keyword
MENU ▼
Read by QxMD icon Read
search

Dnam

keyword
https://www.readbyqxmd.com/read/29433075/glucocorticoid-receptor-gene-methylation-moderates-the-association-of-childhood-trauma-and-cortisol-stress-reactivity
#1
Nina Alexander, Clemens Kirschbaum, Matthis Wankerl, Benjamin J Stauch, Tobias Stalder, Susann Steudte-Schmiedgen, Markus Muehlhan, Robert Miller
Exposure to childhood trauma (CT) has been linked to sustained dysregulations of major stress response systems, including findings of both exaggerated and attenuated hypothalamus-pituitary-adrenal (HPA) axis activity. Likewise, CT constitutes a common risk factor for a broad range of psychiatric conditions that involve distinct neuroendocrine profiles. In this study, we investigated the role of epigenetic variability in a stress-related gene as a potential mediator or moderator of such differential trajectories in CT survivors...
January 31, 2018: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/29415463/changes-in-dna-methylation-from-age-18-to-pregnancy-in-type-1-2-and-17-t-helper-and-regulatory-t-cells-pathway-genes
#2
Sabrina Iqbal, Gabrielle A Lockett, John W Holloway, S Hasan Arshad, Hongmei Zhang, Akhilesh Kaushal, Sabarinath R Tetali, Nandini Mukherjee, Wilfried J J Karmaus
To succeed, pregnancies need to initiate immune biases towards T helper 2 (Th2) responses, yet little is known about what establishes this bias. Using the Illumina 450 K platform, we explored changes in DNA methylation (DNAm) of Th1, Th2, Th17, and regulatory T cell pathway genes before and during pregnancy. Female participants were recruited at birth (1989), and followed through age 18 years and their pregnancy (2011-2015). Peripheral blood DNAm was measured in 245 girls at 18 years; from among these girls, the DNAm of 54 women was repeatedly measured in the first (weeks 8-21, n = 39) and second (weeks 22-38, n = 35) halves of pregnancy, respectively...
February 6, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29399631/dna-methylation-as-a-mediator-of-the-association-between-prenatal-adversity-and-risk-factors-for-metabolic-disease-in-adulthood
#3
Elmar W Tobi, Roderick C Slieker, René Luijk, Koen F Dekkers, Aryeh D Stein, Kate M Xu, P Eline Slagboom, Erik W van Zwet, L H Lumey, Bastiaan T Heijmans
Although it is assumed that epigenetic mechanisms, such as changes in DNA methylation (DNAm), underlie the relationship between adverse intrauterine conditions and adult metabolic health, evidence from human studies remains scarce. Therefore, we evaluated whether DNAm in whole blood mediated the association between prenatal famine exposure and metabolic health in 422 individuals exposed to famine in utero and 463 (sibling) controls. We implemented a two-step analysis, namely, a genome-wide exploration across 342,596 cytosine-phosphate-guanine dinucleotides (CpGs) for potential mediators of the association between prenatal famine exposure and adult body mass index (BMI), serum triglycerides (TG), or glucose concentrations, which was followed by formal mediation analysis...
January 2018: Science Advances
https://www.readbyqxmd.com/read/29383163/reduced-representation-bisulfite-sequencing-rrbs-of-dairy-goat-mammary-glands-reveals-dna-methylation-profiles-of-integrated-genome-wide-and-critical-milk-related-genes
#4
Xiaoyan Zhang, Sihuan Zhang, Lin Ma, Enhui Jiang, Han Xu, Rui Chen, Qing Yang, Hong Chen, Zhuanjian Li, Xianyong Lan
DNA methylation (DNAm), a major element of epigenetics, plays critical roles in individual development. Reduced representation bisulfite sequencing (RRBS) is an effective and economical method for analyzing the DNA methylation of a single base. The aims of this study were to determine the DNAm profiles of the methylation contexts (CGs and non-CGs) of lactation and dry periods of goat mammary glands using the RRBS, and to identify potential milk-related genes. The proportion of CG was the highest among all the sequence contexts...
December 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29373860/cadmium-associated-differential-methylation-throughout-the-placental-genome-epigenome-wide-association-study-of-two-u-s-birth-cohorts
#5
Todd M Everson, Tracy Punshon, Brian P Jackson, Ke Hao, Luca Lambertini, Jia Chen, Margaret R Karagas, Carmen J Marsit
BACKGROUND: Cadmium (Cd) is a ubiquitous environmental toxicant that can accumulate in the placenta during pregnancy, where it may impair placental function and affect fetal development. OBJECTIVES: We aimed to investigate Cd-associated variations in placental DNA methylation (DNAM) and associations with gene expression; we also aimed to identify novel pathways involved in Cd-associated reproductive toxicity. METHODS: Using placental DNAM and Cd concentrations in the New Hampshire Birth Cohort Study (NHBCS, n=343) and the Rhode Island Child Health Study (RICHS, n=141), we performed an epigenome-wide association study (EWAS) between Cd and DNAM, adjusting for tissue heterogeneity using a reference-free method...
