keyword
MENU ▼
Read by QxMD icon Read
search

Human Factors Engineering Study

keyword
https://www.readbyqxmd.com/read/27919692/improved-production-of-2-fucosyllactose-in-engineered-escherichia-coli-by-expressing-putative-%C3%AE-1-2-fucosyltransferase-wcfb-from-bacteroides-fragilis
#1
Young-Wook Chin, Ji-Yeong Kim, Jae-Han Kim, Sang-Min Jung, Jin-Ho Seo
2'-Fucosyllactose (2-FL) is one of most abundant oligosaccharides in human milk, which is involved in many biological functions for infant health. Since 2-FL has a great potential in application to functional food materials and pharmaceuticals, several microbial systems for mass production of 2-FL have been developed in recent years. Microbial production of 2-FL was suggested to be influenced by a number of factors including fucosylation activity of α-1,2-fucosyltransferase. In the present study, the wcfB gene coding for α-1,2-fucosyltransferase from Bacteroides fragilis was screened from eleven candidates of putative α-1,2-fucosyltransferase...
December 2, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27913846/potent-mutagenicity-of-some-non-planar-tri-and-tetrachlorinated-biphenyls-in-mammalian-cells-human-cyp2e1-being-a-major-activating-enzyme
#2
Yungang Liu, Keqi Hu, Hansi Jia, Guifang Jin, Hansruedi Glatt, Hao Jiang
Polychlorinated biphenyls (PCBs) have been classified as human carcinogens. Mutagenicity of lower chlorinated biphenyls as well as activation of transcription factors by some other congeners may contribute to the carcinogenicity of PCBs. Recently, we reported that human CYP2E1 activates mono- and dichlorobiphenyls to mutagens. However, mutagenicity of other PCBs and the involvement of other CYPs remained unknown. In this study, Chinese hamster V79-derived cell lines genetically engineered for expression of individual human CYP enzymes and a human hepatocyte (L-02) line endogenously expressing various CYPs were used to determine the activities of several tri- and tetrachlorobiphenyls to induce micronuclei and gene mutations...
December 2, 2016: Archives of Toxicology
https://www.readbyqxmd.com/read/27913355/hierarchically-micro-and-nanopatterned-topographical-cues-for-modulation-of-cellular-structure-and-function
#3
Hoon Seonwoo, Won-Gyu Bae, Sunho Park, Hong-Nam Kim, Kyoung Soon Choi, Ki Taek Lim, Hoon Hyun, Jin-Woo Kim, Jangho Kim, Jong Hoon Chung
Living cells receive biochemical and physical information from the surrounding microenvironment and respond to this information. Multiscale hierarchical substrates with micro- and nanogrooves have been shown to mimic the native extracellular matrix (ECM) better than conventional nanopatterned substrates; therefore, substrates with hierarchical topographical cues are considered suitable for investigating the role of physical factors in tissue functions. In this study, precisely controllable, multiscale hierarchical substrates that could mimic the micro- and nanotopography of complex ECMs were fabricated and used to culture various cell types, including fibroblasts, endothelial cells, osteoblasts, and human mesenchymal stem cells...
December 1, 2016: IEEE Transactions on Nanobioscience
https://www.readbyqxmd.com/read/27911032/a-dermal-equivalent-engineered-with-tgf-%C3%AE-3-expressing-bone-marrow-stromal-cells-and-amniotic-membrane-cosmetic-healing-of-full-thickness-skin-wounds-in-rats
#4
Ali Samadikuchaksaraei, Ahmad Mehdipour, Mehryar Habibi Roudkenar, Javad Verdi, Mohammad Taghi Joghataei, Kamran As'adi, Fatemeh Amiri, Mozhgan Dehghan Harati, Mazaher Gholipourmalekabadi, Nushin Karkuki Osguei
Transforming growth factor beta-3 (TGF-β3) has been shown to decrease scar formation after scheduled topical applications to the cutaneous wounds. This study aimed to continuously deliver TGF-β3, during the early phase of wound healing, by engineering a dermal equivalent (DE) using TGF-β3 expressing bone marrow stromal cells (BM-SCs) and human dehydrated amniotic membrane (hDAM). To engineer a DE, rat BM-SCs were seeded on the hDAM and TGF-β3 was transiently transfected into the BM-SCs using a plasmid vector...
