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https://www.readbyqxmd.com/read/28434202/distribution-of-norovirus-genotypes-and-subtypes-in-river-water-by-ultra-deep-sequencing-based-analysis
#1
M Boonchan, K Motomura, K Inoue, H Ode, P Y Chu, M Lin, Y Iwatani, K Ruchusatsawat, R Guntapong, R Tacharoenmuang, S Chantaroj, M Tatsumi, N Takeda, S Sangkitporn
To determine the distribution of NoV genotypes in natural river water in Thailand, we conducted a genome analysis using a next-generation sequencer. Twenty-five river water samples were collected at five different sites of the Khlong Klon River in the suburbs of Bangkok between August 2013 and December 2014. The partial genome of NoV was detected in 15 of the 25 samples (60.0%). Seven of these 15 samples (46.7%) contained multiple NoV GII genotypes: GII.4, GII.6, and GII.17. Our data showed that GII.17 had already emerged in August 2013 as a minor population, and it became a major genotype in December 2014...
April 23, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28434154/protective-effects-of-cell-free-supernatant-and-live-lactic-acid-bacteria-isolated-from-thai-pigs-against-a-pandemic-strain-of-porcine-epidemic-diarrhea-virus
#2
Wandee Sirichokchatchawan, Gun Temeeyasen, Dachrit Nilubol, Nuvee Prapasarakul
Porcine epidemic diarrhea virus (PEDV) is a coronavirus which causes severe diarrhea and fatal dehydration in piglets. In general, probiotic supplements could enhance recovery and protect piglets against enteric pathogens. Seven local lactic acid bacteria (LAB), (Ent. faecium 79N and 40N, Lact. plantarum 22F, 25F and 31F, Ped. acidilactici 72N and Ped. pentosaceus 77F) from pig feces were well-characterized as high potential probiotics. Cell-free supernatants (CFS) and live LAB were evaluated for antiviral activities by co-incubation on Vero cells and challenged with a pandemic strain of PEDV isolated from pigs in Thailand...
April 22, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28433572/descriptions-of-the-female-and-larva-of-simulium-gomphostilbia-udomi-diptera-simuliidae-from-thailand-and-its-transfer-to-the-simulium-asakoae-species-group
#3
Atiporn Saeung, Wichai Srisuka, Van Lun Low, Wanchai Maleewong, Hiroyuki Takaoka
The female and larva of Simulium (Gomphostilbia) udomi Takaoka & Choochote from Thailand are described for the first time. The female of this species is similar to those of S. (G.) asakoae Takaoka & Davies from Peninsular Malaysia, Thailand, Hong Kong and Vietnam, and S. (G.) chiangdaoense Takaoka & Srisuka from Thailand. The larva of this species is similar to S. (G.) curtatum Jitklang et al. and S. (G.) nr. asakoae 2 from Thailand in having a medium-long postgenal cleft. Taxonomic notes are given to separate this species from these related species...
April 19, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28431957/genetic-study-of-kir-and-hla-ligands-in-235-individuals-from-northeastern-thailand
#4
Suwit Chaisri, Chanvit Leelayuwat, Amornrat Romphruk
The diversity of 17 KIR and HLA ligands (HLA-C1, C2, Bw4, A11) were investigated in two hundred and thirty-five unrelated healthy donors in Northeastern Thais (NETs) by the polymerase chain reaction with sequence-specific primer (PCR-SSP) method. The Hardy-Weinberg Equilibrium (HWE) was used to verify genotyping method for dimorphic KIR and HLA. They were in HWE (p>0.05). KIR and HLA ligands frequencies, genotypes, haplotypes and linkage disequilibrium (LD) were presented. The genetic data are available in allele Frequencies Net Database...
April 18, 2017: Human Immunology
https://www.readbyqxmd.com/read/28427794/occurrence-of-a-novel-class-1-integron-harboring-qnrvc4-in-salmonella-rissen
#5
Sirirat Luk-In, Chaiwat Pulsrikarn, Aroon Bangtrakulnonth, Tanittha Chatsuwan, Wanla Kulwichit
We described qnrVC4 in S. Rissen 166ANSS50, a swine isolate, which was detected in the study on quinolone resistance mechanisms of nontyphoidal Salmonella in Thailand. The isolate was found to harbor a ̴17-kb non-conjugative plasmid carrying qnrVC4 within 8.91kb of a novel In4-like class 1 integron (In805). It contained the multi-drug resistance gene cassettes of qnrVC4-qacH4-aacA4-cmlA7-blaOXA-10-aadA1-dfrA14 and unusual 3'-CS of mobC-IS6100. This 1014-bp qnrVC4 cassette included with promoter (PqnrVC4: -35 TTGAGA and -10 TAGTCT) showed high homology with qnrVC4 in superintegron of V...
