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https://www.readbyqxmd.com/read/29223715/development-of-two-fluorine-18-labeled-pet-radioligands-targeting-pde10a-and-in-vivo-pet-evaluation-in-nonhuman-primates
#1
Vladimir Stepanov, Akihiro Takano, Ryuji Nakao, Nahid Amini, Shotaro Miura, Tomoaki Hasui, Haruhide Kimura, Takahiko Taniguchi, Christer Halldin
INTRODUCTION: Phosphodiesterase 10A (PDE10A) is a member of the PDE enzyme family that degrades cyclic adenosine and guanosine monophosphates (cAMP and cGMP). Based on the successful development of [11C]T-773 as PDE10A positron emission tomography (PET) radioligand, in this study our aim was to develop and evaluate fluorine-18 analogs of [11C]T-773. METHODS: [18F]FM-T-773-d2 and [18F]FE-T-773-d4 were synthesized from the same precursor used for 11C-labeling of T-773 in a two-step approach via 18F-fluoromethylation and 18F-fluoroethylation, respectively, using corresponding deuterated synthons...
October 23, 2017: Nuclear Medicine and Biology
https://www.readbyqxmd.com/read/29223408/clinical-outcomes-to-exercise-training-in-type-1-diabetes-a-systematic-review-and-meta-analysis
#2
D Jewiss, C Ostman, N King, N A Smart
AIMS: To establish the relationship between exercise training and clinical outcomes in people with type I diabetes. METHODS: Studies were identified through a MEDLINE search strategy, Cochrane Controlled Trials Registry, CINAHL, SPORTDiscus and Science Citation Index. The search strategy included a mix of key concepts related to exercise training; type 1 diabetes; glycaemic control for trials of exercise training in people with type 1 diabetes. Searches were limited to prospective randomized or controlled trials of exercise training in humans with type 1 diabetes lasting 12 weeks or more...
December 6, 2017: Diabetes Research and Clinical Practice
https://www.readbyqxmd.com/read/29223046/cohort-friendly-protocol-for-a-sensitive-and-fast-method-for-trihalomethanes-in-urine-using-gas-chromatography-triple-quadrupole-mass-spectrometry
#3
Pantelis Charisiadis, Konstantinos C Makris
A cohort-friendly biomonitoring protocol has been developed for measuring biomarkers of exposure to disinfection by-products (trihalomethanes, THM) in urine using small initial volume (3mL) and short analysis time (∼10min) that facilitates the throughput of a large number of samples. The objective of this study was to optimise a cohort-friendly biomonitoring protocol for the determination of four THM analytes in human urine using gas chromatography coupled with triple quadrupole mass spectrometry (GC-QqQ-MS/MS)...
December 6, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29221904/tensile-behaviour-of-individual-fibre-bundles-in-the-human-lumbar-anulus-fibrosus
#4
Diana T Pham, Joe G Shapter, John J Costi
Disc degeneration is a common medical affliction whose origins are not fully understood. An improved understanding of its underlying mechanisms could lead to the development of more effective treatments. The aim of this paper was to investigate the effect of (1) degeneration, (2) circumferential region and (3) strain rate on the microscale mechanical properties (toe region modulus, linear modulus, extensibility, phase angle) of individual fibre bundles in the anulus fibrosus lamellae of the human intervertebral disc...
November 28, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/29221866/a-capillary-zone-electrophoresis-method-for-detection-of-apolipoprotein-c-iii-glycoforms-and-other-related-artifactually-modified-species
#5
Coralie Ruel, Marco Morani, Arnaud Bruneel, Christophe Junot, Myriam Taverna, François Fenaille, Thuy Tran
ApolipoproteinC-III (ApoC-III) is a human plasma glycoprotein whose O-glycosylation can be altered as a result of congenital disorders of glycosylation (CDG). ApoC-III exhibits three major glycoforms whose relative quantification is of utmost importance for the diagnosis of CDG patients. Considering the very close structures of these glycoforms and their tendency to adsorb on the capillary, a thorough optimization of capillary electrophoresis (CE) parameters including preconditioning and in-between rinsing procedures was required to efficiently separate all the ApoC-III glycoforms...