December 2016: Artificial Organs
https://www.readbyqxmd.com/read/27909701/immunological-quality-and-performance-of-tumor-vessel-targeting-car-t-cells-prepared-by-mrna-ep-for-clinical-research
#5
Kanako Inoo, Ryo Inagaki, Kento Fujiwara, Shigemi Sasawatari, Takashi Kamigaki, Shinsaku Nakagawa, Naoki Okada
We previously reported that tumor vessel-redirected T cells, which were genetically engineered with chimeric antigen receptor (CAR) specific for vascular endothelial growth factor receptor 2 (VEGFR2), demonstrated significant antitumor effects in various murine solid tumor models. In the present study, we prepared anti-VEGFR2 CAR-T cells by CAR-coding mRNA electroporation (mRNA-EP) and analyzed their immunological characteristics and functions for use in clinical research. The expression of anti-VEGFR2 CAR on murine and human T cells was detected with approximately 100% efficiency for a few days, after peaking 6-12 hours after mRNA-EP...
2016: Molecular Therapy Oncolytics
https://www.readbyqxmd.com/read/27908936/genome-editing-technologies-principles-and-applications
#6
REVIEW
Thomas Gaj, Shannon J Sirk, Sai-Lan Shui, Jia Liu
Targeted nucleases have provided researchers with the ability to manipulate virtually any genomic sequence, enabling the facile creation of isogenic cell lines and animal models for the study of human disease, and promoting exciting new possibilities for human gene therapy. Here we review three foundational technologies-clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9), transcription activator-like effector nucleases (TALENs), and zinc-finger nucleases (ZFNs)...
December 1, 2016: Cold Spring Harbor Perspectives in Biology
https://www.readbyqxmd.com/read/27908256/a-review-on-nano-antimicrobials-metal-nanoparticles-methods-and-mechanisms
#7
Edris Hoseinzadeh, Pouran Makhdoumi, Parisa Taha, John Stelling, Hooshyar Hossini, Mohammad Amjad Kamal, Ghulam Md Ashraf
Nanotechnology is a scientific and engineering technology conducted at the Nano-scale, such as in the fields of compound fabric manufacturing, food processing, agricultural processing, and engineering, as well as in medical and medicinal application. In recent decade, nanomaterial applications for antimicrobial works have been interested by many researchers. Available reports show that some of the metal oxide Nanoparticles including; Al2O3, TiO2, ZnO, CuO, Co3O4, In2O3, MgO, SiO2, ZrO2, Cr2O3, Ni2O3, Mn2O3, CoO, and Nickel oxide have toxicity toward several microorganisms and they could successfully kill numerous bacteria...
December 1, 2016: Current Drug Metabolism
https://www.readbyqxmd.com/read/27899508/optimized-inducible-shrna-and-crispr-cas9-platforms-for-in-vitro-studies-of-human-development-using-hpscs
#8
Alessandro Bertero, Matthias Pawlowski, Daniel Ortmann, Kirsten Snijders, Loukia Yiangou, Miguel Cardoso de Brito, Stephanie Brown, William G Bernard, James D Cooper, Elisa Giacomelli, Laure Gambardella, Nicholas R F Hannan, Dharini Iyer, Fotios Sampaziotis, Felipe Serrano, Mariëlle C F Zonneveld, Sanjay Sinha, Mark Kotter, Ludovic Vallier
Inducible loss of gene function experiments are necessary to uncover mechanisms underlying development, physiology and disease. However, current methods are complex, lack robustness and do not work in multiple cell types. Here we address these limitations by developing single-step optimized inducible gene knockdown or knockout (sOPTiKD or sOPTiKO) platforms. These are based on genetic engineering of human genomic safe harbors combined with an improved tetracycline-inducible system and CRISPR/Cas9 technology...
December 1, 2016: Development
https://www.readbyqxmd.com/read/27899064/gdf5-mediated-enhancement-of-chondrocyte-phenotype-is-inhibited-by-heparin-implication-for-the-use-of-heparin-in-the-clinic-and-in-tissue-engineering-applications
#9
Bethanie Imogen Ayerst, Raymond A A Smith, Victor Nurcombe, Anthony J Day, Catherine L R Merry, Simon Cool
The highly sulfated glycosaminoglycan (GAG) heparin is widely used in the clinic as an anticoagulant, and researchers are now using it to enhance stem cell expansion/ differentiation protocols, as well as to improve the delivery of growth factors for tissue engineering strategies. Growth differentiation factor 5 (GDF5) belongs to the bone morphogenetic protein family of proteins and is vital for skeletal formation, however, its interaction with heparin and heparan sulfate (HS) has not been studied. We identify GDF5 as a novel heparin/ HS-binding protein, and show that HS proteoglycans are vital in localizing GDF5 to the cell surface...