January 22, 2018: Environmental Health Perspectives
https://www.readbyqxmd.com/read/29370095/innovative-clinical-perspectives-for-cik-cells-in-cancer-patients
#6
REVIEW
Martino Introna, Fabio Correnti
Cytokine-induced killer (CIK) cells are T lymphocytes that have acquired, in vitro, following extensive manipulation by Interferon gamma (IFN-γ), OKT3 and Interleukin 2 (IL-2) addition, the expression of several Natural Killer (NK) cell-surface markers. CIK cells have a dual "nature", due to the presence of functional TCR as well as NK molecules, even if the antitumoral activity can be traced back only to the NK-like structures (DNAM-1, NKG2D, NKp30 and CD56). In addition to antineoplastic activity in vitro and in several in-vivo models, CIK cells show very limited, if any, GvHD toxicity as well as a strong intratumoral homing...
January 25, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29367468/dna-methylation-signatures-reflect-aging-in-patients-with-nonalcoholic-steatohepatitis
#7
Rohit Loomba, Yevgeniy Gindin, Zhaoshi Jiang, Eric Lawitz, Stephen Caldwell, C Stephen Djedjos, Ren Xu, Chuhan Chung, Robert P Myers, G Mani Subramanian, Zachary Goodman, Michael Charlton, Nezam H Afdhal, Anna Mae Diehl
A DNA methylation (DNAm) signature (the "Horvath clock") has been proposed as a measure of human chronological and biological age. We determined peripheral blood DNAm in patients with nonalcoholic steatohepatitis (NASH) and assessed whether accelerated aging occurs in these patients. DNAm signatures were obtained in patients with biopsy-proven NASH and stage 2-3 fibrosis. The DNAm profile from one test and two validation cohorts served as controls. Age acceleration was calculated as the difference between DNAm age and the predicted age based on the linear model derived from controls...
January 25, 2018: JCI Insight
https://www.readbyqxmd.com/read/29358174/low-density-lipoprotein-uptake-inhibits-the-activation-and-antitumour-functions-of-human-vgamma9vdelta2-t-cells
#8
Neidy Varela Rodrigues, Daniel V Correia, Sofia Mensurado, Sandrina Nobrega-Pereira, Ana deBarros, Fernanda Kyle-Cezar, Andrew Tutt, Adrian C Hayday, Haakan Norell, Bruno Silva-Santos, Sergio Dias
Vgamma9Vdelta2 T cells, the main subset of gamma delta T lymphocytes in human peripheral blood, are endowed with antitumor functions such as cytotoxicity and IFNgamma production. These functions are triggered upon TCR-dependent activation by non-peptidic prenyl pyrophosphates ("phosphoantigens") that are selective agonists of Vgamma9Vdelta2 T cells, and which have been evaluated in clinical studies. Because phosphoantigens have shown inter-individual variation in Vgamma9Vdelta2 T-cell activities, we asked whether metabolic resources, namely lipids such as cholesterol, could affect phosphoantigen-mediated Vgamma9Vdelta2 T-cell activation and function...
January 22, 2018: Cancer Immunology Research
https://www.readbyqxmd.com/read/29338330/joint-influence-of-snps-and-dna-methylation-on-lipids-in-african-americans-from-hypertensive-sibships
#9
Michelle L Wright, Erin B Ware, Jennifer A Smith, Sharon L R Kardia, Jacquelyn Y Taylor
INTRODUCTION: Plasma concentrations of lipids (i.e., total cholesterol, high-density cholesterol, low-density cholesterol, and triglycerides) are amenable to therapeutic intervention and remain important factors for assessing risk of cardiovascular diseases. Some of the observed variability in serum lipid concentrations has been associated with genetic and epigenetic variants among cohorts with European ancestry (EA). Serum lipid levels have also been associated with genetic variants in multiethnic populations...
January 1, 2018: Biological Research for Nursing
https://www.readbyqxmd.com/read/29298087/quantitative-evaluation-of-mmp-9-and-timp-1-promoter-methylation-in-chronic-periodontitis
#10
Xiting Li, Jiaxuan Lu, Wei Teng, Chuanjiang Zhao, Xiaolei Ye
In this study, we investigated the promoter DNA methylation (DNAm) status of the MMP-9 and TIMP-1 genes in patients with chronic periodontitis to evaluate disease progression. Using pyrosequencing technology, DNAm levels of MMP-9 and TIMP-1 CpG islands were measured in 88 chronic periodontitis patients and 15 healthy controls. We found a positive correlation between methylation levels of MMP-9 CpG islands and the severity of chronic periodontitis. Methylated CpG islands were also closely associated with the duration of chronic periodontitis...
