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https://www.readbyqxmd.com/read/29331467/canadian-national-dairy-study-herd-level-milk-quality
#1
C A Bauman, H W Barkema, J Dubuc, G P Keefe, D F Kelton
The objective of this study was to estimate Canadian national milk quality parameters and estimate the bulk tank milk (BTM) prevalence of 4 mastitis pathogens, Staphylococcus aureus, Streptococcus agalactiae, Mycoplasma bovis, and Prototheca spp., on Canadian dairy farms. A questionnaire was sent to all Canadian dairy producers. Of the 1,062 producers who completed the questionnaire, 374 producers from across the country were visited and milking hygiene was assessed. Farm-level milk quality data for all Canadian dairy producers was collected from the provincial marketing boards and combined with the questionnaire and farm visit data...
January 10, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29305958/description-of-a-novel-mutation-in-the-atpc-gene-in-optochin-resistant-streptococcus-pneumoniae-strains-isolates-from-tunisia
#2
Anis Raddaoui, Farah Ben Tanfous, Wafa Achour, Rekaya Baaboura, Assia Benhassen
The identification of Streptocooccus pneumoniae among other α-hemolytic streptococci is based on phenotypic or genotypic characteristics as colony morphology, bile solubility and optochin susceptibility. We report in this study three optochin-resistant S. pneumoniae isolated from immunocompromised patients in Tunisia. The three isolates were positive for bile solubility test. The biochemical identification with API® 20 Strep was not discriminatory for two strains. The three strains had different serotypes (6C, 19F and 23F) and three different sequence types (ST386, ST320, and ST326)...
January 3, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29305544/evaluation-of-simplexa%C3%A2-group-a-strep-direct-kit-compared-to-hologic%C3%A2-group-a-streptococcal-direct-assay-for-detection-of-group-a-streptococcus-gas-in-throat-swabs
#3
Deirdre L Church, Tracie Lloyd, Oscar Larios, Daniel B Gregson
Diagnosis of bacterial pharyngitis is confirmed by detection of Group A Streptococcus (GAS) in patient throat samples. Testing of throat samples has historically relied on culture but new molecular methods allow much faster test turnaround time (i.e., same day vs. 48-72h for culture). Our laboratory uses the Hologic® GAS Direct (GASD) assay for screening more than 125,000 throat samples per annum. Simplexa™ GAS Direct is a new real-time PCR (qPCR) assay that does not require initial DNA extraction. Performance of Simplexa qPCR was compared to GASD...
January 5, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29305539/comparison-of-the-alere-i-and-bd-veritor-in-the-detection-of-group-a-streptococcus-and-the-hypothetical-impact-of-results-on-antibiotic-utilization
#4
Gregory J Berry, Catherine R Miller, Mariana Moreno Prats, Christopher Marquez, Olajumoke O Oladipo, Michael J Loeffelholz, John R Petersen
Rapid detection of Group A Streptococcus (GAS) is an integral component of treatment decisions in the clinic, especially in the pediatric population. We prospectively collected 216 specimens from symptomatic, predominantly pediatric patients and evaluated the performance of the Alere™ i Strep A test (Alere i) (Alere Inc., Scarborough, ME) and the BD Veritor™ System (BD Veritor) (Becton, Dickinson and Company, Sparks, MD), with culture results as a comparator. Real time (RT)-PCR was performed as an arbiter in discordant cases...
January 5, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29285648/bacteria-from-bronchoalveolar-lavage-fluid-from-children-with-suspected-chronic-lower-respiratory-tract-infection-results-from-a-multi-center-cross-sectional-study-in-spain
#5
Amparo Escribano Montaner, Juan García de Lomas, José Ramón Villa Asensi, Oscar Asensio de la Cruz, Olga de la Serna Blázquez, Mikel Santiago Burruchaga, Pedro Mondéjar López, Alba Torrent Vernetta, Yang Feng, Melissa K Van Dyke, Janet Reyes, Pilar Garcia-Corbeira, Carla A Talarico
This cross-sectional study assessed the prevalence of bacteria isolated from Spanish children with suspected chronic lower respiratory tract infection (LRTI) for whom bronchoalveolar lavage (BAL) was indicated. BAL fluid (BALF) was collected from 191 children (aged ≥ 6 months to < 6 years, with persistent or recurrent respiratory symptoms, non-responders to usual treatment) and cultured. Nasopharyngeal swabs (NPSs) were also obtained and cultured to assess concordance of BALF and NPS findings in the same patient...
