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https://www.readbyqxmd.com/read/29774292/evaluation-of-phytosynthesised-silver-nanoparticles-from-leaf-extracts-of-leucas-aspera-and-hyptis-suaveolens-and-their-larvicidal-activity-against-malaria-dengue-and-filariasis-vectors
#1
Devan Elumalai, Maduraiveeran Hemavathi, Chandrasekar Vijayalakshmi Deepaa, Patheri Kunyil Kaleena
The present study deals with the green synthesis of silver nanoparticle from the aqueous leaf extracts of Leucas aspera and Hyptis suaveolens as reducing agent and to investigate the larvicidal activity of synthesized silver nanoparticles. The synthesized silver nanoparticles were characterized by Ultraviolet and visible absorption spectroscopy (UV), Fourier transform-infrared spectroscopy (FT-IR), X-ray spectroscopy (XRD), Field emission scanning electron microscope (FESEM) and High-resonance transmission electron microscopy (HRTEM) analysis...
November 2017: Parasite Epidemiology and Control
https://www.readbyqxmd.com/read/29768491/plasmodium-falciparum-dipeptidyl-aminopeptidase-3-activity-is-important-for-efficient-erythrocyte-invasion-by-the-malaria-parasite
#2
Christine Lehmann, Michele Ser Ying Tan, Laura E de Vries, Ilaria Russo, Mateo Isidrio Sanchez, Daniel E Goldberg, Edgar Deu
Parasite egress from infected erythrocytes and invasion of new red blood cells are essential processes for the exponential asexual replication of the malaria parasite. These two tightly coordinated events take place in less than a minute and are in part regulated and mediated by proteases. Dipeptidyl aminopeptidases (DPAPs) are papain-fold cysteine proteases that cleave dipeptides from the N-terminus of protein substrates. DPAP3 was previously suggested to play an essential role in parasite egress. However, little is known about its enzymatic activity, intracellular localization, or biological function...
May 16, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29763668/evaluation-of-automated-loop-mediated-amplification-lamp-for-routine-malaria-detection-in-blood-samples-of-german-travelers-a-cross-sectional-study
#3
Hagen Frickmann, Rebecca Hinz, Sandra Rojak, Insa Bonow, Stefanie Ruben, Christine Wegner, Iris Zielke, Ralf Matthias Hagen, Egbert Tannich
BACKGROUND: We assessed a commercial loop-mediated amplification (LAMP) platform for its reliability as a screening tool for malaria parasite detection. METHODS: A total of 1000 blood samples from patients with suspected or confirmed malaria submitted to the German National Reference Center for Tropical Pathogens were subjected to LAMP using the Meridian illumigene Malaria platform. Results were compared with microscopy from thick and thin blood films in all cases...
May 12, 2018: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/29760095/volatile-biomarkers-of-symptomatic-and-asymptomatic-malaria-infection-in-humans
#4
Consuelo M De Moraes, Caroline Wanjiku, Nina M Stanczyk, Hannier Pulido, James W Sims, Heike S Betz, Andrew F Read, Baldwyn Torto, Mark C Mescher
Malaria remains among the world's deadliest diseases, and control efforts depend critically on the availability of effective diagnostic tools, particularly for the identification of asymptomatic infections, which play a key role in disease persistence and may account for most instances of transmission but often evade detection by current screening methods. Research on humans and in animal models has shown that infection by malaria parasites elicits changes in host odors that influence vector attraction, suggesting that such changes might yield robust biomarkers of infection status...
May 14, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29754588/evaluating-seroprevalence-to-circumsporozoite-protein-to-estimate-exposure-to-three-species-of-plasmodium-in-the-brazilian-amazon
#5
Virginia Araujo Pereira, Juan Camilo Sánchez-Arcila, Mariana Pinheiro Alves Vasconcelos, Amanda Ribeiro Ferreira, Lorene de Souza Videira, Antonio Teva, Daiana Perce-da-Silva, Maria Teresa Queiroz Marques, Luzia Helena de Carvalho, Dalma Maria Banic, Luiz Cristóvão Sobrino Pôrto, Joseli Oliveira-Ferreira
BACKGROUND: Brazil has seen a great decline in malaria and the country is moving towards elimination. However, for eventual elimination, the control program needs efficient tools in order to monitor malaria exposure and transmission. In this study, we aimed to evaluate whether seroprevalence to the circumsporozoite protein (CSP) is a good tool for monitoring the exposure to and/or evaluating the burden and distribution of Plasmodium species in the Brazilian Amazon. METHODS: Cross-sectional surveys were conducted in a rural area of Porto Velho, Rondônia state...