January 3, 2018: DNA and Cell Biology
https://www.readbyqxmd.com/read/29296800/the-number-of-cd56dim-nk-cells-in-the-graft-has-a-major-impact-on-risk-of-disease-relapse-following-allo-hsct
#11
Luke Maggs, Francesca Kinsella, Y L Tracey Chan, Suzy Eldershaw, Duncan Murray, Jane Nunnick, Joanne Bird, Charles Craddock, Jianmin Zuo, Ram Malladi, Paul Moss
The graft-versus-leukemia (GVL) effect of allogeneic hemopoietic stem cell transplantation (allo-HSCT) is mediated by the donor immune system and acts to decrease the rate of disease relapse. Although studies of posttransplant immune reconstitution have identified correlates of clinical outcome, the number and profile of mature immune cells infused with the stem cell graft is also likely to be an important determinant and has been relatively poorly studied. We characterized immune cells within the stem cell graft of 107 patients who underwent T-cell-depleted allo-HSCT and related this to clinical outcome...
August 22, 2017: Blood Advances
https://www.readbyqxmd.com/read/29273273/utility-of-dna-methylation-to-assess-placental-health
#12
Samantha L Wilson, Wendy P Robinson
DNA methylation (DNAm), a mitotically stable epigenetic mark, can influence as well as reflect gene expression. DNAm has been gaining interest for use as a biomarker for many conditions including placental insufficiency, specifically preeclampsia (PE) and intrauterine growth restriction (IUGR). Additionally, DNAm may retain a "memory" of earlier in utero exposures and hence provide insight into pathogeneses occurring earlier in gestation. This review will discuss the placental DNA methylome, the uses of DNAm to assess placental health, and considerations and limitations to understand in epigenome-wide association studies (EWAS)...
December 14, 2017: Placenta
https://www.readbyqxmd.com/read/29258399/perceived-racial-discrimination-and-dna-methylation-among-african-american-women-in-the-intergen-study
#13
Veronica Barcelona de Mendoza, Yunfeng Huang, Cindy A Crusto, Yan V Sun, Jacquelyn Y Taylor
INTRODUCTION: Experiences of racial discrimination have been associated with poor health outcomes. Little is known, however, about how perceived racial discrimination influences DNA methylation (DNAm) among African Americans (AAs). We examined the association of experiences of discrimination with DNAm among AA women in the Intergenerational Impact of Genetic and Psychological Factors on Blood Pressure (InterGEN) study. METHODS: The InterGEN study examines the effects of genetic and psychological factors on blood pressure among AA women and their children...
January 1, 2017: Biological Research for Nursing
https://www.readbyqxmd.com/read/29225347/accelerated-epigenetic-aging-and-mitochondrial-dna-copy-number-in-bipolar-disorder
#14
Gabriel R Fries, Isabelle E Bauer, Giselli Scaini, Mon-Ju Wu, Iram F Kazimi, Samira S Valvassori, Giovana Zunta-Soares, Consuelo Walss-Bass, Jair C Soares, Joao Quevedo
Bipolar disorder (BD) has been previously associated with accelerated aging; yet, the mechanisms underlying this association are largely unknown. The epigenetic clock has been increasingly recognized as a valuable aging marker, although its association with other biological clocks in BD patients and high-risk subjects, such as telomere length and mitochondrial DNA (mtDNA) copy number, has never been investigated. We included 22 patients with BD I, 16 siblings of BD patients, and 20 healthy controls in this analysis...
December 11, 2017: Translational Psychiatry
https://www.readbyqxmd.com/read/29202043/epstein-barr-virus-induces-adhesion-receptor-cd226-dnam-1-expression-during-primary-b-cell-transformation-into-lymphoblastoid-cell-lines
#15
Lisa Grossman, Chris Chang, Joanne Dai, Pavel A Nikitin, Dereje D Jima, Sandeep S Dave, Micah A Luftig
Epstein-Barr virus (EBV), an oncogenic herpesvirus, infects and transforms primary B cells into immortal lymphoblastoid cell lines (LCLs), providing a model for EBV-mediated tumorigenesis. EBV transformation stimulates robust homotypic aggregation, indicating that EBV induces molecules that mediate cell-cell adhesion. We report that EBV potently induced expression of the adhesion molecule CD226, which is not normally expressed on B cells. We found that early after infection of primary B cells, EBV promoted an increase in CD226 mRNA and protein expression...