December 5, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29220800/exposure-to-traffic-related-air-pollution-and-the-composition-of-the-gut-microbiota-in-overweight-and-obese-adolescents
#6
Tanya L Alderete, Roshonda B Jones, Zhanghua Chen, Jeniffer S Kim, Rima Habre, Frederick Lurmann, Frank D Gilliland, Michael I Goran
BACKGROUND: Traffic-related air pollution (TRAP) exposure has been linked to type 2 diabetes and metabolic dysfunction in humans. Animal studies suggest that air pollutants may alter the composition of the gut microbiota, which may negatively impact metabolic health through changes in the composition and/or function of the gut microbiome. OBJECTIVES: The primary aim of this study was to determine whether elevated TRAP exposure was correlated with gut bacterial taxa in overweight and obese adolescents from the Meta-AIR (Metabolic and Asthma Incidence Research) study...
December 5, 2017: Environmental Research
https://www.readbyqxmd.com/read/29220786/magnetic-susceptibility-of-spider-webs-as-a-proxy-of-airborne-metal-pollution
#7
Marzena Rachwał, Justyna Rybak, Wioletta Rogula-Kozłowska
The purpose of this pilot study was to test spider webs as a fast tool for magnetic biomonitoring of air pollution. The study involved the investigation of webs made by four types of spiders: Pholcus phalangioides (Pholcidae), Eratigena atrica and Agelena labirynthica (Agelenidae) and Linyphia triangularis (Linyphiidae). These webs were obtained from outdoor and indoor study sites. Compared to the clean reference webs, an increase was observed in the values of magnetic susceptibility in the webs sampled from both indoor and outdoor sites, which indicates contamination by anthropogenically produced pollution particles that contain ferrimagnetic iron minerals...
December 5, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29220505/metabolic-characteristics-of-recently-diagnosed-adult-onset-autoimmune-diabetes-mellitus
#8
Oana P Zaharia, Pavel Bobrov, Klaus Strassburger, Kálmán Bódis, Yanislava Karusheva, Michaela Scholz, Daniel F Markgraf, Volker Burkart, Nanette C Schloot, Karsten Müssig, Julia Szendroedi, Michael Roden
Context and Objective: Among patients diagnosed with type 2 diabetes, autoimmune diabetes often remains undetected. Metabolic features of these patients are insufficiently characterized at present. Design, Setting and Patients: This study compared age- and sex-matched adult (41-62 years) humans with recent-onset diabetes: patients positive for antibodies against glutamic acid decarboxylase (GAD) and/or cytoplasmic islet-cell antigen with an insulin-free period of >6 months (ab+/ins-; previously termed latent autoimmune diabetes of adults, LADA), type 1 (ab+/ins+), type 2 diabetes (ab-/ins-) and glucose-tolerant humans (controls) of the German Diabetes Study (n=41/group)...
December 6, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/29220491/cnvcaller-highly-efficient-and-widely-applicable-software-for-detecting-copy-number-variations-in-large-populations
#9
Xihong Wang, Zhuqing Zheng, Yudong Cai, Ting Chen, Chao Li, Weiwei Fu, Yu Jiang
Background: The increasing amount of sequencing data available for a wide variety of species can be theoretically used for detecting copy number variations (CNVs) at the population level. However, the growing sample sizes and the divergent complexity of non-human genomes challenge the efficiency and robustness of current human-oriented CNV detection methods. Results: Here, we present CNVcaller, a read-depth method for discovering CNVs in population sequencing data...
December 4, 2017: GigaScience
https://www.readbyqxmd.com/read/29218879/an-ultra-fast-and-scalable-quantification-pipeline-for-transposable-elements-from-next-generation-sequencing-data
#10
Hyun-Hwan Jeong, Hari Krishna Yalamanchili, Caiwei Guo, Joshua M Shulman, Zhandong Liu
Transposable elements (TEs) are DNA sequences which are capable of moving from one location to another and represent a large proportion (45%) of the human genome. TEs have functional roles in a variety of biological phenomena such as cancer, neurodegenerative disease, and aging. Rapid development in RNA-sequencing technology has enabled us, for the first time, to study the activity of TE at the systems level.However, efficient TE analysis tools are not yet developed. In this work, we developed SalmonTE, a fast and reliable pipeline for the quantification of TEs from RNA-seq data...