March 31, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28426113/health-problems-among-thai-tourists-returning-from-india
#6
Jutarmas Olanwijitwong, Watcharapong Piyaphanee, Kittiyod Poovorawan, Saranath Lawpoolsri, Pornthep Chanthavanich, Pongdej Wichainprasast, Terapong Tantawichien
Background: The number of Thai tourists visiting India is increasing each year. Most studies investigating health problems among international travellers to India have focused on travellers from Europe or North America, and the applicability of these studies to Asian travellers is unknown. Methods: This cross-sectional study used data collected from Thai tourists who had recently completed a trip to India. A questionnaire on demographic data, travel characteristics, pre-travel health preparation, and health problems during the trip to India was administered...
July 1, 2017: Journal of Travel Medicine
https://www.readbyqxmd.com/read/28424253/identification-of-acute-hiv-1-infection-by-hologic-aptima-hiv-1-rna-qualitative-assay
#7
Mark M Manak, Leigh Anne Eller, Jennifer Malia, Linda L Jagodzinski, Rapee Trichavaroj, Joseph Oundo, Cornelia Lueer, Fatim Cham, Mark de Souza, Nelson L Michael, Merlin L Robb, Sheila A Peel
The Hologic Aptima HIV-1 Qualitative RNA assay was used in a rigorous screening approach designed to identify individuals at the earliest stage of HIV-1 infection for enrollment into subsequent studies of cellular and viral events in early infection (RV217/Early Capture HIV Cohort - ECHO Study). Volunteers at high risk of HIV-1 infection were recruited from study sites in Thailand, Tanzania, Uganda and Kenya with high HIV-1 prevalence rates among the populations examined. Small volume blood samples were collected by finger-stick at twice-weekly intervals and tested by Aptima...
April 19, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28423884/integrating-market-chain-assessments-with-zoonoses-risk-analysis-in-two-cross-border-pig-value-chains-in-lao-pdr
#8
Anna Okello, Tassilo Tamino Tiemann, Phouth Inthavong, Boualam Khamlome, Ammaly Phengvilaysouk, Soukanh Keonouchanh, Chattouphone Keokhamphet, Virasack Somoulay, Kate Blaszak, Stuart Blacksell, Walter Okello, John Allen
Lao PDR's recent accession to the World Trade Organization necessitates a greater understanding of the patterns and risk of livestock production in order to better align national policy with the Agreement on the Application of Sanitary and Phytosanitary Measures. This eco-health study describes the integration of market chain and zoonoses risk analyses at two strategic cross border points between Lao PDR, Thailand and Viet Nam. Information gained from smallholder farmer/trader interviews was integrated with serological surveys for pig-associated zoonoses - including hepatitis E virus, Taenia solium and Trichinella - to identify potential linkages between disease risk and pig production and slaughter in low input systems common across the country...
March 25, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28422611/predictors-of-hearing-protection-use-among-industrial-workers
#9
Kunlayanee Tantranont, Nuntanat Codchanak
Promoting the use of hearing protection devices (HPDs) can prevent noise-induced hearing loss (NIHL) among workers who are exposed to excessive noise. In the present study, the authors examine factors that may explain HPD use among industrial workers from 15 manufacturing plants in Thailand. Participants consisted of 268 randomly selected workers exposed to harmful noise levels for which routine HPD use was required. Logistic regression analysis of study variables revealed the most powerful predictors of HPD use were perceived hearing status ( b = 0...
April 1, 2017: Workplace Health & Safety
https://www.readbyqxmd.com/read/28422580/glyphosate-and-paraquat-in-maternal-and-fetal-serums-in-thai-women
#10
Pornpimol Kongtip, Noppanun Nangkongnab, Ratanavadee Phupancharoensuk, Chonlada Palarach, Dusit Sujirarat, Supha Sangprasert, Malasod Sermsuk, Namthip Sawattrakool, Susan Renee Woskie
OBJECTIVES: This longitudinal study measured the glyphosate and paraquat concentrations found in maternal and umbilical cord serum in 82 pregnant women who gave birth in three provinces of Thailand. METHODS: Through questionnaires and biological samples collected at childbirth, factors such as personal characteristics, occupation, agricultural activities, and herbicide use in agricultural work were evaluated as predictors of glyphosate and paraquat levels in the pregnant women...
