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Human Factors Engineering Study

Gretchen M Thomsen, Pablo Avalos, Annie A Ma, Mor Alkaslasi, Noell Cho, Livia Wyss, Jean-Philippe Vit, Marlesa Godoy, Patrick Suezaki, Oksana Shelest, Krystof S Bankiewicz, Clive N Svendsen
Early dysfunction of cortical motor neurons may underlie the initiation of amyotrophic lateral sclerosis (ALS). As such, the cortex represents a critical area of ALS research and a promising therapeutic target. In the current study, human cortical-derived neural progenitor cells engineered to secrete glial cell line-derived neurotrophic factor (GDNF) were transplanted into the SOD1G93A ALS rat cortex, where they migrated, matured into astrocytes, and released GDNF. This protected motor neurons, delayed disease pathology and extended survival of the animals...
April 15, 2018: Stem Cells
Qingqing Yao, Yangxi Liu, Balaranjan Selvaratnam, Ranjit T Koodali, Hongli Sun
Controlled delivery systems play a critical role in the success of bone morphogenetic proteins (i.e., BMP2 and BMP7) for challenged bone repair. Instead of single-drug release that is currently and commonly prevalent, dual-drug delivery strategies are highly desired to achieve effective bone regeneration because natural bone repair process is driven by multiple factors. Particularly, angiogenesis is essential for osteogenesis and requires more than just one factor (e.g., Vascular Endothelial Growth Factor, VEGF)...
April 9, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
Ying Lu, Zhuojun Wang, Lu Chen, Jia Wang, Shulin Li, Caixia Liu, Dong Sun
Amniotic fluid is an alternative source of stem cells, and human amniotic fluid-derived stem cells (AFSCs) obtained from a small amount of amniotic fluid collected during the second trimester represent a novel source for use in regenerative medicine. These AFSCs are characterized by lower diversity, a higher proliferation rate and a wider differentiation capability than adult mesenchymal stem cells (MSCs). AFSCs are selected based on the cell surface marker c-kit receptor (CD117) using immunomagnetic sorting...
March 15, 2018: Stem Cells and Development
Kazim Sahin, Engin Yenice, Mehmet Tuzcu, Cemal Orhan, Cengizhan Mizrak, Ibrahim H Ozercan, Nurhan Sahin, Bahiddin Yilmaz, Birdal Bilir, Bulent Ozpolat, Omer Kucuk
Background: Dietary intake of lycopene has been associated with a reduced risk of ovarian cancer, suggesting its chemopreventive potential against ovarian carcinogenesis. Lycopene's molecular mechanisms of action in ovarian cancer have not been fully understood. Therefore, in the present study, we investigated the effects of lycopene on the ovarian cancer formation using the laying hen model, a biologically relevant animal model of spontaneous ovarian carcinogenesis due to high incidence rates similar to humans...
March 2018: Journal of Cancer Prevention
Yoke Chin Chai, Luis Freitas Mendes, Nick van Gastel, Geert Carmeliet, Frank P Luyten
Rapid neovascularization of a tissue-engineered (TE) construct by the host vasculature is quintessential to warrant effective bone regeneration. This process can be promoted through active induction of angiogenic growth factor secretion or by implementation of in vitro pre-vascularization strategies. In this study, we aimed at optimizing the pro-angiogenic effect of Cobalt (Co2+ ) to enhance vascular endothelial growth factor (VEGF) expression by human periosteum-derived mesenchymal stem cells (hPDCs). Simultaneously we set out to promote microvascular network formation by co-culturing with human umbilical vein endothelial cells (HUVECs)...
April 4, 2018: Acta Biomaterialia
Célia M Manaia, Jaqueline Rocha, Nazareno Scaccia, Roberto Marano, Elena Radu, Francesco Biancullo, Francisco Cerqueira, Gianuário Fortunato, Iakovos C Iakovides, Ian Zammit, Ioannis Kampouris, Ivone Vaz-Moreira, Olga C Nunes
Wastewater is among the most important reservoirs of antibiotic resistance in urban environments. The abundance of carbon sources and other nutrients, a variety of possible electron acceptors such as oxygen or nitrate, the presence of particles onto which bacteria can adsorb, or a fairly stable pH and temperature are examples of conditions favouring the remarkable diversity of microorganisms in this peculiar habitat. The wastewater microbiome brings together bacteria of environmental, human and animal origins, many harbouring antibiotic resistance genes (ARGs)...
April 4, 2018: Environment International
Ee-Seul Kang, Da-Seul Kim, Intan Rosalina Suhito, Wanhee Lee, Inbeom Song, Tae-Hyung Kim
Background: In the past decade, stem cells, with their ability to differentiate into various types of cells, have been proven to be resourceful in regenerative medicine and tissue engineering. Despite the ability to repair damaged parts of organs and tissues, the use of stem cells still entails several limitations, such as low differentiation efficiency and difficulties in guiding differentiation. To address these limitations, nanotechnology approaches have been recently implemented in stem cell research...