May 14, 2018: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/29733338/persistent-plasmodium-falciparum-infection-in-women-with-an-intent-to-become-pregnant-is-a-risk-factor-for-pregnancy-associated-malaria
#6
Nicaise Tuikue Ndam, Bernard Tornyigah, Akpéyédjé Yannelle Dossou, Guillaume Escriou, Morten A Nielsen, Ali Salanti, Saadou Issifou, Achille Massougbodji, Jean-Philippe Chippaux, Philippe Deloron
Background: Pregnant women are more susceptible to P. falciparum than before pregnancy, and infection has consequences for both mother and offspring. WHO recommends that pregnant woman in areas of transmission receive intermittent preventive treatment starting in the second trimester. Consequently, women are not protected during the first trimester, although P. falciparum infections are both frequent and harmful. Methods: A cohort of nulligravidae women was followed during subsequent pregnancy...
May 4, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29728152/infection-of-anopheles-aquasalis-from-symptomatic-and-asymptomatic-plasmodium-vivax-infections-in-manaus-western-brazilian-amazon
#7
Keillen M Martins-Campos, Andrea Kuehn, Anne Almeida, Ana Paula M Duarte, Vanderson S Sampaio, Íria C Rodriguez, Sara G M da Silva, Claudia María Ríos-Velásquez, José Bento Pereira Lima, Paulo Filemon Paolucci Pimenta, Quique Bassat, Ivo Müller, Marcus Lacerda, Wuelton M Monteiro, Maria das Graças V Barbosa Guerra
BACKGROUND: Asymptomatic individuals are one of the major challenges for malaria elimination programs in endemic areas. In the absence of clinical symptoms and with a lower parasite density they constitute silent reservoirs considered important for maintaining transmission of human malaria. Studies from Brazil have shown that infected individuals may carry these parasites for long periods. RESULTS: Patients were selected from three periurban endemic areas of the city of Manaus, in the western Brazilian Amazon...
May 4, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29724225/towards-development-of-aptamers-that-specifically-bind-to-lactate-dehydrogenase-of-plasmodium-falciparum-through-epitopic-targeting
#8
Kelly-Anne Frith, Ronen Fogel, J P Dean Goldring, Robert G E Krause, Makobetsa Khati, Heinrich Hoppe, Mary E Cromhout, Meesbah Jiwaji, Janice L Limson
BACKGROUND: Early detection is crucial for the effective treatment of malaria, particularly in those cases infected with Plasmodium falciparum. There is a need for diagnostic devices with the capacity to distinguish P. falciparum from other strains of malaria. Here, aptamers generated against targeted species-specific epitopes of P. falciparum lactate dehydrogenase (rPfLDH) are described. RESULTS: Two classes of aptamers bearing high binding affinity and specificity for recombinant P...
May 3, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29724210/drug-use-in-the-management-of-uncomplicated-malaria-in-public-health-facilities-in-the-democratic-republic-of-the-congo
#9
Nsengi Y Ntamabyaliro, Christian Burri, Didier B Nzolo, Aline B Engo, Yves N Lula, Samuel M Mampunza, Célestin N Nsibu, Gauthier K Mesia, Jean-Marie N Kayembe, Joris L Likwela, Leon M Kintaudi, Gaston L Tona
BACKGROUND: Malaria the first causes of death from parasitic infection worldwide. Interventions to reduce the burden of malaria have produced a tremendous drop in malaria morbidity and mortality. However, progress is slower in DRC, which shares with Nigeria 39% of deaths related to malaria globally. Inappropriate use of drugs may be one of the factors of this below-average performance. The aim of this study was to describe the use of drugs in the management of uncomplicated malaria in public health facilities in DRC...
May 3, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29723168/malaria-surveillance-united-states-2015
#10
Kimberly E Mace, Paul M Arguin, Kathrine R Tan
PROBLEM/CONDITION: Malaria in humans is caused by intraerythrocytic protozoa of the genus Plasmodium. These parasites are transmitted by the bite of an infective female Anopheles species mosquito. The majority of malaria infections in the United States occur among persons who have traveled to regions with ongoing malaria transmission. However, malaria is occasionally acquired by persons who have not traveled out of the country through exposure to infected blood products, congenital transmission, laboratory exposure, or local mosquitoborne transmission...