April 19, 2017: Journal of Agromedicine
https://www.readbyqxmd.com/read/28422120/trends-of-increases-in-potential-risk-factors-and-prevalence-rates-of-diabetes-mellitus-in-thailand
#11
REVIEW
V Chavasit, W Kriengsinyos, J Photi, K Tontisirin
Over the past three decades, undernutrition in Thailand has drastically reduced by over seven times. However, since 1995 the number of patients afflicted with non-communicable diseases, such as diabetes mellitus, has rapidly increased, even among the young. Unhealthy life styles due to urbanization are a major reason for this increase. Less physical activity and low consumption of fruits and vegetables, as well as high consumption of added free sugar, are common. Every year, the Thai people increase their consumption of energy from fat and protein, while lowering their intake of energy from complex carbohydrates...
April 19, 2017: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28422081/non-responsive-feeding-practices-unhealthy-eating-behaviors-and-risk-of-child-overweight-and-obesity-in-southeast-asia-a-systematic-review
#12
REVIEW
Ana Cristina Lindsay, Somporn Sitthisongkram, Mary L Greaney, Sherrie F Wallington, Praewrapee Ruengdej
Childhood obesity is increasing dramatically in many Southeast Asian countries, and becoming a significant public health concern. This review summarizes the evidence on associations between parental feeding practices, child eating behaviors, and the risk of overweight and obesity in Southeast Asian children 2-12 years old. We systematically searched five electronic academic/research (PubMed, PsycINFO, ProQuest Nursing, Medline, and CINAHL) databases using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement for peer-reviewed studies published in English between January 2000 and December 2016...
April 19, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28420389/assessment-of-copy-number-variation-in-genes-related-to-drug-resistance-in-plasmodium-vivax-and-plasmodium-falciparum-isolates-from-the-brazilian-amazon-and-a-systematic-review-of-the-literature
#13
Gabriel Luíz Costa, Lara Cotta Amaral, Cor Jesus Fernandes Fontes, Luzia Helena Carvalho, Cristiana Ferreira Alves de Brito, Taís Nóbrega de Sousa
BACKGROUND: Parasite resistance to anti-malarials represents a great obstacle for malaria elimination. The majority of studies have investigated the association between single-nucleotide polymorphisms (SNPs) and drug resistance; however, it is becoming clear that the copy number variation (CNV) is also associated with this parasite phenotype. To provide a baseline for molecular surveillance of anti-malarial drug resistance in the Brazilian Amazon, the present study characterized the genetic profile of both markers in the most common genes associated with drug resistance in Plasmodium falciparum and Plasmodium vivax isolates...
April 19, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28420098/development-and-validation-of-a-questionnaire-to-assess-knowledge-threat-and-coping-appraisal-and-intention-to-practice-healthy-behaviors-related-to-non-communicable-diseases-in-the-thai-population
#14
Kanittha Chamroonsawasdi, Suthat Chottanapund, Pravich Tunyasitthisundhorn, Nawaphan Phokaewsuksa, Tassanee Ruksujarit, Pawarat Phasuksathaporn
Non-communicable diseases (NCDs) are important issues in Thailand and health sectors are now focusing on modifiable risks that include cognitive, affective and behavioral factors. This study aimed to develop and validate a questionnaire to assess knowledge about NCDs, threat appraisal, coping appraisal and intention to practice based on protection motivation theory. Content validity was determined by the mean of the item content validity index (I-CVI) from five experts. The questionnaire was pilot tested for difficulty of knowledge items and reliability test using the Kuder-Richardson (KR)-20 and Cronbach's alpha coefficient among 30 Thai adult subjects in the health office for two sub-districts...
April 14, 2017: Behavioral Sciences
https://www.readbyqxmd.com/read/28419899/the-long-term-tsunami-impact-evolution-of-iron-speciation-and-major-elements-concentration-in-tsunami-deposits-from-thailand
#15
Lidia Kozak, Przemyslaw Niedzielski
The article describes the unique studies of the chemical composition changes of new geological object (tsunami deposits in south Thailand - Andaman Sea Coast) during four years (2005-2008) from the beginning of formation of it (deposition of tsunami transported material, 26 December 2004). The chemical composition of the acid leachable fraction of the tsunami deposits has been studied in the scope of concentration macrocompounds - concentration of calcium, magnesium, iron, manganese and iron speciation - the occurrence of Fe(II), Fe(III) and non-ionic iron species described as complexed iron (Fe complex)...