December 29, 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/29281013/characterization-of-a-novel-miia-domain-containing-protein-mdce-in-bradyrhizobium-spp
#6
David Durán, Juan Imperial, José Palacios, Tomás Ruiz-Argüeso, Michael Göttfert, Susanne Zehner, Luis Rey
Several genes coding for proteins with metal ion-inducible autocleavage (MIIA) domains were identified in type III secretion system tts gene clusters from draft genomes of recently isolated Bradyrhizobium spp. MIIA domains have been first described in the effectors NopE1 and NopE2 of Bradyrhizobium diazoefficiens USDA 110. All identified genes are preceded by tts box promoter motifs. The identified proteins contain one or two MIIA domains. A phylogenetic analysis of 35 MIIA domain sequences from 16 Bradyrhizobium strains revealed four groups...
December 21, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29278689/trail-induces-apoptosis-but-not-necroptosis-in-colorectal-and-pancreatic-cancer-cells-preferentially-via-the-trail-r2-dr5-receptor
#7
Zuzana Nahacka, Jan Svadlenka, Martin Peterka, Marie Ksandrova, Simona Benesova, Jiri Neuzil, Ladislav Andera
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a cytokine that can trigger apoptosis in many types of human cancer cells via engagement of its two pro-apoptotic receptors TRAIL-R1 (DR4) and TRAIL-R2 (DR5). TRAIL can also activate several other signaling pathways such as activation of stress kinases, canonical NF-κB signaling and necroptosis. Though both receptors are ubiquitously expressed, their relative participation in TRAIL-induced signaling is still largely unknown. To analyze TRAIL receptor-specific signaling, we prepared Strep-tagged, trimerized variants of recombinant human TRAIL with high affinity for either DR4 or DR5 receptor...
December 23, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29275942/short-communication-lactose-enhances-bile-tolerance-of-yogurt-culture-bacteria
#8
Behannis Mena, Kayanush Aryana
Lactose is an energy source for culture bacteria. Bile tolerance is an important probiotic property. Our aim was to elucidate the effect of lactose on bile tolerance of yogurt starter culture Lactobacillus bulgaricus LB-12 and Streptococcus thermophilus ST-M5. Bile tolerance of pure cultures was determined using 0.3% oxgall in MRS THIO broth (Difco, Becton Dickinson, Sparks, MD) for L. bulgaricus and 0.3% oxgall in M17 broth (Oxoid, Basingstoke, UK) for Strep. thermophilus. Lactose was added to both broths at 0 (control), 1, 3, and 5% (wt/vol) broth...
December 21, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29238480/maternal-recto-vaginal-colonization-in-term-and-preterm-deliveries
#9
Mostafa Javanian, Zahra Akbarian Rad, Mohsen Haghshenas Mojaveri, Azita Ghanbarpour Shiadeh, Soheil Ebrahimpour
Background and aim: Maternal recto-vaginal organisms are the main cause of early-onset sepsis in neonates. The aim of this study was to determine the most common organisms and compare maternal recto-vaginal colonization in term and preterm delivery. Methods: This cross-sectional study was done from October 2014 through October 2016 among participants referred to Ayatollah Rouhani Hospital in Babol, Northern Iran. All pregnant women within gestational age (GA) ≥ 26 weeks and labor pain were included in this study...
October 2017: Electronic Physician
https://www.readbyqxmd.com/read/29185677/calculated-decisions-centor-score-modified-mcisaac-for-strep-pharyngitis
#10
Graham Walker, Rachel Kwon
No abstract text is available yet for this article.
December 1, 2017: Pediatric Emergency Medicine Practice
https://www.readbyqxmd.com/read/29183876/canadian-mother-whose-son-died-from-strep-throat-sentenced-to-three-years-in-prison
#11
Owen Dyer
No abstract text is available yet for this article.