2018: Pacific Symposium on Biocomputing
https://www.readbyqxmd.com/read/29217665/critical-determinants-of-combined-sprint-and-endurance-performance-an-integrative-analysis-from-muscle-fiber-to-the-human-body
#11
Stephan van der Zwaard, Willem J van der Laarse, Guido Weide, Frank W Bloemers, Mathijs J Hofmijster, Koen Levels, Dionne A Noordhof, Jos J de Koning, Cornelis J de Ruiter, Richard T Jaspers
Optimizing physical performance is a major goal in current physiology. However, basic understanding of combining high sprint and endurance performance is currently lacking. This study identifies critical determinants of combined sprint and endurance performance using multiple regression analyses of physiologic determinants at different biologic levels. Cyclists, including 6 international sprint, 8 team pursuit, and 14 road cyclists, completed a Wingate test and 15-km time trial to obtain sprint and endurance performance results, respectively...
December 7, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29217201/expression-and-purification-of-the-human-epidermal-growth-factor-receptor-extracellular-domain
#12
Linpeng Wang, Jing Yan, Jingyun Yan, Huanhuan Xu, Dengyang Zhang, Xuanjun Wang, Jun Sheng
In the fields of drug discovery and protein science, small quantities of proteins are always needed to investigate or validate protein-protein (or protein-small molecule) interactions. Traditional transient or stable expression method to obtain recombinant proteins in eukaryotic systems can be laborious and time-consuming, especially when multiple protein variants are required. Here, we present a fast and convenient method for obtaining small quantities of recombinant human epidermal growth factor receptor (rhEGFR) ectodomain protein, which could be efficiently extended to the expression of other eukaryotic proteins...
December 4, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/29216566/new-platinum-ii-and-palladium-ii-complexes-of-coumarin-thiazole-schiff-base-with-a-fluorescent-chemosensor-properties-synthesis-spectroscopic-characterization-x-ray-structure-determination-in-vitro-anticancer-activity-on-various-human-carcinoma-cell-lines-and
#13
Ömer Şahin, Ümmühan Özmen Özdemir, Nurgül Seferoğlu, Zuhal Karagöz Genc, Kerem Kaya, Burcu Aydıner, Suat Tekin, Zeynel Seferoğlu
A new coumarin-thiazole based Schiff base (Ligand, L) and its Pd(II), Pt(II) complexes; ([Pd(L)2] and [Pt(L)2]), were synthesized and characterized using spectrophotometric techniques (NMR, IR, UV-vis, LC-MS), magnetic moment, and conductivity measurements. A single crystal X-ray analysis for only L was done. The crystals of L have monoclinic crystal system and P21/c space group. To gain insight into the structure of L and its complexes, we used density functional theory (DFT) method to optimize the molecules...
November 22, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29215325/dosikit-a-new-portable-immunoassay-for-fast-external-irradiation-biodosimetry
#14
Julie Bensimon Etzol, Marco Valente, Sandrine Altmeyer, Caroline Bettencourt, Samuel Bouvet, Guillaume Cosler, François Desangles, Michel Drouet, Fabrice Entine, Francis Hérodin, Flora Jourquin, Yannick Lecompte, Patrick Martigne, Xavier Michel, Jérôme Pateux, Nicolas Ugolin, Sylvie Chevillard
DosiKit is a new field-radiation biodosimetry immunoassay for rapid triage of individuals exposed to external total-body radiation. Here, we report on the validation of this immunoassay in human blood cell extracts 0.5 h after in vitro exposure to 137Cs gamma rays, using γ-H2AX analysis. First, calibration curves were established for five donors at doses ranging from 0 to 10 Gy and dose rates ranging from ∼0.8 to ∼3 Gy/min. The calibration curves, together with a γ-H2AX peptide scale, enabled the definition of inter-experimental correction factors...
December 7, 2017: Radiation Research
https://www.readbyqxmd.com/read/29215148/31-p-t2-s-of-phosphomonoesters-phosphodiesters-and-inorganic-phosphate-in-the-human-brain-at-7t
#15
Wybe J M van der Kemp, Dennis W J Klomp, Jannie P Wijnen
PURPOSE: To determine the phosphorus-31 T2 s of phosphomonoesters, phosphodiesters, and inorganic phosphate in the healthy human brain at 7T. METHODS: A 3D chemical shift imaging multi-echo sequence with composite block pulses for refocusing was used to measure one free induction decay (FID) and seven full echoes with an echo spacing of 45 ms on the brain of nine healthy volunteers (age range 22-45 years; average age 27 ± 8 years). Spectral fitting was used to determine the change in metabolic signal amplitude with echo time...
