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Human Factors Engineering Study

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https://www.readbyqxmd.com/read/29456294/cobalt-containing-bioactive-glasses-reduce-human-mesenchymal-stem-cell-chondrogenic-differentiation-despite-hif-1%C3%AE-stabilisation
#1
E Littmann, H Autefage, A K Solanki, C Kallepitis, J R Jones, M Alini, M Peroglio, M M Stevens
Bioactive glasses (BGs) are excellent delivery systems for the sustained release of therapeutic ions and have been extensively studied in the context of bone tissue engineering. More recently, due to their osteogenic properties and expanding application to soft tissue repair, BGs have been proposed as promising materials for use at the osteochondral interface. Since hypoxia plays a critical role during cartilage formation, we sought to investigate the influence of BGs releasing the hypoxia-mimicking agent cobalt (CoBGs) on human mesenchymal stem cell (hMSC) chondrogenesis, as a novel approach that may guide future osteochondral scaffold design...
March 2018: Journal of the European Ceramic Society
https://www.readbyqxmd.com/read/29452946/time-responsive-osteogenic-niche-of-stem-cells-a-sequentially-triggered-dual-peptide-loaded-alginate-hybrid-system-for-promoting-cell-activity-and-osteo-differentiation
#2
Zuyuan Luo, Siqi Zhang, Jijia Pan, Rui Shi, Hao Liu, Yalin Lyu, Xiao Han, Yan Li, Yue Yang, Zhixiu Xu, Yi Sui, En Luo, Yuanyuan Zhang, Shicheng Wei
The efficacy of stem cell-based bone tissue engineering has been hampered by cell death and limited fate control. A smart cell culture system with the capability of sequentially delivering multiple factors in specific growth stages, like the mechanism of the natural extracellular matrix modulating tissue formation, is attractive for enhancing cell activity and controlling cell fate. Here, a bone forming peptide-1 (BFP-1)-laden mesoporous silica nanoparticles (pep@MSNs) incorporated adhesion peptide, containing the arginine-glycine-aspartic acid (RGD) domain, modified alginate hydrogel (RA) system (pep@MSNs-RA) was developed to promote the activity and stimulate osteo-differentiation of human mesenchymal stem cells (hMSCs) in sequence...
February 10, 2018: Biomaterials
https://www.readbyqxmd.com/read/29452238/human-adipose-tissue-derived-stromal-cells-in-combination-with-exogenous-stimuli-facilitate-three-dimensional-network-formation-of-human-endothelial-cells-derived-from-various-sources
#3
Dominique Manikowski, Birgit Andrée, Esther Samper, Clémence Saint-Marc, Ruth Olmer, Peter Vogt, Sarah Strauß, Axel Haverich, Andres Hilfiker
In natural tissues, the nutrition of cells and removal of waste products is facilitated by a dense capillary network which is generated during development. This perfusion system is also indispensable for tissue formation in vitro. Nutrition depending solely on diffusion is not sufficient to generate tissues of clinically relevant dimensions, which is a core aim in tissue engineering research. In this study, the establishment of a vascular network was investigated in a self-assembling approach employing endothelial and mural cells...
February 13, 2018: Vascular Pharmacology
https://www.readbyqxmd.com/read/29448134/enhancing-proliferation-and-optimizing-the-culture-condition-for-human-bone-marrow-stromal-cells-using-hypoxia-and-fibroblast-growth-factor-2
#4
Jung-Seok Lee, Seul Ki Kim, Byung-Joo Jung, Seong-Bok Choi, Eun-Young Choi, Chang-Sung Kim
This study aimed to determine the cellular characteristics and behaviors of human bone marrow stromal cells (hBMSCs) expanded in media in a hypoxic or normoxic condition and with or without fibroblast growth factor-2 (FGF-2) treatment. hBMSCs isolated from the vertebral body and expanded in these four groups were evaluated for cellular proliferation/migration, colony-forming units, cell-surface characterization, in vitro differentiation, in vivo transplantation, and gene expression. Culturing hBMSCs using a particular environmental factor (hypoxia) and with the addition of FGF-2 increased the cellular proliferation rate while enhancing the regenerative potential, modulated the multipotency-related processes (enhanced chondrogenesis-related processes/osteogenesis, but reduced adipogenesis), and increased cellular migration and collagen formation...
