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https://www.readbyqxmd.com/read/29335000/molecular-characterization-of-babesia-microti-thioredoxin-bmtrx2-and-its-expression-patterns-induced-by-antiprotozoal-drugs
#1
Jingwei Huang, Kang Xiong, Houshuang Zhang, Yanzhen Zhao, Jie Cao, Haiyan Gong, Yongzhi Zhou, Jinlin Zhou
BACKGROUND: Human babesiosis is an infectious disease that is epidemic in various regions all over the world. The predominant causative pathogen of this disease is the intra-erythrocytic parasite Babesia microti. The thioredoxin system is one of the major weapons that is used in the resistance to the reactive oxygen species (ROS) and reactive nitrogen species (RNS) produced by host immune system. In other intra-erythrocytic apicomplexans like the malaria parasite Plasmodium falciparum, anti-oxidative proteins are promising targets for the development of anti-parasitic drugs...
January 15, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29332155/prevalence-of-the-pfdhfr-and-pfdhps-mutations-among-asymptomatic-pregnant-women-in-southeast-nigeria
#2
Ekpereonne Esu, Costanza Tacoli, Prabhanjan Gai, Nicole Berens-Riha, Michael Pritsch, Thomas Loescher, Martin Meremikwu
Sulfadoxine-pyrimethamine (SP) is the recommended drug for intermittent preventive treatment of malaria in pregnancy in most of sub-Saharan Africa. Resistance to SP is related to mutations in the dhfr and dhps gene of Plasmodium falciparum. This study determined the prevalence of Pfdhfr and Pfdhps polymorphisms found in asymptomatic pregnant women attending antenatal care in Calabar, Nigeria. From October 2013 to November 2014, asymptomatic pregnant women attending antenatal care clinics were enrolled after obtaining informed consent...
January 13, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29327915/multispectral-atomic-force-microscopy-infrared-nano-imaging-of-malaria-infected-red-blood-cells
#3
David Perez-Guaita, Kamila Kochan, Mitchell Batty, Christian Doerig, Jose Garcia-Bustos, Shirley Josefina Espinoza Herrera, Don McNaughton, Philip Heraud, Bayden R Wood
Atomic Force Microscopy-Infrared (AFM-IR) spectroscopy is a powerful new technique that can be applied to study molecular composition of cells and tissues at the nano-scale. AFM-IR maps are acquired using single wavenumber value: they show either the absorbance plotted against a single wavenumber value, or a ratio of two absorbance values. Here we implement multivariate image analysis to generate multivariate AFM-IR maps, and use this approach to resolve subcellular structural information in red blood cells infected with Plasmodium falciparum at different stages of development...
January 12, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29321025/diagnostic-challenges-of-prolonged-post-treatment-clearance-of-plasmodium-nucleic-acids-in-a-pre-transplant-autosplenectomized-patient-with-sickle-cell-disease
#4
Paul M Luethy, Sean C Murphy, Annette M Seilie, Yingda L Xie, Chuen-Yen Lau, John F Tisdale, Matthew M Hsieh, Jessica L Reinhardt, Anna F Lau, Gary A Fahle
BACKGROUND: Autosplenectomy, as a result of sickle cell disease, is an important risk factor for severe malaria. While molecular methods are helpful in providing rapid and accurate infection detection and species identification, the effect of hyposplenism on result interpretation during the course of infection should be carefully considered. CASE PRESENTATION: A 32-year old autosplenectomized Nigerian male with severe sickle cell disease was referred to the National Institutes of Health for allogenic hematopoietic stem cell transplant...
January 10, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29318942/high-prevalence-of-dihydrofolate-reductase-gene-mutations-in-plasmodium-falciparum-parasites-among-pregnant-women-in-nigeria-after-reported-use-of-sulfadoxine-pyrimethamine
#5
Olusola Ojurongbe, Christian N Nguetse, Samuel A Fayemiwo, Catherine O Falade, Taiwo A Ojurongbe, Bolaji N Thomas, Christian G Meyer, Thirumalaisamy P Velavan
This study assesses the prevalence of asymptomatic Plasmodium falciparum parasitemia positivity and P. falciparum dihydrofolate reductase (pfdhfr) mutations in parasite isolates among pregnant women in Southwest Nigeria. Plasmodium falciparum parasitemia was confirmed by microscopy and nested PCR in 200 pregnant women attending antenatal care. The prevalence of pfdhfr polymorphisms was determined by direct sequencing of the gene fragments containing the C50R, N51I, C59R, S108N, and I164L mutations. Information on the use of antimalarial drugs and methods applied to prevent malaria were obtained by a questionnaire...