November 29, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27898094/crispr-cas9-aav-mediated-knock-in-at-nrl-locus-in-human-embryonic-stem-cells
#10
Xianglian Ge, Haitao Xi, Fayu Yang, Xiao Zhi, Yanghua Fu, Ding Chen, Ren-He Xu, Ge Lin, Jia Qu, Junzhao Zhao, Feng Gu
Clustered interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated genome engineering technologies are sparking a new revolution in biological research. This technology efficiently induces DNA double strand breaks at the targeted genomic sequence and results in indel mutations by the error-prone process of nonhomologous end joining DNA repair or homologous recombination with a DNA repair template. The efficiency of genome editing with CRISPR/Cas9 alone in human embryonic stem cells is still low...
November 29, 2016: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/27895039/region-specific-effect-of-the-decellularized-meniscus-extracellular-matrix-on-mesenchymal-stem-cell-based-meniscus-tissue-engineering
#11
Kazunori Shimomura, Benjamin B Rothrauff, Rocky S Tuan
BACKGROUND: The meniscus is the most commonly injured knee structure, and surgical repair is often ineffective. Tissue engineering-based repair or regeneration may provide a needed solution. Decellularized, tissue-derived extracellular matrices (ECMs) have received attention for their potential use as tissue-engineered scaffolds. In considering meniscus-derived ECMs (mECMs) for meniscus tissue engineering, it is noteworthy that the inner and outer regions of the meniscus have different structural and biochemical features, potentially directing the differentiation of cells toward region-specific phenotypes...
November 28, 2016: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/27890119/how-do-health-service-professionals-consider-human-factors-when-purchasing-interactive-medical-devices-a-qualitative-interview-study
#12
Christopher James Vincent, Ann Blandford
We present findings of a UK study into how those involved in purchasing interactive medical devices go about evaluating usability, the challenges that arise, and opportunities for improvement. The study focused on procurement of infusion devices because these are used by various professionals across healthcare. A semi-structured interview study was carried out involving a range of stakeholders (20 in total) involved in or impacted by medical device procurement. Data was analysed using thematic analysis, a qualitative method designed to support the identification, analysis and reporting of patterns...
March 2017: Applied Ergonomics
https://www.readbyqxmd.com/read/27878968/proprotein-convertase-inhibition-promotes-ciliated-cell-differentiation-a-potential-mechanism-for-the-inhibition-of-notch1-signalling-by-decanoyl-rvkr-chloromethylketone
#13
Sang-Nam Lee, In-Suk Choi, Hyun Jun Kim, Eun Jin Yang, Hyun Jin Min, Joo-Heon Yoon
Chronic repetitive rounds of injury and repair in the airway lead to airway remodelling, including ciliated cell loss and mucous cell hyperplasia. Airway remodelling is mediated by many growth and differentiation factors including Notch1, which are proteolytically processed by proprotein convertases (PCs). The present study evaluated a novel approach for controlling basal cell-type determination based on the inhibition of PCs. It was found that decanoyl-RVKR-chloromethylketone (CMK), a PC inhibitor, promotes ciliated cell differentiation and has no effect on the ciliary beat frequency in air-liquid interface (ALI) cultures of human nasal epithelial cells (HNECs)...
November 22, 2016: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/27864999/a-synthetic-toolbox-for-the-in-situ-formation-of-functionalized-homo-and-heteropolysaccharide-based-hydrogel-libraries
#14
Nick Dibbert, Andreas Krause, Julio-Cesar Rios-Camacho, Ina Gruh, Andreas Kirschning, Gerald Dräger
A synthetic toolbox for the introduction of aldehydo and hydrazido groups into the polysaccharides hyaluronic acid, alginate, dextran, pullulan, glycogen, and carboxymethyl cellulose and their use for hydrogel formation is reported. Upon mixing differently functionalized polysaccharides derived from the same natural precursor, hydrazone cross-linking takes place, which results in formation of a hydrogel composed of one type of polysaccharide backbone. Likewise, hydrogels based on two different polysaccharide strands can be formed after mixing the corresponding aldehydo- and hydrazido-modified polysaccharides...
November 16, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27861930/scaffold-free-cartilage-tissue-engineering-with-a-small-population-of-human-nasoseptal-chondrocytes
#15
Loraine L Y Chiu, William T H To, John M Lee, Stephen D Waldman
OBJECTIVE: Cartilage tissue engineering is a promising approach to provide suitable materials for nasal reconstruction; however, it typically requires large numbers of cells. We have previously shown that a small number of chondrocytes cultivated within a continuous flow bioreactor can elicit substantial tissue growth, but translation to human chondrocytes is not trivial. Here, we aimed to demonstrate the application of the bioreactor to generate large-sized tissues from a small population of primary human nasoseptal chondrocytes...