May 4, 2018: MMWR. Surveillance Summaries: Morbidity and Mortality Weekly Report. Surveillance Summaries
https://www.readbyqxmd.com/read/29720136/prevalence-and-predictors-of-under-nutrition-among-school-children-in-a-rural-south-eastern-nigerian-community-a-cross-sectional-study
#11
Rufina N B Ayogu, Ifeoma C Afiaenyi, Edith U Madukwe, Elizabeth A Udenta
BACKGROUND: School children in developing countries like Nigeria are faced with numerous nutrition and health problems. Lack of functional school health and nutrition programmes in Enugu state, Nigeria may be associated with dearth of data on associated factors. Identifying these factors could inform the design and implementation of school-based programmes aimed at ameliorating these problems. METHODS: A cross sectional survey involving 450 primary and secondary school children aged 6-15 years was conducted in Ede-Oballa, a rural community in Enugu state, South-eastern Nigeria...
May 2, 2018: BMC Public Health
https://www.readbyqxmd.com/read/29713592/advances-in-malaria-elimination-in-botswana-a-dramatic-shift-to-parasitological-diagnosis-2008-2014
#12
K Moakofhi, J K Edwards, M Motlaleng, J Namboze, W Butt, M Obopile, T Mosweunyane, M Manzi, K C Takarinda, P Owiti
Background: Malaria elimination requires infection detection using quality assured diagnostics and appropriate treatment regimens. Although Botswana is moving towards malaria elimination, reports of unconfirmed cases may jeopardise this effort. This study aimed to determine the proportion of cases treated for malaria that were confirmed by rapid diagnostic testing (RDT) and/or microscopy. Methods: This was a retrospective descriptive study using routine national data from the integrated disease surveillance and case-based surveillance systems from 2008 to 2014...
April 25, 2018: Public Health Action
https://www.readbyqxmd.com/read/29713586/case-management-of-malaria-in-swaziland-2011-2015-on-track-for-elimination
#13
S V Dlamini, R J Kosgei, N Mkhonta, Z Zulu, K Makadzange, S Zhou, P Owiti, W Sikhondze, J Namboze, A Reid, S Kunene
Objective: To assess adherence to malaria diagnosis and treatment guidelines (2010 and 2014) in all health care facilities in Swaziland between 2011 and 2015. Methods: This was a cross-sectional descriptive study involving all health care facilities that diagnosed and managed malaria cases in Swaziland. Patients' age, sex, diagnosis method and type of treatment were analysed. Results: Of 1981 records for severe and uncomplicated malaria analysed, 56% of cases were uncomplicated and 14% had severe malaria. The type of malaria was not recorded for 30% of cases...
April 25, 2018: Public Health Action
https://www.readbyqxmd.com/read/29703200/characterization-of-plasmodium-falciparum-and-plasmodium-vivax-recent-exposure-in-an-area-of-significantly-decreased-transmission-intensity-in-central-vietnam
#14
Johanna Helena Kattenberg, Annette Erhart, Minh Hieu Truong, Eduard Rovira-Vallbona, Khac Anh Dung Vu, Thi Hong Ngoc Nguyen, Van Hong Nguyen, Van Van Nguyen, Melanie Bannister-Tyrrell, Michael Theisen, Adam Bennet, Andrew A Lover, Thanh Duong Tran, Xuan Xa Nguyen, Anna Rosanas-Urgell
BACKGROUND: In Vietnam, malaria transmission has been reduced to very low levels over the past 20 years, and as a consequence, the country aims to eliminate malaria by 2030. This study aimed to characterize the dynamics and extent of the parasite reservoir in Central Vietnam, in order to further target elimination strategies and surveillance. METHODS: A 1-year prospective cohort study (n = 429) was performed in three rural communities in Quang Nam province...
April 27, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29695527/development-and-evaluation-of-a-novel-lamp-assay-for-the-diagnosis-of-cutaneous-and-visceral-leishmaniasis
#15
Emily Rebecca Adams, Gerard Schoone, Inge Versteeg, Maria Adelaida Gomez, Ermias Diro, Yasuyoshi Mori, Desiree Perlee, Tim Downing, Nancy Saravia, Ashenafi Assaye, Asrat Hailu, Audrey Albertini, Joseph Mathu Ndung'u, Henk Schallig
INTRODUCTION: A novel Pan-Leishmania LAMP assay was developed for diagnosis of Cutaneous and Visceral Leishmaniasis (CL & VL) which can be used in near-patient settings. METHODS: Primers were designed on the 18S rDNA and the conserved region of minicircle kDNA selected on the basis of high copy number. LAMP assays were evaluated for CL in a prospective cohort trial of 105 patients in South-West Colombia. Lesion swab samples from CL suspects were collected and tested using LAMP and compared to a composite reference of microscopy AND/OR culture to calculate diagnostic accuracy...