January 10, 2018: Pathogens and Global Health
https://www.readbyqxmd.com/read/29317138/evaluation-of-the-cellavision-dm96-advanced-rbc-application-for-screening-and-follow-up-of-malaria-infection
#6
Lisa Florin, Karel Maelegheer, Astrid Muyldermans, Marjan Van Esbroeck, Eric Nulens, Jan Emmerechts
CellaVision DM96 is a digital cell morphology system for automated classification of white and red blood cells. CellaVision Advanced RBC application (ARBCA) pre-classifies RBC in 21 categories, including parasitized RBC, and allows re-classification by the operator. In this study, the performance of the software for detection of malaria and calculation of parasitemia was evaluated and compared to microscopy (n=40). For CellaVision, both pre- and post-reclassification results were evaluated. Sensitivity was moderate, even post-reclassification (72%), due to low numbers of analyzed RBC and limited resolution of photographs...
December 8, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29310580/evaluation-of-fluorescent-in-situ-hybridization-technique-for-diagnosis-of-malaria-in-ahero-sub-county-hospital-kenya
#7
Regina Kandie, Rachel Ochola, Kariuki Njaanake
BACKGROUND: Malaria is a major cause of morbidity and mortality. Treatment of malaria in a timely manner could avert deaths. Treatment ultimately relies on the rapid and accurate diagnosis. Fluorescence in situ hybridization (FISH), a cytogenetic technique based on detection of specific nucleic acid, has the potential to address the limitations of the current diagnostic approaches. This study investigates further the performance of FISH for the diagnosis of malaria in a rural setting in Western Kenya...
January 8, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29305880/distinct-effects-of-hiv-protease-inhibitors-and-erad-inhibitors-on-zygote-to-ookinete-transition-of-the-malaria-parasite
#8
Evi Goulielmaki, Sofia Kaforou, Kannan Venugopal, Thanasis G Loukeris, Inga Siden-Kiamos, Konstantinos Koussis
In an effort to eradicate malaria, new interventions are proposed to include compound/vaccine development against pre-erythrocytic, erythrocytic and mosquito stages of Plasmodium. Drug repurposing might be an alternative approach to new antimalarials reducing the cost and the time required for drug development. Previous in vitro studies have examined the effects of protease inhibitors on different stages of the Plasmodium parasite, although the clinical relevance of this remains unclear. In this study we tested the putative effect of three HIV protease inhibitors, two general aspartyl protease inhibitors and three AAA-p97 ATPase inhibitors on the zygote to ookinete transition of the Plasmodium parasite...
January 3, 2018: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29304870/dynamics-of-anti-msp3-and-pfs230-antibody-responses-and-multiplicity-of-infection-in-asymptomatic-children-from-southern-ghana
#9
Linda E Amoah, Festus K Acquah, Ruth Ayanful-Torgby, Akua Oppong, Joana Abankwa, Evans K Obboh, Susheel K Singh, Michael Theisen
BACKGROUND: During a Plasmodium infection, exposure of human host immune cells to both the asexual and the sexual stages of the parasite elicit immune responses. These responses may be protective and prevent the development of high parasitaemia and its associated clinical symptoms, or block the transmission of malaria to an uninfected person. This study aimed at examining the dynamics of naturally acquired immune responses against the asexual and sexual forms of Plasmodium falciparum as well as assessing differences in the multiplicity of infection (MOI) in asymptomatic Ghanaian children living in two communities with varying malaria transmission intensities...
January 5, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29304809/estimation-on-local-transmission-of-malaria-by-serological-approach-under-low-transmission-setting-in-myanmar
#10
Myat Htut Nyunt, Than Naing Soe, Thinzar Shein, Ni Ni Zaw, Soe Soe Han, Fauzi Muh, Seong-Kyun Lee, Jin-Hee Han, Ji-Hoon Park, Kwon-Soo Ha, Won Sun Park, Seok-Ho Hong, Myat Phone Kyaw, Eun-Taek Han
BACKGROUND: As the prevalence of the malaria has been decreasing in many endemic countries including Myanmar, malaria elimination in Greater Mekong Region was targeted not later than 2030. The relevance of molecular and serological tools to identify residual transmission remains to be established in this setting. METHODS: One-year cohort study was conducted and sera samples were collected in every 3 months with active and passive case detection for clinical malaria episodes by RDT, microscopy and molecular method...