January 9, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29432742/knockdown-of-arl4c-inhibits-osteogenic-differentiation-of-human-adipose-derived-stem-cells-through-disruption-of-the-wnt-signaling-pathway
#5
Wensi Wang, Siyi Wang, Xuenan Liu, Ranli Gu, Yuan Zhu, Ping Zhang, Yunsong Liu, Yongsheng Zhou
ADP-ribosylation factor-like 4C (ARL4C) has been shown to play an important role in cholesterol secretion, microtubule dynamics, and cell morphological changes. However, its role in osteogenesis has not been explored. In this study, we found that ARL4C is downregulated during the osteogenic differentiation of human adipose derived stem cells (hASCs). Knockdown of ARL4C suppresses osteogenesis of hASCs in vitro and in vivo. We demonstrate that ARL4C knockdown likely attenuates osteogenesis of hASCs through inhibition of the Wnt signaling pathway...
February 9, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29428675/a-human-osteoarthritis-osteochondral-organ-culture-model-for-cartilage-tissue-engineering
#6
P Yeung, W Zhang, X N Wang, C H Yan, B P Chan
RATIONALE: In vitro human osteoarthritis (OA)-mimicking models enabling pathophysiological studies and evaluation of emerging therapies such as cartilage tissue engineering are of great importance. OBJECTIVE: We describe the development and characterization of a human OA osteochondral organ culture. We also apply this model for evaluation of the phenotype maintenance of a human MSC derived engineered cartilage, as an example of emerging therapeutics, under long term exposure to the OA-mimicking environment...
February 4, 2018: Biomaterials
https://www.readbyqxmd.com/read/29425719/cell-reprogramming-by-3d-bioprinting-of-human-fibroblasts-in-polyurethane-hydrogel-for-fabrication-of-neural-like-constructs
#7
Lin Ho, Shan-Hui Hsu
3D bioprinting is a technique which enables the direct printing of biodegradable materials with cells into 3D tissue. So far there is no cell reprogramming in situ performed with the 3D bioprinting process. Forkhead box D3 (FoxD3) is a transcription factor and neural crest marker, which was reported to reprogram human fibroblasts into neural crest stem-like cells. In this study, we synthesized a new biodegradable thermo-responsive waterborne polyurethane (PU) gel as a bioink. FoxD3 plasmids and human fibroblasts were co-extruded with the PU hydrogel through the syringe needle tip for cell reprogramming...
February 6, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29424184/-different-sources-of-mesenchymal-stem-cells-for-the-treatment-of-cartilage-repair-in-knee-joint
#8
REVIEW
Lian Wang, Peng Hou, Tao Jiang, Zong-Bao Wang, Yin-Xue Zhao, Ke Wu
As propose of organ repair stem cell therapy technology, articular cartilage cannot be repaired by itself has become one of the research hotspots, repair of articular cartilage with mesenchymal stem cells has shown obvious advantages for the treatment. The scholars have made a preliminary study on the role of mesenchymal stem cells from different sources in the repair of knee articular cartilage, and with the combination of transplantation and cartilage tissue engineering, these technologies improved the human cartilage repair effect of bone marrow, adipose, synovium, cord blood derived stem cells, which achieved good clinical curative effect...
June 25, 2017: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
https://www.readbyqxmd.com/read/29421555/synthetic-design-of-growth-factor-sequestering-extracellular-matrix-mimetic-hydrogel-for-promoting-in%C3%A2-vivo-bone-formation
#9
Hong Ji Yan, Tommaso Casalini, Gry Hulsart-Billström, Shujiang Wang, Oommen P Oommen, Matteo Salvalaglio, Sune Larsson, Jöns Hilborn, Oommen P Varghese
Synthetic scaffolds that possess an intrinsic capability to protect and sequester sensitive growth factors is a primary requisite for developing successful tissue engineering strategies. Growth factors such as recombinant human bone morphogenetic protein-2 (rhBMP-2) is highly susceptible to premature degradation and to provide a meaningful clinical outcome require high doses that can cause serious side effects. We discovered a unique strategy to stabilize and sequester rhBMP-2 by enhancing its molecular interactions with hyaluronic acid (HA), an extracellular matrix (ECM) component...