April 25, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29692310/gametocyte-development-and-carriage-in-ghanaian-individuals-with-uncomplicated-plasmodium-falciparum-malaria
#16
Bismarck Dinko, Felix Ansah, Comfort Agyare-Kwabi, Senyo Tagboto, Linda Eva Amoah, Britta C Urban, Colin J Sutherland, Gordon A Awandare, Kim C Williamson, Fred N Binka, Kirk W Deitsch
Plasmodium falciparum gametocytes develop over 9-12 days while sequestered in deep tissues. On emergence into the bloodstream, they circulate for varied amounts of time during which certain host factors might influence their further development. We aimed to evaluate the potential association of patient clinical parameters with gametocyte development and carriage via in vivo methods. Seventy-two patients were enrolled from three hospitals in the Volta region of Ghana in 2016. Clinical parameters were documented for all patients, and gametocyte prevalence by microscopy was estimated at 12...
April 23, 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/29690913/distribution-of-plasmodium-spp-infection-in-asymptomatic-carriers-in-perennial-and-low-seasonal-malaria-transmission-settings-in-west-africa
#17
Constant G N Gbalégba, Hampâté Ba, Kigbafori D Silué, Ousmane Ba, Emmanuel Tia, Mouhamadou Chouaibou, Nathan T Y Tian-Bi, Grégoire Y Yapi, Brama Koné, Jürg Utzinger, Benjamin G Koudou
BACKGROUND: Since 2000, substantial progress has been made in reducing malaria worldwide. However, some countries in West Africa remain a hotspot for malaria with all age groups at risk. Asymptomatic carriers of Plasmodium spp. are important sources of infections for malaria vectors and thus contribute to the anchoring of the disease in favourable eco-epidemiological settings. The objective of this study was to assess the asymptomatic malaria case rates in Korhogo and Kaedi, two urban areas in northern Côte d'Ivoire and southern Mauritania, respectively...
April 25, 2018: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/29688491/a-case-of-delayed-diagnosis-of-east-african-trypanosomiasis-in-a-dutch-traveller
#18
C A Wijsman, J L J Hanssen, H Scheper, L G Visser, L van Lieshout
We present a case of East-African trypanosomiasis (EAT) in a 56-year-old Dutch woman returning from holiday in Tanzania and Kenya. The diagnosis was delayed due to the lack of suspicion and secondly because of postponed analysis of blood microscopy after negative rapid malaria antigen testing. Second stage trypanosomiasis was ruled out with liquor analysis. She was treated first with pentamidine and shortly thereafter with suramin, after which she recovered. We emphasize the use of thin/thick smear diagnostics in travellers returning from endemic countries...
January 1, 2018: Journal of Travel Medicine
https://www.readbyqxmd.com/read/29676064/machine-learning-based-in-line-holographic-sensing-of-unstained-malaria-infected-rbcs
#19
Taesik Go, Jun Ho Kim, Hyeokjun Byeon, Sang Joon Lee
Accurate and immediate diagnosis of malaria is important for medication of the infectious disease. Conventional methods for diagnosing malaria are time consuming and rely on the skill of experts. Therefore, an automatic and simple diagnostic modality is essential for healthcare in developing countries that lack the expertise of trained microscopists. In the present study, a new automatic sensing method using digital in-line holographic microscopy (DIHM) combined with machine learning algorithms was proposed to sensitively detect unstained malaria-infected red blood cells (RBCs)...
April 19, 2018: Journal of Biophotonics
https://www.readbyqxmd.com/read/29669942/cxcl10-stabilizes-t-cell-brain-endothelial-cell-adhesion-leading-to-the-induction-of-cerebral-malaria
#20
Elizabeth W Sorensen, Jeffrey Lian, Aleksandra J Ozga, Yoshishige Miyabe, Sophina W Ji, Shannon K Bromley, Thorsten R Mempel, Andrew D Luster
Malaria remains one of the world's most significant human infectious diseases and cerebral malaria (CM) is its most deadly complication. CM pathogenesis remains incompletely understood, hindering the development of therapeutics to prevent this lethal complication. Elevated levels of the chemokine CXCL10 are a biomarker for CM, and CXCL10 and its receptor CXCR3 are required for experimental CM (ECM) in mice, but their role has remained unclear. Using multiphoton intravital microscopy, CXCR3 receptor- and ligand-deficient mice and bone marrow chimeric mice, we demonstrate a key role for endothelial cell-produced CXCL10 in inducing the firm adhesion of T cells and preventing their cell detachment from the brain vasculature...
April 19, 2018: JCI Insight
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