November 2017: MSphere
https://www.readbyqxmd.com/read/29189780/a-semi-synthetic-organism-that-stores-and-retrieves-increased-genetic-information
#16
Yorke Zhang, Jerod L Ptacin, Emil C Fischer, Hans R Aerni, Carolina E Caffaro, Kristine San Jose, Aaron W Feldman, Court R Turner, Floyd E Romesberg
Since at least the last common ancestor of all life on Earth, genetic information has been stored in a four-letter alphabet that is propagated and retrieved by the formation of two base pairs. The central goal of synthetic biology is to create new life forms and functions, and the most general route to this goal is the creation of semi-synthetic organisms whose DNA harbours two additional letters that form a third, unnatural base pair. Previous efforts to generate such semi-synthetic organisms culminated in the creation of a strain of Escherichia coli that, by virtue of a nucleoside triphosphate transporter from Phaeodactylum tricornutum, imports the requisite unnatural triphosphates from its medium and then uses them to replicate a plasmid containing the unnatural base pair dNaM-dTPT3...
November 29, 2017: Nature
https://www.readbyqxmd.com/read/29182664/differential-inhibitory-and-activating-nk-cell-receptor-levels-and-nk-nkt-like-cell-functionality-in-chronic-and-recovered-stages-of-chikungunya
#17
Subrat Thanapati, Mohini A Ganu, Anuradha S Tripathy
The role of natural killer (NK; CD3-CD56+)/NKT (CD3+CD56+)-like cells in chikungunya virus (CHIKV) disease progression/recovery remains unclear. Here, we investigated the expression profiles and function of NK and NKT-like cells from 35 chronic chikungunya patients, 30 recovered individuals, and 69 controls. Percentage of NKT-like cells was low in chronic chikungunya patients. NKp30+, CD244+, DNAM-1+, and NKG2D+ NK cell percentages were also lower (MFI and/or percentage), while those of CD94+ and NKG2A+ NKT-like cells were higher (MFI and/or percentage) in chronic patients than in recovered subjects...
2017: PloS One
https://www.readbyqxmd.com/read/29175600/forensic-dna-methylation-profiling-from-minimal-traces-how-low-can-we-go
#18
Jana Naue, Huub C J Hoefsloot, Ate D Kloosterman, Pernette J Verschure
Analysis of human DNA methylation (DNAm) can provide additional investigative leads in crime cases, e.g. the type of tissue or body fluid, the chronological age of an individual, and differentiation between identical twins. In contrast to the genetic profile, the DNAm level is not the same in every cell. At the single cell level, DNAm represents a binary event at a defined CpG site (methylated versus non-methylated). The DNAm level from a DNA extract however represents the average level of methylation of the CpG of interest of all molecules in the forensic sample...
November 13, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/29162165/epigenetic-correlates-of-neonatal-contact-in-humans
#19
Sarah R Moore, Lisa M McEwen, Jill Quirt, Alex Morin, Sarah M Mah, Ronald G Barr, W Thomas Boyce, Michael S Kobor
Animal models of early postnatal mother-infant interactions have highlighted the importance of tactile contact for biobehavioral outcomes via the modification of DNA methylation (DNAm). The role of normative variation in contact in early human development has yet to be explored. In an effort to translate the animal work on tactile contact to humans, we applied a naturalistic daily diary strategy to assess the link between maternal contact with infants and epigenetic signatures in children 4-5 years later, with respect to multiple levels of child-level factors, including genetic variation and infant distress...
December 2017: Development and Psychopathology
https://www.readbyqxmd.com/read/29159506/a-longitudinal-study-of-dna-methylation-as-a-potential-mediator-of-age-related-diabetes-risk
#20
Crystal D Grant, Nadereh Jafari, Lifang Hou, Yun Li, James D Stewart, Guosheng Zhang, Archana Lamichhane, JoAnn E Manson, Andrea A Baccarelli, Eric A Whitsel, Karen N Conneely
DNA methylation (DNAm) has been found to show robust and widespread age-related changes across the genome. DNAm profiles from whole blood can be used to predict human aging rates with great accuracy. We sought to test whether DNAm-based predictions of age are related to phenotypes associated with type 2 diabetes (T2D), with the goal of identifying risk factors potentially mediated by DNAm. Our participants were 43 women enrolled in the Women's Health Initiative. We obtained methylation data via the Illumina 450K Methylation array on whole blood samples from participants at three timepoints, covering on average 16 years per participant...
November 20, 2017: GeroScience
keyword
keyword
92737
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"