keyword
MENU ▼
Read by QxMD icon Read
search

Strep

keyword
https://www.readbyqxmd.com/read/28446575/evaluation-of-strepb-select-chromagar-and-the-fast-track-diagnostics-group-b-streptococcus-gbs-real-time-polymerase-chain-reaction-assay-compared-to-routine-culture-for-detection-of-gbs-during-antepartum-screening
#1
Deirdre L Church, Heather Baxter, Tracie Lloyd, Oscar Larios, Daniel B Gregson
Life-threatening infection in neonates due to Group B Streptococcus (GBS) is preventable by screening near-term pregnant women and treatment at delivery. 295 vaginal-rectal swabs were collected from women attending antepartum clinics in Calgary. GBS colonization was detected by the standard culture method (Strep B Carrot broth subbed to blood agar with a neomycin disk), and compared to recovery using Strep Group B Broth (Dalynn Biologicals) subbed to StrepB Select chromagar (CA) (Bio-Rad Laboratories), and the Fast Track Diagnostics GBS real-time PCR (qPCR) assay (Phoenix Airmid Biomedical Corp...
April 26, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28434736/incidence-of-clinical-mastitis-and-distribution-of-pathogens-on-large-chinese-dairy-farms
#2
Jian Gao, Herman W Barkema, Limei Zhang, Gang Liu, Zhaoju Deng, Lingjie Cai, Ruixue Shan, Shiyao Zhang, Jiaqi Zou, John P Kastelic, Bo Han
Knowledge of the incidence of clinical mastitis (CM) and the distribution of pathogens involved is essential for development of prevention and control programs as well as treatment protocols. No country-wide study on the incidence of CM and the distribution of pathogens involved has been conducted in China. Core objectives of this study were, therefore, to determine the cumulative incidence of CM and the distribution of pathogens causing CM on large Chinese (>500 cows) dairy farms. In addition, associations between the distribution of CM pathogens and bedding materials and seasonal factors were also investigated...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28430992/identifying-practice-related-factors-for-high-volume-prescribers-of-antibiotics-in-danish-general-practice
#3
Rune Aabenhus, Volkert Siersma, Håkon Sandholdt, Rasmus Køster-Rasmussen, Malene Plejdrup Hansen, Lars Bjerrum
Objectives: In Denmark, general practice is responsible for 75% of antibiotic prescribing in the primary care sector. We aimed to identify practice-related factors associated with high prescribers, including prescribers of critically important antibiotics as defined by WHO, after accounting for case mix by practice. Methods: We performed a nationwide register-based survey of antibiotic prescribing in Danish general practice from 2012 to 2013. The unit of analysis was the individual practice...
April 19, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28429165/the-use-of-rapid-test-quikread-go%C3%A2-strep-a-in-bacterial-pharyngotonsillitis-diagnosing-and-therapeutic-decisions
#4
E Stefaniuk, K Bosacka, M Wanke-Rytt, W Hryniewicz
Group A Streptococci (GAS) are the main causative agents of bacterial pharyngitis, which require antibiotic therapy. Rapid diagnostic tests detecting GAS combined with Centor/McIsaac score enable accurate differential diagnosis (viral vs. bacterial) and prompt commencement of targeted treatment. The aim of this study was to assess the specificity, sensitivity, PPV and NPV of QuikRead go® Strep A (Orion Diagnostica Oy, Finland) recommended for the detection of GAS in pharyngeal swabs. Quick diagnostic test results were compared with physical examination findings, Centor/McIsaac score and results of reference testing (conventional microbial cultures)...
April 21, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28389190/detection-of-vancomycin-resistant-enterococci-vre-in-stool-specimens-submitted-for-clostridium-difficile-toxin-testing
#5
Sevim Özsoy, Arzu İlki
The aim of this study was to determine the association between Clostridium difficile (C. difficile) and vancomycin-resistant Enterococcus (VRE) and efficacy of screening stools submitted for C. difficile toxin assay for prevalence of VRE. Between April 2012 and February 2014, 158 stool samples submitted for C. difficile toxin to the Marmara University Microbiology Laboratory, were included in the study. Stool samples were analyzed by enzyme immuno assay test; VIDAS (bioMerieux, France) for Toxin A&B. Samples were inoculated on chromID VRE (bioMerieux, France) and incubated 24h at 37°C...