2018: Biomaterials Research
Xinyu Qiu, Shiyu Liu, Hao Zhang, Bin Zhu, Yuting Su, Chenxi Zheng, Rong Tian, Miao Wang, Huijuan Kuang, Xinyi Zhao, Yan Jin
BACKGROUND: Skeletal muscle plays an important role in the body's physiology but there are still no effective treatments for volumetric muscle loss (VML) resulting from severe traumatic injury or tumor excision. Recent studies show that a tissue engineering strategy using a compound containing mesenchymal stem cells (MSCs) and decellularized extracellular matrix (ECM) scaffold generates significant regenerative effects on VML injury, but the underlying mechanisms are not fully understood...
April 3, 2018: Stem Cell Research & Therapy
Byoung Soo Kim, Yang Woo Kwon, Jeong-Sik Kong, Gyu Tae Park, Ge Gao, Wonil Han, Moon-Bum Kim, Hyungseok Lee, Jae Ho Kim, Dong-Woo Cho
3D cell-printing technique has been under spotlight as an appealing biofabrication platform due to its ability to precisely pattern living cells in pre-defined spatial locations. In skin tissue engineering, a major remaining challenge is to seek for a suitable source of bioink capable of supporting and stimulating printed cells for tissue development. However, current bioinks for skin printing rely on homogeneous biomaterials, which has several shortcomings such as insufficient mechanical properties and recapitulation of microenvironment...
March 23, 2018: Biomaterials
Mahshid Sadat Kashef-Saberi, Nasim Hayati Roodbari, Kazem Parivar, Saeid Vakilian, Hana Hanee-Ahvaz
Over the last few decades, great advancements have been achieved in the field of bone tissue engineering (BTE). Containing a great number of growth factors needed in the process of osteogenesis, platelet rich plasma (PRP) has gained a great deal of attention. However, due to the contradictory results achieved in different studies, its effectiveness remains a mystery. Therefore, in this study, we investigated in vitro performance of co-electrospun PRP/poly ether sulfone/poly(vinyl) alcohol (PRP/PES/PVA) composite scaffolds for the osteogenic differentiation of human adipose-derived mesenchymal stem cells...
March 28, 2018: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
Katerina Machackova, Martina Chrudinová, Jelena Radosavljevic, Pavlo Potalitsyn, Květoslava Křížková, Milan Fábry, Irena Selicharová, Michaela Collinsová, Andrzej Marek Brzozowski, Lenka Zakova, Jiri Jiracek
Insulin-like growth factors 1 and 2 (IGF-1 and -2) are protein hormones involved in normal growth and development, but also in lifespan regulation and cancer. They exert their functions mainly through the IGF-1R receptor, or by binding to the isoform A of insulin receptor (IR-A). The development of IGF-1 and IGF-2 antagonists is of great clinical interest. Mutations of A4 and A8 sites of human insulin lead to disproportionate effects on hormone IR binding and activation. Here, we systematically modified IGF-1 sites 45, 46 and 49 and IGF-2 sites 45 and 48, which correspond, or are close, to insulin A4 and A8 sites...
April 2, 2018: Biochemistry
Matthias Widbiller, Ronald B Driesen, Andreas Eidt, Ivo Lambrichts, Karl-Anton Hiller, Wolfgang Buchalla, Gottfried Schmalz, Kerstin M Galler
INTRODUCTION: Compelling evidence pinpoints that pulp tissue engineering after the transplantation of stem cells is possible. Although intriguing, severe problems regarding clinical feasibility remain. Cell homing has been proposed as a viable alternative in which dentin-derived growth factors in a conducive scaffold may attract resident cells to form pulplike tissue. In this study, an ectopic animal model for in situ dental pulp tissue engineering was developed to evaluate whether pulplike tissue formation in empty root canals after the attraction of stem cells was possible and whether this could be enhanced by dentin-derived growth factors...
March 29, 2018: Journal of Endodontics
Liming Yin, Albert S Agustinus, Carlo Yuvienco, Takeshi Minashima, Nicole L Schnabel, Thorsten Kirsch, Jin K Montclare
Osteoarthritis (OA) results from degenerative and abnormal function of joints, with localized biochemistry playing a critical role in its onset and progression. As high levels of all-trans retinoic acid (ATRA) in synovial fluid have been identified as a contributive factor to OA, the synthesis of de novo antagonists for retinoic acid receptors (RARs) have been exploited to interrupt the mechanism of ATRA action. BMS493, a pan-RAR inverse agonist has been reported as an effective inhibitor of ATRA signaling pathway; however, it is unstable and rapidly degrades under physiological conditions...