April 15, 2017: Chemosphere
https://www.readbyqxmd.com/read/28416956/mutations-in-streptomycin-resistance-genes-and-their-relationship-to-streptomycin-resistance-and-lineage-of-mycobacterium-tuberculosis-thai-isolates
#16
Yin Moe Hlaing, Pongsri Tongtawe, Pramuan Tapchaisri, Jeeraphong Thanongsaksrikul, Unchana Thawornwan, Buppa Archanachan, Potjanee Srimanote
BACKGROUND: Streptomycin (SM) is recommended by the World Health Organization (WHO) as a part of standard regimens for retreating multidrug-resistant tuberculosis (MDR-TB) cases. The incidence of MDR-TB in retreatment cases was 19% in Thailand. To date, information on SM resistance (SMR) gene mutations correlated to the SMR of Mycobacterium tuberculosis Thai isolates is limited. In this study, the mutations in rpsL, rrs, gidB, and whiB7 were investigated and their association to SMR and the lineage of M...
April 2017: Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28414028/culex-culiciomyia-sasai-diptera-culicidae-senior-synonym-of-cx-spiculothorax-and-a-new-country-record-for-bhutan
#17
Thanari Phanitchakun, Parinya Wilai, Jassada Saingamsook, Rinzin Namgay, Tobgyel Drukpa, Yoshio Tsuda, Catherine Walton, Ralph E Harbach, Pradya Somboon
Culex (Culiciomyia) spiculothorax was described from Thailand based on the presence of spiculation on the thorax of larvae. Adult females are characterized but are indistinguishable from those of related species, such as Cx. pallidothorax. Phylogenetic analysis of mitochondrial oxidase subunit I (COI) sequences revealed that specimens identified as Cx. spiculothorax from Thailand, Japan and Bhutan form a single clade with Cx. sasai from Japan (Kimura 2-parameter genetic distances 0-0.9%) that is clearly distinct from clades comprised of other species of subgenus Culiciomyia...
April 13, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28414006/unit-cost-of-common-illness-management-a-comparison-between-a-primary-care-unit-and-a-community-pharmacy-in-thailand
#18
Kritsanee Saramunee, Chanuttha Ploylearmsang, Surasak Chaiyasong, Wiraphol Phimarn, Phayom Sookaneknun
Aim This study was to perform unit cost analysis of managing common illnesses comparing between a primary care unit (PCU) and a community pharmacy. BACKGROUND: PCU is a key point of access for primary care in Thailand. Although a community pharmacy is an ideal setting, it has not been successfully incorporated in Thailand's health service. Common illnesses are encountered everyday by community pharmacists, an appropriate compensation for this service has not been established. METHODS: A primary care service of one educational institution was a study site...
April 17, 2017: Primary Health Care Research & Development
https://www.readbyqxmd.com/read/28413166/health-literacy-scale-and-causal-model-of-childhood-overweight
#19
Ungsinun Intarakamhang, Patrawut Intarakamhang
BACKGROUND: WHO focuses on developing health literacy (HL) referring to cognitive and social skills. Our objectives were to develop a scale for evaluating the HL level of Thai childhood overweight, and develop a path model of health behavior (HB) for preventing obesity. STUDY DESIGN: A cross-sectional study. METHODS: This research used a mixed method. Overall, 2,000 school students were aged 9 to 14 yr collected by stratified random sampling from all parts of Thailand in 2014...
January 28, 2017: Journal of Research in Health Sciences
https://www.readbyqxmd.com/read/28412524/antimicrobial-resistance-and-genetic-diversity-of-the-sxt-element-in-vibrio-cholerae-from-clinical-and-environmental-water-samples-in-northeastern-thailand
#20
Wanida Mala, Kiatichai Faksri, Kittipan Samerpitak, Umaporn Yordpratum, Wanlop Kaewkes, Unchalee Tattawasart, Chariya Chomvarin
Multidrug resistance in V. cholerae has been increasing around the world including northeastern Thailand. The aquatic environment is a reservoir of V. cholerae and might be an important source of resistant strains. The aims of this study were to investigate the phylogenetic relationships of intSXT gene sequences from 31 clinical and 14 environmental V. cholerae O1 and non-O1/non-O139 isolates and 11 sequences amplified directly from environmental water samples. We also amplified class 1 integrons, the SXT elements (targeting the intSXT gene) and antimicrobial resistance genes directly from water samples...
April 12, 2017: Infection, Genetics and Evolution
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