January 5, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29304786/unclear-association-between-levels-of-plasmodium-falciparum-lactate-dehydrogenase-pfldh-in-saliva-of-malaria-patients-and-blood-parasitaemia-diagnostic-implications
#11
Eva A Nambati, William C Kiarie, Francis Kimani, James H Kimotho, Maureen S Otinga, Edwin Too, Stephen Kaniaru, Janice Limson, Wallace Bulimo
BACKGROUND: The use of saliva in diagnosis of infectious diseases is an attractive alternative to procedures that involve blood drawing. It promises to reduce risks associated with accidental needle pricks and improve patient compliance particularly in malaria survey and drug efficacy studies. Quantification of parasitaemia is useful in establishing severity of disease and in assessing individual patient response to treatment. In current practice, microscopy is the recommended technique, despite its limitations...
January 5, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29304258/the-relative-contribution-of-symptomatic-and-asymptomatic-plasmodium-vivax-and-plasmodium-falciparum-infections-to-the-infectious-reservoir-in-a-low-endemic-setting-in-ethiopia
#12
Fitsum G Tadesse, Hannah C Slater, Wakweya Chali, Karina Teelen, Kjerstin Lanke, Mulualem Belachew, Temesgen Menberu, Girma Shumie, Getasew Shitaye, Lucy C Okell, Wouter Graumans, Geert-Jan van Gemert, Soriya Kedir, Addisu Tesfaye, Feleke Belachew, Wake Abebe, Hassen Mamo, Robert Sauerwein, Taye Balcha, Abraham Aseffa, Delenasaw Yewhalaw, Endalamaw Gadisa, Chris Drakeley, Teun Bousema
Background: The majority of P. vivax and P. falciparum infections in low-endemic settings are asymptomatic. The relative contribution to the infectious reservoir of these infections, often of low-parasite-density, compared to clinical malaria cases, is currently unknown but important for malaria elimination strategies. Methods: We assessed infectivity of passively-recruited symptomatic malaria patients (n=41) and community-recruited asymptomatic individuals with microscopy- (n=41) and PCR-detected infections (n=82) using membrane feeding assays with Anopheles arabiensis mosquitoes in Adama, Ethiopia...
January 3, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29291234/a-clinical-report-on-mixed-infection-of-malaria-dengue-and-chikungunya-from-new-delhi-india
#13
Mohd Abdullah, Ayesha Tazeen, Malik Hisamuddin, Irshad H Naqvi, Hirday N Verma, Anwar Ahmed, Shama Parveen
Arthropod-borne infections like malaria, dengue and chikungunya fever are transmitted by mosquitoes. The present report describes an unusual case of mixed infection with malaria, dengue and chikungunya viruses in a 21 year old male patient from New Delhi, India during monsoon season of 2016. The malarial fever was diagnosed by thin slide microscopy and antigen test. Chikungunya virus IgM was detected in the sample by the card test. Dengue and chikungunya viruses were further confirmed by RT-PCR for CprM and E1 gene respectively...
December 2017: Virusdisease
https://www.readbyqxmd.com/read/29261647/the-detection-of-cryptic-plasmodium-infection-among-villagers-in-attapeu-province-lao-pdr
#14
Moritoshi Iwagami, Sengdeuane Keomalaphet, Phonepadith Khattignavong, Pheovaly Soundala, Lavy Lorphachan, Emilie Matsumoto-Takahashi, Michel Strobel, Daniel Reinharz, Manisack Phommasansack, Bouasy Hongvanthong, Paul T Brey, Shigeyuki Kano
BACKGROUND: Although the malaria burden in the Lao PDR has gradually decreased, the elimination of malaria by 2030 presents many challenges. Microscopy and malaria rapid diagnostic tests (RDTs) are used to diagnose malaria in the Lao PDR; however, some studies have reported the prevalence of sub-microscopic Plasmodium infections or asymptomatic Plasmodium carriers in endemic areas. Thus, highly sensitive detection methods are needed to understand the precise malaria situation in these areas...
December 20, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29251261/diagnostic-accuracy-of-loop-mediated-isothermal-amplification-lamp-for-screening-patients-with-imported-malaria-in-a-non-endemic-setting
#15
Camille Ponce, Flora Kaczorowski, Thomas Perpoint, Patrick Miailhes, Alain Sigal, Etienne Javouhey, Yves Gillet, Laurent Jacquin, Marion Douplat, Karim Tazarourte, Véronique Potinet, Bruno Simon, Adeline Lavoignat, Guillaume Bonnot, Fatimata Sow, Anne-Lise Bienvenu, Stéphane Picot
BACKGROUND: Sensitive and easy-to-perform methods for the diagnosis of malaria are not yet available. Improving the limit of detection and following the requirements for certification are issues to be addressed in both endemic and non-endemic settings. The aim of this study was to test whether loop-mediated isothermal amplification of DNA (LAMP) may be an alternative to microscopy or real-time PCR for the screening of imported malaria cases in non-endemic area. RESULTS: 310 blood samples associated with 829 suspected cases of imported malaria were tested during a one year period...