December 7, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/29215041/crispr-cas9-delivery-with-one-single-adenoviral-vector-devoid-of-all-viral-genes
#16
Eric Ehrke-Schulz, Maren Schiwon, Theo Leitner, Stephan Dávid, Thorsten Bergmann, Jing Liu, Anja Ehrhardt
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system revolutionized the field of gene editing but viral delivery of the CRISPR/Cas9 system has not been fully explored. Here we adapted clinically relevant high-capacity adenoviral vectors (HCAdV) devoid of all viral genes for the delivery of the CRISPR/Cas9 machinery using a single viral vector. We present a platform enabling fast transfer of the Cas9 gene and gRNA expression units into the HCAdV genome including the option to choose between constitutive or inducible Cas9 expression and gRNA multiplexing...
December 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29214631/system-matrix-computation-vs-storage-on-gpu-a-comparative-study-in-cone-beam-ct
#17
Dmitri Matenine, Geoffroi Côté, Julia Mascolo-Fortin, Yves Goussard, Philippe Després
PURPOSE: Iterative reconstruction algorithms in computed tomography (CT) require a fast method for computing the intersection distances between the trajectories of photons and the object, also called ray-tracing or system matrix computation. This work focused on the thin-ray model and is aimed at comparing different system matrix handling strategies using graphical processing units (GPUs). METHODS: In this work, the system matrix is modeled by thin rays intersecting a regular grid of box-shaped voxels, known to be an accurate representation of the forward projection operator in CT...
December 6, 2017: Medical Physics
https://www.readbyqxmd.com/read/29214390/changes-in-motor-performance-and-mental-workload-during%C3%A2-practice-of-reaching-movements-a-team-dynamics-perspective
#18
Isabelle M Shuggi, Patricia A Shewokis, Jeffrey W Herrmann, Rodolphe J Gentili
Few investigations have examined mental workload during motor practice or learning in a context of team dynamics. This study examines the underlying cognitive-motor processes of motor practice by assessing the changes in motor performance and mental workload during practice of reaching movements. Individuals moved a robotic arm to reach targets as fast and as straight as possible while satisfying the task requirement of avoiding a collision between the end-effector and the workspace limits. Individuals practiced the task either alone (HA group) or with a synthetic teammate (HRT group), which regulated the effector velocity to help satisfy the task requirements...
December 6, 2017: Experimental Brain Research. Experimentelle Hirnforschung. Expérimentation Cérébrale
https://www.readbyqxmd.com/read/29213292/ifn-%C3%AE-1-in-cho-cells-its-expression-and-biological-activity
#19
Wu-Mei Yuan, Wan-Ju Zhang, Fen-Lian Ma, Jin-Song Li, Qian Zhang, Li-Shu Zheng
Background: Many studies have investigated the characteristics and biological activities of type III interferon (IFN), finding that it has similar features to type I IFN but also unique actions because it is recognized by a different receptor. Results: A full-length recombinant human IFN-λ1 (rhIFN-λ1) cDNA was cloned into the pDF expression vector and stably expressed in Flp-In-CHO cells. After four purification steps (ammonium sulfate precipitation, SP Sepharose chromatography, Blue Sepharose 6 fast flow affinity chromatography and molecular sieve chromatography), the rhIFN-λ1 had a purity of about 90% and was found to have the predicted biological activities...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/29213238/electrophysiological-and-pharmacological-analyses-of-nav1-9-voltage-gated-sodium-channel-by-establishing-a-heterologous-expression-system
#20
Xi Zhou, Zhen Xiao, Yan Xu, Yunxiao Zhang, Dongfang Tang, Xinzhou Wu, Cheng Tang, Minzhi Chen, Xiaoliu Shi, Ping Chen, Songping Liang, Zhonghua Liu
Nav1. 9 voltage-gated sodium channel is preferentially expressed in peripheral nociceptive neurons. Recent progresses have proved its role in pain sensation, but our understanding of Nav1.9, in general, has lagged behind because of limitations in heterologous expression in mammal cells. In this work, functional expression of human Nav1.9 (hNav1.9) was achieved by fusing GFP to the C-terminal of hNav1.9 in ND7/23 cells, which has been proved to be a reliable method to the electrophysiological and pharmacological studies of hNav1...
2017: Frontiers in Pharmacology
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