February 3, 2018: Biomaterials
https://www.readbyqxmd.com/read/29416335/human-%C3%AE-defensin-3-combined-gold-nanoparticles-for-enhancement-of-osteogenic-differentiation-of-human-periodontal-ligament-cells-in-inflammatory-microenvironments
#10
Jing Zhou, Yangheng Zhang, Lingjun Li, Huangmei Fu, Wenrong Yang, Fuhua Yan
Objective: It is a great challenge to absorb and conduct biophysicochemical interactions at the nano-bio interface. Peptides are emerging as versatile materials whose function can be programmed to perform specific tasks. Peptides combined nanoparticles might be utilized as a new approach of treatment. Human β-defensin 3 (hBD3), possesses both antimicrobial and proregeneration properties. Gold nanoparticles (AuNPs) have shown promising applications in the field of tissue engineering. However, the coordinating effects of AuNPs and hBD3 on human periodontal ligament cells (hPDLCs) remain unknown...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29415996/antitumor-activity-of-egfr-specific-car-t-cells-against-non-small-cell-lung-cancer-cells-in-vitro-and-in-mice
#11
He Li, Yao Huang, Du-Qing Jiang, Lian-Zhen Cui, Zhou He, Chao Wang, Zhi-Wei Zhang, Hai-Li Zhu, Yong-Mei Ding, Lin-Fang Li, Qiang Li, Hua-Jun Jin, Qi-Jun Qian
Effective control of non-small-cell lung cancer (NSCLC) remains clinically challenging, especially during advanced stages of the disease. This study developed an adoptive T-cell treatment through expression of a chimeric antigen receptor (CAR) to target human epidermal growth factor receptor (EGFR) in NSCLC. We optimized the non-viral piggyBac transposon system to engineer human T cells for the expression of EGFR-CAR, consisting of EGFR scFv, transmembrane domain, and intracellular 4-1BB-CD3ζ signaling domains...
February 7, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29415631/silk-biomaterials-mediated-mirna-functionalized-orthopedic-devices
#12
Eric James, Emily Van Doren, Chunmei Li, David L Kaplan
Silk-based bioresorbable medical devices, such as screws, plates and rods, have been under investigation due to their promising properties for orthopedic repairs. Options to functionalize these devices for enhanced control of bone regeneration would also exploit the compatible processing methods used to generate the devices. MicroRNAs are important regulators of bone maintenance and formation, and miRNA-based therapeutics have the potential to aid bone repair, utilizing a transient therapeutic approach with local bioactivity...
February 7, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29410245/engineered-humanized-bone-organs-maintain-human-hematopoiesis-in-vivo
#13
Kristin Fritsch, Sébastien Pigeot, Xiaomin Feng, Paul E Bourgine, Timm Schroeder, Ivan Martin, Markus G Manz, Hitoshi Takizawa
Hematopoietic stem cells (HSCs) are maintained in a specialized bone marrow (BM) environment, the so-called HSC niche, that provides pivotal factors for their maintenance. While the cellular and molecular components of the mouse BM HSC niche have been extensively studied using genetically modified animals, relatively little is known about the counterpart human BM niche components. We have previously shown with a developmental tissue engineering approach that human adult BM-derived mesenchymal stromal cells (MSCs) can develop into human bone organs (so-called ossicles) through endochondral ossification in vivo, and that these human ossicles are able to maintain functional mouse HSCs...
February 2, 2018: Experimental Hematology
https://www.readbyqxmd.com/read/29399087/simultaneous-harvesting-of-endothelial-progenitor-cells-and-mesenchymal-stem-cells-from-the-human-umbilical-cord
#14
Hao Zhang, Yanling Tao, Saisai Ren, Haihui Liu, Hui Zhou, Jiangwei Hu, Yongyong Tang, Bin Zhang, Hu Chen
The human umbilical cord (UC) is usually discarded as biological waste. However, it has attracted interest as a source of cells including endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs), which have demonstrated enormous potential in regenerative medicine. The present study describes a convenient protocol that has been developed to sequentially extract these two cell types from a single UC. EPCs which had properties of progenitor cells were successfully isolated from the UC vein. These cells had cobble-shaped morphology and expressed Flt-1, KDR, VE-cadherin, von Willebrand factor and CD31 mRNA, in addition to CD73, CD105 and vascular endothelial growth factor receptor-2...
January 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29399066/combination-therapy-of-htertr-and-fam96a-for-hepatocellular-carcinoma-through-enhancing-apoptosis-sensitivity
#15
Wan-Peng Wang, Hai-Ying Gao
Avoidance of apoptosis induced by anticancer drugs is an essential factor of carcinogenesis and a hallmark of resistance to cancer therapy. Human telomerase reverse transcriptase receptor (hTERTR) is a potential anti-cancer agent for inhibiting tumor growth. Family with sequence similarity 96 member A (FAM96A) is a ubiquitous, conserved protein and possesses apoptosome-activating and pro-apoptotic tumor suppressor potential in hepatocellular carcinoma (HCC). In the present study, hTERTR and FAM96A were identified as efficient anti-cancer agents for activating apoptosomes and reducing tumor growth...
