Julieta Ramírez-Cuéllar, Roberto Ferrari, Rosario T Sanz, Marta Valverde-Santiago, Judith García-García, A Silvina Nacht, David Castillo, Francois Le Dily, Maria Victoria Neguembor, Marco Malatesta, Sarah Bonnin, Marc A Marti-Renom, Miguel Beato, Guillermo P Vicent
The cancer epigenome has been studied in cells cultured in two-dimensional (2D) monolayers, but recent studies highlight the impact of the extracellular matrix and the three-dimensional (3D) environment on multiple cellular functions. Here, we report the physical, biochemical, and genomic differences between T47D breast cancer cells cultured in 2D and as 3D spheroids. Cells within 3D spheroids exhibit a rounder nucleus with less accessible, more compacted chromatin, as well as altered expression of ~2000 genes, the majority of which become repressed...
April 2, 2024: EMBO Journal