March 17, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28318563/characterization-of-enterococcus-species-isolated-from-marine-recreational-waters-by-maldi-tof-ms-and-rapid-id-api%C3%A2-20-strep-system
#6
Ana Paula Guarnieri Christ, Solange Rodrigues Ramos, Rodrigo Cayô, Ana Cristina Gales, Elayse Maria Hachich, Maria Inês Zanoli Sato
MALDI-TOF Mass Spectrometry Biotyping has proven to be a reliable method for identifying bacteria at the species level based on the analysis of the ribosomal proteins mass fingerprint. We evaluate the usefulness of this method to identify Enterococcus species isolated from marine recreational water at Brazilian beaches. A total of 127 Enterococcus spp. isolates were identified to species level by bioMérieux's API® 20 Strep and MALDI-TOF systems. The biochemical test identified 117/127 isolates (92%), whereas MALDI identified 100% of the isolates, with an agreement of 63% between the methods...
March 17, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28292304/design-and-implementation-of-electronic-health-record-integrated-clinical-prediction-rules-icpr-a-randomized-trial-in-diverse-primary-care-settings
#7
David A Feldstein, Rachel Hess, Thomas McGinn, Rebecca G Mishuris, Lauren McCullagh, Paul D Smith, Michael Flynn, Joseph Palmisano, Gheorghe Doros, Devin Mann
BACKGROUND: Clinical prediction rules (CPRs) represent a method of determining individual patient risk to help providers make more accurate decisions at the point of care. Well-validated CPRs are underutilized but may decrease antibiotic overuse for acute respiratory infections. The integrated clinical prediction rules (iCPR) study builds on a previous single clinic study to integrate two CPRs into the electronic health record and assess their impact on practice. This article discusses study design and implementation of a multicenter cluster randomized control trial of the iCPR clinical decision support system, including the tool adaptation, usability testing, staff training, and implementation study to disseminate iCPR at multiple clinical sites across two health care systems...
March 14, 2017: Implementation Science: IS
https://www.readbyqxmd.com/read/28288328/case-control-exploratory-study-of-cerebrospinal-fluid-chemokines-cytokines-and-lymphocyte-subsets-in-childhood-tourette-syndrome-with-positive-streptococcal-markers
#8
Michael R Pranzatelli, Elizabeth D Tate, Tyler J Allison
A longstanding question is whether neuroinflammation is present in children symptomatic for Tourette syndrome (TS) with positive streptococcal serology and throat cultures. The objective was to directly test for it using modern hypothesis-driven approaches. Profiling studies for 14 immune cell types (flow cytometry), 7 chemokines/cytokines (ELISA), oligoclonal bands, and other immunoglobulins were performed in this IRB-approved study of 5 children with TS and streptococcal markers compared to data from 26 non-inflammatory pediatric neurological controls...
March 10, 2017: Cytokine
https://www.readbyqxmd.com/read/28285457/group-a-streptococcus-acute-rheumatic-fever-and-rheumatic-heart-disease-epidemiology-and-clinical-considerations
#9
REVIEW
Liesl J Zühlke, Andrea Beaton, Mark E Engel, Christopher T Hugo-Hamman, Ganesan Karthikeyan, Judith M Katzenellenbogen, Ntobeko Ntusi, Anna P Ralph, Anita Saxena, Pierre R Smeesters, David Watkins, Peter Zilla, Jonathan Carapetis
Early recognition of group A streptococcal pharyngitis and appropriate management with benzathine penicillin using local clinical prediction rules together with validated rapi-strep testing when available should be incorporated in primary health care. A directed approach to the differential diagnosis of acute rheumatic fever now includes the concept of low-risk versus medium-to-high risk populations. Initiation of secondary prophylaxis and the establishment of early medium to long-term care plans is a key aspect of the management of ARF...