November 28, 2017: BMJ: British Medical Journal
https://www.readbyqxmd.com/read/29171063/enzyme-linked-immunosorbent-assays-elisa-based-on-thread-paper-and-fabric
#12
Ariana Gonzalez, Michelle Gaines, Laura Y Gallegos, Ricardo Guevara, Frank A Gomez
This paper describes enzyme-linked immunosorbent assays (ELISAs) utilizing microfluidic thread/paper-based analytical devices (μTPAD), microfluidic fabric-based analytical devices (μFAD), and microfluidic thread-based analytical devices (μTAD). Here, the quantitative detection of biotinylated goat anti-mouse IgG (system one) and rabbit IgG (system two) antibodies via colorimetric analysis is detailed. In both systems, antibody is spotted on the detection site and subjected to a series of washes, addition of streptavidin-alkaline phosphatase (Strep-ALP) (system 1) or alkaline phosphatase (ALP)-conjugated secondary antibody (system 2), and colorimetric substrate...
November 24, 2017: Electrophoresis
https://www.readbyqxmd.com/read/29147115/effects-of-aminoglycoside-antibiotics-on-human-embryonic-stem-cell-viability-during-differentiation-in-vitro
#13
Divya S Varghese, Shama Parween, Mustafa T Ardah, Bright Starling Emerald, Suraiya A Ansari
Human embryonic stem cells (hESCs) are being used extensively in array of studies to understand different mechanisms such as early human embryogenesis, drug toxicity testing, disease modeling, and cell replacement therapy. The protocols for the directed differentiation of hESCs towards specific cell types often require long-term cell cultures. To avoid bacterial contamination, these protocols include addition of antibiotics such as pen-strep and gentamicin. Although aminoglycosides, streptomycin, and gentamicin have been shown to cause cytotoxicity in various animal models, the effect of these antibiotics on hESCs is not clear...
2017: Stem Cells International
https://www.readbyqxmd.com/read/29143175/vectors-for-expression-of-signal-peptide-dependent-proteins-in-baculovirus-insect-cell-systems-and-their-application-to-expression-and-purification-of-the-high-affinity-immunoglobulin-gamma-fc-receptor-i-in-complex-with-its-gamma-chain
#14
Le T M Le, Jens R Nyengaard, Monika M Golas, Bjoern Sander
Integral membrane proteins play a central role in various cellular functions and are important therapeutic targets. However, technical challenges in the overexpression and purification of membrane proteins often represent a limiting factor for biochemical and structural studies. Here, we constructed a set of vectors, derivatives of MultiBac vectors that can be used to express proteins with a cleavable N-terminal signal peptide in insect cells. We propose these vectors for expression of type I membrane proteins and other secretory pathway proteins that require the signal recognition particle for translocation to the endoplasmic reticulum (ER)...
November 15, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/29120349/magnetic-beads-based-sensor-with-tailored-sensitivity-for-rapid-and-single-step-amperometric-determination-of-mirnas
#15
Eva Vargas, Rebeca M Torrente-Rodríguez, Víctor Ruiz-Valdepeñas Montiel, Eloy Povedano, María Pedrero, Juan J Montoya, Susana Campuzano, José M Pingarrón
This work describes a sensitive amperometric magneto-biosensor for single-step and rapid determination of microRNAs (miRNAs). The developed strategy involves the use of direct hybridization of the target miRNA (miRNA-21) with a specific biotinylated DNA probe immobilized on streptavidin-modified magnetic beads (MBs), and labeling of the resulting heteroduplexes with a specific DNA-RNA antibody and the bacterial protein A (ProtA) conjugated with an horseradish peroxidase (HRP) homopolymer (Poly-HRP40) as an enzymatic label for signal amplification...
November 9, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29112476/cross-sectional-evaluation-of-plasma-vitamin-d-levels-in-a-large-cohort-of-italian-patients-with-pediatric-autoimmune-neuropsychiatric-disorders-associated-with-streptococcal-infections
#16
Stefano Stagi, Gemma Lepri, Donato Rigante, Marco Matucci Cerinic, Fernanda Falcini
BACKGROUND: Pediatric autoimmune neuropsychiatric disorders associated with streptococcal infections (PANDAS) are immune-mediated diseases characterized by obsessive-compulsive symptoms and/or tics triggered by group A Streptococcus infections. Despite the well-known action of 25-hydroxyvitamin D [25(OH)D] on different conditions driven by systemic inflammation, there are no data about the 25(OH)D status in patients with PANDAS. AIMS: To evaluate plasma 25(OH)D levels in a large cohort of children and adolescents with PANDAS and comparing the results with healthy controls...