March 30, 2018: Biomacromolecules
Francesca Diomede, Marco D'Aurora, Agnese Gugliandolo, Ilaria Merciaro, Tiziana Orsini, Valentina Gatta, Adriano Piattelli, Oriana Trubiani, Emanuela Mazzon
Bone tissue engineering is based on bone grafting to repair bone defects. Bone graft substitutes can contribute to the addition of mesenchymal stem cells (MSCs) in order to enhance the rate and the quality of defect regeneration. The stem cell secretome contains many growth factors and chemokines, which could affect cellular characteristics and behavior. Conditioned medium (CM) could be used in tissue regeneration avoiding several problems linked to the direct use of MSCs. In this study, we investigated the effect of human periodontal ligament stem cells (hPDLSCs) and their CM on bone regeneration using a commercially available membrane scaffold Evolution (EVO) implanted in rat calvarias...
March 29, 2018: International Journal of Molecular Sciences
Meenakshi Verma, Amandeep Girdhar, Basant Patel, Nirmal K Ganguly, Ritushree Kukreti, Vibha Taneja
Interactions amongst different amyloid proteins have been proposed as a probable mechanism of aggregation and thus an important risk factor for the onset as well as progression of various neurodegenerative disorders including Alzheimer's, Parkinson's, Huntington's, and Amyotrophic Lateral Sclerosis. Evidences suggest that transthyretin (TTR), a plasma protein associated with transthyretin amyloidosis or familial polyneuropathy (FAP) interacts with heterologous amyloid proteins including amyloid beta and islet amyloid polypeptide...
2018: Frontiers in Molecular Neuroscience
Mojdeh Salehi Namini, Neda Bayat, Roxana Tajerian, Somayeh Ebrahimi-Barough, Mahmoud Azami, Shiva Irani, Saranaz Jangjoo, Sadegh Shirian, Jafar Ai
BACKGROUND: An engineered tissue structure is an artificial scaffold combined with cells and signaling factors. Among various polymers, the polylactide-co-glycolide/hydroxyapatite (PLGA/HA) has attracted much attention due to their optimal properties. The aim of this study was to study the behavior of human endometrial stem cell (hEnSC)-derived osteoblast cells cultured on PLGA/HA nanocomposite scaffolds. METHODS: hEnSCs were isolated and exposed to osteogenic media for 21 days...
March 27, 2018: Journal of Orthopaedic Surgery and Research
A M Dingle, K K Yap, Y-W Gerrand, C J Taylor, E Keramidaris, Z Lokmic, A M Kong, H L Peters, W A Morrison, G M Mitchell
BACKGROUND: The liver sinusoidal capillaries play a pivotal role in liver regeneration, suggesting they may be beneficial in liver bioengineering. This study isolated mouse liver sinusoidal endothelial cells (LSECs) and determined their ability to form capillary networks in vitro and in vivo for liver tissue engineering purposes. METHODS AND RESULTS: In vitro LSECs were isolated from adult C57BL/6 mouse livers. Immunofluorescence labelling indicated they were LYVE-1+ /CD32b+ /FactorVIII+ /CD31- ...
March 26, 2018: Angiogenesis
Chao Zeng, Chi Nguyen, Scott Boitano, Jim A Field, Farhang Shadman, Reyes Sierra-Alvarez
The production and application of engineered nanoparticles (NPs) are increasing in demand with the rapid development of nanotechnology. However, there are concerns that some of these novel materials could lead to emerging environmental and health problems. Some NPs are able to facilitate the transport of contaminants into cells/organisms via a "Trojan Horse" effect which enhances the toxicity of the adsorbed materials. In this work, we evaluated the toxicity of arsenite (As(III)) adsorbed onto cerium dioxide (CeO2 ) NPs to human bronchial epithelial cells (16HBE14o-) using the xCELLigence real time cell analyzing system (RTCA)...
March 22, 2018: Environmental Research
D Vázquez-Nion, B Silva, B Prieto
Bioreceptivity is a fundamental concept in the ornamental stone industry and in the fields of cultural heritage and civil engineering to understand the susceptibility of stone constructions to biological colonisation and subsequent biodeterioration. However, a bioreceptivity index (BI) has not yet been established for any construction material. The aim of the present study is developing a simple, robust and well-founded BI for granitic rocks. For this purpose, a standardised laboratory protocol was used to grow phototrophic biofilms on several varieties of granite...
March 21, 2018: Science of the Total Environment
Nancy Staggers, Beth L Elias, Ellen Makar, Gregory L Alexander
BACKGROUND: Health information technology (IT) usability issues are a key concern for nurse executives and nurses. OBJECTIVES: The aims of this study are to understand usability pain points faced by nurses regarding the use of health IT, identify their impact and importance, discuss responsibilities, and develop possible solutions to improve the health IT-user experience for nurses. METHODS: Twenty-seven experts were interviewed including nursing leaders, informaticists, executives, engineers, researchers, and human factors experts across acute care, long-term care, and vendor settings...
April 2018: Journal of Nursing Administration
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