SERS) using streptavidin-modified magnetic nanoparticles (MNP@Strep) whose surface is functionalized with suitable biotinylated antibodies and then coated with silver nanoparticles by self-assembly...
June 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28529944/optimization-of-time-for-neural-stem-cells-transplantation-for-brain-stroke-in-rats
#15
Seyyed Mohyeddin Ziaee, Parisa Tabeshmehr, Khawaja Husnain Haider, Majidreza Farrokhi, Abdolhamid Shariat, Atena Amiri, Seyed Mojtaba Hosseini
BACKGROUND: Despite encouraging data in terms of neurological outcome, stem cell based therapy for ischemic stroke in experimental models and human patients is still hampered by multiple as yet un-optimized variables, i.e., time of intervention, that significantly influence the prognosis. The aim of the present study was to delineate the optimum time for neural stem cells (NSCs) transplantation after ischemic stroke. METHODS: The NSCs were isolated from 14 days embryo rat ganglion eminence and were cultured in NSA medium (neurobasal medium, 2% B27, 1% N2, bFGF 10 ng/mL, EGF 20 ng/mL and 1% pen/strep)...
2017: Stem Cell Investigation
https://www.readbyqxmd.com/read/28500845/hematopoietic-transcription-factors-and-differential-cofactor-binding-regulate-prkacb-isoform-expression
#16
Olga N Kuvardina, Stefanie Herkt, Annekarin Meyer, Lucas Schneider, Jasmin Yillah, Nicole Kohrs, Halvard Bonig, Erhard Seifried, Carsten Müller-Tidow, Jörn Lausen
Hematopoietic differentiation is controlled by key transcription factors, which regulate stem cell functions and differentiation. TAL1 is a central transcription factor for hematopoietic stem cell development in the embryo and for gene regulation during erythroid/megakaryocytic differentiation. Knowledge of the target genes controlled by a given transcription factor is important to understand its contribution to normal development and disease. To uncover direct target genes of TAL1 we used high affinity streptavidin/biotin-based chromatin precipitation (Strep-CP) followed by Strep-CP on ChIP analysis using ChIP promoter arrays...
April 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/28446575/evaluation-of-strepbselect-chromogenic-medium-and-the-fast-track-diagnostics-group-b-streptococcus-gbs-real-time-pcr-assay-compared-to-routine-culture-for-detection-of-gbs-during-antepartum-screening
#17
Deirdre L Church, Heather Baxter, Tracie Lloyd, Oscar Larios, Daniel B Gregson
Life-threatening infection in neonates due to group B Streptococcus (GBS) is preventable by screening of near-term pregnant women and treatment at delivery. A total of 295 vaginal-rectal swabs were collected from women attending antepartum clinics in Calgary, Alberta, Canada. GBS colonization was detected by the standard culture method (Strep B Carrot Broth subcultured to blood agar with a neomycin disk) and compared to recovery with Strep Group B Broth (Dalynn Biologicals) subcultured to StrepBSelect chromogenic medium (CM; Bio-Rad Laboratories) and the Fast-Track Diagnostics GBS real-time PCR (quantitative PCR [qPCR]) assay (Phoenix Airmid Biomedical Corp...
July 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28434736/incidence-of-clinical-mastitis-and-distribution-of-pathogens-on-large-chinese-dairy-farms
#18
Jian Gao, Herman W Barkema, Limei Zhang, Gang Liu, Zhaoju Deng, Lingjie Cai, Ruixue Shan, Shiyao Zhang, Jiaqi Zou, John P Kastelic, Bo Han
Knowledge of the incidence of clinical mastitis (CM) and the distribution of pathogens involved is essential for development of prevention and control programs as well as treatment protocols. No country-wide study on the incidence of CM and the distribution of pathogens involved has been conducted in China. Core objectives of this study were, therefore, to determine the cumulative incidence of CM and the distribution of pathogens causing CM on large Chinese (>500 cows) dairy farms. In addition, associations between the distribution of CM pathogens and bedding materials and seasonal factors were also investigated...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28430992/identifying-practice-related-factors-for-high-volume-prescribers-of-antibiotics-in-danish-general-practice
#19
Rune Aabenhus, Volkert Siersma, Håkon Sandholdt, Rasmus Køster-Rasmussen, Malene Plejdrup Hansen, Lars Bjerrum
Objectives: In Denmark, general practice is responsible for 75% of antibiotic prescribing in the primary care sector. We aimed to identify practice-related factors associated with high prescribers, including prescribers of critically important antibiotics as defined by WHO, after accounting for case mix by practice. Methods: We performed a nationwide register-based survey of antibiotic prescribing in Danish general practice from 2012 to 2013. The unit of analysis was the individual practice...
April 19, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28429165/the-use-of-rapid-test-quikread-go%C3%A2-strep-a-in-bacterial-pharyngotonsillitis-diagnosing-and-therapeutic-decisions
#20
E Stefaniuk, K Bosacka, M Wanke-Rytt, W Hryniewicz
Group A Streptococci (GAS) are the main causative agents of bacterial pharyngitis, which require antibiotic therapy. Rapid diagnostic tests detecting GAS combined with Centor/McIsaac score enable accurate differential diagnosis (viral vs. bacterial) and prompt commencement of targeted treatment. The aim of this study was to assess the specificity, sensitivity, PPV and NPV of QuikRead go® Strep A (Orion Diagnostica Oy, Finland) recommended for the detection of GAS in pharyngeal swabs. Quick diagnostic test results were compared with physical examination findings, Centor/McIsaac score and results of reference testing (conventional microbial cultures)...
April 21, 2017: European Journal of Clinical Microbiology & Infectious Diseases
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