January 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29389887/improvement-of-the-chondrocyte-specific-phenotype-upon-equine-bone-marrow-mesenchymal-stem-cell-differentiation-influence-of-culture-time-transforming-growth-factors-and-type-i-collagen-sirnas-on-the-differentiation-index
#16
Thomas Branly, Romain Contentin, Mélanie Desancé, Thibaud Jacquel, Lélia Bertoni, Sandrine Jacquet, Frédéric Mallein-Gerin, Jean-Marie Denoix, Fabrice Audigié, Magali Demoor, Philippe Galéra
Articular cartilage is a tissue characterized by its poor intrinsic capacity for self-repair. This tissue is frequently altered upon trauma or in osteoarthritis (OA), a degenerative disease that is currently incurable. Similar musculoskeletal disorders also affect horses and OA incurs considerable economic loss for the equine sector. In the view to develop new therapies for humans and horses, significant progress in tissue engineering has led to the emergence of new generations of cartilage therapy. Matrix-associated autologous chondrocyte implantation is an advanced 3D cell-based therapy that holds promise for cartilage repair...
February 1, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29387633/spheroid-formation-and-modulation-of-tenocyte-specific-gene-expression-under-simulated-microgravity
#17
Armin Kraus, Ronald Luetzenberg, Nauras Abuagela, Siri Hollenberg, Manfred Infanger
Background: For tendon tissue engineering, tenocyte-seeded scaffolds are a promising approach. Under conventional 2D culture however, tenocytes show rapid senescene and phenotype loss. We hypothesized that phenotype loss could be counteracted by simulated microgravity conditions. Methods: Human tenocytes were exposed to microgravity for 9 days on a Random Positioning Machine (RPM). Formation of 3D-structures (spheroids) was observed under light microscopy, gene expression was measured by real-time PCR...
July 2017: Muscles, Ligaments and Tendons Journal
https://www.readbyqxmd.com/read/29386903/human-centered-design-of-a-personal-health-record-system-for-metabolic-syndrome-management-based-on-the-iso-9241-210-2010-standard
#18
Charic D Farinango, Juan S Benavides, Jesús D Cerón, Diego M López, Rosa E Álvarez
Background: Previous studies have demonstrated the effectiveness of information and communication technologies to support healthy lifestyle interventions. In particular, personal health record systems (PHR-Ss) empower self-care, essential to support lifestyle changes. Approaches such as the user-centered design (UCD), which is already a standard within the software industry (ISO 9241-210:2010), provide specifications and guidelines to guarantee user acceptance and quality of eHealth systems...
2018: Journal of Multidisciplinary Healthcare
https://www.readbyqxmd.com/read/29386057/delivery-of-vegfa-in-bone-marrow-stromal-cells-seeded-in-copolymer-scaffold-enhances-angiogenesis-but-is-inadequate-for-osteogenesis-as-compared-with-the-dual-delivery-of-vegfa-and-bmp2-in-a-subcutaneous-mouse-model
#19
Sunita Sharma, Dipak Sapkota, Ying Xue, Saroj Rajthala, Mohammed A Yassin, Anna Finne-Wistrand, Kamal Mustafa
BACKGROUND: In bone tissue engineering (BTE), extensive research into vascular endothelial growth factor A (VEGFA)-mediated angiogenesis has yielded inconsistent results. The aim of this study was to investigate the influence on angio- and osteogenesis of adenoviral-mediated delivery of VEGFA alone or in combination with bone morphogenetic protein 2 (BMP2) in bone marrow stromal cells (BMSC) seeded onto a recently developed poly(LLA-co-CL) scaffold. METHODS: Human BMSC were engineered to express VEGFA alone or in combination with BMP2 and seeded onto poly(LLA-co-CL) scaffolds...
January 31, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29382315/genetic-engineering-of-escherichia-coli-to-improve-l-phenylalanine-production
#20
Yongfei Liu, Yiran Xu, Dongqin Ding, Jianping Wen, Beiwei Zhu, Dawei Zhang
BACKGROUND: L-phenylalanine (L-Phe) is an essential amino acid for mammals and applications expand into human health and nutritional products. In this study, a system level engineering was conducted to enhance L-Phe biosynthesis in Escherichia coli. RESULTS: We inactivated the PTS system and recruited glucose uptake via combinatorial modulation of galP and glk to increase PEP supply in the Xllp01 strain. In addition, the HTH domain of the transcription factor TyrR was engineered to decrease the repression on the transcriptional levels of L-Phe pathway enzymes...
January 30, 2018: BMC Biotechnology
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