February 2017: Current Treatment Options in Cardiovascular Medicine
https://www.readbyqxmd.com/read/28283012/-evaluation-of-mascia-brunelli-rapid-antigen-test-in-the-diagnosis-of-group-a-streptococcal-pharyngitis
#10
Ayşe Barış, Nur Anlıaçık, Mehmet Emin Bulut, Rıdvan Deniz, Elif Yücel, Elif Aktaş
Pharyngitis in most cases is due to viral microorganisms however drug therapy without the detection of etiological agent leads to unnecessary use of antibiotics. On the other hand, when the etiologic agent is group A beta-hemolytic streptococci (GAS) it is important to identify the etiologic agent rapidly which will guide the treatment with appropriate antibiotics. The use of highly sensitive rapid tests will contribute significantly to early diagnosis and appropriate therapy. The aim of this study is to evaluate the efficacy of Mascia Brunelli rapid antigen test for the detection of GAS in throat swab samples...
January 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28283005/-comparison-of-maldi-tof-and-16s-rrna-methods-in-identification-of-viridans-group-streptococci
#11
Serap Süzük Yıldız, Banu Kaşkatepe, Salih Altınok, Mustafa Çetin, Alper Karagöz, Sümeyra Savaş
Accurate identification of viridans group streptococci (VGS) frequently encountered as a causative agent of infective endocarditis is always a challenge for the clinical microbiology laboratory. Clinical microbiology laboratories generally use semi automatic/full automatic systems, molecular methods and also conventional methods for the identification of these bacteria. There are recent published studies that have used MALDI-TOF (Matrix Assisted Laser Ionization Mass Spectrometry-Time of Flight) systems in the identification of VGS...
January 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28255712/purification-of-lat-containing-membranes-from-resting-and-activated-t-lymphocytes
#12
Claire Hivroz, Paola Larghi, Mabel Jouve, Laurence Ardouin
In T lymphocytes, the immune synapse is an active zone of vesicular traffic. Directional transport of vesicular receptors and signaling molecules from or to the immune synapse has been shown to play an important role in T-cell receptor (TCR) signal transduction. However, how vesicular trafficking is regulating the activation of T cells is still a burning question, and the characterization of these intracellular compartments remains the first step to understand this process. We describe herein a protocol, which combines a separation of membranes on flotation gradient with an affinity purification of Strep-tagged fusion transmembrane proteins with Strep-Tactin(®) resin, allowing the purification of membranes containing the Strep-tagged molecule of interest...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28237588/evaluating-a-commercial-pcr-assay-against-bacterial-culture-for-diagnosing-streptococcus-uberis-and-staphylococcus-aureus-throughout-lactation
#13
N M Steele, J H Williamson, R Thresher, R A Laven, J E Hillerton
The performance of a commercial, real-time PCR assay was compared with traditional bacterial culture for the identification of Streptococcus uberis and Staphylococcus aureus in bovine milk collected at different stages of lactation. Initial validation tests using fresh and frozen quarter milk samples identified factors that affected the success of the PCR. Therefore, the standard protocol was adjusted for samples collected at the first milking postpartum (colostrum) and from clinical mastitis cases. The adjustment involved PCR testing both undiluted and diluted (1 in 10 with sterile water) DNA extracts...
May 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28204577/strep-where-do-we-go-from-here
#14
Stanford T Shulman, Robert R Tanz
No abstract text is available yet for this article.
February 15, 2017: Journal of the Pediatric Infectious Diseases Society
https://www.readbyqxmd.com/read/28161160/non-culture-based-identification-of-mastitis-causing-bacteria-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#15
Juliana Regina Barreiro, Juliano Leonel Gonçalves, Patrícia Aparecida Campos Braga, Aline Gerato Dibbern, Marcos Nogueira Eberlin, Marcos Veiga Dos Santos
The purpose of this study was to evaluate the detection limit of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for direct identification, without previous microbiological culture, of bovine mastitis-causing bacteria from milk samples. Milk samples (n = 15) were experimentally contaminated with Staphylococcus aureus, Streptococcus uberis, Streptococcus agalactiae, Streptococcus dysgalactiae, and Escherichia coli to have bacterial counts ranging from 10(3) to 10(9) cfu/mL...