November 7, 2017: Journal of Child and Adolescent Psychopharmacology
https://www.readbyqxmd.com/read/29099353/streptococcus-bovimastitidis-sp-nov-isolated-from-a-dairy-cow-with-mastitis
#17
Stefan P W de Vries, Nazreen F Hadjirin, Elizabeth M Lay, Ruth N Zadoks, Sharon J Peacock, Julian Parkhill, Andrew J Grant, Scott McDougall, Mark A Holmes
Here we describe a new species of the genus Streptococcus that was isolated from a dairy cow with mastitis in New Zealand. Strain NZ1587(T) was Gram-positive, coccus-shaped and arranged as chains, catalase and coagulase negative, γ-haemolytic and negative for Lancefield carbohydrates (A-D, F and G). The 16S rRNA sequence did not match sequences in the NCBI 16S rRNA or GreenGenes databases. Taxonomic classification of strain NZ1587(T) was investigated using 16S rRNA and core genome phylogeny, genome-wide average nucleotide identity (ANI) and predicted DNA-DNA hybridisation (DDH) analyses...
November 3, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29091937/phenome-wide-association-study-using-research-participants-self-reported-data-provides-insight-into-the-th17-and-il-17-pathway
#18
Margaret G Ehm, Jennifer L Aponte, Mathias N Chiano, Laura M Yerges-Armstrong, Toby Johnson, Jonathan N Barker, Suzanne F Cook, Akanksha Gupta, David A Hinds, Li Li, Matthew R Nelson, Michael A Simpson, Chao Tian, Linda C McCarthy, Deepak K Rajpal, Dawn M Waterworth
A phenome-wide association study of variants in genes in the Th17 and IL-17 pathway was performed using self-reported phenotypes and genetic data from 521,000 research participants of 23andMe. Results replicated known associations with similar effect sizes for autoimmune traits illustrating self-reported traits can be a surrogate for clinically assessed conditions. Novel associations controlling for a false discovery rate of 5% included the association of the variant encoding p.Ile684Ser in TYK2 with increased risk of tonsillectomy, strep throat occurrences and teen acne, the variant encoding p...
2017: PloS One
https://www.readbyqxmd.com/read/29069738/hematopoietic-transcription-factors-and-differential-cofactor-binding-regulate-prkacb-isoform-expression
#19
Olga N Kuvardina, Stefanie Herkt, Annekarin Meyer, Lucas Schneider, Jasmin Yillah, Nicole Kohrs, Halvard Bonig, Erhard Seifried, Carsten Müller-Tidow, Jörn Lausen
Hematopoietic differentiation is controlled by key transcription factors, which regulate stem cell functions and differentiation. TAL1 is a central transcription factor for hematopoietic stem cell development in the embryo and for gene regulation during erythroid/megakaryocytic differentiation. Knowledge of the target genes controlled by a given transcription factor is important to understand its contribution to normal development and disease. To uncover direct target genes of TAL1 we used high affinity streptavidin/biotin-based chromatin precipitation (Strep-CP) followed by Strep-CP on ChIP analysis using ChIP promoter arrays...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/29045629/signs-and-symptoms-of-group-a-versus-non-group-a-strep-throat-a-meta-analysis
#20
Thuy N Thai, Ariella P Dale, Mark H Ebell
Introduction: Both non-Group A streptococcal (non-GAS) pharyngitis and Group A streptococcal (GAS) pharyngitis are commonly found in patients with sore throat. It is not known whether or not they present with similar signs and symptoms compared to patients with non-streptococcal pharyngitis. Methods: MEDLINE was searched for prospective studies that reported throat culture for both GAS and non-GAS as a reference standard, and reported at least one sign, symptom, or the Centor score...
October 13, 2017: Family Practice
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