November 15, 2016: Laryngoscope
https://www.readbyqxmd.com/read/27856281/adsorbed-fibrinogen-stimulates-tlr-4-on-monocytes-and-induces-bmp-2-expression
#16
Marta I Oliveira, Marta L Pinto, Raquel M Gonçalves, M Cristina L Martins, Susana G Santos, Mário A Barbosa
: Modulation of inflammatory responses to implanted biomaterials towards tissue regeneration has gained prominence as an innovative tissue engineering strategy. Recent in vitro and in vivo studies showed that Fibrinogen (Fg) adsorbed to Chitosan (Ch) substrates modulates immune cell responses, enhances the production of osteogenic factors by monocytes/macrophages and promotes bone regeneration, but the mechanisms involved remain poorly understood. Thus, the present work was conducted to clarify the molecular mechanisms of interaction between primary human monocytes and the above substrates...
November 14, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27834208/expanding-the-host-range-of-hepatitis-c-virus-through-viral-adaptation
#17
Markus von Schaewen, Marcus Dorner, Kathrin Hueging, Lander Foquet, Sherif Gerges, Gabriela Hrebikova, Brigitte Heller, Julia Bitzegeio, Juliane Doerrbecker, Joshua A Horwitz, Gisa Gerold, Sebastian Suerbaum, Charles M Rice, Philip Meuleman, Thomas Pietschmann, Alexander Ploss
: Hepatitis C virus (HCV) species tropism is incompletely understood. We have previously shown that at the level of entry, human CD81 and occludin (OCLN) comprise the minimal set of human factors needed for viral uptake into murine cells. As an alternative approach to genetic humanization, species barriers can be overcome by adapting HCV to use the murine orthologues of these entry factors. We previously generated a murine tropic HCV (mtHCV or Jc1/mCD81) strain harboring three mutations within the viral envelope proteins that allowed productive entry into mouse cell lines...
November 8, 2016: MBio
https://www.readbyqxmd.com/read/27831902/local-and-transient-gene-expression-primes-the-liver-to-resist-cancer-metastasis
#18
Tyler J Goodwin, Yingqiu Zhou, Sara N Musetti, Rihe Liu, Leaf Huang
The liver is the primary site of metastasis for gastrointestinal cancers and is a location highly susceptible to the establishment of metastasis in numerous other primary cancers, including breast, lung, and pancreatic cancers. The current standard of care typically consists of primary tumor resection and systemic administration of potent but toxic chemotherapeutics, yielding a minimal improvement in the median survival rate. CXCL12, a chemokine, is a key factor for activating the migration/survival pathways of CXCR4(+) cancer cells and for recruiting immunosuppressive cells to areas of inflammation...
November 9, 2016: Science Translational Medicine
https://www.readbyqxmd.com/read/27830536/virus-like-particles-derived-from-hiv-1-for-delivery-of-nuclear-proteins-improvement-of-production-and-activity-by-protein-engineering
#19
Marc-André Robert, Viktoria Lytvyn, Francis Deforet, Rénald Gilbert, Bruno Gaillet
Virus-like particles (VLPs) derived from retroviruses and lentiviruses can be used to deliver recombinant proteins without the fear of causing insertional mutagenesis to the host cell genome. In this study we evaluate the potential of an inducible lentiviral vector packaging cell line for VLP production. The Gag gene from HIV-1 was fused to a gene encoding a selected protein and it was transfected into the packaging cells. Three proteins served as model: the green fluorescent protein and two transcription factors-the cumate transactivator (cTA) of the inducible CR5 promoter and the human Krüppel-like factor 4 (KLF4)...
November 9, 2016: Molecular Biotechnology
https://www.readbyqxmd.com/read/27830445/tissue-engineering-for-neurodegenerative-diseases-using-human-amniotic-membrane-and-umbilical-cord
#20
Anahí Sanluis-Verdes, Namibia Sanluis-Verdes, María Jesús Manso-Revilla, Antonio Manuel Castro-Castro, Jorge Pombo-Otero, María Fraga-Mariño, Jacinto Sanchez-Ibañez, Nieves Doménech, María Esther Rendal-Vázquez
Regenerative medicine, based on the use of stem cells, scaffolds and growth factors, has the potential to be a good approach for restoring damaged tissues of the central nervous system. This study investigated the use of human amniotic mesenchymal stem cells (hAMSC), human amniotic epithelial stem cells (hAESC), and human Wharton's jelly mesenchymal stem cells (hWJMSC) derived from human umbilical cord as a source of stem cells, and the potential of the human amniotic membrane (HAM) as a scaffold and/or source of growth factors to promote nerve regeneration...
November 9, 2016: Cell and Tissue Banking
keyword
keyword
92771
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"