2017: Parasite: Journal de la Société Française de Parasitologie
https://www.readbyqxmd.com/read/29240337/nucleic-acid-diagnostic-approaches-in-parasitology
#16
Anne-Marie Kerttula, Antti Lavikainen
Nucleic acid diagnostic technologies are partly replacing traditional microscopy and antigen detection methods in parasitological diagnostics. In particular, the diagnostics of parasitic diarrhea is undergoing a transformation due to the application of polymerase chain reaction (PCR) tests. Diagnostics of malaria is still based on microscopy, but rapid nucleic acid tests are emerging. Laboratories of clinical microbiology in Finland currently provide PCR tests e.g. for intestinal protozoa, Toxoplasma and Trichomonas...
2017: Duodecim; Lääketieteellinen Aikakauskirja
https://www.readbyqxmd.com/read/29220497/post-treatment-hrp2-clearance-in-patients-with-uncomplicated-plasmodium-falciparum-malaria
#17
Mateusz M Plucinski, Pedro Rafael Dimbu, Filomeno Fortes, Salim Abdulla, Saumu Ahmed, Julie Gutman, S Patrick Kachur, Aida Badiane, Daouda Ndiaye, Eldin Talundzic, Naomi Lucchi, Michael Aidoo, Venkatachalam Udhayakumar, Eric Halsey, Eric Rogier
Background: Response to antimalarial treatment is assessed using serial microscopy. New techniques for accurate measurement of the Plasmodium falciparum histidine rich protein 2 (HRP2) antigen have made monitoring antigen concentration over time a potential alternative for assessing treatment response. Methods: Post-treatment HRP2 concentration was measured in longitudinal samples from 537 participants with P. falciparum malaria from efficacy trials in Angola, Tanzania, and Senegal...
December 6, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29217951/five-years-malaria-trend-analysis-in-woreta-health-center-northwest-ethiopia
#18
Awoke Derbie, Megbaru Alemu
Background: An estimated 68% of the Ethiopian population, living in 75% of the landmass, is at risk of contracting malaria at any time making it the leading public health problem. The temporal analysis of malaria data could be important to evaluate the performance of malaria prevention programmes. Thus, the aim of this study was to determine the trend of malaria at Woreta Health Center (WHC) over a period of five years. Methods: We analyzed the records of 8,057 presumptive malaria patients registered in 2012 to 2016...
September 2017: Ethiopian Journal of Health Sciences
https://www.readbyqxmd.com/read/29217380/evaluation-of-rapid-pfhrp-2-pldh-based-tests-in-diagnosing-microscopy-confirmed-falciparum-malaria-in-hodeidah-governorate-yemen
#19
Ahmed A Azazy, Ali Jamaly Alhawery, Rashad Abdul-Ghani, R A Alharbi, Shaia S R Almalki
Along with the determination of malaria infection rate among suspected patients attending hospitals in Hodeidah governorate, the present study evaluated the accuracy of Plasmodium falciparum histidine-rich protein-2 (PfHRP-2)/parasite-specific lactate dehydrogenase (pLDH)-based rapid diagnostic test (RDT) for the diagnosis of microscopy-confirmed falciparum malaria. An overall malaria infection rate of 19.3% (57/295) among suspected patients attending hospitals was microscopically confirmed. The sensitivity of thin blood films for the detection of malaria parasites was 79...
December 5, 2017: Acta Tropica
https://www.readbyqxmd.com/read/29210351/seasonal-dynamics-of-malaria-in-pregnancy-in-west-africa-evidence-for-carriage-of-infections-acquired-before-pregnancy-until-first-contact-with-antenatal-care
#20
Isha Berry, Patrick Walker, Harry Tagbor, Kalifa Bojang, Sheick Oumar Coulibaly, Kassoum Kayentao, John Williams, Abraham Oduro, Paul Milligan, Daniel Chandramohan, Brian Greenwood, Matthew Cairns
In malaria-endemic areas, Plasmodium falciparum prevalence is often high in young women because of 1) low use of insecticide-treated nets before their first pregnancy and 2) acquired immunity, meaning infections are asymptomatic and thus untreated. Consequently, a common source of malaria in pregnancy (MiP) may be infected women becoming pregnant, rather than pregnant women becoming infected. In this study, prevalence of infection was determined by microscopy at first antenatal care (ANC) visit in primigravidae and secundigravidae in Ghana, Burkina Faso, Mali, and The Gambia, four countries with strong seasonal variations in transmission...
December 4, 2017: American Journal of Tropical Medicine and Hygiene
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