February 1, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28138890/adult-atopic-dermatitis-with-comorbid-atopic-disease-is-associated-with-increased-risk-of-infections-a-population-based-cross-sectional-study
#16
Saisindhu Narala, Tissa R Hata
INTRODUCTION: Atopic dermatitis (AD) is related to other atopic diseases asthma and allergic rhinitis. It is known that those with asthma or allergic rhinitis have impaired immune responses that may predispose them to infections. This study sought to determine whether adult AD is associated with systemic infections, and whether association is strengthened in those with AD plus another atopic disease. METHODS: This cross-sectional study obtained information from adults in the 2010 and the 2012 National Health Interview Survey (NHIS)...
March 2017: Dermatology and Therapy
https://www.readbyqxmd.com/read/28135881/streptococcal-pharyngitis-and-appendicitis-in-children
#17
Jason W Nielsen, Stuart A Abel, Brian Kenney
BACKGROUND: Several pathologies, including pharyngitis, are associated with abdominal pain that can mimic appendicitis. We sought to further understand the link between appendicitis-like symptoms and streptococcal (strep) pharyngitis. METHODS: All patients undergoing ultrasound imaging for appendicitis in our emergency department during 2013 were reviewed (n = 1572). A total of 207 patients were identified who underwent both ultrasound for appendicitis and testing for strep pharyngitis...
January 1, 2017: Clinical Pediatrics
https://www.readbyqxmd.com/read/28131414/diversity-of-enterococcus-faecalis-genotypes-from-multiple-oral-sites-associated-with-endodontic-failure-using-repetitive-sequence-based-polymerase-chain-reaction-and-arbitrarily-primed-polymerase-chain-reaction
#18
Maraísa G Delboni, Brenda P F A Gomes, Priscila A Francisco, Fabrício B Teixeira, David Drake
INTRODUCTION: The aim of this study was to evaluate the diversity and similarity of Enterococcus faecalis genotype isolates from multiple oral sites using repetitive sequence-based polymerase chain reaction and arbitrarily primed polymerase chain reaction (AP-PCR). METHODS: Forty-two endodontically treated teeth with apical periodontitis were selected. A total of 126 microbial samples were collected from 3 different sites (saliva, pulp chamber, and root canals, all n = 42) during the nonsurgical retreatment procedures...
March 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28027278/group-b-streptococcal-colonization-among-pregnant-women-in-delhi-india
#19
Manu Chaudhary, Marcia A Rench, Carol J Baker, Pushpa Singh, Charoo Hans, Morven S Edwards
BACKGROUND: Little is known regarding maternal group B streptococcal (GBS) colonization prevalence and capsular (CPS) serotype distribution among pregnant women in India. The objective of this prospective cohort study was to determine GBS recto-vaginal colonization prevalence in pregnant women at Dr. Ram Manohar Lohia Hospital in Delhi, India. METHODS: Literature review identified reports from India assessing GBS colonization prevalence in pregnant women. Rectal and vaginal swabs were inoculated into Strep B Carrot Broth™ and subcultured onto GBS Detect™ plates...
December 23, 2016: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28006981/accurate-detection-of-streptococcus-pyogenes-at-the-point-of-care-using-the-cobas-liat-strep-a-nucleic-acid-test
#20
Fangnian Wang, Yu Tian, Lingjun Chen, Robert Luo, Joanna Sickler, Oliver Liesenfeld, Shuqi Chen
The performance of a polymerase chain reaction-based point-of-care assay, the cobas Strep A Nucleic Acid Test for use on the cobas Liat System (cobas Liat Strep A assay), for the detection of group A Streptococcus bacteria was evaluated in primary care settings. Throat swab specimens from 427 patients were tested with the cobas Liat Strep A assay and a rapid antigen detection test (RADT) by existing medical staff at 5 primary care clinics, and results were compared with bacterial culture. The cobas Liat Strep A assay demonstrated equivalent sensitivity (97...
December 1, 2016: Clinical Pediatrics
keyword
keyword
92814
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"